High-Performance Liquid Chromatography - Science method

This research work provides information about the concentration level of fat soluble vitamin D3 in different types of dietary supplements marketed in Bangladesh. Selected twenty-five samples were taken for analysis because physicians are prescribing randomly and available in local market. Vitamin D3 concentration levels were quantified using HPLC w...

Advanced oxidation processes constitute a promising technology for the treatment of wastewater containing non-easily removable organic compounds. In this research, the Fenton oxidation process was offered as an effective method for the removal of antibiotic sulfamethoxazole (SMX) from aqueous solutions. The effects of initial antibiotic concentrati...

Problem to deal with: Human serum albumin (HSA) is used as an excipient in the formulation of recombinant human erythropoietin (rhEPO) produced at Molecular Immunology Center. One of the challenges of our company is the diversification of suppliers. In this context, the use of a plasma-derived excipient, supplied by a new manufacturer, implies a ma...

In this investigation, the valorization of dried bamboo shoots and finding novel microbe that helps to produce high-yield biovanillin to meet market demand was discussed. An agro-industrial waste product dried bamboo shoots resists natural deterioration and when it builds up, it pollutes the environment. Ferulic acid was extracted from dried bamboo...

A selective eco-friendly micellar HPLC method was developed for investigation of moxifloxacin and related compounds in the presence of its degradation products. Central composite design was used to optimize the experimental conditions. The proposed method is based on isocratic elution on a C18 column using 92.5% (v/v) biodegradable aqueous mobile p...

Comfrey ( Symphytum officinale L.) root is a commonly known rich source of allantoin. This compound is widely used in pharmacy and cosmetology, but mainly in a synthetic form. However, the properties of natural allantoin in products of biological origin may be different from those of synthetic allantoin, especially due to the presence of other bioa...

Abstract Background and Aim Maintenance haemodialysis patients have increased morbidity and mortality which is mainly driven by an elevated inflammation level due to the uraemic milieu. A major part of this increased inflammation level is the degree of oxidative stress which can be assessed by albumin redox state (ARS). Aim of this study was to eva...

Abstract Mutisia L. f. (Mutisiinae, Mutisieae, Mutisioideae, Asteraceae) is a South American genus that contains several medicinal species. Mutisia acuminata Ruiz & Pav. and M. orbignyana Wedd. are known sources of the 12-dihydroxy-5-methylcoumestan and 5-methylcoumarin-4-glucoside respectively. The 12-dihydroxy-5-methylcoumestan exhibited notable...

Zusammenfassung Hintergrund Erkrankungen der Atemwege gehören zu den am häufigsten auftretenden Krankheiten im Kindesalter. Damit eine inhalative Therapie erfolgreich ist, sind vernebelte Tröpfchen in einem bestimmten Größenbereich nötig, der für Kinder besonders klein (< 3 µm) sein muss. Aerodynamische Charakterisierungen der Tröpfchengröße, hier...

Abstract The objective of this paper was to develop and evaluate two semi-solid pharmaceutical forms containing 0.1% tacrolimus: cream (CRT01) and gel (GLT01). For the evaluation of physicochemical stability, at times 0, 30, 60 and 90 days, at 23°C and at 40°C, High Performance Liquid Chromatography coupled with a Diode Array Detector (HPLC-DAD) wa...

Objective: A phenylbutenoid extract (PE) was obtained from Zingiber cassumunar rhizomes. Phenylbutenoids; namely DMPBD, compound D, and compound D acetate, have been identified as major anti-inflammatory and analgesic constituents. This present study aimed to formulate a gel containing PE that could be used as an alternative ultrasound gel for acut...

The combination of iron limitation and microzooplankton grazing controls phytoplankton productivity and taxonomic composition in high‐nutrient low‐chlorophyll (HNLC) regions. While increased productivity and diatom contribution triggered by iron enrichment support this view, direct measurements of underpinning group‐specific growth and grazing rate...

Microbial fuel cell (MFC) has gained importance for its ability to convert waste into energy. Exoelectrogens are the essential component of MFC that plays an important role in determining the efficiency of MFC. Therefore, the current study aimed at isolating efficient exoelectrogen(s) with significant biodegradation ability. Based on iron reduction...

A nanocomposite material based on halloysite and α-lipoic acid was studied. The kinetics of the degradation process of α-lipoic acid under the influence of ultraviolet radiation and thermal stress in its native state and in the composition of a halloysite-based nanocomposite were studied. The concentration of undegraded α-lipoic acid and the effect...

