Histological Techniques - Science topic

New histological techniques are needed to examine protein distribution in human tissues, which can reveal cell shape and disease pathology connections. Spatial proteomics has changed the study of tumor microenvironments by identifying spatial relationships of immunomodulatory cells and proteins and contributing to the discovery of new cancer immuno...

Tissue segmentation of histology whole-slide images (WSI) remains a critical task in automated digital pathology workflows for both accurate disease diagnosis and deep phenotyping for research purposes. This is especially challenging when the tissue structure of biospecimens is relatively porous and heterogeneous, such as for atherosclerotic plaque...

Background Peptic ulcer disease (PUD) is a multi-cause illness with an unknown role for gastric flora and metabolism in its pathogenesis. In order to further understand the pathogenesis of gastric flora and metabolism in PUD, this study used histological techniques to analyze the microbiome and metabolome of gastric biopsy tissue. In this paper, ou...

Introduction: Crenosoma vulpis and Eucoleus aerophilus are widely distributed lungworms infecting carnivores, mainly red foxes, and are localized in the trachea, bronchi and bronchioles. There are no reports from Africa regarding the presence of C. vulpis. The aim of the present study was to report a co-infection with C. vulpis and E. aerophilus i...

Detailed histological analyses are desirable for zebrafish mutants that are models for human skeletal diseases, but traditional histological techniques are limited to two-dimensional thin sections with orientations highly dependent on careful sample preparation. On the other hand, techniques that provide three-dimensional (3D) datasets including µC...

Objectives To investigate the extension of experimentally induced peri-implantitis lesions under various antiresorptive and antiangiogenic medications. Material and methods Fourty-eight albino rats had randomly received the following medications (dual application, n = 8 each): (1) amino-bisphosphonate (zoledronate) (Zo), (2) RANKL inhibitor (denos...

Twenty female rats and five male, eight-week-old inbred, All experimental rats taken were weighing 175-225 grams. The female rats were paired up with male rats and inspected for a vaginal plug at an early time of day. All-female rats were subdivided: into the control and the experimental and treatment (were divided into three subgroups according to...

Histological techniques are a traditional and essential tool for adequately comprehending cellular morphology and physiology in several scientific fields, including ecotoxicological studies. Although bioassays generally recommend using small test organisms, based on advantages such as low maintenance costs and the possibility of a higher number of...

Although the microanatomy of Meckel’s cave (MC) has been well studied, there are still controversies regarding the meningeal architecture of the space. Moreover, there are only general mentions of the arachnoid granulations near MC in just a few sources. This study is aimed at determining the frequency, location, and anatomical variability of the m...

The development of an ideal vascular prosthesis represents an important challenge in terms of the treatment of cardiovascular diseases with respect to which new materials are being considered that have produced promising results following testing in animal models. This study focuses on nanofibrous polycaprolactone-based grafts assessed by means of...

Mass spectrometry imaging (MSI) is a powerful tool that can be used to simultaneously investigate the spatial distribution of different molecules in samples. However, it is difficult to comprehensively analyze complex biological systems with only a single analytical technique due to different analytical properties and application limitations. There...

Background: Becker muscular dystrophy (BMD) is a genetic neuromuscular disease of growing importance caused by in-frame, partial loss-of-function mutations in the dystrophin (DMD) gene. BMD presents with reduced severity compared with Duchenne muscular dystrophy (DMD), the allelic disorder of complete dystrophin deficiency. Significant therapeutic...

Butlleti de la Societat Catalana d'Herpetologia. 2022. 29:44-55. (In Catalan Language). Between the years 2021 and 2022, a health surveillance has been carried out to detect the 15 main amphibian pathogens of which there is evidence in European amphibians in the Network of Parks of the Provincial Council (Diputació) of Barcelona: Park og Garraf, P...

Histology is a necessary technique for analysing the inner structure of a tissue. The histological observation of a tissue culture explant can be used for characterising the somatic embryogenesis processes. With the use of this technique, it is possible to evaluate changes in the explant, changes and the cellular origin of calli from beginning till...

Se han descrito áreas alopécicas en la zona lateral y medial del metatarso de los camélidos sudamericanos, y se les ha denominado «glándulas metatarsales», análogas a las presentes en los cérvidos. En este estudio, se colectaron muestras de tejido de la región metatarsal de 8 especímenes (6 alpacas adultas y dos fetos [5 y 8 meses]) en el camal de...

Ultrasound localization microscopy (ULM) is a recent advancement in ultrasound imaging that uses microbubble contrast agents to yield vascular images that break the classical diffraction limit on spatial resolution. Current approaches cannot image blood flow at the tissue perfusion level since they rely solely on differences in velocity to separate...

