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Hello
I am having a high-resolution daily data (~20 gb) with 0.25 deg horizontal resolution from 1998-2020. I am trying to compute the daily anomalies and I tried python, Ferret, NCL and CDO.
in Python using xarray it produced the output but it does not have anomalies for 29-Feb. Then I tried Ferret but it does not correctly account for the daily climatology because it subtracted 1-March from 29-Feb from a non-leap year same problem persists for the CDO.
At last I tried NCL but because of heavy data the NCL operation got killed due to machine limitation.
Is anyone having a code or process to do the daily anomalies correctly for heavy multi-year data containing leap years?
Cheers, Saurabh
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Use DataArray.dayofyear function, it'll take care of leap years
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Black-footed ferret (Mustela nigripes), a North American species presumed extinct in 1979 until it was rediscovered in 1981. Same thing happened to the Bermuda petrel, peccary, venomous Cuban Solenodon etc.
And the list is much bigger.
The main argument is that if there is no concrete reason, why the organisations are putting the term 'extinct' for a species?
Is it not controversial and funny?
Is it not time to rethink about this problem?
Please jot down your opinions here with any updates in this area.
Thanks in advance.
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It's a very interesting question because in many cases it doesn't seem justified to declare any species extinct if it's not found after conducting a few field surveys. It is clearly mentioned in the 'IUCN Red List categories and criteria, version 3.1, second edition' that "A taxon is Extinct when there is no reasonable doubt that the last individual has died. A taxon is presumed Extinct when exhaustive surveys in known and/or expected habitat, at appropriate times (diurnal, seasonal, annual), throughout its historic range have failed to record an individual. Surveys should be over a time frame appropriate to the taxon’s life cycle and life form."1 The significance of the criteria I see is that it motivates researchers to get involved in more thorough field surveys in order to get any clue about presumed extinct species. In many cases presumed extinct species were rediscovered after a gap of a few years, but it doesn't mean that the previous researcher failed to locate the plant due to less dedication, we know that the distribution of many species is extremely random and the geographical area is so vast, and in some cases inaccessible.
In my opinion, it's not controversial but yes the criteria should be more precise and after the field surveys which failed to record an individual, there should be a set time period after which species should be declared extinct. We also have to understand that we can't be everywhere and thus we miss the plant's population and it happens a lot. If we set a certain time period there are many chances that the researcher could find the species before getting extinct status.
Reference:
1. IUCN. (2012). IUCN Red List Categories and Criteria: Version 3.1. Second edition. Gland, Switzerland and Cambridge, UK: IUCN. iv + 32pp.
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Hello
G'day
I am looking for a solution to perform the cross-track analysis of a tropical cyclone. This means plotting certain parameters across the track of cyclones. This is having x-axis with the time evolution of 5 days prior to the genesis of the cyclone and goes up to the 20th day since its generation and Y-axis shows the distance from the left-hand side (i.e. -300 km or -3 degrees) to the right-hand side (i.e. 300 km or 3 degrees) of the cyclone track.
I hope it is a very frequent analysis usually done in the cyclone study. Looking forward to your technical help in developing the code in NCL/Matlab/Python/Ferret.
Cheers, Saurabh
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Tropycal is a Python package intended to simplify the process of retrieving and analyzing tropical cyclone data, both for past storms and in real time, and is geared towards the research and operational meteorology sectors. So, I am not sure what you mean by "cross-track" analysis, and while I do not use Tropycal, I kind of get the feeling that it would provide you the tools you need to analyze TCs. Attached is a best tracks plot of TCs in the SW Pacific for the 2020-21 season; it is color coded by wind strength. I attach this as an example of what I think a "cross-track" analysis product is. A second plot shows TC frequencies during a time period - that could be another such product. Frankly, I really don't know what you want to do, but somehow I think that the Tropycal package can probably assist you. If I am wrong, then my apologies, but again, you asked a question and I merely provided you with a possible solution. Good luck in your work.
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Suppose in a A1-xB2CxO4 spinel ferrite, someone found the average grain size as follows-
x=0.00 0.8 micro meter
x=0.10 0.1 micro meter
x=0.20 1.2 micro meter
x=0.30 1.4 micro meter
x=0.40 1.1 micro meter
x=0.50 1.5 micro meter
Here, the 4th composition didn't follow the systematic sequence. It was supposed to increase from the 3rd one. What reasons may have behind this phenomenon?
Regards
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I agree with Saurabh Singh about the importance of the synthesis method and the likelihood that the non-monotonous trend you saw was caused by experimental problems.
