Industrial Biotechnology - Science topic

Dear Researchers,

I am writing to inquire whether any evidence exists for the industrial-scale production of specialized/ secondary metabolites using Nicotiana benthamiana as a platform.

Thank you,

Nuwan Sameera Liyanage

Years back, I have taken notes from that book for my ug assignment, but I missed to note down the author’s name.

Now, I need the book for further reference. I searched a lot but i could not find it.

I could not get the book without knowing the author’s name.

BOOK DETAILS

TITLE: TEXTBOOK OF MICROBIOLOGY

CHAPTER 29: TOOLS OF FERMENTATION TECHNOLOGY ON PAGE 1021

If anyone knows the author details and the edition of the book, please share it.

Thank you in advance.

I would like to produce gel filament of cell entrapped in polymer solution. But when I mix the suspension with the polymer solution, a pre-gel is formed and air bubbles are also entrapped in the mixture. I've already tried to removed the air bubbles by vacuuming but it takes very long time and isn't good for cell viability. Any idea how to remove the air bubbles without causing cell damaging?

for my university project, I need a mutant Brevibacterium flavum for L-glutamine manufacturing. but I don't know which from where I can buy it.

which institute sell that?

If we consider, for example, heterologous insulin biosynthesis in E. coli, we often find that a strategy based on synthesizing the target protein by expression plasmids has simply been adopted. But wouldn't that be a problem in terms of plasmid stability during continuous production on an industrial scale? Shouldn't such operations be performed with modifications at the chromosome level? There are definitely different nuances in other hormones, enzymes and proteins, thus I would appreciate it if you could explain by giving some examples.

Thanks in advance..

Hi, all. I am now scaling up a GS-CHO cell culture process from lab scale to pilot scale for the first time. I don't know what will happened and what should I adjust. What problems have you encountered and how have you solved them when you scaled up a CHO cell culture process from 1L or 5L stired tank bioreactors to 200L,2000L or larger?such as metabolic flux shift? viable cell density decreased faster? peak viable cell density was higher but titer was low?

In your opinion, what plant breeding plant can be important and useful in the future?

How can we produce standard and uniform BC in sheet form, especially with low costs and cheap carbon sources?

I am looking for available databases for a standardized list of SOPs. More specifically, I am looking for a standardized SOP for anthocyanin extraction from deepwater rice. This SOP must be for industrial use.

The dose is 2.8 grams and I'm wondering what the production costs of that might be

to get idea on different methods to develop inoculum for anaerobic digestion process.

want to know there is any strategy for this process.

Biochemistry

Biotechnology

Bioprocess

According to the pathway by which the metabolite produced can be classified to one of the three types :

1-fully growth associated metabolites,

2-partially growth associated metabolites,

3-non growth associated metabolites

What type of flocculants are used to clarify sugar juice in organic sugar production? At least in Colombia, we only know those extracted from plants.

I am trying to determine the degree of polymerization for bacterial cellulose but I can't find the complete method. Can anyone suggest the suitable method for this test?

I am working on the sorbitol production by fermentation. So after the end of the each optimisation step, I need to analyse the Sorbitol, for that the ONLY available method to quantification is HPLC.

Hi, I'm looking to produce a highest biomass production of Saccharomyces cerevisiae in the Laboratory, in a stirred tank bioreactor (with working volume of 3 L), for this purpose, I'm trying to choose the best strain to fulfill my goal.

I appreciate the answers.

I'm trying to prepare SDS solutions in NaOH 0.5M in concentrations ranging from 0.1 to 0.5% at room temperature. All the solutions appear to be insoluble unless heated to >25°C. Moreover, the day after, all the solutions have a visible precipitate (the temperature overnight being around 21°C). I've tried different preparation methods (powder SDS in liquid NaOH, dilution of two stock solutions, powder SDS + NaOH in pellets in water etc.), but I get more or less the same results. I get the same effect (less pronunced) with SDS in water only. However, I've found material a data sheet where a solubility of about 150 g/L at 20°C is given. Can anyone help?

If I have multiple points where it is linear, should I use all the points that are linear to account for the calculation of specific growth rate?

