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Inclusion - Science topic

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Questions related to Inclusion
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how can I overcome inclusion bodies formation during IPTG induction when I want to express a protein with disulfide?
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IB formation could be due to several different reason (eg. protein is toxic, protein is hydrofobic, protein is unfoklded)
if you thibk that the IB formation is due to the fact that disulfides are not correclty formed (which is possible in cytoplasmic expression in E.coli, since the E.coli cytoplasm is reducing) you can try to use the following different approaches to promote the formation of the S-S bonds
1) Use of E.coli strains (as Origami or T7 shuffle) which lack enzimes as Glustaredoxin involved in S-S reduction and promote S-S bond formation in cytoplasm
2) Add to you protien a signal peptide (eg pelB or OmpA leader peptides) for protein expression in the periplasm which iis more oxidating enviroment and purify the protein from it, by performing selective proten extration using osmotick shock.
good luck
manuele
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Discrimination is a topic in many ways. Students at different universities report discrimination in the context of the pandemic. They experience discrimination due to their disability, their social background, their religion, their nationality a.s.o. Do you know research projects that systematically investigate discrimination at universities?
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There are no active projects
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When evaluating a policy, why is it that social credibility is not given priority than the political acceptability?
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policy is related with social and with out society there's no speak or space for the policy
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I‘m working on a bacterial protein, predicted to be 55kDa and localized to the inner membrane. I’m able to get the protein expressed to reasonably high levels in inclusion bodies but never folded correctly in the membrane, and haven’t been able to optimize this. I went for a denaturing purification and tried both 8M urea and 6M GuHCl to solubilize the inclusion body fraction. I can detect the protein in most fractions as a band that only barely enters the separating gel, at an apparent size of > 200 kDa (See picture). This band is reactive in a Western, but it seems to be insoluble even after boiling in SDS sample buffer (2% SDS & 10mM DTT). The protein has 7 Cys but I kept 10 mM DTT in all denaturing buffers to fully reduce it. Still, I see this band, sometimes very smeared - I load urea directly on the gel (4M after dilution in sample buffer) but precipitate the GdnHCl samples and bring back in SDS sample buffer.
I don’t have much experience with denaturing purification but it seems odd that the protein can’t be denatured/resolved in SDS. How can I analyze the fractions properly? Would adding 8M urea to my SDS loading buffer likely resolve a proper monomer band for this protein? Any advice here would be helpful.
Thanks!
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My previous lab worked with a transmembrane protein and were able to purify the protein domains via denaturation using 6M GuHCl. We refolded, dialyzed, and concentrated the protein to visualize the purified and functional protein. Have you tried visualizing the protein after refolding and concentrating?
Also, for the full-length transmembrane protein, I used to heat the samples at about 80degC for 10 minutes rather than boiling the samples. I heated the protein in RIPA lysis buffer which helped.
Good luck,
Meera
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Dear all,
As clearly stated in the title, I am running an international, retrospective, multicentre, observational study of paediatric patients with vascular complications after liver transplantation. To be eligible for inclusion, they should be transplanted between 1-1-2001 and 1-1-2020, and and diagnosed/treated between 1-1-2001 and 1-1-2021.
Would it be prevalence as we are studying proportion of patients who have developed complications at particular time periods? OR incidence given the fact that these complications are an undesirable events?
Here is an example of our study: doi: 10.1002/lt.26412
Thanks and regards,
Bader.
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"Prevalence" is the number of occurrences in the whole population studied. Individual rates could still be subdivided between arterial vs. venous, stenotic vs. thrombotic, whole organ vs. live donor, whatever so longas population size remains adequate for analysis. "Incidence" is the number of NEW events in a population under study. You might check with your institutions' statistician, but I believe incidence is not a retrospective descriptor. Looking back over time for vascular events will give you the PREVALENCE in the (sub)population studied. Finding associations explaining prevalence in your retrospective study (perhaps using analysis of variance) could lead to changes in operative strategy to reduce the incidence of that problem moving forward. For example: suppose the "prevalence" of arterial thrombosis was 5% overall, but 10% with a greater than 4mm diameter mismatch between donor and recipient. Changing surgical technique would hopefully reduce the "incidence" of this complication moving forward. But that's another study...
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How can we select the drug which is useful for inclusion complex formation. So that it can increase the threptic efficiency of the drug.
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Cyclodextrins have both hydrophobic and hydrophilic character and are capable of encapsulating a hydrophobic drug molecule and forming guest-host complexes.
In medicine, cyclodextrins primarily act as a complexing vehicle and consequently serve as powerful drug delivery agents.
cyclodextrins are relatively easy to make. Starch is treated with a combination of common enzymes, notably cyclodextrin glycosyl transferase and α-amylase. Each enzyme combination produces a characteristic ratio of the three cyclodextrins.
I hope this answer will help you little bit ..
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in materials science this word has a negative meaning, "the inclusion of sulfur in steel". but how to describe a composite, an alloy, where is the "inclusion" of intermetallic in the matrix? Where "inclusions" are positive meaning.
Maybe, "particles"??
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Some synonymous terms:
"embedding",
"entrapment"
"enclosure"
"enclave"
" ... in association with..."
"constituent"
"component"
"part"
"unit"
"item"
"ingredient"
"element"
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Hi All,
My insert is cloned into pcDNA3.1. The full insert sequence shows the surrounding vector sequences including plasmid promoter and terminator are clean and tidy. The 3’UTR of the gene of interest is also present in the insert.
My question is that will COS cells recognise the transcription termination signal in the insert and will this effect the expression of my gene of interest using the pcDNA3.1 vector. Will the inclusion of 3’UTR have a possible effect on the efficiency of termination??
Hope to hear from you soon.
Thank you :)
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Usually, 3'UTR can contain regulatory elements (such as miRNA binding sites).I would avoid them if they are not neccessary. However, it should still work.
Double terminators have been shown to increase protein yield in plants and in mammalian cells as far as I remember.
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Hi, I am running a logit regression in Stata. I do not see standard errors and p values in some regressors (for a few models) and in all regressors ( in some other regression models).
Pseudo R square is 1 in the models where SE and p value corresponding to no regressor is reported by the Stata.
I understand that this is happening due to either the problem of separation (quasi or full) or reduced degrees of freedom due to inclusion of higher number of regressors (sort of k>n).
Details for dataset are as follow:
--> Model includes a regressor in the level form as well as the square term (quadratic function).
--> In addition, the model includes 9 control variables plus industry and year controls.
-> Industry includes 34 unique industries in form of 2 digit industry codes.
