Questions related to Inclusion
I use the following method to wash my inclusion bodies: 8 steps.
1: Troton, EDTA, Tris and Fresh DTT (3 Times)
2: Doc, EDTA, Tris and Fresh DTT (3 Times)
I use this method for 3 different recombinant proteins. In 2 of them, host proteins were removed up to 90% during washing. But in the third case, a very small amount of host protein is removed and I have a lot of impurities.
Can someone tell me that
Do I need to change the concentrations of the compounds in the washing buffers?
Do I need to choose another method?
Choosing an IB washing method depends on what factors?
Thanks a lot.
In her ECPR essay for the sciences of the democracies, Jenny Wustenberg argues that memory offers training to citizens, especially in the arts of tolerance and inclusion.
Whilst this point may seem, or be taken to mean, something obvious - it isn't. In fact, as Wustenberg points out, the concept of "mnemonic democracy" is (a) barely used (it came up with a few hits on a general Google search, for example) and it (b) also stumped Chat GTP which usually comes up with something for any question!
Given the above, I think Wustenberg is right to argue that there is a lack of focus on the intersection between memory and democracy. We need to focus more on how "actors engage in negotiations over public memory. For all of them, the power to define what a democratic approach to the past means is highly valuable."
What do you think?
We are currently conducting a qualitative meta-analysis and looking for qualitative research that is focused on transgender, non-binary, and gender diverse people’s experiences of gender identity development and how their gender functions to support coping with minority stress as well as increasing resilience and flourishing. We are looking specifically at articles that focus on participants in the United States. We are contacting scholars to ask if they have authored or are aware of studies that may meet our inclusion criteria, particularly any new or unpublished studies. Our team will screen the articles to determine if they meet our inclusion criteria. If you know of any studies that may be relevant to our qualitative meta-analysis, please contact or send them to Kelsey Kehoe at email@example.com.
Heidi Levitt & Kelsey Kehoe
I am researching on a topic where I intend to use both qualitative and quantitative data.
In my qualitative data, I am employing purposive sampling for participant selection but I keep getting stuck in the area of what would be my inclusion criterial
Hello , After I have affinity (6x his pet24) purified protein from inclusion body using 50mM tris ph8 , 300mM Nacl , 250mM imidazole and 8M urea I have subjected it to dialysis in 50mM tris pH 7.6 and 150mM Nacl. Initially after 1 to 2 hours of dialysis at 4 degree C I saw some whitish precipitate in the bag. I took samples at 0hr ,1hr and 2hr post dialysis shown in the picture added below , there appears to be loss of or dilution of protein as in the picture added below. Protein is around 16.5kda
What can be the reason also what can I do to stop the same from happening? Kindly help
does adding Triton to the bacterial culture medium prevents the formation of inclusion bodies and lead to an increase in protein solubility in the cytoplasm?
Methods: interviews w teachers, focus groups, observation) Leaning towards social constructivist, but was told I should probably broaden my search. Help!
I was going to use Hergenhahn's: An Introduction the History of Psychology, but I am really unhappy with the book's treatment of the intelligence testing era of the late 1800s and early 1900s, which was responsible for all manner of social injustice. The term "retardation" is repeatedly used without any explanation of the historical context of the term and without fully appreciating the discrimination and oppression engendered by it (largely as a result of the intelligence testing craze in the West). I'm afraid of requiring my students to read this, especially because I work at an institution with a large population of students with disabilities.
Does anyone have a suggestion for a text that treats social stratification and its consequences on disenfranchised groups with more empathy and greater care?
I am the retird professor in psychology of Leiden University Dolph Kohnstamm.
Esearchgate is sending my Routledgebook Piaget: Chilfren and the class inclusion problem toANYONE IN THE WORLD WHO ASKS for it. Now,in return for that,I askredearchgate to send me all the chapters of the Routledgebook on Phenomenology.
Sincerely Yours. Prof. Em. Dolph Kohnstamm, Amsterdam
A little dilemma here. My team and I are conducting a systematic review on the psychosocial needs of newcomers for integration into Canada. We will include qualitative work that would generate insights into the needs and barriers that various newcomers experience - using an intersectional lens. Where we are debating is the inclusion of dissertations.
