Grapevine - Science topic

I am working on some grape Vitis arizonica leaf extractions and after extraction they look great on nanodrop with 260/280 between 1.72 and 2.05 for all samples and 260/230 between 1.7 and 2.10 for all samples. All concentrations also looked good. Although on the gels there was a blob around or slightly less than 100bp which is RNA contamination. For this I was instructed to do an RNase A treatment (I had already done an rnase treatment during lysis). After doing another rnase A treatment post elution I re-precipitated the DNA using sodium acetate and ethanol to remove rnase (centrifuged @4c), then washed the pellet twice with 70% ethanol. Then I ran a gel and it looked good, high molecular weight gDNA (above 10000 bp) with no RNA blob at the bottom. However, the confusing part is my nanodrop now looks horrible on any sample that I treat with rnase A and re-precipitate. I get 260/280 of 1.4-1.7 and 260/230 under 1.0 for any of these samples. I am not sure what contaminant could be there?

Hello to all,

I would like to characterize the bacterial microbiota of soil, root and leaf samples from grapevines. My goal is really to be as exhaustive as possible and if possible to use the same pair of primers for the three compartments. Do you have any experience/advice to give on the choice of a region to target or even a pair of primers in particular?

I thank you for your help

Paola

I work on investigating the microbiome of the grapevines Rhizosphere using Shotgun-metagenome sequencing-Illumina. DNA was extracted using PowerSoil® DNA Isolation Kit - QIAGEN and further purified by Sodium acetate. Metagenomic DNA libraries (50 ng input) were prepared using NEBNext® Ultra™ II Prep Kit for Illumina®. The readouts of Bioanalyzer, Qubit™ dsDNA HS, or 16s PCR look perfect. However, the sequencing run gets always underclustered in case of my samples. Nevertheless, it disrupts the clustering of the other samples, too.

Are powdery mildew and downy mildew vascular diseases?

Hello everyone,

I am going to rear the European grapevine moth Lobesia botrana in the laboratory to study the biology of this moth and test some pesticides. In this time, I got a number of larvae from grape berries but all larvae dead when I feed them on new grape berries or grape juice. Does anyone know artificial media that I can use to rear this grapevine moth?

Thanks in advance for your suggestions!

Kind regards,

Is there a mobile phone application for estimating the number of flowers per inflorescence of grapevine by image analysis?

If possible it should be compatible with Android or similar.

Hello!!

Could anyone point out to me an efficient protocol for microscopy of grapevine shoot tips?

Thank you so much

Is there a database of presently identified grapevine disease resistance loci?

Are pathogenesis-related proteins and antimicrobial peptides encoded by plant resistance genes (R genes)?

I'm unsure if the genes for these proteins/peptides are types of R genes?

Hi,

I want to knock out a resistance gene in grapevine. I am looking for the vectors who are used for grapevine in Golden-gate pathway. Up to know I have expressed the gRNA using two target vectors and I am not sure that I need to make the vector contains Cas9 by myself or it is better to buy it artificially?

I found information for Grape pearls /Sap balls/, but it's insufficient. Does anyone have done detailed research on this phenomenon?

Тhanks in advance!

I need papers talk about Biochemistry relation between Erysiphe necator - which caused Powdery mildew in grapevine - and its host Vitis vinifera .

Should more research go into creating cultivars of non-domesticated plants that can be used for various fibers. As an example milkweed floss was used during WW II in various flotation devices, while the milkweed provides nutrition to developing monarch butterfly caterpillars and it native to the North America. This plant could be better for the environment and local ecology. It can also grow in range of climates. Another example is cottonwood, the fibers or "fluff" could be collected an used. Or develop usages of the fibers from grapevines, there are 60 species native to North America.

I want to study phylloxera and its effects on rootstocks, during my visit to the Technical Institute of Fruit and Vine Cultivation here in Biskra, and I noticed the presence of phylloxera on rootstocks that are known by their resistance to this disease. from these remarks I ask myself many questions which I would like to find the answer. I would like to have your opinion and your suggestions. For that I will be grateful.

Thank you in advance for your answers

Samia Khouni

Or...