Quality coffee consumption is increasing nowadays; however, quality can only be achieved through appropriate cultivation and processing techniques. The study is aimed at using high-performance liquid chromatography (HPLC) and spectrophotometric techniques for evaluating different coffee varieties grown in Ethiopia based on caffeine, antioxidant, an...

Tea is the most popular beverage consumed in the world after water. In the present study, evaluation of inher- ent sugars viz. sucrose, glucose, and fructose in fresh tea leaves and in black tea were quantified using High Performance Liquid Chromatography. Fructose, glucose, and sucrose concentrations in the third and fourth tea leaves were signifi...

RNA-based therapeutics and vaccines are opening up new avenues for modern medicine. To produce these useful RNA-based reagents, in vitro transcription (IVT) is an important reaction that primarily determines the yield and quality of the product. Therefore, IVT condition should be well optimized to achieve high yield and purity of transcribed RNAs....

This study used cyanamide as the precursor and SiO2 as the template to prepare a layered structure g-C3N4, and characterized its crystal structure, morphology, optical performance, and electrochemical performance. Photocatalytic oxidation experiments were conducted on biomass derivatives (glucose).Orthogonal experiments were used to study the effec...

Este estudo investigou os efeitos das condições operacionais do processo Fenton na degradação do herbicida atrazina em águas residuárias. Propôs-se um delineamento composto central rotacional (DCCR) 2², cobrindo 11 ensaios, com os seguintes fatores: a concentração de Fe2+ (2,95; 5,00; 10,00; 15,00; 17,05 mgL-1) e a concentração de H2O2 (29,50; 50,0...

Mycosporine-like amino acids (MAAs) are natural UV-absorbing compounds found in microalgae and macroalgae. The content of MAAs in algae varies with the seasons and environmental factors. Red alga dulse in Usujiri (Hokkaido, Japan) is an underutilized resource. Therefore, we investigated the amount of MAAs in Usujiri dulse in 2022 to clarify the sui...

The contents of indapamide and related impurities in generic indapamide sustained-release tablets were simultaneously detected by a single-run high performance liquid chromatography equipped with photodiode array detector (HPLC-PDA) method for the quality control in this paper. The results showed the method had a good selectivity and was validated...

This work uses resonant Raman spectroscopy (RRS) to investigate changes in carotenoid concentration in extra virgin olive oil (EVOO) as it oxidizes under accelerated thermal aging. Carotenoids are nutritious antioxidants and biomarkers that represent the general quality of olive oil. HPLC is the conventional method used to determine the concentrati...

This comprehensive white paper embarks on a journey through the landscape of high-performance liquid chromatography (HPLC) market trends in the United States, focusing on its critical role in advancing HbA1C and hemoglobinopathy testing at point-of-care (POC). HPLC, a versatile analytical technique, has emerged as a linchpin in accurate diagnostic...

Background and Objectives: Gluten-free products have been produced as part of medical therapy and have gained popularity among individuals seeking weight loss or healthier dietary options. Assessing the potential risks associated with these products is essential in optimizing their compositions and developing new dietetic approaches. This study aim...

Познаването на физикохимичните характеристики набиологично активните вещества може съществено да спомогне заопределянето на оптималните условия за приложението исъхранението им. Такива характеристики са технитедисоциационни константи (pKa, pKb) и липофилност.Дисоциационната константа на веществата, рКа (рКb) ипараметърът на липофилност (log P) са в...

Total polyphenol and total flavonoid assays were performed to characterize the relationships between the color of Peucedanum japonicum (PJ) seed coat and stem and the content of phytochemical compounds. The samples were divided into two groups based on their stem and seed coat color, with each group containing 23 samples. The stem color group was s...

The aim was to investigate the association between bisphenol A (BPA), 25-hydroxy vitamin D [25(OH)D], and 1α,25 dihydroxy vitamin D [1,25(OH)2D] levels in the cord blood of newborn babies. BPA was measured by high pressure liquid chromatography (HPLC) and vitamin D levels by commercial ELISA or ECLIA kits. BPA and Vitamin D levels were grouped acco...

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This study aimed to investigate Aflatoxin‐M 1 (AFM 1 ) contamination in pasteurized and raw milk samples consumed in Kerman and Rafsanjan in southeastern Iran. In this cross‐sectional study, a total of 100 samples of raw ( n = 67) and pasteurized ( n = 33) milk were randomly collected from retail stores, supermarkets, and milk transport tankers in...