I have come across some incredibly relevant dissertations and I feel that because of the rigorous process of checks and reviews they go through, they are as reliable as peer-reviewed articles (if not more). But -
1. What are the arguments against not including theses/dissertations at all, if any?
2. Do we include both Master's theses and doctoral dissertations or only the latter?
Your ideas and insights on this would be extremely valuable to our process. Let me know if you require more information on this.
I recently got this message with a rejection to upload a preprint to ArXiv which is currently published in a peer-reviewed Q3 journal:
"While we acknowledge that this article has been published, our moderators determined it is not of plausible interest for inclusion within arXiv. As a result, this submission has been declined."
Do moderators in ArXiv act as professional and authorized reviewers for whatever subject the paper is submitted to their website?
Spontaneous fracture of tempered glass is usually caused by expansion of phase transformation of NiS inclusion. I am curious about the size of fracture mirror of this kind of fracture. In practice, it is difficult to locate the fracture mirror around the NiS inclusion. I don’t know whether the fracture mirror is basically missing from this kind of fracture or it is just difficult to observe.
I am attempting to analyse secondary data of banks like their financial inclusion index, growth etc. I am looking for sources which explain what are the different techniques of analysis, how to apply them
I am working on this topic of impact of inclusion and exclusion of stocks from an index and its impact. What areas do i have to consider apart from the impact on prices and volumes. If I want to study the impact on Mutual funds how do I go about it?
i want to create knowledge on role of health promotion in inclusion education . do anyone have more idea in it?
Esta pregunta la realizo para saber si actualmente la sociedad incluye a las personas con diversidad funcional y a raíz de ahí, poder investigar sobre ello.
Me gustaría investigar sobre la existencia de garantías para verificar que la inclusión realizada con determinados colectivos y grupos en situación o riesgo de exclusión sea real. Y en el caso de que estos funcionen, que estas garantías sean útiles.
While performing sonication i am facing the issue of frothing which i think results in the aggregation of desired protein and leads to the formation of inclusion bodies
There is an influence of the volume of private credit to the private sector on the Financial Inclusion Index, What is the economic explanation for this
What are the processes that can be implemented to ensure the validity of the application of the inclusion and exclusion criteria in a literature review?
Mach's Principle is gernerally ignored in modern physics. Many have tried to include it. But data indicates it is needed. So, in addition to uniting GR and QM, Mach's principle should be necessary.
I want to investigate the debonding behavior of the matrix-particle interface in a particulate composite with spherical particles in a two-dimensional matrix, using the Abaqus Dynamic Explicit solver.
I used General Contact (Explicit), with cohesive behavior and Johnson -cook damage formulation. However, stress concentration is occurred at matrix, and the stress is not transferred to the particles. Also, interface perpendicular to force direction is deboned very early. I think my interface definition is not correct, any help would be highly appreciated.
I am experiencing some troubles in my fish gonad samples included in paraffin, and I would like to ask you some advices.
The protocol I am using has been already used for years (sea urchin gonads, mullet gonads, oysters...) and it has always worked well. The only things I changed are the type of paraffin (now I am using Leica Paraplast 56°C, before a paraffin with higher melting point) and I started to make practice with the histomolds (plastic cassettes + metal molds).
Recently, I included some small pieces of mullet gonads (4x3x2 mm, more or less) and I tried to cut them at 5 microns. I succeded in creating the ribbon, but when the blade met the tissue, it crumbled. Tissue seems very hard in some samples (like crystallized) but it seems normal in others, and the slices came out with a hole in correspondence with the tissue.
I don't know if I failed the infiltration (oven set at 59°C), or if the dehydration - clearing steps are wrong. The strange thing is that the protocol has always worked perfectly, with small or bigger tissues.
