Questions related to Infrared Microspectroscopy
I know of several for the gas phase (e.g. HITRAN, GEISA, PNNL) but not the condensed phase. Most seem to be proprietary databases for matching spectra, but don't allow determining absorption as a function of density or path length.
Due to the wavelength-dependent penetration depth of IR light in a sample, ATR spectra exhibit differential absorption band intensities, but what might be causing (constant and sloping: linear/exponential) baselines in spectra recorded via ATR? As far as I understand, it cannot be due to scattering/interference effects, as is the case in transmission/transflection setups.
I have not been able to find a clear answer in literature, so any references are always welcome.
Thank you in advance.
I am currently applying micro-ATR (Bruker Hyperion 2000 microscope) on microscopic, single-cell biological and palaeontological dinoflagellate cysts.
Apart from atmospheric and baseline corrections, Savitzky-Golay smoothing and extended ATR correction (OPUS software, for wavelength-dependent penetration depth), are there any other preprocessing steps you can undertake (and in what order) to improve further qualitative analysis of the data? Is there an 'optimal' protocol in existence?
Are there any differences with macro-ATR data (preprocessing)?
Thanks in advance.
I have 5 samples cut from the same epoxy film. I perform FTIR-ATR on them 4 times per film before and after exposure to water. So in all, I have 20 FTIR spectra of one film in one condition. If I want to compare the water (OH) peak, should I average all the spectra first and then normalize the resultant spectrum, or should I normalize the OH peak in each spectra and then average it? If I do the latter, the standard deviation comes out to less than 0.1.
I wanted to get a feedback on the use of infrared or Raman spectroscopy for detection of breast, cervical lung cancers or in detecting blood biochemistry.
Has anybody used the method for detection of markers such as EGF, p16, CEA, CA125 for early cancer detection?
I'm interested in performing mid-IR (4-12 um) spectroscopy in microfluidic devices. Are there any spectrometers that can couple into the small volumes and deal with the short time scales present in such devices?
I am looking for a fast frame rate, high quality mid-infrared camera (5-12um ideally). Does anyone have experience that could provide a recommendation? I would also be looking for somewhat tunable QCLs in this range if you have suggestions there even better.