The dissipation study of residues of insecticides having different modes of action was studied in cabbage. The insecticides such as deltamethrin, chlorantraniliprole and fipronil residues were sprayed as foliar application twice at an interval of 7 days with the recommended doses of deltamethrin (@ 12.5 g a.i./ha), chlorantraniliprole (@ 30 g a.i./...

Protocolo Abrangente para a Extração de Biomassa de Cianobactérias Cultivadas em Laboratório. É crucial prestar uma atenção meticulosa a materiais de qualidade, envolvendo solventes de grau HPLC, microtubos imaculados e ponteiras Eppendorf Quality nunca antes utilizados. Controles rigorosos de qualidade abrangem "Branco de Cultivo" (meio de cultivo...

Penelitian ini dilakukan untuk melakukan isolasi, pemurnian, serta karakterisasi etil-para-metoksisinamat (EPMS) dari rimpang kencur (Kaempferia galanga L.) sebagai bahan alternatif alami tabir surya. Isolasi dilakukan dengan metode maserasi 395,362 g kencur kering menggunakan etanol yang dilanjutkan dengan pemurnian melalui partisi dan kolom denga...

Glucosinolates (GSLs), compounds with potential chemo‐preventative properties, can be affected by pre‐ and post‐harvest processes. Therefore, monitoring their content is important for the food and agricultural industries. This study evaluates the application of mid‐infrared (MIR) spectroscopy for the determination of GSLs in commercial broccoli ( B...

Background A quick, low cost and precise method was developed and validated for flutamide in the rat plasma using a high-performance liquid chromatography. Acetanilide was used as internal standard in the study. Flutamide was extracted from rat plasma using methanol. The chromatographic analysis was carried out on Cromasil C18 column at 227 nm usin...

Green MAE (microwave extraction) process was used to extract natural flavors (vanillin) from Madagascar Vanilla planifolia beans. Experimentally, we used a factor to study the influence of parameters on extraction efficiency, and response surface methodology (RSM) was used to obtain interactions between the key influencing parameters. The present s...

Family 1 glycosyltransferases (GT1s, UGTs) form natural product glycosides with exquisite control over regio- and stereoselectivity, representing attractive biotechnological targets. However, regioselectivity cannot be predicted and large-scale activity assessment efforts of UGTs are commonly performed via mass spectrometry or indirect assays that...

Low temperature (LT) and mechanical wound (MW), as two common physics methods, have been empirically used in production to stimulate the primordia formation of Flammulina filiformis , which is typically produced using the industrial production mode. However, the detailed effect on the fruiting body formation and important endogenous hormones and si...

O trabalho em casa de vegetação foi realizado com o objetivo de verificar o acúmulo de ácido chiquímico em soja geneticamente modificada em diferentes estádios fisiológicos e partes da planta. O ensaio foi realizado no Centro Experimental Central do Instituto Biológico em Campinas, SP, em fitotron no delineamento inteiramente casualizado (DIC) com...

Various photoautotrophic cyanobacteria accumulate intracellular poly(3-hydroxybutyrate) (PHB) granules. This protocol can be used for determining the PHB contents of the cells as % PHB weight per dry cell weight using acid hydrolysis followed by high-performance liquid chromatography (HPLC). This HPLC analysis is rapid, with a running time of appro...

This study aimed to establish a method for the simultaneous determination of isoquercitrin, trifolin and afzelin in A. persica flowers by high performance liquid chromatography (HPLC) with ionic liquid as extractant and ultrasonic-assisted extraction. The effects of ionic liquid concentration, solid–liquid ratio, number of crushing mesh, ultrasonic...

There are numerous medicinal plants in the Southern and Eastern Nigeria. These plants are widely utilized in Nigerian traditional system of medicine for the treatment of countless of illnesses. This paper focused on the phytochemical and essential composition of the aerial parts of Leonurus cardiaca. The phytochemical and essential oil screening an...

In this study, the residue levels of chiral pesticide hexaconazole during kiwifruit juice processing (peeling, homogenization, and sterilization) were investigated by using high-performance liquid chromatography (HPLC), and the dietary risk during these processes was also assessed. Hexaconazole was applied at dosages of 173.33 and 346.66 mg/L (reco...