--> Year control includes 11 unique years. Cross-sectional data is pooled across those 11 years.
If I remove industry effect controls the results become inconsistent in the models. Somewhere these give opposite signs and in some models these become insignificant.
I would be grateful your kind suggestions.
Thanks you!
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Hello Sahil, based on what you just said the problem seems to be too many IVs for your number of observations . I understand that you would like to include industry effects but you don't have enough observations for that big a model. Therefore increased n or decreased number of predictors seems to be your options. Best wishes David Booth
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Hi everybody,
I am experiencing some troubles in my fish gonad samples included in paraffin, and I would like to ask you some advices.
The protocol I am using has been already used for years (sea urchin gonads, mullet gonads, oysters...) and it has always worked well. The only things I changed are the type of paraffin (now I am using Leica Paraplast 56°C, before a paraffin with higher melting point) and I started to make practice with the histomolds (plastic cassettes + metal molds).
Recently, I included some small pieces of mullet gonads (4x3x2 mm, more or less) and I tried to cut them at 5 microns. I succeded in creating the ribbon, but when the blade met the tissue, it crumbled. Tissue seems very hard in some samples (like crystallized) but it seems normal in others, and the slices came out with a hole in correspondence with the tissue.
I don't know if I failed the infiltration (oven set at 59°C), or if the dehydration - clearing steps are wrong. The strange thing is that the protocol has always worked perfectly, with small or bigger tissues.
In your opinion, what am I doing wrong? I attach the whole protocol below.
fixation in 10% formalin
wash in water
70% alcohol (1 change/day, x2) before processing
Processing:
1h 70% alcohol
1h 80% alcohol
1h 96% alcohol
1h 100% alcohol
1h 100% alcohol
1h 1:1 100% alcohol : Bioclear (xylene substitute, natural terpenes)
30 min Bioclear
30 min Bioclear (in oven at 59°C)
Paraffin (Paraplast) overnight (in oven at 59°C)
3h new Paraffin (in oven at 59°C)
inclusion RT
You may consider that we do not have an automatic tissue processor so we have the need to block the protocol in the evening. Even for the inclusion step, we don't have neither paraffin dispenser nor embedding station. I store my melted paraffin in the oven, I pour it in the metal mold positioned on a hot plate (paraffin remains very hot and melted), I put the piece of tissue in the mold, I transfer the mold on a reusable ice pack to create a thin solidification of paraffin, then I put a plastic cassette on top, I fill it with more melted paraffin and I put all on the ice pack to cool fast.
Any suggestion?
Thanks in advance.
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if the whole paraffin is crumbling, that is the ribbon, the knife may be blunt or the paraffin is too soft.
(2) If the paraffin ribbon is cutting well, that is, the ribbon is not tearing nor crumbling then your dehydration, clearing and/or infiltration has fault. If your specimen stained longer than necessary in dehydrating agent or in the infiltrating oven or in the clearing agent especially in xylene, the specimen become hardened and brittle and it will be crumbling while cutting.
Thanks.
Akinloye A. J. PhD.
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If you work in primary education or with primary aged pupils either who have or do not have SEN.
Then would you consider filling out my questionnaire as part of my dissertation into professionals perspectives and definitions of inclusion.
If you could follow the link below that would be greatly appreciated. Thank you!!
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No I don't
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I'm doing a scoping review in the field of robotics and neurosurgery but with a specific topic. I chose scoping review to assess the gap of knowledge on the topic being investigated.
After doing the search (PubMed, EMBASE, and Scopus) and assessing according to inclusion and exclusion criteria I finished with only 3 articles. Can I continue writing my paper or three articles are insufficient to make a scoping review?
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Hi Muhammad, I agree with Suraj's point and it sounds like you are investigating a cool topic. To broaden your search you could try data bases such CINAHL, Web of Science, and Medline. I found it extremely beneficial to consult with some librarians to assist in developing my search syntax (particularly as I found some subtle differences in the syntax as I searched between databases). This might help broaden your search.
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Scopus Updates March 2022 (Inclusion and Exclusion List)
- Books
- Journals
- Conferences
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Scopus updates Mars 2022 inclusions uses to facilitate academic publications while exclusion list support the academic level by reflecting achived researches
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Dear Colleagues,
We are currently conducting a qualitative meta-analysis and looking for qualitative research that is focused on transgender, non-binary, and gender diverse people’s experiences of gender identity development and how their gender functions to support coping with minority stress as well as increasing resilience and flourishing. We are looking specifically at articles that focus on participants in the United States. We are contacting scholars to ask if they have authored or are aware of studies that may meet our inclusion criteria, particularly any new or unpublished studies. Our team will screen the articles to determine if they meet our inclusion criteria. If you know of any studies that may be relevant to our qualitative meta-analysis, please contact or send them to Kelsey Kehoe at kelsey.kehoe001@umb.edu.
Thank you!
Heidi Levitt & Kelsey Kehoe
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Thank you!
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Hi,
I want to observe the soluble fraction of my E.coli cell lysate after sonication. I also want to observe the inclusion body and membrane fraction along with getting idea about any unbroken cells?
What centrifuge speed (in RCF or g) should I use to fractionate them (Time?)
Thanks
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Hi, did you find a protocol for this? I am trying to do this same thing with my bacteria, so any insight would be really helpful.
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Does anyone know how to do a Job Plot for the binding stoichiometry of peptides and cyclodextrin?
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Should I include the mole ratio on Y axis ?
I read some literatures that they plot it
abs* ratio vs ratio . But I did not get the same result as I plot it abs vs ratio .
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I want to test the impact that regulation has on the continued struggle that Nigeria is facing with financial exclusion. I want to correlate regulations with corruption and heightened government negligence. What research technique is best to adopt. I intend to use data on the economy, financial inclusion and corruption indexes for the last 10years and the regulatory dynamism of the financial sector over the same period
I do not recommended readings on related subject matter.
Regards
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There is global consensus on the importance of financial inclusion due to its vital role in bringing integrity and stability into an economy's financial system and its role in sustainably fighting poverty. However, it is more pertinent in the case of some African countries, such as Nigeria as a developing nation, to use financial inclusion as a platform not just for growing the financial sector but more as an engine for driving inclusive economic growth.
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I am investigating engagement and inclusion with the natural environment with ethnic minorities. The research is designed to culturally competent and sensitive. I plan to use visual research methods and interviews. I have a photography task that i wish my participants to undertake but am aware that not everyone has a smart phone / camera or will be comfortable using such a device in a a public place. My possible solution is to offer other ways to participate ( i.e. diary, art or secondary images) but cannot find any literature on using different methods per participant. The visual data will be used a discussion point in interviews, not to be analysed.