Also, how linear should the line be? what are the parameters of the linearity? Is it too strict to use a R(squared) of equal to 0.99 and above as measure for linearity?

I'm working on protein production in P. pastoris, but the protein looks toxic to P. pastoris because yeast get dead after induction by methanol. How can I produce(express) protein which have toxic effect to yeast without changing host? Should I use any synthetic promoter or other chemicals? 

Do you have experience on the effect of YE from different suppliers on fermentation performance (i.e. max biomass and product concentration)?

How can I get saccharomyces cerevisiae strain?

Dear all,

I would like to know if a regular off-the-shelf baking yeast can be used for laboratory scale and cultured on minimal media without amino acids or vitamins as i want to grow them on 13C-glucose to end up with fully labelled metabolites that could be used as internal standards for quantitative analysis of lipids in other biological samples . and if not, can anyone suggest a suitable strain that will grow on minimal media with one carbon source.

Any pointers given are much appreciated. Thank you!

Looking at CDC28s role within the cell cycle of s. cerevisiae and a diagram displaying its more essential cell cycle functions would be really useful as a clear illustration, rather than a large wall of text. 

Hi,

I am working on Carbon-dioxide sequestration by microalgae using coal-fired actual flue gas in photobioreactors. The flue gas is being used as it is (without any pre-treatment).  I am studying the flue gas introduced heavy metals into the system (medium and/or biomass). Could anyone please throw some light, which heavy metals I should be focusing on when analyzing the samples in ICP-MS. I am looking for Ni, Cr, Cd, As,Pb but Mo,Sn, Rb are also seen. Is it a fine observation? 

Thanks

Geetanjali

I found that for different media different sterilization SOP follows. Some passes the pure steam into the media after reaching temperature 95 or 90 through sparger and close the jacket steam and some not passes the steam inside the media and close the exhaust valve after reaching the temperature 95 and continue the sterilization with jacket steam.

which is best?

Is there some particular Actinomycetes strains that are particularly interesting/ better suited for industrial biotechnology applications?

Shake flasks are predominant platform to culture the microbial cells. Precise measurement helps to add external oxygen to provide oxygen enriched environment that will increase the growth of cells and recombinant protein production.

I m working on kinetic study of biodiesel production. my questions are as follows

1. I want to analyse the sample with HPLC so which concentration should i estimate, is it Triglyceride or FAME.

2. what are the standards generally used for the estimation for both TG and FAME.

3. After getting the concentrations how to estimate the rate constant.

4. can we estimate the gibbs free energy from calculated rate constant.

Hi,

If my solved structure is a dimer in an asymmetric unit, are the two molecules correlated by crystallographic rotational symmetry?

I think not, but have problem explaining it. I would be grateful for some help.

Thanks

I am looking for new technologies on citric Acid productios, speacially for fermentation and purification process

I am going to clone and express a glucoamylase gene in an auxotrophic Saccharomyces cerevisiae using YEp 352 which has a LEU2 and URA3 selective marker, in order to produce a one-step conversion route for ethanol production, therefore a strain which has a strong capability to convert starch to ethanol is much needed. thanks. 

I am trying to calculate the specific growth rate for a species of Acetobacter. The substrate is glucose. Batch culture is used by regulating pH, temperature, and dissolved oxygen.

According to some textbooks, the specific growth rate is the highest during Log phase. However in my calculation, the specific growth rate is the highest just after lag phase. A biomass-OD curve was used to determine biomass during the growth phase.

How can I interpret these results?

I'm a student that do a project by using yeast for recombinant protein production, and I'm out of this field for cell-free protein production, but I have some doubt.

I see many kits from many company that use very short time for 30 minutes or 2 hours for protein production that very convenient. If compare with microorganism that use a longer time for day or many days. And microorganism have to construct the plasmid that have many elements that affect the expression level. Have to choose promoter, terminator, selection marker, culture medium, the induction or cultivation system, optimization for protein in flask culture or bioreactor etc. Therefore, I see many disadvantages for use microorganism for recombinant protein production.

So, for many time that I think about this question. Why In Vitro (Cell-free) recombinant protein synthesis don't use instead of microorganisms in the industrial scale? May be a price? or any reasons please suggest me.

