Questions related to Fish Biology
Dear researchgate members,
I recently made two attempts to grow the aquarium plant Cryptocoryne wendtii emersed, i.e., outside of water. Unfortunately, both attempts failed, and I am unsure of what went wrong.
In the first attempt, I heated soil in the oven and shaped it into a cube. I then placed the aquarium plant into this cube. In the second attempt, I used rock wool instead. In both cases, I lightly moistened the soil and rock wool with aquarium water. Subsequently, I placed them in plastic bags and provided CO2 by exhaling into the bags through a straw. The bags were sealed with rubber bands and positioned under an LED strip light. The distance between the light and the plants was approximately 10 cm, ensuring that the light intensity was not harmful.
After one week, I exchanged the air inside the bags and provided more CO2 by breathing into them again. Unfortunately, after two weeks, I couldn't observe any positive results. Almost all the plants in both the soil and rock wool died. There was no growth observed, neither in the plants themselves nor in the roots.
I am very confused and frustrated, as I don't understand where the mistake lies. Do you have any ideas or advice on what I might have done wrong? Are there specific conditions that I should consider to achieve successful emersed cultivation of Cryptocoryne wendtii?
I would greatly appreciate your help and support! Thank you!
What are some potential business opportunities that can be created by implementing a smart water quality prediction system for biofloc aquaculture, and how can they be leveraged to create a competitive advantage in the aquaculture industry?
Here attached caudal fin X-ray image of Ameiurus nebulosus (Pisces: Siluriformes).The number of caudal fin rays in descriptions is among 18-20, but nobody tells how he count it. I see 8+8 principal rays (on hypurals+parhypural) + 35 procurrent. Or I can count 6 segmented unbranched, 15 segmented branched and 30 unsegmented rays. O'k, I have 6, 15 and 30. What I must do with these numbers to get in range 18-20? It is not the only one, I have dozens of such tails.
Second image is with how I see the structure of caudal fin of other A. nebulosus.
It is very hard for me to find journals to publish data on the actual status of fish fauna distribution from rivers in Romania. Most of the journals (with IF) reject papers on fish taxonomy and diversity indices. If this happened to you, please name the journal and also if you have published this type of article, please name the journal as well.
I'm wondering if some of you are aware of existing conversion factors for levels of metallic contamination in fish muscle tissues. In the present work, I'm focusing on metallic contamination, so not currently interested in lipid content of the tissues.
Depending of the contexts, concentrations are expressed relatively to fresh or dry weight. By example, working on muscle sample is more convenient when dried, but concentrations are expressed relatively to wet weigh in European directives, requiring conversion factors.
In most of the paper I read, concentrations are expressed relatively to wet or dry weight, and are then converted using a 5 times conversion ratio. But no information about the actual measurement of the ratio is provided, and I feel this value is largely empirical.
So, is someone aware of the rationale for this value ? Are you aware of papers specifically investigating this point ?
I read many available articles but none of them concretely wrote this down. Most of them determined a spawning period but I need more specific data to calculate the reproduction rate for DEB-IPM.
Sorry if the answer to this question is very obvious, I am fairly new on this field.
This is similar to a previous question I asked. I'm looking for some kind of chemical marker to use for an antibody test to determine where on a fish an unrecognizable sample of tissue came from. All the AMPs, etc. that my research has turned up so far are either pretty species-specific or are also present in the GI tract. Any help would be extremely appreciated.
Please provide me with any information about the fish [Nebbash (Barbus schejeh)]. This specie is of the family Cyprinidae,
With my best regards
Dr M. H. Matllob
I have a historical collection of otoliths mounted in both 1,2-dichloroethane and cytoseal which I'd like to remove for detailed shape analysis. I've seen toluene recommended online but can't find any exact methodology. I've also been told xylene may work. Wondering if anyone can give any recommendations?
