Science method
Flow Cytometry - Science method
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Publications related to Flow Cytometry (10,000)
Sorted by most recent
Among the hematopoietic neoplasms, leukemias are caused by myeloid or lymphoid cells in the bone marrow, which can either be acute with an unfavorable prognosis and mostly affect cats that tested positive for feline viral leukemia (FeLV) or chronic in older cats that have a poor prognosis. Leukemias have several classifications in which the differe...
Immune thrombocytopenic purpura (ITP) is a disease with a pathogenesis that remains unclear. Accordingly, this study aims to explore the role of B10 cells in ITP pathogenesis and provide a potential novel target for ITP treatment. We selected 42 children diagnosed with ITP and 29 age- and sex-matched healthy children as study objects. Peripheral bl...
Background/Objectives: The treatment of choice for prostate cancer is androgen deprivation (ADT) and novel hormonal agents such as abiraterone, Enzalutamide or Apalutamide. Initially, this therapy is highly effective, but a significant challenge arises as most patients eventually develop resistance, resulting in castration-resistant prostate cancer...
Background
Pancreatic cancer (PC) is a major contributor to global cancer‐related mortality. While the inhibitory effect of metformin (Met) on PC has been reported, the underlying mechanism remains elusive.
Methods
We established BxPC‐3 cell models with miR‐378a‐3p and VEGFA knockdown. The expression of miR‐378a‐3p, VEGFA, and RGC‐32 in PC and nor...
Background/Objectives: In a previous study, we described elevated anti-Anisakis IgG levels in septic patients in relation to disease severity. In this study, our objective was to analyze the evolution of anti-Anisakis immunoglobulins in septic patients during hospital admission and their association with αβ and γδ T cell subsets. Methods: We recrui...
It is well known that the suboptimal thawing of frozen insemination doses critically reduces the fertilizing ability of spermatozoa and, consequently, the effectiveness of artificial insemination. In this study, we aimed to determine the optimal thawing procedure for frozen Wallachian ram insemination doses. The study used a standard method of obta...
Anoectochilus spp.) là một trong những cây dược liệu quan trọng được dùng để chữa bệnh như huyết áp, tiểu đường, tim mạch, gan, phổi cũng như phục hồi sức khỏe. Cảm ứng đa bội bằng colchicine và oryzalin được ứng dụng rộng rãi trên nhiều loài thực vật và được xem là công cụ hữu hiệu trong chọn tạo giống cây trồng. Trong nghiên cứu này, chồi non lan...
This paper evaluates various deep learning methods for measurable residual disease (MRD) detection in flow cytometry (FCM) data, addressing questions regarding the benefits of modeling long-range dependencies, methods of obtaining global information, and the importance of learning local features. Based on our findings, we propose two adaptations to...
Background
Pilon fractures are challenging to treat and carry a risk of delayed healing. MicroRNA (miRNA) is closely associated with various diseases due to its ability to regulate gene expression. Consequently, this study aimed to examine the connection between miR-1271-5p expression levels and pilon fracture healing processes, while also explorin...
![Figure 11: OMIP-047[4] Emulation of the Published Gate Sequence.](publication/386053157/figure/fig4/AS:11431281292057008@1732341890903/OMIP-0474-Emulation-of-the-Published-Gate-Sequence_Q320.jpg)
One of the most common objectives in the analysis of flow cytometry data is the identification and delineation of phenotypes, distinct populations of cells with shared characteristics in the measurement dimensions. We have developed an automated tool to comprehensively identify these cell populations by Exhaustive Projection Pursuit (EPP). The meth...
Certain molecules found on the surface or within the cargo of extracellular vesicles (EVs) are linked to osteoarthritis (OA) severity and progression. We aimed to identify plasma pathogenic EV subpopulations that can predict knee radiographic OA (rOA) progression. We analyzed the mass spectrometry-based proteomic data of plasma EVs and synovial flu...
Monoclonal gammopathy of undetermined significance (MGUS) is the precursor of multiple myeloma (MM) and related disorders. MGUS is characterized by asymptomatic paraproteinemia. In some cases, multiple paraproteins can be identified but the clinical implications of this phenomenon are poorly understood. In this study, we aim to inform the approach...
Objective
Many investigation have sought to identify therapeutic targets and treatment strategies for skin cutaneous melanoma (SKCM). Numb, an endocytic adaptor protein, is known to act as a tumor suppressor in various human cancers. However, the roles of Numb and its homolog NumbL in immune microenvironment, and their effect on melanoma remain lar...
