Fluorescence - Science topic

A R T I C L E I N F O Keywords: Luminescence Thermochromism Silver Cluster Stimuli Temperature A B S T R A C T Stimuli-responsive materials have garnered significant attention in technology, sensors, and biological applications because of their quick response to various external factors. These include light, temperature, change in solvent, pressure...

Random laser gain media are demonstrated by different concentrations of Rhodamine 101 dye solution mixed with SiO 2 nanoparticles. For this purpose, these mediums are fabricated using the sol-gel technique and supplied with a pulsed blue laser to produce random lasing, while the light that reflects is collected using a spectrometer. Fluorescence an...

The indolenine styrylcyanine dye and its carboxyl-labeled derivative were synthesized using condensation of the corresponding indolenines with 4-(dimethylamino) benzaldehyde in the presence of an alkaline catalyst. The spectral-luminescent properties of studied dyes in organic solvents, the aqueous solution (pH 7.9), and the presence of different b...

The present work demonstrates that the twisted intramolecular charge transfer (TICT) and Intramolecular charge transfer (ICT) process can be achieved by structural modification of a molecule. The DMMC (2-(4-(dimethylamino)benzylidene)malononitrile) and DMDC (2-(4-(dimethylamino)benzylidene)malononitrile) exhibit TICT and ICT state respectively via...

Single molecule RNA FISH (smRNA FISH) is an imaging method that labels individual mRNA molecules in cells to facilitate localization and quantitative studies. Here we present a modified protocol for mRNA labelling in Arabidopsis root meristem cells that retains GFP fluorescence. Although the processing steps impact GFP intensity, this protocol demo...

Fluorescence of the vast majority of natural opsin-based photoactive proteins is extremely low, in accordance with their functions that depend on efficient transduction of absorbed light energy. However, several recently proposed classes of engineered rhodopsins with enhanced fluorescence, along with the discovery of a new natural highly fluorescen...

The detection of SO 3 ²⁻ in complex environments and its visualization at the cellular level are critical for understanding its role in biological processes. In this study, we developed an Eu-doped long-wavelength fluorescent carbon quantum dot (CD2) and investigated the detection mechanism, interference effects and cellular imaging applications of...

Detection of toxic hydrazine and harmful strong acidity is of great importance for survival of organisms. In the present paper, a new thiomorpholine substituted malonyl-coumarin dye was synthesized for discriminative detection of hydrazine and strong acidity. At pH 7.4, the fluorescence at 560 nm decreased and that at 496 nm increased upon reaction...

Studies have indicated that intracellular nicotinamide adenine dinucleotide (NAD+) level is associated with the occurrence and development of many diseases. While traditional NAD+ detection techniques are time-consuming and may require large and expensive instruments. We recently found that the CRISPR-Cas12a protein can be inactivated by AcrVA5-med...

This is the first worldwide report of X. fastidiosa (Xf ) subsp. pauca on Castanea sativa and the first characterization of Xf infection on this species. Plants located in three sites (in a long-term affected area in Apulia) were monitored for symptoms and bacterial concentrations in spring and summer, while microscopic analyses were performed to e...

In connection with the increasing anthropogenic load, it is necessary to control the content of various impurities in the atmospheric air. One of these compounds is formaldehyde, which has carcinogenic and mutagenic properties. To measure the concentration of this substance, various methods are used - chromatographic, spectrophotometric, fluorescen...

A Tm³⁺: CaYAlO4 single crystal with disordered structure was prepared using mature Czochralski method. The influence of Tm³⁺ doping on the structure was studied through PXRD measurement and first-principles calculation. The PXRD pattern shows that the introduce of Tm³⁺ does not change the structure of CaYAlO4 matrix. Then, the spectroscopic propert...

Molecules with a π-system that can be mapped onto a Möbius band may display Möbius aromaticity. Such molecules are difficult to synthesize because they have a twisted structure. Recently, we combined chiral [6]helicene and fluorescent [7]cycloparaphenylene and synthesized the first helicene para-phenylene ([6,7]HPP) carbon nanohoop. We have demonst...

Near‐infrared (NIR) fluorophores are highly desirable for super‐resolution fluorescence microscopy; however, their optical properties are typically relatively poor, with low photo‐intensity (mean photon number below 1000), and unfavorably high on‐off blinking duty cycle. In this work, the synthesis and characterization of tetrabenzodinaphthopyranth...

