Entomology - Science topic

Thanks much

Remove legs (1 or 2) prior to rehydration.

Yes.

Have a look at this useful RG link.

Visit also the following useful RG links:

Groups (clusters) can be assessed using the Moran's I index. The Moran Index will help locate spatial correlations between areas and identify typical and atypical habitats

Regards, Sergey

SARS-CoV-2 cannot be transmitted by mosquitoes.

The horn is absolutely not for defense. I’ve tried super hard to get hurt on the horn but the horn does nothing at all. It’s too floppy to do anything defense-related. I’m guessing it has something to do with sensory functions or tricking predators. This would be a cool capstone research project for anyone interested!

I would suggests you can use multivariate analysis, correlation analysis , anova using sas software accordingly you can see the intraction effect.

QuEChERS sample preparation

Hi Murilo Fonseca Ribeiro, try reaching out to Erinn P. Fagan-Jeffries at Uni of South Australia - she works on Microgastrinae. Might be worth a shot.

Best, Andy

It is most likely a species of the family Yponomeutidae, perhaps of the genus Yponomeuta. Knowing the name of the host plant should be easy to ascertain the name of the specie.

Dear Nikola Z. Grujic . See the following useful link:

Sun, X., Zhang, F., Ding, Y. et al. Delimiting species of Protaphorura (Collembola: Onychiuridae): integrative evidence based on morphology, DNA sequences and geography. Sci Rep 7, 8261 (2017). https://doi.org/10.1038/s41598-017-08381-4

Dear Amit Manhas

in the last photo i think - Polyphylla alba

Dear Tyson,

This looks as an interesting topic to be discussed. So, I made a survey to maybe find related papers. You know it is so difficult to find publications that directly dealing with the issue of comparing nocturnal diurnal insects populations. But, I just found two papers one published in 2004 discussing day and night sampling of airborne insect fauna, the other one is published in 2019 discussing the effect of urbanization on diurnal and nocturnal insects abundance and species diversity.

My package pliman might help you with this. Please, see an example of plant disease severity quantification. If the severity of thrips damage has a contrasting coloration, it will be fairly easy to quantify it.

Also check. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6304771/

I need an answer for mine as well:

Locusts in natural ecosystems are an integral component. She plays an important role and ultimately - useful. In agroecosystems, locusts are harmful. The term harmful and useful insect was coined by man to describe the role of each insect in relation to man. But there are no harmful species in nature. The problem of locust harm lies within ourselves. An agroecosystem is a monoculture. When plants are grown in a small area field that are a food source, this causes a surge in the number of the locustes. Not all locusts harm the fields, only 3 species potentially cause crop damage.

Regards, Sergey

Ocimum tenuiflorum (Naai thulasi) plant is used for repelling the activity of mosquitoes. It is highly used in the village side.

Ocimum basilicum

Siriyanangai

I use indicator silica gel inside small plastic containers that can be sealed (for katydids, abdomen content replaced by cotton and enwrapped in toilet paper). The silica gel (blue when dry, pinkish with accumulated moisture) can easily be dried in a small saucepan on a stove or open fire. I keep using the same silica gel for many years.

Dear Robert,

Nice advices to hear from colleagues, but in my opinion you may contact with specialist of your requested group of arthropods from natural history museums around Europe.

You can read these papers

Yes, is Tropinota squalida

Journals of Entomology

1.International Journal of Entomology Research

2.Journal of Asia-Pacific Entomology

3.Journal of Insect Behavior

4.Annual Review of Entomology

5.Agricultural Entomology and Pest Management.

6.Ecological entomology

7.Journal of Agricultural Entomology

Dear Assaf Nir

This effect is also observed in other insects.

I can assume that this is related:

1. When the insect is frightened: by chemical irritants or in case of danger to life.

2. It is possible that an individual examines a new stop using antennas.

Regards, Sergey

Is there any publication so far?

@Patrick Gorring

Thanks for your answer and making me realize that my question is not clear. Should have seen this from David's answer already. My concern is the contamination of the extracted DNA with the pigments, not with DNA from unclean dust. In the past, we had trouble with eye pigments inhibiting PCR, for instance.

I work with entomopathogens fungus in biological control with avocado, berries and citrus pest. But I'm in Mexico, Universidad de Guadalajara.

In the USA, the Californian Universities has a lot of tradition about thouse topics (Berkley, Riverside, Davis, etc)

Agree with Houda Kawas

How can you get it from other country?

