Science topic
Happiness - Science topic
Highly pleasant emotion characterized by outward manifestations of gratification; joy.
Questions related to Happiness
Background Summary:
In the pursuit of human progress, science has undeniably transformed the material aspects of life—advancing our health, wealth, and technology. Yet, there remains an uncharted territory: how can science be leveraged not just for physical abundance but to elevate the very essence of human existence, fostering prosperity, happiness, health, and even spiritual fulfillment? Can we scientifically engineer a model of living where every individual experiences bliss, vitality, and peace, while also nurturing the collective well-being of society?
The concept of "blissful living" is deeply rooted in ancient wisdom, yet modern scientific advancements in neuroscience, quantum physics, psychology, and social behavior offer unprecedented opportunities to explore this ideal. How do the principles of neuroscience—shaping our understanding of the brain and emotions—align with quantum physics' potential to transcend the limitations of material reality? How can social sciences bridge the gap between individual flourishing and collective harmony?
This vision calls for an integrative approach that combines the best of both the material and spiritual dimensions, enabling us to understand and cultivate abundance, health, wealth, and happiness not as separate pursuits, but as interconnected aspects of a higher state of existence. A holistic, scientifically grounded pathway to blissful living could revolutionise how we approach human well-being on a global scale, offering a framework for not just surviving, but thriving in a way that fosters a deeper connection to self, others, and the divine.
I've tried DMSO before, but it doesn't work with the high concentrations of beta glucan
if you have any suggestions, I'll be happy to know it
How feasibly can I pay a senior academic to review my independent research, then award me a certificate for my independent research?
I have repeated-measures data, but due to missingness, I plan to use linear mixed models. My only predictor is Time, and my outcome variable is a Happiness Score measured quantitatively. The goal is to determine whether there is an overall change in happiness over time.
The happiness score was collected through quarterly surveys at four time points, with the following participation numbers:
- Time 1: 25 participants
- Time 2: 54 participants
- Time 3: 70 participants
- Time 4: 120 participants
Due to substantial missing data, nearly 80% of the responses are incomplete. Many participants attended only one time, particularly at Time 4, which lacks follow-up data. To address this, I filtered the dataset to include only participants who attended at least two time points. After filtering, the participation numbers are as follows:
- Time 1: 21 participants
- Time 2: 35 participants
- Time 3: 46 participants
- Time 4: 47 participants
This adjustment reduces the missing data to approximately 36%. Is it appropriate to exclude participants who attended only once? And how much missing data is OK?
I appreciate any feedback or suggestions.
I'm conducting a study measuring happiness across 4 time points, aiming to determine if there's an increase in overall happiness. The required sample size is 24 for four time points and 28 for three. However, I need help with participant retention. Here's the breakdown:
Survey 1: 24 participants
Survey 2: 36 participants (8 returned from Survey 1)
Survey 3: 60 participants (24 returned from Survey 2, none from Survey 1)
Survey 4: 100 participants (some overlap with previous surveys) To analyze the data, I'm considering only those who completed at least two surveys from Surveys 2, 3, or 4. This resulted in 36 participants and about 20% missing data. I've excluded Survey 1 participants due to more than 50% missing data.
My questions are:
Is it statistically valid to include only participants who completed at least two surveys from Surveys 2-4? Should I impute missing data and use repeated measures ANOVA, or would a linear mixed-effects model better handle the missing data? My model is straightforward:
Happiness score is a dependent variable, and time is an independent variable.
Any advice would be greatly appreciated! I appreciate any help you can provide.
I am doing research that can shed light on what held muslims back, during their Golden Age ( 7th to 9th century BCE) from using their scientific insights and mathematical knowledge to develop innovative products. I suspect that it has something to do with the way they understand what "scientific knowledge" means. It appears to be different from what it means (and meant) to Europeans when they first received the same knowledge. (Ironically, they received quite a lot if it from the Arab manuscripts on Math and sciences that entered Europe through Spanish schools). Both received the same knowledge, and understand it equally deeply. Yet Christian Europe ran with it. The muslims simply sat on it. I want to explore the reasons why. Will be happy to connect with anyone who can point me to the right research and literature. Thank you.
they are rounder and more fluorescent compared to the nuclei of erythrocytes. since I did the study for a research on toxicity, they may also be malformed erythrocytes, but they are too many. if there are professors who have an idea, I would be happy to be informed. thank you.
