Science topic
Fixatives - Science topic
Fixatives are agents employed in the preparation of histologic or pathologic specimens for the purpose of maintaining the existing form and structure of all of the constituent elements. Great numbers of different agents are used; some are also decalcifying and hardening agents. They must quickly kill and coagulate living tissue.
Questions related to Fixatives
NOTE 1 [file ions.mdp]:
With Verlet lists the optimal nstlist is >= 10, with GPUs >= 20. Note
that with the Verlet scheme, nstlist has no effect on the accuracy of
your simulation.
Setting the LD random seed to -397045813
Generated 100474 of the 100576 non-bonded parameter combinations
Generating 1-4 interactions: fudge = 1
Generated 66298 of the 100576 1-4 parameter combinations
Do climate changes cause a decrease in the amount of annual rainfall in dry areas, or do they cause a change in the fluctuation of the dates and intensity of rains? as we notice an increase in cases of floods and torrential torrents in those areas? This leads to the question of the rain isolines, whether they are fixed or variable as a result of the severity of climate changes?
Can orthodontic treatment fix black triangles in teeth?
Human understanding includes different types of thinking, including growth and fixed mindset, analytical cognitions, past or present-oriented observations, and future-thinking notions. In your opinion, what is future-thinking mindset?
Despite going through available literature and getting a basic understanding of how Kernel Density Estimation works when analysing home ranges, I cannot make my Biotas works. Does anybody know about some more detailed guidelines/manuals/training videos etc., for Biotas? Provided manual on their website says very little about navigating within the software and setting required parameters. For instance, choosing a window with (LSCV), I have no idea what units are represented there. In my case, I have a limited dataset, let's say less than 100 GPS fixes of the Little owl, which moves on a small scale. Moreover, it roosts often on the same spots; therefore, my points are dense and sometimes even overlaid. I would like to avoid manipulating hard data and exclude those overlaying points from analyses because I still think they have important information value. Would it be still possible to make a fixed KDE with LSCV under such conditions?
Any help will be highly appreciated. Thx a lot.
Hi!
I plan on doing a JC-10 MMP assay and would like to know if I can add it to my flow cytometry panel. My protocol includes surface and intracellular staining (Fix/Perm Foxp3 eBioscience kit). I know JC-10 only works on live cells. I plan to first incubate the cells with the JC-10 reagents (as recommended by the manufacturer) and then do my regular flow protocol on these cells to ultimately run them on the cytometer.
Would the fix/perm step interfere with the JC-10 assay? Should I run the JC-10 assay separately from my flow panel?
Thanks!
Banach's Fixed Point Theorem (also known as Contraction Mapping Theorem) can be applied to prove the existence and uniqueness of solutions to differential equations with boundary conditions by transforming the differential equation into a fixed-point problem in an appropriate function space.
what are the key steps of this process?
Hi everyone
I accidently deleted the cas.h5 file about a simulation of CFD. I recovered file by Recuva and when I opened it this error appeared. How can i fix this error.
Thanks everyone
Relative Permeability (RP): Has anything to do with Darcy’s Law?
RP: Most important and least understood property (we only have a limited number of well-characterized studies on RP)
RP: Manifestation of complicated pore-level displacement physics along with the characteristics of fluid-fluid and fluid-solid interactions
RP: The actual measurement poses a real challenge
(a)By using laboratory-scale experiments using representative rock-fluid systems
(3-phase RP is almost exorbitantly difficult, costly & time-consuming);
(For a given rock-fluid system and under fixed experimental conditions, RPs are not unique functions of phase saturations, but would depend upon saturation history)
(The problem of saturation history in 3-phase flows is more convoluted since there are 2 independent phase saturations, increasing the number of process paths significantly)
(We have more than 2 saturation histories in 3-phase flow, as the saturation of each phase may increase, or, decrease, or, may remain unchanged) OR,
(b) By using Empirical Correlations; OR,
(c) By using Physically-based Pore-Scale Models
(d) (b) & (c) require high-quality experimental data for development and validation
Even if we assume the Reservoir to be Water-Wet, RP of the most wetting-phase remains to be independent of saturation history, however, the RPs of the intermediate-wetting and the most non-wetting phases remain significantly affected by saturation history.
