Endometriosis - Science topic

The pelvic peritoneum consists of two layers: the parietal layer, which lines the pelvic cavity walls, and the visceral layer, which covers the pelvic organs. The mesothelial layer, composed mainly of mesothelial cells, is the epithelial layer of the peritoneum. Mesothelial cells produce a serous fluid that lubricates the peritoneal surfaces, reducing friction between organs during movement within the pelvic cavity. This lubrication is crucial for the smooth functioning of organs such as the intestines, bladder, and reproductive organs.Additionally, the mesothelial layer provides protection against infection, injury, and friction-induced damage to pelvic organs, helping to maintain the integrity of the pelvic cavity and prevent the spread of infections or inflammation.Mesothelial cells also play a role in absorption and secretion within the peritoneal cavity, helping to maintain fluid balance by absorbing excess fluid, ions, and small molecules. They can secrete factors involved in inflammation, immune responses, and tissue repair.

Omran S Habib . In this sit it will be a cohort study!

Thankyou for your reply. Can you pls explain what is this factor 0.5

Also, kindly check:

Sorry, this topic is not in my field of science

Dear doctor Anahita,

i am in a good agreement with doctor Kumar answer's.. by using Gnrh analogues hormone.

You can also contact the authors of the following paper:

They have performed a lot of work, including STR authentication on this particular cell line. The corresponding author is highly responsive.

1. Trends in Endometriosis among Laparoscopic Patients in Multiple Hospitals in Northern India: A 3-Year Review

2. Study of endometriosis in women of reproductive age, laparoscopic

management and its outcome

HI Abdoulie, I managed to find below article on Linkedin as posted by world leader Endometriosis and Pelvic Pain Awareness: Infertility, Suicidal Ideation, and Cancer - Journal of Obstetrics and Gynaecology Canada (jogc.com) ,

A survey would be interesting indeed!!! Thanks Lisa

It would be better to compare benign and malignant conditions in the same organ/tissue: endometriosis vs endometrial cancer, for example, instead of uterus compared with ovary tissue.

According to the latest ESHRE guideline on the management of endometriosis, a Cochrane review on the preoperative hormonal treatment concluded that there was no evidence of a benefit of preoperative medical therapy on the outcome of surgery, but it also acknowledges that in clinical practice, surgeons prescribe preoperative medical treatment with GnRH analogues as this can facilitate surgery due to reduced inflammation, vascularisation of endometriosis lesions and adhesions. However, there are no controlled studies supporting this.

By the way, medical treatment should be offered preoperatively to women with painful symptoms in the waiting period before surgery, with the purpose of reducing pain before, not after, surgery.

Radiotherapy is generally not used in treatment of endometriosis. Treatment of endometriosis is by medicines or surgery depending on patients symptoms and extent of endometriosis. radiotherapy is mainly used to treat malignant conditions.

Hey Matthew,

Very neat topic!!

In regards to alternative/complementary at-home interventions, two came to mind:

1) There has been growing evidence suggesting that moderate cannabis use could help mitigate pain in endo through several MOA. Several cross-sec studies have shown that cannabis is one of the most frequently used non-pharmaceutical interventions amongst patients with endo. Cannabinoid receptors (CB1) have been found at the somatic fibres adjacent to the lesion site. Several murine studies have shown the potential of cannabinoids in drastically reducing pain scores and even reducing the lesion.

In general, especially in areas such as Canada or America, cannabis is widely accessible. Although the caveat remains, as there is not enough literature to fully support the clause due to variation in cannabis and route of administration.

2) I've noticed that the most frequently suggested intervention by women with endo over social media is changing diet. This could be a great way to reduce inflammation at home and improve overall health in some cases.

Yes, we can.

Endometriosis can cause infertility through different mechanisms : 1. Pelvic, tubal and uterine adhesions. 2. endometrial tissues secreted toxic substances which negatively affect spermatoza and decrease their fertilizing ability. 3. Vaginisimus, vaginal nodules or mass. 4. Ovulatory dysfunction in 10% of cases.

There is no any concrete scientific evidence to prove that lycopene reduces the risk of endometriosis in Mediterrean countries.

The ovariectomised rodent model is used to determine the role of ovarian hormones (estrogens and progesterone) in whatever thing is being studied. In most cases, it is preferable to ovariectomise all groups and have one group (or more as needed) with added estrogen at a fixed dose, as opposed to have intact animals (i.e. not ovariectomised) in the same experiment. This is because the ovary goes through cycles of more or less hormone release. For most experiments is more useful to know exactly the amount of estrogens the animal is exposed to.

For the study about the surgical success in endometriosis , you must consider too the NCT and the grade

This is an interesting review that addresses your question [28553145]. In brief, depression may be more prominent in women with severe symptomatology and also women with concurrent depression experience precipitated pain.

It is important to differentiate the type of cyst. Each type has different etiology. Generally ovarian cysts can be classified into these categories: follicular cyst, corpus luteum cyst; endometrioma, dermoid cyct and cystadenoma. Polycystic ovarian (PCO) condition has many follicles that do not rupture.

Follicular cysts are due to disturbed LH release. Instead of rupturing the follicle, it lutenizes the inner follicular wall and the follicle stays on the ovary causing further hormonal disturbances like increased estrogen secretion and irregularity in periods.

Endometriomas are dark chocolate colour fluid filled cysts that develop on the ovary. They are linked to the endometriosis, etiology of which is of considerable debate. Menstrual contents spill out of the fallopian tubes and grow wherever they attach around the ovaries or uterus.

Corpus luteum cyst develops after follicle has ruptured. This type of cyst usually does not cause much hormonal disturbances and disappear after corpus luteum lysis. Dermoid cysts may contain fat or other tissue inside. Cystadenoma is non-cancerous growth on the outside of ovary.