In your opinion, what am I doing wrong? I attach the whole protocol below.
fixation in 10% formalin
wash in water
70% alcohol (1 change/day, x2) before processing
1h 70% alcohol
1h 80% alcohol
1h 96% alcohol
1h 100% alcohol
1h 100% alcohol
1h 1:1 100% alcohol : Bioclear (xylene substitute, natural terpenes)
30 min Bioclear
30 min Bioclear (in oven at 59°C)
Paraffin (Paraplast) overnight (in oven at 59°C)
3h new Paraffin (in oven at 59°C)
You may consider that we do not have an automatic tissue processor so we have the need to block the protocol in the evening. Even for the inclusion step, we don't have neither paraffin dispenser nor embedding station. I store my melted paraffin in the oven, I pour it in the metal mold positioned on a hot plate (paraffin remains very hot and melted), I put the piece of tissue in the mold, I transfer the mold on a reusable ice pack to create a thin solidification of paraffin, then I put a plastic cassette on top, I fill it with more melted paraffin and I put all on the ice pack to cool fast.
Thanks in advance.
I am a PhD student studying inclusion and teaching foreign languages to learners with special educational needs. A few months ago I started my research of inclusion in rural schools in Russia. I see it as a qualitative research with text analysis of several interviews of rural teachers. I thought that problems of rural schools are similar to a large extent in different countries, and it would be interesting to compare the results and write up an article together.
So if there is anyone who would like to participate in this research in other countries and work together at the article, please let me know.
When conducting a systematic review and mete-analysis of observational studies we included two studies which are two secondary analysis of the same original RCT, but each are reporting different numbers according to the same outcomes. I think that inclusion of both enriches our analysis as each study reports different sample size and numbers.
I ask if this inclusion is right or we will get comments on this point by the reviewers?
From experiment, I have found that the curcumin pyrazole-hydroxypropyl beta cyclodextrin inclusion complex showed more phenolic content compared to either curcumin or curcumin pyrazole prepared. Can anyone help me with possible explanation?
I would like to add that the amount of the complex was taken more for the assay based on the loading efficiency of curcumin pyrazole (as loading was not 100%, hence I took more amount of complex to maintain the equivalent amount of curcumin for the assay)
Dear Researchgate Community,
I need your suggestions for methods that can be adopted for gender inclusion assessment at different levels. The focus of this topic shall remain on social life and community development.
We are doing a systematic review and the majority of the studies meeting our inclusion criteria are case series. We are unable to find any risk of bias assessment tool specifically for case series. Can you suggest any risk of bias tool for case series?
Discrimination is a topic in many ways. Students at different universities report discrimination in the context of the pandemic. They experience discrimination due to their disability, their social background, their religion, their nationality a.s.o. Do you know research projects that systematically investigate discrimination at universities?
When evaluating a policy, why is it that social credibility is not given priority than the political acceptability?
I‘m working on a bacterial protein, predicted to be 55kDa and localized to the inner membrane. I’m able to get the protein expressed to reasonably high levels in inclusion bodies but never folded correctly in the membrane, and haven’t been able to optimize this. I went for a denaturing purification and tried both 8M urea and 6M GuHCl to solubilize the inclusion body fraction. I can detect the protein in most fractions as a band that only barely enters the separating gel, at an apparent size of > 200 kDa (See picture). This band is reactive in a Western, but it seems to be insoluble even after boiling in SDS sample buffer (2% SDS & 10mM DTT). The protein has 7 Cys but I kept 10 mM DTT in all denaturing buffers to fully reduce it. Still, I see this band, sometimes very smeared - I load urea directly on the gel (4M after dilution in sample buffer) but precipitate the GdnHCl samples and bring back in SDS sample buffer.
I don’t have much experience with denaturing purification but it seems odd that the protein can’t be denatured/resolved in SDS. How can I analyze the fractions properly? Would adding 8M urea to my SDS loading buffer likely resolve a proper monomer band for this protein? Any advice here would be helpful.
As clearly stated in the title, I am running an international, retrospective, multicentre, observational study of paediatric patients with vascular complications after liver transplantation. To be eligible for inclusion, they should be transplanted between 1-1-2001 and 1-1-2020, and and diagnosed/treated between 1-1-2001 and 1-1-2021.
