Questions related to Fat
A type 2 diabetic rat model was developed with High fat diet + low dose Streptozotocin. High fat diet included a 2:3 mixture of coconut oil & partially hydrogenated vegetable oil (10ml/ kg body weight per oral) IN ADDITION to normal pellet diet(56% carbohydrates; 18.02% protein; 3.95% fat; 7.85% ash, 8.75% moisture, 5.45% fibre, 1.6% Ca and 0·85% P, w/w)
How much % of total energy of diet is given by the fat in the HFD?
Is there perhaps a way to convert mg FAME/g fish performed on GC-FID to g lipid/100g fish? We had enough sample to analyse fatty acids but not enough to measure total fat. Unfortunately I do not have the retention factors of the equipment. Sample weight in 30mL CM was 50 mg. 10 000ul of this solution was transmethylated. We used 100ug C17 internal standard.
Any recommendations are welcome
I want to include Crude Fibre, Crude Protein, and Crude Fat analysis of my plant sample (pulverized). If any lab (Indian) can perform these without charging, I shall provide co-authorship in the relevant manuscript, which I intend to send to an SCI/SCOPUS indexed journal.
Thank you, and looking forward to your responses.
In partial hepatectomy models, fat accumulation occurs during hepatocyte regeneration. Whether it is needed is controversial. Though it was initially thought to be the "fuel", later experiments have shown that proliferation can proceed even if fat accumulation is disrupted. My question is, what triggers this accumulation and what is its function.
I am currently working on analyzing the association of dietary intake and microbiome with type 2 diabetes (T2D). I plan to validate my findings using publicly available databases and was wondering if there are such studies/cohorts that enrolled T2D cases and controls and have macronutrient (protein, fiber, carbohydrate, and fat) and/or gut microbiome data. It would also be great if the study focuses on an Asian population.
Has anyone developed or found specific anthropometric models for this population in the scientific literature?
I'm looking for this to solve a problem in my clinical practice..
I want to extract amino acids and nucleosides from samples with high salt/buffer, protein, fat and cellular debris. Amino acids and nucleosides will then be analyzed by LC-MS.
Do you know how I can isolate amino acids from my sample? And how I can isolate the nucleosides? (two different analyses).
We are modelling the digestive process so there is digested food stuff (blended before mixing with digestive enzymes) in the substrate that varies depending on the experiment and the substrate used.
The digested substrate is in 40 mL of liquid.
I would like to avoid MWCO filtration if possible.
We're trying to perform a finite element analysis (FEA) simulation on the application of force to the skin of a human limb. In order to set up the simulation we are in need of damping coefficients for two of the layers - skin and fat. We've searched for but cannot find separate values for those two tissue types. Can anyone point us to references for these?
Like I would like to get help with the steps in writing a practical report
Regarding the above question.......
Phosphorus applications in black cumin increase the seed oil rate. A similar situation is observed in other medicinal and aromatic plants.
I have been wondering why when using a model of high fat diet the control diet has to be the 10% fat instead of normal chow. Does anybody understand the rationale behind this?
We are starting soon an in vivo trial with broilers fed diets supplemented with microalgae and insect full fat larave. We will collect blood samples and I'd like to have your suggestions about the best parameters to be analysed on blood to evaluate the oxidative and metabolic status
When I set transesterification reaction with eggshell Cao and Beef fat/ palm oil, biodiesel was produced. But, When I set the reaction with the mixture of beef fat and palm oil (waste cooking oil or virgin palm oil), the reaction was never completed, I got only a homogeneous product of fat and oil only. even I have tried by varying the parameters like temperature (60, 70, 75), speed (500, 1100 rpm), reaction time (3, 4, 5, 5.30 hr), oil to methanol ratio (1:9, 1:12, 1:15, 1:20), catalyst loading (2.5%, 5%, 7.5%, 10%).
Please suggest to me what is the reason behind this and what would be the solution?
In my study on Awassi sheep, it was concluded that adding turmeric to the ration led to diverting the path of fat accumulation from the parts of the carcass to the broad tail, with significant weight increases in the treated lambs.
I am facing an issue in fish gut digestion using 30% H2O2. After the digestion process, I am getting a fat oil layer that might trap plastic particles. Kindly help me out with this to digest the oil layer or separate particles from it.
