Science topic

Infertility - Science topic

Infertility primarily refers to the biological inability of a person to contribute to conception.
Questions related to Infertility
  • asked a question related to Infertility
Question
2 answers
Recently, I have researched the health-related quality of life and psychological health of infertility patients. Here I am Looking for your kind opinion on which journals would be best to submit a research paper on such topics.
Relevant answer
Answer
You may submit to journals like BMC Medical Educatio, Plos One
  • asked a question related to Infertility
Question
2 answers
It is no secret to everyone that most of the causes of infertility are genetic, but there is little research in this field that needs answers supported by research and studies concerning the genes that cause infertility, especially in women.
Relevant answer
Answer
Dear Dr :Asawer Kareem
looking forward to read good answer and participation . thank you for this question .
  • asked a question related to Infertility
Question
6 answers
Most regular male condoms contains anti-spermicidal agent. The woman is looking for a child. It is been used as a probe sheath for TVS.
Relevant answer
Answer
Apologies, I meant condoms with spermicidal agents. Probe sheaths ideally should be specially made for TVS. However, NONn_spermicidal condoms can be improvised and be used as probe sheaths. Depending on the timing of the ultrasound scan and uncertainty of the time at ovulation. If the individual have coitus at the time of TVS, use of a condom containing spermicides can mitigate the whole essence of fertility treatment. However , because of the unavailability of spermicidal free condoms, most sonologist/Gynecologyist tend to use what is available to them which is spermicidal condoms as probe sheaths. Does this matter?
  • asked a question related to Infertility
Question
3 answers
Infertility cases such as PCOS or unexplained infertility in step before IVF ,we try monofollicular stimulation with ovulation induction medication.
What is the guidelines?
Steps in management?
Relevant answer
Answer
letrozole 3/DAY for 4 days and USscan after 7days from start. works well;
cheers
  • asked a question related to Infertility
Question
2 answers
Azoospermia, severe oligozoospermia, moderate oligozoospermia, or mild oligozoospermia are all symptoms of Y chromosome infertility. Males with Y chromosome infertility usually do not exhibit any obvious symptoms, though physical examination may reveal small testes. For genetic testing, it is essential to isolate the Y-chromosomes completely from the semen to determine the chromosomal abnormality. My question is, what is the existing methods and what would be the novel strategies owing to the progression of molecular biology and nanotechnology?
The question is open to all interested researchers.
Thank you in advance.
Relevant answer
Answer
Thank you Prof. Haitam for suggesting the article. It is quite informative.
  • asked a question related to Infertility
Question
3 answers
How to do CRISPR CAS 9 Sampling from Infertile individuals in order to check genetic mutations(genetic reason for infertility both male and female)?
Relevant answer
Answer
HEREDITICS: HEREDITARY DISEASES ARE BOTH GENETIC AND CYTOHETIC DEFECT
Dear Yasir Riaz,
Genetic mutation will cause disease. It is according to the theory of Genetics. However, as a general people, there are a lot of genetic mutaion happened in our cells during DNA replication. Why do we not get hereditary diseases? Because there is heredity control system, i.e. cell cycle checkpoint and licensing system in every cell of our body. The system is mainly located in cytoplasm. The theory that cytoplasm containing heredity control function is called Cytohetics.
CRISPR techniques depend on the precise function of the specific enzyme. However, none of those enzymes in our bodies and in the lab are perfect enough not produce any mistake at the rate of 99.999%. That is why our cells contian heredity control system to maintain the correctness of our heredity not to be changed since there is human beings for about 5 million years. As we know, ICSI may produce about 1% chromosomal abnormality due to disturb microtubules. Embryo biopsy and laser biopsy has produced Embryo Biopsy Syndrome with neuropathy problems. Ooplasm transfer has produced MRT Syndrome. It is foreseeable that CRISPR will also produce CRISPR Syndrome. To treat infertility problem and produce new cytohetic and new genetic diseases at the same time is absolutely not a scientific practice. Cytohetic diseases are also hereditary diseases.
Please read https://www.hereditics.net to understand a complete study of heredity - Hereditics. If we wish to get a healthy and clever child, don't disturb the cytoplasm and genome at the level of germline.
Sincerely yours,
Ke-Hui Cui, M.D., Ph.D.
  • asked a question related to Infertility
Question
5 answers
Regarding "NANOTECHNOLOGY in Animal Reproduction", I have just started reading the critical literature along with compiling the relevant papers available on the net. For writing up a BOOK Chapter, I basically need some helpful advice and references that could be kindly provided hereby, as appropriate. Thank you all.
Relevant answer
Answer
As a principle artificially made medias for preservation and cryoprotection is to imitate the natural environment. The Nanotechnology would enable us to understand the detail constituents of the natural environment to further tuning the artificially made medias for preservation and cryoprotection.
  • asked a question related to Infertility
Question
9 answers
It has been proven that those vaccinated in the new vaccines excrete spike proteins through breath, saliva and urine. According to my inquiry to the Federal Environment Agency, there is no research on what these excretions could cause in the long term, e.g. via the water cycle, for damage to nature and the environment - as it is well known from other drug residues, in particular from the birth control pill, the infertility of fish and Amphibians in part. If so much is being vaccinated globally with the new vaccine - can one estimate the risk of long-term serious damage to nature and the environment without prior research for whatever reason? How long do z. B. These spike proteins outside the body and what do they break down into?
Relevant answer
Answer
The spike protein degraded in body within72 hrs and act as immunogen only for promoting immunolgical system to form antibodies againest spike protein(virulent part of virus or part of virus responsible for invasion and infection)
  • asked a question related to Infertility
Question
3 answers
Discriminant function for the prediction of spontaneous pregnancy and table (table 4) for the finding of appropriate % are given in the preprint entitled "Prediction of reproductive function recovery after microsurgical varicocelectomy in men from infertile couples: Clinical and laboratory predictors"
Relevant answer
Answer
Dear doctor Azizbek,
Sure sir, if the varicocele repair is done by accurate and clean surgery or with out complications. Hence we expected for a high percent of pregnancy occurring.
