Endophytic Fungi - Science topic
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Questions related to Endophytic Fungi
I am looking for methods to distinguish between these two cases. For instance, in literature, the Botryosphaeriaceae fungi have an endophytic stage, on the other hand, some Colletotrichum species have a quiescent infection.
I am working on micropropagation techniques in wood tree plants, but while doing the endophytic fungi shows dominance, can anyone suggest how to inhibit the endophytic fungi?
I isolated 5 endophytic fungi and started with fermentation of 21 days with Potato dextrose broth media. I completed with extraction with ethyl acetate as per literature review. Now I wanted to identify the chemical constituents present in 5 extract which i got from endophytic fungi, so which methodology is applicable?
We are now working on the chemical content of the total extract of endophytic fungi. While analyzing the results of the GC-MS assay, it is found that there is Carbendazim in the extract of fungi. Could fungi synthesize Carbendazim?
What is the difference between the large scale culture and small scale culture in endophytic fungi culture ?
Isolation of endophytic fungi carried out for medicinal plant and started with fermentation for further extraction of secondary metabolites, is design expert is applicable for above mentioned fermentation processe?
I am a PhD student working on the caracterization of secondary metabolites of endophytic fungi. I would like to ask you about the lyophilization conditions of extract of endophytic fungus specially temperature and pressure ?!!
Many thanks for your help.
We are trying to perform BSLA on our endophytic fungi extracts so we have 1.6 mg/mL of our extracts. Should our positive ctrl have the same initial conc as our extracts?
What are the growth hormonal parameters that can be estimated from endophytic fungi except for auxin (IAA)?? Please also mention their colorimetric protocol for estimation or quantification.
Habit: Endophytes on Tulsi
Location: South India
We often face the problem of fungal Contamination of perrenial crops, tree crops. We used different pricedures for pretreatments and surface sterilization. However, the endophyte fungi is still a Major problem for Tissue Culture of tree crops. Could you please share your positive experience how to overcome heavy endophyte infection to establish reliable micropropagation protocol for tree crops?
I have students working on an endophytic fungi project. One student out of curiosity found a Lion's-mane Mushroom (Hericium erinaceus), surface sterilized it and placed it on PDA. I am not familiar with fungi living within other fungi (or the correct terminology). I have attached an image of the PDA plate.
For the molecular identification of fungus,
why usually use the three regions internal transcribed spacers (ITS), Small subunit (SSU), and large subunit (LSU)?
Endophytic fungi are ubiquitous to plants and are mainly members of Ascomycota or their mitosporic fungi, and some taxa of Basidiomycota, Zygomycota, and Oomycota. These endophytic fungi reside within living tissues of the host and establish symbiotic relationships . The colonization of plants by these fungi varies considerably; and some medicinal plants are reported to harbor more endophytes . The endophytes associated with medicinal plants are known to acquire the potential to produce the active principles for which the host is known .They are a rich source of functional secondary metabolites that include flavonoids, terpenoids, steroids, phenol, phenyl propanoids, quinines, indole derivatives, amines, alkaloids, amides, pyrrolizidines, sesquiterpenes, diterpenes, lignans, isocoumarinated chloride derivatives, metabolites, phenolic derivatives , aliphatic compounds.
im currently in the phase of extracting metabolites, specifically for antibacterial screening from endophytic fungi. i tried ethyl acetate, but after 1 week it's still not dry.
Can anybody explain me the interaction of endophytic fungi and the host in the production of secondary metabolites. I just need to know that if the endophytic fungi and the host plant producing same metabolites means what type of interaction or mechanism is going on between them and if not means what...!
Under semi-hydroponic conditions certain genotypes repeatedly showed specific fungal growth, while others not. Is it because of variability at endophytic fungi level?
I'm working on endophytic fungi, I want to do a co-culture of three endophytic fungi that were isolated from the flax plant how to inoculate germinated seeds flax by endophytic fungi? If anyone can help that would great! Thank you
Fungal spores on surface of explants can be eradicated by surface sterilization with a fungicide. But, how to control endophytic fungal contamination form explants during plant tissue culture. Please suggest any chemical which can be added in tissue culture media or any method to control endophytic fungi from explants.
During metabarcoding of endophytic fungi, many seem to use ITS primer pairs that are not (fully) selective for fungi. We experienced that such primers also amplify the ITS region of an in vitro (non-colonized) clone of the host. In field samples, the plant ITS is the most pronounced. Does this prevent successful metabarcoding on Illumina MiSeq (or similar)? Is a highly specific primer truly necessary? Any hints?