Hello everyone, I am using overset mesh for a FSI case, and since the simulation takes a long time, I occasionally have to cancel and restart it from the latest time step. However, when I restart, the airfoil's center of mass reverts to its initial position rather than continuing from the last position before stopping. Has anyone encountered a similar issue or know of a solution? I have attached the vertical movement of the airfoil for both the uninterrupted case and the situation where the simulation was restarted.
Hello everyone,
I am planning to apply a two-stage DEA model to assess the efficiency of banks. For Stage 1, the input variables include number of employees, total fixed assets, and total operating expenses. The output of Stage 1 and input of Stage 2 are total deposits and total loans. Finally, the outputs of Stage 2 are interest income, non-interest income, and non-performing loans.
I am aware that there are several approaches to selecting variables for a DEA model, such as the intermediation approach, production approach, and profitability approach... among others. However, I am unsure which approach best fits the way I have chosen the variables for the two stages of my DEA model. Could anyone suggest which approach I am following with this variable selection and how I should frame it in my research?
I would greatly appreciate any help or suggestions. Thank you!
The Problems with Layering Anterior Composites and How to Fix Them ?
Hello. When trying to record CV, after the voltage transitions to the negative region, a sharp jump in current is observed. Increasing the concentration of electrolyte, analyte, or electrode area does not help. Sweep speed 100 mV/s, electrolyte concentration 0.1 M. What could be causing this phenomenon and how can I fix it?
The last author on my recent publication (intra-arterial verapamil vs. nicardipine) is wrong? Can you please update/change this? The author is listed correctly in pubmed, thanks!
Keshav Patel
To induce polarization and study of drain current.
What causes some stars to appear fixed while others seem to move across the night sky and stars appear very bright in the sky?
Can anyone lease tell me what is the best way to attach agarose beads on a cover glass so that it doesn't move from it's place? Is there any coating material that can hold the beads in it's place?
Dear researchers
I have salmonella Florenced labeled bacteria and want to do invasion (caco2 and LMH cells) and then fixing for evaluating with microscope
I have problem in fixing could you please let me know about the best protocol which solve my problem and successfully fixed the cells
Thank alot in advance
Cheers
What would happen if nitrogen-fixing plants could no longer fix nitrogen from the atmosphere and what would happen if nitrogen-fixing bacteria disappeared?
Application of neural networks in shape prediction
Is there a fixed code used in shape prediction in neural networks?
I fixed it with 4% paraformaldehyde and then stained it with DAPI.
Hi everyone, I'm currently working on an autodock4 and I've received those warning in my running process. I don't know how to fix or how it affects to my final results. Attached below are my dpf file and cmd warning.
Thanks for your advices.
seti or search of intelgenece articial nee to be fixed due to masss solar systems outhere thta expelled laser beam light ollimated wel beign so is neede to modificated the seti in a new device of armenta velasquez like radi ho can detect the laser signals
In the fight against global warming, does the biochar really have the capacity to absorb more carbon than it takes to produce it and biochar help fix climate change?
I've trimmed my sequences in Geneious, exported them as fasta documents, and imported them into fasta. I'd like to use Mesquite to align my sequences. To do so, I downloaded Muscle align from Github and keep running into this error: MUSCLE Align quit, possibly because of an error (1). Please examine StandardOutputFile and StandardErrorFile in the analysis directory for information.
The StandErrorFile says :
Invalid command line
Unknown option in
Help appreciated!
I am looking to stain live Saccharomyces cerevisiae cells using DAPI (Thermo cat # 62248) but have currently been unsuccessful. Does anyone have a protocol to stain these cells without having to fix them using either DAPI or Hoechst, I want to be able to visualize the nucleus in live cell? Also does it matter if you use DAPI or Hoechst, is one better than the other?
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze it while the other half is fixed.
What should be the best protocol to follow?
