Questions related to Functional Neuroanatomy
I am trying to get some immunos to work on sections from rat pups aged day 4 – 21. Previously, I perfused pups with 0.9% saline and then 4% PFA, cryopreserved them in 30% sucrose-PFA after PFA fixation and sectioned them on the sliding microtome, getting 50 um sections. The animals perfused well.
However, the sections are extremely fragile, and most of them fail to survive the staining (free floating immunos) procedure. Does anyone have experience with sectioning postnatal rat brains and performing immunohistochemistry on them? Is the fragile nature of these sections normal? If not, what could be causing this? Thanks!
I am pursuing a project that will require me to quantify excitatory synapses in rat spinal cord slices (fixed in PFA). I have a number of references and protocols which describe methods for quantification, but they all use tissue that is 5-15 uM thick.
I would like to use 20 uM thick slices (for a number of other standardized outcome measures), but am concerned that this could be too thick and create too much background/reduce resolution. If you have experience quantifying synapses in fixed tissue, do you believe that this would be an issue?
Is a high basal sympathetic nervous system activity a predictor of short life- span in humans?
I have confocal images of immunostained adult Drosophila brains, and I want to find out more about the neurons that are labelled in my images. I am looking for an online tool where I could ideally select a small brain area and get links to published/database info about the function of specific neurons in that brain area. Could anyone please suggest the best way to do this? Thank you!
I need a file that I can download use for data analysis (basically automatically determining what vascular distribution a stroke has occurred in).
I am currently working on MEG data from patients with GPi and STN DBS. All connectivity studies including ours can find prominent theta connectivity between the temporal lobe and the basal ganglia structures. Theta is the most prominent hippocampal rhythm.
I wish to determine the neuronal density of a part of the brain using an advanced technique. I am hoping that this coupled with other morphological and electrophysiological investigations may help me understand the extent/strength of some particular functions.
MRI data reliability, Intraclass correlation coefficient (ICC), different longitudinal measurements, intra-scanner
Are questions about death, nothingness, world's and life's birth... etc linked to a defined anatomic brain zone (or several)? And in that case, is stimulation of these zones known? Would that contribute to an explain of people's various reactions, when faced with those questions?