Reactive oxygen species (ROS) are pivotal signaling molecules that control a variety of physiological functions. As a member of the ROS family, peroxynitrite (ONOO−) possesses strong oxidation and nitrification abilities. Abnormally elevated levels of ONOO− can lead to cellular oxidative stress, which may cause several diseases. In this work, based...

Fluorescence confocal microscopy (FCM) represents a novel diagnostic technique able to provide real-time histological images from non-fixed specimens. As a consequence of its recent developments, FCM is gaining growing popularity in urological practice. Nevertheless, evidence is still sparse, and, at the moment, its applications are heterogeneous....

An asymmetric aza-BODIPY analogue bearing quinoxaline moiety was synthesized via a titanium tetrachloride-mediated Schiff-base-forming reaction of 6,7-dimethyl-1,4-dihydroquinoxaline-2,3-dione and benzo[d]thiazol-2-amine. This novel aza-BODIPY analogue forms a complementary hydrogen-bonded dimer due to the quinoxaline moiety in the crystal structur...

By exploiting the nonlinear responses of the fluorescent probes, the spatial resolution of structured illumination microscopy(SIM) can be further increased. However, due to the complex reconstruction process, the traditional reconstruction method of nonlinear structured illumination microscopy (NL-SIM) is relatively slow, which brings a great chall...

Haematococcus pluvialis produces astaxanthin in response to light quality of illumination. To better understand the responses to the combinations of monochromatic red and blue light, the growth, cell differentiation, substance productions, and photosynthetic features defined by the chlorophyll-a (Chl-a) fluorescence techniques were explored. The re...

Image super-resolution techniques exploiting the stochastic fluctuations of image intensities have become a powerful tool in fluorescence microscopy. Compared to other approaches, these techniques can be applied under standard acquisition settings and do not require special microscopes nor fluo-rophores. Most of these approaches can be mathematical...

Fluoride is both necessary and potentially harmful in excessive amounts, making its detection crucial. Fluorescent probes provide a sensitive and selective means for this purpose. In this study, we developed and synthesized a fluorescent probe for LDT using phenothiazine derivatives and aryl vinyl nitrile. Initially non-fluorescent, the probe under...

In the early cerebellar primordium, there are two progenitor zones, the ventricular zone (VZ) residing atop the IVth ventricle and the rhombic lip (RL) at the lateral edges of the developing cerebellum. These zones give rise to the several cell types that form the GABAergic and glutamatergic populations of the adult cerebellum, respectively. Recent...

: The primary objective of this study was to investigate the association of certain genetic alterations and intraoperative fluorescent activity of 5-aminolevulinic acid (ALA) in brain metastasis (BM) of lung adenocarcinoma. A retrospective cohort study was conducted among 72 patients who underwent surgical resection of BM from lung adenocarcinoma a...

Colour centres in wide-bandgap semiconductors feature metastable charge states that can be interconverted with the help of optical excitation at select wavelengths. The distinct fluorescence and spin properties in each of these states have been exploited to show storage of classical information in three dimensions, but the memory capacity of these...

Fabrication techniques for nitrogen-vacancy centres in diamond require the creation of Frenkel defects (vacancy-interstitial pairs) the components of which can interact with formed NV centres affecting their photophysical properties. Here we use Density Functional Theory simulations of inter-defect electronic and strain interactions to explore how...

DNA collected from the environment (eDNA) can provide valuable understanding of ecological patterns and processes. eDNA is highly physically heterogeneous, but this has not been well‐characterized, so most eDNA sampling strategies do not target any particular physical fraction. Consequently, we have limited evidence to understand and interpret the...

The chemosensors act as powerful tool in the detection of metal ions due to their simplicity, high sensitivity, low cost, low detection limit, rapid photophysical response, and application to the environmental and medical felds. This review article presents an overview for the chemosensing of Ag+ ions based on Calix, MOF, Nanoparticle, COF, Calix...

Complexes PyrDPE RuCl and PyrDPE Ruacac with a -extended 2,7-di(4-phenylethynyl)pyrene linker undergo simultaneous one-electron oxidations of their {Ru}-styryl entities. The absence of an intervalence charge-transfer (IVCT) band at intermediate stages,...