Hey..I want to start electrocatalysis for small molecule activation, but the problem is that I am not getting that how do I fix the scan rate. I read some literature where in some of them people are keeping 0.1 V/s, somewhere 0.05 V/s, or somewhere 1.0 V/s, hence I am confused.
Kindly help me in this regard.
Thanks for giving your valuable time and considerations.
How do we fix the appearing problem in VNA? The attached window appears during the calibration, and in the end, the measurement results cannot be viewed.
I want to fix it to run APEA, the problem is when I run the APEA in the heater give me a error, but the temperatura difference is about 160 F.
Someone can help me?
The simulation is in Hysys
Excuse me about my English language skills
For a grid connected BESS (lets say 100 MW/200 MWh system, LFP battery), if we charge the BESS in such a way that whenever we can access power from the solar that will charge the BESS. Hence it can be like, the BESS can have 10 partial charging and 10 partial discharging per day. Instead of direct 2 hours of fixed charging and 2 hours of fixed discharging, does these sort of continuous partial charge-discharge do any harm to the BESS in terms of degradation, performance, or any other technical issue?
Look forward to your answer. Thanks in advance.
Hi users,
I want to create an itp file for my ligand (ligand with ruthenium) complex, im using MCPB.Py for generating itp for my structure during the process i got the following error kindly please help.
MCPB.py -i dna.in -s 3
The input file you are using is : dna.in
The following is the input variable you have:
The variable ion_ids is : [1201]
The variable ion_info is : []
The variable ion_mol2files is : ['Ru.mol2']
The variable original_pdb is : complex_fixed_h.pdb
The variable add_bonded_pairs is : []
The variable add_redcrd is : 0
The variable additional_resids is : []
The variable anglefc_avg is : 0
The variable bondfc_avg is : 0
The variable chgfix_resids is : []
The variable cut_off is : 2.8
The variable force_field is : ff19SB
The variable frcmod_files is : ['LIG.frcmod']
The variable gaff is : 1
The variable group_name is : dna
The variable ion_paraset is : 12_6 (Only for the ions using the nonbonded model).
The variable large_opt is : 1
The variable lgmodel_chg is : -99
The variable lgmodel_spin is : -99
-99 means program will assign a charge automatically.
The variable naa_mol2files is : ['LIG.mol2']
The variable scale_factor is : 1.0
ATTENTION: This is the scale factor of frequency. The
force constants will be scaled by multiplying the square
of scale_factor.
The variable smmodel_chg is : -99
The variable smmodel_spin is : -99
-99 means program will assign a charge automatically.
The variable software_version is : gau
The variable sqm_opt is : 0
The variable water_model is : OPC
The variable xstru is : 0
******************************************************************
* *
*======================RESP Charge fitting=======================*
* *
******************************************************************
***Generating the 1st stage resp charge fitting input file...
***Generating the 2nd stage resp charge fitting input file...
***Doing the RESP charge fiting...
=========================Checking models==========================
***Check the large model...
Good. The charges and atom numbers are match for the large model.
Good. There are 27 atoms in the large model.
***Check the standard model...
Traceback (most recent call last):
File "/home/saranya/amber20/bin/MCPB.py", line 692, in <module>
resp_fitting(stpdbf, lgpdbf, stfpf, lgfpf, mklogf, ionids, ff_choice,
File "/home/saranya/amber20/lib/python3.9/site-packages/pymsmt/mcpb/resp_fitting.py", line 521, in resp_fitting
raise pymsmtError('Error: the charges and atom numbers are mismatch '
pymsmt.exp.pymsmtError: Error: the charges and atom numbers are mismatch for the standard model!
I have calculated maximum debonding force between sma fiber and epoxy for a fixed interfacial shear strength but in one paper author plotted interfacial bond strength. Can anyone tell me how to get interfacial bond strength in ansys
Hi everyone,
I ran a Generalised Linear Mixed Model to see if an intervention condition (video 1, video 2, control) had any impact on an outcome measure across time (baseline, immediate post-test and follow-up). I am having trouble interpreting the Fixed Coefficients table. Can anyone help?
