• Lackson Kasonka added an answer:
    Does cardiac pulsation is the only way to determine fetal survival at 8.4 weeks of pregnancy?

    A 33 yr woman with 8.4 weeks estimated gestational age with last menstrual period revealed following reports on transvaginal ultra-sonography:

    1. Bulky uterus

    2. Single gestation sac of about 5.43X4.62X3.34 cm size in uterine cavity.

    3. Crown to rump length of embryo is 16.3 mm which correspond to 8.2 weeks of gestation.

    4. Normal yolk sac seen in gestation sac.

    5. Trophoblastic rim is normal in thickness and ecogenicity. A small anechoic area seen right laterally.

    6. Gestation sac contains single embroyo without cardiac pulsations.

    Pathology impression: finding suggstive of missed abortion. Minimal separation of membranes noted.

    Physician recommend to undergo D&C (dilation and curettage). What would be the actual action to be taken under such circumstances i.e.  D&C or wait for few weeks. Is such kind of sonography reports are always true.

    Lackson Kasonka

    Though fetal viability has been determined by US scan as early as 5 weeks in most centers, this diagnosis can delay up to 9-10 weeks in some due to challenges associated with the model of the equipment and the expertise of the operator. A repeat scan within a few days by another expert may reaffirm the diagnosis of missed abortion.

  • Majid Tarokh added an answer:
    What is the best housekeeping genes in endometrial tissue?

    in endometriosis

    Majid Tarokh
    Thanks my friend
  • Sangappa Chadchan added an answer:
    Which cell type are the cells in the lumen of an endometriosis lesion?
    Which cell type are the cells in the lumen of an endometriosis lesion?
    Sangappa Chadchan

    Endometrial epithelial cells and stromal are most common cell types in endometriosis

  • Volker Baumann added an answer:
    Any inputs in doing time course experiments to check for miRNA effects post transfection in macrophages on regulation of pro-inflammatory cytokines?

    miRNAs used:  mimics for both 125b and let7B (will work with inhibitors also since this is a product of an endometriosis paper done previously)

    transfection reagent: RNAimax

    Volker Baumann

    If you want to detect the alterations in the cytokine abundance I would suggest to perform an Elisa or Elisplot with your treated macrophages. This would be the best way to see if your treatment has a functional consequence on them.

  • Astrit Gashi added an answer:
    Embryotoxicity of the rice peptide-RGD-any data in humans?

    have read about bioactive peptides in food with potential health benefits and much research in this area but also have seen references to a RICE peptide=RGD--that has embryotoxicity at least in lab animals. This RGD is described as a LAMININ-like peptide involved in immune mediated reproductive failure. I am also aware of reports of anti-LAMININ antibodies found in women with endometriosis and infertility. Is there any evidence of this RGD embryotoxicity in humans, or any cross reactivity beween the RICE RGD and HUMAN LAMININ---I am totally unaware of any dietary implications related to RICE on pregnancy---any data at all in this context?

    Astrit Gashi

    The value of physiologically and biologically active motifs present in proteins
    is considered critically taking into attention many aspects including the mechanisms
    of action of the peptide in a body, the processes of protein hydrolysis and the inhibition of them during enzymatic digestion, the absorption of peptides into a bloodstream, the length and a structure of the peptide. Although the enormous growth on studies concerning the structure and the activity of the motives had been observed, some mechanisms of bioactivities of peptides are still not fully discovered. However, the information concerning the function and structure of bioactive proteins and peptides gathered in the various database resources interconnected with the mathematical descriptions of the biological phenomena is a powerful tool to assign the protein to a group of more potent biomacromolecules. BIOPEP is one of the tools which contributes to such approach.
    The strategy of analysis of proteins as the precursors of bioactive peptides involving
    BIOPEP procedure enables to classify proteins to be a rich/poor source of motifs with biological and physiological functions..This approach can be successfully
    applied to introduce nutraceuticals as the valuable source of peptides with biological

  • Massimo Giuliani added an answer:
    Has anyone recommedations about psychological aspects of endometriosis?

    I am especially searching for psychological and/or cultural causes of endometriosis. Thank you for your answers!

