Science topic
Felidae - Science topic
The cat family in the order CARNIVORA comprised of muscular, deep-chested terrestrial carnivores with a highly predatory lifestyle.
Questions related to Felidae
Hello everyone!
I want to analyze an open population of Amur leoprads using camera traps data.
Could you advise what is the best way to start the analysis of the methodology? Some basic and advance literature. I will be grateful for any help
I have been reading papers to determine the best way to evaluate dietary overlap for three small wild cat species (ocelots, jaguarundis and margays). In some papers about other species have used Pianka's index but I would like to know what other options would you recommend for this?
I want to prepare like following example figure for Indian fox diet data. But I don't how to prepare it. I have % relative frequency and % volume data. Does anybody help me how to prepare it?
The following example figure has taken from Lovari et al. (2013).
Lovari, S., Minder, I., Ferretti, F., Mucci, N., Randi, E., Pellizzi, B., 2013. Common and snow leopards share prey, but not habitats: competition avoidance by large predators? J. Zool. 291, 127–135.
Thanking you.
Himanshu

I am doing the age related methylation study of snow leopard. Since there is no commercial kit for me to use as control DNA, I made "fully methylated" by M.sssI with both blood DNA and its WGA product.
For blood DNA, I followed the protocol and incubated for 1hr. For WGA product, I tried 2 types: 1st, just incubating it for 4hr; 2nd, incubating it for 2hr and then purifying the product and replenishing reagents and incubating for another 2 hr.
However, according to the result I got from MS-HRM (see in the attachment), they should different relative signals which implied to different methylation rate (100% blood DNA 1hr > 100% WGA 2hr→purification→2hr > 100% WGA 4hr).
I am wandering whether it is because of the insufficient incubation time of WGA or the poor quality of WGA. I also want to know how to check whether the methyation rate really reached 100% or not. If not, how to improve it.
I am also considering to use synthesized 100% methylated DNA of target region if making 100% methylated DNA from M.sssI is too hard. Since the concentration of target region of synthesized short DNA strands must be higher than normal DNA, I think I need to adjust the input amount of synthesized DNA carefully before apply it. Does anyone has the experience, especially when using MS-HRM?

I have a reference:
Primer registro de Panthera leo atrox (Felidae, Mammalia) en el Pleistoceno de Hidalgo, centro de México. VIII Congreso Latinoamericano de Paleontología. XIII Congreso Mexicano de Paleontología. 23-27 septíembre 2013. Guanajuato, Méxíco.
Is it correct?
Thanks,
Gloria
What would be the best grid size for pres/abs survey of snow leopard using camera trapping in the Himalayas?
I'm studying interactions between North American predators, specifically wolves, coyotes and bobcats.
I've found evidence from game camera data I've collected that suggests there may be a significant interaction between wolves and bobcats. However, I haven't found published literature or reports on interactions between these two carnivores besides a paper by Stenlund (1955) that bobcats may benefit from scavenging wolf kills.
Does anyone know of any publications in peer-reviewed literature, government reports or even reliable observations from biologists in the field on interactions between wolves and bobcats? Reports on wolves and Canada lynx would be useful too.
Thank you!
Recently a cheetah was killed by a duiker at a study site in South Africa, and we want to know how frequently this occurs to all of Africa's large predators. We're looking for mortality events of lions, leopards, spotted hyaenas, cheetahs and wild dogs caused by prey species weighing less than 25 kg. Any evidence by way of publications or personal communications would be much appreciated.
Do tiger, leopard and clouded leopard have features of character displacement?
Please share contact details of a researchers/organizations working in Buxa Tiger reserve
I don't have permits to export to other countries.
Please contact me on [email address removed by admin] if you are interested.
We have recorded two separate incidents where a tiger killed jungle cats. We plan to research further on this topic but not sure if these are just two random killings or a topic worth looking seriously at?
I am proposing a study along similar lines, and so was wondering how often would I have to re-new the scents with fresh scent?