However, if you have convinced yourself - if possible by more than one method - that the experimental grain-size determination is precise enough for the purpose, a physical explanation for the discontinuity may still be possible. For a partially relevant example (though with one inflection point rather than the implied two in your case) see
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Combustion technique is one of the methods used to synthesis material. It is inexpensive than the solid-state reaction technique. It makes the grain size in nanoscale.
What are the other advantages that's why one should adopt combustion technique instead of the solid-state reaction technique?
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Rakibul Hassan please find out the articles attached herewith, i hope it will help you to understand about the matter.
  1. https://www.sciencedirect.com/topics/materials-science/combustion-synthesis
  2. https://www.epa.gov/smm/energy-recovery-combustion-municipal-solid-waste-msw
Jazakallah khairan
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Hi all,
I'm going to run Flow Cytometery on ferret PBMCs but can't find an Fc block for ferret PBMCs. Does that even exist? Do I need ferret-specific Fc blocking antibodies or can I use: https://www.bdbiosciences.com/us/applications/research/b-cell-research/surface-markers/mouse/purified-rat-anti-mouse-cd16cd32-mouse-bd-fc-block-24g2/p/553142 ? I've seen this used in 2 papers as an Fc block for ferret PBMC flow cytometry and want to make sure it will work fine or if there's a better alternative.
Thanks!
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Hu my Dear, I would recommend 1/10 dilution.
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Does anyone know where to find the sequence for the heavy/light chain constant domain of a ferret antibody?
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Good question
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The other day I heard two professionals comment on vaccine development in India. One of them said that animals like hamsters, ferrets etc. usually used in Vaccine research are not available in India. This poses a problem to those who wish to do vaccine development.
I wonder if they have considered using bats. Someone has reported finding SARS-Cov-2 in bats in India.
Even if they are not accepted for approved testing of new vaccines, they could be helpful in the process of vaccine development in earlier stages.
As a first step, we could test bats for specific antibodies.
Srinivasan Ramani
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Bat models of SARS-CoV-2 are not suitable for developing a vaccine, and have not been used anywhere in the the world to date.
A look at this link tells you why?
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hello
i am working on a Tropical cyclone intensification in Bay of Bengal
can anybody help me to plot a cyclone tracks using ferret
thank u
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Assuming your would like to identify the center of the tropical cyclone by its low pressure and assuming that you have a series of pressure organized as a netcdf-file, for instance, with three axis:
  1. x-direction (eg. longitude)
  2. y-direction (eg. latitude)
  3. time
So the first step could be to compute the temporal minimum pressure field
let press2Dmin = pressure[l=@min] ! minimum in time
Now lets compute for each time step the pressure difference to the 2dim minimal pressure field
let pressDiff = pressure - press2Dmin
You shall find the center of the tropical cyclone at the locations where the difference is zero
let pressLoc = pressDiff[l=@loc:0] ! Find in time/ L-index
I hope that it helps to answer your question.
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I cannot find any literature. Is it similar to the mouse? Rat? Human? Has anyone tried it as a model of retinopathy?
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Thank you! I just sent him an email. I appreciate the connection.
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I have yearly mean SST (3D: lon X lat X time) data for 100 years. Using this data I want to calculate yearly anomaly SST data. What is the procedure to do this in ferret?
Answer is expected: how to do it using "repeat" loop in ferret over time-steps and subtracting climatological mean from every time-steps. (Any other solution is also ok)
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Hello Subrota Halder,
To save yourself from stress.
Install "cdo" using
sudo apt-get install cdo =============> (on linux)
To convert monthly data to yearly data (assuming the name of the original monthly data is "SST_monthly.nc ")
cdo yearmean SST_monthly.nc SST_yearmean.nc (ignore this is your data is yearly already)
To calculate the long-term mean use:
cdo timmean SST_yearmean.nc SST_clim.nc
To calculate the yearly anomaly use:
cdo sub SST_yearmean.nc SST_clim.nc SST_anomaly
If you insist on using Ferret, use this example
Cheers.
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What possible damage mechanism rather than hydrogen em belittlement or HIC , if the secondary crack pass through ferrite of duplex only and principal crack is trans granular,
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Yes but no any source for H2Sin that process, we need to know about hydrogen embrittlement in duplex ,what is possible sources of H2 from manufacturer it could be ??