  • asked a question related to Infertility
Question
4 answers
A 28 years old female presented with 7 years infertility with no obvious reason for her infertility.
her hormonal analysis with husband seminal analysis is quiet normal. Apart from long fallopian tube, there where no any obvious cause for her infertility's state. The hysterosalpingography result shows long fallopian tubes with its direction down word in the pelvis and the uterus located just above it, although they were patent.
Relevant answer
Answer
Tumur Baldan , thank you for your reply, yes of course it could be. However, she has good developing ova, and it has been 7 years at least some cycles the ovulation is good..
  • asked a question related to Infertility
Question
5 answers
Varicocelectomy leads to an improvement in semen parameters only in 60-70% of cases, and real fertility in 30-60% of cases (Abdel-Meguid et al., 2011, Samplaski et al., 2017, Almekaty et al., 2019).
Relevant answer
Answer
As for male reason, as for some female reasons or both, subfertile couples need to recognise the natural fertile window in every menstrual cycle. The best and cheapest method to reconnoitre it is the symptothermal method.
  • asked a question related to Infertility
Question
4 answers
we know that FSH is vital for spermatogenesis and studies have shown that FSHB promoter variant 211G/T is associated with low levels of FSH.
2) 10.1371/journal.pone.0094244
Relevant answer
Answer
Kindly find the attached research paper.
  • asked a question related to Infertility
Question
1 answer
I am working on a project where I am trying to knockout a gene of interest then knockin the same gene but from another species to determine if it can still produce viable proteins using microinjection. I am unable to do a knockout in the first generation then use a knockin for the next generation as my knockout will cause infertility. Is there a way to do this simultaneously?
Relevant answer
Answer
These guys knocked in a ~200kb human locus in place of the orthologous rat locus (see Fig 4), which sounds analogous to what you're proposing to do:
So, yes.
Depending on the size of the original target, you could alternatively consider knocking in the replacement ortholog as a one-way flippable lox66-cDNA-lox71 cassette into an intron the opposite orientation to your target gene. That way the potential for sterility wouldn't be a concern until the switch was flipped by Cre expression.
  • asked a question related to Infertility
Question
27 answers
Climate change and agriculture are interrelated processes, both of which take place on a global scale. Climate change affects agriculture in a number of ways, including through changes in average temperatures, rainfall, and climate extremes (e.g., heat waves); changes in pests and diseases; changes in atmospheric carbon dioxide and ground-level ozone concentrations; changes in the nutritional quality of some foods;[1] and changes in sea level.[2]
Climate change is already affecting agriculture, with effects unevenly distributed across the world.[3]Future climate change will likely negatively affect crop production in low latitude countries, while effects in northern latitudes may be positive or negative.[3] Climate change will probably increase the risk of food insecurity for some vulnerable groups, such as the poor.[4] Animal agriculture is also responsible for CO2 greenhouse gas production and a percentage of the world's methane, and future land infertility, and the displacement of local species.
Relevant answer
Answer
The rise in temperature leads to an increase in the amount of water loss through Evaporation and transpiration. Therefore, greater quantities of water are needed for agricultural production, and higher temperatures lead to an increase in fungal and viral diseases and insect infestation.
I think that high temperatures are more dangerous than a reduced rainfall
  • asked a question related to Infertility
Question
4 answers
Has anyone been able to successfully cryopreserve coral oocytes? I've only found one case for a Gorgonian (Junceella juncea) through vitrification (Tsai et al. 2015, attached below). I know that in some cases some researchers (personal communications) have been able to thaw and retrieve from liquid nitrogen, but though they are still 'alive', they loose their ability to be fertilized (infertile).
I appreciate your help providing me with your answers and/or experience in this matter.
Relevant answer
Everything is very simple. They are prepared in the same way as mammalian eggs. Deep freezing takes place in liquid nitrogen. There is also an article, unfortunately in Russian.
  • asked a question related to Infertility
Question
1 answer
I have already mentioned that i am one of the author of Mymensing med J 2014 Oct;23(4):770-773. Article titled "Association of infertile patients having polycystic ovarian syndrome with recurrent miscarriage." Research gate showing different name. that is Sanam Begum. How i will overcome this problem?
Relevant answer
Answer
Mam, is this article included in your profile? This kind of incidence could happen, if your co-authors added that name. You can again add this article.
  • asked a question related to Infertility
Question
5 answers
I noticed this finding, where after laparoscopy or hysteroscopy, a hemorrhagic ovarian cyst occurred during the next cycle that was about 4 cm. Is there a relation? Any explanation is appreciated!
Relevant answer
Answer
Yes, I also came across a case of a hemorrhagic cyst in the ovary after laparoscopic cholecystectomy. The reason may be that pneumoperitoneum created during the surgery may cause rupture of fine vessels in the ovary But this may be self-limiting.
  • asked a question related to Infertility
Question
13 answers
Male Infertility is a major public health concern worldwide and there had been heated debates to date on the above question; in other words is Boxers vs. Briefs related to Increasing Sperm Count ?
Please what is your take on this crucial public health issue?
Relevant answer
Answer
“In the largest study of its kind to date, the researchers found men attending a fertility clinic with looser-fitting boxer shorts had higher sperm concentration, a 17% higher total sperm count and 33% more swimming sperm than men with tighter-fitting underwear.”
Please what is your take on the crucial health issue?
  • asked a question related to Infertility
Question
2 answers
hormone of prolactin was secreted by pituitary gland located in neck during lactation. Which encourage to produce milk. but infertile women become high. Please tell us the relation?
Relevant answer
Answer
The most common hypothesis to explain the link between HPRL and PCOS is a possibly common hypothalamic-pituitary abnormality that can explain both PCOS and hyperprolactinemia.
  • asked a question related to Infertility
Question
11 answers
Does medically treated hydrosalpinx could improve pregnancy rate by natural way.
Hydrosalpinx for a patient complaining from secondary infertility have been treated over a period of 3 months with different courses of antibiotic according to culture until no complain or discharge remain and 2nd hystrosalpingeography done, one tube patent and other have feature of hydrosalpinx, does this necessary still have infection? Does this course of treatment overcome failure rate of pregnancy with hydrosalpinx?.
