Questions related to Hematology
I have used Mindray BC-3000plus Hematology analyzer for research purpose.
Some information about may machine, Mindray BC 3000 Plus Fully Automatic Hematology Analyzer - 3 Part. Operation Mode-Fully Automatic, Brand-Mindray, differential Type-3-Part, Number of Parameters-19, Chamber Type-Double Chamber, Instrument Type-3 Part Hematology Analyzer. of running samples it displays error message gives error (background abnormal , WBC clog &RBC clog, when it aspirate sample or blank there red dots under screen but WBC and RBC remain zero it doesn't up count .finally it display preparing count time similarly WBC and RBC remain zero, empty histogram displayed) I was checked all setups , Maintained stepwise based on manual instruction ,cleaned all valves ,change WBC bath and I checked all setting I found that WBC reference count time and absolute WBC count time has 4 seconds differences up software all is done. I hope I may get solution from you for my problem faced?
A strong Association has been described between Blood group O and Peptic ulcer, Blood group AB and Carcinoma cervix, Blood group A and Gastric Carcinoma , Blood group B and Pemphigus and Seborrhoric Dermatitis.
Here is what the lab said when I sent out my samples to have CBC ran, the first time I sent out they said samples were clotted. I thought it must be due to not inverting sample EDTA microtainer enough. Also I switched from saphenous vein to the tail vein for the second collection. All samples were taken beautifully and I had at least 400ul of whoole blood in each tube. The samples sat at room temperature then packaged in a cooler with fridge packs not touching samples in box. Next day here are my results:
Hematology samples received this morning. Some samples were grainy. See attached picture. We made slides on for them but some just look unusable. Some of the slides did not contain a mono layer to where we can make an appropriate differential (distinguish between the different types of white blood cells). RBC’s and WBC’s were disintegrated and hard to tell what they are.
We tried running a few of the samples and the values does not look valid either. The hgb (hemoglobin) is the same value as the hematocrit.
Can ANYBODY help me?? Explain why this would happen, mu boss isn't very happy right now...
ANy help would be so much appreciated.
Can anyone share a protocol for a ferrozine assay for tissue samples that is reproducible, including the lysis protocol? Also, what is the minimal amount of iron detectable using this protocol and which method was used to quantify the tissue amount (by protein?)?
We are reaching you out because we are working on a project at the RWTH university Aachen, called Epi-Blood, which enables counting of leukocytes in frozen blood retrospectively, with high accuracy and requires only low volumes. www.Epi-Blood.de
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I work in a cancer lab and have been trying to isolate and quantify platelets without the use of any advanced hematological instruments. I don't need pure platelets, so I've just been generating platelet-rich plasma and trying to get a rough count using a neubauer chamber. With this method though it's incredibly difficult to figure out what I'm looking at under the microscope, and counting platelets has been impossible. Does anyone have experience with this?
Current PRP isolation protocol:
1. Collect blood from mice after CO2 euthanasia via the posterior vena cava, collect into sodium citrate (1:9)
2. Centrifuge whole blood @300g for 20min
3. Collect upper 2/3 plasma fraction
4. Centrifuge fraction @800g for 15min
5. Resuspend in PIPES buffer, pH 7.4
6. Dilute 1:20 and add 10uL to Neubauer chamber
7. Allow cells to settle in moist environment for 15min, try to count
I have a question regarding the hematology analyzer for complete blood count for blood samples. I would need to analyze fixed blood samples and I would like to know if the analyzer could work also with this kind of samples. I know that the analyzer detects electric properties of the cells and with fixed samples I guess it won't be possible. Does anybody have experience with that? Thank you for your help!
The website addressed to the above link goes inaccessible at times. The reasons are unknown. This is a key database accessed by many hematopoiesis researchers. if anyone knows alternative ways to access the database, kindly post here, which will be useful for many researchers. Thank you.