Thank you so much,

Tatpong

I need to know if Laccase has achieved the level of being an industrial enzyme or is still a potential for future? If yes, I need to find somewhere that is using laccase for this purpose. Any company, or any process with laccase. 

Dear Colleagues..

I would like to invite you to contribute a book chapters

=> The preliminary acceptance is only upon submission of the draft abstract.

=> The book will be possibly published in second quarter of 2016.

=> There is no processing/publication fee

=> There are no page charges for black and white figures and illustrations submissions provided they are sent to Elsevier with the source files. Color illustrations are billed at the current rate.

=> An author guidelines will be sent to all authors after acceptance of the abstracts

=> In consideration of your contribution to this book, Elsevier will provide to all authors an electronic edition of the book, besides giving a 30% discount on all Elsevier Science books for life.

Please text me within 2 weeks if you are interested.

Thanks

GS Dhillon, PhD., P.Biol (ASPB)

I'm trying to grow halobacterium salinarum (ATCC 19700 strain) in hopes of collecting and purifying purple membrane. Out of this I will further purify bacteriorhodopsin using size exclusion chromatography.

My questions is this: why is my culture pink, not purple? I've grown it in the recommended media (ATCC 213), under aeration (250rpm) in shaking flasks (500mL in 2L flask), and with a continual light source at 37C. These archaeal cells should be purple, implying expression of bacteriorhodopsin, but instead are pinkish/orange.

If anyone could help, I would appreciate it, my supervisor is demanding this protein ASAP.

I am providing oxygen through sparging to my system (fermented broth) and it increases drastically the DO level in the broth. I am doing also agitation  in the system for breaking of the large molecules of air bubbles, formed due to sparging, hence for proper mixing. But without sparging, the agitator system individually  does not help in increasing the DO level in the broth.

I have checked it for lower to higher agitation speed maintaining the time of oxidation constant and no aeration,but still the DO is not increasing.

What does it mean?

Is that my limitation of agitator system?

Thanks for your input!

I'm helping in a project that aims to produce and immobilize the laccase enzyme from P. ostreatus, but we have not got a good immobilization protocol yet ( adequate concentration of glutaraldehyde and good response surface). Perhaps anybody who has done this kind of immobilization by CLEAS?

I'm looking for some help in setting up a Bioreactor system for cultures of Cupriavidus necator which will be gas-fed growths. My problem comes in determining the flow rates that will be required for the gases. Without some idea of the necessary flow rates suitable to feed a culture, I cannot determine which Mass Flow Controller's I will need to purchase.

So far my search for information has only revealed various ratios of gases used previously, and that the rates must be varied in order to support the growths. What I need to know is the ratio of gas flow to culture size, so I can calculate the range of flow control I will need for my system in relation to the vessel size, for Air, Oxygen, Nitrogen and Carbon dioxide.

Does anyone know this information? Or can direct me to some papers/journals/books etc that can give me some idea? Or even have experience in Cupriavidus necator cultures?

Any assistance will be very welcome. Thank you.

thank you

I am trying to explain an industrial production of bacterial consortium for the degradation of azo dyes but is difficult because is like an industrial secret. So i would like to know about the size, composition and downstream of a bioreactor for the production of a consortium, could be for Bacillus species and Pseudomonas.

I am very interested in the field of hydrometallurgy and decided developed a manufacturing plant copper by hydrometallurgy method. However, due to the use of sulfide ores (Cu-Fe-S) we are forced use of bioleaching technology. Does the subject written in the articles for procedures for hydrometallurgy, is this done on an industrial scale?

I wanna check the ability of one bacteria to produce chitinase enzyme, in the literature it showed some methods to obtain the colloidal chitin, but last time I tried it, it failed, maybe because of the filtration step or some other reasons, if someone have experience with that, what could be an easy way for preparing colloidal chitin ?

I want to know about the highest Open circuit voltage achieved so for in single chamber air cathode MFC?

The reactor is a batch reactor having agitator and sparger system attached.I am working on Indigo dye formation without involvement of any micro-organism .The product formation is merely a chemical conversion in presence of oxygen. I want to optimize the effect of aeration,agitation and oxidation duration on dye formation as well as their kinetics.