How big must be the vineyard to justify the purchase of a grape harvest machine?

To push methacarn fixative into tiny shoot apical meristems (0.05 ml volume) what is the negative pressure that needs to be applied to the dessicator (in mmHg and kPa) and how long applied BEFORE the vaccuum is very quickly released to force the liquid into the tissue?

Is there any newer and more efficient or accurate equipment for measuring the Brix and pH of grape berries than a digital refractometer (Brix) and a pH meter (pH)?

What shell we expect and test plants before founding new grapevine plantations? The list includes 14 viruses: Alfalfa mosaic virus; Arabis mosaic virus; Broad bean wilt virus; Grapevine Algerian latent virus; Grapevine fanleaf virus; Petunia asteroid mosaic virus; Raspberry ringspot virus; Sowbane mosaic virus; Strawberry latent ringspot virus; Tobacco mosaic virus; Tobacco necrosis virus; Tobacco ringspot virus

Tomato black ring virus; Tomato ringspot virus. What are the 3-4 most harmful in your country?

Which is the best protocol to extract RNA from grape leaves?

I will like to measure the effect of temperature on flowering and fruitset in grapevines and I would like to have a protocol to standarize my measurements. Does anyone have done this before?

Thanks in advance

I need your help for my dissertation.

Our research vineyard was hit by herbicide drift.  The symptoms indicate that it may have been several herbicides.  Some of the border rows are badly damaged.  Should we error on the side of caution, and discard the fruit from these vines?  Any comments or research leads would be appreciated.

Dear all researchers!

Can anyone explain me about the calibration of  spectral evolution spectroradiometer for taking the spectral measurements of fruit trees?

Regads,

Ansar Ali

cancer detection

How collect atmospheric CH4 samples for 14C analysis? About 0.5mg carbon is needed for AMS measurement.

The photos were taken in northern Italy at the beginning of September of the current year. The vines are placed in an experimental area and are grown in pots. The lower surface of the leaves is normal. Some entomologists and acarologists exclude that the damage is caused by mites. The damage has a negative impact on the quality of grapes. Did someone note similar damage elsewere and does he know  or can hypothesize the cause?

Dear all,

we are going to use the PAM-2500 to measure chlorophyll fluorescence parameters in grapevine. The measurements will be conducted outside, therefore we want to keep it simple, but of course efficient in data aquisition. Therefore dark adaptation is no option for us, bu the instrument has many different functions to circumvent this problem.

I have the following questions:

1. the field screen with the Y(II) measurment provides no light acclimation step with standard actinic light, just a light puls. do you think a standardized acclimations to actinic light is necessary?

2. Fo´is measured under field sceen after the light sat puls and a short period of FR. Is this similar to the FR+Y option?

3. do you use default light intensities? we are working with grapevine

many thanks

Michaela

The herbicide drift occured early in the season when the vines had 10-30 cm shoot growth.

The metsulfuron was mixed with 2,4-D. The most obvious symptoms on the grapevines were consistent with 2,4-D damage but there were other symptoms as well. I am seeking more images that show the range of symptoms assoicated with metsulfuron, and also interested whether anyone has experience where metsulfuron was applied in combination with 2,4-D.

I am planning to assess the total GAs (also want to assess GA3 seperately if possible within my circumstances) from inflorescence of grape berries and mature berries. I had stored samples at -80 since the day of harvesting. I found an enzymatic kit but didn't find any article related to this.....could you suggest me the best methods if you have experiences eg/ HPLC, kit or any others 

I am doing research in analyses of molecular genetic diversity of grapevine varieties by sequencing method. However, I lack articles with information including methodology of DNA sequencing of grapevine. I request assistance of articles or any documents which can assist.

I am developing a study about young vines cultivated in the soil contaminated by Cu. Plants were inoculated with a different fungi and so alter the gas exchange measured using the IRGA.

Does anyone know any papers about it?