Post mortem sample.
Lungs, 100x, red-oil-O staining.
Regardless of the patient's history, would you consider the finding as a fat embolism?
I want to centrifuge yogurt in order to pellet bacteria and yeast cells. But of course all the fat pellets as well. I tried a mixture of EDTA and TE buffer as used in a journal article I found, but the fat solids do not collect on the top as indicated in the paper.
Could tri-sodium citrate be used to keep the solids from pelleting, and if so, what ratio would I use? Or is there another method that would allow separation of the solids without discarding the cells?
Do you think there is a correlation between diet and the spread of the SARS CoV-2?
Maybe a diet based on fish, rich in fat, omega-3 and vitamin D is better ?
Upon reading following paper: https://pubmed.ncbi.nlm.nih.gov/31727409/, I saw that the power analysis is based on a two-sample t-test, while the primary endpoint is based on ANCOVA analysis (with two groups: placebo vs treatment and baseline liver fat as a covariate).
Is it OK to perform a power analysis for a two-sample t-test while not using the same statisitical approach afterwards for the primary endpoint?
Secondly: is the p-value of 0.05 valid for the calculated power when there was an amendment of the protocol (two dosing groups became one dosing group)?
==> the authors write
"Around 117 patients, randomly assigned to 80 mg (two-thirds) or placebo (a third) treatments, and given an estimated treatment difference of 30% change in hepatic fat fraction from baseline to week 12 between any dose of resmetirom and the placebo group and a common SD for the percent change in hepatic fat fraction of 35%, would provide 90% power with a two-sample t test to achieve a significance of 0·025 for a two-dose multiplicity (after protocol amendment, the significance level was reset at 0·05 because a singledose group was used)."
Thanks in advance!
I want to extract lipid and protein from mice whole serum followed by quantification of Sialic acid in total fat. I found many methods but I am wondering, is there any kit available?
Hi, i was looking for some research papers on junk food advertising restrictions/bans in outdoor spaces to reduce the exposure to advertise of food and beverages that are high in fat, sugar and/or salt. AND Any pilot/project evaluations of implementation of advertising bans at regional and local levels?
I need a list of patents about the production of fat from cells.
Could anyone please help me?
Many thanks for considering my request.
I'm looking for informations about vitamins stability (both water&fat soluble).
I've done several HPLC analysis in different kind of dietary supplements (tablets, powder, liquid, ..) and I've found that, for example, Folic Acid is less stable in liquids than in powder (at same storage conditions). I'd like to know if, in your opinion, this is a common behaviour for this vitamin.
I'm also looking for the paper "A study of folic acid stability in solutions of the B complex vitamins" by Alfred R. Biamonte,George H. Schneller or similar.
I would like to cryosect aortic plus perivascular adipose tissue from obese mice. Unfortunately, at -23°C, with a new knife, embedded in Tissue Tek and very careful handling, the tissue is too unstable and it tears when cutting.
Thicker cuts have shown no improvement.
Do you have any tips on how to cut the tissue better?
Also maybe someone who has only cut fat tissue?
I am very glad for help:)
I have seen everywhere that mitochondria in brown fat produce heat as UCP1 diverts protons from ATP synthase. What I can’t find is whether these mitochondria synthesize any ATP at all. In other words, which part of the energy (and protons) goes through UCP1 and which through ATP synthase?
I will be grateful if any one could give a detailed description on their experience/precautions to be taken with RNA extraction from breast tissue (stored in -80). I am planning to use Qiagen RNeasy mini kit and homogenize the tissue with help of crushing in mortor and pestle with liquid nitrogen followed by homogenizing with lysis buffer. I was wondering the normal breast tissue which is usually with fat, will homogenize in the same way? Any information related to this will be greatly appreciated.
Hi everyone! I have had good luck asking questions before so I thought I should ask this. Has anyone else ever noticed that fat globules surrounding secretory tissue seem to prevent cells from emerging from the tissue? These are plated secretory tissue from bovine mammary glands. Fibroblasts and mammary epithelial cells emerge from tissue but I've noticed that there are none where there is a sort of "halo" of milk fat globules. Not sure if this is just a coincidence or maybe I just don't notice them as much when there are cells there.