Also, why are the last four lines empty?
Thanks in advance!
Hello, I am trying to fix Pseudomonas aeruginosa PAO1 cells with 4% formaldehyde for 20-30 minutes (on ice and in the dark) for fluorescent microscopy. I am attempting to stain the DNA and outer membrane, but have not been successful with this fixation method. The dyes give weak signals and appear to be exported out, suggesting the bacteria are not completely fixed with formaldehyde. Does anyone have a reliable protocol for PAO1 fixation, including concentration, time, quenching, etc.? Any help would be greatly appreciated.
how to fix this error,
from phq_readin : error # 1
DFPT with the Blochl correction is not implemented
I am attaching my ph.in file.
Hi everyone
I have got this test in a fixed excitation and various emissions. In some wavelengths there are overflows which make the resulted analyze different from other carbon dots.
Is it normal or there are some issues?
I have a problem with geo-referencing a shape file. How can I fix it when it appears in mines despite selecting WGSWGS 84 / UTM zone 38N?
We are currently trying to calculate the lattice thermal conductivity of several metals (Ni, Cu, Pd, Ag, Pt and Au) using the non-equilibrium Müller-Plathe method in LAMMPS. We are considering big supercells (20x20x20 unit cells, 70k+ atoms) for each elemental metal separately. The interactions between atoms are mediated by the well-known MEAM potential. The heat flux is tallied using the fix thermal/conductivity command, and temperature profiles are recorded using the compute chunk/atom and fix ave/chunk commands. Please find attached the typical input run for reference. We have successfully run this for other systems (molecular liquids governed by OPLS-AA), but for MEAM metals we found that the temperature profiles are basically erratic noise. While the lattice thermal conductivity of metals should low, we do not expect it to be completely negligible.
Is anyone aware of existing problems with the application of the Müller-Plathe method together with the MEAM potential? Is there particular requirements for such a case? We have played with the Nevery and Nswap parameters in the fix thermal/conductivity command with no success.
Thank you in advance for your attention.
*****
include "system.in.init"
read_data "system.data"
include "system.in.settings"
include "system.in.charges"
neigh_modify every 1 delay 0 check yes
variable t equal 0.001
variable T equal 298.0
variable P equal 1.013
variable s equal 5
variable c equal 10000
variable d equal $s*$c
variable kB equal 8.617333262E−5
minimize 1.0e-6 1.0e-8 1000 100000
reset_timestep 0
timestep $t
thermo_style custom step etotal temp press lx ly lz density
thermo 1000
fix 1 all nvt temp $T $T 10.0
run 500000
unfix 1
fix 2 all npt temp $T $T 10.0 iso $P $P 100.0
run 2000000
unfix 2
reset_timestep 0
thermo_style custom step etotal epair ke temp press
thermo 1000
fix 3 all nvt temp $T $T 10.0
run 10000000
unfix 3
reset_timestep 0
fix 4 all nve
fix 5 all thermal/conductivity 100 z 50
compute ke all ke/atom
variable temp atom c_ke/(1.5*${kB})
compute layers all chunk/atom bin/1d z lower 0.02 units reduced
fix MP all ave/chunk $s $c $d layers v_temp file temp.profile ave one
thermo_style custom step etotal epair ke temp press f_5
thermo 1000
run 10000000
unfix MP
unfix 5
unfix 4
Hi there,
I am trying to fix, stain, and image cells in mitosis with an antibody against gamma-H2AX. I do not synchronize my cells as part of my research looks at genome integrity, so I usually just grow them to a higher confluency and search for cells in metaphase or anaphase manually. I have been fixing with 4% formaldehyde at 37C for 10 minutes, and blocking with 5%BSA in TBS+0.01%Triton-X. I do this at 37C because I don't want the microtubules to depolymerize in response to cold fixative. However, I always seem to get non-specific background staining. There are a lot of little foci all over the place. I notice that they frequently colocalize with microtubules on the spindle, but I don't know why that would be happening. Would it be better if I used Ice cold methanol instead? Would this ruin any analysis I do on cells in metaphase in anaphase due to microtubule depolymerization?
