Science topic

Histology - Science topic

The study of the microscopic anatomy of cells and tissues of plants and animals, including tissue fixing, fixation and staining.
Questions related to Histology
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Formalin is commonly used preservative for routine histology.
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Formalin is the most forgiving of all fixatives when conditions are not ideal, and there is no tissue that it will harm significantly. Thank you!
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I used an IHC staining for Pan-cytokeratin to test for metastasis from breast to brain. I am not sure if the only cells found in brain that are stained brown-ish are the tumor cells from breast cancer that metastasized or could be any other type of cell already present in the brain?
Thanks!
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Sorry for the late response. I would like to correlate with H&E. Can you please share that? Thanks
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Dear colleagues,
I was wondering whether there are any ways or any softwares that I can use to analysis the muscle cross sectional area in my H&E histology images?
I tried to use ImageJ thresholding, unfortunately it does not work efficiently for me. Thus, I was wondering whether there are any currently established methods.
Thank you very much in advance.
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I also want to calculate muscle fibers CSA in H&E staining using imageJ. Thresholding cannot be used but manually it can be done and it will be time taking process. I want to know about how many and how the fibers are randomly selected in a sample field, how many sample fields will be needed and what should be the magnification?
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Hi everybody,
I am experiencing some troubles in my fish gonad samples included in paraffin, and I would like to ask you some advices.
The protocol I am using has been already used for years (sea urchin gonads, mullet gonads, oysters...) and it has always worked well. The only things I changed are the type of paraffin (now I am using Leica Paraplast 56°C, before a paraffin with higher melting point) and I started to make practice with the histomolds (plastic cassettes + metal molds).
Recently, I included some small pieces of mullet gonads (4x3x2 mm, more or less) and I tried to cut them at 5 microns. I succeded in creating the ribbon, but when the blade met the tissue, it crumbled. Tissue seems very hard in some samples (like crystallized) but it seems normal in others, and the slices came out with a hole in correspondence with the tissue.
I don't know if I failed the infiltration (oven set at 59°C), or if the dehydration - clearing steps are wrong. The strange thing is that the protocol has always worked perfectly, with small or bigger tissues.
In your opinion, what am I doing wrong? I attach the whole protocol below.
fixation in 10% formalin
wash in water
70% alcohol (1 change/day, x2) before processing
Processing:
1h 70% alcohol
1h 80% alcohol
1h 96% alcohol
1h 100% alcohol
1h 100% alcohol
1h 1:1 100% alcohol : Bioclear (xylene substitute, natural terpenes)
30 min Bioclear
30 min Bioclear (in oven at 59°C)
Paraffin (Paraplast) overnight (in oven at 59°C)
3h new Paraffin (in oven at 59°C)
inclusion RT
You may consider that we do not have an automatic tissue processor so we have the need to block the protocol in the evening. Even for the inclusion step, we don't have neither paraffin dispenser nor embedding station. I store my melted paraffin in the oven, I pour it in the metal mold positioned on a hot plate (paraffin remains very hot and melted), I put the piece of tissue in the mold, I transfer the mold on a reusable ice pack to create a thin solidification of paraffin, then I put a plastic cassette on top, I fill it with more melted paraffin and I put all on the ice pack to cool fast.
Any suggestion?
Thanks in advance.
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if the whole paraffin is crumbling, that is the ribbon, the knife may be blunt or the paraffin is too soft.
(2) If the paraffin ribbon is cutting well, that is, the ribbon is not tearing nor crumbling then your dehydration, clearing and/or infiltration has fault. If your specimen stained longer than necessary in dehydrating agent or in the infiltrating oven or in the clearing agent especially in xylene, the specimen become hardened and brittle and it will be crumbling while cutting.
Thanks.
Akinloye A. J. PhD.
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I need histological images of paranasal sinus and tooth eruption for our new " Oral Histology" book project. We will mention provider at acknowledgements and under the image. Please do not hesitate me to contact me for details. #histology
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NT Fascicle 11 Chapter 1.pdf (arppress.org)
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I have measured breast tumor size using different modalities CESM and MRI. I have also the size on Histology.
I have a table of paired Samples Test, and the Sig. is .037 MRI and .523 for CESM when compared with the gold standard of histology. What does this mean??
Help xx
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The output table shows the degrees of freedom (df). The table also already tells you which modalities specifically differ. Please see my previous comments.
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I have scanned ovarian H&E slides and need to go through them and mark all of the follicles. There are several types of follicle (primordial, primary, secondary, antral, etc). I want to be able to quickly go through them and put a specific mark that indicates what type of follicle it is. The mark should be an overlay (not a permanent mark on the image) so that the original image is not altered. I want to be able to quantify the number of each follicle type at the end and keep track of everything. Open-source is preferable but a low-cost paid program would also be acceptable. I have messed around with several python based programs but none seem to fit the bill. Does anyone have anything that has worked well for you in the past?
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Hi Trent, have you done it? Automated the process?
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Hello!!