Dear Members,
I want to overcome the warning at SAP2000 regarding maximum number of null steps. I have several load case and only for my load case Non-Linear Pushover Analysis in X-direction I get this warning.
If I run the Non-Linear Pushover Analysis in Y-direction, I get no warnings. What can be the problem in your opinion?
Few details regarding my model:
-3 Span Hollow Box Girder Bridge
-2 Fiber Hinges at the top and bottom per column
-Fiber Definitions are Default from Section (not created from Section Designer)
As I said I only have a problem in NL Pushover X-Direction. Thats why I dont really think that fiber hinge properties are the problem here. Of course I can be wrong, this is my first analysis using SAP2000.
I am very happy about any helpful responses.
Life is a unique journey toward self-discovery, world awareness, transcendental quests, and universal dreams and struggles. With its metaphysical depth and chaotic yet harmonious nature, human existence is characterized as an exceptional gift, a layered mystery, a cosmic expression of joy and sorrow. Defining the essence of life is just a wave in the boundless sea of subsistence: It is an intrinsic necessity to pursue the meaning of life, to find the essence of being, and to follow the path that leads to achieving it.
Many philosophers and renowned thinkers have tried to decode the meaning of life, enriching metaphysical perceptions of essence, challenging cognitive beliefs, and fostering deep ideas. According to Aristotle, the meaning of life is expressed through happiness, while Immanuel Kant believed that happiness comes from moral duty and the pursuit of the greater good. Philosopher Ralph Waldo Emerson presented his beliefs about the meaning of life through these words:
"The purpose of life is not to be happy. It is to be useful, to be honorable, to be compassionate, to have it make some difference that you have lived and lived well."
In your perspective, what is the meaning of life?
Dear all, I would like to report a fake conference and predatory behavior.
I received several emails from committee@eu.esmed.net , asking to join a conference https://esmed.org/conferences/2022assembly/.
However when I asked for a name I received a named as Dr. M Osman, no first name nor researcher information. I asked for a phone call, and received the email below saying it would be difficult. Asking further, I was asked to join and pay for the conference.
I did not pushed my interaction further, as I first checked on the internet about this society. As I feared, it is a scam attempt. Some people have lost money in the process, as reported in the website below, so be please careful.
I reported my interaction to the human ressource department of my research institutions, as I fear other researchers may be targeted.
However, would you know if, our research institutions or us as individual scientists, have any way of eventually take legal action against such unknown / shabby organization ?
Here is an exemple of the mail I received
Dear Dr. Germond,
Thank you for your email. I am Dr. M Osman. I have served on the ESMED advisory board for several months and am helping with organizing the sessions.
I am traveling currently and with several time zones between us it would be difficult to schedule a call. Perhaps email communication will be the easiest.
More details about the conference are available via the link:
I would be happy to answer any questions you may have.
Could I ask for more details regarding the presentation you may have in mind?
Best Regards,
Osman, M.D.
European Society of Medicine
ESMED General Assembly 2022
Should you look for happiness or opt for pleasure? And why? What are your experiences in the same? Have you felt the difference?
English philosopher Jeremy Bentham(1748–1832). His main axiom, in utilitarianism, was that all social morals and government legislation should aim for producing the greatest happiness for the greatest number of people.
Hi, is anyone familiar with tools that can integrate metabolomics and I6S microbiome data analysis that can help to show correlated pathways and microbial changes influencing a trait? Am happy to collaborate with such a person, as I have both data that am trying to make sense of?
I obtained cellulose from agricultural waste and synthesized its derivative, cellulose acetate. I used glycerin and PEG 400 to obtain biofilm, but I could not obtain biofilm with either method. I tried changing the glycerin and PEG 400 ratios between 5% and 20%, but my films did not dry and still remained viscous. I wonder where the problem might be, I would be very happy if you could help me. Enjoy your work.
Qubit gives this error after reading second standard. It asks to read the standards once more or you can close and continue reading the samples but I am not sure if we can use the results. I could not find a detailed explanation in the user manuel. I would be very happy to hear your experience.