Thanks!
Hi!
I was opening CST file, it showed this problem.
Do anyone know how to fix it?
I have tried restart my computer, but it didn't work.
"epigenetic information is stored in a digital-analog format, susceptible to alterations induced by diverse environmental signals and cellular damage" and potentially polish can fix those scratches(
I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci outside the nuclei. Instead, there's a slightly cloudy blue color in the center of the image. Could this indicate mycoplasma contamination? Thanks!
Hi everyone! I tried to perform a classic One Way Anova with the package GAD in R, followed by a SNK test, which I always used, but it didn't work with this dataset, and I got the same error for both tests, which is the following:
"Error in if (colnames(tm.class)[j] == "fixed") tm.final[i, j] = 0 :
missing value where TRUE/FALSE needed"
I understand there is something that gives NA values in my datatset but I do not know how to fix it. There are no NA values in the dataset as itself. Here is the dataset:
temp Filtr_eff
gradi19 11.33
gradi19 15.90
gradi19 10.54
gradi26 11.01
gradi26 -1.33
gradi26 9.80
gradi30 -49.77
gradi30 -42.05
gradi30 -32.03
So, I have three different levels of the factor temp (gradi19, gradi26 and gradi30) and my variable is Filtr_eff. I also already set the factor as fixed.
Please help me, how do I fix the error? I could do the Anova with another package (library car worked for example with this dataset) and I could do tukey instead of SNK, but I want to understand why I got this error since it never happened to me..thanks!
PS: I attached the R and txt files
I have a problem, in additive manufacturing (here specifically LPBF), if you make parts with different shapes through a fixed printing process, the parts will deform due to different geometry resulting in different stress concentrations. However, for the same shape of the part, the use of different printing process parameters to manufacture the part, will have an impact on its deformation? Such as different laser power, scanning rate, etc. (of course, if the part can be successfully manufactured)
Hello everyone,
I am very much new in quantum espresso and Boltztrap2. I have faced a problem regarding the fermi energy of semiconductor.I have done scf and nscf calculation for Perovskite material. But there in the nscf file output I have got HOMO and LUMO , not the fermi energy as it is a semiconductor material . As per Quantum Espresso input file description for semiconductor and insulator occupation will be fixed. but using this i cannot get the Fermi energy . In the boltztrap calculation I have to give the fermi energy value. Can you please suggest what should I do?
I'm trying to fitting Cu Auger peak into various state (Cu0, Cu+, CuI etc..)
In Cu0, one of the peak's FWHM is 0.84 but when I try to enter smaller value than 0.89, "input error" is occured. How can I put smaller value?
And plus how can I 'fix' the FWHM of other values? Whenever I try to optimize peaks, the values are changing.
Will this affect properties such as band and DOS? If yes, then how can I fix this? This calculation has been done in WIEN2K.
I need a PET + Solvent mixture to feed it to a fixed bed reactor for steam reforming.
How to store SDS-PAGE for fluorescent imaging without fixatives?
I am running protein samples on SDS-PAGE that are tagged with fluorophores. If I place the gel in a destain solution of 60%water/30%methanol/10% acetic acid, the chemistry reverses and I lose the fluorophore. Is there an alternative storage solution I can use to get my gel to the imager to capture the fluorescence without chemical fixatives? After imaging, I then proceed to Coomassie Blue staining in acetic acid and methanol, at which point I am not worried about losing the fluorophore.
I have samples with monocytes and THP-1 cells fixed in paraformaldehyde 1% and would like to stain with these fluorescent probes if it is possible.
What is the role of technology in mitigating climate change and which is a technological fix to mitigating climate change?
I'm currently trying to do some EMSA using P32. The first two gels looked perfect, with sharp bands and no background. However, all the following ones looked like the blot below. I'm not doing anything different. All my buffers and gels were freshly prepared, and I even produced fresh nuclear extracts to make sure my other batch was not degraded, and it still looks like that. So far, I've repeated it five times, but nothing's changed. Has anyone had the same problem or an idea of what I can do?