Duchenne muscular dystrophy (DMD) is a progressive disease caused by the loss of function of the protein dystrophin. This protein contributes to the stabilisation of striated cells during contraction, as it anchors the cytoskeleton with components of the extracellular matrix through the dystrophin-associated protein complex (DAPC). Moreover, absenc...

Xylem cells are responsible for water transport and mechanical support in most plants. Since vines depend upon other plants for mechanical support, the main responsibility of xylem cells in vines is primarily water transport and not mechanical support. The purpose of the current study was to study xylem characteristics in stems of five species of A...

Background During aneurysm microsurgery, the aneurysmal sac is excluded from circulation by placing one or more clips at the base of the aneurysm. In some cases of complex aneurysms or subarachnoid hemorrhage history, transient clipping before definitive clipping is necessary. The closing force of the transient clip is less than the permanent clip;...

Wood properties and agronomic traits associated with fast growth and frost tolerance make Eucalyptus nitens a valuable forest alternative. However, the rapid age-related decline in the adventitious root (AR) formation (herein, meaning induction, initiation, and expression stages) limits its propagation. We analyzed transcriptomic profile variation...

En los últimos años las estrategias reproductivas para invertebrados de aguas profundas fueron en aumento. Varios autores han indicado la proporción inusual de especies bentónicas con desarrollo protegido de diferentes taxas en el Océano Austral, en particular en el Atlántico sudoccidental, incluyendo aguas profundas aledañas. En el siguiente traba...

El tomate de cáscara (Physalis) es reconocido como un ingrediente principal en la cocina mexicana. En México se cultiva Physalis angulata y P. philadelphica. El fruto es una baya que está encerrada por un cáliz acrescente conocido como cáscara, lo que le da su rasgo distintivo. Además de las dos especies más cultivadas, existen parientes silvestres...

Purpose: The purpose of this study was to determine whether rodent lacrimal glands (LGs) represent a suitable surrogate for human tissue in bio-engineering research, we undertook a meticulous histological and histochemical comparison of these two tissues. Methods: Histological techniques and immunohistochemistry were used to compare the structur...

Background: The evaluation of bone remodelling and dental root resorption can be performed by histological techniques or micro-computed tomography (micro-CT). The present study aimed to evaluate the relationship between these two procedures in the context of cleft repair in a rat model. Methods: The reconstructed maxillae and the orthodontically-m...

Lithraea molleoides (Vell.) Engl. es un árbol resinoso, popularmente llamado “aruera” o “molle de beber”, causante de alergias a ciertos individuos sensibilizados, lo que se atribuye a alqu(en)il-catecoles presentes en hojas y corteza. Habita en Bolivia, Brasil, Paraguay, Uruguay, y Argentina, desarrollándose en bosques secundarios de las provincia...

Analysing the composition and organisation of the fibrous capsule formed as a result of the Foreign Body Response (FBR) to medical devices, is imperative for medical device improvement and biocompatibility. Typically, analysis is performed using histological techniques which often involve random sampling strategies. This method is excellent for acq...

Background The buccinator muscle derives from the mesenchyme of the second pharyngeal arch. In adults, it has a quadrilateral shape, occupying the deepest part of the cheek region. Its function is complex, being active during swallowing, chewing, and sucking. To our knowledge, there are no studies that have specifically analyzed the relationship of...

One of the main health concerns of diabetes is testicular dysfunction and impairment of reproductive function and sperm quality which can cause male infertility. kisspeptin is a hypothalamic neuropeptide hormone that is involved in the regulation of energy metabolism, gonadotrophin-releasing hormone (GnRH), and reproductive function. In the present...

Background: The genus Coryphantha has the second largest number of species of the tribe Cacteae-Cactaceae. Morphologically, it has been reported that the stem presents mainly globose, ovoid and cylindric variations. However, at the anatomical level, descriptions are scarce and focused on particular tissues (epidermis or xylem). The aim of this work...

Space and time coherent mapping (STCM) is a technology developed in our laboratory for improved matrix-assisted laser desorption ionization (MALDI) time of flight (TOF) imaging mass spectrometry (IMS). STCM excels in high spatial resolutions, which probe-based scanning methods cannot attain in conventional MALDI IMS. By replacing a scanning probe w...

Aim. To identify the structural foundations of the pathogenesis of arterial hypertension and kidney disease associated with a high-fat, high-carbohydrate diet and age. Materials and methods . The study was carried out on male Wistar rats aged 60 and 450 days. The animals were divided into 4 groups: group 1 (n = 14) – intact rats (60 days old) fed w...

