Equidae - Science topic
Equidae is a family of hoofed MAMMALS consisting of HORSES, donkeys, and zebras. Members of this family are strict herbivores and can be classified as either browsers or grazers depending on how they feed.
Questions related to Equidae
I have been researching on bullet proof military technology and wanted to asses the vulnerability of human body on the battlefield. I would love an layout and explanation of this question.
In my imaging of mouse brains, there are fluorescent dots that appear in the image from confocal microscopy which are not any of the target cells in my experiment. I was wondering if using a secondary antibody from a donkey and a normal donkey serum in the blocking could potentially cause this?
I am working towards to design a study to asses the efficacy of different LRRK2 inhibitor compounds in neuron. I have been meaning to ask if there is any gold standard to check LRRK2 enzymatic activity.
I know that PSQI is used as a self reporting questionnaire to evaluate and score the sleep quality of a person. I am currently trying to test the effects of different conditions on sleep quality.
My question is: Can I use this questionnaire each day where participants will be filling it after waking up from a specific condition night? Conditions I am testing is separated by nights and will continue for 3 to 4 weeks with random order.
I am looking for a measurement tool for to asses antisocial behavior in university students and i did not find any good measurement tool plz if someone know plz let me know as soon as possible
Do we need exploit further the genetic robustness aroused from distant hybridization? Mule is an excellent example in this regard.
I did ApoTox-Glo Triplex Assay to asses viability/cytotoxicity and apoptosis on three prostate cancer cell lines (LNCaP, 22Rv1 an PC-3) stimulated with high concentration of docetaxel and campthotecin in my pilot study.
I observed no differnece between control and research sample, moreover cytoxicity was higher in control (LNCaP)
Could I ask for any suggestion what to improve? Did anyone work on this lines? Could you reccomend an alternative for ApoTox?
I'm working on my research proposal about Epistemological belief and I need help in finding an instrument that could asses students' epistemological belief specifically in physics. Your help will be greatly appreciated
A group of my students would like to asses the effects of video games, play station, mobile games etc on sleep quality in medical students. Hence, they are looking for a validated questionnaire that would facilitate their project.
What is your experience in using runoff models in small gaged and ungaged catchments (less than 50 km2)? How did you asses the accuracy of the model you used? What was the input data used in the process?
I am comparing the activity patterns of zebra and wildebeest in order to see which animal is more active, and which forages for longer over a 24-hour cycle using camera trap images. Since the population sizes differ vastly, comparing counts won't help, and I'm concerned about compounding errors associated with standardising the sample sizes. Another option I considered was comparing the proportion of images collected in which foraging behaviour was observed, with the proportion of images in which all activities other than foraging were observed. I am worried however, that given foraging is typically a "slow" behaviour relative to running and walking for instance, and the speed of these activities differ interspecifically, that it would be underrepresented and wouldn't allow for interspecific comparisons to be made.
Thank you so much,
my project is to asses the birth preparedness and complication readiness among pregnant attending antenatal clinic in West Malaysia.
i have emailed most of the author of the related study but non of them answering my question and non of them willing to share those questionnaires
Planning to use an intervention. I am trying to understand the way we can take feedbacks from the field experts about the efficiency, appropriateness, and relevance of a particular intervention.
I'm currently experiencing more diverse signs for Rabies virus in animals-particularly in domestic dogs and donkeys. But when we send samples to the laboratories, the results are more often positive.
Can anyone help me with the rationale behind this, please?
i am planning to do my research on attachment and instant digital gratification among adolescents. if any of you have some materials related to it or scales kindly pass on to me and help me in this process. i appreciate in advance your generous heart.
I am doing Love wave tomography for the region of India and Tibet. Curious to know is there any method or way to solve the smearing in the tomographic checkerboard test.
I am trying to use STATA to create a funnel plot to asses publication bias using proportions from studies for a large meta-analysis (having used the metaprop command).
I"m having some trouble formatting the funnel plot to give me a meaningful result.
Any help would be much appreciated! Thank you!
As the tittle says... Since tamoxifen induces DNA recombination, is it possible to detect this recombination using specific primers? I am also assessing recombination through immunohistochemistry, but I was looking for other methods.