I am doing histopathological analysis of kidney sections with PAS stain. I want to quantify the staining intensity of the sections. can anyone explain what is the correct protocol for using the color deconvolution tool in ImageJ software?
Thanks in advance,
Sreyasi!
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Sreyasi Das . Once you have segmented or thresholded the regions you want to measure, you just have to "Set Measurements" to include Mean gray value (this is mean intensity), or integrated density, and you can then measure these regions.
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Hi there,
I am looking for the number of fibroblast in healthy human skin- dermal fibroblasts and I am not dividing them into subpopulations.
Does anyone have a good reference for this?
Optimal would be a value in [cells/cm^2 skin]. Maybe from histological countings or from isolations- How many fibroblasts can be isolated per cm^2?
It would be awesome if someone had an answer :-)
Kind regards
Miriam
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The mean number of cells dissociated per cm^2 biopsy after trypsin: EDTA digestion of a dermis-containing biopsy is 4.0 x10^6 cells/cm^2
Between 0.5% and 4% of the cells dissociated from a dermis-containing biopsy are human fibroblasts. For more information you may refer to the article attached below.
Hope this reference is helpful!
Best.
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I would create my plates (a group of histologic figures) in MS paint, then save in irfanview, where I resize and adjust to desired dpi. I couldn't group figures, label, adjust contrast, etc in irfanview. I thought there should be a software that does it all.
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Hi Davinson,
You might want to have a look at GIMP: https://www.gimp.org/
You can think of this as a free and open source Photoshop that runs on Windows, Mac and Linux. The website has a bunch of tutorials for image manipulation, setting up layers, adjusting DPI etc
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I am analyzing a batch of adipocyte tissues using Adiposoft which is a plugin for Fiji. The program is taking the area of the cells and giving me their Equivalent Diameters (D-eq). I read that "From the diameter, the average adipocyte volume and lipid content can be mathematically derived" (Galarraga et al. 2012).
Could someone tell me what formulas (and the definition of their variables) I can use to find the Lipid content and the volume of an Adipocyte using the D-eq and Area?
I also have the size of the image in microns and an estimate of how many cells are in the image based on what Adiposoft counted during its automated analysis.
Galarraga, M., Campión, J., Muñoz-Barrutia, A., Boqué, N., Moreno, H., Martínez, J. A., Milagro, F., & Ortiz-de-Solórzano, C. (2012). Adiposoft: automated software for the analysis of white adipose tissue cellularity in histological sections. Journal of lipid research, 53(12), 2791–2796. https://doi.org/10.1194/jlr.D023788
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Wait since its the equivalent diameter do I just calculate the equivalent volume using the volume of a sphere formula? -_-
V-eq=(1/6)*Pi*(D-eq)^3?
And is the equivalent volume then equal to the lipid content?
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How would you evaluate the glomerulosclerosis condition of thhese glomeruli (picture 1,2)?
These are glomeruli of rat kidneys. The rats were treated after administration of streptozotocin. Untreated rats had significantly hypertrophied glomeruli with eosinophilic deposits (picture 3). The size of glomeruli of the treated group was standard, however the majority of glomeruli had minimized or fast no Bowman´s space (picture 1,2) - is this actually a normal condition? Inspite of that, kidney function parameters of treated group were similar to the controll healthy group.
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These glomeruli look like they are within normal limits to me. These look like PAS stains, so you can't say that there are eosinophilic deposits (as this stain does not contain eosin). The basement membranes within glomeruli are staining for PAS (normal), but there do not appear to be significant increases in PAS-positive deposits, or clear loss of cellularity. You cannot conclude as to "hypertrophy" of glomeruli based on the diameter of a glomerulus on a histologic section, as this will depend on where the section goes through the glomerulus. I think you need to have your sections evaluated by a qualified pathologist.
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Hello, I am writing because in our lab we have been doing some histochemical stainings and thus using water-based mounting media such as Mowiol 4-88. We have been running into a number of difficulties in the mounting medium preparation starting from the powder (we have been following the standard protocol provided by the manufacturer, e.g. https://www.polysciences.com/media/pdf/technical-data-sheets/TDS-20777.pdf), but we struggle to get the powder in solution and the end product is way too watery to be used for mounting (we have resolved to use much less water/buffer than advised, but it's still not great). We have also encountered precipitation issues when keeping the Mowiol mounting medium stock at RT for some time.
Does anyone have any protocols/tips and tricks to share to prepare Mowiol 4-88 mounting medium or any analogous water-based mounting medium? Thank you so much!
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I recommend using the glycerogelatin (ready to use) from Sigma. It is easy to open a slide using warm water.
Regards,
@
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Hello everyone.
Recently I got these images from a small project I'm doing, but as you can see, there seems to be some things that resemble "crystals" in my samples, of course they only appear in the experimental group. I've tried looking for "lipid droplets", "inclusion bodies", "crystals", "protein accumulation", but so far I haven't been able to find something that resembles what I'm seeing here.
Can someone please give me a hand in trying to explain what is it that appears in these samples?
Thanks for your time and kindness.