    Massimo Giuliani

    No studies are available on associations between endometriosis and psychological and cultural factors. What we know is that women with endometriosis suffer from functional psychological disorders similar to those of women with infertility due to other causes . They have body image disorders , self-esteem problems , sexual disorders and disorders of attention and concentration . Neuropsychological disorders who accuse women with endrometriosis can be placed in direct association with the inflammatory processes associated with the disease , as described in other patients with infectious diseases .

  • Christopher R Harlow added an answer:
    Which kind of cell lines are more suitable to perform a study in endometriosis?

    I need two cell lines to perform a study in endometriosis and normal endometrium but which kind of cell lines are more suitable to perform this kind of study? 

    thank you in advance!!

    Christopher R Harlow

    Met5a mesothelial cell line might be worth trying to look at interaction between endometrial and mesothelial cells.

  • Annemarie Schweizer-Arau added an answer:
    Which is the best primary treatment for infertile patients with endometriosis III or IV? IVF?
    Infertile patients and Endometriosis III or IV seen for the first time.
    Annemarie Schweizer-Arau

    Natural Ivf is a low cost effective primary treatment for endometriosis for infertile patients

  • Márcia Mendonça Carneiro added an answer:
    I am interested in an endometriosis-associated subfertility model. Can you provide the model?

    Besides abdominal pain, subfertility is the second key symptom in patients suffering from endometriosis. Details of the model I am searchng for a described in the attached flyer.

    Márcia Mendonça Carneiro

    I am very interested in such a study. Currently I coordintate the Endometriosis team at the Federal University of Minas Gerais in Brazil. In addiiton to the public service I also work with a muldiscplinary team at the private sector. We have some research on the area but the questions of subfertility in Endometriosis remain ellusive. Patients are heterogeneous and many times other causes of infertility (eg Male factor) are found.I will be more than happy to contribute to your research.

  • Anthony G Gordon added an answer:
    What are the most pressing research/clinical questions in endometriosis?

    We at the Endometriosis Association are beginning another capital campaign to fund research as we've done throughout our 35 year history (over $29 million direct & indirect so far).  We're pondering how best to prioritize research aims and are interested in feedback from the research community.

    Anthony G Gordon

    Answer: Finding out how endometrial tissue spreads, as the blood vascular and lymphatic vessels do not explain its pattern of spread.   This must be by an unrecognised anatomical pathway from the bottom to the top of the body.  The same route is involved by vicarious menstruation, and many other substances which end up in unusual places, eg urine and bile in the ear canal.   The vital clue to this pathway is the intense pain that originates when substances pass up it.   In the first instance, little money would be needed, just a thorough review of all the literature, old and new.  

  • Rajshree Dayanand Katke added an answer:
    Can you think of any valid shared risk factors for colorectal cancer and endometriosis?

    Either positively or negatively associated factors.

    Rajshree Dayanand Katke

    yes the probable positively association.Estrogen , Hereditory factors contribute the etiology.

  • H. Ram added an answer:
    What are main basic causes and mechanisms of endometriosis of estrogen dependent female reproductive organs?
    Endometrium is estrogen dependent reproductive tissue.
    H. Ram

    Thank you Dr. Safoura, but there is any prescribed therapy for this.

  • Grant Gallagher added an answer:
    Does anyone know of available human or mouse endometrial cell lines (not endometrial adenocarcinomas)?
    Grant Gallagher