My subject animal is the zebra finch, with which I can typically only obtain small volumes of blood (resulting in only about 20 to 40 uL of plasma). The kit I am using (Enzo Life Sciences) has a small volume protocol that requires 10 uL of plasma. I used this and found that baseline corticosterone levels were undetectable. There is nothing wrong with the kit because all of the standards and controls are fine, and the kit detects stressed-state concentrations.
How can I increase sensitivity without extending the standard curve or using more sample volume? Does anyone have experience keeping the plasma samples chilled while the rest of the kit is room temperature so the steroid displacement reagent can work more effectively to increase free CORT? Would longer incubation with the plate-bound antibodies work?
Hi, I am a student from the Bocconi University, I have decided to write an Emphirical Thesis on smartwatch. I am interested in actual smartwatch users, but I have a very unsolvable doubt: can the Technology Acceptance Model be applied to actual users of any technology or the sample population must include also potential users (i.e also people that currently do not own or use a smarwatch)?
In the actual definition of the TAM said that "technology acceptance model (TAM) is an information systems theory that models how users come to accept and use a technology" but I have not yet found a research applying the TAM on an actual users, even if the definition stated that can be used for asses the use of the technology.
I am interested in using the Postpartum Partner Support Scale by Dr Cindy Lee Dennis in my final academic research project. If anyone can please guide me or help me about whether we require permission to use the scale, or anyone can suggest me any scale to asses Partner Support related to Postpartum Depression Thank You!
I'm working on a retrospective study to asses the QOL in adhered rheumatoid arthritis patients, I didn't have any issues with measuring the adherence using PDC Retrospectively but im planning on assessing the Quality of life prospectively by giving the patients questioners in their next follow up , any ideas on how to do that and is it a correct way or there are other preferred methods.
also is there a way on how can I measure the QOL retrospectively ?
I want to demonstrate that there is grow of primary follicle to a secondary stage in vitro not only with histological analysis but also with qPCR, any ideas on which genes could I analyse that have different expression in primary and secondary follicles?
Using formalin fixed brain sections. Sections are 50μm thick and fixed on the slide.
Was using 1 hour antigen retrieval in Citrate Buffer (ready made, sigma one) at 90C temperature.
Then washing 3 times for 10 minutes with 1% PBS-Triton-X100 and blocking (for 1 hour) with in the same PBS Triton with serum of Goat and Donkey, each 0.5%. I am not washing the sections after blocking. Just throwing out the blocker and then diluting the primary antibody in the same blocker.
Then Primary antibody (1:1000) overnight at 4C and then washing with PBS-Tween-20 (1%) and making of secondary antibody (Alexa Fluor) (1:1000) in PBS-Tween20 (1%). after the secondary antibody, washing with PBS. Using VectaShield as mounting medium.
Please tell me:
1. Should I increase the antigen retrieval time or temperature? or both?
2. Should I change the blocker? I am using 0.5% Donkey and 0.5% Goat serum in 1% PBS-Triton X100
2. Should I increase the PBS-Triton-X100 concentration from 1% ?
3. Should I increase the PBS-Tween-20 concentration from 1% ?
4. Should I change the mounting medium?
5. Should I increase the washing time. Now I am washing thrice for 10 minutes after each step.
should I change the whole protocol ?
I was offered to become a part of this journal editorial board - and encouraged to publish in it
I am suspicions.... you have to pay a substantial fee for publishing , the journal does not appear in SCIFINDER
If you can help me to asses this journal - I will be grateful
I am trying to asses the viability of stem cells in GelMA (gelatin methacryloyl). Most articles make their own GelMA. However, our team bought a commercially available one. The thing is : the product is fluorescent-green under the microscope, so it is difficult to evaluate the cell viability with the available staining kits, which show live cells also in green.
Experimental and comparative studies suggest that the striped coats of zebras can prevent biting fly attacks. Is there a practice in meat industry that can be fusion with the zebra striping taking advantage of both techniques?
