High-Performance Liquid Chromatography - Science method

液相色谱技术（HPLC）在化工产品的分析领域得到了广泛应用，它在石油化工、生命科学、环境保 护、医药和食品安全等多个领域都展现出了高效、迅速和准确的分离分析能力，起到了至关重要的作用。本项研究通 过全面回顾高效液相色谱技术在石油化工原料和产品分离分析方面的最新研究进展，深入探讨了HPLC在分离难以挥 发的化合物、复杂混合物以及多环芳烃方面的分离效能。同时，分析了液相色谱技术与其他分析技术的联用，以及在 新产品研发中的应用策略。本研究表明液相色谱技术应用于化工产品分析有显著潜力与前景，经过持续技术创新与应 用策略优化，可望在化工产品分析方面发挥更大作用。

High-performance liquid chromatography (HPLC) plays a crucial role in purifying peptides and proteins and monitoring their reactions. Peptide hydrazides are widely employed intermediates in modern peptide/protein chemistry. However, they often exhibit peak tailing during HPLC purification and analysis. Herein, we describe a dialkoxybenzyl-linker ap...

In this study, the effects of physicochemical and biochemical digestion conditions on β-arbutin stability and bioaccessibility were investigated. β-Arbutin, at doses that correspond to its natural occurrence in plant materials (i.e., 1–100 mg), was subjected to gastric and intestinal electrolyte treatments (application of physicochemical factors) o...

Enteral nutrition (EN) formulas are necessary for critically ill patients to meet their metabolic requirements. Polyamines (putrescine, spermidine, and spermine) are crucial dietary components, with spermidine being particularly interesting due to its multiple proposed benefits. The requirements for and intake of polyamines have yet to be investiga...

Jatropha curcas is a widely spread plant, with its seeds being rich in oil. Accurate quantification of the toxic phorbol esters (PEs) present in Jatropha curcas seed oil (JCSO) is crucial for related research endeavors. The commonly used high-performance chromatography (HPLC) method for PEs has constraints such as multi-stage extraction of PEs and...

Hairy roots (HRs) produced by Agrobacterium rhizogenes infection on plants are an excellent system for producing economically valuable metabolites. Oldenlandia umbellata L. is a source of an important textile dye, anthraquinone (AQ). The current study focuses on optimizing different parameters for the successful transformation of O. umbellata by fo...

En este estudio se ejecutó la gasificación de celulosa en agua a temperatura supercrítica (SCWG) usando un reactor PFR. Se valoraron tres temperaturas de reacción: 372 °C, 374 °C y 376 °C durante 15 minutos. Los productos se analizaron mediante gravimetría, cromatografía líquida HPLC y cromatografía de gases, permitiendo la determinación de los par...

Sulfaquinoxaline (SQX) is widely utilized in aquaculture and animal husbandry due to its broad antimicrobial spectrum and low cost. However, it is difficult to degrade, and there are relevant residues in the aquatic environment, which could be harmful to both the ecological environment and human health. As a new recognition molecule, the aptamer ca...

A recent development in non-aqueous two-step (disperse and reactive) dyeing technology, minimizes water usage, eliminates the need for salt, and avoids the production of wastewater during the dyeing of polyester/cotton blend fabrics. However, an issue of color staining on cotton fibers during the disperse dyeing process has been observed. To addres...

Background Due to its exceptional effectiveness, clozapine (CLO), whose metabolite is norclozapine (NCLO), is a drug of choice in the management of treatment-resistant schizophrenia. The purpose of this study was to assess the factors modifying the CLO/NCLO ratio (CNR). Methods A total of 446 blood samples (233 of which were drawn from females and...

The increasing use of plastics globally has generated serious environmental and human health problems, particularly in the agricultural sector where low-density polyethylene (LDPE) and other plastics are widely used. Due to its low recycling rate and slow degradation process, LDPE is a major source of pollution. This paper addresses the problem of...