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Elena Lebedeva Good afternoon! the samples were sent to a histopathological laboratory, the magnification was 20X. I apologize for the low sharpness, it's a photo I took with my cellphone to the computer screen at that moment (I wasn't at my lab nor on my pc), my mistake. We considered the artifact option, but a histopathologist told us the characteristics matched with salt deposits, although we don't know how to prove this.
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I consider purchasing a SLEE automatic microtome, but I never had an experience with it. Can anyone here give an opinion on the instrument and on their service?
Thanks a lot
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unless , no
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Hello,
I work with a mouse model that in response to ultraviolet radiation develop cutaneous squamous cell carcinoma
To grade the tumours, I excise them from the skin on the mouse’s back and leave them in formalin to be embedded in paraffin. The paraffin and sectioning part is done by our experienced technician who has worked with this type of tissue before.
But when the tissue has been embedded in paraffin and left for a few days before sectioning, the blocks started to look wrong (see attached picture). There are cracks in the paraffin and in some places (picture left) it almost looks like the tissue is rejecting/repelling the paraffin.
Nothing about the protocol has changed, we do a overnight program before the paraffin embedding, and the only thing that has varied (a bit, if any) is the increase in tumour size. The tissue in formalin feels like it should, and others have used the same program as us with no problems.
Our working hypothesis is that it might be the temperaure of the cooling plate where the slide are placed after the paraffin. On the last batch, we used -14 C and still observed cracks in the paraffin.
Does anyone have experience with this problem or know what it may be?
Thank you in advance.
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Another thing could be important. Take care that you use fresh solutions, especially when you embed a huge amout o tissue blocks. Best regards Ute
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I've been cryo-sectioning Xenopus oocytes and find these large internal branching structures in all of them (see attached image). I haven't been able to find a reference to it in the literature. I was tempted to say that it was ER, but I don't think it is. Does anyone have information on what this structure is?
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Hello Andrew Gipson,
In my opinion, it could be heterogeneous distribution of yolk platelets in the vegetal pole of Xenopus laevis oocytes. Two types of primordial yolk platelets (I and II) have been discriminated. After membrane fusion these precursors can be completely incorporated into the main body of existing platelets, numerous yolk crystals then merge and form one uniformly stratified core.
The yolk platelets ofXenopus laevis have been studied by thin-section and freeze-fracture electron microscopy to characterize the boundary membrane during yolk formation. Lipid droplets are tightly attached to the membrane at all developmental stages of yolk platelets. A direct connection of endoplasmic reticulum to the membranes of yolk platelets has not been observed. The proteinaceous yolk platelets tend to fracture along their periphery through the superficial layers.
For more detail see the following link:
Yolk organelles and their membranes during vitellogenesis ofXenopus oocytes. Roux's archives of developmental biology 198, 92–102 (1989).
Fluorescence lifetime imaging reveals heterogeneous functional distribution of eGFP expressed in Xenopus oocytes.
In this paper please see the figure 1B, similar to your attached image.
Best
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Occasionally when handling wetted picric acid or saturated solutions some solution can be transferred to gloves or a very small spillage (a few drops) might need wiping up with a tissue. I then dispose of these gloves and tissues in the bin where they can dry out. How dangerous are these dry residues in terms of explosion? What is the best practice in this case? Thanks.
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contact with even a small amount can cause an allergic reaction with symptoms such as skin redness, itching, rash and swelling.
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Hi there,
can anyone recommend a protocol for staining the mouse gamma-delta TCR in formaldyhyde-fixed, paraffin-embedded tissue ideally for immunofluorescence?
Thanks a lot!
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Hi... what is the best histology protocol for rat tissue (kidney, liver, heart, spleen)?
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You can review papers and follow the the staining method preferred.
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Hi all,
I'm trying to perform Masson trichrome on frozen tissue sections however my fuchsin staining is turning purple once I add methyl blue.
My protocol is as follows:
1. Fix in 4% PFA
2. Rinse in distilled water.
3. Stain nuclei using celestine blue-haemalum method.
4. Differentiate with 1% acid alcohol.
5. Wash in running tap water.
6. Stain with acid fuchsin solution for 5 minutes.
7. Rinse in distilled water.
8. Treat slides with phosphomolybdic acid solution for 15 minutes.
9. Drain and stain in methyl blue for 90 seconds.
10. Rinse in distilled water.
11. Treat in 1% acetic acid for 2 minutes.
12. Dehydrate through alcohol gradient and clear in xylene.
Any help would be appreciated.
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Good day, what type of tissues did you stain?
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Hi all
I am making histological slices of bones without decalcification. Normally I only clean the bones with several baths in boiling water with bleach, but I have not had the best results with this method. I wanted to know if anyone has been doing any additional methods to remove fat and organic remains from the bones. Any comment would be very helpful.
Thank you in advance!
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I think the protocol found in this article (in attached) may actually help you.
Best wishes,
Sabri
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It is kidney obtained from a rat with early diabetes mellitus type 1 (streptozotocin model).
