Questions related to Hepatoprotective
I am currently working on the hepatoprotective effect of pomegranate by CCl4-induced liver injury in C57 mice. This is my final year research project for my BS(hon).
I am working on the hepatoprotective effect of some medicinal plants and, i want to use the ccl4 induced acute liver injury ( 7days protocole)
there are a plenty of articles that treat the subject, and each one use differente concentration !!
so according to your experience what is the best and most used ccl4 concentration to induce mice acute liver injury ??
ALT is a biomarker of liver damage, generally used to investigate hepatoprotective activities. Usually this marker is quantified in plasma/serum, but sometimes, it can also been assessed in liver homogenates.
1- May you share with me some publications reporting ALT assay in liver homogenates, After a chemical injury ?
2- using apap as an example of hepatotoxicant, Do you think ALT values in liver should increase or decrease. Because I saw some conflicting publications on that.
Thanks in advance for your suggestions.
I have done in vitro hepatoprotective activity of diff. conc. of my plant extract in HePG2 cell lines using Paracetamol as toxicity inducer and silymarin as reference drug. I am in confusion to use which method to use for statistical analysis of data. Whether two way ANNOVA, one way ANNOVA need to be used or independent t-test is sufficient for analysis? Please guide me regarding this.
I am working on hepatoprotective activity. As a part of my study I have to conduct histopathological study of larvae. I went through different research papers. But each paper showing different procedure. Can any one share exact protocol for fixing zebrafish larvae by using 4 % PFA.
Recently I have done with my hepatoprotective experiment where I used CCl4 to induce liver damage. While evaluating my hepatic marker, unfortunately I have got higher serum AST level in the reference control (222 U/L) than the hepatic control (156 U/L) group. The other marker is ok. How can I validate the data with my statics? Badly need some suggestions what to do?
im working on the antioxidant and hepatoprotective potential of Azolla microphylla . please help me with the simplest method to synthesis the gold nanopareticles from azolla.
In liver hepatoprotective studies, we use many animals and we incur high financial costs. Can we use ovo in the experiments of evaluating the efficacy of plant extracts in protecting the liver by injecting the extract into fertilized eggs and observing their effect on the fetus?
Seanol (Dieckol) has been reported to show various biological activities, such as anti-inflammatory, antioxidative, hepatoprotective, antidiabetic, anti-allergic, antiviral and anticancer activities. Moreover, it is intermediated agent which well disperses in polar solvents. How to conjugate Seanol (Dieckol) with the surface of porous silica nanoparticle or modified silica particle for biological applications ?
I have tested the hepatoprotective effect of a natural compound in cyclophosphamide-induced rats and I want to test it in vitro using HepG2 cell line. Because my main target is oxidative stress, I want to induce oxidative stress in HepG2 cells using cyclophosphamide. I found other models like CCl4, cyclosporine and hydrogen peroxide induced oxidative stress in HepG2, but I didn't found any about cyclophosphamide. Do you have any idea?
I am investigating the hepatoprotective effect of some medicinal plant on chronic carbon tetrachloride induced liver injury. I observed that although some of the medicinal plants increased the antioxidant enzyme (SOD, CAT and GSH) level in rats co-exposed to CCl4 and the extracts while they also increased lipid peroxidation and markers of hepatic injury such as ALT and AST.Has anyone experienced something similar? If yes, what are the likely reasons why this happened?
We are evaluating the hepatoprotective effect of a plant extract against a chemical carcinogen. Following treatment of the induced rats with the plant extract, we observed increased serum albumin levels when compared with the control group. Interestingly, we found a significant increase in serum albumin compared to the NORMAL group. Is there any explanation?!
I have to carry out hepatoprotective screening of few ethnomedicinal plants using alcohol as hepatotoxicant. I went through several papers, but none has mentioned the alcohol percentage orally to be given to rats. Kindly help
What is the role of hepatoprotective products in treatment/management of hepatitis B virus infection?