Would it be prevalence as we are studying proportion of patients who have developed complications at particular time periods? OR incidence given the fact that these complications are an undesirable events?
Here is an example of our study: doi: 10.1002/lt.26412
Thanks and regards,
How can we select the drug which is useful for inclusion complex formation. So that it can increase the threptic efficiency of the drug.
in materials science this word has a negative meaning, "the inclusion of sulfur in steel". but how to describe a composite, an alloy, where is the "inclusion" of intermetallic in the matrix? Where "inclusions" are positive meaning.
My insert is cloned into pcDNA3.1. The full insert sequence shows the surrounding vector sequences including plasmid promoter and terminator are clean and tidy. The 3’UTR of the gene of interest is also present in the insert.
My question is that will COS cells recognise the transcription termination signal in the insert and will this effect the expression of my gene of interest using the pcDNA3.1 vector. Will the inclusion of 3’UTR have a possible effect on the efficiency of termination??
Hope to hear from you soon.
Thank you :)
Hi, I am running a logit regression in Stata. I do not see standard errors and p values in some regressors (for a few models) and in all regressors ( in some other regression models).
Pseudo R square is 1 in the models where SE and p value corresponding to no regressor is reported by the Stata.
I understand that this is happening due to either the problem of separation (quasi or full) or reduced degrees of freedom due to inclusion of higher number of regressors (sort of k>n).
Details for dataset are as follow:
--> Model includes a regressor in the level form as well as the square term (quadratic function).
--> In addition, the model includes 9 control variables plus industry and year controls.
-> Industry includes 34 unique industries in form of 2 digit industry codes.
--> Year control includes 11 unique years. Cross-sectional data is pooled across those 11 years.
If I remove industry effect controls the results become inconsistent in the models. Somewhere these give opposite signs and in some models these become insignificant.
I would be grateful your kind suggestions.
If you work in primary education or with primary aged pupils either who have or do not have SEN.
Then would you consider filling out my questionnaire as part of my dissertation into professionals perspectives and definitions of inclusion.
If you could follow the link below that would be greatly appreciated. Thank you!!
I'm doing a scoping review in the field of robotics and neurosurgery but with a specific topic. I chose scoping review to assess the gap of knowledge on the topic being investigated.
After doing the search (PubMed, EMBASE, and Scopus) and assessing according to inclusion and exclusion criteria I finished with only 3 articles. Can I continue writing my paper or three articles are insufficient to make a scoping review?
I want to observe the soluble fraction of my E.coli cell lysate after sonication. I also want to observe the inclusion body and membrane fraction along with getting idea about any unbroken cells?
What centrifuge speed (in RCF or g) should I use to fractionate them (Time?)
I want to test the impact that regulation has on the continued struggle that Nigeria is facing with financial exclusion. I want to correlate regulations with corruption and heightened government negligence. What research technique is best to adopt. I intend to use data on the economy, financial inclusion and corruption indexes for the last 10years and the regulatory dynamism of the financial sector over the same period
I do not recommended readings on related subject matter.
I am investigating engagement and inclusion with the natural environment with ethnic minorities. The research is designed to culturally competent and sensitive. I plan to use visual research methods and interviews. I have a photography task that i wish my participants to undertake but am aware that not everyone has a smart phone / camera or will be comfortable using such a device in a a public place. My possible solution is to offer other ways to participate ( i.e. diary, art or secondary images) but cannot find any literature on using different methods per participant. The visual data will be used a discussion point in interviews, not to be analysed.
Any thoughts or suggestions welcome.
Do you have any examples of when allyship generated positive outcomes for equality, diversity, and inclusion in an organisation? Are there any cases where allyship caused unintended consequences or even negative results?
Hello, I am a PhD student and I am looking for the topic of financial inclusion and what are the requirements to achieve it. With linking monetary policy variables and indicators of financial inclusion, can you help me?
I understand that quantitative and qualitative research may have certain criteria for reliability and validity checks. However, to think of same with SLR appears a bit new to me at the moment. Could this be the inclusion and exclusion criteria used for selection or something else?