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Hi
I think the eosinophilic material in Bowman's space is protein debris. I advise looking for signs of proteinuria such as hyaline casts. Indeed, occasionally within the renal tubules there appear to be small amounts of eosinophilic (proteinaceous) material...
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I will stain the osteoblast cells we planted on our hydrogels with Alizarin on the 14th and 21st days.
In the protocols I found, 2 grams of Alizarin Red S is dissolved in 100 ml of distilled water to prepare the dye. Unfortunately, I have just Alizarin (Mordant Red 11, 97% Sigma: 122777) on hand. Is it possible for me to implement the same protocol with this?
I have tried dissolving it in both water and DPBS but the dye sinks to the bottom and I get a feculent orangeish liquid. As far as I know, Alizarin Red S is not this color. Please help if you have any tips or experience on this.
I also noticed that when I used Alizarin Red S before, there was a lot of dye residue and it was difficult to see specific stains. No matter how many times I wash, I cannot remove the dye from the gels. I would be glad if you help.
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Hi Gulsah,
To get rid of precipitates, you need to filter Alizarin red once you make the solution.
Also, before staining, cells need to be fixed in 10% neutral buffered formalin (NBF) for an hour (or overnight at 4 degree) and then washed with PBS and stain for alizarin red for 30 min-1 hour. You can also buy a readymade solution of alizarin red (TMS-008). We use it all the time. You can dilute it 1:1 in distilled water and stain your cells, no need to even filter. If you want to use your own stain, dissolve it in water, I think you also need to put it at 37 degree for some time to dissolve properly and then filter to get rid of insoluble alizarin red particles.
Note: Fixation with NBF is very important, without it, your cells will not stain.
Disclaimer: I am not intended to promote any company product here, it is based on my personal experience.
Hope it helps!
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What is the best software for analyzing the histological pictures?
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Dear Dr. Arash
Image J is appropriate for analyzing the histological pictures. you can get it from below URL:
Also you can Run ImageJ in Browser. This option is new possibility about image J software.
yours truly
Please don't hesitate from contacting to me for more details about this question.
+989125032346
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I am running an IHC experiment on mouse brain tissue that has been virally infected and I am looking to track infection by using and optimizing antibodies that detect immune cells, such as astrocytes and microglia etc.. In this experiment, I am blocking with normal goat serum (NGS) in PBS prior to using a goat anti-ALDH1L1 that reacts with mouse as a primary, and a donkey anti-goat as a secondary. This is the second time I run this with the same conditions and I dont see signal - it seems like the secondary antibody is not binding to/ picking up anything (similar to my negative control under the microscope). I am sure that there is antigen is the tissue - I previously used rabbit anti-GFAP and the signal of astrocytes is very strong and visible, so I would expect very similar outcomes with anti-ALDH1L1. Could this be due to the use of a blocking serum from the same species and the primary antibody and not the secondary?
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You should avoid using blocking serum from the same host species as your primary antibody that is detected by a secondary antibody against the same species because immunoglobulins in the blocking serum will compete for the secondary binding.
In your case, you should avoid using goat serum for blocking if you are using a goat primary antibody with anti-goat secondary antibody.
Use serum that is from the same species as your secondary antibody. You could also use BSA for blocking.
Best.
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Im working with fresh apical stems and this organ is very soft. After embed in Cryomatrix or OCT, it is posible to obtain around 40% of sections with good morphology (even cambium cells). But in the other 60%, shrinkage happens specially in cambium cells. Troubles:
- Samples after dry suffer of cell shrinkage, specially the cambium cells
- Samples after ethanol wash (for laser microdissection), suffer an even more shrinkage.
How could I avoid shrinkage in my cells? 
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يمكنك مراجعة المختصين في هذا المجال
شكرا لكم
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Is there any technique associated to histology that allows to detect a mutation in a specific gene of a single cell of a certain tissue? (i dont need to know the type or detail of the mutation, only that there is one), assuming that the rest of the cells have a wild type gene
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الطفرات التي تحدث في الخلايا الجسدية تسمى طفرات جسدية، ولا يمكنها الانتقال إلى النسل عن طريق العمليات التكاثرية في الحيوانات.
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Hi, I would like to find a good anterograde tracer. Which is better, BDA or Fluoro-Ruby? Thanks a lot in advance!
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يمكن مراجعة المختصين
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I am using snap frozen muscle biopsies and am using a non-commercial antibody currently but would like to start buying a commercial antibody. 
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يمكن مراجعة المختصين
شكرا لكم
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For training a network I'm searching for a dataset of complex bio-images. I have seen some datasets for cell-classification, but these are usually simply amplitude-only samples, like these: http://imagej.net/Public_data_sets
Does anybody know any source of microscopic phase-images or does anybody have a set of complex images which could be used as a base?
I acquiered a small set using the quantitative Differential Phase Contrast Method provided by Lei Tian. These images are working quiet well :-)
Many thanks in advance!
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تجدها على النت يمكنك البحث
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I am trying to find any literature regarding the effects of diabetes on the amount of living PBMCs. Specifically using flow cytometry. In my own research i'm finding a difference between control and hyperglycemic groups.