I have the milk fat "halo" pictured and then a picture of emerging cells on another piece of tissue. Thank you.
I am working on the differentiation of MSCs to adipocytes . I would like to know if the fat droplet-containing cells are terminally differentiated adipocytes or are they preadipocytes? are these cells post-mitotic?
Hello, I'm trying to analyze the proteome of some tissue by mass spectrometry. After lysing the samples and centrifuging them, sometimes there is quite a bit of fat floating on top of the supernatant.
Even assuming that I have enough material to take only the "clean" part, I wonder: should I? Or should I rather leave it all there and hope that it gets discarded when I precipitate the proteins (e.g. Acetone)?
I'm thinking that I might be losing lipophylic proteins (e.g. membrane transporters) if I remove the part of the supernatant which floats on top. But I also don't want to risk having fat messing around with ...anything. How do you go about it?
Thanks in advance.
I am debating the best way to normalize indirect calorimetry data when comparing normal mice (about 30g) vs obese mice (about 60g) Using body weight is not correct since fat has a very low energy expenditure (EE) so obese mice would present a lower EE/g than the real value. On the other hand, I consider that normalizing the data only with the lean mass is also incorrect. Normal mice present about 10-15% of fat and obese mice around 50%, I think it would be a mistake to consider that half of the body weight would not participate in the energy expenditure. There is the option of showing EE data without controlling for the animal weight, but bigger mice would have higher EE. I was looking for a formula combining lean and fat mass for data normalization but I could not find it. Have you heard about any consideration about normalizing indirect calorimetry data when comparing normal/lean mice vs obese mice?
Thank you very much.
I am Performing the pomegranate juice evaluation, So Please help me with finding lipid or fat percentage or provide the link of a related paper or website.
Dear all experts,
I has calculated the fat oxidation during exercise every 5 minute till 60 minutes, and found the negative fat oxidation when the RER is reached up more than 1.00 (using the Perronet's equation). How should I do with the negative result? Should I cut that result before calculating the total fat oxidation or calculate the total fat oxidation by using all results which are positive and negative?
I'm required to do a histology experiment to determine fat accumulation after an HFD and to compare it to a normal diet. I planned to utilize red O oil staining, however, the equipment for this experiment was not available at my university. Besides, I am unable to go to other campuses owing to the lockdown, which seems to be taking an extended period of time. Thus, I wonder if H and E may substitute oil red O stains
I am stuck in the result analysis of the physiology lab report. I am looking for the effects of ketone ingestion on fat oxidation rate. The same participant was tested before and after the ketone drink ingestion and measurements were taken at 4-time points. which test could be used to see the effects of ketone ingestion on fats oxidation rate.
Does anyone have experience in extracting proteins from rat white adipose tissue?
I would like to analyse them in Western Blottingand I couldn't remove all fat cake from samples.
What percentage of trypsin should be used for mouse fat mesenchymal cells? These cells are difficult to separate from the bottom of the plate. What can I do to increase the growth of my cells in the flask?
Are fat mesenchymal cells divided into two groups?
The results of my study are leaving me a little baffled. I would appreciate any insight you could give me.
What I expect: Larger objects (living or non-living) should heat up slower than smaller objects
My results: Heating rate is increasing with size, larger beetles heat up faster than smaller beetles
I tested for: The amount of water absorbed during the rehydration process does not play a significant role.
I am wondering: Could it be related to differences in fat/protein contents?
β oxidation requires sufficient oxygen when glycerol is converted into dihydroxyacetone acid, which enters the sugar metabolism process; fatty acids are converted into fatty acyl-CoA, which enters the tricarboxylic acid cycle for complete oxidation. But now we try to envision that E. coli can use fat in the intestines.
As part of my master's degree, I am working on a research project to cultivate fat lobules from explant tissue. We would like to study cell viability and functionality of fat cells over a period of 14 days in vitro and are looking for easily feasible light microscopy methods that are not as labor intensive as the preparation of sections.
I am looking forward to your answers!
I am working with a diet induced obese model and run my mice on indirect calorimetry cages. I am search for the best method to adjust the energy expenditure (EE) results but there are some considerations to have in account.
