Garlic - Science topic

Olive oil and garlic are not typically considered causes of hair loss. In fact, olive oil is often used in hair care for its moisturizing properties, and garlic is sometimes believed to promote hair growth due to its potential to improve circulation and provide nutrients to the hair follicles. However, if you have a specific sensitivity or allergy to these ingredients, they could potentially cause scalp irritation, but this is rare. Always do a patch test before using any new hair care products to check for adverse reactions.

Hi everyone,

I am working on microalgae I am finding difficulty in maintaining the cultures. After 20 days microalgal cultures are turning brown. I used antibiotics, garlic extracts but not working. I need a suggestions to overcome this challenge.

Which specific species and genera of potyviruses, carlaviruses, and allexiviruses significantly impact the quality and quantity of garlic yields on a large-scale production level?"

According to our work on the effectiveness of water extracts of (Sumac) Rhus coriaria, it was more resistant to Gram positive than Gram negative strains of bacteria.

  What's your opinion on the effectiveness of extracts of Sumac, Garlic powder and Tomato powder for that purpose?

If you did that, how many did you use and what was the rate of removal of foodborne pathogens?

  What's your opinion on the effectiveness of extracts of Sumac, Garlic powder and Tomato powder for that purpose?

Hi anyone can help me to find methods to increase the multiplication of garlic plants in vitro culture. I am using Ms media, NAA and BA solution atm?

I do experiments with garlic in vitro and green house. I observed that garlic trees stopped growing, turned yellow in leave tips and wilted leaves. Is that natural physiology of garlic or abiotic /biotic stresses?

I have got following results can you please tell me my result are fine or not if not what I should do to improve my result. Moreover, I haven't seen inhibition zone in that experiment. 10-6 I have got 160 colony calculated as 0.062 is it correct calculation??

On a proposal I evaluated, a student planned to examine the effects of 3 levels of intra-row Spacing and 4 NPS Rates on Growth and Yield of Garlic. The student designed the treatment structure for NPS rates as follows:

NPS rate (kg ha-1)

10, 29 and 5

19, 38, and 7

28, 47 and 9

38, 57 and 11

As I think the student may use the proportions of N, P and S in NPS fertilizer blend, i.e., 19%, 38% and 7% as a basis to set his treatment structure. I claimed that this treatment structure is wrong and suggest changing it into 0, 50, 100 and 150 kg ha-1 NPS rate, or something like that. But, the student and his supervisor refuse my suggestion.

Dear Professors and colleagues, is it really the correct way of treatment structure? If not, what is your advice for them?

after having a thorough review of some answers i have decided to add the following remark on my earlier question

Remark: The student is not making a physical blend of each fertilizers rather he will use the already blended form of NPS fertilizer.

Dear all,

Good day.

I'm currently working on a formulation involving an extract from garlic, allicin and will be moving onto the in vitro drug release test. Based on my preparation for the standard curve via UV Vis spectroscopy, allicin is temperature sensitive and has to be placed on ice (the absorbance seems to fluctuate if its left at room temperature over time). The drug release assay seems to be conducted at 37 °C most of the time hence, I'm wondering if it would be a problem if I'm planning to measure the release of allicin from my formulation via UV Vis during the in vitro drug release assay?

Thank you in advance for your time.

What are the health or nutritional benefits of Garlic?

Can it boost immune system?

im doing a search on seeing the effect of a mixture contaning apple cider vinegar,ginger,garlic and lemon on the glucose level of the diabetic patient.in control i m taking a placebo.bt im cofused that i either i have to take placebo in liquid form or in powdered form, as i am going to give a mixture to diabetic patient.so plz guide me in this regard.

if given in liquiid form,what should be the liquid form of placebo.at first i was using wheat flour as a placebo.But then my supervisor instructed me not to use wheat in this case,as im using mixture form in the case group.so please suggest me liquid form of placebo

Garlic has anti-viral and anti-inflammatory properties. The garlic extract had inhibitory effects on Infectious bronchitis virus (IBV), which is a coronavirus. (Avicenna J Phytomed. 2016;6(4):458–267.) Garlic compounds reduce inflammation and oxidative stress (Viruses. 2017;9(7):159). Garlic compounds also significantly inhibited the production of proinflammatory cytokines. (Biochem Biophysic Res Comm 2015; 464(3):774-779).