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ممكن سؤال المختصين بذلك
شكرا لكم
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In tissue processing, xylene is toxic and difficult to regulate end point (especially manual processing). mineral oil - isopropanol combination can be used effectively. but what processor uses this method? also can the mineral oil be recycled?
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المعالج الدقيق أو المجهري أو الصغري (بالإنجليزية: microprocessor)‏ (يرمز له أحيانا ب µP)، ... وبذلك يستخدم المعالج الدقيق بالعديد من الوظائف،
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Dear colleagues,
I am currently looking for used but minimally working microscopy, histology and cytology equipment. Is there anyone who wishes to get rid of old things? E.g. microtomes, tissue processors, microscopes, etc?
Thanks.
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من البكتريا
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I will stain heart tissue slices with TTC in order to measure the infarct area. I read that the age of the heart after death is important factor for validity of the test. I wonder how long can I store the heart slices before TTC staining? What is the optimal time to do TTC staining after collecting the heart samples? 
Thank you.
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لا اعلم يمكن سؤال المختص في ذلك
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We perfuse our mice with PBS followed by 4% PFA, harvest brains and allow to fix overnight (20 hours) in 4% PFA before moving brains directly into 30% sucrose for 24-48 hours until tissue has sunk. Then the brains are frozen on dry ice into OCT and sectioned in a cryostat. The 20um sections are kept long term via free floating in a 24 well place immersed in our cryoprotectant recipe below:
STEP 1 Prepare PB (0.1 M phosphate buffer pH 7.2)
1. Prepare sodium phosphate dibasic stock (0.5 M Na2HPO4) by dissolving 35.5 g of sodium phosphate dibasic in a final volume of 500 mL of H2O.
Some crystallization will occur when the solution is stored at 4ºC. Warm on a hot plate and stir until the crystals dissolve.
2. Prepare sodium phosphate monobasic stock (0.5 M NaH2PO4) by dissolving 30 g of anhydrous sodium phosphate monobasic in a final volume of 500 mL of H2O. For NaH2PO4.H20, measure 34.5g.
3. Prepare 0.1 M sodium phosphate dibasic: Put 80 mL of sodium phosphate dibasic stock (0.5 M) from Step 1 in a beaker and add H2O to give a final volume of 400 mL.
4. Prepare 0.1 M sodium phosphate monobasic: Put 30 mL of sodium phosphate monobasic stock (0.5 M) from Step 2 in a beaker and add H2O to give a final volume of 150 mL.
5. Bring the 0.1 M sodium phosphate dibasic solution from Step 3 to pH 7.2 by adding as much as needed of the 0.1 M sodium phosphate monobasic solution from Step 4.
The resulting solution is 0.1 M phosphate buffer pH 7.2.
STEP 2 prepare Cryoprotectant (Use for Immunofluorescence)
To make 1000 ml cryoprotectant
• Sucrose ----------------------------- 300 g
• Polyvinyl-pyrrolidone (PVP-40) --- 10 g
• 0.1M PB ----------------------------- 500 ml
• Ethylene glycol --------------------- 300 ml
Add PVP-40 to 0.1M PB. Stir to dissolve. Slowly add the sucrose to dissolve, and then add the ethylene glycol and bring the final volume to 1000 ml with 0.1M PB. Store at -20°C.
I am looking to know how this cryoprotectant can affect tissue overtime for lipid based targets, specifically MBP? For our longer kept tissue ( > 1 year) we are having an unsucessful time staining for MBP via IF and the black gold II kit. I have suspected the cryoprotectant for a long time, seemingly the longer kept tissue doesn't stain as well; When i compared floating sections kept in cryoprotectant vs directly adhered sections, dried and then stained, the color outcomes for the black gold kit were very different. Directly adhered had more dark purple/blue/red while the tissue that had been exposed to cryoprotectant was more black/grey (diverging from expected color results but still identified tracks appropriately).
We have tissue that can't be recollected and i need to figure out how i can save the tissue currently in cryoprotectant for myelin staining. Neither IF myelin nor black gold II kit staining is working on this older tissue, which we should be seeing ample amounts of ( by WB analysis of mice collected from this group, myelin is ample) but staining will work on more newly collected tissue exposed to cryoprotectant.
Does anyone have any suggestion or publication about the effects of this type of cryoprotectant on targets post fixation? I was wondering if the direct immersion in 30% sucrose was an issue but again, this appears to be an "over time" problem for our sections.
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من خلالها نقل الدهون والفيتامينات الذوابة في الدهن بعد امتصاصها على شكل مستحلبات دهنية هي الدقائق الكيلوسية
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if medico-legal case comes with fractured tooth segment or avulsed tooth, then how to determine time period or time duration of injury/assault clinically or histologically?
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بحسب مرونة السن
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Dear fellows,
I am looking for an expert in lung histology to collaborate on one of my project.