The study of endemic fish species is of great importance, considering that extensive knowledge is a prerequisite for adequate protection and conservation. This study investigated the histology of the digestive tract of the steno-endemic fish Cobitis herzegoviniensis Buj & Šanda 2014, from Mostarsko Blato (Neretva River basin, Bosnia and Herzegovina...

Background: Silicosis is a severe pulmonary disease caused by inhaling dust containing crystalline silica. The progression of silicosis to pulmonary fibrosis is usually unavoidable. Recent studies have revealed positivity for the overexpression of C-X-C chemokine receptor type 4 (CXCR4) in pulmonary fibrosis and shown that the CXCR4 inhibitor AMD3...

Nasal conchae increase the surface area of these cavities, thus providing for rapid warming and hu-midification of air and by regulating nasal airflow, play a role in immune surveillance because they are the first intranasal structures to come into contact with outside air. The present study aimed to depict the onset time of the first appearance an...

Papillary thyroid carcinoma (PTC) comprises 80% of well-differentiated thyroid carcinomas. PTC progression is determined by a variety of biological markers, some of which are connected to the activities of Tumor-Associated Macrophage (TAM). TAM activity is difficult to observe using simply traditional histology techniques. Matrix Metalloproteinase...

Bisphenol A (BPA), one of the endocrine disrupting chemicals, is the object of great concern because of its widespread use throughout the world. In this study, it was aimed to determine the effects of in ovo administrated BPA on the bursa of Fabricius and percentage of acid phosphatase positive lymphocyte in peripheral blood by means of histologica...

Neste trabalho, objetivou-se utilizar exsudato resinoso de uma planta típica do Nordeste brasileiro, a goma de cajueiro (Anacardium occidentale L.) como alternativa de meio de montagem na técnica histológica, economicamente viável e ambientalmente saudável. A goma do cajueiro foi processada de acordo com o método adaptado de Rinaudo e Milas. A leit...

This study was carried out to see the regional and age-related histomorphological variations of the sebaceous gland of the Black Bengal goat. Study population was divided into pre-pubertal, pubertal and adult age groups. Tissue samples were collected from four anatomical regions; neck, thorax, abdomen and loin each having three sub-regions namely d...

S6.5 Efforts of improving the management of mycetoma: working towards the 2030 goals, September 22, 2022, 4:45 PM - 6:15 PM Background: Mycetoma is a chronic granulomatous inflammatory disease that is caused either by bacteria or fungi. The diagnosis of species is important to guide the therapeutic management of patients particularly for white and...

Antecedentes y Objetivos: En México solo existen dos publicaciones referentes a la madera de plantas distribuidas en selva baja caducifolia. En Puebla, este tipo de vegetación ocupa el segundo lugar en extensión geográfica. Sin embargo, no se han analizado especies típicas de esta zona. Por ello, los objetivos de este trabajo fueron describir la an...

Current understanding of cephalopod digestive tract physiology is based on relatively “old” literature and a “mosaic of data” from multiple species. To provide a background to the discussion of methodologies for investigating physiology we first review the anatomy of the cephalopod digestive tract with a focus on Octopus vulgaris , highlighting str...

Beverton & Holt's (1957) functional definition of maturity in fish (L50), as being the size class in which 50% of individuals begin producing gametes in some proportion to body weight, is widely used in assessment models to estimate spawning stock biomass, and manage the minimum size of capture. Standardized histological techniques for estimating L...

Objective This study explores the validity of Paget’s disease of bone (PDB) reported in unpublished skeletal reports, based on macroscopic analysis alone. Materials The high prevalence of ‘suspected’ Paget’s disease (10.7%) in an early modern sample from St John’s the Evangelist Church in Redhill, Surrey is reassessed. Methods Signs of PDB were e...

Histological techniques consist of traditional and very important tools for a better understanding of cellular morphology and physiology in ecotoxicological studies. Despite bioassays generally recommending the use of small test organisms, based on some advantages as their low maintenance costs and large replicates’ number, the histological process...

Aim Appendicitis is one of the most common surgical pathologies amongst children, with approximately 80% in the UK admitted and managed in district general hospitals. The Royal college of Surgeons paediatric emergency appendicectomy commissioning guidelines state a negative appendicectomy rate should be <15% and <1% should be admitted following a p...

En la Avicultura industrial, por las restricciones legislativas del uso de antibióticos promotores del crecimiento, se requieren alternativas naturales, como los probióticos. Bacillus amyloliquefaciens (BA) es un probiótico productor de enzimas que aumentan la digestibilidad y absorción de los nutrientes y metabolitos y la inmunidad. En nuestro lab...