An energy audit is an important process for determining a building’s energy efficiency. This process includes verification, monitoring and analysis of energy usage and recommending ways to improve energy consumption. This paper presented a study on energy balance, utility system description, methodology to calculate the energy consumption, load app...

Porphyrin compound-based photochemical molecules and biomaterials have been synthesized for photosensitivity and bioimaging experiments. However, most porphyrin photosensitizers have limited application in biological environments owing to severe aggregation in aqueous solutions. In the present study, we prepared amphipathic and photosensitive copol...

The titer of anti‐severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) neutralizing antibodies (NAbs) in the human body is an essential reference for evaluating the acquired protective immunity and resistance to SARS‐CoV‐2 infection. In this study, a fluorescence‐quenching lateral flow immunoassay (FQ‐LFIA) is established for quantitative d...

In this study, we have prepared several NaLaMgWO6:Dy³⁺, Tb³⁺ phosphors using a high-temperature solid-phase method. X-ray diffraction (XRD) and photoluminescence (PL) were used to analyze the physical phase and luminescent characteristics of these materials. Our results showed that in the NaLaMgWO6:xDy³⁺ phosphor, an ideal doping concentration of x...

Expansion microscopy (ExM) is a highly effective technique for super-resolution fluorescence microscopy that enables imaging of biological samples beyond the diffraction limit with conventional fluorescence microscopes. Despite the development of several enhanced protocols, ExM has not yet demonstrated the ability to achieve the precision of nanosc...

Lithium fluoride (LiF) film detectors for extreme ultraviolet radiation, soft and hard X-rays, based on the photoluminescence of radiation-induced electronic defects, have been proposed and are currently under further development and investigation. LiF film detectors are versatile and can be integrated in different experimental apparatus and imagin...

[This corrects the article DOI: 10.1371/journal.pone.0264554.].

Single crystal fiber (SCF) is a novel solid gain medium and technique which combines the advantages of glass fiber and single crystal, showing great potential in the field of high-power lasers. In this paper, Er, Nd: YSGG single crystals with diameters of 2 mm and lengths of 80 mm were successfully grown using the micro-pulling-down method for the...

Mechanical response luminescence (MRL) describes the photophysical properties triggered by mechanical stimulation. Usually, MRL can be regulated by the intermolecular interaction, molecular conformation or molecular packing, to achieve the desirable...

Purpose: To explore the clinical application value of indocyanine green (ICG)–rituximab in sentinel lymph node biopsy. Methods: This study included 156 patients with primary breast cancer: 50 patients were enrolled in dose-climbing test, and 106 patients were enrolled in verification test. This was to compare the consistency of ICG–rituximab and co...

The fluorescent properties of pomalidomide derivatives have been utilised to develop a high-throughput imaging method suitable for rapid screening of protein degrader candidates.

Background: Early detection of secondary caries near dental restorations is essential to prevent further complications. This systematic review seeks to evaluate the sensitivity of fluorescence and near-infrared (NIR) imaging techniques for detecting secondary caries and to provide insight into their clinical utility. Methods: A comprehensive search...

Structured illumination microscopy (SIM) can double the spatial resolution of a fluorescence microscope and video rate live cell imaging in a two-dimensional format has been demonstrated. However, rapid implementations of 2D SIM typically only cover a narrow slice of the sample immediately at the coverslip, with most of the cellular volume out of r...

Novel quinoline derivatives were synthesized based on 6-amino-substituted quinoline, and antioxidant activity of these compounds is studied by p-nitroso-N,N-dimethylaniline assay. The rate of the reaction between OH radicals and quinoline derivatives is determined by photometric method and the obtained results are compared with that of well-known a...

In this paper, four novel hydrazine fluorescent probes X1–X4 with bis-chalcone structure were designed and synthesized. Through the measurement of its optical properties, it is found that it can quickly identify hydrazine, high sensitivity, low detection limit, and good anti-interference ability. The recognition of hydrazine by probes X1–X4 is not...

Introduction: Ticagrelor is extensively utilized for the treatment of acute coronary syndromes (ACS), but its platelet aggregation inhibitory effects can potentially result in tissue bleeding, posing a serious risk to patients’ lives. Methods: In this study, we developed highly sensitive full length anti-ticagrelor Quenchbodies (Q-bodies) for fast...