Thanks,
Sema
Cure of cancer?, respectfully to whom it may concern:
If I found a cure of cancer, I’m wondering what I should do with it? I’m just coming out of a 40 days fast of only drinking maple syrup and pure lemon juice plus a little cinnamon powder and nothing else since I had unimaginable pain in my colon end. I figured out that although we humans can last for weeks on this diet /fast, cancer has no food on that and just goes away and the pain is gone too. I received this prescription by tuning in into the HeilstrOm and I figured out that it also enhances the reception of the HeilstrOm greatly, which brings great happiness too, as I describe in my book
Book yogapsychologie
Best regards, hope this helps someone
Chris K. Frueh
Independent Researcher
Having a man love me and want to have sex with me is necessary to my happiness. It gives me a feeling of being worthwhile if I can turn a man on. (Shere Hite)
using book 1 and 2 of Nicomachean Ethics, do u think virtue is essential to happiness?
Hi everyone,
can anybody help me with remote/ distance postdoc positions in Biomechanics / Sports Science?
Since COVID-19 where a lot of the academics and research have shifted to (and still are) online and distance mode, I wonder if there are postdoc positions available without the restriction of being physically present in the lab/ university.
I would be very happy for any suggestions or links to open position advertisements can help me find a new postdoc position.
here we think , always think high GDP mean happy living life of simple man of any society but in some areas of the world simple man life is above average or maybe good still those have GDP issues...
Some studies suggest that certain mechanisms, such as spending money on others, are equally effective in boosting well-being for both the rich and the poor. The rich, however, have more material resources to spend. Additionally, the quality of social relationships is a strong predictor of happiness, with individuals of higher socioeconomic status typically having better relationships than those of lower status. Lastly, the ability to choose how one spends their time is a key predictor of happiness. While wealthy people may still work long hours, they likely have more control over their time compared to those with less wealth. Higher-quality social relationships, the ability to spend money on others, and greater autonomy in time management may collectively explain why higher income is associated with greater happiness. What is your opinion on the role of wealth in happiness?
I am working on a literature review of studies that systematically compare at least one case of collaborative governance to one or more cases of conventional governance (or that compare multiple cases on a spectrum from conventional to collaborative). So far, I have found only 15 such studies, listed in the attached slides. Do you know of others? I'm happy to share my preliminary results, which I'm presenting at IASNR in Cairns, Australia, June 27, 2024. Please let me know of any relevant empirical research I'm missing.
Dear Participant!
I am PhDr. Alzbeta Simon, a PhD student at Selye János University. My dissertation is titled "The Impact of Technostress on Organizational Functioning." Under the guidance of Prof. Dr. habil. Andrea Bencsik, we are conducting a study aimed at examining organizational technostress. For this survey, we have adapted the original questionnaire by Tarafdar, Ragu Nathan, and their research team. Please answer all the questions. Participation in this survey is voluntary and anonymous, and the data will only be presented in aggregate form, based on the research evaluation criteria.
We sincerely thank you for your time and attention in answering the questions, which is essential for the success of the research. If you are interested in the results, we would be happy to send you a summary study upon the conclusion of the research. Once again, thank you for your assistance!
Best regards:
PhDr. Alzbeta Simon
The Research Team
UJS
Participant criteria:
- full time employee, employer, owner, who use digital device in their workplace
- We are looking for responses from individuals who who use digital tools in their work and work in different organizational roles, including employees, employers and owners."
- Users of the SurveyCircle.com research platform will receive SurveyCircle points for their participation.
"The questionnaire is based on the original survey by Ragu-Nathan and Tarafdar (2008) with our modifications. Technostress is stress resulting from the use of technology, the study of which is key to understanding its impact on job satisfaction and organizational trust.
For SurveyCircle users (www.surveycircle.com): The Survey Code is: BKLY-H7Q4-9PCM-46M2
Dear Professor(s),
I am looking for Post-Doc position or visiting researcher position on funded project based. If any professor has open position for international students, I will be happy to join as early as possible.
Thanks in advance for positive response.
Regards,
I'm following the thermal method, as per literature, by putting 3g of melamine into a crucible and heating it at 550 C for 4 hours. Almost all of the crucibles give burnt black Ash while at times one of the crucibles gives pale coloured ash (which is the requirement). I've been doing hit and trial but nothing is working for me. If anyone has worked on a similar project, I'd be happy to have suggestions from them. Thanks
Respected researchers.
Our team would be happy to work with anyone who is interested in contributing to the writing of a single review paper on veterinary homoeopathy.
The collaborator ought to be sincere and have some writing experience in the scientific field or publishing. You can directly message me on ResearchGate or email me at dr.abrar79@gmail.com with a brief introduction about yourself.