Publishing descriptions of new taxa as preprints is definitely a bad idea.

(i) the name would very probably not be made available through use in a preprint

(ii) others could start using the name before the description has been officially published, causing confusion with regard to priority and date of publication

(iii) as Thierry Bouyer pointed out, someone else could come in and quickly describe the same taxon using their own name. Taxonomic vandalism is a thing...

Under the rules of the Code, nothing could modify that (although herpetology has challenged those rules in recent years).

I am searching for Tetragonula iridipennis for the last four years. Still I have not come across this species.

Does this species really occur in India is a debatable question.

Shengbin Chen and the article by Celi et al. 2004 seems to be on RG:

Hi,

In the paper attached, we used DC-Infra, 620.5–645 nm LED. It's not infrared per se, but it's red light, close to the IR spectrum. It was enough to keep our carabid beetles undisturbed by the light source.

In another project on aphids, I have used Sima 36-LED (Hauppauge, NY, USA) and Vision Technologies IR-130 (Houston, TX, USA) infrared lights, coupled with a full spectrum Sony A6000 camera. It worked nicely and we recorded nice footage of insect behavior at night.

Hope this helps,

Kevin

Dear Juan Campos

A mixture of acrylic resins in xylene may be suggested. Drying time 20 - 30 minutes. Does not form bubbles. Ideal optical properties, the refractive index is 1.5. Stable when exposed to moisture, temperature, UV rays.

These are such mounting media as: Elyashev's environment, Kolbe's environment, Hyrax, Pleurax, Styrax, Caedax, Naphrax, ZRAX, Styraplus, Dammaplus

For preparations with Euparal, Malinol, water or oil immersion is not terrible, there is no crystallization.

Regards, Sergey

I confirm it is a soil mite of the Macrochelidae family

Dear Kariyanna B. you're welcome. Please also see this potentially useful article entitled

This article is freely available as public full text on RG.

Dear Kariyanna,

I believe that several other replies here have added useful information for attempting to define this. Though I had some entomology courses and instruct a bit of this in my zoology teachings, I work largely with mammals. Extinction can be difficult, if not impossible, to verify for many species, so a consensus, such as the IUCN description is a useful working application.

In graduate school, a fellow researcher worked on a possible population census of wolverines in the state of Oregon. Though it was assumed that wolverines exist in Oregon, none were definitively sighted for several years. The research was based upon trace evidence - burrows, hair, tracks, and alleged sightings or predation of this species.

I myself assisted a colleague on trace evidence and reports of the thylacine (Thylacinus cynocephalus) in Tasmania. Largely considered to be extinct, but with continued reports, some from reputable sources. Is it extinct? Most mammalogists would say yes, but I am still uncertain with some of the reports and a large amount of suitable habitat that is difficult to access. On the other hand, species such as the Great auk, the passenger pigeon, and Steller's sea cow have no doubt that they are extinct and no sightings have been reported for a century or more.

Here is one reference for insect sampling and potential declines: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069279/

Regards,

Jeb Bevers

The most recient is the Book "Insect systematics and principles of cladoendesis", wich is published in Russian in 2020. English version is in preparing. Information about the adopted basic classification is here: http://www.insecta.bio.spbu.ru/z/sys-ins.htm

Interesting research, although not in my field of work. I have heard about Wolbachia infections in other Diptera possibly causing melanistic non-fertile females. Is this the main reason for the slowing of the spread of Dengue? Is there any research on how Wolbachia will affect other insects?

Best wishes,

Jeroen

Dear Jacob Simons

I know only these sources of literature

Regards, Sergey

Dear Aleksandra Wójcik,

do you still need Ctenolepisma longicaudata alive?

You can send me an email to CDressen@gmx.de for more details.

Best regards,

Christian

Sergey Viktorovich Pushkin right statements. It really depends on the age of the samples. During my PhD thesis, I was dealing with old alcohol arthropods with an age of 15 to 30 years and it was impossible to extract DNA from them as I was really eager to do that. Instead I used a DNA extraction method to clear these specimens to inspect them under microscope for accurate identification for further biodiversity calculations. Just check the link below:

Maybe it is interesting for you colleagues in soil zoology.

Dear Dr Lamia,

the size of cages usually based on what you want to do and how many replicates you will use and if you plan to work on a population setting or just a lab colony for experimental work.