Actually, we have isolated a endophytic fungi from medicinal plants. We started to identify them by using a macroscopic method. However, it is not enough and we recently acquired a microscope (normal and inverse). So that, we need to learn how to use the two microscopes. And we need a protocol
Looking forward to reading your answers.
Thanks a lot
What do you think about switching from endophyte fungi to parasite?
Is that a behaviour or other else?
What are the factors of this switching?
How could be studied?
I am using PCR to test leaf and root samples of forbs and grasses for endophytic colonization by Hygrocybe fungi (cf. Tello et al. 2014). I sterilized my samples according to the method of Unterseher and Schnittler (2009): 2 minutes in 70% ethanol, 5 minutes in 1% NaOCl, 1 minute in 70% ethanol again, and 3 minutes rinsing in distilled water.
I am finding an unexpectedly high rate of positive results in leaves compared to roots. I am concerned that this could be due to contamination from spores: I collected the samples at the same time Hygrocybe fungi were fruiting, so presumably there would have been a lot of spores in the vicinity. Was my sterilization protocol adequate to get rid of such spores?
Thanks very much in advance!
Dear all, Good day, currently I am practicing extracting total DNA using different fungi. My main purpose is to extract total DNA and send the extracted DNA for 16S Amplicon sequencing (ITS-1 and ITS-4)
In our lab, we often get contamination from endophytic fungi and bacteria in sugarcane explants (young leaf sheath and buds). I will like to know about experience of other related researchers in this type of issue.
I want to culture Fusarium oxysporum. Can anyone provide me detailed protocol for Fusarium oxysporum culture?
I really need to carry out endophytic fungi isolation from some medicinal plants in Nigeria. Where can i successfully carry it out in Nigeria. What are the reasons for rinsing the plant parts intended for fungi isolation with
1. Distilled water
2. Ethanol and
3. Sodium hypochlorite
I am working on grass fungal endophytes bioprospecting, I have isolated the sec. metabolites by using Ethyl acetate and Methanol for culture filtrate and mycelial mat but I am having a small doubt that why can't we isolate fungal metabolites by sequential isolation by using various solvents. If yes, then which are the solvents used for it.
I have used ethyl acetate to macerate mycelial mat of one of my endophytic isolate from sea weed. I want to run column. suggest me how to pack (solvent for dissolving silica gel) and run the same.
what are the different types of secondary metabolites produced by endophytic fungi ?
I need to identify some isolates of endophytic fungi through the spores, but I don't know which literature is best suited for better identification.
What is the most used literature?
It concludes that they are of the genus Pestalotiopsis and Colletotrichum, although it wanted identification at the species level
I have to mix mycelia of an endophytic fungi with the seeds of barley and incubate it in an optimum temperature until germination.
Question 1: Is there any method of mixing mycelia and barley seeds together until germination of barley seeds?
Question 2: The germination rate if very low, Is there any international manual for seed testing?
I am studying whether or not endophytic fungi can serve as a sustainable source of secondary metabolites useful in the treatment of the neurological issues associated with addiction, whether by antagonism at opioid receptor sites, inhibition of metabolic hormones, or some other means. I was wondering what assays might be useful for assessing the biological activity of any compounds I am able to isolate. One plan is to use a purified secondary metabolite as a hapten in a hapten-antigen complex and to use an ELISA kit as a model for human opioid receptors. However, since I've only ever studied physics before, I'm fairly new to all this, so any advice would be much appreciated.
I plated endophytic fungi and bacteria on agar. At 3 time periods, I traced the outline of the growth area on the outside of the plate. I used ImageJ to calculate the area for each measurement. Is there a way to calculate growth rate based on all of these area measurements? What formula would I use? Or should I simply use the starting and final measurement?
Update: I ended up square root transforming my area measurements and then finding the slope of these three area measurements. I am using this slope value in my ANOVA comparisons. This method is from: Estrada, C., Rojas, E., Wcislo, W., and S. A. Van Bael. 2014. Fungal endophyte effects on leaf chemistry alter the in vitro growth rates of leaf-cutting ants’ fungal mutualist, Leucocoprinus gongylophorus. Fungal Ecology 8, 37-45.
In Plant Tissue Culture, after surface sterilization of explants with Bavistin, Tween80, Ethanol, Mercuric cholride or watever....only the surface of the explants are sterile...once cuts are made in the explants, endophytic fungi comes out and contaminates the explant..Can I surface sterilize the explant after causing wound/cut to the explant to achieve sterility or would this prevent callus formation?