I would like to do an immunostaining on mouse brain. I have a primary raised in goat and an one raised in rabbit. For secondaries I have a donkey anti goat and a goat anti rabbit (I do not a have a donkey anti rabbit). Is it possibile to do a serial incubation for the secondary (first the donkey anti- goat; wash, block, then the goat anti rabbit)? Will this prevent the cross- reactivity of the donkey anti goat to bind to the secondary goat anti rabbit?
Thanks a lot for the help!
I would like to asses the psychological distress among August 2018 flood-affected individuals.
Can I use GHQ-12 in 2020?. Because there is a time gap of 1 and half years after the floods, does it give an accurate result?
Currently working on ASS affected Ramsar Wetlands.
I'm particularly interested in the effects of sediment on migratory wading shorebirds, and on the invertebrates that lay eggs into the sediment.
What are invertebrate eggs called, how long can they persist in sediment, and does any particular geochemistry effect the integrity of the egg's shell? Or is it only the acid produced by ASS that's an issue? And if so, is there research that quantifies the effects of the acid on the invertebrates, or their eggs?
Do wading birds get sores from acidic pore water on mudflats? Does the geochemistry of sediment affect them in any way, at any stage, or is it simply the threat of ASS producing acidic water?
suggest alternative cell lines for HaCaT cell lines for the assessment of antipsoriatic activity
Dear Corrosion Experts,
Is there any research paper comparing various stainless steels ASS, DSS, MSS at different pH, Cl- ion concentration and Temperature? please share if someone has it.
I am writing this to gather some suggestions for my thesis topic.
I am a student of MSc Quantitative Finance. I am in need of some suggestions from the experienced members for a research topic in Portfolio management.
My expertise are in statistics and empirical analysis. I believe that I will be able to present some good work in field portfolio analysis. Currently, I am researching for some good topics where I can apply machine learning or machine intelligence e.g. for forecasting portfolio performance or may be use it to asses portfolio optimization strategies.
I will be very grateful for you suggestions and guidance. If it suits you, you can also email me on email@example.com
Assesing interaction by use of Parflow alone can lead to appropriate result?
My cells are cultured primary neuron from fetal rat, transfected with GFP-HA-double tag surface protein at DIV 7.
IF was preformed at DIV10-12, fixation with 4% PFA 15min and block with 10% Donkey serum. 594 fluorenes marked HA tag.
below is my picture captured with 60X microscope. The first picture is kind of OK but most of the rest is quite ugly. I am new to this and want to know what happend, would you please help me figure out?
The sections are about to mount. As far as I know leaving them too long in PBS (can correspond to too extensive washing) might weaken the signal.
(I have Dylight 488 goat anti-rabbit and Cy3 donkey anti-mouse sec. antibodies.)
I am looking for a way to asses animal welfare in a zoological institution on a longer term. I was wondering if anyone has used AWAG before, and what their experiences were?
To all scholars.
your inputs are welcome
1. How to identify a worthwhile opportunity and asses the identified opportunity using the PESTEL AND Porter’s five force model?
2. How to position the opportunity in the Ansoff’s opportunity matrix. decide growth paths from the initial quadrant to other quadrants when the company capture more opportunities.
3. How a company benefits from the BCG Matrix. What would you advise to a company which occupies a weak position in the “Dog” quadrant? What would be the generic strategy for a company in “Star” quadrant??
Will using Bovine serum albumin(BSA) as a blocking and dilution agent with donkey anti-sheep secondary antibody yield significant background?
Hi Flow Folks,
I've got a puzzle I need your help with. I've developed a flow assay to stain for intracellular neuronal markers. The protocol uses either 2%PFA fix with 0.1% Triton X-100 perm or the Thermo eBioscience FOXP3 kit. With either kit I also add 10% donkey serum to the perm/wash staining buffer. I also maintain the perm condition through the primary and secondary stains since I'm using indirect staining methods. I've used this method to titrate a total of 6 unique intracellular antibodies. I have good staining index for these markers.