Relevant answer
Answer
"A woman who is not able to give her husband
a child faces the danger of being divorced.
This fear is real while she is subjected to
psychological abuse; her husband threatens and
blames her." Nice such a loving till-death-us-parts-partner. What about:
"A man who is not able to give his wife
a child faces the danger of being divorced.
This fear is real while he is subjected to
psychological abuse; his wife threatens to poison him and
blames him." Could you write this in Iran? (with 1/3 of the subfertile couples there is something wrong with the men, moreover infertility of women is in mijn experience not seldom related to blocked tubes, related to an infection she got via her partner)
Is it possible to liberate yourself somewhat? It seems that the problem is not so much infertility, there are in many cultures women who elect not to have children and get satisfaction from a career for example, but the attitude from the husband, no loving support and understanding.
Be on more equal terms with men.
And if infertility is such a serious problem "major health impact" nationally that perhaps all the effort and money invested in nuclear weapons could be better employed in the apparently very serious problem of infertility. All that money for development of arms, security, boycotts would be enough to have 5 IVF teams in every hospital in Iran.
  • asked a question related to Infertility
Question
4 answers
Dear everyone,
I'm doing research on infertility and need to collect sperm from male mice's caudal epididymis. Sometimes the results are good but sometimes we can not get any sperms. I have been looking for the guideline or training documents on mice's reproductive engineering techniques but I really can not find any well-prepared ones. Can any specialist in this field help to give me a hint or a source of information about this technical issue?
Thank you very much for reading. Looking forward to hearing from you.
Relevant answer
Answer
Your answers really made the problem clear, Vasanth Thamodaran and Nguyen Xuan Phuoc . Besides, is there any source of reference images for the mice sperms' morphology and abnormality (and human also, if available) in the caput, cauda and vas Deferens, respectively?
Thank you very much for your replies.
  • asked a question related to Infertility
Question
7 answers
The period of fertility in birds is correlated with the population of sperm-filled SSTs in the UVJ.
To achieve fertilization, the resident sperm must be released from the SSTs in order to migrate to the site of fertilization, which is infundibulum part of the oviduct.
Relevant answer
Answer
Thanks for your reply. But each of these sources points to several mechanisms. But none of them have identified the most important mechanism. In other words, it is still unclear what is the main mechanism of sperm retention for 14-7 days in the bird's oviduct.
  • asked a question related to Infertility
Question
7 answers
How endometriosis cause sub-fertility?
Relevant answer
Answer
Endometriosis can cause infertility through different mechanisms : 1. Pelvic, tubal and uterine adhesions. 2. endometrial tissues secreted toxic substances which negatively affect spermatoza and decrease their fertilizing ability. 3. Vaginisimus, vaginal nodules or mass. 4. Ovulatory dysfunction in 10% of cases.
  • asked a question related to Infertility
Question
5 answers
IUI is one of the assisted reproduction techniques which are used in certain sub-fertility cases to increase the chance of pregnancy.
Relevant answer
Answer
1. Tubal obstruction or tubal pathology. 2.sever genital tract infection of both partners. 3.sever impairment of semen parameters. 4.pelvic surgery, pathology or adhesions. 5 undetermined vaginal bleeding. 6.cervucal stenosis, pathology or cervicitis. 7.coexcisting infertility problems. 8 advanced female age.
  • asked a question related to Infertility
Question
1 answer
I am grateful to friends across the globe for patronage to Semen Fructose Estimation Kit developed by me and manufactured by my family enterprise Maa Vaishno diagnostics. Perhaps marketing and procurement problems have been a bottleneck in use of this method of immense importance in cattle and human infertility.
On National Republic Day of year 2020, I am pleased to declare my Reagent Formulation open to public use.
Diagnostic laboratories may prepare and use my Reagent.
Diagnostic companies may manufacture and distribute; no royalty expected.
Method described below and also viewable at http://indianmedical.net/fructose
Reagents 1. DoctorKC' Fructose Reagent Indole 3-acetic acid LR  0.5 g Thiourea LR 0.1 g Isopropyl alcohol 250 ml Shake frequently and keep overnight. Filter The reagent is infinitely stable at room temperature.
2. Hydrochloric acid LR 3. Standard : 200 mg/dl 5 ml
Procedure
For Semen Fructose Drop Test in evaluation of Sperm Activity, Standard reading is not requird.
1. Pour over 0.005 ml sample, 5 ml Hydrochloric acid. 2. Add 0.1 ml Fructose Reagent. Mix by inversion. 3. Incubate at 50ºC for exact 50 minutes. 4. Cool and read absorbance of Sample and Standard against Blank adjusted to zero with bluish green filter (500-530 nm).
For standard, take 0.005 ml standard in place of sample in step 1. For blank, omit sample/standard addition. Proceed further with both tubes as with the test.
For Semen Fructose Drop Test, use Sample reading of 30 minutes old sample (Abs30) and 240 minutes old sample (Abs240). 
Calculation                       Abs Sample
Fructose = ------------------------------- x 200 mg/dl                       Abs Standard
Fructose Drop using Standard
Fructose at 30 minutes - Fructose at 240 minutes
Fr Drop  = ------------------------------------------------------------------------------------ x 100 percent         Fructose at 30 minutes
Fructose Drop without Standard
                             Abs30 - Abs240 Fructose drop  = --------------------------------------- x 100 percent                                      Abs30
Relevant answer
Answer
Thank you very for your letter
  • asked a question related to Infertility
Question
5 answers
Infertility is becoming a Social and public problem rather than a medical issue. determining the level of social stigma would benefit health care workers to develop interventions accordingly
Relevant answer
Our new paper may interest you:
  • asked a question related to Infertility
Question
10 answers
Climate change
Infertility
Relevant answer
Answer
The answer to your question is "No". In fact, polycystic ovary syndrome (PCOS) and obesity are the main reasons behind global rise of infertility in women.
  • asked a question related to Infertility
Question
5 answers
What are the genes involved in Polycystic ovary syndrome (pco), what is the role of oxidative stress in progression of PCO?