The use of the RefVal freeware for establishment of blood reference intervals for wildlife species has been popular in recent years, however the add-in doesn't appear to be compatible with Excel in Office 365. Has anyone else got around this problem, or if not what are you using that's equivalent? MedCalc is good, but expensive. Thanks!
I am currently doing an experiment which involves changing the techniques in staining of blood smear. I use expired EDTA tubes to store the blood collected, does the expired tube will affect the morphology of blood in any way when I view it under the light microscope?
There has been evidence that those with minor imbalance of blood cells [for unspecified condition], in which the red blood cells are small and less in quantity than white blood cells, can be of benefit by a dosage of Vitamin C and daily moderate exercise.
When in the case of shortness of breath, while at the same time having such small in size red blood cells, were able to show benefit by a dosage or iron and Vitamin C in a single dose. (this was not a contraindication of the particular case)
Can you verify that cases of unspecified shortness of breath that may be correlated to small-sized red blood cells, can benefit from a monitored vitamin dosage?
Similarly, Co-Q10 shows benefit for those who may have heart damage. Of course, a doctor must manage patient supplements.
We have recently seen a case of large diffuse B-cell lymphoma of probable folicular origin (CD10+ partial expressión) with a well defined CD103 expression by B-cells. The references in the literature are scanty and unclear.
I am trying to compare fibrinolysis between two genotypes of mice after an insult. The D-Dimer levels were equal between the mice but I was told that rapid fibrinolysis (which we're expecting) would form fibrin degradation products instead of D-dimers.
I've found a few ELISAs of unknown quality but I'd really like an antibody I can use for western blot. Preferably I'd like one that can detect multiple fragments so I can see if production of one fragment is different over the others. One of the main issues so far is that there are very few hematology reagents for mice. Most are for humans.
i need this information for preparing fish feed to analyze the effects of nano particles on fish growth and hematology.
I am doing a master in nursing but still does not have any idea about what topic I am going to choose. pls help
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I was posed a question by a hematopathologist in training this morning: Why are B-cell lymphomas more prevalent than T-cell Lymphomas?
My initial guess was perhaps there is increased tolerance training in T-cell populations, but it turned out she did not know either. Her best guess was that it is multifactorial in nature, i.e. genetics, viral infections (HTLV), and environmental factors.
Does anyone have any papers or book chapters for reference? I would love to be able to bring a more detailed and concise answer back to her. So far, I haven't found anything that answers the question on PubMed.
Thanks in advance.
Hello, I am an undergraduate student . Currently I'm working on "Hematological diagnosis using blood sample images " project . I am working on developing a deep learning model for this diagnosis . Now I need useful research materials to continue my research . It will be really helpful if free research papers were provided regarding this topic.
I'm trying to set up a protocol for PBMC isolation from whole blood using density gradient centrifugation. In a previous lab we diluted the blood in RPMI prior to layering, and washed the PBMCs in RPMI, but a lot of protocols I've seen use PBS instead. Is there any reason to use one or the other, or do they both perform the same function?
Haemoglobin is a serious problem during purification and analysis of antioxidant enzymes from red blood cells. For this reason it could be necessary to remove Hb in order to obtain a clear supernatant.
It's been a while, while some patients in whom high grade fever, prolonged duration of fever doesn't subside and no early diagnostic tests and hematological examination turn out to be positive, fungal infection has been seen either in urine or even in blood culture in few patients. Non albican type fungal infection seems to be one of the important cause of high grade fever in spinal injured patients.
Hi. Good day. I am currently working on ROC Curves to determine the *specificity, specificity, negative & positive predictives value and the likelihood ratio of pos & negative values. I am looking for cut off points in IDA of its hematological markers (FBC, FBP, RDW, MCH and MCV). I would like to ask, if i manage to plot the curve using SPSS, it will automatically provide me with *these values or do i need to do crosstabs or other test to find these. Manually there is a formula to calculate these, but in spss? and how to discuss the output. Thanks
Biological techniques are methods or procedures that are used to study living things. They include experimental and computational methods, approaches, protocols and tools for biological research.