It is assumed that fat does not importantly impact EE and people usually adjust EE to the lean mass (NMR based). However, my high fat diet (HFD) mice have 3 time more fat that the low fat diet (LFD) mice, and with it probably more brown fat also, that is very active metabolically. In this study is not completely correct to adjust neither by total body weight nor by lean mass, and absolute EE might be harder to analyze since BW differ a lot between LFD and HFD.
Is there any combined formula that is accepted to use? If so, can you reference the work?
Thank you very much.
Andre Cabral, PhD
Fish specimens fixed in formalin are stored in 70% Isopropyl alcohol and 1% Glycerin solution. After some time, a fat layer was observed on the surface of the container. But when they were stored in formalin this never occurred. What might cause this effect?
I used to extract RNA from epididymal fat（Using Takara RNA extract kit），approximately 900 mg epididymal fat tissue can get ideal RNA concentration. While the weight of brown adipose tissue of a C57/BL6J mouse is about 60 mg. So how can I extract RNA from brown adipose tissue (BAT) for qPCR?
Can the consumption of extra fat (2%) in the diet of prepubertal gilts cause an increase only in the weight of the ovaries? The rest of the uterine structures are not affected (no CL, CA..)
Why does this occur?
I am working on mouse adipose mesanchymal stem cells. After removing the mouse peritoneal fat and extracting, I do the primary culture, I used DMEM F12 medium with 15% FBS, but with this, the growth rate of these cells is not good and they seem to grow slowly.what am I suppose to do?
How much metabolizable energy is need to producing a one-kilogram broiler and poult live weight with the lowest fat carcass?
How to calculate the energy requirement for a special purpose of weight and carcass increasing body weight in different weights?
Are there any differences between breast and thigh energy requirements for increasing weight in broilers and poult?
Is exist a preference for particular nutrients in the breast and thigh in the broiler and poult?
I am studying Treg using a mouse model. In order to get a high number of splenic and thymic Treg, I combine 10 spleens and thymus and digest both tissues using collagenase type 4 for 30 min. After digestion, tissues are filtered using 70 um and 40 um strainer and centrifuged to get a single-cell suspension. And, I found a huge amount of fat or mucous-like materials in the pellet. I tried to remove this by filtering again, but it didn't work well. Downstream procedures are affected negatively, leading to failure of enrichment of mouse CD4+CD25+ cells. Actually, I can carefully get rid of the fat or mucus using the pipette and blue tip.
Have you guys ever happened to this issue and solve this?
I’m doing a meta-analysis on several continuous variables (fruits, vegetables, whole grains, fat, and so on) of the same outcome (dietary intake).
Can I put all the different variables in the same forest plot (as if they were subgroups)?
Thanks so much in advance
For evidence, a few snapshots from any Scientific/Medical Conference are enough.
Physicians are obviously not good enough to heal themselves, and, do not practice what they preach.
We routinely use the iDISCO+ and Adipoclear techniques followed by imaging on a light sheet system and I find very bright punctate labeling around the outside of tissues such as brain or fat as well as along the inside surfaces of ventricles. We filter our secondary antibodies through a 0.22 um filter as described in the protocol but that does not seem to be enough. Does anyone else have this problem or any suggestions that we can try and reduce it?
Was wondering if any of you conducted an Oil Red on AML-12 cells, if so, after how much time did you executed this?
Some studies call for 24hr and some for 18hr.
For fat content analysis.
When a person is put on a diet consists of low carbohydrate and high-fat, the body starts using ketones from fat for energy instead of glucose. This situation forces the body to use fat stores as fuel, which may lead to weight loss. Acceptable macronutrient distribution ranges (AMDR) are 45-65% calories from carbohydrate, 20-35% from fat, 10-35% from protein. What could be the long term effect on overall health if a person is put on low carb and high fat diet?
We are doing research with C57BL/6 mice to induce obesity using High-fat diet (D12492-60% kcal% fat).
After 2-week observation, the high-fat diet intake mice have a wavy hair problem and also sample treatment group showed the same. We are curious without any sample treatment mice also having a wavy hair problem.
Please help me out! Is there any problem with the high-fat diet or not?
I have a dietary intervention experiments on diet-induced obesity animal model and the results demonstrated that the intervention reduced body weight and fat mass and increased lean mass with statistical significance.