In this study, the efficiency of two detectors—variable wavelength detector (VWD) and fluorescence detector (FLD)—in a liquid chromatograph to quantify azoxystrobin in water was compared. For this, a method was developed in a HPLC-VWD-FLD system using extraction by modified QuEChERS. The detection of azoxystrobin by fluorescence was possible using...

Acrylamide is a neuro-and reproductive toxicant to humans and has carcinogenic efects in animal species which naturally forms in high-carbohydrate foods at high (≤121°C) temperatures. Tis study assessed acrylamide formation in various Iraqi bread types, including whole-wheat fatbread, white fatbread, stone-baked bread, whole-wheat baguette, and whi...

Ciprofol (HSK 3486, C14H20O), a novel 2,6-disubstituted phenol derivative similar to propofol, is a new type of intravenous general anaesthetic. We found that the exhaled ciprofol concentration could be measured online by ultraviolet time-of-flight mass spectrometry (UV-TOFMS), which could be used to predict the plasma concentration and anaesthetic...

The purpose of this study was to investigate the differences between the phenolics compositions of bilberry and blueberry liqueurs, to determine whether these are comparable. Both bilberry and blueberry liqueurs have the same name on the market, however they differ in phenolic composition and content. A total of 43 compounds were identified, 36 in...

The objective of the present study was to investigate the residue depletion of EF and CF in yellow catfish to estimate its WTs in plasma and tissues after multiple oral doses for 3 days at 20 mg/kg at 15, 20, and 25 °C. Samples were collected at pre-designed time points after oral doses. A validated method was performed to quantify EF and CF in pla...

Objective: Preeclampsia is a multifactorial disease characterized by high blood pressure and protein in the urine. In this study, we investigated the association of vitamin D binding protein (GC) and vitamin D receptor (VDR) gene polymorphism with the risk of developing preeclampsia. Methods: 25-hydroxyvitamin D was measured using High-performan...

Fluorescent probe technology was discovered in the 19th century and imaging techniques were applied to microscopy around 1670. Fluorescence microscopic imaging is a classical method for observing the structure of organisms in the life sciences. In this regard, single-molecule fluorescence imaging uses fluorescent probes to label, detect, and analyz...

Rationale Isolation of underivatized amino acids (AAs) using high‐performance liquid chromatography (HPLC) is becoming a popular method for carbon (δ ¹³ C) and nitrogen isotope (δ ¹⁵ N) analyses of AAs because of the high analytical precision and for performing dual‐isotope analysis. However, some AAs in natural samples, especially small, hydrophil...

High performance liquid chromatography (HPLC) pump has a main function to deliver a mobile phase which need accuracy and stable flow rate. Recently, three flow rate calibration methods are used to test the HPLC pump performance such as using standard flow meter, gravimetric method, and volumetric method. These calibration methods have not been clea...

Acid Red 73 (CI 27290) is a prohibited component in cosmetics, particularly in hair color formulations. The purpose of this investigation is to discover the coloring additive Acid Red 73 in cosmetic hair dye formulations. Acid Red 73 (CI 27290) is a sulfonated azo dye that is manufactured to be more hazardous than other colors and is damaging to th...

This research study aims to evaluate the effect of ultrasound (US) pretreatment on the quality of crude palm (Elaeis guineensis) oil (ACP) during three stages of the extraction process: 1) press liquor (LP), pre-clarifier input (EC), and pre-clarifier output sludge (LS). Spectrophotometry is used to determine the bleaching deterioration index (DOBI...

An increased incidence of colorectal cancer (CRC) has been observed among cholecystectomized patients. However, the exact mechanism is not fully understood. Therefore, this study aimed to compare the effects of hepatic and gallbladder bile on normal and cancerous colon cells and to separately examine the effects of the most common bile acids (BAs)....

Compounds of p-hidroksibenzoic and p-coumaric acids are allelochemicals produced by plants and can be phytotoxic to other plants. Allelochemicals can be used as bioherbicides in weed control. The objective of this research was to determine the content of p-hydroxybenzoic and p-coumaric in rice roots, and their effect on the growth of barnyard grass...

We elaborate the chemical synthesis of polyprenols by chain lengthening, which is considerably less time-consuming than the other previously described methods. Our method eliminates critical steps requiring low temperature and toxic chemicals, which are difficult to perform in ordinary laboratories. The critical step of acetylene addition in liquid...