About 60 samples were collected from patients with varicella zoster, including 24 males and 36 females, as well as from healthy people, which included 29 samples (15 males and 14 females), aged between 15 and 60 years. Data were collected in the holy city of Najaf between November, 2022 and July, 2023. VZV patients were selected from Sadr Medical C...
Background
Treatment of Helicobacter pylori (H. pylori) infections is challenged by antibiotic resistance. The urease enzyme contributes to H. pylori colonization in the gastric acidic environment by producing a neutral microenvironment. We hypothesized that urease inhibition could affect H. pylori viability. This work aimed to assess the effects o...
Manual expert gating remains common practice for the definition of specific cell populations in the analysis of flow cytometry data. The increasing number of measured parameters per individual cell and high inter-rater variability makes manual gating inconsistent in many scenarios such as multi-center studies. Here, we propose ConvexGating, an AI t...
Background
In Ethiopia, cutaneous leishmaniasis is mainly caused by Leishmania (L.) aethiopica parasites and presents in three main clinical forms. It is still not clear if the host immune response plays a role in the development of these different presentations. Since neutrophils are likely to be one of the first immune cells present at the site o...
Leukocytes, or white blood cells, play a critical role in the body's immune defense system. The classification of leukocytes is essential for diagnosing various hematological disorders, including leukemia. Leukemia, a group of cancers characterized by the uncontrolled proliferation of abnormal white blood cells, requires early and accurate detectio...
Background
In acute myeloid leukemia (AML), further investigation is warranted to integrate measurable residual disease (MRD) with genetic characteristics for formulating a dynamic prognostic system for predicting response and selecting appropriate postremission therapeutic strategies.
Methods
The authors incorporated MRD with genetic risk classif...
Background
The objective was to evaluate the level of neutrophil extracellular traps (NETs) in patients with chronic kidney disease (CKD) and periodontitis, and to explore the relationship between NETs and both diseases.
Methods
63 CKD and 40 non-CKD participants were recruited and underwent periodontal examination, among which 35 early CKD patien...
Chronic lymphoproliferative disorder (CLPD) represents a heterogenous group of conditions affecting lymphocytes – especially white blood cells crucial for combating infections. The utilization of flow cytometry for immunophenotyping has significantly improved the diagnosis and differentiation between various CLPDs. The diagnosis of CLPD is based on...
Background. While ultrasound-guided fine-needle aspiration cell block (FNACB) is a cost-effective, expeditious, and reliable procedure used routinely in the initial evaluation of head and neck masses, it has limited efficacy in diagnosing lymphoproliferative disorders such as non-Hodgkin lymphoma (NHL). Flow cytometry performed on an fine-needle as...
In response to the urgent need for new anti-proliferative agents, four novel series of triazolopyrimidine compounds (7a–e, 9a–d, 11a–f, and 13a–e) were synthesized and evaluated for in vitro anticancer efficacy against HCT116, HeLa, and MCF-7 cell lines. Compound 13c emerged as the most potent, with IC50 values of 6.10, 10.33, and 2.42 μM respectiv...
Euphorbia hirta has anti-inflammatory effects in traditional medicine, but its anti-inflammatory mechanism has not been explored at the cellular and molecular levels. To unravel these mechanisms, the main active components in the 65 and 95% ethanol extracts of Euphorbia hirta were first identified by UPLC-Q-TOF/MS. Subsequently, potential anti-infl...
Background
The use of CRISPR/Cas9-gene-editing to enhance the anti-tumor activity of T cell therapies is a promising approach in the treatment of patients with solid tumors. We previously reported the utility of in vivo pair-wise CRISPR/Cas9 screens to identify the best target combinations in T cells able to enhance anti-tumor function, with SOCS1...
Background: O-6-methylguanine-DNA methyltransferase is responsible for the direct repair of O6-methylguanine lesions induced by alkylating agents, including temozolomide. O-6-methylguanine-DNA methyltransferase promoter hypermethylation is a well-established biomarker for temozolomide response in glioblastoma patients, also correlated with therapeu...
Introduction
This study aimed to evaluate the prognostic value of the Mayo additive staging system (MASS) and minimal residual disease (MRD) in newly diagnosed multiple myeloma (NDMM) patients.
Methods
A total of 238 NDMM patients were enrolled in Beijing Chaoyang Hospital. Fluorescence in‐situ hybridization and next‐generation flow cytometry were...
Understanding how nanoparticle properties influence uptake by cells is highly important for developing nanomedicine design principles. For this, quantitative studies where actual numbers of cell-associated particles are determined are highly relevant. However, many techniques able to measure particle numbers suffer from low-throughput or place requ...