I think we need more details about the experiments to tell you the right size and setup.

good luck

Wesam

Dear Erin Krichilsky I'm just wondering why you are looking for "a journal that accepts publications in two languages or at least is bilingual friendly". What is it good for to publish in different languages? We used to publish our research papers in German back in the 1970's and 1980's, but then we realized that the papers were not read by many researchers abroad. Then we switched to English to make sure that our papers are read worldwide (and eventually cited).

Yang Liangfeng (Naning Agricultural University) and John Morse (Clemson University) published a book with keys on the aquatic macroinvertebrates of China for water quality. This publication already mentioned is in both Chinese and English editions. Author: John C.More & Yang Lianfang & Tian Lixin Language: English ISBN/ISSN: 7563002405 Published on: 1994-01 Paperback

Dear Kara,

The usual concentration for ethanol is 70%. The more percentage makes specimens fragile in long-term even in short-term. About propylene glycol, I should say it may not go to the bottom of vials and it helps to reduce the evaporation of ethanol.

Best, Elaheh

Hello,

Family is Nitidulidae, genus Stelidota, species probably Stelidota multiguttata.

Please check the following articles:

Regards,

Athanasios

Dear Pawan Kumar

SenseFly's drones lineup includes a UAV, whose equipment is aimed at

use in applications related to agriculture. The main feature of the drone

is the presence of a digital camera Canon S110 NIR with a resolution of 12MPix, capable shoot in the range extended towards the near infrared (NIR).

The most famous and common method for assessing the state of the

cover applied to the data, collected using aerial photography, is the calculation of the so-called index NDVI, or Vegetation Index.

NDVI (Normalized Difference Vegetation Index) - relative quantity photosynthetically active biomass, calculated by the formula:

NDVI= NIR-RED

_______

NIR+RED

where NIR is the reflection in the near infrared zone;

RED - reflection in the red region of the spectrum

This formula is based on the fact that high photosynthetic activity, as a rule,

associated with dense vegetation, leads to a decrease in the reflectivity of the object in the red region of the spectrum and to an increase in the near-IR region. Thanks to this, the ability to carry out mapping of vegetation cover based on aerial surveys, ofdentify areas covered and not covered with vegetation, assess density, germination, the state of plants, and with the help of regular monitoring to observe the development of processes in dynamics.

According to the above formula, the vegetation the index can take values ​​from -1 to +1, the value for vegetation it cannot be below zero. Knowing spectral characteristics of certain objects (snow, ice, water, artificial materials) and characteristic for them, the index values ​​can be recognized and classify them on multispectral images.

Data collection with drone eBee AG company senseFly is no different from the previous one eBee models. As a result of aerial photography, the user receives a set of multispectral images in the format TIFFs that are processed by standard means software package for post-processing of UAV data PostFight Terra 3D.

Regards, Sergey Viktorovich Pushkin

Hello,

Below you can find a paper regarding to the study of insect fauna associated with tea gardens in India:

You may get help from the authors (e.g. Bulganin Mitra: https://www.researchgate.net/profile/Bulganin_Mitra/projects) of this publication and you may also refer to the references of this article.

Good luck, Elaheh

Elaheh Daghighi Dear professor, thank you very much for your answer. I wasn't aware of their work. I am going to read their researches and try to communicate with the professors.

I recommend the Egyptian Journal of Biological Pest Control.

Everyone hopes that his project https://www.zin.ru/Animalia/Coleoptera/

following

Javier García Reynaud, I first try my best to store it in multiple locations and drives so as to prevent any data loss. if it happens to occur, though, I use some recovery apps like "recuva" for instance.

Muito obrigado Ronald!

Irei entrar em contato, muito obrigado pela ajuda!

abraços!

Yes, this year 2020, we will be restarting this work (if you read a previously posted answer to this from me, the system added some nonsense that I couldn’t delete/edit) on commercially grown Cucurbita peso (pepo not autoincorrect peso). If others are interested in being part of a team from anywhere in the world, we can share ideas and collaborations.

Hi Nelson. At last, I am almost 100% certain of the species of these nymphs. They belong to Dictyophorus griseus, the grey foam grasshopper. Once you upload a photo of an adult, we can confirm this without doubt.

Can you provide me with a Callosobruchus maculatus lab strain? TY

Here is a useful article for your query.