FMC (FORMALDIHYDE, MACHITE GREEN ,METHALINE BLUE)
FMCS (FORMALDEHYDE ,MALACHITE GREEN , COPER SULPHATE ) The second equation is correct or not ? so please informs
I'm wondering if someone is aware of studies investigating the integration time of mercury in fish tissues, mostly muscle. In other words, when a Hg concentration is measured in muscle, is it the result of previous contamination during weeks? months ? years ? I found some papers investigating Hg dynamics in mammals or birds, but I can't find similar studies in fish. This information would be crucial when trying to infer trophic patterns from several biomarkers (eg stable isotopes, fatty acids or contaminants) with different integration time.
I have data of catch and catch per unit effort of cuttlefish for 2 years. The data came from artisanal hook and line fishery. Can we simply use the equation which relate CPUE and abundance to estimate fish biomass? How to estimate the catchability coefficient in such case? There are many factors affecting this relationship and sometimes it is difficult to take account of them.
The data I have include catch, effort in time spent fishing (from handheld gps loggers) and the estimated CPUE.
Particularlythe b12 in spirulina absorbed by the fish and used? I know for humans it is argued not to be, but is it potentially useful in providing fish or zooplankton, with usable b12 supplement. By using live freshly grown spirulina.
Looking at dosing marine zooplankton (copepods) with spirulina and the nutritional affects they have and the ability to pass them on. Furthermore, looking at any particular nutritional benefit soaking food in spirulina may have, etc etc.
We need to perform a screen for heat shock response activation and I can't find an european source for a simple Tg(Hsp70:GFP) line. The source should preferably be in the EU, because we've had unpleasant experiences with border delays after shipping some planarian lines from the US and I'm not sure embryos would survive.
EZRC has Tg(Hsp70:EGFP-HRAS), I suppose we could use those, but wouldn't that make the methodology seem too messy?
Dose anybody have some photos from below crab species? I could not find.
The correlation between fish (freshwater) bioaccumulation to damage fish organs (gills, kidney, liver, mussels) it seen biopsy? if possible means why it occurred what is function happened in biological activity... can explained in detail to discussion of biological actions.
Normally freshwater fish meristic character of fin ray counts example dorsal fin or caudal fin rays count variability of same within species it possible or not if possible why it occurred.
I am designing a study examining burst (maximum short period fast-start swimming) swimming in a range of fish species in a swim chamber. Many of the older papers used electric stimulus to provoke burst swimming. To me this looks like the most consistent and therefore repeatable method but I'm running into animal ethics difficulties.
Some of the newer articles use other physical stimuli (e.g. tapping the chamber, squirting with a pipette etc) to provoke burst swimming but I worry about the repeatability of these methods. Other papers purport to provoke burst swimming by rapidly increasing velocity but this must involve some level of fatigue and depends very much on the behavioral response of the species in question.
We have measured whole-body copper content in tilapia larvae when their yolk sac were almost disappeared (fry). At that time, there was no external feeding. We found that a significant bioaccumulation of Cu while the Cu concentration in water was not detectable (<0.001 ppb). We therefore supposed that the origin of Cu might be related to its concentration in yolk.
Please let me know if you have any similar experiences.
I saw the formula above the question, but I can't understand in detail, any one can explain in detail above the formula along with how to analysis with statistical software example using EXCEL or any other software is there pls explain...
I have a little statistical problem to resolve. Actually I have data with distances between a target fish and three prey (quantitative dependent variable) in function of time (quantitative input also) and I found that it is called a time serie.
My question is how to compare these three time series ? Graphically, when target fish is attracted by one of the prey, the distance between them decrease dramatically while distance with the two other preys is constant or increase slightly. How can I compare these thresse series statistically and how can I determinate statistically the time at which fish is attracted by one of the three preys ?
Thank you for answering me
For human health we use a number of general and specific tests that help understand the condition of people. Have any such tests been done and tested for fish?
Basically is there any way you can think of to ascertain that a fish caught from the wild has been feeding on the slime coats of other fishes opportunistically without having observed its behaviour? Is it even possible? I suppose an antibody test of some sort might work but that would be expensive and time-consuming to create. This is an idea I'm hoping to incorporate into my potential future master's thesis.
Does anyone know where(online) I can get a complete fish cell culture protocol, specifically for salmon head kidney cell?