Traceback (most recent call last):
File "/home/aarushi/gromacs/cgenff_charmm2gmx_py3_nx2.py", line 54, in <module>
import networkx as nx
File "/home/aarushi/gromacs/myenv/lib/python3.10/site-packages/networkx/__init__.py", line 114, in <module>
import networkx.generators
File "/home/aarushi/gromacs/myenv/lib/python3.10/site-packages/networkx/generators/__init__.py", line 14, in <module>
from networkx.generators.intersection import *
File "/home/aarushi/gromacs/myenv/lib/python3.10/site-packages/networkx/generators/intersection.py", line 13, in <module>
from networkx.algorithms import bipartite
File "/home/aarushi/gromacs/myenv/lib/python3.10/site-packages/networkx/algorithms/__init__.py", line 16, in <module>
from networkx.algorithms.dag import *
File "/home/aarushi/gromacs/myenv/lib/python3.10/site-packages/networkx/algorithms/dag.py", line 23, in <module>
from fractions import gcd
ImportError: cannot import name 'gcd' from 'fractions' (/usr/lib/python3.10(m(m((((((((myenv)
could you please help me fixing this error?
I have scan data of multiple individuals over a while and
their positional photographs were taken at certain fixed instances during focal sampling. I want to obtain the inter-individual distances of these sets of individuals from the scan data. Can any software help me extract this positional data for the focal individuals from these photographs?
Thank you so much for your attention and participation.
I have to remove OCT from the tissue completely and fix the OCT-removed tissue in paraffin. Please suggest the best way.
I've run it three times to try different parameters, but it stops changing after running for a short while. Please help me understand how to fix this. Is there a problem with the sequence or the parameters?
6471000 -- (-192324.911) [-190903.882] (-191836.670) (-203192.846) * (-192291.336) (-195396.079) (-197058.880) [-190667.824] -- 36:24:32
6472000 -- (-192324.911) [-190903.882] (-191836.670) (-203199.097) * (-192291.336) (-195396.079) (-197058.880) [-190667.824] -- 36:23:56
6473000 -- (-192324.911) [-190903.882] (-191836.670) (-203206.485) * (-192291.336) (-195396.079) (-197058.880) [-190667.824] -- 36:23:22
6474000 -- (-192324.911) [-190903.882] (-191836.670) (-203186.976) * (-192291.336) (-195396.079) (-197058.880) [-190667.824] -- 36:22:47
6475000 -- (-192324.911) [-190903.882] (-191836.670) (-203176.349) * (-192291.336) (-195396.079) (-197058.880) [-190667.824] -- 36:22:12
Average standard deviation of split frequencies: 0.143548
6476000 -- (-192324.911) [-190903.882] (-191836.670) (-203174.778) * (-192291.336) (-195396.079) (-197058.880) [-190667.824] -- 36:21:37
6477000 -- (-192324.911) [-190903.882] (-191836.670) (-203190.326) * (-192291.336) (-195396.079) (-197058.880) [-190667.824] -- 36:21:02
6478000 -- (-192324.911) [-190903.882] (-191836.670) (-203201.748) * (-192291.336) (-195396.079) (-197058.880) [-190667.824] -- 36:20:27
6479000 -- (-192324.911) [-190903.882] (-191836.670) (-203196.704) * (-192291.336) (-195396.079) (-197058.880) [-190667.824] -- 36:19:52
6480000 -- (-192324.911) [-190903.882] (-191836.670) (-203173.040) * (-192291.336) (-195396.079) (-197058.880) [-190667.824] -- 36:19:18
Average standard deviation of split frequencies: 0.143548
6481000 -- (-192324.911) [-190903.882] (-191836.670) (-203178.787) * (-192291.336) (-195396.079) (-197058.880) [-190667.824] -- 36:18:43
6482000 -- (-192324.911) [-190903.882] (-191836.670) (-203151.441) * (-192291.336) (-195396.079) (-197058.880) [-190667.824] -- 36:18:08
6483000 -- (-192324.911) [-190903.882] (-191836.670) (-203159.321) * (-192291.336) (-195396.079) (-197058.880) [-190667.824] -- 36:17:33
6484000 -- (-192324.911) [-190903.882] (-191836.670) (-203156.963) * (-192291.336) (-195396.079) (-197058.880) [-190667.824] -- 36:16:58
6485000 -- (-192324.911) [-190903.882] (-191836.670) (-203142.242) * (-192291.336) (-195396.079) (-197058.880) [-190667.824] -- 36:16:24
Average standard deviation of split frequencies: 0.143548
So let's say I wanted to do a research with this topic:
"Factors affecting QOL of beta-thalassemic patients"
Study Design: Cross Sectional
Number of Independent Variables: 21
Dependent Variables: mean QOL score
Statistical Analysis: Descriptive statistics, bivariate analysis, followed by multiple linear regression
Power: 80%
Significance level: 0.05
Aim of study: figuring out which of the 21 independent variables included significantly affect the QOL of beta-thalassemic patients
First of all, is it correct for me to use G*power F test to do a power analysis with A priori to determine my minimum sample size or should I be using something else?