Any thoughts or suggestions welcome.
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I think that you talk about visual elicitation interviews. You can use any object or visual product in the interviews. It can be something found, already available or documents that are elicited, provoked. You can use drawings, collages, legos or any other respondent-generated imagery or researcher-produced visuals.
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Do you have any examples of when allyship generated positive outcomes for equality, diversity, and inclusion in an organisation? Are there any cases where allyship caused unintended consequences or even negative results?
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Considero que todos los avances sociales son frutos de una alianza cuyo objetivo es garantizar la igualdad en macroorganizaciones como son las sociedades. A voz de pronto, podemos pensar en la aprobación del matrimonio igualitario en 32 países de todo el mundo (hasta marzo de 2022), aprobación que ha sido consecuencia de una serie de alianzas en cuanto a personas, colectivos, grupos políticos, etc.
Otra realidad puede ser el derecho a la eutanasia y a morir con dignidad, legalizada y garantizada en países como Países Bajos, Alemania, Tasmania, Canadá o España entre otros. Como hecho social, -estemos a favor o en contra-, ha sido fruto de una serie de uniones que han dado respuesta a una necesidad que, desde hace años, pacientes con enfermedades terminales estaban demandando.
Efectos negativos de alianzas, muchos, los más destacables son los conflictos armados como las guerras, ahora más en auge que nunca con la ofensiva de Rusia a Ucrania.
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Hello, I am a PhD student and I am looking for the topic of financial inclusion and what are the requirements to achieve it. With linking monetary policy variables and indicators of financial inclusion, can you help me?
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Hii... Keir.... I'm Mohammad H. Holle. Regarding the question of Financial Inclusion indicators, in the research I did, I used general indicators that are also used by the World Bank and the Indonesian Financial Services Authority. the indicators are: Access, Quality, and Usage. so my brother.
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I understand that quantitative and qualitative research may have certain criteria for reliability and validity checks. However, to think of same with SLR appears a bit new to me at the moment. Could this be the inclusion and exclusion criteria used for selection or something else?
I will appreciate your constructive views, guides, or any useful resources you may offer. Thanks in advance!
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This link maybe useful
"JBI’s critical appraisal tools assist in assessing the trustworthiness, relevance and results of published papers"
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Hi every one
I have produced my recombinant proteins for my thesis.
I have a problem with purification because this proteins have made inclusion bodies.
I resolved in Urea 8M, It dont purified with Ni Column (with His tag), Amicon Filter.
and when we remove Urea (exchange with PBS), it precipitates.
what should I do?
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Hi Seyed,
However, your provided information about your protein is not sufficient, but still, I could suggest a couple of things worth trying. Could you please tell let a bit more about the origin of protein, expression system, protein tag, expression condition, temperature, lysis method and buffer composition? If you have already tried and optimized everything at the expression level (for example low-temperature expression, solubility tag like MBP, GST, SUMO etc, overexpression in presence of ligand, metal ions etc..), you can try a range of detergents to solubilize the protein from inclusion bodies. Urea is a strong denaturating agent and causes the unfolding of the protein. Using a high concentration of chaotropic results in complete disruption of protein structure, so better to replace UREA with some detergent like NP-40, Sarcosin, triton X-100, twin20, DDM etc. You could use a combination of these detergents to solubilize the protein and remove them step by step in further purification steps.
The extreme pH buffer has also been reported as a mild solubilization method. High pH (>12) buffer in combination with 2 M urea has been used successfully for solubilization of IB.
Use low urea concentrations with detergents like N-Lauroylsarcosine and Lauroyl-L-glutamate have also been reported to improve the yield.
If nothing works with your protein and you end up with UREA, then try to remove UREA step by step by following various refolding protocols. For example, Multistep dialysis against a large volume of refolding buffer in the presence of low concentrations of AAs like arginine, proline, glycine etc. Sucrose, sorbitol solutions.
Best of luck,
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A reviewer is requesting the inclusion of the country effect in our model. We are using the fixed-effect model and it automatically omitted the country effect.
Any idea, how to explain to a reviewer why country/industry effect cannot be included in a fixed-effect model.
Thanks
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Stata automatically eliminate it
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Inclusion should precede diversity in my opinion. Without inclusion, there can be no diversity. Despite this clarity, there is less evidence of the same taking place in practice. I wish to explore further any antecedents or explanations or perspectives on the same.
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With International Women’s Day upon us, the need for greater diversity in HE is under the spotlight. And the current requirement for potential STEM students to have studied traditionally related subjects such as maths and physics seems outdated and unnecessary...
It’s no secret that the ongoing lack of diversity in STEM industries causes significant problems. Recent studies have shown that companies with more female employees and with women in leadership positions financially outperform those that are less gender diverse...
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There is a growing demand and recommendations for corporate boardroom to improve cybersecurity policy and governance. One of the top recommendations was the inclusion of cybersecurity experts at the C-level for boardroom leverage and mitigations implementation. There are contentions on the inclusion of cybersecurity professional as a qualifier for the boardroom.
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Dear Wazee Logunleko,
You may want to look at some additional info:
A cybersecurity committee is often tasked with overseeing the development and implementation of an organization's cybersecurity policy, laying out the standards to which employees must adhere to mitigate the company's vulnerability.
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Because of the inclusion criteria of the search very few studies meet the criteria we were looking for. So, I wonder, can we submit to a journal a systematic review which based the conclusions just on three studies? Is there any guideline about that?
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Hi Cibeles Miralles,
Yes it is. Recently we have conducted a empirical study which covers 20,000 + meta-analyses, we emulate the situation that only 3 earliest studies available, 4 earilest, ...., 10 eaerlieest studies available, and compared the results to the full meta-analyses. We found that, in 81% of the situation, 3 studies can achieve the same conclusion to the full meta-analyses.
You may follow our paper, it will be online few weeks later.
Best regards
Chang
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Please be free to brainstorm about this topic. The game industry is booming but there is a gap.
Very few games were designed and evaluated for supporting the learning process of students, especially of those with other linguistic and cultural backgrounds. Gaming among children is a way of communicating inclusion and we should respond to that. We can design games with cultural and inclusive elements and portrait the diversity by digital representations.
If you are interested in co-writing an article to provide a guide to the game industry or brainstorm on this topic, please do so. If you are interested to find a funding together with me for a project like this, email me. Kind regards.
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Results indicated that students who were exposed to the game-based learning within problem-solving method, obtain positive effect on cognitive and affective aspects. Through this research, it provided evidence that the use educational games could support and increase the mathematics learning outcome.