Relevant answer
Answer
Please take a look at the following RG link.
  • asked a question related to Infertility
  • asked a question related to Infertility
Question
2 answers
what are the reasons for Infertile and empty' seeds in the Cuminum cyminum L?
Relevant answer
Answer
@ Toluwase Righteous Odefemi , thank u for your answer, according to my studies, acclimate factors and Irrigation is most effective than soil properties for Infertile and empty' seeds
  • asked a question related to Infertility
Question
10 answers
I am working on a sperm specific protein, I want to determine its concentration in control and infertile. Can it be done using real-time PCR and how?
Relevant answer
Answer
Western blot can give you information on relative protein amount but is not traditionally considered a 'quantitative' technique. If you want data that's a bit more quantitative, you can use qRT-PCR. Remember though, qRT-PCR is quantifying the amount of specific mRNA species in your sample. So, you can infer protein amount by using this method, but remember.. just because the mRNA is present at a certain level does not guarantee an equally proportionate amount of the corresponding protein is also present.
For qRT-PCR you will need some materials that are unique to this method. The thermal-cycler that is used is a bit different from the traditional machines used in most PCR applications. Check if your department has this equipment. Then you will need to find the appropriate reagents to prepare your reactions. I strongly advise you run each reaction in triplicate (duplicate, at a minimum). I have used the BioRad reagents in the past and it worked well. Thermo also has many products that will work.
Finally, you need to design primers that are complimentary to the mRNA transcript you are probing for. There are online guides available to help with this (e.g. https://bitesizebio.com/10041/designing-qpcr-primers/ )
In case you're not clear about how this process works - qRT-PCR is a method that uses PCR amplification to check for the amount of specific mRNA transcripts in your sample. To do this, you first need to isolate the RNA in your sample. Then the mRNA in your sample must be converted to cDNA (complimentary DNA). Then you can probe the cDNA using primers you have designed. In simple terms, this method will count the number of amplification cycles for each probe. By comparing the number of cycles it takes to amplify different targets (and comparing to a "housekeeping"/reference gene, e.g. GAPDH, actin, 18S, etc), you can quantify the expression level of your target genes.
Workflow summary:
Sample > isolate RNA > cDNA > qRT-PCR
  • asked a question related to Infertility
Question
1 answer
Hi all,
We are going to house mice for a double-ko investigation. Since this is our first time housing mice, we have no knowledge on how we should optimize breeding.
So here's the info:
One gene is on Chr-X, the other on Chr-11. Both males and females homozygous double-ko should be infertile. The simple ko are supposed to be ok. We are mostly interested in ovaries of the double-ko females, but we'll also check on both simple-ko and wt as controls.
So what strains should we ask to start the colony ? We also want to cryopreserve embryo in the first few weeks.
The collaborator who is going to send the mice proposed this:
Males: Gene1(-/0), Gene2(-/+)
Females: Gene1(-/+), Gene2(-/+)
Do you think that is an appropriate start ?
Relevant answer
Answer
In my opinion, If you cross male and female of given pair:
Theoreticallly, Your chances on getting one male double KO and one female double KO in 16 pups if all are alive (you will keep on breeding this pair till you get enough numbers), The details lies here:
(1) 1-/- 2-/- Female Double KO
(2) 1-/0 2-/+ Male
(3) 1-/0 2-/- Male Double KO
(4) 1-/- 2-/+ Female
(5) 1-/- 2-/+ Female
(6) 1-/0 2+/+ Male
(7) 1-/0 2-/+ Male
(8) 1-/- 2+/+ Female
(9) 1-/+ 2-/- Female
(10) 1+/0 2-/+ Male
(11) 1+/0 2-/- Male
(12) 1+/- 2+/- Female
(13) 1-/+ 2-/+ Female
(14) 1+/0 2+/+ Male
(15) 1+/0 2-/+ Male
(16) 1-/+ 2+/+ Female
Once you get a pair of double KOs, and if they are fertile (depending on your genes not affecting male and female fertility), You will be lucky that you can breed among them and get all pups should be double KOs.
Alternatively,
for next breedings, you can may include female mouse number 9 and male number 11 for better results if they are fertile
Hope that helps, let us see if someone comes up with better strategy,
Subhash
  • asked a question related to Infertility
Question
12 answers
hello,
Does anyone knows any molecules that causes infertility in a short time quickly , i want to study the infertility in male and i want to induce infertility in the lab animale so i can study it.
thank you
Relevant answer
Answer
Dragan Uros Crnomarkovic thank you Sir for your answer, may i ask you the book that you are talking about , i think it is very interesting.
  • asked a question related to Infertility
Question
10 answers
Why is polycystic ovary syndrome (PCOS) rising all over the world?
Relevant answer
Answer
PCOS is heavily linked to insulin resistance. With the rise of non-communicable diseases globally including type 2 diabetes and metabolic syndrome, the incidence of PCOS is expected to also increase considerably.
  • asked a question related to Infertility
Question
7 answers
Hello, I want to induce infertility in rats, I want to know the dosage of gossypol to use and how long I will administer the gossypol to achieve infertility. Also, does gossypol induce infertility in female rats?
Relevant answer
Answer
This confirmed anti-fertility parameters
  • asked a question related to Infertility
Question
9 answers
I'm doing a research related with an effectivenss of herbal medicine for unexplained infertility. But, only a prospective observational study design is possible (without control group). In this case, how can I evaluate an effectivenss of herbal medicine for unexplained infertility?( vs expectation managment or IUI)
Now, I'm just plan to compare the pregnancy rate with published prediction models. Is this the best plan?
Relevant answer
what is the type of heterogencity?
is it in genus, age or sex?
  • asked a question related to Infertility
Question
14 answers
I'm trying to get better contrast between sperm cells and the ambient fluid under a light microscope, but I need the sperm to remain alive after staining to evaluate their motility. Is there a good dye for this (does not have to be specifically for spermatozoa)?
Relevant answer
Answer
i went to know the type of the this stains also please
  • asked a question related to Infertility
Question
3 answers
Hi everybody,
I am looking for dubious gene(s) that may causing ovary polycystics?? Some genes are highly expressed in people that are tolerating polycystic ovary or infertility.