I will appreciate your constructive views, guides, or any useful resources you may offer. Thanks in advance!
I hope 2021 has been a good research year for you.
2022 is almost here.
Is anyone interested in collaborating in any of the broad topics below in 2022?
1. Financial inclusion
2. Sustainable development
3. Bank behaviour and performance
4. Digital finance
5. Circular economy and finance
6. Central bank digital currency
8. Financial reporting
9. Sustainable finance
10. Climate change and finance
11. Forensic Accounting
Hi every one
I have produced my recombinant proteins for my thesis.
I have a problem with purification because this proteins have made inclusion bodies.
I resolved in Urea 8M, It dont purified with Ni Column (with His tag), Amicon Filter.
and when we remove Urea (exchange with PBS), it precipitates.
what should I do?
A reviewer is requesting the inclusion of the country effect in our model. We are using the fixed-effect model and it automatically omitted the country effect.
Any idea, how to explain to a reviewer why country/industry effect cannot be included in a fixed-effect model.
Inclusion should precede diversity in my opinion. Without inclusion, there can be no diversity. Despite this clarity, there is less evidence of the same taking place in practice. I wish to explore further any antecedents or explanations or perspectives on the same.
There is a growing demand and recommendations for corporate boardroom to improve cybersecurity policy and governance. One of the top recommendations was the inclusion of cybersecurity experts at the C-level for boardroom leverage and mitigations implementation. There are contentions on the inclusion of cybersecurity professional as a qualifier for the boardroom.
Because of the inclusion criteria of the search very few studies meet the criteria we were looking for. So, I wonder, can we submit to a journal a systematic review which based the conclusions just on three studies? Is there any guideline about that?
There were different methods adopted to use sample size calculation. I am very concerned about the limited number of patients, who meet the inclusion criteria and exclusion criteria of the study.
Hello fellow researchers!
I am currently working on a systematic review in neurosurgery and I've run into a small issue.
Some of the papers that I am currently screening have sub-groups within them, and some of them meet the inclusion criteria and others do not. Assuming I have groups A, B, C and D (all are distinct), groups A and B meet the inclusion criteria and are included, whereas groups C and D do not and are excluded. All statistical technicalities aside, can I include the paper and extract data from groups A and B ONLY and leave out C and D? If yes, will there be any non-statistical drawbacks? Thank you!
I am launching this topic, which I consider relevant in the context of current challenges, regarding the role and importance of economic, financial, social inclusion and good governance in the global society, with a direct focus on inclusion and diversity in the business environment, it can be a pillar of the recovery, resilience, and progress of the business environment; equity and social inclusion and gender equality (implicitly the role and importance of women in the family and at work); supporting and strengthening inclusion in the way of working remotely in as many trades as possible; the policies approached by companies will have consequences on gender equality in the future. Public-private partnerships based on the principles of the collaborative economy can contribute to stimulating recovery, resilience, and promoting greater equity and can contribute to the well-being of the individual, with a direct impact on societal development.
At the end of 2021, first of all, I wish you a healthy New Year 2021, with personal and professional academic results.
The 2050 European Green Agreement, as well as the Global Green Agreement, emphasized that it should be easier for investors and companies to identify environmentally sustainable investments and ensure that they are credible. This could be done through clear labels for retail investment products and by developing a green bond standard that facilitates environmentally sustainable investment in the most convenient way. For example, in its 2021 work program, the European Commission emphasized "a people-friendly economy" as a priority and emphasized the importance of continuing to make progress on sustainable financing, in particular by proposing a European standard on green bonds. (Impact assessment accompanying the Communication "Enhancing Europe's climate ambition in 2030
Investing in a climate-neutral future for the benefit of our people”(SWD / 2020/176 final). Furthermore, it introduces a set of rules that issuers of green bonds must follow in order to call a bond a "European green bond" or "EuGB", as well as for green bonds issued by the World Bank or other global financial institutions.
I have a protocol to express a enzyme (human 17B-HSD1), but it doesn't list the shaker speed, just the temp (18C for 12-18 hours). I obviously don't want inclusion bodies but I do want to maximize the protein I get. Is 250-300rpm too much?
