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Iberian Peninsula - Science topic

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Dear colleagues,
just got some cells from OFK-6 hTERT2 cell line that were sent by a colleague. However when thawing them they were not viable. We used the Keratinocyte serum free media from Gibco (https://www.sigmaaldrich.com/PT/en/product/sigma/131500a?gclid=CjwKCAjwo8-SBhAlEiwAopc9W1gUBbw-9sMB10gLpDM49sN1gYhVc-7uQepsBp71TFywTR4Sjvvj-xoCzaMQAvD_BwE) and followed all the cautions with other cell lines. What could have gone wrong? Meanwhile, is there anyone, ideally in Spain, that could provide us some aliquoted of those cells? Of course we would arranje for shipment to Portugal. Many thanks!
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Hi, Joana,
Gibco provides the supplements for keratinocytes with the media as well that include EGF human recombinant and Bovine Pituitary extract. Did you add these in your media? And you would also need a antifungal and antibiotic to avoid contamination. Usually, with all this taken into account, they grow pretty well. All the best!
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In the last week, some popcorn crops in Catalonia (NE Iberian peninsula) are being invaded by a Photinus firefly. It is an american species, but there are about 300 species and can't find keys for all. Any help, reference or specialist will be wellcommed.
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One problem is the North American Photinus are extremely difficult to rear from egg to adult. It takes a year so requires quite a bit of dedication. However using Michael's method of pairing a male and a female would be a good approach if you just want to see what the first or second instar looks like. They often die after the first or second instar . Rearing Photinus has been a bit of a Holy Grail in the Firefly World. Some of the other genera are not nearly as difficult to rear. Photinus from egg through the larval instars to adult are difficult! But there is someone out there who will figure it out and hopefully tell the rest of us.
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I´m working on a project proposal that will develop a historical event-based model and a historical SDI (snapshot model). I´m wondering which metadata standard suits better for both. The study case is in the Iberian Peninsula.
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In my opinion, you should use ISO 19139 that derived from ISO 19115. The implementation of INSPIRE dataset and service metadata was based on ISO/TS 19139:2007: https://inspire.ec.europa.eu/id/document/tg/metadata-iso19139https://inspire.ec.europa.eu/id/document/tg/metadata-iso19139
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Hello! I'm looking for raster files to create a map of the Iberian Peninsula and also the Minho River, in Portugal. Can you recommend me any websites where I can found those?
The raster files I found only are good for a larger perspective, because when I zoom in on the specific region (Minho River) it looses a LOT of quality.
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Thank you all! I'm still having trouble, since I do not find a single map that does not pixelate when I zoom in my study area. All rasters (even the ones that I could find in QGIS) either pixelated and with very low quality or doesn't allow me to zoom in that much (says map is unavailable).
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I am reviewing the origin of the exotic species in the Iberian Peninsula of Rattus and Mus and found no information about the first entry of the species of Rattus in Spain and Portugal.
Any help will be most useful.
Thank you, Gloria
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Rattus rattus: the introduction of the black rat into Britain | Antiquity | Cambridge Core
Hello Gloria,
Fun to look into this for you, for Rattus rattus in the UK we're talking circa 10-11th centuries about the same time as the crusades. Archaeological digs of Roman sites suggest a range between the most likely earlier in mainland Europe.
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I am currently doing an analysis of climatic effects on the reproduction of the European shag and I need to get the monthly SST averages in the northwest and north of the Iberian Peninsula. Does anyone know where this data could be downloaded? I'd be glad for any help!
Thanks a lot
David Álvarez
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Thanks, Biplab
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I'm looking for Mousterian blade production on Iberian Peninsula. Probably at the Abric Romani ? But don't found any published work about it. In El Castillo and Queva Morin there are some bladelets production.
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Dear Leonardo:
if you mean real blades related with blade cores are difficult to be found. In Madrid at El Cañaveral, Parceka 32, there are some examples, and also in some levels of El Esquilleu, MOorin, Castillo, etc. but the sizes are small. If you just mean elongated flakes there are many in all the levallois levels. Hope it helps you
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We are working on the waste of a workshop dedicated to the production of this type of manufactures located in the Ebro Valley, near Zaragoza, and dating back to the 11th century.
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Dear Hasitha,
Thank you very much for the text you have recommended.
Best regards