Management of Climatic Factors for Successful Silkworm (Bombyx mori L.) Crop and Higher Silk Production: A Review

Link: https://www.hindawi.com/journals/psyche/2012/121234/

No. If a number of insect species were really rare in the wild in terms of low number of individuals, they would have become extinct alone - as they couldn't find easily an opposite sex individual to mate and reproduce - and wouldn't wait for a collector-entomologist to do this. ''Rare'' in insects actually is ''hard to find in the wild'', cause of lack of knowledge on their biology or behavior, of difficult to approach/dangerous habitat, etc. All insect species at the right habitat form big to huge populations, from tens of thousands of individuals in a locality for the rarest species, to millions per locality/population for the commonest. Below I will repeat my answer to a similar question here, a few months ago:

''Collections are absolutely necessary and valuable for science.

As proved by so many studies, habitat destruction, climatic change, the tons of pesticides and the general environmental pollution, are the real threats to biodiversity.

As far as I know, in Brazil insect collecting even for scientific purposes is almost totally prohibited (permits very hardly issued), but Bolsonaro's government is burning the Amazon -along with billions of insects- to convert the rainforest into agricultural land.

And it's a total nonsense to compare elephant populations with insect populations.

Ornithoptera alexandrae is threatened with extinction in PNG, cause a great part of its habitat has been converted into palm oil plantations, but CITES ''protects'' it from collectors, doing nothing to protect its habitat, that would be the only way to save it.

Hypocrisy mixed with ignorance..''

Please go through the following RG links.

Carlos; Here is another citation that might be helpful. Jim Des Lauriers

Dear James, thanks again for sharing your experiences. Yes, I think I will create them myself using stuff you can readily buy. Cheers, Philippe

It is important to evaluate the activity of a pure compound. You could use RP-HPLC fractions would be monitored by mass spectrometry. Electrospray ionization mass spectrometry (ESI-MS) is useful to directly determine the exact mass of the antimicrobial peptide in RP-HPLC fractions. Characteristic charge state patterns of the protein or peptide compound upon ESI-MS analyses will reveal further structural information about the the compound. Because ESI-MS is highly sensitive towards the presence of salt, analyses of ion exchange-HPLC fractions requires elimination of inorganic salts, either by the use of volatile buffers or reverse phase cartridges for each separate fraction. In the case of cation exchange-HPLC, salts are required for protein elution. Because several antimicrobial peptides are salt sensitive, there will be a principal risk of getting false-negative results. In some cases, it is possible to overcome this problem by using unconventional, pragmatic conditions for cation exchange-HPLC using volatile buffers such as ammonia salts at an acidic pH. For details see:

I think the aggressors will win. Remember Lord of the Flies?

Not sure. I don't work with pathogens, so I don't know what the difference to an ordinary extraction kit is.

Thanks for these answers. Unfortunately, I can't find it in the animalbase. What I would like to find is something like this paper :

The Handbuch is treated, but only for fascicles dealing with Coleoptera. What I seek is the day, month and year of publication of the fascicles dealing with Orthoptera, to establish priority between two names, published the same year but not by the same author, so I can decide which one has priority (or whether I need to reverse priority, as authorized by IUCZN)

regards,

I agree with Andrew Paul McKenzie Pegman

black is the best netting trap

Hello Faisal; If you tell the community what region of the world your specimens are from they will be able to help. Best regards, Jim Des Lauriers

Thank you Rawa

Hello Trisha; The project you propose is VAST! And to make it more complicated there are many different methods to collect insects that are pretty selective in which groups any particular method targets. Here is a little list of examples that might work in the habitat you are interested in.

1. Pan trap. A low pan with water about 1 cm deep. A drop of dish soap will increase the effectiveness. Pans of different colors attract different groups.

2. As Mr. Duarte suggests, fish is attractive to flies and some beetles.

3. Peanut butter, bits of canned meat, or cookie crumbs smeared on the bark of trees will attract many ants.

4. Fermenting sugar is attractive to a large array of insects.

5. Feces in a little cup placed in the bottom of a larger cup attracts several groups of beetles. Not all feces are equally attractive! It depends on what the collector has eaten recently!

6. At night a bright light sitting on a white sheet will be amazingly attractive.

The list goes on. There are various books on collecting methods. Here is one example. https://www.cabdirect.org/cabdirect/abstract/19941104344

Happy collecting! I envy the diversity you will encounter, Jim Des Lauriers