Therefore, is it OK to recommend garlic to people for fighting coronavirus infection, which is associated with increased inflammation ?

i am doing reserch on to investigate the effect of a mixture including ginger fennal seeds, garlic and cinnamon to see how it effect the blood sugar level of a diabetic patient. Ineed a help how to proceede it further. How should i start my intervention. plz guide me completly.like how i determine glucose of diabetic patient etc.Need complete guidance.

Can anyone help me to over come the problem of mixing essential oil and water layer in tubes of Clevenger type apparatus while hydro-distillation of garlic? The oil layer does not remain in top of the water phase and with time, oil layer separates and come down along the water column.

How much and what time?

To obtain the methanolic garlic extract, 200g of garlic powder was added to 600 ml of pure methanolic solvent and gently mixed. After 24 hours, the garlic powder got sedimented and started to accumulate at the bottom of the vial. Eventually, the sediment got harder and it couldn't be stirred properly. Now in this situation, I am worried whether the secondary metabolites will property dissolve in this organic solvent or not!

How can I solve this problem?

I did analysis on DPPH antioxidant on Garlic aqueous extract and standard (vitamin C), so I don't know how to find the IC50 of both. Please I need a very direct guide.

kindly explain it

Effects of short-term garlic supplementation on lipid metabolism and antioxidant status in hypertensive adults. Duda G1, Suliburska J, Pupek-Musialik

Hello, we have been trying to run MSTRAT Software for developing a core collection of Chilean Garlic genetic resources, but we keep getting the error:

ether couldn't execute "C:\Users\VICTOR\Desktop\MSTRATe\ether": invalid argument.

If someone could guide us in what is going on with the program or how to run it correctly, it would be greatly appreciated.

Hello everyone/ Assalamu alaikum!

I am growing garlic in my area (Peshwar, khyber Pakhtunkhwa provice of Pakistan). All the macro and micronutrients are also applied to the field. 40 days after germination of garlic, some of the plants leaves became yellow (started from tip). The same case was observed everywhere in Pakistan. I need to know the causes and consequences of this problem????

Pictures are attached here for understanding the problem

I've heard garlic and high nitrate foods like arugula or beets typically occupy high ranking here. What others do you know of or recommend? What's your top 10 list, based on what research? Thanks.

I have used a mixture of Capsicum, onion and garlic with water and I sprayed it over two lemon trees, rubus and another plant from the Crassulaceae family and all of them produced flowers and fruits in a period of two weeks, thus I am interested in continuing with these studies, but I would like to know if someone is working on this issue from this community, and perhaps we can share information.

i would like to share my personnel experience with garlic use in Otis media with ear drum rupture

i did used fresh garlic crushed & mixed with olive oil & finally only garlic in my problem of ear discharge it was not controlled with amoxicillin clavulanic acid & use of other antibiotics such as chloramphenicol ear drops

would any body lie to extend this work

ZBNF is promoting the usage of non chemical inputs like Jeeamurthram, Beejamruthram, Neem & garlic decoctions and waste decomposer. Whether the usage of these inputs will increase the agriculture production.

We have seen a flood of stories advocating pseudoscientific interventions and practices to help prevent coronavirus. There are suggestions, for example that hot air blown up the nose kills the virus; that eating garlic can prevent viruses; and that pneumonia or malaria vaccination can protect against the virus.

i am working extract of onion ,ginger and cinnamon as antibacterial

any other material for allin rich content

I would like to use spectrophotometric method to quantitative determination of allicin in garlic extracts.

I tried method by Han et al. (A spectrophotometric method for quantitative determination of allicin and total garlic thiosulfinates; DOI: 10.1006/abio.1995.1124) but I didn't get any difference in absorbance between control (l-cysteine only) and my sample.

I'm sure that sample contains allicin because I tried many comercially available garlic extracts.

When I add DTNB into mixture I get colour of Ellman's anion but intensity of the colour is practically the same in control and the sample so I think that reaction between allicin and l-cystein doesn't happen.

I tried time of reaction like 15 min or 2 hours (incubation at room temperature or 30 Celcius degrees) and still don't get any difference in absorbance at the end exept incubation for 2 h at 30 Celcius degrees - I got A=0,127. It gives much lower concentration of allcin then I expected. I used extract with content of allicin I know so I think that this value is incorrect too.

Have you ever had a problem with reaction between allicin and l-cysteine? What conditions should I have to make sure that reaction is done and all allicin I have react with l-cysteine?

I would be grateful for any help! Thanks!

Garlic is a plant for human consumption and it is said to have health benefits.