Sincerely,
Relevant answer
Answer
CYP11a, CYP21, CYP17, and CYP19,
  • asked a question related to Infertility
Question
5 answers
Is it possible that the issue of infertility can be solved by laser?
If yes then how?
if no what is best way for this kind of issue?
Thanks
Relevant answer
Answer
Yes. I know papers showing the increase of speed of sperm cells and may be it can improve the fertilization process. One of these papers was published in PLoS One. 2015; 10(3): e0121487.
  • asked a question related to Infertility
Question
4 answers
Soil infertility, soil chemistry, physical chemistry, Soil physics
Relevant answer
Answer
Mr. Phogat,
Via MS.
  • asked a question related to Infertility
Question
10 answers
Could the transfer of embryos (or oocytes and zygotes) between different culture media induce alterations in their cytoskeletal structure leading to cell division failure?
Thanks in advance.
Relevant answer
Answer
Dear Gabor, the following paper may help you:
Mechanical Stresses in Embryonic Tissues: Patterns, Morphogenetic Role, and Involvement in Regulatory Feedback
L.V.Beloussov, S.V.SavelievI, .NaumidiV. V.Novoselov
  • asked a question related to Infertility
Question
5 answers
I am trying to determine the extent of transmission of Lassa fever through sexual contact. Any ideas how I can do this? I can analyze semen samples from areas that just witnessed Lassa fever outbreak in Nigeria for virus presence but obtaining these samples is near impossible. Analyzing semen samples submittted to tertiary health facilities for infertility investigation is a possibility, but wont be quite representative as Lassa is commoner in rural areas.
Also, I am at not sure how to measure transmission following sex and/or pregnancy, or adverse birth outcomes follow 'congenital Lassa' if it exists.
Relevant answer
Answer
I think obtaining seminal fluid from Lassa fever patients will be quite difficult. However you can group patients into those in the incubation period (pre-pathogenic phase) Acutely ill persons (pathogenic Phase) and those convalescing (early post pathogenic phase) and those who have survived the illness (Post-pathogenic phase) This will enable us to know the stage at which Lassa fever is transmitted if it is actually sexually transmitted disease. That Lassa fever is transmitted through body fluids implies that it is very likely to be found in the semen except the blood testis barrier is capable of preventing Lassa fever virus from escaping into the seminiferous tubules. Even at that most of the seminal fluid is derived from seminal vesicles (60%), prostate gland (30%) and bulbo-urethral glands(5%) leaving 5% for the sperm cells..
This study will be easier to carry out in a center where there are several Lassa fever patients. Semen can easily be obtained when patients are seen in the clinic (because it becomes a requirement for evaluation), when they are being admitted ( if they are not acutely ill) when they are convalescing, and when they are being discharged. The patients must sign informed consent form. It will be almost impossible to obtain semen when they acutely ill.
However if the virus is found in the semen from the groups mentioned above it can safely be deduced that the the semen is present in the acute phase of the disease.
  • asked a question related to Infertility
Question
4 answers
Is there any specific diagnostic test for the oxidative stress we can recommend in every male infertility case? I need the details and any additional newly developed diagnostic tests for seminal calcium assessment?
Relevant answer
Dear Huda, you can find some information , that can help you in the links below :
  • asked a question related to Infertility
Question
4 answers
we are planning to do sperm DNA fragmentation tests for infertile patients and we can do by SCD and SCSA. interestingly the results varies. if someone has any experience or idea about it please share.
Relevant answer
Answer
Dear Syedwaseem Andrabi
The COMET and Sperm Chromatin Dispersion (SCD; HALO) tests were introduced as light microscope tests that don’t require a flow cytometer. Since these tests measure only 50–200 sperm per sample, they suffer from the lack of the statistical robustness of flow cytometric(5000 sperm) measurements. Only the SCSA test has an exact standardization of a fixed protocol. The many variations of the other tests make it very difficult to compare data and thresholds for risk of male factor infertility.
Regards
Sanketh D.S
  • asked a question related to Infertility
Question
3 answers
We are investigating hormonal and essential trace metal imbalance as aetiological basis of secondary infertility. We need to develop an animal model of secondary infertility. We are searching for drug /chemical that can achieve this by disrupting the GnRH activity
Relevant answer
I am not very clear about the recommended procedure, I wish you could explain more on the procedure, thanks
  • asked a question related to Infertility
Question
6 answers
Any One working on Infertility Genetics?
Regards,
Dr. Syed Irfan Raza
Relevant answer
Answer
Such researches are not at all required on human.
You can perform many genetic researches on birds and animals (as their life span is short) to get the trend which may be found in human. Many studies were performed in the following manner.
1. Half sib and full sib mating ( cross between brother x sister).
2. Test cross.
3. Back cross ( father x daughter, mother x son cross) etc.
By many such studies, it was clear that offspring of close relatives have very high chance of gathering of bad genetic characters ( as homozygous recessive Gene gatheration ) and so chance of delivering abnormal offspring is more.
IT IS SCIENTIFICALLY WRONG TO MARRY IN CLOSE BLOODS.
  • asked a question related to Infertility
Question
6 answers
As you know poly cystic ovaries and infertility is difficult to control in many cases. Sometimes prescribing traditional hormonal drugs and metformin in over weight female with NIDDM is not effective. What is the best therapeutic agents to control these patients?
Relevant answer
Answer
PCOS therapy must be phenotype driven. I consider pregnancy wish as a particular case and I prefer letrozole combined or not with low dosis of FSH.
In the presence of biochemical hyperandrogenism the aim is to increase SHBG, inhibit LH pulsatility and diminish free testosterone. I do prefer EE 35ug combined with 2 mg cyproterone acetate. If necessary, I increase CPA
in 25 mg for 10 days each month. In the presence of glucose intolerance or insulin resistance I have experience only with metformin. In the presence of obesity I use a COC containg drospirenone associated with diet, exercise, and orlistat. If possible 1.8 mg day of liraglutide. In the presence of various phenotypes a combined therapy is indicated.For dislipidemia statins should be used.