The expected work is to analyze histology images (e.g H&E, Trichrome, IHC etc) I have obtained from lung tissue from different mice models and provide me with an accurate description, quantification, an proper figure panels of whatever phenotype he/she will observe. The researcher will of analyze all the samples blindly.
For this work the chosen collaborator will of course be one of the co-authors of the future publication using this data.
look forward to here from you.
Best,
Yair
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اعطني قليلا من الوقت حتى اجد من يساعدك
شكرا جزيلا
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In histological examination, l found the diferences in distibution of panceatic cells in the pancreatic islets in the camel and buffalo
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جُزَيراتُ البَنْكرياس أو جُزَيْرَات لاَنْغَرْهَانْس (بالإنجليزية: Islets of Langerhans)‏ هي عبارة عن مجموعات صغيرة من خلايا البنكرياس تظهر ... وتحتوي هذه الجزر على خمس أنواع من الخلايا
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I have observed that fovea are usually resistant & usually gets affected in very advance stages of serpiginous & serpiginous like choroiditis. Is this observation is just by chance or does it have some histological reasoning?
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اعتلال المشيمية و الشبكية المصلي المركزي هو حالة تتميز بتغيّر في نفاذية ... وعادة ولكن ليس بالضرورة يكون الانفصال في منطقة البقعة و النّقرة
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Guys, which is the best protocol according to Your experience to cut ultrathin sections of human embedded spermatozoa?
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الحيامن المنوية
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Surget A , Barron C . Histologie du grain de blé[J]. Industries Des Céréales, 2005.
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ان شاء الله تعالى
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Hi all,
I would like to visualise the cartilage wear on the surface of glenoid after friction test using a UV light (on macroscopic level). Could you please recommended me a florescent dye that I can use and won't affect the structural propertied of cartilage as I will be using the cartilage for histology after. 
Thanks in advance.
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لأن تمزُّق الغضروف الهلالي يحدث في غضروف لا يظهر على الأشعة السينية. لكن يُمكن للأشعة السينية المساعدة في استبعاد المشاكل الأخرى للركبة، والتي تتسبَّب في أعراض مشابهة.
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Any other detailed information about these cell lines would be highly useful to me.
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لا اعلم
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How to stain Chitin molecule in a tissue?
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قضيته واضحة
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A common way is to use AngioTool, but the thing is, I have to firstly pretreat the histology slides and delete all useless objects (eg. adjacent cells).
Is their some possible way to extract vessel skeletons?
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يمكنك مراجعة البحوث الخاصة بالاساتذة للحصول على الجواب
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Asthma is a chronic, obstructive disease;
In asthma we have hypersecretion of mucos ;the main component of mucos is mucin ; the main airway mucins are muc 5ac and muc 5b that are released from goblet cell and submucosal glands ,respectively.
Asthma characterized by some changes, like: thickening of the lamina reticularis, epithelial shedding, subepithelial fibrosis, inflammatory cell infiltration, goblet cell hyperplasia, myofibroblast proliferation, smooth muscle hyperplasia and hypertrophy, and neovascularization of the airway wall
According to the findings ; increased amount of the muc5ac and decreased amount of muc5b is observed.
Goblet cell hyperplasia can cause more expression of muc5 ac but there is no evidence for the reason of decreased amounts of muc5b .I'm looking toward this decreasing reasons.
I will be thankful if you share your ideas with me .
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يمكنك مراجعة اهل الاختصاص
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I find different volumes and different number of cells to inject Wistar rats. I want to inject the maximal volume in order to have a good-size tumor.
I injected 200ul by accident and the rat died few minutes later.
Injecting 20ul didn't cause a clinical changes and I'm waiting for the histology outcome...
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يمكنك مراجعة بحثي للحصول على الاجابة
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I am studying the histology of mammary glands of lactating Wistar rats. I need to differentiate glands with higher milk secretion from the glands with lesser or no milk secretion. What are the fundamental differences in gland's histology in this case?
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لا اعلم يمكن مراجعة المختصين
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Spherical molds to be used to embed tissue pieces to achieve isotropic orientation, as in the iSector technique for stereology.
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ليس لدي اقتراح ولكن يمكن الحصول عليها اثناء البحث
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Spinal cord histology
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الفرق الرئيسي بين الفقاريات والحبليات هو ذلك الفقاريات هي فرع رئيسي من الحبليات التي لها عمود فقري بينما الحبليات هي حيوانات متطورة للغاية تمتلك notochord، أ الحبل العصبي الظهري, الشقوق البلعومية، و endostyle، وما بعد الشرج ذيل خلال فترة من حياتهم.
الفقاريات هي مجموعة رئيسية من الحبليات فيما يتعلق بعدد الأنواع والتطور التطوري والعديد من الجوانب الأخرى أيضًا
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histological place of it in cattle
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وتقع على جانبي الحلق هو الاسم الذي يطلق على كتلة الأنسجة اللمفاوية.
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I'm working on DMBA induced breast cancer in mice. What should be the histological, molecular or some other tests needs to perform to establish the anticancer and apoptotic activity of the natural product against DMBA induced breast cancer in mice?