Because you are doing research on the ovarian cysts, I suggest conducting thorough literature search as much work has already been done especially on endometriosis and polycystic ovarian syndrome (PCOS).

Serum free generally means no serum. 

Many people include 0.1% BSA or FBS into their starvation protocol. Not because of stability but as a carrier protein since without it low concentrations of growth factors (either autocrine or added) will get absorbed by the plastic tubes and dishes. 

Some cells need a bit of FBS to survive, but many cancer cell lines can be in serum free media forever. 

Agree with@Huda Omran

Endometriosis can spread via blood and lymphatic vessels. This underestimated fact can explain several rare symptoms or histopathological findings, e.g. endometriosis within the sciatic nerve or endometriosis at very distant localisations. Please, see also: Tempfer CB et al. Lymphatic spread of endometriosis to pelvic sentinel lymph nodes: a prospective clinical study. Fertil Steril. 2011 Sep;96(3):692-6. doi: 10.1016/j.fertnstert.2011.06.070 or Zamurovic M. Rare extrapelvic endometriosis on iliac vein wall--diagnosis and treatment. Clin Exp Obstet Gynecol. 2014;41(3):349-50.

Endometriosis and endometritis are sometimes confused due to the word similarity but they are totally different entities.  Endometriosis has not been shown to be associated with an infection.  It is associated with inflammation.  

That is why there no antimicrobial drug for endometriosis.  

Good luck with your studies.

stroma is responsible for the activity of the disease , while glands isn`t active. over expression of VEGF is mandatory for neovascularisation and proliferation of the disease. but the epithelium and the glands also, express VEGF as well.

refer to : doi: 10.1093/humrep/13.6.1686 

Thanks my friend

While waiting and re-scanning is probably safe, it is also unnecessary. Given the advanced size of the foetus, the lack of heartbeat is inconsistent with normal pregnancy and is diagnostic of nonviable pregnancy (missed abortion)

Diagnostic criteria for nonviable pregnancy early in the first trimester.  N Engl J Med. 2013 Oct 10;369(15):1443-51.

Endometrial epithelial cells and stromal are most common cell types in endometriosis

If you want to detect the alterations in the cytokine abundance I would suggest to perform an Elisa or Elisplot with your treated macrophages. This would be the best way to see if your treatment has a functional consequence on them.

The value of physiologically and biologically active motifs present in proteins

is considered critically taking into attention many aspects including the mechanisms

of action of the peptide in a body, the processes of protein hydrolysis and the inhibition of them during enzymatic digestion, the absorption of peptides into a bloodstream, the length and a structure of the peptide. Although the enormous growth on studies concerning the structure and the activity of the motives had been observed, some mechanisms of bioactivities of peptides are still not fully discovered. However, the information concerning the function and structure of bioactive proteins and peptides gathered in the various database resources interconnected with the mathematical descriptions of the biological phenomena is a powerful tool to assign the protein to a group of more potent biomacromolecules. BIOPEP is one of the tools which contributes to such approach.

The strategy of analysis of proteins as the precursors of bioactive peptides involving

BIOPEP procedure enables to classify proteins to be a rich/poor source of motifs with biological and physiological functions..This approach can be successfully

applied to introduce nutraceuticals as the valuable source of peptides with biological

activities.

I don't think there are such causes. However, cultural and psychological causes influence the quality of life related to health and the perception of health in women with endometriosis. 

Met5a mesothelial cell line might be worth trying to look at interaction between endometrial and mesothelial cells.

I am very interested in such a study. Currently I coordintate the Endometriosis team at the Federal University of Minas Gerais in Brazil. In addiiton to the public service I also work with a muldiscplinary team at the private sector. We have some research on the area but the questions of subfertility in Endometriosis remain ellusive. Patients are heterogeneous and many times other causes of infertility (eg Male factor) are found.I will be more than happy to contribute to your research.

Natural Ivf is a low cost effective primary treatment for endometriosis for infertile patients

one of crucial issues which necessitate  deep insight in endometriosis is the discrepancy between clinical presentations and available diagnostic methods and so many cases with endometriosis related symptomatology came back with negative laparoscopic findings because  of microscopic lesions, healing lesions, or early stages ( still under control ) lesions.

Thank you Dr. Safoura, but there is any prescribed therapy for this.

thanks!

Well Matthew I guess we have cracked it!!!!

The new medium we have tried (SensiCell DEMEM/F12) worked quite well. We have managed so far to do 2 passages.

LHRH Analogue called NAferelin acetate has widely been accepted USFDA aaproved drug other than any biosimilar. It is modified nine-mer synthetic peptide. I am not aware of any phytochemical for the same.

Yes! Most inflammatory traumas trouble during night hours. May also relate to BMR during activity and sleep. Promising proposal.

I am a scientist with rare autoimmune condition not that dissimilar to lupus. I manage my own condition under the guidance of my doctors as not much is know about my condition. I also went through difficult endometriosis surgery which dramatically improved pain but did not eliminate it. Based on my knowledge and experience, I agree with JAir and vasculitis would certainly be one thing to consider. It will not be welcomed from a doctor but based on my experience learning to live with the pain makes it more manageable.

Hi Priyanka, endometriosis is a largely multi-factoral conditions involving the disregulation of many genes and molecular pathways, however here are some papers that I think will definately be of use to you http://www.ncbi.nlm.nih.gov/pubmed/21193803 http://www.ncbi.nlm.nih.gov/pubmed/20104428 http://www.ncbi.nlm.nih.gov/pubmed/16816328 http://www.ncbi.nlm.nih.gov/pubmed/23006437 http://www.ncbi.nlm.nih.gov/pubmed/22556377 http://www.ncbi.nlm.nih.gov/pubmed/22203846

Best Regards

Matt

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