I understand there's disagreement regarding the similarity threshold for fungal OTUs, be it 95%, 97%, or 90-99%. In the literature, anyone of these threshold may be cast as adequate or too conservative.
For my thesis, I want to determine the fungal communities among tree-samples with next-gen sequencing.
From your own experiences, which similarity threshold(s) were most appropriate?
Edit: To clarify, I am using the ion torrent platform Ion S5 for sequences 200-400 bp long. Compared the Ion PGMʻs 5 million sequencing capacity, the S5 530-chipʻs maxes out at 20 million sequences.
I am trying to analysis the data of Preliminary antibacterial screening of endophytic fungi (inhibition zone in mm) but i have no idea how to use it.
I'm working on transmission of endophytic fungi in grasses. I need to observe the fungal hyphae growth in grass tissues. Hence, I need an best protocol for the study.
I am isolating fungal endophytes from grass species, i got some fungal endophytes which i am failed to identify them because they are coming only on moist blotter but not on other nutritive medias hence I'm struggling to make pure culture and also in molecular identification.
I have synthesized nanoparticles from biomass of an endyphtic fungi and I want test it for anticancer
I am working on endophytic fungi and i want to test minimum inhibitory concentration for my endophytic fungal extracts so any body explain the simpler procedure for me.
Apart from isolation of endophytic fungi many a times we need to detect fungal endophytes directly into the plant tissue. I would like to ask my peers to shed some light on this query.
Can anyone suggest me the media for the fast growth of endophytic fungi ,other than potato dextose ,becoz my isolates taking too time to grow in PDB?
I am working on Bio prospecting of endophytic fungi in perennial grass species. for my further work i have isolated the endophytic fungi from grasses and i need an simple protocol for DNA isolation of fungal endophytes.
I'm looking for review articles which are focused on the phylogenetic analysis of South african (even madagascar island) Euphorbiaceae in relation to endophytic fungi hosted in theses phytotaxa
I am working on endosymbiotic bacteria residing in endophytic fungi. My query is about the number of bacterial species. Does one fungal strain harbor only one species of bacteria?
Hello there! is anybody working on endophytic fungi? I am currently conducting my research about endophytic fungi from mangrove and i am facing a problem in identification of fungus under microscope. it is very hard to look for a field guide on how to identify the fungi.
Karrikinolide is a bicyclic, heteroatomic organic compound found in smoke. Smoke contains aromatic hydrocarbons, chlorine compounds and aldehydes that may be responsible in reducing the infestation of endophytic fungi to a certain extent, as these smoke compounds show similar chemical structures to those of known fungicidal compounds.
Hello there, we managed to transform endophyte (fungi) with reporter gene (gfp). The endophyte of interest lives in the outer space of plant cell. When we tried to investigate the colonization of our endophyte in planta using confocal microscope, the signal for gfp was so weak and the autoflourecent signal was quite strong. Does anyone know a good technique for fixing and clearing which dose not denature affect the signal of gfp?
I want to study the effect of endophyte on uptake cadmium in barley.
I am working on endophytes in cotton. I need to isolate endophytic fungi and bacteria from leaves, stems and roots. Plz suggest a protocol where we can easily exclude saprophytes and isolate only the endophytes.
During the process of my project on biological control I found one fungal species belong to Basidiomycetes has high antagonistic ability against many plant pathogenic fungi and also toward a particular types of perennial weeds. I would have appreciated very much if can present any details about the fungi that have more antagonistic activity rather than Trichoderma and at the same time has the ability as potential bio-herbicides. Thank you
a healthy sweet potato root(tuber) takes how many days to be completely rotted by any fungus or multiple fungi.
I want to identify ochratoxingenic strains——Aspergillus carbonarius. Does it enough for Aspergillus carbonarius just use the species-specific CARBO1/CARBO2 primers? Do I also need to do the ITS or the beta-tubulin simultaneously?
In a study done by Russell et al. (2011), Pestalotiopsis microspora, an endophytic fungi was discovered to have polyurethane (PU) plastic degradation capabilities due to its ability to produce the enzyme polyurethanase, which is a serine hydrolase. According to Global Industry Analysts, 13,650.00 kilotons of various PU products in 2010 were produced and over two million tons of PU is produced in the EU alone every year, showing how big the PU production and PU pollution are. The ability of P. microspora to produce polyurethanase shows that it has potential for bioremediation of PU.
What are specific possible ways to use P. microspora and/or its polyurethanase enzyme as a large-scale bioremediator to contribute significantly in decreasing polyurethane pollution in the environment?
