Science method
Flow Cytometry - Science method
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Publications related to Flow Cytometry (10,000)
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Background: Embryonic antigens (EA) regulate pluripotency, self-renewal, and differentiation in embryonic stem (ES) cells during their development. In adult somatic cells, EA expression is normally inhibited; however, EAs can be re-expressed by cancer cells and are involved in the deregulation of different signaling pathways (SPs). In the context o...
Variants of uncertain significance (VUS) in CTLA4 are frequently identified in patients with antibody deficiency or immune dysregulation syndromes including, but not limited to, patients with multi-organ autoimmunity and autoinflammation. However, to ascertain the diagnosis of CTLA4 insufficiency, the functional relevance of each variant needs to b...
Th9 cells play a crucial role in parasite immunity. The development of Th9 cells is facilitated by several cytokines. Key transcription factors, such as STAT6, STAT5, and PU.1, are known to enhance IL-9 expression during the Th9 immune response. NF-κB-mediated transduction pathways participate in the induction of IL-9. In a previous study, we unvei...
Introduction
The three groups of helper innate lymphoid cells (ILCs), namely ILC1, ILC2 and ILC3, have been identified by flow cytometry by combinations of cell surface markers. Here, we review various ways ILCs are currently identified, focusing on potential problems and their solutions. The first step to identify all ILCs is to exclude other lymp...
Heparin-induced thrombocytopenia (HIT) is a life-threatening complication of heparin therapy (both unfractionated heparin and low-molecular-weight heparin). In our study, we examined a group of 122 patients with suspected HIT. The samples of all patients were analyzed in the first step using an immunoassay (ID-PaGIA Heparin/PF4, Hemos1L-Acustar HIT...
Objective
Multiple myeloma (MM) is a highly characteristic tumor that is influenced by numerous factors that determine its prognosis. Studies indicate that the presence of circulating plasma cells (cPCs) is a detrimental factor that significantly impacts the prognosis of patients with MM.
Methods
This study retrospectively analyzed the prognostic...
Tumor recognition by T cells is essential for antitumor immunity. A comprehensive characterization of T cell diversity may be key to understanding the success of immunomodulatory drugs and failure of PD-1 blockade in tumors such as multiple myeloma (MM). Here, we use single-cell RNA and T cell receptor sequencing to characterize bone marrow T cells...
Time-domain measurement of the fluorescence lifetime in flow cytometry offers a complementary method to traditional flow cytometry, especially for the analysis of cells and biomolecules. Short measurement times due to high throughputs (cells/s) make a suited detector mandatory for a sufficient detection of the fluorescence signal and therefore the...
Cancer is the major cause of death worldwide, and the anticancer effect of ginseng and its main root has been studied. However, study of fine root of ginseng (FRG) is still insufficient. The purpose of this study was to discover a new anticancer effect from FRG, which does not show an anticancer effect, through a bioconversion technique. We measure...
Eastern gamagrass (EG), Tripsacum dactyloides , is a diverse warm season, perennial bunchgrass that is widespread in the eastern half of the United States. Study objectives were to obtain a large assortment of ecotypes, measure and quantify some of their morphological aspects, and determine ploidy level for many of these ecotypes. Nearly 300 ecotyp...
Kikuchi–Fujimoto disease (KFD) is a rare, benign lymphoproliferative disease of uncertain origin that can mimic other inflammatory or clonal lymphoproliferative disorders. Given the lack of available blood biomarkers, diagnosis is based on the biopsy of an affected lymph node. In recent years, evidence has been mounting that a dysregulated type I I...
FACS
can be used to sort live cells based on similar principles. It allows to unravel the mysteries hidden
within the diverse populations of cells that make up living organisms. Protein specific fluorescent dyes
are used to stain the cells for analysis and automatic sorting according to the characteristic wavelength
of light scattered by them.
In this study, it was aimed to investigate the effects of melamine exposure since the weaning period on ovarian tissue and ovarian reserve. Melamine is illegally added to milk and formula to provide high false protein positivity. Female rats (the weaning period = 21 days old, n = 18) were divided into 3 groups. 0.1 mL saline was applied to the cont...
High-speed image processing is essential for many real-time applications. On-chip photonic neural network processors have the potential to speed up image processing, but their scalability is limited in terms of the number of input/output channels because high-density integration is challenging. Here, we propose a photonic time-domain image processi...
The relative contribution of small (sEVs) and large extracellular vesicles (lEVs) to the total plasma procoagulant potential is not yet well defined. Thus, we compared total and TFpos-sEVs and -lEVs isolated from healthy subjects and COVID-19 patients during the acute phase of the infection and after symptom remission in terms of (1) vesicle enumer...
Abstract Background There are many studies on the relationship between vitamin D and coronavirus disease 2019 (COVID-19), while the results are matters of debate and the mechanisms remain unknown. The present study was performed to assess the impact of serum 25-hydroxyvitamin D [25(OH)D] levels on the severity of disease in hospitalized COVID-19 pa...