Although weight loss ≥ 5% is considered meaningful, what percentage changes in fat mass and lean mass is known as clinically meaningful?
If you have answer for this question, please also provide the reference so that I can cite it in my paper.
I want to apply regression analysis using SPSS. Kindly help as to how to apply the regression model in the study where i have recorded Weight, BMI, Total body fat mass, insulin resistance and nerve conduction velocity in type 2 diabetics.
I want to do scoring for macrovesicles and microvesiclular fat diposits in Histopathological Liver sections. Can anyone suggest me a software and discuss a suitable protocol to achieve this. I want to check for the development of Hepatic steatosis or fatty liver condition.
WHO recommends a fat intake of no less than 15% of the daily caloric requirements (https://www.who.int/dietphysicalactivity/publications/trs916/en/).
However, imagine a catastrophic situation in which a population somehow lost access to fat-rich foods for several years, while still having access to a good supply of carbohydrates, protein and micronutrients. Is there a point at which nutritional problems would arise if the consumption of fat was near zero? Would people survive if this was the case? what if the consumption was 5% or 10% of the total caloric intake?
Could the body survive via its own capacity to synthesize fats from carbohydrates?
-Please assume that essential fatty acid consumption (linoleic and alpha linoleic) is covered in this scenario-
We are trying to extract RNA from liver samples that came from animals that were either fed a high fat diet or a low fat diet (Using Qiagen mini kits). Should we use a lipid mini kit for both liver samples, a lipid mini kit for the high fat diet livers and a regular mini kit for the low fat diet livers or does it not really matter?
I'm trying to compare several different surfactant efficiencies for Swage Fato Oil and Grease emulsification into water. I don't think that methods for fat determination in food for example Ethanol extraction after the emulsification won't really work in this case due all sords of different impurities into the FOG material.
Maybe you have faced similar problems.
hi, i want to ask something about weight.
i provide a high fat diet(around 45% of fat) to my mice and did a gavage sth( one of herbal thing)
but one group of mice eat lesser than other but gain more weight.
Concentrate what i did a gavage was less density,
the other group is more higher density, but wasn't like that.
even the group only provide high fat diet ate more but gain weight less.
is it possible eat little amount of high fat diet and gain a weight more than other mice group i describe?
Have any way to gain a weight in healthy way while they take high fat diet?
I am dealing with an internal combustion engine, <500kW rated power, 1500rpm rated speed, for stationary application in CHP configuration. The engine, designed to operate with neat vegetable oil, operates a Diesel thermodynamic cycle and it is directly fuelled with animal fat. An excessive amount of Calcium (Ca) was detected through a chemical analysis after several failures occurred. In particular, fuel filters clogged several times, and I am wandering if the excess of Ca could have caused those failures. In particular, I am curious about the possible precipitation of Ca-based solid particles, also combined with a wrong thermal management of such a viscous and chemically unstable fuel.
Hi... Currently I am developing RTD milk coffee. As I read on article, the low molecular weight surfactant can replace protein from the surface of fat droplet. Indeed it can reduce the fat droplet size better than protein in the beginning, but the stability of the emulsion will be less during storage. Then this several question arose in my mind:
1. if we have enough protein in the system, does it mean that the additional LMW emulsifier is not necessary?
2. In case there is no enough protein, does the addition of protein (such is in the form of sodium caseinate) is a better choice than LMW emulsifier?
Thank you in advance for your answer! :)
We are testing various physical parameters of both fish and tomato samples, such as acidity, color, texture, fat content, etc. What is an appropriate sample size for these types of tests? References are always appreciated.
I am Ruminant Nutritionist, we bought commercial calcium salts of fatty acid (By-Pass Fat), we tried to analyze the fat contents by soxhlet method, it gives less than 3%, Actually, it contains 84% fat
I am trying to test my scheduling algorithm in cloudsim. However I am trying to create fat tree data center that consists of computing servers, storage servers and switches. Any help regarding how to start creating this topology is appreciated.
I am using Ethanol 95% for fat extraction from Soxhlet & sometimes extraction comes up and sometimes not with same setup. The water boils properly but the solvent is not actually boiling but the vapor seems come down. What could be the issues? Can anyone help me with the solution?
Thanks in advance.