Warm Regards
Dr. Abrar
Scientist, Department of Medicine
GADVASU, Ludhiana, Punjab
This barrel or vase-shaped stony structure was found in the Cretaceous shales.
Its outer surface was surrounded by loose and thick limonitic and clayey sheets before I removed them. After removing the loose cover, the main core was revealed inside in a polygonal and cylindrical barrel shape with an outer surface covered by a very thin clay sheet. The terminal ends are indented, too. At first glance, my impression of a nodule-like structure, although I have never seen such a relatively regular anatomy in a nodule.
I would be very happy if I could have your scientific opinions in this regard.
+2
Changing your mindset will certainly contribute to financial success and wealth accumulation, that includes becoming a billionaire solely through mindset shifts. Adopting a positive and growth-oriented mindset can help individuals seize opportunities, overcome challenges, and cultivate the habits of successful people, that also includes a combination of factors such as innovative ideas, strategic decision-making, hard work, persistence, timing, and sometimes luck (which is also controlled through the sub-conscious mind). Mindset plays a crucial role in driving actions and behaviours that lead to success, but is it the only one piece of the puzzle in the journey toward extreme wealth?
How can personalized lifestyle modifications, including diet, exercise, stress management, and sleep hygiene, be integrated into conventional medical treatments to optimize health outcomes and potentially reverse or manage chronic diseases or any other diseases in the past, present or in the future?
My best strategy is to make my body of work on metaphysics so big and rigorous that, people will ponder "how would he have done this without a doctorate?"
1)
Data Metaphysics BA
2)
Data Metaphysics MA
3)
4)
Data Metaphysics PHD
5)
We all know that the long life and happiness of millions need agood diet .health and energy ,and therefore the education and how to neducate people of how to treat our body to keep health and long life
I am a PhD student working in Exp. Condensed Matter Physics. I am working on some Hall Resistivity data. I have few question regarding the Anomalous Hall Effect.
1). How I will know that AHE is present in my Hall Data ?
2). If it is present, How I can extract it ?
I would be happy to if someone explain it for me.
Dear Colleagues,
Have someone access to the Supplemental Material at http://link.aps.org/ supplemental/10.1103/PhysRevLett.120.265702 for Raman spectra and x-ray diffraction patterns at ambient pressure; Results of Le Bail refinements on powder samples; Simulated Raman spectra of Rutile-type SnO2; Raman spectra of compressed Merck sample using methanol: ethanol as the PTM; Pressure dependencies of the Raman peaks in the case of the single crystal; Comparison of Raman spectra at high pressure and after pressure cycle for all experiments; Phonon dispersion curves for rutile and CaCl2-type structures, which includes Refs. [4,5] and [15]. [15] W. H. Baur and A. A. Khan, Acta Crystallog.
I would be happy to have a PDF file.
Best regards,
Rainer Thomas
I am curious if we, the epidemiologists and public health physicians, could think of modified methods using AI in future research some of those may be challenging the traditional epidemiological study designs.
I will be happy to welcome suggestions and ideas in this context, and to perhaps write an article together in this context.
views from the above 3 and why ? What do you use out of the 3 tools or a combination of all .
Would be happy to get views
Dear colleagues,
I have confirmed that people are beginning to use ChatGPT to do their NIH reviews. I believe this is a dangerous precedent given its well-known errors, biases, and lack of original thought. I’ve written a letter to NIH regarding these concerns and would welcome your input (please use “suggest” mode) and signatures. Please feel free to distribute this note to others who may find it of interest. I’m also happy to discuss whether to send this just to NIH or jointly, as an opinion piece to somewhere open with a reasonably high profile, like JAMA.
The current document can be found here:
Greg
Dear colleagues,
It's a pleasure to announce the Call for Papers and Submission Human Flourishing for Wellbeing in Society, Communities and Organizations
This Call of the Journal "Community Notebook. People, Education and Welfare in the Society 5.0" intends to delve into Human Flourishing. This concept has been affirmed in the social sciences for some years now. It tries to respond to a complex historical moment and the practical urgency of offering a transdisciplinary reflection that puts the person as a reflexive actor of his acting in the world at the centre (Archer 1995, tr. it. 1997; 2006; Donati 2013).
