Embryo Cryopreservation - Science topic

dear friends , Is any authenticated data or research is available that shows approximate or exact number of in vitro produced competent embryos lost every year due to unavailability of recipient or other reason in humans , bovines or other species.

Thank you

A 34-year-old female underwent robotic pancreatoduodenectomy in June 2018. Recovery was uneventful and pathology disclosed poorly differentiated adenocarcinoma of the Ampulla of Vater T3bN1M0. She was trying to become pregnant of her first child when she received this diagnosis. MDT decided for adjuvant chemotherapy, but she decided to preserve her fertility by embryo cryopreservation. She then received 12 cycles of Folfirinox. She is well with no signs of disease 18 months after operation and fully recovered. The main question is when is the appropriate time to conceive? Should we wait 5 years? 2 years? 3 years? I did not find any answer in the current literature with this type of tumor.

I want to use Cryotop technique for embryo vitrification. Did you have any experience about this issue? I know that Kitazato has commercial kits for this aim. Have you used this product? Or can we prepare vitrification solutions or straws for this aim?

Thank you for your answers... 

So we are trying to collect a large amount of mouse embryos to do RIP-seq. I'm wondering whether I can freeze the embryos directly at -80 after each collection or I have to do the slow freeze/ fast thaw method which is normally used to cryopreservation/revive the embryos? Thanks.

Can anyone recommend an appropriate vibration criteria for IVF clinics (including the storage of embryos) in addition to the VC-A/VC-B criteria for optical equipment? Thank you in advance for your help

Cryoprotectants contain electronegative groups capable of forming hydrogen bonds with water molecules. Thus, if the water molecules are strongly linked to cryoprotectant so what are the more used?

Samples are fixed, embedded and frozen following a standard protocol though when sectioned at 10 um the tissue is flimsy, delicate, and missing sections.

A number publications exist on the in vitro development of embryos after IVF of cryopreserved ovine or caprine oocytes.

Has anybody published the birth of offspring after IVF of cryopreserved ovine or caprine oocytes?

Does anyone use this older type of freezer? I am wondering if I can use a "normal" container with LN2 adjusted by steel cap etc. as a source of nitrogen for freezer, or if it is necessary to buy a special container with regulation of pressure inside it?

osmotic injury, sperm recovery, freze-thawing, viability, DNA damage, fertility potential

Hi to all developmental biology experts,

I would highly appreciate your advice regarding the shipping of mouse embryos (age E12), from US to Israel. After transportation embryos should be embedded in OCT, cryosectioned and used for Immuno.

What makes this specific situation problematic is that frozen or refrigirated shippment is not an option! Only option is to ship them on room temperature. After the PFA step, what would be the best medium to transport embryos in - PBS, 15,30% Sucrose or something else?

Paraffin embedding is not an option also.

Oocyte-cumolus comlpex is vital for further maturation of Oocyte nucleus in vitro

I want to examine preimplantation stage mouse embryos with LSCM and I have to fix them.

I understand that the temperature -196C is determined for this process as the chemical reactions stop in this temperature. I am not sure that this is the only temperature at which this process is conducted. What happens if the cell is frozen below and beyond this temperature? Is there a safe margin for this process? Does the cell type affect the process?