Pseudothiohydantoin derivatives have a wide range of biological activities and are widely used in the development of new pharmaceuticals. Lipophilicity is a basic, but very important parameter in the design of potential drugs, as it determines solubility in lipids, nonpolar solvents, and makes it possible to predict the ADME profile. The aim of thi...

Insecticides are substances or chemicals that are used to kill or eradicate insects. One of the active ingredients contained in insecticide products is permethrin. One of the tests carried out was testing the levels of the active ingredient such as permethrin in insecticides. This testing was carried out by High Performance Liquid Chromatography (H...

A simple, rapid, accurate and precise RP-HPLC method was developed and validated for the determination of ceftrioxone sodium in parentral dosage form. Chromatographic analysis of the drug was achieved on CYBER LAB HPLC comprising of LC- 100 P pump, a variable wavelength programmable LC-UV100 UV detector and SCL system controller. Flowrosil C18 colu...

The present study was to develop and validate a rapid analytical method of high-performance liquid chromatography with UV (HPLC/UV) detection for the determination of levetiracetam (LEV) concentration in human serum and cerebrospinal fluid (CSF) samples. The newly developed method was used to measure LEV concentration in the serum/CSF of 29 patient...

In insect ionotropic γ-aminobutyric acid receptor (iGABAR) subunits, only resistance to dieldrin (RDL) can be individually and functionally expressed in vitro. In lepidopteran, two to three RDL subtypes are identified; however, their physiological roles have not been distinguished in vivo. In this study, SlRdl1 and SlRdl2 of S. litura were individu...

The analytical method development in Pharmaceuticals is most important because it is the regulatory requirements. The determination of Active Ingredients of Pharmaceuticals (API) and Excipients is only possible when the testing method will be validated as per ICH, WHO guidelines or GMP requirements. The development will be reliable when the correct...

Resumen Objetivos La cromatografía líquida de alta resolución (HPLC) es una de las técnicas empleadas para determinar la hemoglobina glicosilada (HbA 1c ) y el método de elección para el cribado de hemoglobinopatías estructurales. El objetivo de este caso es mostrar cómo en un análisis de rutina de HbA 1c es posible encontrar incidentalmente una h...

Atrazine, the most commonly used herbicide, has been reported to pollute the water environment and do harm to human health. It is thus urgent to find an efficient way to degrade atrazine. Although various advanced oxidation processes including high voltage electrical discharge (HVED) have been applied to degrade atrazine, the formation kinetics of...

Introducción: Mitomicina-C está aprobada por la Agencia Española de Medicamentos y Productos Sanitarios (AEMPS) para administración intravenosa e intravesical. La Mitomicina-C puede administrarse diluida en glucosa 20% tal y como aparece en la ficha técnica. El objetivo de este estudio es determinar la estabilidad de la Mitomicina-C en glucosa 5% y...

Background: Storage of samples may be necessary prior to testing drug levels in certain study designs; however, the effect of storage duration on measured drug levels is not known for all drugs. Objectives: The objective of this study was to evaluate the stability of carprofen in canine plasma when stored at -80°C for 6 months. Methods: Six he...

The impact of hydrogen infusion into solvents on the extraction of phytochemicals was evaluated. Pure (water, ethanol, methanol) and hydrogen-rich solvents at temperatures (25°C and 35°C) were used for extracting phenolics, flavonoids, anthocyanins, and antioxidants from lemon peel. Incorporating hydrogen into all solvents increased the extraction...

Introduction Currently, there is an active development of methods for the laboratory diagnosis of alcohol abuse using biochemical markers. Objectives The aim of this work was to estimate the prevalence of alcohol abuse among the urban population of Belarus using the concentration of phosphatidylethanol in the blood as a biochemical marker of alcoh...

Oligomers are a particular category of non-intentionally added substances (NIAS) that may be present in food contact materials (FCMs), such as polyethylene terephthalate (PET), and consequently migrate into foods. Here, an ultra-high-pressure liquid chromatography quadruple time-of-flight mass spectrometry (UHPLC-qTOF-MS) method was developed for t...

In a previous study, we demonstrated that the carotenoid neoxanthin was contained in young leafy vegetables including spinach and showed a fat accumulation inhibitory effect in vitro. To evaluate the bioavailability of neoxanthin, a raw young spinach leaf (100 g day-1 for 4 weeks) intake test was performed on 14 participants (36.5 ± 8.0 years; male...