  • asked a question related to Infertility
Question
3 answers
I want to examine effect of calcium deficiency in fertility of female mouse. i need a protocol for creating calcium deficiency. 
Relevant answer
Answer
You have to treat animals with calcium deficient food and water available in the market. Discuss with dealers.
  • asked a question related to Infertility
Question
5 answers
Bovine infertility 
Relevant answer
Answer
Hi,
From my experience supplementation of minerals to infertil dairy cows does only make sense, if you have a proven deficiency - in German herds a rare situation.
Clinical examination should be done to find the reason for the poor BCS. If parasits (fluke e.g.) and other individual diseases can be excluded , the majority of cases you discribed occurs in consequence of subclinical ketosis during a period of time within the first 6 - 8 weeks after parturition.
With regards
  • asked a question related to Infertility
Question
7 answers
what are the species that you are going to use
Relevant answer
Yes definitely and as a collaborator after negotiations through institutes :)
  • asked a question related to Infertility
Question
4 answers
A diabetic patient either type I or type II  is on regular medication and has kept his glucose levels in normal condition will he become infertile. And if he becomes infertile can we say that it is due to diabetes.
Please explain
Relevant answer
Answer
Optimal glycaemic control is crucial to sucessful management of diabetes mellitus, however, we should not forget that diabetes is a chronic disease that runs its natural course; good control of blood sugar only slows it down. Therefore,it is possible that reduced fertility can still complicate the disease despite an apparently good glycaemic control.
  • asked a question related to Infertility
Question
3 answers
   I am now working on the Co-immunoprecapitation of human sperm. I have tried some lysis buffers such as RAPI, lysis buffer purchased from Thermo and standard IP lysis (50mM tris, 150mM NaCl, PH 7.4). However, the lysate concentrations were very low. Is there the protocol or usable lysis buffer in human sperm? Thank you for your kind suggestion.
Relevant answer
Answer
Hi, Hao
I think with 500ug of protein (independently of the volume) you would be able to perform an IP. I use 200-400ug for IP with good results.
Your lysis buffer is not the problem, sperms always give low protein concentration.
Why do you use so many lysis buffer and incubate for so long at 4ºC (2 hours)?
Maybe you should try to perform the IP anyway.
Which protein do you want to observe? It is a low-expressed protein?
  • asked a question related to Infertility
Question
2 answers
I have been using endothelial cells with various conc of glucose from 1.5mM to 30mM. I have changes in total AMPK protein expression using AMPK alpha1/2 santa cruz. I just went through some literature which states the subunits may have changes in expression. Please help me to clarify myself that yes/no Total AMPk changes
Relevant answer
Answer
Thank you Go J Yoshida for the information. I would go through your publications if in case of any doubts would contact you ..
  • asked a question related to Infertility
Question
2 answers
24-years-old came for fertility consultation. She is obese (75 Kgs), Primary Amenorrhoea, Karyotype 46XX/47XXX (50%/50%) and streak ovaries on laparoscopy. She has almost all features of triple X syndrome. FSH 76, LH 25, AMH 0.6 (low fertility), estradiol 36pg/ml (Premenopausal range). she bleeds on withdrawal with MPA or Ovral-L.
Someone has advised her IVF-ET.
My question is that can she be tried for IVF with so high FSH and does she need physiological sex steroid replacement till 45 years of age (even when she has fair amount of estrogen production)?
Relevant answer
Answer
When you karyotyped her what tissue did you use?  blood, I presume.  It could be useful to determine the extent of her mosaicism -is it also in ectoderm? Karyotypng from a fibroblast biopsy may be useful or even FISH on hair follicles.  
  • asked a question related to Infertility
Question
6 answers
Dear All
Are there any Available Dataset to download for Infertility Cases { IUI or IVF Method } ?
I Found Some Datasets For Cancer or Diabetes but I cant Find any dataset for Infertility cases!
Best Regards,
Ehsan
Relevant answer
  • asked a question related to Infertility
Question
5 answers
MTHFR mutation 677 and 1298 has relationship with so many diseases including miscarriage, infertility, neural baby defects. I understand folic acid should not work in this case. does l-methylfolate work better than folic acid? 
Relevant answer
Answer
Definetly L-methylfolate
  • asked a question related to Infertility
Question
6 answers
Is there any survey or research world wide that reflects co relation of vitamin D deficiency and number of children?
Relevant answer
Answer
In men, vitamin D is positively associated with semen quality and androgen status. Moreover, vitamin D treatment might increase testosterone levels. Testiculopathic men show low CYP21R expression, low 25(OH)D levels, and osteoporosis despite normal testosterone levels.Vitamin D and fertility: a systematic review – May 2012 
Eur J Endocrinol May 1, 2012 166 765-778
  • asked a question related to Infertility
Question
13 answers
How can you predict and prevent infertile women who undergo IVF of having ovarian hyperstimulation syndrome after IVF?
Relevant answer
Answer
Regarding prevent ovarian hyperstimulation syndrome, think about ORPI (ovarian response prediction index)
Reprod Biol Endocrinol. 2012 Nov 21;10:94. doi: 10.1186/1477-7827-10-94
  • asked a question related to Infertility
Question
3 answers
I want to know any editors interested in this issue.
Relevant answer
Answer
Dear Pavel:
         Thank you for your answer. But we use the new long effect of ovulation drug(corifollitropin-α ,Elonva) for COH. We found the incidence of premature LH surge(>10 IU/L) can be decreased to 2.4% if  use antagonist when LH >6 IU/L.
          Previous studies have reported premature LH surge during the administration of the antagonist in rates up to 8% and in some cases up to 28% (Borm and Mannaerts, 2000; Felberbaum et al., 2000; Engel et al., 2002).  Why not inject the antogonist  before LH surge ?
      Best regards
      Meng
  • asked a question related to Infertility
Question
4 answers
My master thesis is about infertlity, I want to khow about any psychologically and personality traits differences among them, And I want to make a questionnare for causes that infertile people believe that their infertility is came from that causes. I need some information about infertility perception.