Kindly help me on this

I would like to know which are the most important points I should consider when performing mRNA extraction from bacteria.

Though there are several nutrient sources for fungal cellulase production at bench/lab scale, I am interested to know about those carbon and nitrogen sources as well as cellulase inducers that are actually used or are economically feasible for commercial scale production of cellulase enzyme using Penicillium and Trichoderma.

Bacillus subtilis used for the production of enzyme by solid state fermentation.

Any author will be fine

I am working on polylactic acid production from lactose. After production of lactic acid, is there any biological (microbes/bioenzymes) which can convert it to polylactic acid? Direct conversion of lactose to polylactic acid by microbes is also desirable.

the pattern of consumption of amino acid is depend on yeast strain. 

but is there any one knows the availability of oxygen also cause to consumed more amino acid or not?

Which part of these are more advantages in removing hazardous chemicals from wastewater?

Itaconic acid is produced through fermentation process by Aspergillus sp. at pH 3. This species use Kreb cycle intermediate for the production of IA. now the question in my mind is the role of itaconic acid in fungus.. Why the Aspergillus sp is producing IA.. Is IA a primary or secondary metabolite??

VSD with low temperature treatment 40-60C.

I perform DSC,  temperature  -60 to 120C, of my spray dried powder sample. But i cant set the base line which transition 1 or 2  will be the glass transition temperature Tg. When i set to base line i got onset, endset and midpoint temperature. Which one is the Tg. In Gordon Taylor model,  Tg, Tgs, and Tgware the glass transition temperatures of the mixture, solids, and water, respectively,xw is the mass fraction of water, and k is the Gordon-Taylor parameter, how can i predict. specially k value . my sample solid about 96% water 4%. 

I deal with production of Lipase from Candida sps. (CALB). The fermentation has been recently scaled up to a 3L fermentor. The problem that I face now is the fluctuating O.D values of the broth measured through a period of 48 hours. I cannot seem to point out the actual cause of the decline in O.D. 

Hi all,

May I know what is the role of acids (HCl, H2SO4, Acetic acid, phosphoric acid, Oxalic acid and nitric acid) in removing lignin/ cellulose/ hemicellulose in lignocellulosic biomass?

If we use HCl what it will do for Lignocellulosic biomass

If we use H2SO4 what it will do for Lignocellulosic biomass

If we use Acetic acid what it will do for Lignocellulosic biomass

If we use Phosphoric acid what it will do for Lignocellulosic biomass

If we use Oxalic acid what it will do for Lignocellulosic biomass

If we use above acids for steam pretreated biomass what will happen

and 

If we use sodium hypochlorate what it will do for Lignocellulosic biomass

If we use sodium sulphite what it will do for Lignocellulosic biomass

Please let me know in detail

Thank you

If I want to prove that the half-life of my mRNA is low, what experiments should I do? I have the q-PCR data, can I determine the half-life from the data?

Industrial symbiosis is the sharing of services, utility, and by-product resources among industries in order to add value, reduce costs and improve the environment. Industrial symbiosis is a subset of industrial ecology, with a particular focus on material and energy exchange.

I'm working with manganese-iron oxidizing bacteria

I have yeast like isolates that grow on avicel, yet not producing enzyme extracellularly. i.e. I cannot detect hydrolytic effect on agar plate using Congo red/Dyed substrate.

I am trying to isolate the SlpA (Surface Layer Protein A) protein from Lactobacillus brevis with the Laemmli cell lysis buffer. I am still unable to get the precise protein band in SDS PAGE run at the 44.31 KDa. Please suggest any specific method to isolate the cell surface proteins. And also suggest the protein storage method for a long time.

it always forms two layers ,due to polar and non polar properties, so fatty acids are not properly transfer in to the medium ,some one would suggest me to solve the problem

I am working on itaconic acid production through fermentation of sugars. I have to separate the itaconic acid from the fermentation media through crystallization technique. As both the itaconic acid and sugars are water soluble, which compound will crystallize first? Or how I purify the itaconic acid from sugars?

