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Functional Neuroanatomy - Science topic

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I am trying to get some immunos to work on sections from rat pups aged day 4 – 21. Previously, I perfused pups with 0.9% saline and then 4% PFA, cryopreserved them in 30% sucrose-PFA after PFA fixation and sectioned them on the sliding microtome, getting 50 um sections. The animals perfused well.
However, the sections are extremely fragile, and most of them fail to survive the staining (free floating immunos) procedure. Does anyone have experience with sectioning postnatal rat brains and performing immunohistochemistry on them? Is the fragile nature of these sections normal? If not, what could be causing this? Thanks!
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Sourish Mukhopadhyay did you ever find a solution to this? I ask because I plan to perfuse PD4 rats and perform IHC on free-floating brain tissue, but I am currently doing IHC with PD8 tissue and even at that age I am struggling with the tissue's fragility during staining procedures
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I am pursuing a project that will require me to quantify excitatory synapses in rat spinal cord slices (fixed in PFA). I have a number of references and protocols which describe methods for quantification, but they all use tissue that is 5-15 uM thick. 
I would like to use 20 uM thick slices (for a number of other standardized outcome measures), but am concerned that this could be too thick and create too much background/reduce resolution. If you have experience quantifying synapses in fixed tissue, do you believe that this would be an issue? 
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Hi Khalid,
Look at this paper: 10.1007/s00429-016-1278-x ( i have helped for the quantif and it was ok, as long as you do the deconvolution step!). have also a look to this one: 10.3389/fncir.2013.00153
Hope that helps!
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Is a high basal sympathetic nervous system activity a predictor of short life- span in humans?
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Dear Adejoke,
Hope these papers are useful.
Sympathetic Nervous System and Aging in Man. 
influence of insulin, sympathetic nervous system activity, and obesity on blood pressure: the Normative Aging Study.
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I have confocal images of immunostained adult Drosophila brains, and I want to find out more about the neurons that are labelled in my images. I am looking for an online tool where I could ideally select a small brain area and get links to published/database info about the function of specific neurons in that brain area. Could anyone please suggest the best way to do this? Thank you!
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Virtual Fly Brain were certainly setting up to do something like that a couple of years ago. I've not looked into it too much since, but you can try it out with the link below
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I need a file that I can download use for data analysis (basically automatically determining what vascular distribution a stroke has occurred in).
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You may be interested in these 4 entry points. Contacting these authors should narrow your search.  Be also aware of the variability of the vascular territories.
I am also looking at some similar material.
Ref1.  probabilistic map produced by Michel Dojat in Grenoble france (GIN, Grenoble Institut des Neurosciences INSERM : U836). The digital Atlas of the Blood supply territories of the brain (BST), derived from the 12 printed serial sections in the axial plan developed by Tatu et al. The atlas fits the Talairach space, this 3D atlas is used to determine the stroke subtype
see : Yacine Kabir, Michel Dojat, et al. Multimodal MRI segmentation of ischemic stroke lesions. Conf Proc IEEE Eng Med Biol Soc. 2007 ; 2007: 1595–1598
Ref. 2: the digital probabilistic map of PCA infarcts produced by Thanh G. Phan et al (Digital Map of Posterior Cerebral Artery Infarcts Associated With Posterior Cerebral Artery Trunk and Branch Occlusion - Stroke 2007;38;1805-1811). They register their PCA lesions with the MNI template. It is not a vascular territory map but a map of probability of stroke.
Ref. 3: Another probabilistic map for ICA produced by Jae Sung Lee et al in Seoul.: Probabilistic map of blood flow distribution in the brain from the internal carotid artery. NeuroImage 23 (2004) 1422– 1431.
Ref. 4: Analysis of ischemic stroke MR images by means of brain atlases of anatomy and blood supply territories. W Nowinski et al. Academic Radiology. 13, 8, Pages 1025–1034, August 2006. Their approach is a atlas-to-scan transformation (mapping), quite the opposite of what you may want to do (mapping each scan to the registered atlas).
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I am currently working on MEG data from patients with GPi and STN DBS. All connectivity studies including ours can find prominent theta connectivity between the temporal lobe and the basal ganglia structures. Theta is the most prominent hippocampal rhythm.
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Attached file
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I wish to determine the neuronal density of a part of the brain using an advanced technique. I am hoping that this coupled with other morphological and electrophysiological investigations may help me understand the extent/strength of some particular functions.
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You can try the ImageJ, it's free and you can manage to perform a nice stereological counting.
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MRI data reliability, Intraclass correlation coefficient (ICC), different longitudinal measurements, intra-scanner
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I think that ICC 2 way mixed model, consistency in SPSS may be appropriate... see my article in JRM on my profil page 758;-)
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Are questions about death, nothingness, world's and life's birth... etc linked to a defined anatomic brain zone (or several)? And in that case, is stimulation of these zones known? Would that contribute to an explain of people's various reactions, when faced with those questions?