Here's where it get interesting. To test the antibody specificity I want to use negative control cells that do not express the markers of interest. Using Human Protein Atlas I selected BJ, PC-3 and Reh cell lines. However, when I run this protocol I get positive staining on my negative cells! This has me quite puzzled because we have validated these antibodies for use in ICC and know that they localize correctly. Take a look at the attached image for one marker (MAFB) to see what I'm talking about. Secondary only and also isotype plus secondary give negative staining so I don't believe this is just simply blocking. What do you think?
To asses the success of of prepared adsorbent what isotherm it should follow? I mean there are lot of isotherm studies describing different methods of adsorption out of them which is considered as the best so a to manufacture an adsorbent commercially?
i have a set of users that are located in different Ass (Autonomus System) and I want to find for a target user list of thier neighboor (closed location ) , i find works that say As -level topology has been widely used to measure distance between internet users , i want to have more information like how to measure distance between two As ?
Mice were perfused with PBS and 4% PFA
we use a rabbit anti mouse ASB4 AB in PBT buffer with 5% normal DS on at 4°C followed by a 2h RT incubation with donkey anti rabbit Alexa fluor 568
wash steps are with plain PBS 3 x 10'
even in our negative control (only stained with secondary AB) we get a slight background film which makes it hard to see the true positive cells.
Any ideas what could be the reason for this?
I've added a similar protocol in attachment
I am looking to do some simulation on liquid crystal. What I am trying to simulate is a system like this. Let say I have an electrode which on top of it has a polymer layer that works ass an aligment layer for the liquid crytals on top. How would the direction of the liquid crystals would change as I change the voltage applied to the electrode.
Hi everyone. I am interested in asses biological activity of purified reelin in cultures, so I have decided to analyze phosphorylation of Dab1 by immunoprecipitation and WB against p-Tyr residues, as other authors have done. Could anyone recommend me any good antibody for immunoprecipitation of Dab1? Thank you so much
For the past few months, I've been trying to knockdown a gene in HEK293 cells using siRNA. I've tried different transfection reagents and have recently started using RNAiMAX as I had heard that it's very effective.
I have noticed that it kills my cells and that when I'm doing RNA extraction, the RNA concentration of samples that contained Lipofectamine (even the mock samples without siRNA) have very low RNA concentrations. I then do qPCR to asses knock-down efficiency but I don't know how trustworthy the qPCR results are given the quality of the cells.
I'm using a 24 well-plate and cells are about 50-60% confluent at the time of transfection. I create a mastermix of Lipofectamine and OPTI-MEM as instructed. In the end, each well contains about 1 μl of Lipofectamine. Is this too much? I used to use more than that but since it's been killing my cells I've been gradually decreasing the concentration but still no success.
Does anyone have a successful protocol for mammalian cell line siRNA transfection using Lipofectamine? Any help will be greatly appreciated. Thank you.
in a cross of male horse with a female donkey fertilization takes place inspite of different genomes, but the offspring's produced are sterile.
In order to asses muscle performance in MDX mice, I have performed the four limb hanging wire test. According to the literature this test should be able to discriminate between MDX-mice and C57/Bl10ScSnJ mice. However the majority of C57/Bl10ScSnJ mice deliberately jump off the grid. None of these mice were able to hang for 600 seconds, however they are able to hang for 100 seconds. Maximum hanging times of C57/Bl10ScSnJ are very similar to those of MDX mice. I have followed the protocol that is described in SOP's.
I have tested 4, 6, 8, 10, and 12 week old mice. During my first set-up mice hung at a height of +/- 27 cm. In order to improve the hanging time, I have increased the height untill +/- 40 cm, however this does not seem to have a lot of effect on the max. hanging time... Any recommendations? Should I increase the height even further, however I do not want them to harm themselves.
I found that to asses effect of intervention we usually use one group pretest posttest design. but that design mainly uses primary data while I'm in a position where I can only use secondary data
I will isolate different fungi and see if they produce any metabolites with antibacterial properties against E.coli and Staphylococcus aureus. I want to use 3 different media and grow the culture in both solid and liquid substrate.
I have found mixed nematode infections in donkeys sampled from a local community in northeastern Nigeria. I am looking for the best way to explain my findings in terms of epidemiological significance.