What are the parameters to be studied during storage of garlic

Due to the variety of compounds present in garlic such as water- and lipid-soluble organosulfur compounds, phenolic compounds, notably allixin, saponins and selenium , garlic has many medicinal and nutritional value. but some of the processing methods subjected with heat may reduce the anti-oxidant values. i want o make sure which and which.

My study on onions and it role as an antihypertensive herbal medicine makes me conclude that it most has muscarinic cholinergic agonist and one subject complains of acute bradycardia and loss of consciousness after an overload onion and garlic herbal product but I want to extract the active components and determine it's composition and I need help

Garlic is very unique for many kinds of treatment for many disease,and found as good for wide applications,something irresistible about the aroma of roasted garlic. It is so captivating with its powerful notes, that it has long been used as a flavour booster in curries, stir-fries, pizza toppings, pastas, meat preparations, dips - you name it! It has the power to instantly liven up any dish and treat some of the most common ailments.  While garlic is a common ingredient in every kitchen, in the ancient times, it was highly valued for its numerous health benefiting properties, which are still followed in many cultures today. Our ancestors have used it as a bug-repellant, Medieval Europe against plague and the Egyptians would even bury it along with their dead! Some benefits of garlic are credited to the presence of the sulphur-containing compound, Allicin, found in fresh, crushed or chewed garlic, due to which it has anti-bacterial and anti-fungal properties. Some startling claims mention that it may help prevent some forms of cancer too. The health benefits of garlic are aplenty. Garlic is a part of the onion family and the 'bulb' of this herb typically consists of 10-20 smaller sections called the 'cloves'. Each small clove is a powerhouse of flavour as well as medicinal properties.so what are the medical use of garlic?

Is there any EU country or state in the US that "officially" approved the use of Allium sativum (Garlic) for the management of primary hypertension? There seem to be a lot of evidence based research that shows the indication for hypertension to be excellent. Has Allium sativum (Garlic) been patented apart from local consumption for food and spices?

Hello, I am interesting to make a research about AgNP antibacterial activity using the green synthesis method for my thesis. I've read from several publications, they use various concentrations. It's my first time to do research with nanoparticles.

Let's say, I will synthesis the AgNP with mixing aqueous garlic extract (1 ml) to 1 mM AgNO3 (19 ml). What I want to ask for help are:

1. I would like to know, how to make the various concentration ranging from 100 µg/ml to 6.25 µg/ml from that AgNP solution?

2. Can I dilute the synthesized AgNP with distilled water? Like when we dilute plant extract.

Thank you very much in advance.

I extracted bioactive compounds from garlic by using n-hexane and evaporated it afterwards. However, the concentrated extract is not fully dissolved as I can see precipatates floating in suspension. I have tried using DMSO, methanol, and ethanol to aid in the complete dissolution of this n-hexane fraction but to no avail.

I searched online for articles about defatted garlic but none of them mention whether they encountered this problem or not. Does anyone have an idea why this is happening and how I can troubleshoot this?

Am working on the extraction and purification of Amygdalin fr apple seeds and Ajoene from Garlic.....how do I go about this using HPLC?

I am evaluating the effects of tumeric and other spices including garlic on broiler performance and carcass quality.

Considering that I work with cell culture and one of my experiments included treatment with garlic oil, I would be grateful if someone can suggest me how to dissovle it. I tried to dissolve oil in DMSO, but it didn't work. Something went wrong, and I had some droplets of oil in my well plates, so my cells died. I suppose that I didn't dissolve oil on adequate way. Maybe I didn't use right concentrations, although I am pretty sure that I didn't make mistake with it. Hopefully, you will give me some short instructions to do that in the right way. Youer effort is strongly appreciated.

I'm using "Use of a Free Radical Method to Evaluate Antioxidant

Activity W. Brand-Williams, M. E. Cuvelier and C. Berset" as reference.

I' m trying to measure antioxidant activity on garlic extract but something react with metoh so the solution isn't clear and can't be read on spectrophotometer .

thanks a lot

C.Orefice

We are planning to study the health benefits of garlic oil in volunteers with diabetes, hypertension, and hypercholesterolemia, The active ingredients of garlic oil are allicin, ajoene and allyl sulfides which can reduce the biosynthesis of cholesterol. So we need to find out the optimal and effective doses of garlic oil used.

In term of bolting garlic may be three types non-bolting, incomplete bolting and complete bolting. In case of complete bolting it may be used for genetic exchange (crop improvement) but what is practical use non-bolting and incomplete bolting garlic genotype genetic diversity study.