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اسباب كثيرة ينبغي عليك البحث عنها
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Histologically cementum is devoid of nerve endings
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غير واضح الرجاء تبديل صيغة السؤال
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All,
I am looking to buy a tissue microarray device for the histology core. I have never used TMA device before so I need expert advice as what would be the best option. I am considering the automatic one which is accompanied by computer software program.
What should be the maximum capacity of blocks 5 or 10?
how many core/punch size should I consider (considering it will be used in histology core)?
Any other aspect you think would be important to consider when buying this pricey equipment?
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السؤال غير واضح
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I'd like to determine microvessel dimensions in various tumor groups and am looking for an online database to draw pathology slides from.
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نعم ولكن تحتاج الى عملية بحث واسعة وكبيرة للحصول عليه
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I have a patient suffering from massive cervical lymphadenopathy, histological diagnosis as Rosai–Dorfman disease. Though it is spontaneous remission, i gave him steroid,but not significantly improvement. I like to know the chemotherapy like histiocytosis or radiotherapy.
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علاج كثرة المنسجات
على الرغم من أن كثرة المنسجات ليس مرضا خبيثا, إلا أن معالجة المرضى تتم، في معظم الحالات، في قسم الأورام (Oncology), وذلك، أساسا، بسبب نوع العلاج الذي يشمل، في أحيان كثيرة، أدوية مضادة للسرطان. وفقط في حالات نادرة تتم المعالجة بواسطة الأشعة (Radiotherapy). يعطى الستيرويد (Steroid) كعلاج موضعي أو عن طريق الفم، وخاصة عندما يكون الهيكل العظمي مصابا بهذا المرض. بعض المرضى يتعافون من هذا المرض تلقائيا (دون تلقي أي علاج خارجي).حدوث مشترك لداء كثرة المنسجات لخلايا لانجرهانس ومرض روساي دورفمان: علاقة محتملة لاثنين من اضطرابات المنسجات في حالات نادرة
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I am new at cell/tissue/explant culture and I am trying to work with skin explants of marine fish. The goal is to study effects of bacterial invasions and possible risk factors to increase this invasion. To do this, we would like to keep an explant viable for a short period of time (1-2 days) without radical changes in histological parameters. Any suggestions about important factors we need to keep into account? Which media would you suggest? Which pH should the media have? Can we add salts to the media to increase the PSU without endangering the survival of the explant? Thank you very much for all help!
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حقق الاستزراع السمكي فى السنوات الأخيرة طفرة كبيره فى مصر فقد بلغ أجمالي الإنتاج من الاستزراع السمكي سنة 2007 حوالي ( 635 ) ألف طن ومعظمه من أسماك المياه العذبة ويمثل حوالي 63% من الإنتاج الإجمالي من المصادر المختلفة للأسماك فى مصر , على الرغم من ذلك مازال الإنتاج السمكي لا يغطى احتياجاتنا الغذائية حيث استوردت مصر فى سنة 2007 حوالي 259 آلف طن من الأسماك بقيمة إجمالية حوالى 1.2 مليون جنيه ويرجع ذلك لقلة الأنتاج من المصايد الطبيعية بسبب التلوث وأرتفاع جهد الصيد لذلك فالحل الأمثل هو اللجوء للأستزراع السمكي عموماً والبحري خصوصًا حيث إن مقومات هذا النوع من الاستزراع موجودة في مصر (مياه – أراضي – تكنولوجيا).
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We have obtained some electron micrographs showing what appears to be developmental stages of some type of small eukaryotic organism colonizing/parasitizing the tissues of mammalian hosts. Samples tested included sterile deep needle aspirate of subcutaneous nodules, filtered lysed whole blood, and urine sediment. The hosts may have a unique genetic defect allowing them to become infected with eukaryotic parasites, or, the putative parasite may have efficient strategies for suppressing the hosts immune response.
We are curious about the identity of this possibly novel organism. None of us in the research group have more than basic knowledge of invertebrate taxonomy, but based on the presence of organized calcified “tube or shell”-like structures, we are hypothesizing that it may be some type of polychaete or mollusk? The opinion or thoughts of anyone skilled in such classification would be very appreciated. Thanks!
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ربما يوجد ذلك
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I am culturing skin cells in 3D ECM matrix gel to develop 3D skin model. It is being developed by embedding dermal fibroblasts in 3D matrix gel and seeding of Hacat cells on the top of the dermal matrix. Total duration of the culture is 10 days.
Further, I fix the gel with 4%PFA (at 37 deg C for 30 mins) and maintain it in 30% sucrose overnight at 4 deg Celsius prior to embedding in OCT and ultimately cryosectioning.
After embedding in OCT, I snap the freeze the sample in liquid nitrogen and keep it at -20 deg C for 10-15 mins and then -80 deg C for 1-2 h. Further, I cryosection the sample using Leica cryotome to get 40 micron size cryosections (please refer to the attached images).
However, I notice that there is no uniformity in gel/culture distribution throughout the section. Also the gel seems to be cut off from the membrane support (black, dark colored thread like part in the attached images- acts as a support for gel-embedded culture) at many places. Thus, presently the section looks completely distorted and I am not able to achieve skin orientation properly.