Suppose we have time series variable X1, X2 and Y1. where Y1 is dependent on these two. They are more or less linearly related. Data for all these variables are given from 1970 to 2018. We have to forecast values of Y1 for 2040 or 2060 based on these two variables.
What method would you like to suggest (other than a linear regression)?
We have a fact that these series es have a different pattern since 1990. I want to make this 1990-2018 data as prior information and then to find a posterior for Y1. Now, please let me know how to asses this prior distribution?
or any suggestions?
In order to assess the risk of confined space working area, we need to identify all the hazard first. After we identify the hazard, we can determine the risk assessment by risk matrix. In order to reduce the risk matrix, we should determine the risk control by hierarchy control (qualitative). So, the question is, how we could obtain the value of each control quantitatively ? so we can reduce the previous risk assessment.
I am studying 6 behaviours in 2 separate groups of zebras in a zoo, and comparing them. I want to compare how long the 2 groups (as a unit) spend showing each behaviour. I will be observing them for 5 weeks and by the end should have a table like this -
Avg Duration (mins)
Behaviour Gp 1 Gp 2
I'm not interested in within-group comparisons (e.g. Gp 1 forage compared to social), but I am interested in between-group comparisons. However not only would i want to compare foraging between the 2 gps, resting between the 2 gps and so on, but i would also want to compare e.g. gp1 forage with say gp 2 social - between categories of behaviours between the 2 groups.
Now I was thinking ANOVA is partly about whether 1/more variables of various levels have a effect on another variable, but the only variables i have will be time and behaviour. I don't especially think one will have a dircect effect on the other - it's purely just observational. So would I have to do multiple t-tests, since I'd only be looking for differences between 2 means at a time?
I have a question about how i could analyse my data.
My question for my thesis is:
what is the effect of a game on the social skills of people with ass, and is this effect differ in gender, age, work and education level.
In my analysis i did a paired t-test, which allows me to check if the average on the pre-test differs from the average on the post-test.
But how can i do a paired test which analysis:
wheter the effect on the pre-post test differs in gender, work and education level?
Could someone help me, please?
My analysis are done with SPSS
The Delphy study or technique is a survey method designed to structure group opinion and discussion, generate group consensus, and quantify the judgments of experts, asses priorities, or make long-range forecasts (Waltz, Strickland, & Lenz, 2005). If we use this technique to quantify the judgments of experts, how many minimum sample size for this study?
Hi, I am working with Hela cells and my treatment condition is always UV light. One of the assays that I work all the time is ELISA on UV photoproducts, more specifically cyclobutane pyrimidine dimers. The issue is that i cant get to make the assay robust. It seems that it was always unstable in the lab and the way it has been coped with was to basically bombard the bench with a million of experiments and hope that some will work out fine with all the controls behaving as expected and then basically consider your other samples. I am afraid this is not a possibility anymore, I am running out of time and I just need to make sense of this situation as very often than not I just spend weeks on getting my samples and then all this work goes to trash. Anyways, I am pretty confident that the cells are fine, that the irradiation step is correct, and the ELISA per se is fine. I always run 3 technical replicates per condition and they are always satisfactorily similar. I additionally trust my pipetting and the fact that the technical replicates are fine prompts to think that whatever the problem is, it is in the tubes/samples at some point in the process. The hypothesis I am now considering is that maybe UV irradiated DNA is not stable in the sense that, freezing and thawing is able to break the DNA with a articular affinity to the sites of UV photoproducts. this would explain the situation really beautifully, as I can easily see how I am loosing my epitopes in the tube when they break in a somewhat "stochastic" way. It additionally aligns perfectly with the notion that the higher the DNA yield I obtain, these samples behave better, as there is this rule of thumb rule that the higher the concentration of a chemical the more stable it is. I am of course testing this possibility empirically but I would like to know if anyone noticed this or if this is an actual notion in the field. I have tried to find the answers online but cant get any such studies and I am just so baffled that if this is the case how it is possible that no one ever told me before. Additionally, since I am thinking this way could it be that the vortexing is also doing the same thing? this would be a massive pain in the ass, as I handle many samples/experiment and i need to be super precise with the amount of DNA i load, and avoiding vortexing would make the whole thing far more time consuming and additionally could introduce variability due to the loss of precission in my nanodrop quantifications and when I prepare my dilutions from the samples.