Is bcl2+ in DLBCL always associated with poor outcome in PFS or OS, or is this finding significant only in high IPI score DLBCL?
Imatinib is a tyrosine kinase inhibitor and used as cancer drug for some hematological cancers.
Any synthetic analogue of Hemoglobin or any other Xenobiological agents can be cited as example. Please provide links and references.
I'm using commercially available Drabkin's reagent to measure the hemoglobin content of blood photometrically. The reagent I bought contains sodium bicarbonate, potassium ferricyanide and potassium cyanide. In my understanding, all different forms of hemoglobin (except sulfhemoglobin) are converted to methhemoglobin by potassium ferricyanide in a first step which reacts then with potassium cyanide to cyanomethemoglobin which shows a significant absorbance at 540 nm.
But sometimes I see a second peak around 575 nm (indicated by the red arrow in the graphic), sometimes very weak but sometimes also very strong and I wonder what it could be. Could this be some remainings due to uncomplete reaction or could it maybe be due to poor quality of the sample?
Thank you for your advice!
How would you score Daflon (Diosmin and Hesperidin) with regard to blood thinning effect? How would you rate it in comparison with Aspirin, Warfarin, other agents? I.E do you consider it to be potent? Mild, moderate?
I stained mouse peripheral blood with B220 and CD3 markers and found all the CD3 postivie cells were B220 positive too. My aim was to distinguish simply B cell and T cell by these markers but I am confused now. Any comments, or advice?
I plan to conduct a study in which I will compare depression and quality of life among children with cancer to children with other chronic conditions (hematologic, kidney and respiratory chronic conditions). Is it appropriate to say that the cancer patients are case and other children are control. Or should I change the study design?
In case my case group are children with cancer, who should be the control group?
I need to differentiate HSCs towards granulocytic lineage, though I have no access to IL-3. I was wondering if I can do the above only using GCSF, FLT3, and SCF.
I appreciate your consideration in advance.
My Dad (66 years old) has recently beed recently diagnosed with multiple myeloma. Apart from significantly elevated monoclonal protein, he has no symptoms. He has had his blood protein levels high for the past 5 years. Detailed medical exams showed that his bones, kidneys, and blood work (other than what I have already mentioned) are all fine. My Dad has always been fit and he is still feeling well overall.
He has an opportunity to join a clinical study that uses daratumumab combined with standard treatment for multiple myeloma. My understanding is that the treatment he would get is considered chemotherapy.
Is undergoing the chemotherapy the right thing to do now, considering multiple side effects of it (with or without daratumumab)? Or should he wait until symptoms begin to appear. My Dad’s doctors do not seem to share the same opinion on this matter.
I would deeply appreciate suggestions. Please note that I am a neurolignuist, not an MD. Therefore, I may have worded something incorrectly here. However, I will gladly clarify or provide more detailed information if helpful.
Thank you very much in advance,
I need research and literatures for my MSc Thesis, effect of petroleum refinery effluent on albino rats using moringa plant or seed extract as abatement. There are few research and study conducted so far on this that i have found. Could be that the effluent are completely free from pollutants which can be toxicant to human. Could we suggest that the industrial waste water are completely processed and treated before discharge to the environment?
This is a challenging case I've been facing, about a 65-year-old lady presenting with weekly recurrences of herpes infection on sacrum and inner thigh (most likely HSV type 2) in the last 20 years. Recurrent vaginal candidiasis coexists. I suspected of acquired immunosuppression and her immunological panel revealed:
Complement levels and other immunoglobulins are within the normal range.
She takes the following drugs continuously (could any of them explain leukopenia + lymphopenia?): omeprazole, ranitidine, amitriptyline, desvenlafaxine, pregabalin, and vaginal ovules of boric acid.
I prescribed imiquimod cream locally, 3 times a week, and she reports parcial improvement of frequency and severity of skin infection.
My doubts now are regarding the continuation of propaedeutics and therapeutics. Any thoughts? Relevant literature?