I'm evaluating the application of two products and their combination against Plasmopara viticola. Both products may induce priming of Jasmonic Acid pathway in grapevine. I fear that observation of a methyl jasmonate production after P. viticola challenging very higher than untreated challenged control is not sufficient, mainly because the pattern of this enhanced production is different between the two products. So, I’m would like to evaluate if: i) treatment differentially primes JA pathway in grapevine (before P. viticola challenging); and ii) there is different patterns of activation of marker genes for JA pathway activation among the two treatments .

Thanks

Fabio Osti

I need to do PCR for the detection of Grapevine feathery vein virus.

Does anyone has experience with indirect organogenesis of Vitis vinifera. I mean shoot organogenesis from calli (not somatic embryogenesis). 

I am currently working on a project about DNA Profiling for Grapes/Grapevine to distinguish origin of one grape from another. I am wondering if anyone knows whether there's a profiling kit available for grapes? Any links, scientific papers would be much appreciated. 

Thank You!

28 items of our rootstock collection were modified to a single curtain in 2010 by grafting a scion (Cserszegi fűszeres, white wine grape) with green to green grafting on them.

Grafts were positioned between 50 to about 150cm above ground (same height could not be kept for the differences of lignifications and cultivar characteristics).

With 3 yearly shoot selections on the rootstock, besides the scion stock one shoot of the rootstock is also left growing.

Weeping dew was collected from the first year, but I can find no relevant data.

What is the best grape rootstock for nematode resistance?

Hello,

I am a PhD student in the third year thesis. I work on the measurements of optical properties of fruits. Application to the structural exploration of fruits.

I measure the optical properties by the method backscattering rays outgoing of the sample, ie Spectroscopy Space Resolved and I measure the coefficients of absorption and reduced scattering (µa,µ’s) of apples, pears and tomatoes. Once the optical properties are measured, we try values correlate with the chemical composition and structure of the fruit.

I have read your article entitled: Optical Properties of Berry Epicuticular Waxes in Four Georgian Grape Cultivars (Vitis vinifera L), and you measured the reflectance, however, I am interested in measuring of the optical proprieties of grapes and I have looked your discretionary experimental used in your study was Jaz System (Ocean Optics BV). Personally I do not know this device, could you send me a paper of the operation of this last?

Otherwise, in my thesis I use lasers λ = 633 ,760.784 and 850 nm, the backscattered signal is captured by a CCD camera that will give me an image with lots of distances and intensities, then I apply a Farrell fit method (point source and semi-infinite medium) that gives me the (µa, µ’s).

My question is: is it possible to measure the optical properties of grapes with my spectroscopic method for spatial resolution, if you already have done an extraction algorithm of the optical properties of grapes?

Thank you in advance for your return, I will not forget you your gesture

Best regards,

What type of agar? When to sample the source? How to grow a colony? How old should leaf be when I do the replanting of the colony?

I am looking at different methods to measure/calculate the leaf area of grapevines. I have never calculated the area before and while reading the literature to find the best method I found that there are several available, ranging from measurements of Leaf Area Index (LAI) with calculations based on leaf length/width to the use of portable scanners and even smartphone apps. Any recommendation from users would be welcome.

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Could anyone tell me which tool cold convert them into affymetrix probe ID. Many thanks.

Endoreduplication = increase in DNA without cell division, meaning the cell number stays the same but DNA content increases.

Specifically to estimate the volume of a pruned vine trunk of grapes for CHO and GA assessment.

I am looking to buy an instrument for pressing the juice out of grape samples of small size (up to 1 kg of grapes). As the pressure applies to the grape samples can affect the extraction of the compounds I want to measure, I am looking for tools with pressure control so to be able to standardize the pressing among samples. Any suggestion of suitable instruments? thanks!

There are two main berries sampling strategies used among experiments to investigate sugar, and other quality parameters, response to different treatments (irrigation , nutrients, defoliation, etc.): one is to harvest all treatments on the same date and the other is to harvest each treatment when a targeted sugar concentration is reached. This differences in strategies usually results in different conclusions on same treatment effect. Which one is correct/better?

I am looking for an adjuvent (sufactant) to add to a solution destined for foliar application to increase efficiency of absorption into the leaves of Vitis Vinifera. Any recommendations?