A new hexaploid cytotype of Aegilops crassa has been identified in Türkiye. To assess the ploidy levels of native populations, 50 samples from Adıyaman, Batman, Bitlis, Diyarbakır, Hakkari, Mardin, Siirt, Şanlıurfa, Şırnak, and Van were analyzed using flow cytometry and cytogenetic techniques. DNA content was determined by comparison with standard...
Purpose
The present study explores the dynamics of cell death in Hirschsprung’s disease (HSCR) and control (CO) groups under inflammatory stress conditions.
Methods
Using flow cytometry, we analyzed intestinal colonic organoid cultures derived from the ganglionic segment of the HSCR and CO groups. Our analysis focused on the quantification of RIPK...
Minimal residual disease (MRD) is of growing interest in light chain (AL) amyloidosis and is associated with higher rates of cardiac response. A new graded cardiac response criteria has been proposed for better assessment of cardiac improvement. We evaluated MRD status in 63 patients with cardiac AL amyloidosis using next generation flow cytometry...
To study the changes of lymphocyte subsets in children with acute lymphoblastic leukemia (ALL) at initial diagnosis and compare them with those of healthy children and patients who have achieved complete remission (CR). Furthermore, we aim to analyze the prognostic significance of lymphocyte subsets in these patients. From May 2011 to December 2015...
Acute lymphoblastic leukemia (ALL) is a hematopoietic disorder that mainly affects the child population, and it is characterized by the presence of lymphoid progenitor or precursor cells with different genetic alterations. The origin of this disease is controversial, since some authors assumed that leukemic transformation occurs in a lymphoid proge...
Leishmaniasis is recognized as a serious public health problem in Brazil and around the world. The limited availability of drugs for treatment, added to the diversity of side effects and the emergence of resistant strains, shows the importance of research focused on the development of new molecules, thus contributing to treatments. Therefore, this...
Induced pluripotent stem cells (iPSCs) are a promising cell source because of their pluripotency and self-renewal abilities. However, there is a risk of pluripotency loss during cell expansion. Particularly, cell passaging is associated with a higher risk of decreasing cell quality. There are two iPSC passaging methods: single-cell and clump passag...
Cisplatin-based chemotherapy serves as the standard of care for individuals with advanced stages of gastric cancer. Nevertheless, the emergence of chemoresistance in GC has detrimental impacts on prognosis, yet the underlying mechanisms governing this phenomenon remain elusive. Level of mitophagy and ferroptosis of GC cells were detected by fluores...
CD34⁺ progenitor cells with angiogenic capabilities traffic into blood during exercise and extravasate afterwards but the magnitude of this response varies between people. We examined whether exercise‐induced progenitor cell trafficking is influenced by cardiorespiratory fitness (maximum oxygen uptake; V̇O2max${{\dot{V}}_{{{{\mathrm{O}}}_2}{\mathrm...
Introduction
Single-cell RNA sequencing (scRNAseq) and flow cytometry studies in skin are methodologically complex and costly, limiting their accessibility to researchers worldwide. Ideally, RNA and protein-based analyses should be performed on the same lesion to obtain more comprehensive data. However, current protocols generally focus on either s...
Purpose : This study aimed to investigate the correlation of the circulating T lymphocytes with response to neoadjuvant chemotherapy combined immune-oncology therapy (neoCTIO) in operable esophageal squamous cell carcinoma (ESCC) and explore the predictive markers. Methods : ESCC patients staged cT2N1-2M0 or cT3-4aN0-2M0 were enrolled. All patients...
Previous evidence suggested that B lymphocyte may involve in the progression and prognosis of multiple myeloma (MM). However, the prognostic value of peripheral B lymphocyte counts on MM before and after treatment in the novel agent era was rare reported. Herein, we conducted a retrospective study in our center to detect peripheral B lymphocyte cou...
Chronic heat stress induced by global warming has important implications for fish survival, and the response of important immune organs, such as the spleen, to chronic heat stress remains to be investigated. In this study, one control group (20 ℃) and two heat stress groups (24 ℃ and 28 ℃) were set up to investigate the effects of heat stress on th...
Background: Detailed characterization of B cells in dialysis patients who are candidates for kidney transplant is still lacking, with little information on how dialysis duration and modality impact B cell subsets. Methods: Cluster analysis of flow cytometry determined the frequencies and absolute numbers of B-cell subsets and divided the cohort of...