Cell subtype identification from mass cytometry data presents a persisting challenge, particularly when dealing with millions of cells. Current solutions are consistently under development, however, their accuracy and sensitivity remain limited, particularly in rare cell-type detection due to frequent downsampling. Additionally, they often lack the...
Given the paucity of data surrounding the prognostic relevance of circulating lymphoma (CL) in Waldenström macroglobulinemia (WM), we sought to evaluate the impact of CL at diagnosis on outcomes in patients with WM. Patients were divided into CL+ and CL- based on the results of flow cytometry. The endpoints included assessing progression-free survi...
Pleural effusion is a rare complication of lymphoplasmacytic lymphoma. When it occurs, traditional investigation techniques may not always be successful. The flow cytometry is a fast and precise diagnostic technique, which can be helpful in the diagnosis of pleural localization of hematological diseases. We present a case report of a pleural locali...
Identifying cell populations in flow cytometry data is mostly done via a “manual gating” method that often lacks verifiability and reproducibility, even in the hands of experienced investigators. In 2018, flowLearn outperformed the previous best automatic gating methods by demonstrating near perfect identification of known populations on two fluore...
Tissue-resident macrophage populations constitute a mosaic of phenotypes, yet how their metabolic states link to the range of phenotypes and functions in vivo is still poorly defined. Here, using high-dimensional spectral flow cytometry, we observe distinct metabolic profiles between different organs and functionally link acetyl CoA carboxylase act...
T-cell acute lymphoblastic leukemia (T-ALL) represents an area of highly unmet medical needs. Once relapsed, patients have limited treatment options and poor prognosis. T-ALL antigens such as CD7 is extensively expressed in normal T cells and natural killer (NK) cells, and extending the success of CAR-T therapy to T cell malignancies was challenged...
We report the case of a 75-year-old female presented with lethargy, Hb 93 g/L, WBC 64 x 109/L, platelet 110 x 109/L. Blood film showed blasts, myelocytes, metamyelocytes, neutrophils. Quantitative PCR detected p210 BCR::ABL1 transcript in sorted CD19+ cells, and sorted CD19- cells. Bone marrow smear was packed with blasts. Flow cytometry and bone m...
The conjugation of a fluorophore and a variety of cell-penetrating peptides onto a RAFT agent allowed for the synthesis of polymers of defined sizes with quantifiable cell-uptake. Each peptide-RAFT agent was used to polymerize acrylamide, acrylate, and styrene monomers to form high or low molecular weight polymers (here 50 or 7.5 kDa) with the pept...
There is still no study investigating the prognostic performance of CD14⁺⁺CD16⁻, CD14⁺⁺CD16⁺ and CD14⁺CD16⁺⁺ monocyte subpopulations in complicated intra-abdominal infections (cIAIs); therefore, we aimed to evaluate the association between monocyte subtypes and outcome in such patients. A single-center prospective study was conducted at a Universit...
Endometriosis is a common gynecological disease which is characterized by endometriotic lesions outside the uterine cavity. In this study, we investigated whether the presence of pre-existing endometriotic lesions promotes the development of new lesions due to the exchange of cells and an altered peritoneal environment. For this purpose, uterine ti...
Cell communication orchestrates development, homeostasis, and disease. Understanding these processes depends on effective methods to study how cells influence their neighbours. Here, we present Positive Ultra-bright Fluorescent Fusion For Identifying Neighbours (PUFFFIN), a novel cell neighbour-labelling system based upon secretion and uptake of a...
Knowledge about early immunity to SARS‐CoV‐2 variants of concern mainly comes from analysis of human blood. Such data provide limited information about host responses at the site of infection and largely miss initial events. To gain insights into compartmentalization and early dynamics of host responses to different SARS‐CoV‐2 variants, we utilized...
Staphylococcus aureus (S. aureus) is the leading bacterial cause of death in high-income countries and can cause invasive infections at various body sites. These infections are associated with prolonged hospital stays, a large economic burden, considerable treatment failure, and mortality rates. So far, there is only limited knowledge about the spe...
Introduction
Stem cell laboratories measure the number of viable CD34+ and CD45+ cells in their products by flow cytometry following a standard protocol (ISHAGE). Although the ISHAGE protocol is well documented, its correct application can be challenging. Automated gating algorithms have been created to address this issue, but they have limitations...
Introducción: El elevado costo de la citometría de flujo para el seguimiento de los valores de CD4+ en pacientes con VIH ha justificado la búsqueda de metodologías predictivas de este valor, que contribuyan a simplificar y reducir los costos del seguimiento terapéutico de los pacientes con el VIH/SIDA. Objetivo: establecer las posibles corresponden...