Russell JR, Huang J, Anand P, Kucera K, Sandoval AG, Dantzler KW, Hickman DS, Jee J, Kimovec FM, Koppstein D, et al. 2011. Biodegradation of polyester polyurethane by endophytic fungi. Applied and Environmental Microbiology. [Internet]. [cited 20 Apr 2017]; 82(20): 6080-6090. Available from: http://aem.asm.org/content/77/17/6076.full
ISOPA. 2017. Polyurethanes More Facts and Figures. ISOPA. [Internet]. [cited 20 Apr 2017]. Available from: http://www.polyurethanes.org/en/what-is-it/fact-figures/more-facts-and-figures
I want to detect fungal toxins gene or toxigenic fungi directly without using the cultural method. Can I or Can't?
I want to purify my compounds , I want to know the protocol/procedure , if anyone has kindly refer to me.
I'm working of endophytes. I'm doing my extraction procedure with a little bit confusion. is that necessary that we have to do the primary extraction with ethyl acetate.
In most articles they suggested to do in ethyl acetate.
as i dont know what will be compound present in the microbe, can u tell me what should i do?
Isolating DNA from obligate fungi like powdery mildew from leaf surfaces can be tricky as the scrapping of fungal mat /spores from the leaf surface may contaminated with saprophytes?
molecular identification of Aspergillus falvus.
NS1 ,C18 primers is universal primer for identification of Aspergillus species?
Yield of mass culture is good. But after extraction of fungal mass with ethyl alcohol or other solvent like methanol, yield is very low.
I Want to identify some fungal species by basic (Conventional) combination methods, please suggest.
Hi....I am working on ipomoviruses infecting vegetables in Iraq and I wonder if there is any primer set specific for detection of the genus Ipomovirus? As the only available option for the genus detection is Potyvirididae specific primer set.
I need to identify bees of Pennsylvania to the Genus level, I'm wondering if anyone knows of any good guides. I ran across a website called Discoverlife.org but I'm not sure how accurate it is or if it can be trusted. Any help is appreciated.
please tell me selective medium for isolating fusarium udum?
I want to prepared fungal extracts from my cultured endophytic fungi, which the extracts will be used to test for its antioxidant activity (H2O2 scavenging effect and reducing power assay). Is there any way to prepare the extract via filtration? And, also, when testing for the antioxidant activity, do I use the filtrate or the mycelia from the broth culture?
My field of research is in Postharvest Pathology of fruits, we work with the fungi and the fruit and then with the interaction pathogen-fruit. Our goal is to propose alternative systems of fungicides to control postharvest fungi in tropical fruits. I work in Nayarit, Mexico and is a tropical a subtropical conditions and the presence of fungi is a big problem.
Best Regards from Nayarit, Mexico.
Dr. Porfirio Gutierrez-Martinez
List of Toxic secondary metabolites secreted during the pathogenic infection of Fusarium species.
Where can i find the method on how to separate the foliicolous lichen from the leaf tissue and where can i find the identification key for foliicolous lichen?
I would like to know which are criteria to say that a fungi is an endophyte
while working on rice fungal endophytes i found these pathogens, but it is very difficult to differentiate them based on their cultural and morphological characteristics
Where are you going to grow them to see their growth analysis? are you going to see them in controlled condition or in open natural environment which is unfamiliar to the plants?
why we isolate fungus bacteria and spend money for their molecular identification? as they are already on internet .
I am currently work on endophytic fungi, using rice solid static cultures. One of my fungus shown blue methanol extract. Blue color appear after the molded media immerse using methanol over night and disappear after third day at room temperature. Any idea to isolate this compound? I think it is polar and unstable compound.
I am trying to identify some wild fungal species using ITS and D1-D2 regions, but in the hits from NCBI getting even different genera in both regions (ITS&D1/D2) for the same species.
Any helpful suggestion ?
I have some pure mangrove endophytic fungal colonies. I want to test them against test pathogenic strains by modified disk diffusion method by cutting agar block from the fungal colony instead of antibiotic impregnated disk. For that I have grown the test strains into nutrient broth culture and want to use them directly by cotton swab. But now I am confused weather inoculums of test strains as 0.5 McFarland standard with saline water will need to be prepared according to rational Kirby Bauer test or the test strains can be directly used from the broth culture. I have tested the growth of the test strains directly from the broth culture without matching with McFarland standard and they gave dense uniform layer on nutrient agar plate. Does it essential to prepare inoculums matching with 0.5 McFarland standard in such case and if I won’t maintain the McFarland standard what will be the possible problems because making inoculums of test strains as 0.5 McFarland for each period is time consuming and greater risk of contamination in my lab facility.