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I'm going to detect the Pandemic 2009 A(H1N1) Influenza Virus in nasal washes of Ferrets by qRT-PCR, so I don't know if it's necessary to put house keeping gene or not? if so, what are the best (house keeping gene)s used usually in nasal washes? thank you for the answers!
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Thank you for your answer Jack.
But could you please explain how you analyse and interpretate the results without housekeeping gene?
If we have infected nasal washes, non-infected, control positive pure (pdm 2009 H1N1), and negative control.
In this case we doesn't have delta Ct, in my opinion simply we can say that our samples (nasal washes) is infected (contain H1N1) or Not.!!
So, we couldn't able to measure the quantity of expressed RNA for the virus (H1N1) in nasal washes different samples.
Thanks for your reaction!
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Would like to know the procedure to collect ferret faces for measuring the microbiome. But I am not sure how to stimulate ferret to defecate.
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No need to anesthetize, but we do since we collect multiple samples (blood, respiratory) at the same time. can just use sterile swabs used for human collection of respiratory or throat samples.  can prewet swab with PBS first, which should make it easier to collect sample if necessary.  Generally have no problem collecting sufficient samples from healthy animals that are eating.  can e-mail me at txr2@cdc.gov and setup a call if you have other questions which I haven't answered here.
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We want to test the elevating of the serum cytokine (such as IL-1b, IL-6 and TNFa) level to confirm that injection of a virus into the ferrets will activate their immune responses. Is it appropriate to use mouse antibody for the purpose, or do we need ferret antibody?
Thanks. 
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Both have positives and negatives. It really depends on what you are looking for. If your target protein is either very small in molecular weight or there are very low levels of it in your sample then ELISA is probably better and slightly more quantitative.  Western blots can be tricky but are good for large, highly abundant proteins and can also be quantified using densitometry 
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I'm looking into investigating the different aspect of antiviral drugs in animals (especially mice and ferrets) co-infected with bacteria and virus. Just wondering if anyone has prior experience in terms of minimizing bacterial contamination between experiments (special ways of cleaning the cage) and the different precautions taken to protect personnel, animals etc. 
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All infections should take place inside a Biosafety Hood with proper PPE.  We routinely infect our mice by aerosols, depending on the virus and bacteria, this can be an option.  Multiple animals are infected in a plastic cage that is empty and sealed inside a BSL hood, aerosols containing virus or bacterial are fed inside the cage through plastic tubing. The animals are transferred into a clean cage after a few minutes has passed and the aerosols have waned. After, everything is sprayed with clidox/chlorox solution and 70% ethanol before removing from hood and allowed to soak in bleach for a few minutes before cleaning. 
If your antigens cannot be aerosolized (inactivation by aerosolizing) Intranasal inoculations are fairly safe to perform.  Most laboratory strain viruses and bacterial aren't transmitted by co-habitation and the animals constantly groom themselves so chances of transmission b/w animal and tech is rare, again it depends on the antigens.
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depending what you are looking for. there are several clinical parameters you can look at. You can contact me privately by e-mail txr2@cdc.gov and we can discuss other possible parameters.
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Can anyone suggest a model to find the estimation of long term extreme values of Significant wave height.-Trend values of Hs? I have the altimeter track data files.The models I have taken from research journals are POT, GPD and RHTestV3. I prefer to use Matlab or Octave commands.
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POT seems a good alternative.
Here is a tutorial (not mine) I've found that has a sample Matlab code
Hope it helps!
Andres
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I have extracted the nc data from Ifremer and I am plotting it with ferret and octave. I need to compare the nc data of AVISO wave data and Ifremer wave data. I am using ferret and Octave in ubuntu.
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Dear Sir,
I would like to get your help. I have mailed you. And sir I would like to know can I use Liveroms tool for plotting and interpolation of grid map for my project.
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A recent diagnosis caused me to question patholigists and veterinary specialists, none of whom had seen this in a ferret.
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We laughingly refer to any x-ray as ferret view because you get the entire body in the picture. I have had ferrets since 1993 and have been inside several. As Mr. Jekl said, it is a more central heart with everything else similar as to humans. I can provide more detail and am intending to publish this, but was initially just trying to find someone who had seen it. I actually have a vast number of exceptional ferret vets I can contact - and have - so next approached the research community. I do notice that heart problems are an issue in this condition and it was noted that the heart in this ferret is a bit rounded. The x-ray actually looked fantastic; I thought it was taken with him on his stomach instead of his back, or shown with the film reversed, but the vet had verified position and labeling before showing me. I own this ferret and he was seen for an unrelated matter. Thank you for your input. I will post more as I learn more.