Flow cytometry (FCM) can investigate dozens of parameters from millions of cells and hundreds of specimens in a short time and at a reasonable cost, but the amount of data that is generated is considerable. Computational approaches are useful to identify novel subpopulations and molecular biomarkers, but generally require deep expertize in bioinfor...
Introduction
Umbilical cord blood (UCB) is an important source of hematopoietic stem cells for transplantation in some hematological diseases. One of the main factors influencing sample quality and transplantation success, in addition to histocompatibility, is the number of viable CD34+ cells. Over the years, different factors that could directly a...
Acute megakaryoblastic leukaemia (AMkL) is a rare subtype of acute myeloid leukaemia (AML) representing 5% of all reported cases, and frequently diagnosed in children with Down syndrome. Patients diagnosed with AMkL have low overall survival and have poor outcome to treatment, thus novel therapies such as CAR T cell therapy could represent an alter...
Aim: Our aim is to build a precise automatic tool for the diagnosis of CLL with the help of machine learning algorithms and flow cytometry immunophenotypic data. Methods: We run experiments with two machine learning methods. First one is decision tree which was previously used in other similar works and second one is support vector machines which i...
Objective: This study analysed the frequencies of clinical and laboratory risk factors and cytogenetic abnormalities in Jordanian children with acute lymphoblastic leukaemia (ALL) and their response to therapy. Methods: We conducted a retrospective analysis of 116 paediatric ALL cases diagnosed and treated in Queen Rania Hospital between 2015 and 2...
Research on various species has revealed a connection between genome size variation and the physiological and ecological characteristics of the species, suggesting that it could be a crucial factor influencing a species’ adaptability to different environments. Wheat, being one of the world’s three primary grains, holds significance in this regard....
Combination treatment is the most effective therapy strategy on cancer by dramatically reducing drug toxicity and enhancing efficacy. In this study, we assessed whether temsirolimus, an mTOR inhibitor enhanced anti-cancer effects of cisplatin on human cervical Hela cell line or not. The combination of temsirolimus and cisplatin, to a large extent,...
Background:
The incidence of ovarian cancer ranks third among gynecologic malignancies, but the mortality rate ranks first.
Methods:
The expression of GAS5 is low in ovarian cancer and is associated with the low survival of ovarian cancer patients according to public ovarian cancer databases. GAS5 overexpression inhibited ovarian malignancy by a...
Evidence comparing ultrasound endoscopy-guided fine-needle biopsy (EUS-FNB) with EUS-guided fine-needle aspiration (EUS-FNA) in deep-seated lymphoma tissue sampling is insufficient. This study aims to evaluate the diagnostic efficacy of immunohistochemistry (IHC) or flow cytometry (FCM) on specimens obtained from EUS-FNB and EUS-FNA in the diagnosi...
New techniques are revolutionizing single-cell research, allowing us to study microbes at unprecedented scales and in unparalleled depth. This review highlights the state-of-the-art technologies in single-cell analysis in microbial ecology applications, with particular attention to both optical tools, i.e., specialized use of flow cytometry and Ram...
We previously developed a highly sensitive and specific anti-PDPN mAb, LpMab-23 (mouse IgG1, kappa). In this study, we produced a humanized IgG1 version (humLpMab-23) and its defucosylated form (humLpMab-23-f) of an anti‑PDPN mAb to potentiate the ADCC activity. The humLpMab-23 could recognize PDPN‑overexpressed Chinese hamster ovary (CHO)-K1 (CHO/...
Human γδ T cells are highly enriched in epithelial cell-dominated compartments like skin. Nonetheless, their function in the pathogenesis of pemphigus vulgaris (PV), an autoimmune skin disorder, is lacking. Therefore, we investigated the functional expression of human γδT cell subsets along with their homing chemokine receptor-ligand and inflammato...
Purpose:
Endothelial injury, involved in the pathogenesis of renal fibrosis, can generate microparticles (MPs). These are 0.1-1 µm membrane-bound vesicles shed from the damaged or activated cell surfaces. We analyzed the presence of circulating MPs and EnMPs in IgAN and correlated with markers of endothelial injury and disease activity.
Methods:...
Immunological aging type definition requires establishing reference intervals from the distribution of immunosenescence biomarkers conditional on age. For 1605 individuals (18–97 years), we determined the comprehensive immune age index IMMAX from flow-cytometry-based blood cell sub-populations and identified age-specific centiles by fitting general...
Measurable residual disease (MRD) is an important biomarker in acute myeloid leukemia (AML). However, MRD cannot be detected in many patients using current methods. We developed a highly sensitive 5-hydroxymethylcytosine (5hmC) signature in cell-free DNA by analyzing 115 AML patients and 86 controls. The 5hmC method detected MRD in 20 of 29 patient...