Is there any type of circadian clock rhythmic expression happens due to different colors of light? Or different intensity of the light can affects?
Kindly give me any suggestion.
I'm looking to conduct an experiment examining how the dopaminergic system may influence the decision to strike or avoid a fishing lure in sportfish. I'm looking to use D1/D2 receptor agonists (SKF-38393 hydrochloride/Quinperole hydrochloride) and antagonists (R-(+)-SCH hydrochloride/Eticlopride hydrochloride) to manipulate the DA systems in these fish. My question is a methodological one. After mixing the solutions and immersing a fish, can I preserve the solutions for subsequent re-use, even over a period of a few days of trials? Or, do I need to mix a fresh solution for each fish?
For estimating Lm, different researchers uses different methodologies. Some uses the lowest recorded mature fish as Lm. Some researchers estimated it by eye observation of visible egg. Some estimates from the first peak og GSI. Some uses cumulative percentage of all samples of fully matured egg (Stage v and above) to estimate Lm. But is there any method to calculate Lm based on histological stages (i-vii) and maturity stages i.e., cumulative percentage of samples over certain maturity stages (stages i-vii/viii) ?
waiting for your innovative ideas......
The encephalization quotient (QE) are calculated for each species using the allometric equation describing the brain mass to body mass relationship (modified from Lisney & Collin, 2006). The brain metrics can considers "brain areas (the olfactory bulbs, telencephalon, optic tectum, corpus cerebellum and the octavolateralis area)" - Lisney & Collin, 2006) - or the total brain or the spinal chord. Inasmatch QE is defended and criticized by different academicists, even in mammals researches, can we use QE to analyses freshwater fish communities (using the community ecology framework)?
Lisney, T. J. and Collin, S. P. (2006), Brain morphology in large pelagic fishes: a comparison between sharks and teleosts. Journal of Fish Biology, 68: 532–554. doi:10.1111/j.0022-1112.2006.00940.x
Jerison, H. J. (1977), THE THEORY OF ENCEPHALIZATION. Annals of the New York Academy of Sciences, 299: 146–160. doi:10.1111/j.1749-6632.1977.tb41903.x
I'm interested in opinions of experts on the age of this sample Alaska plaice (Pleuronectes quadrituberculatus) from south-western part of the Bering sea. My preliminary estimate is 13 years.
Thank you in advance.
I am working on the osteology of the fish species John Dor, Zeus faber from different countries.
I appreciate very much the assistant of any friend who can get me an x-ray fro 3 specimens of the fish species Zeus faber from the waters of the following countries:
Brazil, Uruguay, Argentina, Chile, Peru, Ecuador, Namibia, Congo, New Caledonia, Fiji, and Papua New Guinea
Laith A. Jawad
My male Brown banded bamboo shark has now got a scattering of very small black spots round its head just forward of the gill slits that I've never seen before and he was slightly more reluctant to feed.
I am a little concerned and was wondering if there were any connections and that the black spots were some form of parasite or illness as the moray I keep in the same tank was recently very pale and is still refusing to feed despite his colour mostly returning.
I would get a picture of the spots but unfortunately. my bamboo sharks are far from photogenic and won't stay still long enough for me to get anything more than a very blurred mess
I'm relating the occurrence of micr plastic in the gut of fish species to its total length to see if there's a relation. I feel that the gape size in relation to the micro plastic would be a better comparison to make and therefore, using the data of total length and occurrence of micro plastic, is it possible to obtain a gape size?
Both steelhead and rainbow trout are the same species Oncorhynchus mykiss. What I thought is Steelhead trout migrate from the ocean up rivers to spawn like salmon, and live the majority of their life in the Ocean, at high salinity. While rainbow trout live their entire life cycle in fresh water. Why is it then that freshwater RAS system produce Steelhead, as well as Fresh water lake aquaculture operations and Wild lake fisheries?
I got this image of a ray for ID. The disc is wedge-shaped, this would be typical for guitarfishes. The TL of this specimen is about 20 cm. I have no other images! I never saw a ray with such a coloration.