Interactions of membrane-resident proteins are important targets for therapeutic interventions but most methods to study them are either costly, laborious or fail to reflect the physiologic interaction of membrane resident proteins in trans. Here we describe highly sensitive cellular biosensors as a tool to study receptor-ligand pairs. They consist...

New 2-(4-(fluorosulfonyloxy)phenyl)benzoxazole (2) was synthesized through the SuFEx click reaction in a two-chamber reactor. The effect of silylation on the yield of the target compound was investigated. The fluorescent properties of compound 2 were determined using experimental and computational methods.

While the identified function of the C-terminus of Atg27p, a transmembrane protein that contributes to cellular self-eating or autophagy, is to control the localization of the protein throughout the endosomal/vacuolar system, the function of its lumenal domain remains unknown. Using bioinformatics tools, we confirm that Atg27 has a predicted mannos...

The assessment of early atherosclerosis (AS) is of great significance for the early diagnosis and mechanism research. Herein, a novel nanoprobe PCN@FL was developed to realize the simultaneous detection and imaging of phosphorylation and hypochlorous acid (HClO). The selective recognition of HClO was achieved through the specific interaction betwee...

The prediction of the metal cluster within a coordination polymer or complex, as well as the dimensionality of the resulting polymer or complex (i.e., 0D, 1D, 2D, or 3D), is often challenging. This is the case for Ph2P(CH2)mPPh2 ligands (1 ≤ m ≤ 8) and CuX salts, particularly for X = I. This work endeavors a systematic statistical analysis combinin...

Fundus angiography, including fundus fluorescein angiography (FFA) and indocyanine green angiography (ICGA), are essential examination tools for visualizing lesions and changes in retinal and choroidal vasculature. However, the interpretation of angiography images is labor-intensive and time-consuming. In response to this, we are organizing the thi...

The vintage of sauce-flavor liquor represents its collection value and grade, so its identification is essential for collectors and connoisseurs. In this research, a vintage identification model for sauce-flavor liquors was proposed based on the fluorescence spectroscopy method. A fluorescence spectrophotometer was used to measure the fluorescence...

Optical imaging in scattering media is important to many fields but remains challenging. Recent methods have focused on imaging through thin scattering layers or thicker scattering media with prior knowledge of the sample, but this still limits practical applications. Here, we report an imaging method named ‘speckle kinetography’ that enables high-...

The steady-state characteristic parameters of the loads are used to identify new energy grid-connected loads using an event-based network analysis method in this paper. The analysis of interdependence among elements is done by studying the element layer and special structure of the network analysis method. Using the principle of limit relative rank...

We aimed to discover new tamoxifen derivatives with antimicrobial potential, particularly targeting colistin-resistant Acinetobacter baumannii. We determined the MIC of 22 tamoxifen derivatives against both A. baumannii wild-type and colistin-resistant strains using microdilution assays, in monotherapy and in combination with colistin. The antibact...

Four molecules bearing carbazole ( Cz ), phenoxazine ( PXZ ) and phenothiazine ( PTZ ) as donor groups to diphenylsulfone acceptor have been reported. Molecule containing Cz just shows thermally activated delayed fluorescence (TADF) property, but molecules containing PXZ and PTZ display TADF and aggregation-induced emission (AIE) properties. In thi...

Using a unified metal-free procedure, a selection of Thermally Activated Delayed Fluorescence (TADF) emitters has been synthesized and characterized. Different acceptor and donor moieties have been explored in order to develop red emitting dyes with reduction potentials suitable for the application in ECL using tri-propylamine as coreactant. The mo...

Silver ions (Ag+) exist widely in various areas of human life, and the food contamination caused by them poses a serious threat to human health. Among the numerous methods used for the detection of Ag+, fluorescence and colorimetric analysis have attracted much attention due to their inherent advantages, such as high sensitivity, simple operation,...

Hypochlorite (ClO–), a crucial chemical in the living organism, engages in various physiological activities. However, high amounts of ClO– result in oxidative damage. In this work, a commercially available 2–aminoanthracene (AA) was used to detect ClO–. AA demonstrated distinct properties such as superior selectivity and rapid response (< 30 s) wit...