I am studying about reference interval of hematology parameter followed CLSI guideline. Then i got some difficulties in percentage of eosinophil. I got my study subject have >0 value. But in Robust method i got minus lower limit? How it be possible? Anyone can help me to raise my lower limit?
Hello, could anyone share your experience regarding CBC analysis in sinovial fluid samples and pretreatment with hyaluronidase? Is it absolutely necessary for every sample to be treated with the enzyme? Our equipment include Siemens Advia 2120i hematology analyzer.
i m working on hematology of silver carp(Hypophthalmichthys molitrix).i want to know the value of hemoglobin, RBC count, WBC count MCV, PCV, MCHC, neutrophils, eosinophils, lymphocytes and monocyted of a healthy silver carp.kindly guide me?
Dear all friends,
I am a graduated PhD student of laboratory hematology and blood bank from Iran. I am so keen to continue my studies in Postdoc grade particularly in Germany. I have strong potentials to do research activities as work-team. My priorities are cellular biology and stem cell-related experiments. If anyone knows institutions that I can apply for a Postdoc position, please notify me. Also, if there is any necessity to send my CV for more information, please do not hesitate to tell me. Thanks in advance for your entrancement.
I want to make a stable blood sample. In my study, I want to make a comparative study of many hematology devices and I want to try many blood sample from different sources.
I've seen many protocols, all of them old Patents, I don't know which I should depend on.
Every hematology textbook, the chapter of idendtification of common Hb variants mention about electrohoresis in agar gel with citrate buffer at pH 6.
I have discussed with many eminent hematologists in India and found that nobody is using this method any more because of new technology available, but also none of them have first hand experience about the method. Why so ?
I need two detailed and reliable lab protocols one for assessing NK cytotoxicity and one for NK degranulation assay using flow cytometry.
Is NK cytotoxicity done only in co-culture with K562 or any other hematological cell line such as U937 can be used instead?
I would highly appreciate if anyone could help.
Thank you in advance!
This is the sevent exercise regarding my proposal to share our professional experiences in hematologic morphology. Most of previous exercises aroused interest and participation. Some, such as the sixth, have not piqued your interest. I propose the seventh case that I hope will generate interest and participation.
There are images from BM aspirate of a 79-year-old man with pan-cytopenia. Numerous cells have blastic features: increased size, exiguous cytoplasm, dispersed nuclear chromatin without evidence of nucleoli. Immature granulocytes and NRBC are present.
Hi there! I am wondering if there is any way of transiently increase platelet count in mice, like administrating TPO or something like that?. Thanks a lot!
Who suggest these pictures?
In the attached photos we can observe 2 microscopic fields from a PB smear of a 27-year-old woman that went in the emergency room with a serious breakthrough bleeding. In addition, she had many bruising in many parts of the body. CBC showed HGB 127g/L, WBC 23x109/L and PLT21x109/L. The doctor on duty at night was not a laboratory hematologist, so he asked for help from a skilled collegue that after seeing the slide alerted the emergency room of the seriousness of the case.
First, I'm using Ficoll Histopaque centrifugation to obtain mononuclear cells. Then, I'm doing RBC lysis. I observe a drastic loss of CD138+ cells after that (as checked using FACS and two separate validated CD138 antibodies). E.g. a sample that contains 18% plasmocytes, has practically no plasmocytes left after Histopaque isolation.
I want to use MACS CD138+ Microbeads but they fail since the Histpaque-isolated cells are devoid of plasmocytes.
Does anyone have such problems? What would you recommend? I've read about autoMACS Pro being good for such cases, but I don't have access to this device.
Since automated hematology analyzers use staining with nucleic acid specific dyes to detect immature platelets I am curious to learn whether these dyes would also detect nucleic acids from pathogens associated with platelets. I know of a case where Babesiosis was originally overlooked due to a high reticulocyte count and assume that what can happen for red blood cells could potentially happen for platelets as well. Differentiation between immature platelets and "pathogen-associated" platelets might be of particular importance in diseases like autoimmune thrombocytopenic purpura. It could help our understanding of platelets as innate immune cells if immature platelets could also represent "pathogen-associated" platelets.