Objective: To determine Beyond Use Date (BUD) of cetirizine hydrochloride syrup preparation by analyzing cetirizine concentrations using High-Performance Liquid Chromatography (HPLC) with an Ultraviolet (UV) detector. Methods: The analysis was carried out using mobile phase acetonitrile-phosphate buffer pH 3 (50:50 v/v), flow rate 1 ml/min with iso...

As variedades de cana-de-açúcar utilizadas para a produção de cachaça, são as mesmas utilizadas para a produção de etanol combustível, no entanto, é muito importante que se conheça muito bem as características físico-químicas e de desenvolvimento microbiano na fermentação, que possam inferir na produção e qualidade da bebida. A fermentação é um est...

Las micotoxinas son compuestos tóxicos creados por hongos que contaminan productos agrícolas y representan un riesgo significativo para la salud humana y animal. Entre ellas, la Aflatoxina B1 es la más peligrosa debido a su alta toxicidad y potencial cancerígeno, particularmente en el hígado. La contaminación por aflatoxina es un problema de seguri...

In vitro synthesized circular RNAs (circRNAs) have emerged as a promising drug modality for RNA therapeutics due to their improved stability and reduced immunogenicity. However, effective analysis and purification of circRNAs pose critical challenges arising from the insufficient separation of circRNAs and linear RNA byproducts. In this study, we s...

A reversed-phase high-performance liquid chromatography (RP/HPLC-UV) method was developed for the rapid and simultaneous analysis of ascorbic and oxalic acids in both industrial and freshly squeezed beverages. Due to the rapid degradation of ascorbic acid, its stability was also investigated. The analysis was performed using an Inertsil ODS-3 colum...

The degradation of zearalenone (ZEN) in the rumen of dairy cows is influenced by rumen pH, which is a key factor affecting this process. The aim of this study was to investigate the variation of ZEN in interaction with other mycotoxins at different ruminal pH environments (physiological (pH 6.5) and acidic (pH 5.5)) using an in vitro rumen model. R...

Given the natural origins of flavylium derivatives, their chemical modifications, and their large potential uses in food, medicine, or green chemistry, the present review is a comprehensive study of flavylium-derived compounds. Several topics such as the green extraction and isolation techniques of flavylium derivatives including their chemical mod...

Este protocolo é adequado para a análise de compostos bioativos como cafeína, ácido clorogênico, mangiferina e outros em matrizes como extratos vegetais. O método utiliza HPLC acoplado a um detector de arranjo de diodos (DAD). English: This protocol is suitable for the analysis of bioactive compounds such as caffeine, chlorogenic acid, mangiferin,...

A lateral flow immunoassay (LFIA) for the detection of the banned fungicide carbendazim (CBZ) in food products was developed using rabbit-purified polyclonal antibodies. The strips were optimized and tested for sensitivity and selectivity with a cutoff of 0.20 µg/kg, which is considerably lower than the maximum residue level established for food. T...

Este protocolo é adequado para a análise de compostos bioativos como ácido clorogênico, mangiferina e cafeína em matrizes como extratos vegetais. O método utiliza HPLC acoplado a um detector de arranjo de diodos (DAD).

Group 4 metallocenes are competent catalysts for the oligomerization of higher α-olefins. Among the many chemical and physical variables of importance in the process, one is the choice of cocatalyst (activator). The impact of various activators on the performance of a representative catalyst, (nBuCp)2ZrCl2, in the oligomerization of 1-octene was th...

A comparative study on effect of chemical fertilizer and biofertiliser on Plumbago zeylanica for growth, yield and bioactive component was conducted at Bardoli (district-Surat), India between 2012 and 2013 using Random Block Design method and monthly observation of growth parameters. Application of biofertiliser Azotobacter, Azospirillum, Phosphate...

The Bourbon coffee cultivar has a high potential for producing a quality beverage, which is highly valued in specialty coffee markets. The objective of this study was to verify the occurrence of more promising genotypes for the production of specialty coffees in three different environments. In addition, the chemical composition of the grains was s...

Synthetic peptides are commonly obtained by means of solid-phase peptide synthesis (SPPS), in which separation of the peptide from the solid support requires treatment with 92.5% v/v trifluoroacetic acid (TFA); therefore, peptides are obtained as trifluoroacetate salts. For promising anticancer/antibacterial peptides it is essential to exchange the...