Visit the Journal website to read the full announcement:
DEADLINE
30 APR 2024:
- Abstract submission (max 500 words) in Italian, Spanish or English;
- 5 keywords (in Italian, Spanish or English)
15 MAG 2024: Confirmation of abstract acceptance
30 JUN 2024: Submission of papers
15 JUL 2024: Notification of referee results
15 SEP 2024: Revisions and changes to papers suggested by Referees
30 SEP 2024: Editing
For more info, don't hesitate to contact me at mc.deangelis@unilink.it
Clinical > book. Therefore, if a barber was trained and had a track record of successfully giving a very specific surgery then, is more likely to succeed than an MD who only has read books without clinical experience.
If I asked you, ‘What do you want in life?’ You tell me like, ‘I want to be happy, and I have a wishful job and a great life partner with an awesome family.
If I ask you another question that you never think about, ‘What pain do you want in your life? What are you willing to struggle for?'. This is a very interesting question that can turn your life.
Everybody wants to have an awesome relationship, great love, and to be famous. But not everyone is willing to go through the tough conversations, the awkward silences, the hurt feelings, and the emotional psychodrama to get these.
Everybody wants to have an amazing job and financial independence, but not everyone wants to suffer 7–10 hours of work per day, obnoxious paper work, and a daily commute. People want to be rich without the risk, without the sacrifices, and without the delayed gratification necessary to accumulate wealth.
In today’s world, we think of this pattern:
‘I wanted the reward, not the struggle’
‘I wanted the result and not the process’
‘I was in love, not with the fight; I want only victory in life’
'Gain without pain'
What determines your success isn’t “What do you want to enjoy?” The question is, “What pain do you want to sustain?” The quality of your life is not determined by the quality of your positive experiences, but by the quality of your negative experiences. And to get good at dealing with negative experiences is to get good at dealing with life.
Everyone in this world wants to be happy. People want a job, a life partner, love, money, and so on for happiness. But when they fulfil one of these, they aren’t satisfied, and they start to find their new and endless willingness in terms of happiness; they want more and more. But believe this: actually, they didn’t get happiness; we have a misconception of how to be happy.
Happiness is a practice of awareness and dealing with negative experiences. Happiness requires struggle. The positive is the side effect of handling the negative.
What we get out of life is not determined by the good feelings we desire, but by the bad feelings we’re willing and able to sustain to get us to those good feelings.
The landing process of the AFM (NT-MDT) we use does not work. Although the computer program appears to be running, but it does not come close to your fingertips. We cannot observe any mechanical movement. Be more than happy to help with this.
Specifically, I am currently doing my research on the impact of school bullying on the level of self-esteem and subjective happiness in adulthood. Thank you in advance for any help. :)
So I've got high purity 5-MeO-DMT Freebase oil, tested at 99+ by GCMS.
Regarding 5-MeO-DMT, I've spoken with my colleague (our head chemist). We've got the actual synthesis optimized quite nicely now, however the problem is that the end product is an oil (freebase). We've tried so many different techniques to induce crystallization but nothing works. We've found literature that details different polymorphs of DMT and 5-MeO-DMT and indeed some of these polymorphs are oils. The purity by GCMS of the material is extremely high and no residue is left on vaporizing. If you have any suggestions to induce crystallisation I'd be happy to hear. We've tried vacuum distillation as per Shulgin but that doesn't work. The HCl salt is easy to form by I can't get the Freebase to crystallize.
How would you start your own accredited university?
HHappiness is the target f millions ,and nowdays two wars in Ukranion and Gaza affect economy and life of the whole world,How can we end boths?
Hello Everyone,
Have you ever encountered this problem while launching the program HSIMM for ELISA Microplate Reader " Unrecognized database format " and the path into the data file? and how to deal with it.
And please I would like to ask if it’s possible to send me the user manual for your Microplate Reader MB-580 Model if you do have it of course.
Thank you in advance for your answers
Happy New Year 2022
I recently used ChatGPT to rewrite some sections of a paper. It helped me speed up the more technical sections, as repetition is something hard to avoid when English is not your first language (at least for me).
I was happy to use it, but then I realized that it might diminish my credibility when people read that AI was used in the publication. Am I overthinking it?
What do you guys think?
I hope this letter finds you well. I am honored to invite you to be a speaker at International Seminar on “Emerging Trends In Humanities & Social Sciences Research -2024”.Our event is to be held on 11th February 2024 in the Virtual Mode. We know that you are a terrific speaker and our attendees and delegates will gain much from your talk.