If yes, then which test do I use? Do I use the "Linear multiple regression: fixed model, R-squared deviation from zero", or "Linear multiple regression: fixed model, R-squared increase" or should I use something else entirely? Thank you for helping out!
Is there any way to somehow normalize these coefficients? How can I fix this problem?
I have been trying to install PowerMarker on my Windows 10 PC but it is not working.
One of the requirements for PowerMarker installation is MS NET Framework 1.1.4322 condition, however, Windows 10 no longer supports this 1.1.4322 version.
I'd need assistance, please.
Hi everyone,
I want to Apply Tests for Special Causes in p-chart, but the sample size for each group is different. So I don’t have a fixed UCL and LCL. Just a question can I apply Tests for Special Causes? Can I calculate index when I don’t have a fixed LCL and UCL.
Thanks,
Hi, i am currentyl fitting mno2 material into the peak at Mn 2p 3/2 and Mn 2p 1/2. in the right side of the peak, the tip highlighted in red is still not fit enough. is there any idea how to fit the top so that the black lines all matched the blue raw data lines?I already estimated the initial value and the fix it and then run the first itenery. after that i ticked of the fix button and let it run again. But it is still not fit enough.
Thank You very much for your kind assistance
I know that it is recommended during fixing column but I am curious if there are much more cases. Maybe it has a lot of applications ;)
1 ton charcoal fixes 3,7 t CO2. The global charcoal production is 54 mio tons, in which 200 mio t CO2 are fixed. 200 times the currently produced charcoal will fix the entire CO2 emitted globally per year!!! CO2 emission about 30 bln t per year: means this will be fixed in 10 bln t charcoal.
The entire annually CO2 can be fixed in a volume 2,7 km * 2,7 km*2,7 km and could be dumped in e.g. open pit mining sites where the coal was exploited (give it back).
Rapid growing biomass may be used. Charcoal production cost may be assumed the in the order of 200$ per ton, results in an annual investment of 2000 bln $ globally. THIS IS THE SAME AS WHAT ARE MILITARY WEAPON EXPENDITURES.
This is possible...in principle!
Please tell me where I am wrong in my rough brainstorming.
Hi,
I am trying to do an Ordinal Logistic Regression (OLR) in R since this is the regression analysis that I need to use for my research.
I followed the tutorial video I found in YouTube in setting up the two sample models. Here are the 2 codes for the models:
modelnull <- clm(as.factor(PRODPRESENT)~1,
data = Ordinaldf,
link = "logit")
model1 <- clm(as.factor(PRODPRESENT)~Age+ as.factor(Gender)+Civil Status,
data = Ordinaldf,
link = "logit")
Next, I followed what the instructor did in doing anova and an error message prompted. It says, Error in UseMethod("anova") : no applicable method for 'anova' applied to an object of class "c('double', 'numeric')"
Is there something wrong in setting up the two sample models, hence, an error message in prompting? What needs to be done to fix the error?
Please help.
Thank you in advance.