Platelets play a crucial role in blood transfusions, and understanding the changes that occur during their storage is important for maintaining the quality of preparations. In this study, we examined key alternating factors, with a particular focus on platelet activation and the release of extracellular vesicles. Additionally, we compared two detec...
Objective
Current therapies targeting individual factors in inflammatory arthritis show variable efficacy, often requiring treatment with combinations of drugs, and are associated with undesirable side effects. NF‐ĸB is critical for the production and function of most inflammatory cytokines. However, given its essential role in physiologic processe...
99 de sangrados en pacientes que no tenían otra opción que el tra-tamiento episódico a demanda. Además, esta terapéutica ha de-mostrado ser segura en el escenario socio-sanitario estudiado. Por otra parte, se pretende ampliar el estudio genético a todos los pacientes (actuales y futuros) y analizar la potencial relevancia de la base molecular causa...
Skeletal muscle is critically dependent on the function of muscle stem cells (MuSCs) for effective muscle repair following injury. Here, we detail a protocol for the isolation of primary muscle cells and subsequent analysis of proliferation capacity in vitro using EdU (5-ethynyl-2′-deoxyuridine) on fixed cells. We also describe a cell death analysi...
- [...]
Extracellular vesicles (EVs) are vesicular structures comprised of a bilayer lipid membrane, released by living cells. There is a growing body of evidence for their functionality, indicating that small EVs (sEVs) can mediate specific forms of intercellular communication. The future applications of sEVs hold great promise, not only as diagnostic mar...
This study aims to analyze the clinical features, Radiological findings and diagnostic characteristics (tissue biopsy, flow cytometry and cerebrospinal fluid cytology) of primary central nervous system lymphoma (PCNSL). To improve the diagnosis of the disease.
A total of 19 patients with primary central nervous system lymphoma admitted to the Secon...
Aim. For the first time, flow cytometry has been used to study the structure and functional characteristics of bacterio‐, pico‐ and nano‐ phytoplankton in the Middle Caspian during the bloom period in early September 2022.
Water samples were taken at different horizons (from the surface to the bottom layer) at a series of stations along the western...
For the past 25 years, flow cytometry has been a gold standard for the direct measurement of viral-like particles (VLP) in aquatic ecosystems. Flow cytometry allows for higher throughput and costs less than alternative enumeration methods, leading to its broad usage in aquatic viral ecology. A major challenge associated with flow cytometry is the d...
Hairy cell leukemia (HCL) is a rare B-cell neoplasm that constitutes around 2 percent of all lymphoid leukemias and occurs more frequently in elderly males. The usual triad of HCL includes pancytopenia, splenomegaly, and hairy cells in the bone marrow. This is a case of an atypical presentation of biclonal HCL diagnosed on flow cytometry; the exist...
Dendritic cells (DCs) play a central role in the initiation of the adaptive immune response. Here, we present a protocol for isolating and transcriptionally profiling antigen-presenting cells (APCs) from the mouse lung and mediastinal lymph nodes (MLNs) following intranasal immunization. We describe steps for preparing single-cell suspensions from...
The difference in the immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in patients with mild versus severe disease remains poorly understood. Recent scientific advances have recognised the vital role of both B cells and T cells; however, many questions remain unanswered, particularly for T cell responses. T cells are...
The evolution of antimicrobial resistance (AMR) presents substantial challenges to global medical health systems. Neisseria gonorrhoeae (N. gonorrhoeae), in particular, has developed resistance to all currently available antimicrobials. Addressing this issue necessitates not only discovering new antimicrobials but also deepening the understanding o...
Deep mass spectrometry-based proteomic profiling of rare cell populations has been constrained by sample input requirements. Here, we present a protocol for droplet-based one-pot preparation for proteomic samples (DROPPS), an accessible low-input platform that generates high-fidelity proteomic profiles of 100–2,500 cells. We describe steps for depo...
Here, we describe protocols for analysis of the inducible protein aggregate agDD-GFP in HeLa-dCas9 and HEK293T cells under a variety of conditions and in various genetic backgrounds. agDD-GFP is stabilized by a small molecule (shield-1, S1) and washout of S1 leads to agDD-GFP aggregation in minutes. By monitoring aggregates after S1 washout by flow...
Through experimental studies, many details of the pathway of integrin $\alpha_{\rm IIb}\beta_3$ activation by ADP during the platelet aggregation process have been mapped out. ADP binds to two separate G protein coupled receptors on platelet surfaces, leading to alterations in the regulation of the small GTPase RAP1. We seek to (1) gain insights in...