Kind Regards
Relevant answer
Answer
This is a very interesting topic as it is a bit of a conundrum. The questions to answer are: Does fertility affect personality or does personality affect fertility; and also how much does lifestyle impact fertility. Perhaps you can look at the lifestyle factors that impact fertility and subsequently what the personality traits are of those people displaying these typical lifestyle factors.
  • asked a question related to Infertility
Question
3 answers
Is there any role for metroplasty?
Relevant answer
Answer
Not any more. Data had shown it does not increase pregnancy rate. I used to do the old Starmann operation (i think that is the name). Tubal blocking was not uncomen
  • asked a question related to Infertility
Question
2 answers
Over-proliferation of spermatogonia caused infertility in male flies, so I am wondering whether similar phenomenon can be seen in mice/human spermatogenesis.
Relevant answer
Answer
Thank you for your comment. I will look into those papers.
  • asked a question related to Infertility
Question
7 answers
The loss of the cremasteric reflex after hernia repair is usually considered as a sign of the ipsilateral testis non-functioning. Are there any data of the correlation of the former and the latter?
Relevant answer
Answer
I think the cremasteric reflex is limited to childhood, perhaps correlated to differencial diagnosis of retractil testis till the age of about 2yrs.
  • asked a question related to Infertility
Question
1 answer
This is a cross match test to determine the presence of anti- lymphocyte antibody in female partners suffering from immune mediated infertility, Like that developed by Dr Alan Beer and currently offered in his Lab and Rosalind Franklin Lab, Chicago.  Would also be used in transplantation studies  
Thank you
Relevant answer
Answer
I believe there are kits for this.  The American Red Cross  (ARC)Specialty lab does this, as well as NIH. These are micro well plates.  These are serological tests.  I would contact ARC in S. California (formerly Dr. Garratty's lab). Dr. Garraty passed last year. There they do flow cytometry crossmatches.  They would be the first I would talk to. 
Mixed lymphocyte studies is a pretty old method to do this, but it actually works well.  This would be for cellular immunity.
Hope this helps 
Sergio
  • asked a question related to Infertility
Question
3 answers
I want to work on autosomal recessive infertility in humans.
Relevant answer
Answer
Dear Ansa,
There are hundreds of genes influencing the male as well the female fertility. In the male the most of these genes influence the spermatogenesis and/or the sperm function (motility, acrosome reaction, sperm egg interaction, sperm oocyte activation etc.). In the female there are the genes influencing the ovarian function, the endometriosis predisposition, the aneuploidy predisposition, the endometrium receptivity etc. Besides, the lethal mutations of many genes may play a role if they are in the homozygote combination.  In my opinion, your topic is very interesting, but it will be a huge work, I suppose to select a part of this topic.
Best regards
Pavel Travnik
  • asked a question related to Infertility
Question
3 answers
Or a semi-structured interview.
Relevant answer
Answer
Buongiorno professore,
mi permetto di risponderle in italiano. Anche secondo me il counselling è più appropriato della terapia. Nonostante ciò, mi piacerebbe esplorare l'efficacia di un counselling di coppia. Leggendo gli studi precedenti, ho notato che la "marital congruence" è una variabile molto importante per il superamento dei vissuti negativi relativi a questa situazione, e questa si esplica solo a livello di coppia (che io sappia). Sono solo uno studente al primo anno della laurea magistrale in Psicologia Clinica, quindi non so ancora molto dell'argomento; tuttavia, ho intenzione di realizzare un "qualcosa" per la mia tesi (test, intervista o altro ancora) utile per il clinico nella fase di assessment. Il clinico non credo che sia consapevole delle numerose variabili che sono in gioco e che influenzano i vissuti psicologici della coppia che si sottopone a PMA; con il mio lavoro vorrei renderlo consapevole e indirizzarlo verso una corretta "diagnosi".
  • asked a question related to Infertility
Question
9 answers
I am a pathologist and want to start using advanced sperm function testing in cases of infertility. I am interested in Mitochondrial membrane potential. Is it a good assay to use routinely in cases of male infertility? 
Relevant answer
Answer
Due relevance of mitochondrion on sperm physiology, I think that evaluation of MMP is suitable to be included when is evaluated any case of male infertility. Although I don't know any reported case of male infertility associated with diminished mitochondrial function in domestic animals, in our laboratory and others at world, the estimation of mitochondrial function is included in a battery of tests to determinante the fertility potential of male.
  • asked a question related to Infertility
Question
1 answer
During artificial Insemination in Honeybee queens, someone uses chloroform to help endophallus eversion process in drones. Does anybody know if there is any paper concerning chloroform affecting sperm vitality (in any species.. not honeybee only)?
Relevant answer
Answer
sorry I dont know
  • asked a question related to Infertility
Question
3 answers
I have to know the basic cause of the infertility in closely related families to proceed my research further.
Relevant answer
Answer
One of the cause of lower fertility in consanguineous couples is common HLA halotypes but conversely it is also reported that consanguineous marriages have higher fertility rates because of greater genetic compatibility between mother and developing fetus. 
  • asked a question related to Infertility
Question
4 answers
I am studying the Epidemiology of Infertility in the MENA region.
Relevant answer
Answer
You can also design a good protocol by making specific heads for each type of problem of infertility found in females and males.
(1) Name of infertile 2) sex,3) age), 4. livelihood index  5. Pedigree  6, Symptoms
7. Gamate count, 8. Gamete persistence 9. Presence of sperms/ml of semen or in oggonial secretions 10. mensuration failures 11. reasons 12 diagnosis required and done 13. Group specific reasons 14. eco-climatic region 15. Social evils/no evil or addiction 16. length of sex life passed  
  • asked a question related to Infertility
Question
8 answers
Varicocele is a quit common disease. Surgery is done for many indications as infertility, annoying pain, or complications. Many approaches for varicoclectomy are present, which approach you think is better, Does it differ according the the indication of surgery?
Relevant answer
Answer
Laparoscopic Vericocelectomy is an easy procedure with good results.  