The purpose of this research was to investigate the optimization of the extraction process of total flavonoids from Davidia involucrata leaves and its bioactivity activity. The ultrasonic-microwave synergistic extraction (UMSE) method is used to extract total flavonoids from D. involucrata leaves. The effects of extraction time, extraction temperat...

A method of reverse phase high performance liquid chromatography (RP-HPLC) was established to analyze the dissipation of propamidine residue in tomato. Residue of propamidine was extracted from tomato using methanol buffered and determined by RP-HPLC with UV detection at 262 nm. The results showed that the average recoveries of the samples fortifie...

Polyethylene terephthalate (PET) plastic is one of the most widely used plastic primarily due to its flexibility, endurance, and low cost. However, the plastics one-time use nature and long degradation time have led to massive waste accumulation, damaging our ecosystem, health, and biodiversity. While previous degradation methods are ineffective du...

Total and soluble oxalic acids were extracted and analysed by HPLC chromatography following Asian cooking methods, which involved soaking, boiling and stir frying of silver beet (Beta vulgaris var. cicla) leaves. Autumn-grown silver beet leaves contained 1658 ± 114 mg/100 g dry matter (DM) of total oxalates, 954 ± 49 mg/100 g DM of soluble oxalates...

Ascorbic acid is a commonly-used food additive permitted in prepacked fresh meat preparations. Consequently, the addition of this antioxidant is subject to a specific packaging authorization. In this survey 180 samples of not prepacked fresh meat preparations have been analyzed in order to evaluate the diffusion of not authorized ascorbic acid addi...

Microwave-assisted extraction (MAE) followed by high performance liquid chromatography (HPLC) with a fluorescent detector (DAD) was used and developed for the quantitative analysis of the mycotoxin ochratoxin A (OTA) in commercial roasted coffee beans. This alternative approach has been compared with the conventional extraction that uses hydrogen c...

The widespread contamination of food by mycotoxins may present a serious hazard to human and animal health. The gamma rays were applied to reduce ochratoxin A (OTA) and aflatoxins B1, B2, G1 and G2 (AFB1, AFB2, AFG1 and AFG2) in almonds artificially contaminated. In the present study we investigated the effect of gamma irradiation dosages, ranging...

Vacciniumcyanococcus, most commonly known as blueberry, is a fruit native to North America that is known for its unique taste and high antioxidant content. The skin, seed and juice of both organically and conventionally grown blueberry extract were analyzed for antioxidant content using both the Hydrophilic and Lipophilic Oxygen Radical Absorbance...

Two analytical methods, reverse phase high-performance liquid chromatography (RP-HPLC) and formol titration, were compared for their accuracy and precision for the determination of a-amino nitrogen (a-AN) concentration in fish protein hydrolysates. The result showed that, when tested with pure amino acid solutions, RP-HPLC method had a better stand...

Total phenolic content, phenolic composition, and total antioxidant capacity of neck and bottom parts of two strawberry cultivars, ‘Chambly’ and ‘LL0220-10’, were evaluated using the Folin-Ciocalteu method, high performance liquid chromatography, and ferric reducing/antioxidant power, respectively. A significant interaction (p < 0.05) was observed...

The goal of the research was to use BBD, a productive RSM approach, to enhance the HPLC separation and validation of astilbin in Lysiphyllum strychnifolium stems. The percentage of acetonitrile (ACN), flow rate, and temperature were among the independent parameters that determined how much the chromatographic condition chosen from factor-level scre...

The bivariate calibration method was used for quantification of hydrochlorothiazide/enalapril maleate and hydrochlorothiazide/bisoprolol fumarate in pharmaceutical tablets. The determination of hydrochlorothiazide/enalapril maleate is made using new absorption wavelengths at 211 and 272 nm, while hydrochlorothiazide/bisoprolol fumarate is made at n...