International Seminar on “Emerging Trends In Humanities & Social Sciences Research - 2024” is one day event being hosted by Baba Khetanath Mahila TT College, Bhitera, Behror, Rajasthan. We expect our audience to be Indians. Our goal is to bring researchers together from across the globe, to give them inspiration about the importance of research, which we hope will help our attendees.
We do not give any remuneration for speaking engagements (Guest Speaker).Let me be very clear to you in this regard. I shall be happy if you very kindly engage the session for half an hour or so.10 AM(Indian Time) is the time for commencement of seminar. Pls be in time. Thank you for taking the time to read our invitation, and I very much look forward meeting you at the seminar at 10 am Indian Time.
📷Thanking You
Sincerely yours
📷
Dr. Neeraj Kumar Sharma
Seminar Organizer
International Seminar on on “Emerging Trends In Humanities & Social Sciences Research - 2024”
Poonam Academic Research Foundation, Prayagraj
(Agency for conducting Seminar) &
Associate Professor, Department of Management
Singhania University, Rajasthan, India
Mobile no. :- +91-9756966938 (This is Whatsapp number also)
Email :- poonamshodh@gmail.com
Hi everyone,
I analyzed some samples by LC-MS/MS, and I got a pretty good peak for quantifier ion, but the peak intensity for qualifier ion is not good enough so I got "ion ratio" as outlier reason.
My standards calibration curve (in blank matrix) is quite fine and I just have this problem with my samples:
1. Can I consider these samples positive (contaminated) for these analytes?
2. How can I solve this issue and what is the reason for that?
I would be more than happy to hear your thoughts.
Hello everyone,
I am currently working on a Thesis about the impact of AI on Consulting firms.
I am looking for datasets surrouding this subject. If you have any data or somewhat that could help me, I would be very happy to receive your help.
Thank you very much,
Thibaud
Dear all,
I will collaborate with Frontiers in Chemistry (https://www.frontiersin.org/journals/chemistry) as a Guest-Editor for an article collection around a theme of "Five-ring heterocyclic compounds as anticancer drug candidates".
I need Guest Co-editors (3-4 max) who meet the following requirements. If you are interested, I will be happy to work together. Please contact me at arfmermer@hotmail.com if you are interested.
• All members must have a Ph.D. and 3 years of post-doc experience, plus editorial experience
• The majority of the team should be at Associate Professor level or above, with at least one senior member (>50 publications)
• The team should be from a geographically diverse range of countries and institutions to avoid conflict of interest
• Active researchers in the field of Chemistry
Wellbeing and School Curricula
Why isn't psychological well-being (PWB) a core component of secondary school curricula?
I'm very curious to hear input from educators and policy makers on the above issue. Or from anyone reading this.
Is it because:
1) Secondary school curricula are traditionally focused more on intellectual growth than emotional growth. Parents are regarded as the source of education on emotional growth.
2) Educators and policy makers are not aware of the increase in rates of depression among teenagers globally.
3) Educators and policy makers are not focused on student depression / anxiety as they don't think it affects academic performance.
4) A lack of data on the impact of student wellbeing on student performance, resulting in skepticism that changes in curricula may have an impact on student wellbeing.
5) A different reason?
Hi Research Community
I want to start writing a paper. My field of interest is marketing, advertising and advertising strategies and their impact on the behavior of prospects. If you intend to write an article, I will be happy to take advantage of your contribution as co-author in the paper.
All the best
What are the possible consequences of an unethical act? Why? How? My answer: 1) The unethical actor gets what is deserved. 1.5)The unethical actor continues the patterns until getting what is deserved. 2)The unethical actor lives in fear until getting what is deserved. 3)The unethical actor is punished in the afterlife. 4)The unethical actor at some point repents. 6)The unethical actor’s own happiness is damaged by having committed the unethical act. 7)The unethical actor creates a precedent damaging self interests. 8)Something else.
