Falciparum Malaria - Science topic

There are incidences of Falciparum malaria cases in my working place who have been de-inducted recently from South Sudan. According to the patients they were using Mefloquine prophylaxis weekly , However discontinued after de-induction(ideally should be consumed for 4 weeks after de-induction). What is the effectiveness of chemoprophylaxis and its mechanism

What should be an ideal way ahead? , besides active fever surveillance and health education about continuing prophylaxis and other personal protective measures like barrier clothing , use of repellents and mosquito nets

How long does it last? Can it prevent attacks altogether?

I am working on a research to determine the endemicity classes of different regions in a country which means that each region has to have one endemicity class. I am looking for ways of using different classes for each region to come up with my results

Hi there!! I want to do quality check some Sanger sequence reads and realized that the reads contain some odd letters (N, K, Y, B etc) different from the normal 4 DNA base letters (ATGC). What can i do to edit away these strange nucleotides represented by the strange letters?

I work in a non endemic region of India. I have diagnosed many patients of malaria presenting with fever after travel to endemic regions using these kits.

Malaria antigen test kits had become a reliable tool for ruling out falciparum malaria among tourists with pyrexia and seizures.

An expert committee recommended banning the kits to curb increasing malaria cases in endemic regions.

Govt of India has now banned entire production and sale of these kits in India. People in non endemic regions are not now well trained in slide and microscopy methods. Sudden ban of kits can also be counterproductive.

Do you think that this was a wise step?

What was your experience with these kits?

Definitive host allows the parasite to reproduce sexually. Plasmodium sexually reproduce in the mosquito, then why it's an intermediate host?

how can i isolate plasmodium spp. from whole blood?

Case 1: When treating a P.falciaprum patient, we consider that treatment failure is due to recrudescence or new infections. The new infection may already be present in the patient in a hepatic form.

Case 2: When malaria control efforts reach the stage that elimination is being thought of, major mass drug campaigns may be considered. Mass campaigns conducted with drugs killing the blood stage will, unfortunately, not eliminate the hepatic stages. Even with several campaigns with virtually 100% coverage, there may still be 'one man' (P.falciparum) somewhere in the liver.

(I ignored the mosquito stages for simplicity)

I have some other reasonings but I think the question is clear.

24 year pregnant lady presented with high grade fever with altered sensorium, diagnosed to be a case of complicated falcipaum malaria, but with very high level of uric acid (17.4 mg/dl), she responded to Inj Artisunate plus Clindamycin and her uric acid level also gradually becoming normal without any hypouricemic drugs. A second patient with long standing history of gouty arthritis with multiple tophi presented with acute severe pain and high grade fever, malarial test was positive and he responded to antimalarial and there also respond to his arthritis (he was also on colchicine)....now, my question is whether antimalarial has any effect on hyperuricemia?

Hello All, 

I am trying/order to find Plamion, Plasmagel, or Gelofusine (modified fluid gelatins or plasma substitutes) for a Plasmodium synchronization experiment. Anyone have any ideas as to vendors of this item here in the U.S.A.?

Thank you!!!

I am presently undertaking a study on markers of resistance to antimalarials in my locality. I want to exchange some ideas with any researcher who is presently doing or has done work on this. Thanks

Hello, I am planning to carry out an experiment involving Plasmodium falciparum infected Anopheles mosquito. For this, I have to artificially infect the mosquito with human blood containing gametocytes, after collecting the blood in to heparinized tube. So, in what condition should i keep the sample to prevent exflagellation of the microgametes for successful infection of the mosquitoes and for how long can I keep it before carrying out the experimental infection? 

I am carrying out a research project to investigate the dominant circulating variants of the pfAMA1 gene in a population. I have already extracted the plasmodium falciparum gene but need to carry out PCR and sequencing to identify frequency of the different variants. I however need to know the region which exhibits the 3 variants and the length in base pairs so as to perform nested PCR and also determine the cost of sequencing according to the number of base pairs thus preventing wastage

I am isolating Plasmodium falciparum merozoite from 3D7 culture, I am doing sorbitol synchronisation and enrichment through MACS column, followed by maturation of parasite in mature schizont, Than I am passing mature schizont through 1.2 um syringe filter. Every steps are going fine, I am able to get merozoite at the end but it is in ~1 ml volume which is higher enough I want to concentrate in 50-100 ul volume. I tried multiple time to do this by spinning at different speed (up to 10000 rpm) but not successful and I lost larger proportion of merozoite by this process. Can any one share their protocols, even if it is different method. Or if there is optimised protocol that can be used to concentrate merozoite.

In RPMI 1640 medium for Plasmodium falciparum, we add Albumax II as a serum replacement.

This information is important to time when to synchronize my culture.

I read so many reports where it is mentioned that sometimes it happen that plasmodium is left in the liver without being affected by the drug regimen and then suddenly it reappears.

I was wondering whether it is feasible to segregate live micro-and-macro-gametocytes of Plasmodium falciparum.

I am working on Plasmodium falciparum, I cannot get the media - RPMI 1640. How can I prepare it locally?