Benefits of garlic .. and garlic oil

Garlic is a good source manganese, selenium, and vitamin C, Phosphorous, calcium, potassium, iron and copper.

Hi guys,

I was wondering if smoking fresh garlic bulbs over a smoldering fire (just to give the smoky flavor to the garlic, outside skin turning black but inside cloves remaining raw) can be used as a preservation method to extent its shelf life.

Thank you

Hi, I'm a student researcher who is currently studying about the antifungal activity of ginger and garlic. Any tips for our methodology?

Autumn planting of sugarcane(September to October) reported to give 25 % higher cane yield as compare to spring planted sugarcane in subtropical India. farmers grow different intercrops in autumn planting of sugarcane. Cyperus rotundus is the major weed of sugarcane during the autumn season also. Halosulfuron was applied at 67.5 g/ha for nutsedge control and after its applicxation farmers want to intercrops like cabbage, cauliflower, cabbage, mustard, garlic. Is it safe to grow these crops 60 days after application of halosulfuron methyl.

Sugarcane was planted at 120 cm spacing with an objective to have intercropping of Chickpea, linseed, lentil and garlic. Sugarcane crop was sprayed with Metribuzin ( 500 g/ha) 30 days after sowing to control some weeds. Now the sugarcane crop is of 60 days old and we want to sow intercrops like Chick pea, lentil, linseed and garlic . Is it safe to grow these crops after one month of application of Metribuzin ?

How the taro work is coming under AICRP Onion and Garlic

You can mention you thesis title as project.

Seven miligrams (7mg) of garlic powder was suspended in test tubes, this was performed in triplicates. The contents of all the test tubes were made up to 1mL with DMSO, distilled water, saline solution and ethanol respectively, while another test tube marked ‘B’ with 1mL of distilled water served as the blank. Thereafter, 0.5mL of Folin-Ciocalteu reagent (1N) was added to each of these test tubes and the blank. They were all vortexed well and allowed to stand for 5minutes at room temperature. Then, 2.5mL of 5% sodium carbonate was added to all the test tubes including the blank, vortexed again and incubated in a dark room at room temperature for 40 minutes. A standard solution was prepared according to Makkar (2003). Aliquots standard solution of 0.2, 0.4, 0.6, 0.8 and 1mL were pipetted into 5 test tubes respectively. The absorbances (y-axis) and concentration of gallic acid (x-axis) of the standard solution were used to plot a standard graph (calibration curve).

I have to extract DNA from a large number of garlic leaf samples for SSR analysis. I am doing a bead milling technique using SPEX SamplePrep GenoGrinder. My current protocol involves using lyophilized garlic leaf tissue (around 50 mg), two 3.2 mm metal beads, 2 ml tubes, 1200 rpm for 45 sec with pause every 5 sec to rest tubes in ice. My extraction buffer is composed of 3% CTAB, 100 mM Tris-HCl ph 8.0, 20 mM EDTA, 4% PVP, 3% B-me, and 2 M NaCl. My problem is that all of the DNA that I get from my method is sheared/degraded. Is there anyone who has an experience using the bead milling technique for DNA extraction of fragile leaf tissue?

Thank you in advance.

I'm working on garlic essential oil microwave extraction and I'm having problems with chopping the garlic. I tried using a manual food chopper but it resulted in varied sizes of garlic. Do you any recommendations to use. Garlic compounds are also very volatile so material loss is a problem for manual chopping in ambient air.

I have to perform a study on RAW 264.7 macrophages cell line using a garlic extract. How can that be prepared?

I have incorporated fresh garlic stalk into replanted soil in pot experiment examining cucumber growth and soil quality. Before application tested the soil and garlic residue properties. we found 444.43 C:N ratio of garlic stalk. can you recommend as good quality crop residue? and more explanation at that CN ratio.

I was asked to supervise my junior in a small project about Chick hematology, because I am new to the topic I have several questions to ask :

1. My juniors are planning to see the garlic oil effect on the chick's white blood cell counts, is it possible to apply oral gavage on chick as the enteral administration method?

2. Is there any suggestion on substance that affect overall blood cell count that doesn't need oral gavage administration like syringe usage ? we are prohibited from using heavy metal

3. If you have any reference about chick hematology-related research please let me know

Thank you

I want to know, if there are garlic varieties or types that produce bulblets similarly like tree onion.

i would like to coating tempeh packaging made from banana leaf with garlic extract. but I dont know the way to make it stuck on the leave because banana leave has a wax layer

I need garlic seeds but usually garlic variety I grow do not flower

chemical composition of the garlic and onion essential oil

Could you tell me please, how much is the specific weight of chopped raw garlic and chopped raw onion? Thank you.