For many years, in punctuated equilibrium theory in public policy, which is a borrowed metaphor from punctuated equilibrium theory in evolutionary biology, https://www.researchgate.net/publication/227675536_The_Evolution_of_the_Theoretical_Foundations_of_Punctuated_Equilibrium_Theory_in_Public_Policy several political scientists used the statistical kurtosis and l-kurtosis to claim that public policy punctuations exist. Recently, numerous peer-reviewed articles have concluded the kurtosis or l-kurtosis approach is sub-optimal in terms of measuring public policy punctuations. https://www.researchgate.net/post/Does_a_Kurtosis-like_Small_Big_or_In-Between_Change_in_a_Public_Policy_Mean_the_Public_Policy_Was_Actually_Punctuated
The Gini coefficient has been proposed as a much better approach to determine punctuations in public policy. https://www.cambridge.org/core/journals/political-analysis/article/why-we-should-use-the-gini-coefficient-to-assess-punctuated-equilibrium-theory/B137B4E6735086498405163E0D208EDE However, can the Gini Coefficient determine if significant and sharp policy punctuations exist? Furthermore, can the Gini Coefficient differentiate between small, in-between, and significant policy changes?
Historically, the Gini Coefficient has been used to measure wealth inequality. The Gini Coefficient is a relative measure. Relative measures are "…a context-specific assessment approach in which the value of an individual score depends on its comparison to other scores within a group or subgroup." https://dictionary.apa.org/relative-measurement The Gini Coefficient for public policy punctuation does not provide a *context* as to whether actual large and sharp actual policy punctuations exist over time. Like kurtosis and l-kurtosis, a small, in-between, or significant policy change can be measured using the Gini Coefficient and can erroneously be described as a large and significant policy punctuation.
Fortunately, a methodological approach has been recently confirmed to rectify this in the peer-reviewed article, Punctuating "Happiness": Punctuated equilibrium theory and the agenda-setting of the Gross National Happiness (GNH) policy in Bhutan' published by Uygen, Givel, and Chophel in 'Review of Policy Research'
The authors of this article conclude that in conjunction with an approach like the Gini Coefficient, a qualitative assessment also needs to occur that includes the following: "These benchmark methods to assess punctuated equilibrium include determining the exact amount of policy changes that indicate policy punctuation; second, the question of scale, policy significance, and impact; third, determining the period required to characterize policy change; and fourth, means of measuring punctuation. Regarding the means, we find that a stronger and more robust method of measuring punctuation is by triangulating possible upticks in media communications about the punctuation with an assessment of the exact nature of the policy change as determined by examining laws, constitutional provisions, etc. We argue that all determinations of whether a policy is punctuated (past and present) must meet this four-prong methodological test."
Triangulating the Gini Coefficient with this four-pronged qualitative approach is a promising way forward to assess and demonstrate significant and sharp punctuations in public policy.
My research design is phenomenology. Good! I conducted 33 in-depth interviews (IDI) and 11 focus group discussions in the field. A total of 95 people participated in the FGD. I have no plan to do a mixed-methods study, so there is no need for quantification. However, I have been told to present the percentages of the number of participants who said what and what. Is the calculation of percentages normal in this type of work? If normal, how do I go about it?
I will appreciate your response. I will also be happy if your responses come with some references where this kind of situation has been presented. Thanks.
I am currently working on MTT assays for determining the molecule IC50 on cells. Based on the MTT results, I want to determine IC50 in GraphPad using the raw data "concentration (not log[concentration])-%cell viability". For this, is it appropriate to calculate IC50 by normalizing the min and max Y value as 0-100 without transforming my concentration data to log derivative and selecting the Nonlinear regression->Dose-response-Inhibition-> "[Inhibitor] vs. normalized response" model from XY analysis?
According to the protocol written on GraphPad's website, it transforms the concentration as X=logX, normalizes the min and max Y values as 0-100 and when I select the Nonlinear regression->Dose-response-Inhibition-> "log(inhibitor) vs. response--variable slope (four parameters)" model from XY analysis, it gives the warning "very wide" for the IC50 value. I get the same problem when I follow the above steps using "Concentration-Absorbance" raw data.
Could you also explain which of the equations in the "dose-response-inhibition" section in the picture attachment would be more appropriate for my study? And, which steps should I follow to calculate the IC50 value in GraphPad?
Thank you for your concern and answers, wish you all happy new year :)
Hello,
Hope all is well and wish you a very happy new year,
Simple question, but answers may differ!
Can narrative Theory be applied as a discourse analysis tool in Journalism Studies?
Thanks in Advance
Hi
I have be studying on bone derived MSC and in the begining I did passaging, ICC, freezing cells and defrosting cells. There was no problem but now my MSC cell cultures were infected two times and I could not find the reason.