El café (Coffea arabica L.) es una bebida popular, pero el café peruano enfrenta desafíos que afectan su competitividad en el mercado internacional. Se evaluaron las características fisicoquímicas, cromatográficas y la actividad antioxidante de dos tipos de café verde: el café caracolillo C y el café verde natural N. El café N presenta granos ovala...

Quality Control (QC) laboratories are constantly facing increasing pressure to improve efficiency and accuracy in the face of growing sample complexity and stringent regulatory requirements. This event aims to address these challenges head-on by providing insights into the latest industry trends and innovative solutions designed to empower QC labor...

Bitkilerin seconder metabolit bileşik profilinin tanımlanması ve miktarlarının belirlenmesi, gıda kalitesi ve özgünlük değerlendirmesi için önemli bir araçtır. Bu bağlamda, bu çalışmada, dört bitkinin seconder metabolit bileşiklerinin belirlemesi amaçlanmıştır. Anacardiaceae Rhus coriaria L., Tilia tomentosa Moench., Rheum ribes L., Rubus caesius L...

β-thalassemia poses a major public health problem in India. Reverse hybridization StripAssay® method is reported to be rapid, simple, reproducible and less expensive. The aim of the study was to detect occurrence of β-thalassemia carriers among the ante-natal mothers by Hb HPLC followed by detection of β-thalassemia mutations in antenatal mothers w...

In this study extracts obtained from fruits of bluebery and cranberry and rose leaves were chromatografied by using mobile phase variation and DAD detector. The chromatograms were evaluated under different mobile phase systems such as isocratic and gradient elutions following purification procedure. The best separation conditions of pigments of ant...

Polyurethane materials, widely used in indoor environments, occasionally exhibit unpleasant odors. An important source of polyurethane odorants is polyether polyols. Previous studies identified odorous 2-ethyldimethyl-1,3,6-trioxocanes in polyurethane materials and polyols but did not investigate the odor activity of the individual isomers. In the...

Background Aflatoxin B1 (AFB1), a potent carcinogen produced by Aspergillus species, is a prevalent contaminant in oil crops, with prolonged exposure associated with liver damage. Home-made peanut oil (HMPO) produced by small workshops in Guangzhou is heavily contaminated with AFB1. Despite the enactment of the Small Food Workshops Management Regul...

Evidence shows that vitamin D (Vit D) insufficiency is associated with some hormonal disorders. Hirsutism is a prevalent hormonal condition, which is usually associated with excess of androgenic hormones. Disturbance of Vit D level affects the regulation of these hormones and may be associated with hirsutism. This study compared the Vit D levels of...

SOP for how to handle of operation of Waters Alliance HPLC System.

This investigation examines the phenolic profile of Rumex acetosella L. harvested from two disparate locales within Iğdır Province, Türkiye, aimed at evaluating the impact of different locations on phenolic compounds. Employing High-Performance Liquid Chromatography (HPLC), a total of 19 phenolic constituents, including chlorogenic acid, caffeic ac...

The impact of light on beer degradation is a well-established phenomenon, leading to development of undesirable flavours or, in severe cases, complete spoilage. This study investigates the extent to which photodegradation is perceptible to beer consumers. Beer samples in commercial bottles were subjected to controlled light exposure to induce defin...

Farklı bölgelerde yetişmiş, sumak örneklerinden yağlanıp ve yağlanmadan bekletilen numunelerdeki, suda çözünen vitaminler HPLC ile tayin edildi. Bu amaçla, öğütülen sumak örnekleri iki kısma ayrıldı, bir kısmı yağlanırken diğer ikinci kısım ise yağlanmadı ve her iki grup altı ay bekletildikten sonra analiz edildi. Yağlanmamış sumak örneklerindeki a...

This study aims to detect ivermectin (IVM) residues in the liver and kidneys of sheep by using high-performance liquid chromatography (HPLC) technology. 50 samples (each of 25 livers and 25 kidneys) were collected from five different areas in Karbala Governorate. From October 2023 to January 2024. The analysis showed that samples of both liver and...

In this paper four branded black pepper, denoted as B1, B2, B3 and B4 were experimented using near-infrared (NIR) spectroscopy. NIR spectra were analyzed with multivariate analysis for discrimination and prediction based on piperine concentration (in ppm) measured using reverse phase high performance liquid chromatography (RP-HPLC) as reference val...