If you are in a school leadership position in the United Kingdom, or an educational professional involved in teaching in UK schools or colleges , please contribute to my research survey/project (anonymously) on The Development of Inclusive Cultures, in UK Schools and which can be accessed via my Research Project recently added here under my profile.
Alternatively, if you would like to contribute to my research, without completing the survey, please add your considerations below. All contributions will be valuable and gratefully received. Thank you for your time and interest in my research.
For example when your target sample was 400 subjects, among this sample 360 subjects met the inclusion and exclusion criteria for the study, therefore the new sample will be 360 subjects, does it required to calculate the attrition rate?
I have had to withdraw a manuscript I submitted for publication because the reviewer insisted that I include work which I consider tangential to my focus in a paper recently. While I found all their criticisms valuable I considered their insistence for inclusion of particular papers which in my opinion are tangential to the work as unacceptable. It does appear that reviewer's fail to understand an author's perspective and on account of their reviewer status have operated to force their views and orientation on other people's works. They have in many instances completely diverted many an author's view point to their own which practice is only working to narrow perspectives to issues.
It has been a little difficult to wrap my head around the process of this quantitative systematic review procedure. I used PRISMA to create a flow-chart of the 25 studies that met my inclusion criteria. Now as I further analyse the chosen studies, should I use STROBE to delve deeper into the studies and ROBIN-I for my check for publication bias? Or is STROBE not really necessary?
All studies are observational.
Thanks in advance!
In a logistic regression model after including interaction effect R2 increases significantly, but the p value of interaction term is not significant. Should the interaction term be included in the final model ??
I am trying to prepare inclusion complex with HP-beta- CD with the guest molecule. I am facing solubility issue as my guest molecule is very less soluble in ethanol and methanol. It shows good solubility in DMSO. please let me know what preparation method should I try for the inclusion complex preparation? as for freeze drying guest molecule should show solubility in ethanol i guess?
As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria?
My research participants have been chosen based on the inclusion criteria (age 18-35, living in area X). At first, I had distributed my research among my friends and then they spread mine to their social circle. Also, I had posted my research in Twitter to reach the target participants who are out of my social circle and their participation was based on their willingness to volunteer. Can this be regarded as random?
I have an idea for a book on diversity and inclusion, and I am looking for a co-author who does research in that area (preferably in sociology, but anyone who knows the current literature in the area). I recently published a book on the Constitution with a co-author that is a presidential scholar (see https://www.amazon.com/Preamble-Policy-Guidebook-Governance-Civic/dp/1433188236/ref=sr_1_1?dchild=1&keywords=iron+twombly&qid=1630508115&sr=8-1), and I have an idea for a similar book in the area of diversity and inclusion. I am looking for someone who can ground my ideas in the literature. I am willing to share more specifics on the idea with a qualified interested potential co-author.
This project of thesis aims to discuss developments in the field of finance driven by technological developments. The thesis discusses the current challenges facing the adoption of many financial technology solutions on a large scale in MENA countries with the aim of enhancing financial inclusion for all residents of the region. In recent years, MENA governments have paid increasing attention to this technology and the cases of its use as a tool for digital transformation; it has been seen as an engine of economic diversification and has been high on the development agendas of many countries in the region.
Which econometric model fits this topic?
I am planing to write systematic review in rare disease. My inclusion and exclusion criteria meets 35 research articles. However, most of the studies are retrospective and only limited studies have case-control and clinical trials.
Is it appropriate to include these studies together in systematic review (retrospective studies without control)?
Result of inclusion of an independent variable (continuous) when included, among others, in R, says
algorithm did not converge
fitted probabilities numerically 0 or 1 occurred
When done similarly in SPSS gives p values of 0.99 or 1 for all variables and also for the constant.
But when the variable is removed, this gives reasonable-seeming p values for all other variables included (categorical and continuous). The results of p values and estimates are same in R and SPSS in this case.
The relationship of the very independent variable with the dependent variable is: