Science topic
Eggs - Science topic
Animal reproductive bodies, or the contents thereof, used as food. The concept is differentiated from OVUM, the anatomic or physiologic entity.
Questions related to Eggs
Hello everyone, just spotted this on mulberry fruit. Is this some kind of insect's egg or part of fruit.
I saw in a literature that the author used different research methods to build an aging model. Then the author's research started from fish eggs. I don't quite understand if it is necessary to start from fish eggs and what the purpose of doing so is. What is the difference between adult fish and juvenile fish in the aging model? Could everyone tell me?
We stored Sitotroga cerealella eggs in a refrigerator at 5–6°C, but the eggs did not hatch. I want to know if the low temperature affected their viability. What is the maximum temperature range that allows the eggs to remain viable during storage without impacting their ability to hatch? The temperature difference was 7-10C in storage and rearing facility
Hi everybody. I need to extract RNA from ~50 C. elegans eggs (after bleaching) or L1. For large quantity I usually freeze Eggs/larva in M9 in -80, grind in mortar with pestle in liquid nitrogen, add Trizol and then extract using Zymo RNA-miniprep kit. For small quantity of eggs/L1 grinding in mortar with pestle is not an option. Just cycles of freeze-thaw in Trizol give very low RNA quantity, obviously due to insufficient breakage of eggshells/cuticles. Will be very happy to get a working protocol Suggestions are welcomed too.
i wont to know that the number of days or weeks that the egg of the mosquito for how long can survive in the dry weather and after how many days can be spoiled or frozen specially the dry season.
I am currently working on a project focused on gastrointestinal (GI) parasites in donkeys, Sri Lanka. We have collected stool samples and identified various eggs of GI parasites. However, we would appreciate confirmation from experts to ensure the accuracy of our identifications.
If anyone has experience or expertise in identifying GI parasite eggs in donkeys, your assistance would be immensely valuable. We are looking for confirmation or any additional insights that could help us verify our findings.
Thank you in advance for your help!
Chathuri
Hi! Can you help me regarding this matter, I am going to do an Egg Albumin Denaturation Assay on the formulated ointment with 5g plant extract. The ointment's base is beeswax and olive oil. However, I cannot find a good solvent to dissolved the wax. Please recommend. Thank you
Whether any study has been undertaken to evaluate the effect of consumption of Omega-3 Eggs on the cognition and general health of primary school children?
Sri Lanka almost went bankrupt. Sri Lankan Government that ran on loans turned to IMF for more loans. It is doubtful that more loans are going to solve the financial crisis in Sri Lanka unless the government accountability is restored, and the Cash-Printer is DISABLED for good and adopt US Dollar or Euro in place of the Sri Lankan Rupee. Can the newly elected Government turn the country around and make country self-sufficient?
Today, price of a dozen of eggs in Sri Lanka is more than 700 Rs. Price of a loaf of bread is 300 Rs. If it had not been on-line payment, a backpack full of bills has to be carried for a weekly grocery trip. How did the Sri Lankan Rupee become such a joke so fast?
Regarding Egg membranes as model cell membranes for diffusion experiments.
which is better, the synthetic membranes or egg membrane
Hello,
I am a beginner conducting experiments using Daphnia magna.
I've noticed that when female water fleas release eggs, sometimes they shed their exoskeleton and the eggs are released externally, and other times they hold the eggs inside their bodies until the young are able to swim, at which point the offspring push their way out of the mother's body.
In the case of the former, I heard that when eggs are released externally, the difference in osmotic pressure between the mother's body and the external medium affects the egg release.
It seems that in the case of Daphnia magna, the babies are released in the latter way, where they develop inside the mother's body until they are ready to swim.
In that case, doesn't the external medium enter the mother's body where the young are growing? From the images I've seen, it doesn't seem completely isolated from the outside, so I am curious.
I would appreciate any advice.....
Thank you
Hi. We have been observing this strange cell death morphology during months. Cells die and acquire a "fried egg" morphology as it is shown in picture (A431 cell line). When it happens, all the cells die alike. It appears randomly in cell plates and not in all the experiments, but we are thinking that affected-wells distribution could be repeated, but it does not occur in all the plates. It has nothing to do with cell treatment, because it has been observed also in control cells. If you have some advice, please anwer :)
Thank you!