Any one who found efficacy of botanical extracts against garlic thrips? please add the details

Gilda Joana, Alok Nahata, Chandan Chanotiya and B.R.Rajeswara Rao

I came across the protocols for preparing AGE and all of them said that the garlic must be kept in ethanol for 10-20 months. But I saw a video on youtube on how to extract AGE and it said, we can put the minced garlic in 96% ethanol for just 10 days to get AGE. Is it fine or should I keep it for 10 months, or should I get the commercial AGE? If in case I it is better to get a commercial one, which manufacturer would you suggest. Thanks for your suggestions in advance.

We would like to produce garlic oil in lab by simple method to compare with commercial one. Which solvent we should use? We do not have super critical extractor.

At the moment, we are using citric and ascorbic acid for decreasing garlic greening. We know that allinase is the responsable of this process, resulting in the formation of blue/yellow (green) pigments. Allinase provide thiosulfinates which react whit free aminoaciod creating that colour pigments.

What may I do for avoid that pigments formation?

I am attempting an extraction of garlic poly sulfides from fresh, aged, or boiled garlic samples suspended in water. (5 grams chopped bulb in 5 ml water) Two pulls with 5 ml chloroform yielded a colorless organic layer but an unchanged yellow aqueous layer. A single pull with hexane gave similar results.

I believe the poly sulfides should be yellow, do they become colorless upon leaving the aqueous phase? If indeed the yellow components are the poly sulfides why are they remaining in the aqueous layer? Is there a better procedure for organic poly sulfide or allicin extraction?

anti cancerous role and main componant of garlic

I am currently working on designing SSR primers for garlic and I'm planning to use EST sequences. I have downloaded ESTs from NCBI's GenBank (though, been trying the garlicdb website but to no luck, I cannot connect to it). Now, my problem is on how to deal with those sequences. I have read several publications on EST-based SSR markers where they directly search for SSRs and then design primers based on the search. While there are also publications where they assemble the ESTs into unigenes or contigs prior to SSR search and primer design.

So, what is the best way to design SSR primers from garlic EST data? Also, could anyone refer a good software to do the contig assembly? I have tried DNA Dragon by Sequentix and, I must say, it is good. However, there is no available citation for the said program. Anyone knows a good FREE program that could assemble contigs? I also have Staden pack (Gap4), but it's quite painful to convert 10k+ of sequences into individual staden files. So, I'm not using it until I am left with no further options.

Thank you in advance!

Can we apply a spectrophotometer?

Is it possible to produce a true garlic seed (onion seed)?

I wish to determine changes in allicin content of peeled garlic cloves during storage

S-allyl cysteine (an organosulfur compound in garlic) separation was carried out with a Ascentis® C18 HPLC Column (5 μm particle size, L × I.D. 25 cm × 4.6 mm, 581325-U, Sigma-Aldrich Co. LLC). Can this column do it?

29-30 degrees south.

We are specifically looking for contacts in Argentina. Recent contact details for genebank curators will be very helpful.

I am looking for analysis methods. Generally, HPLC-UVD is most commonly used, but, peaks are not clear and good. I knew a method, used HPLC-FD with AccQ-Flour Reagent Kit which very expensive for me in VietNam. Can you help and give me a analysis method that simple and cheaper. Thanks!

Hi!

I'm doing a study of garlic SSRs. I'm trying different published SSR markers for garlic and currently optimizing the PCR conditions. I have tried traditional PCR, touch down (TD) and hot start + TD. The result of the TD is the same with the traditional PCR though improved with minimal smearing and more intense bands on the gel (when viewed under UV). However, when I tried hot start + TD the bands varied. The results though are reproducible (tried it 3x) for all cases.

My question now is which one should I carry over? Also, the amp product for HS + TD are larger than the expected product. What can cause the variation in the amplified product bet the TD alone and HS+TD?

I hope someone can help me with this. I have no previous experience on working with SSRs. Any help would be appreciated. TIA!

Hi all,

I'm looking at identifying the organosulphur compounds found in biodiesel made from used cooking oil. One of the main hypotheses is that they originated from cooking sulphur rich foods (onions, garlic etc) so we've got some idea of what we might be looking for. Has anyone got any experience in concentrating organosulphur compounds? e.g. SPE, distillation that we might be able to use to get a richer sample for analysis?

Thanks,

Tom

Is it due to PUFA oxygenation inhibition or anything else?