I have suspected;
1. Last time I forgot to wash my cells before trypsinization but this affects only number of cell that I gained.
2. I have realized I was doing differentiation assay at the same time and cells in diffrentiation media were not affected from infection. In my diffrentiation media I was using gentamycine and in control media I used penicillin/streptomycin (Pen/Strep). Is this the reason or Pen/Strep does not work?
3.I recieved the cells on 6/12/2023 and I passaged them 16/12/2023 and I fed them on 19/12/2023. There was no problem and I would feed and passage them on 22/12/2023 but I have learnt they were infected?
I was checking them every feeding time is that enough or should I check them every day under microscope ? I do not want them they are infected or disturbed.
I will be so happy if you share your thoughts because I do not want to lose my cells because of infection third time?
Thank you
Wishing you all and your family a very happy, healthy and prosperous New Year 2024 !
Dear Friends,
I want you to read section 4.2 of the following paper and comment.
Happy New Year
Hello,
I am running process_radtags 2.53 on a set of sequences obtained from an Illumina NovaSeq 6000 platform at Genome Quebec. They were able to demultiplex the reads already (I'm just doing quality filtering), so I assume that the barcodes were successfully read by the company.
The problem is as follows:
I developed my rad tags using a double digest approach with paired-end combinatorial indexed barcodes. However, process_radtags will not read my files as paired (only as single-end). It was able to read both the sequence files (which are fastq.gz format) and the barcode file I'm using, so I went forward (note: this was a test to get process_radtags to read the files, so I did not include any cleaning or rescue functions). All of the reads returned as ambiguous barcodes, which is odd given that the samples were already demultiplexed from pooled libraries and the barcode file was formatted accordingly.
Here are some examples of the file names for my read files:
NS.1518.002.Msp1_4---Pst1_7.121419-31_R1.fastq.gz
NS.1518.002.Msp1_4---Pst1_7.121419-31_R2.fastq.gz
When I tried this again adding in the -c -q -r functions, I got the error message (Segmentation fault). My current line of code is as follows:
process_radtags -p ./Da_Data/ -b ./Barcodes/Sample_Barcode_Match_Tab.csv -o ./Prophecy/ -c -q -r --index_index --renz_1 pstI --renz_2 mspI
Does anyone know what the issue might be?
If additional information is needed, just ask and I'm happy to provide it.
Thank you!
We have recently utilised Bambanker (BB01) from Nippon Genetics (Geneflow in UK), which is a serum free DMSO containing cryogenic storage medium for cells. PBMCs (5x10e5/ml) were not happy in this medium when stored at -80C (in an Isopropanol container, althouth I know this is not necessary) for ~12 months (viability dropped considerably from baseline and 1 month data, measured using flow cytometry) and thus we have moved them to Liquid N2. Since then I have found out that Nippon Genetics do not have data on PBMCs rather immortalised cell lines. Moreover I am still not sure whether they use viability and/or growth rate to validate its use.
I am writing to find out other peoples experiences of Bambanker. Going forward I plan to place PBMCs into Bambanker, freeze down at-80 then transfer to Liq N2. Does anybody else do this and have they experienced good (>90% viability?). Thanks Adam Wright
I have to start my thesis next year but I have no idea on how does a reseach gets done nor on how to make a thesis, but I know that it's based on a research.
How should I complete my research ? Or how do I even start? Knowing that by the end of my 6th year I should be graduated So in my 5th year i'm starting my thesis
Sorry for my redondance english is not my first laguage.
Ps: I would also be happy with an advice of gold from you to a medical student like me :)
I'm currently differentiating CD34+ cells into macrophages using medium that includes m-csf and flt3. The differentiation works well and takes 14 days to get ~65-75% confluent attached cells in a T75 that look happy but once I lift, the cells lose significant viability, or completely die and the unhappy alive ones do not reattach to wells or glass slides.
I've tried various detachment methods including 5 minutes in cold 10mM EDTA in PBS, accutase, vigorous pipetting or cell scraping.
Has anyone had good success lifting and reseeding specifically primary macrophages ? Are there any imaging glass wells that I could differentiate the cells directly in that would keep them alive for 2 weeks ?
Hi friends,
Do you have academic, research based, and top tire published paper related to Socialism and business practices? I will be happy if you shared.
Hi friends,
Do you have academic, research based, and top tire published paper related to Socialism and business practices? I will be happy if you shared.