Introducción: Las larvas de Galleria mellonella están siendo utilizadas como modelo animal para estudiar enfermedades infecciosas. Para evaluar microscópicamente las interacciones huésped-patógeno in vivo se requiere un correcto procesamiento histológico de las larvas que permita obtener buenos cortes para valorar los cambios que se generan durante...

Leptopanchax opalescens is a critically endangered small annual fish. Reproductive traits of this species were studied to improve our understanding of the strategies that facilitate the occupation of temporary wetlands. We compiled egg diameter and maximum total length data from 136 neotropical killifishes (Rivulidae) to establish comparisons betwe...

Helicobacter pylori infection is widespread worldwide, with more than a half of the world population infected. H. pylori antibiotic-resistant strains and non-compliance to therapy are the major causes of H. pylori eradication failure. The search for new therapies based on plant extracts is a scientific interest field. The present study was conducte...

Histological examination of targets in regions of interest in histological sections is one of the most frequently used tools in biomedical research. However, it is a technical challenge to secure a multitarget section for inspection of the structure’s mutual relationship of targets or a longitudinally filamentous- or tubular-formed tissue section f...

The stressful environment in Pranburi River estuary of Thailand severely impacts the aquatic ecosystem and may be causing reproductive alteration in the estuarine fishes living in this area, although there is little evidence of such deteriorated reproductive system. Thus, we aim to investigate the reproductive system, in particular the ovarian alte...

Background According to our acknowledgment this is the first full anatomical description of the studied laryngeal mound of the Egyptian Cattle Egret (Bubulcus ibis, Linnaeus, 1758). This study was obtained with the aid of scanning electron microscopy (SEM) and histological techniques. Heads of ten healthy adult male Egyptian cattle egrets were used...

Recent investigations of COVID-19 have largely focused on the effects of this novel virus on the vital organs in order to efficiently assist individuals who have recovered from the disease. In the present study we used hippocampal tissue samples extracted from people who died after COVID-19. Utilizing histological techniques to analyze glial and ne...

The present study aimed at investigating the histological structures of the vallate papillae of the tongue of the dromedary camel. Samples were taken from the tongues of 5 healthy adult dromedary camels, then processed by standard routine histological technique. The vallate papillae were broad, encircled by a distinctive sulcus, and exhibited conca...

Gastroscopic biopsy provides the only effective method for gastric cancer diagnosis, but the gold standard histopathology is time-consuming and incompatible with gastroscopy. Conventional stimulated Raman scattering (SRS) microscopy has shown promise in label-free diagnosis on human tissues, yet it requires the tuning of picosecond lasers to achiev...

Microsatellite instability (MSI) is a clinically important characteristic of colorectal cancer. Standard diagnosis of MSI is performed via genetic analyses, however these tests are not always included in routine care. Histopathology whole-slide images (WSIs) are the gold-standard for colorectal cancer diagnosis and are routinely collected. This stu...

Introduction: The African Grasscutter (Thryonomys swinderianus) is one of the few current animal models being considered for research. The animal has shown much research potential due to its similarities to other rodents. Aim/Objective: The study aimed to provide baseline data on the types of dopaminergic neurons in the cervical spinal cord of the...

Background: A medieval mummy known as the Blessed Antonio (Patrizi) is held in the church of Saints Peter and Paul at Monticiano, Sienna, central Italy.Objectives: The aim of our investigation was to complete a biological profile of the subject, as well as to assess the impact of deterioration to the concerned remains.Methods: As a follow-up of our...

The study was performed on 48 female Wistar outbred rats weighing 180-220 g, 6-8 weeks old. The animals were kept under standard controlled laboratory vivarium conditions. Animals were divided into 2 groups (n=24) - control and experimental. An experimental burn was modeled in each group according to a standardized method for studying the dermatotr...

Temporal focusing-based multiphoton excitation microscopy (TFMPEM) just provides the advantage of widefield optical sectioning ability with axial resolution of several micrometers. However, under the plane excitation, the photons emitted from the molecules in turbid tissues undergo scattering, resulting in complicated background noise and an impair...

Standardised and high-throughput methods have been developed for the production and experimental handling of some 3D in vitro models. However, adapted analytical tools are still missing for scientists and researchers to fully exploit the potential of complex cellular models in pre-clinical drug testing and precision medicine. Histology is the estab...

In the study of cerebral cavernous malformations (CCMs), the quantification of lesion burden is the main parameter for evaluation of disease severity and efficacy of drugs. We describe a reliable and cost-effective protocol to evaluate the number and the size of vascular malformations in the murine brain. This approach is based on histology and con...