Gastric Ulcer - Science topic

Could anyone help to find the method for stomach ulcer induction in rats according to Shay et al. or published work on it more in detail?

Helicobacter pylori inhabit the gastrointestinal tract, one person’s poison may be another’s cure. Helicobacter pylori, the bacterium that causes gastric ulcers and stomach cancer in some people, may actually protect against cancer of the esophagus. So, What about your experience in the paradoxical effect of Helicobacter pylori infection? and why their resistance to treatment was increased?

please provide me with following article.

Curling in 1842 was the first to describe detail that, Acute gastroduodenal erotions and Gastric ulcers can be associated to burning injuries nomely "Curling's ulceration".

Currently, I’m working with model of gastric ulcer induced by EtOH or NSAIDs (diclofenac). I use ImageJ software for macroscopic scoring to compare the ulcer area.  Unfortunately, I couldn’t find any reliable system for microscopic scoring.

Could you recommend any scoring system to use in microphotographs (H&E staining) obtained from mice stomachs?

In our first experiment we got the average value of 800 µg of alcian blue/ gram of wet stomach in normal control group (when we read at 580 nm in absorbance reader) but in the second experiment with different set of animals at same wavelength and in the same instrument we got the average value of 60 µg of alcian blue /gram of wet stomach for normal control group.

What is the reason for this difference?

Is this normal (experiment to experiment based) observation? Or abnormal observation because of some other reasons?

Is there any specific range for gastric mucosa content in Wistar rats?

Your help is greatly appreciated.

Thanks and regards

Eshvendar reddy

Aspirin and numerous NSAIDs, including acetaminophen and those inhibiting selectively COX-2, are amongst the most commonly consumed drugs on the planet. Aspirin at doses less than 100 mg per day or greater than 1 gm per day have equivalent effects on platelet COX-1 derived TxA2. They both suppress it completely. However, increasing daily doses of aspirin increasingly inhibit PGI2 coincidentally with this effect, which suggest that the cardioprotective efficacy of aspirin may be progressively attenuated as the dose is increased. Similarly, locally formed, COX-1 derived PGE2 and PGI2 are protective of gastroduodenal epithelial integrity and platelet COX-1 derived TxA2 contributes to hemostatic competence. Disruption of these pathways by NSAIDs (even those which selective for inhibition of COX-2) resulted in serious gastrointestinal events. That may reflect their impact on gastroduodenal epithelial COX-2 dependent prostanoids that accelerate ulcer healing. As we still poorly understand inter-individual differences in anti-inflammatory efficacy amongst the reversibly acting NSAIDs, the hard evidence how to address the above question is in short supply.

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I'm working on anti-ulcer and ulcer-healing effects of fruit peels extracts on stress-induced gastric ulcers. In order to test the gastroprotective effect of the extracts how many days should I feed the extracts to rats before subjecting it to procedure of inducing ulcer?

     

please how can can i induce stomach ulcer orally in rabbits and also using biochemical markers to diagnose the ulcer in the rabbits.

Which is more ulcerogenic to the stomach: aspirin or clopidogryl?

30 year old who under went an attempted lap chole converted to open,also underwent a diagnostic Lap 8months ago,presented with complaints of abdominal pain,feels full with small quantity ,no vomitings and no significant  loss of weight.She was hospitalised twice after surgery for pain and was treated conservatively as per her hospital records.OGD in their hospital showed a gastric ulcer and a repeat was said to have the ulcer healed.Clinical exam is normal except Right subcoastal incision and multiple Lap port scars.OGD in our hospital showed the above suspected diagnosis and technically difficult to extract.CECT report is awaited.

I gone through many papers that they calculated the ulcer index in the stomach of a rat model. They gave some score depending on the ulcer's color and size. I don't understand how to give the score and how to calculate the UI. Kindly let me about this or suggest any other method to calculate the UI.

I am working with gastric ulcers and I need a culture of Helicobacter Pylori. Does anyone have any idea where I can get a culture in and around India?