Thanks a lot
I am doing free floating immunofluorescent staining on my mouse brain sample.
I've been having difficulty using rodent host (ie. mouse, rat, guinea pig) primary antibodies, and I don't know why. There is no detection under confocal microscope.
Even for the popular antibodies (ie. NeuN from Millipore etc) don't work on my sample.
My samples were previously exposed to 4% PFA (perfusion + overnight post fixation) then washed and stored in 1XPBS with 1% sodium azide.
Tissues were embedded into LMP 3% agar and sliced (50 um), using vibrating microtome.
So far I've tried...(***blocking solution contains 0.1% tritonX-PBS)
- 10% FBS blocking with donkey anti-mouse secondary
- 4% BSA blocking with donkey anti-mouse secondary
- 5% horse serum blocking with donkey anti-mouse secondary
I'd really appreciate if anyone could suggest me protocols/references.
I am currently tryig to do double immunfluorescent staining in myotubes but I could not be able to see a clear negative control.
As you may see in the presentation, I used two different kinds of negative control. 1) no primary antibody 2) Another cell line that should not be expressing desmin (fibroblast). In both of my negative controls I still have strong staining, especially localized in nuclei that I could not get rid of.
%4 PFA is my fixative agent. I am using donkey anti-rabbit (AF488) and donkey anti-goat (AF568) secondary antibodies in 1/5000 dilution.There is not any negative staining in Texas red but in FITC. I do all the washes with %0,5 PBS(T) and 15 minutes. I used 1% FBS and 5% BSA blocking but there was no change. Moreover, I changed the cell type and do the experiment in C2C12 myoblasts but still the same result. Finally, I did desmin staining on its own but negative control was not clear again with donkey anti-rabbit (AF488) but when we do double staining in skeletal muscle tissue, the result was satisfying; no staining observed at negative control.
What could have gone wrong with the cells or antibodies? Any ideas?
Thank you for your kind answers.
Hi I have two dose response curves fitted on aseveral data points made with different drug concentrations. I would like to somehow asses whether the two fitted curves are significantly different.
Could someone propose a method, and if possible a statistics package where it is availalbe?
Thank You in advance
I am looking for some studies or books in (metal or coal) pit lakes that have summarized (if possible) the combined effect of oxygen physical entertainment into bottom thermal zones (eg monimolimnion) and the impact of biogeochemical processes (eg methane, ammonia and hydrogen sulfide oxidation).
Most studies seem to assess these two processes separately or by hydrodynamic modelling. While the latter helps, modelling requires continuous validation. I would be interested in (field) studies integrating both mechanisms to asses the oxygen dynamics (i.e oxygen supply vs oxygen demand).
A great example of what I am looking is:
Thanks – hope to get an answer soon
What do you recommend your patients after rotator cuff repair concerning return to work?
- Do you give general recommendations?
- Do you differ between physical vs. non-physical workload?
-Do you asses the capa to return to work by time and/or functional testing?
I am trying to detect incorporated BrdU in PPFF liver tissue sections of zebra finch. I am using abcam kit for this purpose
I am looking for someone to comment my first stainings, if this staining is only artefact or it is acceptable for collection of data.
My biggest concern is about high signal from end parts of tissues but not in places where tissue were cut off. Is it possible that BrdU is there because of diffusion (BrdU was administrated intraperitoneally 1h before dissection).
I am looking forward any suggestion and comments,
I would like to make an elisa sandwich with this microplate 1) mouse hybridoma supernatants as capture antibodies, 2) a rabbit monoclonal antibody in detection, followed by a donkey secondary antibody
Does anyone have experience with this? Is there a risk of nonspecific binding?
thank you in advance
I have a complete genome sequence of a s.aureus isolated, and is looking for a quick way to asses whether it is identical to one of the 324 s.aureus complete genomes already registred in refseq - if any one have a good way of doing this I would be pleased to know of it.