I was asked to supervise my junior in a small project about Chick hematology, because I am new to the topic I have several questions to ask :
1. My juniors are planning to see the garlic oil effect on the chick's white blood cell counts, is it possible to apply oral gavage on chick as the enteral administration method?
2. Is there any suggestion on substance that affect overall blood cell count that doesn't need oral gavage administration like syringe usage ? we are prohibited from using heavy metal
3. If you have any reference about chick hematology-related research please let me know
I have a silly question about the OR. I conducted a study about adherence on oral oncolytics and found that the presence of a solid tumor (OR = 2,026; 95% CI 1,058-3,880; p=0,033) and cyclic dosage regimen (OR 9,457; 95% CI 3,909-22,877; p=0,000) were significantly associated with optimal adherence.
So does this also imply that a hematological tumor and a continuous dosage regimen are significantly associated with non-adherence?? If so, than the ORs for these variables are 1/OR?
Bone marrow aspiration of a 84-yer-old man with anemia (HGB 100g/L), leukocytosi (75x109/L) and thrombocytopenia (47x109/L). Blast cells in PB 37%. In the attached photos some microscopic field of PB (1) and of bone marrow (2-3).
I would like to discuss about this case with RG community
Person who has Vitiligo is permanent deferral for donation or can donate blood for the use of blood transfusion in needy patients .
In the attached photos we can see some cells having similar morphology that in a 65 old years man with middle anaemia (90 g/L) were 23% of a total 5200 lymphocytes. What suggest these?
Because I know the final diagnosis, this is a further contribution to the discussion and sharing of professional experiences in haematological morphology.
Please share me the research if you come across on exogenous enzymes supplementation to fish feed and fish haematological status.
Should clinical haematologists put their microscopes on E-Bay? (van't Veer M, Haferlach T. Haematologica, 2014).
I think no. The ability in recognizing cellular morphology in peripheral blood and in the bone marrow smears is a crucial step in the diagnostic process of haematological diseases even today. Unfortunately we're losing this expertise also because we are unable to transfer our experiences to new generations of professionals. ResearchGate is a formidable place of sharing and exchanging experiences. So, I invite the morphologists to participate in a kind of forum like "images on haematology" with exchange of images, comments and clinical information regarding haematological cases of interest.
I start with images of extreme dysgranulopoiesis in a case of AREB-1
The CBC test results for some patients show a mild to a moderate decline in MCV, in spite of the normal levels of Hb, HCT, and RBC count, indicating there is microcytosis of erythrocytes without anemia. We see this pattern of CBC results more frequently for young males, some of them showing fairly high Hb concentration. What is your practical approach to deal with these results?
Can someone please provide the reference sequence for the coagulation factor 8 gene? It is located on the long arm of the X chromosome (X-q28). I am trying to allign some primers to detect inversions in intron 22 and intron 1 which are responsible for the development of severe haemophilia A.
I would like someone to help me in order to analyze length telomere. I followed the lkit instructions but I do not get a good separation between cell populations by flow cytometry.
When I incubated opsonized erythrocytes and monocytes (monocytes and erythrocytes from the same human donor), the phagocytosis level was lower (11.8% of ingesting erythrocytes), than when I incubated monocytes and opsonized erythrocytes from different human donors (26.64% ingesting erythrocytes). When I incubated nonopsonized erythrocytes (uKKcz) with monocytes (used 24h after isolation) the phagocytosis level was low (0.2% intgesting erythrocytes). When I incubated nonopsonized eryhrocytes (uKKcz) with monocytes (used immediately after isolation) the phagocytosis level was higher (7.46% ingesting erythrocytes). Does anyone know how to interpret my research results of erythrophagocytosis? Any suggestions?
When I analyze CSF by DxH Beckmann Coulter, regularly get higher RBC count in comparison with manually counted RBC. TNC values are comparable in both way of counting.