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Dear Researchers.
I hope 2021 has been a good research year for you.
2022 is almost here.
Is anyone interested in collaborating in any of the broad topics below in 2022?
1. Financial inclusion
2. Sustainable development
3. Bank behaviour and performance
4. Digital finance
5. Circular economy and finance
6. Central bank digital currency
7. Cryptocurrency
8. Financial reporting
9. Sustainable finance
10. Climate change and finance
11. Forensic Accounting
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Great, I also interested
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There were different methods adopted to use sample size calculation. I am very concerned about the limited number of patients, who meet the inclusion criteria and exclusion criteria of the study.
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You can also consider qualitative research which is contextual. Multiple case studies or narratives can fit in. You can include participants who are able to articulate
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Hello fellow researchers!
I am currently working on a systematic review in neurosurgery and I've run into a small issue.
Some of the papers that I am currently screening have sub-groups within them, and some of them meet the inclusion criteria and others do not. Assuming I have groups A, B, C and D (all are distinct), groups A and B meet the inclusion criteria and are included, whereas groups C and D do not and are excluded. All statistical technicalities aside, can I include the paper and extract data from groups A and B ONLY and leave out C and D? If yes, will there be any non-statistical drawbacks? Thank you!
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Yes you can
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I am launching this topic, which I consider relevant in the context of current challenges, regarding the role and importance of economic, financial, social inclusion and good governance in the global society, with a direct focus on inclusion and diversity in the business environment, it can be a pillar of the recovery, resilience, and progress of the business environment; equity and social inclusion and gender equality (implicitly the role and importance of women in the family and at work); supporting and strengthening inclusion in the way of working remotely in as many trades as possible; the policies approached by companies will have consequences on gender equality in the future. Public-private partnerships based on the principles of the collaborative economy can contribute to stimulating recovery, resilience, and promoting greater equity and can contribute to the well-being of the individual, with a direct impact on societal development.
At the end of 2021, first of all, I wish you a healthy New Year 2021, with personal and professional academic results.
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Thank you Prof. Yosef Tadesse for your question. Yes, economic, social inclusion and good governance at the societal level are solutions for the global economic future, to which we associate the concept of collaborative economy!
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The 2050 European Green Agreement, as well as the Global Green Agreement, emphasized that it should be easier for investors and companies to identify environmentally sustainable investments and ensure that they are credible. This could be done through clear labels for retail investment products and by developing a green bond standard that facilitates environmentally sustainable investment in the most convenient way. For example, in its 2021 work program, the European Commission emphasized "a people-friendly economy" as a priority and emphasized the importance of continuing to make progress on sustainable financing, in particular by proposing a European standard on green bonds. (Impact assessment accompanying the Communication "Enhancing Europe's climate ambition in 2030
Investing in a climate-neutral future for the benefit of our people”(SWD / 2020/176 final). Furthermore, it introduces a set of rules that issuers of green bonds must follow in order to call a bond a "European green bond" or "EuGB", as well as for green bonds issued by the World Bank or other global financial institutions.
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Thank you all for your answers and I wish you a Merry Christmas and a Happy New Year 2022 full of personal and academic accomplishments with your loved ones!
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Hello everyone,
I have a protocol to express a enzyme (human 17B-HSD1), but it doesn't list the shaker speed, just the temp (18C for 12-18 hours). I obviously don't want inclusion bodies but I do want to maximize the protein I get. Is 250-300rpm too much?
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Most likely, shaker speed won't affect the formation of inclusion bodies. The main effect will be on aeration, but at 18 °C, the growth rate will be slower and oxygen solubility will be higher than at 37 °C, so aeration is probably not a limiting factor. You may consider choosing a lower speed, though, to reduce the mechanical wear and tear of your shaker: 150 rpm should be OK for media such as LB or 2YT. You should also take care of choosing a culture vessel big enough so that the depth of liquid doesn't exceed 4-5 cm to get a good surface:volume ratio for optimal aeration. 1-L Fernbach flasks are a good option for culture volumes up to 700-800 ml.
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If you are in a school leadership position in the United Kingdom, or an educational professional involved in teaching in UK schools or colleges , please contribute to my research survey/project (anonymously) on The Development of Inclusive Cultures, in UK Schools and which can be accessed via my Research Project recently added here under my profile.
Alternatively, if you would like to contribute to my research, without completing the survey, please add your considerations below. All contributions will be valuable and gratefully received. Thank you for your time and interest in my research.
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Leadership develops leadership quality and they believe in work so it administrative power is stimulated by school and it role will continue to be life-long. I crossed that beautiful movement teaches more to me.
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For example when your target sample was 400 subjects, among this sample 360 subjects met the inclusion and exclusion criteria for the study, therefore the new sample will be 360 subjects, does it required to calculate the attrition rate?
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Attrition rates usually refer to participants who drop out of a longitudinal research project. Instead, it sounds like you have simple case of setting inclusion criteria and removing potential participants who did not meet those criteria.
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I have had to withdraw a manuscript I submitted for publication because the reviewer insisted that I include work which I consider tangential to my focus in a paper recently. While I found all their criticisms valuable I considered their insistence for inclusion of particular papers which in my opinion are tangential to the work as unacceptable. It does appear that reviewer's fail to understand an author's perspective and on account of their reviewer status have operated to force their views and orientation on other people's works. They have in many instances completely diverted many an author's view point to their own which practice is only working to narrow perspectives to issues.
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Reviewers must recognize strengths and give constructive feedback to assist the author in resolving problems in the work. Some journals want two sets of remarks from reviewers: one for the author and one for the editor exclusively.
It is perfectly OK to disagree with a reviewer's opinion. You can write the editor a point-by-point rebuttal stating why you disagree with the reviewer's proposal. Make sure your response is supported by evidence. If your point of view is well-founded, the editor will take it into account.
Revise your contribution and explain why you didn't change some of the reviewers' remarks. Describe what you changed or did not alter and why in the cover letter and/or the 'Response to reviewers' document. You should withdraw your manuscript. Object to the editor's choice.
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Please, if yes, tell us the location of the silo, how big it is, and what it stores.
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Isabelle, I know that this type of solution has been used in João Pessoa/PB known as "estacas de compactação": http://ct.ufpb.br/ccec/contents/documentos/tccs/2015.2/pratica-das-fundacoes-na-cidade-de-joao-pessoa.pdf
I suggest also that you contact companies like Tecnogeo and Brasfond. They probably can provide you with information about this type of solution.
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Hello!