Place of finding: Harnay Murud sandy beach, India, Arabaian Sea
Thanks for your ideas and comments!
I'm trying to determine a quick and easy way to simply compare the quantity of sialoglycoproteins in fish seminal fluid between two groups, so I don't necessarily need to quantify proteins as accurately as would be accomplished with mass spec or something similar. I would be able to access some optical instruments if necessary (e.g. spectrophotometer, plate reader, etc.). Previously stained these proteins with alcian blue, but looking for a way to easily quantify for comparison.
I am working on fish bacteriology band presently facing problems in analysis of SEM photographs
The degree of toxicity of tetraodontoxin TTX in Tetraodontiform fishes varies by species, and also according to geographic area and season. I want to know the toxicity of the species Ephippion guttifer.
We have a collection of around 70 species specimens from 20 families; all from Vembanad Lake, Kerala, India. We would like to know what all are the effective long term preservation techniques, either using Isopropyl alcohol or something else; except Ethanol (due to availability issues in Kerala). We would like to display these wet specimens too, since there are no collections of this kind available in the vicinity.
The recent revision of Pristis synonymized several former species into Pristis pristis (Faria et al 2013). However the former northern limit of this species in the Western Atlantic is not clear, specially since all sawfish species have suffered dramatic range collapses globally and are in the brink of extinction.
Does anyone know what is the most northern confirmed occurrence of P. pristis in the Gulf of Mexico? Unfortunately all of the museum specimens seen by Faria et al. (2013) from the Gulf of Mexico had NO specific locality!...
I search into Articles and there is no detailed information for age at maturity calculation protocols, and I didn't calculate GSI because my sampling wasn't monthly basis. So Is that make any trouble in that calculation?
I have two main questions. I am working on the amplification of several mitochondrial and ribosomal markers for fish DNA. As I want to sequence afterwards, I was looking for a PCR enzyme with a high fidelity. I saw the Platinium SuperFi (Invitrogen) and got a free sample to test my primers (3 mitochondrial, 1 ribosomal). I tested these 4 markers and only one worked (clear band on the gel after electrophoresis).
These primers are coming from the literature and have been used on fish previously so I am wondering if the problem comes from the PCR conditions. Indeed, Tm given by the calculator specific of this enzyme are much higher (sometimes 10° difference) than the Tm found in other papers. I called the technical support related to this and they advised me to only test the Tm given by the calculator and eventually 2 degrees less. One of my colleagues just advised me to go for 5°C less and that it might be the reason why I didn't see anything for 3 markers (Tm too high). Has anyone got experience with this enzyme and knows if that could be the problem ?
Second question, it seems that this enzyme is a bit too much for what I want to do after with the PCR products. I am currently looking for PCR enzymes less specific, but still good enough to have good results (error rate low enough). I saw the HotStar HiFidelity Polymerase Kit (QIAGEN) and the Phusion Hot Start II High fidelity (ThermoFisher). Has anyone any other suggestions ? (PCR product sizes : between 500 and 800 bp).
I would be very grateful if someone can help me or give me an advice.
I wish to examine laminin in fish fillets connective tissue, but I am not sure which type(s) would be most prudent to start with. Has anybody experience or knowledge about distribution of the various laminin types?
With the continuing growth of the aquaculture industry, more attention to fish welfare must be given as it has significant impacts on health and resistance to diseases.
I am compiling a catalogue of fish vertebrae and scales from the most important fishes off Peru (in terms of abundance). One of my colleagues gave me these fishes collected with multi nets (for zooplankton) off Peru and I have problems with the identification of some fishes.
My guess is:
m093_mn05_n1 sample2" correspond to Triphoturus oculeum;
m093_mn07_n2 sample1 correspond to Lampanyctus parvicauda,
m093_mn07_n2 sample2 correspond to juvenile individuals of Triphoturus oculeum
m093_mn05_n1 1of4 correspond to Diogenichthys laternatus.
Here are some details of the stations:
m093_mn05_n1: ±12 °S, 1000 to 600 meters depth
m093_mn07_n2: ±14 °S; 600 to 400 meters depth
Hope anybody could help!