1) What changes are needed in gel processing and cryosectioning?
2) How should I protect Hacat epidermal layer (seeded on the top of dermal matrix) from getting disturbed/distorted while lifting the gel for further processing?
Please help regarding the same.
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السؤال غير واضح للاسف
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New transplant programme starting in a small African nation, but they will need support and training in the examination of biopsies. A digital 'whole slide' scanner would allow virtual biopsy images to be shared with expertise in the UK. If anyone knows of a machine not being used and willing to donate, please get in touch!
Best wishes,
Benedict Phillips
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نعم يوجد
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I'm searching for proper histomorphometric parameters to analyze the differences between healthy and osteoporotic bone on histological sections (formalin-fixed, paraffin-embedded) with a particular interest in cortical bone.
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بالتاكيد قاموا بذلك
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for histological study especially vasculature quantification, we need to isolate the chorioallaltoic membrane.
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نعم يوجد فيديو يشرح ذلك
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Hi everyone.
Sometime, I need to save time, so I would like to know if it is possible to practice the IHC protocol on histology slides stained with MGG.
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لااعتقد
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Clearing-enhanced 3D is a medium used in histology and is compatible with most immunostaining methods including multiplexing IHC. It can also be used to increase the transparency of full organs. Nevertheless, it may take a long time for the incubation process to take place. My question is how can you overcome some of the limitations associated with the use of Ce3D clearing medium?
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يجب مراجعة المختصين في هذا المجال للحصول على الاجابة النموذجية
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Hi
Come August I'll be doing a presentation on a topic related to Histology for the lab I work for, and I chose to talk about plants. We work with all kinds of surgical specimens, so I wanted to talk about a histo field that's different from ours. It's also an opportunity to talk about a bit of botany.
I was wondering if anyone here knew of any good articles/books to look into that talk about current methods on how plants are prepared? Particularly, readings that discuss plant fixation, processing, instrumentation/microtomy. Emphasis on paraffin-embedded tissue, too.
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هناك نوعان رئيسيان من الأنسجة النباتية هما الأنسجة الإنشائية والدائمة. النسيج الإنشائي ، النسيج الأولي للنمو في النباتات ، قادر على التجدد الذاتي
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I'm a medical student with limited exposure to histological methods and would appreciate any help that can be offered.
Warm regards,
Shai
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لام الجافية هي غشاء سميك مصنوع من نسيج ضام كثيف غير منتظم يحيط بالدماغ والنخاع الشوكي . إنها الطبقة الخارجية من طبقات الغشاء الثلاث
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Is only imaging diagnosis sufficient before TACE?
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تأكيد استخدام الخزعة لتشخيص أورام الأنسجة الرابطة (DT) هو إلزامي وينبغي إنتظار حتمية الفحص النسيجي قبل إتخاذ أي خطوات أخرى للعلاج
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View histology svs files on a mac, free to download software.
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يمكن سؤال المتخصص
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To detect by WB, I would have to isolate the nucleus fraction, right? But I did whole protein isolation. Has anyone detected ATF4 or ATF6 by histology?
Thank you
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integrated stress response
ATF4 is a basic leucine zipper (bZIP) stress-responsive transcription factor that belongs to the CREB/ATF family of transcription factors that are able to homodimerize o
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Hi, I am trying to create a histology based finite element model of human soft tissue. In order to model the viscoelasticity at the cellular level, I need to know the dynamic and bulk viscosity of the cell nucleus, cytoplasm, and extracellular matrix (ECM). I have looked through the literature. But I have not found any exact results. Could you please provide any information regarding this? Thank you.
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لاللزوجة الديناميكية (أحيانا تسمى أيضا اللزوجة المطلقة ) هو مشترك بين ... قد تظهر المواد الصلبة اللزجة اللزوجة كل من لزوجة القص واللزوجة السائبة
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I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can anyone weigh in on the quality and their experience? TIA
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نعم يوجد بعض الاشخاص لديهم خبرة في هذا المجال
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I want method in estimating the thickness of the granular cells by histology.
Is estimating the thickness of the granular cells of great value ?
Thanks
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بقى سلائف الخلايا الحبيبية في منطقة تحت حبيبية تصبح أرق تدريجياً مع نمو التلفيف المسنن، ولكن يتم الاحتفاظ بهذه الخلايا السليفة في الفئران البالغة
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Trying to find out what tissue and nerve innervation changes occur in the rectum after diabetes. Specifically changes that might influence how the rectum contracts to induce defecation.
Any current data or general consensus or references on this subject - changes of different nerve innervation in the rectum after diabetes would be helpful.
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لا علم لنا بذلك
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Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with augmented malaria infections. Thanks. Patrick. I need antibodies that react to mice hepatocytes. And also mouse cd8 and Tcells.
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ممكن ذلك من خلال البحث عن احد يساعدك في ذلك
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I have a set of responses from students: "What did you like best during this week?"