Understanding how climate change impacts berry ripening physiology is essential for selecting genotypes that balance sugars and acids under warming conditions. In this context, we used a portable near-infrared spectrometer in the vineyard, to monitor sugar and acid evolution in individual berries from 10 grapevine varieties over two years. Spectra...

This study is a review of the antidiabetic potential of the Saffron plant in the treatment of Type 2 Diabetes and research methodologies. The experimental design of this project study aimed to obtain crocin from the saffron plant. Crocin is considered a potential natural drug agent thanks to its high antioxidant and antidiabetic properties. However...

Эпоксиды известны как важные интермедиаты биотрансформации ароматических и олефиновых веществ, каталитически расходующиеся в присутствии кислот и солей переходных металлов. Обнаружено и кинетически изучено поглощение кислорода в реакторе манометрической установки тройной системой эпоксид стирола – гидрохинон – пиридин (ЭС – ГХ – Py). Скорость окисл...

It has been known that gamma‐aminobutyric acid (GABA) is increased in germinating seeds such as those of wheat (Triticum aestivum L.). Techniques such as ninhydrin reaction method and high‐performance liquid chromatography are used to quantify GABA levels. However, these methods require multiple steps and make it difficult to analyze multiple sampl...

The research was carried out at the tissue culture Laboratory of the College of Education for Pure Sciences, University of Diyala with the aim of induction of callus from leaves of Stevia rebaudiana Bertcni and estimate its content of Stevoiside compound in callus derived from plant leaves. The results showed that the best induction of callus was o...

Riboflavin (RF) sensitized photooxidation of pyridoxine HCl (PD) in the pH range of 2.0–12.0 has been carried out under UV and visible irradiation in aerobic and anaerobic conditions. PD follows first-order kinetics in the absence and presence of RF for its photodegradation. The first-order rate constants (kobs) for the photodegradation of PD in th...

Non-satisfactory bread-making quality in wheat, a Tajik staple, hampers food security in Tajikistan and calls for plant breeding efforts. Here, methods were searched for to study grain protein composition, which is of use for Tajik plant breeding to improve bread-making quality. Size-exclusion high-performance liquid chromatography (SE-HPLC) was us...

This study investigated the in vitro callus formation ability of various explants obtained from the Salvia officinalis (common sage) and the production of rosmarinic acid in the resulting calli. Four types of explants, including young leaves (1-1.5 cm), nodes, and stem pieces, were cultured on MS medium containing 0.8 mg/l 2.4-D, 0.5 mg/l NAA, and...

La dipirona, también conocida como metamizol, se emplea de manera extensiva para tratar tanto el dolor agudo como el crónico. Es efectiva en situaciones de dolor postoperatorio, migrañas, cólicos renales y dolores oncológicos, además de actuar como antipirético en casos de fiebre persistente. Para su análisis y cuantificación, se utilizan diversas...

Insulin antibodies (INAs) are found in both types of diabetic patients and have low binding capacity and high affinity leading to hypoglycemia or hyperglycemia in type II patients taking insulin, which differs from the insulin antibodies found in type I that contain a high capacity of insulin binding and low affinity. This work aims to detect the l...

Silymarin in the leaves, roots, and seeds of Silybum marianum (milk thistle) were analyzed by reversed-phase high-performance liquid chromatography and an ultraviolet detector. The content of silymarin and its constituents (taxifolin, silydianin, and silybin) was compared using water and ethanol (EtOH) extracts. Silymarin was only detected in the s...

An unexpected course of the reaction of hexafluorocumyl alcohol dilithium derivative 2 with N‐(t‐butylsulfenyl)phthalimide (3) has been presented. The process proceeded under mild conditions and resulted in previously undescribed chiral spiro‐system‐ 3′,3′‐bis(trifluoromethyl)‐3H,3′H‐1,1′‐spirobis(isobenzofuran)‐3‐one (5) as the only product. A det...

A new pre-treatment and determinatio