  • Manjeet Kaur Sharma added an answer:
    Immortalized human endometrial epithelial and stromal cells from normal and/or endometriosis cases.
    Can anyone share an aliquot of immortalized endometrial stromal and epithelial cells (not from cancers like the ISHIKAWA cells)? I also need cells from ectopic endometrium. I am willing to sign the MTA and the necessary formalities, it's quite urgent.
    Manjeet Kaur Sharma
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  • Denea Heimstead asked a question:
    Does anyone know of or is anyone doing any research on the long-term effects of Lupron after a woman has ended its treatment?
    Thanks in advance.
  • Osman Zarroug added an answer:
    Subculturing mouse endometrial cells?
    I am struggling with subculturing mouse endometrial cells using trypsin/EDTA and a cell scraper. I have no problem isolating the cells, but when they become confluent and they are due to be subcultured, they stop growing and die following trypsinisation/scraping.
    Can anyone suggest any alternative method I could use to successfully lift off the cells and seed them into a new flask?
    Osman Zarroug
    Well Matthew I guess we have cracked it!!!!
    The new medium we have tried (SensiCell DEMEM/F12) worked quite well. We have managed so far to do 2 passages.
  • Rakesh Arora added an answer:
    What biosimilars are helpful in management of endometriosis?
    Endometriosis needs to be addressed with proper therapeutic molecules. Biosimilars are probably the answer. What phytochemicals are promising in this effort?
    Rakesh Arora
    LHRH Analogue called NAferelin acetate has widely been accepted USFDA aaproved drug other than any biosimilar. It is modified nine-mer synthetic peptide. I am not aware of any phytochemical for the same.
  • Rakesh Yashroy added an answer:
    In inflammatory biomarker research, shouldn't circadian oscillations and stress be taken into cinsideration?
    Levels of cytokines/chemokines can vary depending on the time of day the blood sample is collected, stress level of the patient and how the blood was collected. Accounting for these factors is important in my opinion. Also, why aren't Il-10 and IL-12 results also reported as a ratio to give a better indication of the immune status in endometriosis?
    Rakesh Yashroy
    Yes! Most inflammatory traumas trouble during night hours. May also relate to BMR during activity and sleep. Promising proposal.
  • Aniko Huizer-Pajkos or Pajkos added an answer:
    What is the better treatment for a patient who has endometriosis and Lupus?
    Dear colleagues,
    I need help because I have recieved a 39 years old patient who had been submitted to Abdominal Hysterectomy for endometriosis in January, 2013 but she maintains abdominal-pelvic pain chart and to make worse all that, she has Lupus so I have doubts concerning what might be the best approach for the case and if is posible to use progesterone for her. I think it is not a surgical case because there is no identified focus at MR.
    Thanks for your help
    Aniko Huizer-Pajkos or Pajkos
    I am a scientist with rare autoimmune condition not that dissimilar to lupus. I manage my own condition under the guidance of my doctors as not much is know about my condition. I also went through difficult endometriosis surgery which dramatically improved pain but did not eliminate it. Based on my knowledge and experience, I agree with JAir and vasculitis would certainly be one thing to consider. It will not be welcomed from a doctor but based on my experience learning to live with the pain makes it more manageable.
  • Matthew D Rosser added an answer:
    What are the genes related to etiology of endometriosis?
    Matthew D Rosser
    Hi Priyanka, endometriosis is a largely multi-factoral conditions involving the disregulation of many genes and molecular pathways, however here are some papers that I think will definately be of use to you http://www.ncbi.nlm.nih.gov/pubmed/21193803 http://www.ncbi.nlm.nih.gov/pubmed/20104428 http://www.ncbi.nlm.nih.gov/pubmed/16816328 http://www.ncbi.nlm.nih.gov/pubmed/23006437 http://www.ncbi.nlm.nih.gov/pubmed/22556377 http://www.ncbi.nlm.nih.gov/pubmed/22203846

    Best Regards
  • Rajshree Dayanand Katke added an answer:
    Endometrial tissue in parametrial blood vessels?
    Recently I've experienced strange finding in hysterectomy specimen with multiple myomas and adenomyosis and endometriosis in the serosal surface. Several clusters of endometrial tissue was found in the parametrial blood vessels and attached to the vessel lumen which doesn't seem to be just tissue contamination. What do I have to consider in this lesion?
    Rajshree Dayanand Katke

     I think this is the Dissemination of Endometriosis on the serosal surface,i have come across such condition. Bleeding and the Adhesions is the surgical diffculties in these cases.even in the fibroids also we may get Leiomyomatous Dissemination in some  cases though rare.

  • Angela Szpojda asked a question:
    Does lycopene reduce endometriosis risk in Mediterrean countries?
    There is research that shows that lycopene can be supportive in reducing endometriosis. If that is the case do people from Mediterranean countries have a lower risk as they are likely to have more Lycopene in their diets?

About Endometriosis

A condition in which functional endometrial tissue is present outside the UTERUS. It is often confined to the PELVIS involving the OVARY, the ligaments, cul-de-sac, and the uterovesical peritoneum.

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