Men who have sex with men (MSM) with HIV are at high risk for squamous intraepithelial lesion (SIL) and anal cancer. The identification of local immunological mechanisms involved in the development of anal dysplasia could aid treatment and diagnostics. We performed a study of 111 anal biopsies obtained from 101 MSM with HIV, who participated in an...
Purposes: There is often vibration in red blood cells (RBCs) processing, blood transfusion, blood conveyance and so on. The effect of vibration on human RBCs should be researched for more details in order to reducing the deviation in experiments with vibration of device.
Methods: In this paper, flow cytometry is applied to detect the deformation, m...
Flow cytometry estimates of genome sizes among species of Drosophila show a 3-fold variation, ranging from ∼127 Mb in Drosophila mercatorum to ∼400 Mb in Drosophila cyrtoloma. However, the assembled portion of the Muller F Element (orthologous to the fourth chromosome in Drosophila melanogaster) shows a nearly 14-fold variation in size, ranging fro...
Knowledge of blood typing is necessary because receiving incompatible blood during a transfusion can result in severe and potentially lethal transfusion responses. The properties of blood are vital for clinical applications, and numerous blood tests are frequently conducted in medical settings to diagnose and monitor a wide range of medical disorde...
Numerical simulations have shown that finescale structures such as fronts are often suitable places for the generation of vertical velocities, transporting subsurface nutrients to the euphotic zone and thus modulating phytoplankton abundance and community structure. In these structures, direct in situ estimations of the phytoplankton growth rates a...
Background: Pseudomonas aeruginosa (Pa) causes nosocomial infections, it has the ability to form biofilms in medical devices leading treatment to complicated. The purpose of this study is to build a biofilm model on Drip Flow Reaction (DFR) system and apply it to investigate the effects of antibiotics on biofilm formation.
Materials and method: Th...
Purpose
Inflammatory bowel disease (IBD) occurring following allogenic stem cell transplantation (aSCT) is a very rare condition. The underlying pathogenesis is poorly defined. There is currently no systematic effort to exclude loss- or gain-of-function mutations in immune-related genes in stemcell donors.
Methods
Whole exome sequencing of hematop...
Antecedentes: La deficiencia especifica de anticuerpos (SAD) es un error innato de la inmunidad, en pacientes de más de 2 años, caracterizada por niveles de inmunoglobulinas y subclases de IgG normales, pero con infecciones recurrentes y respuestas de anticuerpos disminuidas a antígenos polisacáridos. Reporte de caso: Femenina de 10 años, previa sa...
Myeloid cells, specifically microglia and macrophages, are activated in retinal diseases and can improve or worsen retinopathy outcomes based on their inflammatory phenotype. However, assessing the myeloid cell response after retinal injury in mice remains challenging due to the small tissue size and the challenges of distinguishing microglia from...
This protocol describes the Intracellular Staining (ICS) Senescence Flow Cytometry Panel
This protocol describes the T/NK Immunosenescence Profiling Flow Cytometry Panel
Waldenström Macroglobulinemia (WM) is a lymphoplasmacytic lymphoma with bone marrow (BM) involvement and IgM monoclonal gammopathy. To date, no studies have focused specifically on peripheral blood (PB) involvement. In this study, 100 patients diagnosed with WM according to the World Health Organization (WHO) criteria were included based on the dem...
Breast cancer is the most common form of malignancy among women worldwide and is a multifactorial disease in the development of which various factors are involved. Faradarmani Consciousness Field (FCF) was introduced by Mohammad Ali Taheri as one of many Consciousness Fields that are neither energy nor matter, nor are they quantifiable, thus they c...
VTEA is a tool for 3D tissue cytometry (3DTC) of large 3D fluorescence light microscopy image volumes ranging from 50-100s of microns thick. The objective was to unify image processing, segmentation, data exploration and analysis in one package. VTEA is a tool that is… …free and easy to get.It utilizes ImageJ/Fiji as the distribution platform. . .....
Background
This project aimed to research the significance of THRIL in the diagnosis of benign and malignant solitary pulmonary nodules (SPNs) and to investigate the role of THRIL/miR-99a in malignant SPNs.
Methods
The study groups consisted of 169 patients with SPN and 74 healthy subjects. The differences in THRIL levels were compared between the...
B cells are key pathogenic drivers of chronic inflammation in rheumatoid arthritis (RA). There is limited understanding of the relationship between synovial B cell subsets and pathogenic antibody secreting cells (ASCs). This knowledge is crucial for the development of more targeted B-cell depleting therapies. While CD11c+ double-negative 2 (DN2) B...
There remains a lack of consensus as to the most appropriate primary therapy in Waldenstrőm macroglobulinemia (WM). We evaluated a novel bortezomib-based combination and developed a sensitive WM-specific flow cytometry assay (limit of detection 0.004% of leucocytes) to assess bone marrow (BM) response. Sixty treatment-naïve WM patients were enrolle...