I need to extract this compounds from my biodiesel sample in order to characterize them in HPLC tests. Any suggestions are welcome.

Problems estimating the activity of tyrosinase from a culture supernatant? Is there any medium to suggest? Detailed protocol for tyrosinase assay would be great.

In my current project, I am dealing with bacterial strains collected from an abandoned gold mine. I want to determine the amount of cyanide produced by these isolates.

I have done the qualitative analysis experiment which confirms the ability of the studied isolates to produce cyanide (by change in color of filter papers).

But I want to know how to estimate the amount quantitatively since I don't have any specific kits or electrodes to do so.

In one article, the use of Kings B agar was mentioned (the lids of petri plates were fitted with Whatman No. 1 filter papers) which after incubation (48 hrs, 28 deg. C temperature) has to be extracted with 5 ml, 1 M NaOH and then titrated with 4.25 ml acetic acid. After that, the cyanide in NaOH solution was quantified by absorbance spectrophotometry at 575 nm after allowing the NaOH solution to react with barbituric acid-pyridine reagent.

My question is, how do I get the quantitative value after having the absorbance readings at 575 nm? What would be the concentration, or unit of the produced cyanide?

Thanks beforehand for your time on this and for your valuable contributions as well towards my question.

I have been doing research in Industrial Biotechnology. My research is about how to improve and enhance pigment production from Fungi (Monascus sp and Gibberella sp.) by co-culture fermentation with some actinomycetes. The goal of the research is to get the optimum yield and analyze the potential application of the pigment as natural dye in textile industry (e.g. as the cotton dye). I need somebody who can share ideas with me and discuss this.

My shake flask experiment when lactobacillus delbreuckii lactis ATCC4797 grown in media containing lactose and casein hydrolysate, in which trace quantities of calcium carbonate added as neutralizing agent. We observed increase in pH from 6.72 to 8.73 after 46 hours. Cell OD is also found to increasing ironically. I would like to put before this forum to what would be the possible cause of increasing pH whereas the with the same organism and media components without calcium carbonate were showing decreased pH as is expected.

Conventional enzyme screening methods are often time consuming and laborious. There must be some alternative way to do this. Can anybody suggest a reliable method for this?

I am writing to ask about a problem that has been happened in cultivation of a microbial strain, lactobacillus Bulgaricus.

I used to culture this microorganism in a 10litere bioreactor and reach a certain optical density but after my previous working banks finished, I made another series of working bank from the same mother stock. These new working banks are not as efficient as my previous working bank. Although the inoculums which are prepared by culturing the working bank growth well as usual, the final optical density in bioreactor has decrease to a quarter. For finding the reason of the problem I repeated this procedure twice and the result was the same. Even using direct inoculums from another mother bank that had not been thawed before, could not solve this problem and the final optical density was not as high as before. The fermentation conditions and medium ingredients were the same throughout all the experiments. I am looking for the reason of this problem and want to find out what was happened to these cells.

In this study we evaluated the optimal physical requirements such as the shaking rate and incubation time for applying in liquid media malt extract broth to obtain a high emestrin compound and its toxicity.

Fungi, heavy metals

The objective is to produce small peptides (15 amino acids maximum) by an hydrolysis. Is the method of Biuret a good approximation? Or it is better to use a gel?

Over the long term, life sciences will provide the basis for substantial numbers of improved jobs and economic progress. Biotechnology provides economic value over the shorter term and is more immediately obvious to people and governments. Should we be developing programs that collectively and efficiently achieve both deep science and efficient technological applications? This is not the same as translational research, as it is more about deep research and about applying resulting technologies to appropriate problems.

What is the most suitable microorganism? Also, what is the value of the yield? I am going to work in the lab with whey to produce lactic acid in the fermenter, but I don't know how to start.

We are conducting a design project where the production of Amevive is our topic. We realise that it was subsequently taken off the market due to it's ill effects on the patient. However, any help on processes developed circa 2004/2005 would be appreciated.

I am looking for a treatment which eliminates Lipopolysaccharide (endotoxins) in an Exopolysaccharide production from Gram negative bacteria.

.