  • asked a question related to Infertility
Question
6 answers
prolactin level is normal
Relevant answer
Answer
Elevated levels of prolactin inhibit the pulsatile secretion of GnRH, and that can cause severe oligospemia in men, thus presenting with hypogonadism and infertility. In such situations, dopamine agonists are indicated for the treatment of infertility and the pituitary tumor. Both bromocriptine and cabergoline can be used. Nevertheless, cabergoline is more efficient than bromocriptine in cutting prolactin production (Webster J et al 1992), and has been shown to normalize prolactin levels in 70% of bromocriptine-resistant patients Verhelst J et al 1999). Consequently, cabergoline (0.125-1.0 mg twice weekly) is the favored option, as it has the utmost efficacy in regulating prolactin levels and attenuating prolactin-secreting tumors
Webster J, Piscitelli G, Polli A, et al. Dose-dependent suppression of serum prolactin by cabergoline in hyperprolactinaemia: a placebo controlled, double blind, multicentre study. European Multicentre Cabergoline Dose-finding Study Group. Clin Endocrinol (Oxf) 1992;37:534-41.
Verhelst J, Abs R, Maiter D, et al. Cabergoline in the treatment of hyperprolactinemia: a study in 455 patients. J Clin Endocrinol Metab 1999;84:2518-22.
  • asked a question related to Infertility
Question
3 answers
I would like to review some articles / projects about infertility in oncology.
Relevant answer
Answer
Probably this recent paper is very appropriate in your research... have a look:
J Oncol Pract. 2014 Dec 30. pii: JOP.2014.000786. [Epub ahead of print]
If You Did Not Document It, It Did Not Happen: Rates of Documentation of Discussion of Infertility Risk in Adolescent and Young Adult Oncology Patients' Medical Records.
Quinn GP1, Block RG2, Clayman ML2, Kelvin J2, Arvey SR2, Lee JH2, Reinecke J2, Sehovic I2, Jacobsen PB2, Reed D2, Gonzalez L2, Vadaparampil ST2, Laronga C2, Lee MC2, Pow-Sang J2, Eggly S2, Franklin A2, Shah B2, Fulp WJ2, Hayes-Lattin B2.
  • asked a question related to Infertility
Question
9 answers
Does thrombophillia have a significant effect on implantation failure and recurrent IVF failure?
Relevant answer
Answer
Clearly thrombophilia brings repeat abortions and  constitute a major cause of infertility by promoting hypercoagulability of placental vessels.  Whether it is genetic and acquired its preventive treatment consists of administering aspirin or heparin in early pregnancy, maintaining treatment until delivery. Current is forced to study mutations in coagulation factors (at least II, V, XII) and MTHFR. Safdarian et al)(Iran J Reprod Med. 2014 Jul;12(7):467-70) found that mutations for factor V and homozygosity for MTHFR are risk factors for IVF faillure . Detachment of placenta, preeclampsia, hypertension or intrauterine growth retardation are other possible complications of pregnancy induced by thrombophilia.
  • asked a question related to Infertility
Question
15 answers
pt' with necrozoospermia ( 100% immotile spermatozoa) , how we can get fertilization by ICSI?
Relevant answer
Answer
Agree with Prof. Tulay Irez:
Use HOS Test to identify sperm with intact normal DNA.
Those sperm exhibiting tail curling can be used for ICSI, we believe.
However, such a study should be reported.
Ref: Stanger et al, 2010. RBM Online 21, 474-484.
Best wishes and do report your experience.
John Yovich
  • asked a question related to Infertility
Question
15 answers
Some of our researchers believe that consanguinity is one of the causative agent of human infertility. Is it possible or not? If the consanguinity is the causative agent, then what is the reason behind that? What kind of changes take place due to consanguinity?  
Relevant answer
Answer
Yes it is possible. In fact, the main impact of consanguinity is an increase in the rate of homozygotes for autosomal recessive genetic disorders including (at least) some particular and rare genetic forms of male infertility.
  • asked a question related to Infertility
Question
4 answers
Thanks in advance.
Relevant answer
Answer
The paper about the CMV & HSV being positive in the sample of the sperm is a very authentic evidence that causes the anatomical anomaly in the spermatoneogenesis, including but not limited to the aspermia. If you simply check your patients for the antibodies (IgG) present in the blood over the cut off value, that will lead you to determine the root cause of the aspermia, oligospermia, etc.
What we traced was as following.
1. The Neurons responsible of secreting the "Neuro Secretory Enzymes" (NSE) are inflicted with the CMV RNA proliferation, therefore, secreted with the NSE to stimulate the Pituitary. The molecular integrity of the NSE is compromised.
2. The pituitary stimulation by the compromised NSE in turn gives rise to the compromised stimulating hormones, including FSH and others.
3. Comes the arrival of the Neuro viral RNA into the testes along with the stimulating hormone.
4. A variety of sperm reproductive anomalies take place.
Best Regards.
Prof. Shahid H. Sheikh,
  • asked a question related to Infertility
Question
4 answers
Stem cells transplant in primary testicular failure.
Relevant answer
Answer
The paper about the CMV & HSV being positive in the sample of the sperm is a very authentic evidence that causes the anatomical anomaly in the spermatoneogenesis, including but not limited to the aspermia. If you simply check your patients for the antibodies (IgG) present in the blood over the cut off value, that will lead you to determine the root cause of the aspermia, oligospermia, etc.
What we traced was as following.
1. The Neurons responsible of secreting the "Neuro Secretory Enzymes" (NSE) are inflicted with the CMV RNA proliferation, therefore, secreted with the NSE to stimulate the Pituitary. The molecular integrity of the NSE is compromised.
2. The pituitary stimulation by the compromised NSE in turn gives rise to the compromised stimulating hormones, including FSH and others.
3. Comes the arrival of the Neuro viral RNA into the testes along with the stimulating hormone.
4. A variety of sperm reproductive anomalies take place.
Best Regards.
Prof. Shahid H. Sheikh,
  • asked a question related to Infertility
Question
9 answers
Obese female diabetic in reproductive age anticipating pregnancy.
Relevant answer
Answer
what results are you getting vis a vis metabolic labs with the combination of Topiramate and naltrexone? Insulin? glucose? estradiol? LH? FSH? leptin?