It has been a little difficult to wrap my head around the process of this quantitative systematic review procedure. I used PRISMA to create a flow-chart of the 25 studies that met my inclusion criteria. Now as I further analyse the chosen studies, should I use STROBE to delve deeper into the studies and ROBIN-I for my check for publication bias? Or is STROBE not really necessary?
All studies are observational.
Thanks in advance!
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One should follow PRISMA guidelines thoroughly for reporting any systematic review and meta analysis. Here, STROBE reporting guidelines are not required.
ROBINS-I tool is used for assessment of risk of bias of included study.
There is two tools for RoB assessment of observational studies. Robins-I (intervention) and Robins-E (exposure). Select as per the needs.
Then, Use Revman tool for synthesis of extracted data and and GRADE tool for certainty of evidance.
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In a logistic regression model after including interaction effect R2 increases significantly, but the p value of interaction term is not significant. Should the interaction term be included in the final model ??
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It isn't possible for a correctly specified interaction to significantly increase R^2 and have a non-significant interaction term in a general linear model. In a generalised linear model (as Bruce Weaver noted) R^2 isn't uniquely defined so I don't think you can have a significance test of the change in R^2. It is just about possible for the change in deviance for the model to be significant and the Wald test to be non-significant (but unlikely with large n). In these case the change in deviance using the likelihood ratio chi-square (the likelihood ratio test) is more accurate than the Wald test.
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I am trying to prepare inclusion complex with HP-beta- CD with the guest molecule. I am facing solubility issue as my guest molecule is very less soluble in ethanol and methanol. It shows good solubility in DMSO. please let me know what preparation method should I try for the inclusion complex preparation? as for freeze drying guest molecule should show solubility in ethanol i guess?
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Stir the HPCD solution with your guest vigorously (e.g., ultra-turrax) or sonicate for some time, filter the insoluble, and freeze-dry the solution. You can record a solubility isotherm by this method using various HPßCD concentrations. Eventually, you can try more than one HPßCDs (different DS, Degree of Substitution, number of HP groups/macrocycle). It might be that higher DS (e.g., DS~6.3, which is a USA version of HPßCD) works better.
In the case of low DS (3.5-4.5), the amount of mono(2-hydroxy)propyl-ßCDs is noticeable, and they can form even less soluble complexes than the guest alone.
Please remember that in the case of very insoluble guests, the dilution of the complex solution with water may lead to hazy solutions or sometimes precipitation.
DMSO forms complexes with various CDs, and, additionally, it is practically impossible to remove from the complex.
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I have an idea for a book on diversity and inclusion, and I am looking for a co-author who does research in that area (preferably in sociology, but anyone who knows the current literature in the area). I recently published a book on the Constitution with a co-author that is a presidential scholar (see https://www.amazon.com/Preamble-Policy-Guidebook-Governance-Civic/dp/1433188236/ref=sr_1_1?dchild=1&keywords=iron+twombly&qid=1630508115&sr=8-1), and I have an idea for a similar book in the area of diversity and inclusion. I am looking for someone who can ground my ideas in the literature. I am willing to share more specifics on the idea with a qualified interested potential co-author.
Robert Irons
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Thank you sir. I will find the book, and when the time is right, I will take you up on your offer. Your consideration is appreciated.
Robert
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This project of thesis aims to discuss developments in the field of finance driven by technological developments. The thesis discusses the current challenges facing the adoption of many financial technology solutions on a large scale in MENA countries with the aim of enhancing financial inclusion for all residents of the region. In recent years, MENA governments have paid increasing attention to this technology and the cases of its use as a tool for digital transformation; it has been seen as an engine of economic diversification and has been high on the development agendas of many countries in the region.
Which econometric model fits this topic?
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Yes. Exactly right. The financial technologys are used to boost the financial health of the economy of any country.
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Hi,
I am planing to write systematic review in rare disease. My inclusion and exclusion criteria meets 35 research articles. However, most of the studies are retrospective and only limited studies have case-control and clinical trials.
Is it appropriate to include these studies together in systematic review (retrospective studies without control)?
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Hi Nirmal, How are you getting on with this systematic review? Did you include case studies and case control studies?
I can imagine there would be quite a mixture of literature types on your topic.
This may be helpful.
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Result of inclusion of an independent variable (continuous) when included, among others, in R, says
algorithm did not converge
fitted probabilities numerically 0 or 1 occurred
When done similarly in SPSS gives p values of 0.99 or 1 for all variables and also for the constant.
But when the variable is removed, this gives reasonable-seeming p values for all other variables included (categorical and continuous). The results of p values and estimates are same in R and SPSS in this case.
The relationship of the very independent variable with the dependent variable is:
Accuracy=0.898
Sensitivity=0.769
Specificity=1
p-value<0.0001
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It appears that 2 variables contain the same information. I'm attaching a recently accepted paper of ours that had a similar problem and tells how we dealt with it. Best wishes, David Booth
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I am currently completing my MA thesis in SEN and inclusion (autism pathway). My thesis is on the inclusion of autistic students in mainstream secondary schools, and I am conducting the research with SENCo's. I only have two schools who have agreed to participate, is this enough for a multiple-case study design?
Thank you :)
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Hi Erin,
Yes, you have enough participants to complete the study as it stands. Just ensure when reporting the results you don't overgeneralize and you'll be fine.
Best of luck!
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I am trying to use CB(8) and various gels as a part of my PhD project, and wish to do some NMR on it to confirm inclusion within it. But as it is quite insoluble in many solvents or they become a gel in other solvents, I do not know the best way to go about it. Any suggestions would be appreciated!
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What feature the spectra and, or methods can you use to confirm inclusion?
The rate of overall molecular motion determines how much H-H dipolar coupling contributes to the H--1 and/or C-13 NMR line widths.
For solids, you would need to use solid-state NMR methods.
For gels, the answer depends on the viscosity of the sample. H-1 NMR may be possible. The lower the viscosity the more narrow the line widths. If the inclusion complexes are stable above room temperature, increasing the probe temperature could be helpful.
Finally, is there a dynamic equilibrium between inclusion and non-inclusion species? If you are unlucky, the exchange rate could render the relevant lindwidths to be very broad.
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Since the G-20 summit in Seoul in 2010, financial inclusion has been included in the 2030 Agenda of the Sustainable Development Goals. However, Africa still lags far behind other developing regions in the world.
We present a mapping of the level of financial inclusion in Africa, constructed by us from World Bank's global findex database from 2011 to 2017.
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Properly implemented financial integration in Africa will be one of the important factors in the dynamic economic development on this continent. It is important not to make the same mistakes as in the Anglo-Saxon model of the financial system that led to the greatest global financial crisis of 2008. I described this issue, ie the sources of the global financial crisis of 2008, in my publications, which are available on my Research Gate profile. If financial integration in Africa is implemented reasonably and efficiently, i.e. taking into account diligently conducted risk management processes, including credit risk, and taking into account sustainable development, effective use of the huge development potential, including the large resources of various raw materials, Africa may in the coming years to develop effectively and dynamically.
Regards,
Dariusz Prokopowicz
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Hello! I am trying to extract a membrane peripheral protein, which is barely soluble in water, from inclusion bodies of BL21 disrupted cells for a native purification, I was wondering if anyone knows procedures to get it extracted by using non-denaturing and dialysable detergents (DPC, OG, CHAPS, etc) instead fully denaturing it with urea? Or any other ways to extract the protein without having to fully denature it? Thanks in advance for your recommendations!
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You can use salt precipitation
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Currently I am doing a systematic review of the predictors of clinically significant weight gain amongst those who take antipsychotics. This is usually defined as a 7% or more increase in weight. My inclusion criteria will allow for both RCTs and non-randomised studies of interventions. My question relates to distribution of predictor variables. My understanding is that randomisation will allow for even distribution of potential confounders and baseline characteristics of participants. In an observational study, this will likely not be the case. My question relates to whether I should be prioritising RCTs over non-randomised studies of interventions because of this. As regression analysis will just be conducted amongst those who are treated with the drug, will baseline data, some of which might function as a predictor variable e.g. sex, be more evenly distributed if the sample arises through a randomisation procedure or not?
I may be over-complicating this, as if I include for sex in the model - perhaps if accounts for this even if males vs. females are not evenly present within the sample?
Any help much appreciated!
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Dear Ita,
From a technical standpoint, randomization is a requirement for the probabilistic interpretation of the regression model, not the fitting of the model. The purpose of the randomization is to reduce the influence or biases of the researcher in selecting a sample so the sample will fit the probabilistic model, and hopefully, resemble the population. While using nonrandom data does violate an assumption for a pure probabilistic interpretation of the results, it is seldom achieved completely even in published studies. When that is case, the nonrandomness of the data should be included as one of the limitations of the study so consumers of the research can make their own judgments about the fidelity of your results.
Good luck,
Jim
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On a synthetic graphite tube production line, this type of defect, similar to scales or the beginnings of cracks (cf. photos), appears regularly, including on unimpregnated tubes, just out of the oven (on the photos, these are tubes that have been impregnated and then brushed with traces of resin at the bottom of the scale). We noticed that our graphite had an abnormally high Fe content (a supplier problem). Do you think that these defects can come from a cast iron inclusion (the carbon of the graphite + Fe) formed locally during the graphitization process? Can such an inclusion at this scale (0.5-2mm) lead to surface flaking? If not, do you have any ideas on the origin of these defects?
Thank you very much for your answers and time,
A french materials engineering trainee in need of knowledge
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sure it maybe
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Hi, I am a medical graduate working on a systematic review. Thank you for answering my previous question, I have a doubt regarding articles selection whether post hoc analysis and pooled analysis of randomized controlled trials are qualified to be equivalent as randomized controlled trials?
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The safest answer is that this will depend in your pre-specified eligibility criteria for studies to consider, and you have to ensure to avoid considering an included randomized controlled trial more than once as "duplicates."
A study/publication that is explicitly a post hoc or secondary analysis of a prior randomized controlled trial publication, I believe, should only be considered if the prior publication does not fit your eligibility criteria (likely because your outcomes of interest were not considered there, among others) BUT the post hoc/secondary analysis paper does. While this is possible, I think that this rarely happens under exceptional circumstances. However, you have to ensure that the randomized controlled trial qualities are retained in the secondary analysis (sometimes, secondary analyses of RCTs "transform" the resulting work as a study with a non-RCT design). However, if both the prior RCT and the resulting secondary/post-hoc analysis study retain the appropriate qualities and are eligible, then considering both in your systematic review will basically and wrongly "double" the weight of the prior trial in your analyses. This duplication must be avoided.
Regarding pooled analysis of randomized controlled trials (which is basically a smaller meta-analysis, I think), it is likely that the included individual RCTs there fit your eligibility criteria, that is why you considered the pooled analysis to be included in your review. In that case, including the individual RCTs in the pooled analysis paper AND the actual pooled analysis paper will again wrongly "double" the weight of the latter studies in your systematic review. This duplication must be avoided as well.
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I am looking for help on how to formulate a phrase.
I have excluded patients with entirely missing data, but have included cases with only incomplete documentation. How do I include this in my paper and express that therefore statistics and reported results do not align with the total number of included cases?
And where would you advise I put this information? Under the Methods section? Discuss it under limitations?
Thank you, I appreciate your help!
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Hi, I am expressing 25KDa protein in BL21. I have used 1-1.5mM IPTG conc. with varying temperature ( 37C, 20C, 18C) at different time span. However, at 37C, I got maximum expression but it turned out to be inclusion bodies. Protein expression at lower temperature is not satisfactory (16-24hours). So far I have not used 30C and 25C temperature for expression. Can anyone suggest me if I induce cells at this temperature, then how much time will be required for induction. thanks
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Dear Mehwish
if lower temperature was not able to improve protein solubility, i suspect that your protein is unfoled and you have to evalaute the possiblity to change E.coli strain, expression host, protein domain in the basis of the protein sequence properties
eg;
-it contain many cisteines?
- it contain some transmembrane regions?
- It require to bind some cofactors (as metals, sugars or other molecules for its acrivity)
Ciao
Manuele
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We are looking to better understand how people have been using the principles of universal design in either research or evaluation to address equity and inclusion.
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We are using UD in a project that constist in an Inclusive Oechestra. We have developed a Doctoral thesis about UD principles applied to Instrumental Music Making for groups. We have defined speciffic guidelines for each principle and evaluated their impact throug questionaries and focus groups. Results show thatThe musical result is a good indicator of the success of UD application. Also aspects about satisfacion of participants and its continuity in the group are good indicators too.
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As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria?
My research participants have been chosen based on the inclusion criteria (age 18-35, living in area X). At first, I had distributed my research among my friends and then they spread mine to their social circle. Also, I had posted my research in Twitter to reach the target participants who are out of my social circle and their participation was based on their willingness to volunteer. Can this be regarded as random?
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Of course! It can and SHOULD: The "complete" random sampling does not ensure adequate representativeness of the Target Population to be studied -by the laws of probability itself- unless the sample obtains a number of subjects almost similar to said Target Population: Therefore, a prior sociodemographic study of such Population should be carried out in order to know, for example, its distribution by Sex, Age, etc. and any other important variable for the Study in question... in this way, it is advisable to "previously design" the "strata" and "subgroups" -with an "n" to be taken, representative in each of them of the "N" in the Population and, THEN YES, perform in each "stratum", "subgroup", etc. random sampling; Therefore, continuing with the example, an exclusion criterion would be to take more subjects of a Sex or Age, in the sample, than the Population itself has, since said sample WOULD NOT BE REPRESENTATIVE (this is what is known in statistical jargon as "sampling by strata and clusters"). Thanks.
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If employers in global are stating that they are providing equal opportunity, then whats the point in asking personal details such as gender, ethnicity etc
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The point is to ensure they really do it, that they do not only talk about it when in practice nothing happens!
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Reviewing the literature shows that the inclusion complex, obtained by co-precipitation method between a pigment (curcumin, bixin…) and cyclodextrin, is usually washed with solvent (ethanol/acetone) and water to remove the uncomplexed pigment and CD, respectively, before frozen and lyophilized the powder.
So far as I know, the solvent is used to repeated extraction of the included pigment in order to quantify the encapsulation efficiency. In the other hand, the obtained complex is totally soluble in water.
So, how is possible washing the precipitate without the completed dissolution of the inclusion complex?
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No
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Reviewing the literature shows that the inclusion complex, obtained by co-precipitation method between a pigment (curcumin, bixin…) and cyclodextrin, is usually washed with solvent (ethanol/acetone) and water to remove the uncomplexed pigment and CD, respectively, before frozen and lyophilized the powder.
So far as I know, the solvent is used to repeated extraction of the included pigment in order to quantify the encapsulation efficiency. In the other hand, the obtained complex is totally soluble in water.
So, how is possible washing the precipitate without the completed dissolution of the inclusion complex?
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You could lyophilise the CP product.
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I'm looking for studies or conceptual papers on the (potential) uses of AI for enhancing diversity and inclusion within organization. The biases concerning the use of (big) data are known and written about, but how can data support d&i efforts and research?
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Intresting topic. I haven't idea. Best of luck Berger for your research.
Regards@Ali
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Can someone help me with knowing about the prospect of research on financial inclusion?
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Good question
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During 2017, the Journal Citation Reports (JCR) database tracked all impact factors for 12,298 journals. Impact Factors are useful, but they should not be the only consideration when judging quality. Not all journals are tracked in the JCR database and, as a result, do not have impact factors. New journals must wait until they have a record of citations before even being considered for inclusion. The question " Is there any other organization tracked impact factors for journals besides the Journal Citation Reports (JCR)?
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yes
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Hello everyone,
I am using Orbitrap Q-Exactive plus for metabolomics analysis. I would like to ask about the differences between dd MS/dd-MS2 vs targeted SIM/dd-MS2 mode. When I ran the full MS/dd-MS2 (without a inclusion list) I can get the MS/MS fragmentation however, when I tried to run it with targeted SIM/dd-MS2 mode (with a list of 400 precursor ions), I couldn't get any peaks from the analysis.
So I wonder if the targeted SIM/dd-MS2 mode has a limitation in terms of number of compounds included in the inclusion list? Additionally, whether specifying the retention time for each compound will be a better idea in this case?
Beside, will full MS/dd-MS2 with inclusion list would be another option for targeted large number of compounds? If so, is there any limitation for the number of analytes inputted?
Thank you for your help!
Happy new year to everyone!
Best wishes.
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Agreed. RT is absolutely necessary information to set in the inclusion list in tSIM-ddMS2. tSIM-ddMS2 will normally give better sensitivity compared to FMS-ddMS2, but only if the compounds are not co-eluting. Thus, the compounds in the inclusion list for tSIM-ddMS2 should have different RT ranges. I usually only use tSIM-ddMS2 for a limited number of compounds, up to 10 compounds, depending on their separation. But as Luca says, the loop count number determines how many compounds it will perform MS2 scans of, in the same RT range.
In Full MS the entire mass range is measured and seen in the MS1 scan (e.g. m/z 100-1000), whereas in the tSIM scan, only the targeted compound mass +/- isolation window is measured. In the next tSIM scan it will measure the next mass, according to the inclusion list RT. Thus if two compounds are co-eluting - or in your case 400 compounds are "coleuting" (no RT set), it will switch between perfming MS1 scans of one compound and then the other compound, and also doing MS2 scans when the thresholds and triggers are met. With 400 compounds you will get only one MS1 scan of a few of the compounds and no MS1 scans of the others. And you need at least 10 scans of the compound in order to form a good peak.
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Or some formative data to share? Interested in your inclusion of parents in your study...we are looking at the treatment of mental illness in childhood and the impact of parents on this subject.
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it is going to differ according to the various models of delivery globally and resource availability. Has anyone explored the use of Open Dialogue?
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How can the inclusion of agriculture in carbon markets provide significant benefits for farmers?
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The idea is that soil health and agricultural greenhouse gas emissions inventories, would allow for a market to be established so that payments could be made to farmers to offset their costs as they make efforts to improve ecosystem services such as carbon sequestering or to make emission reductions.
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Hi all,
I have been struggling a great deal with this matter and as such I hope you could provide me with answer. I have added a dummy variable to my ARDL model to improve its stability (Eviews). However, if I estimate the CUSUM and CUSUMsq tests afterwards, they start from the point in time where the dummy variable turns 1. However, I am interested in reviewing the stability of the entire time period in scope of my analysis. Does anybody know how to enlarge the scope of the CUSUM and CUSUMsq tests? Curious to hear your thoughts. Many thanks!
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Thank you very much for your answer. I was not aware of this restriction. Would it then be best to adjust the sample of the ARDL model up to the dummy and to run the model without the dummy to assess the stability of its parameters? Many thanks for your suggestion, it is greatly appreciated.
Kind regards,
Owen
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Knowing that DDGS is a byproduct from ethanol plant and is increasingly becoming an alternative diet to livestock due to the increasing demand of Corn and soybean for human consumption. The protein content of DDGS is low as such their is a need to increase the solubility of protein to help increase the inclusion rate of DDGS in Livestock diet pigs in particular. So am looking for microbial strains capable of producing protease that can hydrolyze the protein content of DDGS.
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Emmanuel
0k. Take present that rumen undegraded protein is also important, of course if it can be digested in the lower gut. Best regards
Redimio
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I have a categorical variable with N factor levels (e.g. gender has two levels) in classification problem. I have converted it into dummy variables (male and female).
I have to use neural network (nnet) to classify. I have two options -
  1. Include any N-1 dummy variables in the input data (e.g. include either male or female). In statistical models, we use N-1 dummy variables.
  2. Include all N dummy variables (e.g. include both male and female)
Can someone please highlight the pros and cons of both options in predictive power and interpretability
"Taken from data science stack exchange"
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What are the possibilities and implications in this regard
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It will help farmers only when the government make use of carbon trading and make provision of high incentives for promotion of carbon farming viz. agroforestry, horticulture, organic farming and dairy etc. and ensure premium prices of carbon farming products in sustainable manner.
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I tried to express my protein in BL21(DE3) bacteria. The desired protein MW is 65.5 KDa and I know my protein will be in an insoluble form (inclusion body). I separate soluble and insoluble fractions after sonication and I see an expressed band around 45 KDa. (i wanted to analyze soluble and insoluble fraction beside whole-cell and compare 2 different temperature for the protein expression)
as I see an expression band in whole-cell analysis, I think the chance of proteolytic cleavage is low! I'm also sure about the gene sequence.
what can be the reason?!
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Dear Paratsou
From the gel it seems that you have a protein degradation issue (because you have a >45KDa in the insoluble and 14 in the soluble which together may correspond yo your full length protein.
Which was you induction temperature? 37°C
In this case you can certainly go down to 25°C or 20°C to see if in this way you may reduce the degradation. (proteases are less active at low temperature)
Are you expressing the protein with some tag? and the fragmentation may be localized between protein and ta (in the linker?) or it seems to be localized inside the protein sequence?
Because in the first case you can try to change the linker sequence, while in the last case you have to identify the digestion site (eg performing N-terminal sequence analysis by EDMAN approach) and if possibile try to remove the digestion site performing single point mutations.
One other approach could be, change the E.coli strain or expression hosts.
For example in the past, for the catalitic domain of TcdA clostidium that i was never able to express in E.coli, because we obtain in insoluble and fragmented form, we moved in Brevibacillus and we were able to express it in folded and active form.
but you have to try many things in E.coli before move on one other organism.
good luck
Manuele
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It's matter of big discussion that traditional medicine may play key role in management of Covid-19. Recently, in India, Ayurveda and Yoga has been included in National management protocol for asymptomatic n mild cases of Covid-19.
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Holistic management is a key concept in management of any ailment. Even WHO acknowledges the varied spectrum and dimensions of health. Aurvedic medicines have been accepted even by the Westerners and stood the test of times. In addition allopathy has borrowed much from traditional medicines. Yoga is also a great supplement to other therapies as it focusses on physical fitness as well as breathing exercises which further improve our respiratory system. Moreover allopathy has not provided yet any concrete evidence of management and are recommending only hit & trial therapies or symptomatic/supportive management. Therefore it's a great initiative to include such alternative system of therapies for the management so that we can enrich our arsenal for the fight against this pandemic.
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Hello
Evidence suggest that technical knowledge and accessibility both for patient and therapist are essential to the effectiveness of tele-psychiatric treatment. The questions as I see it arises when discussing patient characteristics.
Do anyone have experience with what kind of patient characteristics modulates positive effects of web-based or tele-psychiatric treatment, particularly videoconferencing, for
patients with psychiatric diseases?
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In the Covid 19 pandemic process, tele-psychiatric treatment can be preferred, especially for those with virus transmission anxiety, risk groups, those who do not want to leave the house and do not want to go to the hospital.
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I am completing a Bayesian Linear Regression in JASP in which I am trying to see whether two key variables (IVs) predict mean accuracy on a task (DV).
When I complete the analysis, for Variable 1 there is a BFinclusion value of 20.802, and for Variable 2 there is a BFinclusion value of 1.271. Given that BFinclusion values quantify the change from prior inclusion odds to posterior inclusion odds and can be interpreted as the evidence in the data for including a predictor in the model, can I directly compare the BFinclusion values for each variable?
For instance, can I say that Variable 1 is approximately 16 times more likely to be included in a model to predict accuracy than Variable 2? (Because 20.802 divided by 1.271 is 16.367 and therefore the inclusion odds for Variable one are approximately 16 times higher).
Thank you in advance for any responses, I really appreciate your time!
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If you have performed the analysis separately it may be an indirect inference that can be reported, although with the evidence the second predictor does not have any significant frequency effect, in case it is significant and you have performed the regression including both predictors you can refer a possible mediating effect of the first predictor.
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Do you know papers that discuss this subject?
We are looking for objective methods to decide about inclusion/exclusion of data sets.
We are doing a study that looks at the detection rate of an effect (N400) on a single subject basis.
We have some very noisy data sets and are wondering where to draw the line for inclusion.
What criteria/methods do you suggest?
- Sufficient number of trials left per condition after preprocessing
- Sufficient SNR
- ...?
We are looking forward to your replies!
Thank you.
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Dear Anna
It actually strongly depends on what you want to do with your data.
If you just doing a simple amplitude/latency assessment you don't need that high signal quality.
If you want to perform source analysis/time-frequency you need much cleaner data (high SNR). Note that the SNR is defined as a baseline to signal of interest ratio (not the overall quality)
Finally in the case of single single-trial analysis you definitely need clean data
On the other hand, what problem do you have with your data?
Is it high noise? - maybe it can be filtered out
is it an artifact (EKG, blinks)? - that can be easily reduced
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Hi,
I need advice or examples of how to write a paper about a big reimplementation (change of language, inclusion of patterns, possibility of running modules in parallel, etc.) of an open source library. I don’t know if there is a defined benchmark to describe improvements or a defined way.
Regards
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follow
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Hello all,
I am specifically seeking scholarship on the history of students with disabilities, as it pertains to challenges of the past and current obstacles, to higher education inclusion, programming, and retention. Creating a timeline for challenges for this student group.
My thanks!
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Hello Francesca,
Thank you so much!
Grazie!
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Greetings from my side.
I am working on a panel of Asian countries. However, the biggest hurdle I am facing is missing values/data for financial inclusion. In many papers of financial inclusion, authors have mentioned that they applied factor analysis techniques to create an index for financial inclusion by using several proxies.
I am also trying to use the same proxies by doing "Principle Component Analysis" to create financial inclusion index. Due to missing values, the index does not show constant values throughout the years (some years show blank lines), because some countries do not have data at all for some dimensions. Can I assign the '0' to those missing variables?
Kindly advise me on how to deal with this situation or use any particular technique where I do not have a complete set of data. I shall be very grateful to you.​
Best Regards.
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