The black spot was observed after peeling egg.
Compare and Contrast Plant and Food Metaphors in English with Those in Other Languages
Here are some English proverbs based on plants and food:
1. Don’t put all of your eggs in one basket.
2. He jumped out of the frying pan into the fire.
3. He spilled the beans.
4. He’s nutty as a fruit cake, or He wants everything, from soup to nuts.
5. It’s as easy as pie, or She’s upper crust.
6. She’s buttering him up.
7. It’s like comparing apples and oranges.
8. Life is a box of chocolates.
9. That’s how the cookie crumbles.
10. There’s no accounting for taste.
11. You have to crack some eggs to make an omelet.
Check out the attached PowerPoint about Plant and Food Metaphors, and then discuss Plant and Food Metaphors in English and other languages.
Since last few weeks we are facing a strange problem. When we put a large number of C. elegans eggs on a E. coli OP50 containing plate, most of the eggs do not hatch. Worms coming out from the remaining few eggs are not able to move towards the bacterial food in the center of the Petri dish.
We have tried reducing concentration of the bleaching solution used during synchronization. Even additional washing steps, and replacing the bacterial and worm culture with new ones has all been tried, but nothing could fix the problem.
Any troubleshooting suggestions from the C. elegans research community will be highly appreciated.
In order to view/ find the laid thrip eggs is there any methods is available, if yes, suggest me the methods.
generally, zebrafishes will breed and produce a great number of eggs effectively, but in recent time we are facing trouble in zebrafish breeding, we are maintaining the optimal conditions, day and night cycle, frequency of breeding, diet, breeding ratio, water and temperature. still we are facing issue, any one can suggest something to reconsider and any other factors to look into.
If then which medicine production and name of the medicine what's is that?
We have been rearing Rockefeller mosquitoes for many years in our laboratory without any problems, but lately, in spite of good viability of eggs and unchanged environmental conditions (27°C, 70%RH), the mosquitoes spend too much time in larval stages (until 3 weeks. Last year, the average time from L1 to L4 was 1 week).
Is there any physiological or genetic cause that generate this effect on the colony? This fact will have any effect on adult biology?
Thanks in advance
Myriam Salazar
Bogota, Colombia
Effect of heating and nutritional value loss
"How do we understand special relativity?"
The Quantum FFF Model differences: What are the main differences of Q-FFFTheory with the standard model? 1, A Fermion repelling- and producing electric dark matter black hole. 2, An electric dark matter black hole splitting Big Bang with a 12x distant symmetric instant entangled raspberry multiverse result, each with copy Lyman Alpha forests. 3, Fermions are real propeller shaped rigid convertible strings with dual spin and also instant multiverse entanglement ( Charge Parity symmetric) . 4, The vacuum is a dense tetrahedral shaped lattice with dual oscillating massless Higgs particles ( dark energy). 5, All particles have consciousness by their instant entanglement relation between 12 copy universes, however, humans have about 500 m.sec retardation to veto an act. ( Benjamin Libet) It was Abdus Salam who proposed that quarks and leptons should have a sub-quantum level structure, and that they are compound hardrock particles with a specific non-zero sized form. Jean Paul Vigier postulated that quarks and leptons are "pushed around" by an energetic sea of vacuum particles. 6 David Bohm suggested in contrast with The "Copenhagen interpretation", that reality is not created by the eye of the human observer, and second: elementary particles should be "guided by a pilot wave". John Bell argued that the motion of mass related to the surrounding vacuum reference frame, should originate real "Lorentz-transformations", and also real relativistic measurable contraction. Richard Feynman postulated the idea of an all pervading energetic quantum vacuum. He rejected it, because it should originate resistance for every mass in motion, relative to the reference frame of the quantum vacuum. However, I postulate the strange and counter intuitive possibility, that this resistance for mass in motion, can be compensated, if we combine the ideas of Vigier, Bell, Bohm and Salam, and a new dual universal Bohmian "pilot wave", which is interpreted as the EPR correlation (or Big Bang entanglement) between individual elementary anti-mirror particles, living in dual universes.
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Wolfgang Konle added a reply
April 17
Fred-Rick Schermer "He (Einstein) used the term Spacetime to help declare aspects about the gravitational effects of matter, in specifics the anomalies as for instance seen with the perihelion of Mercury."
Spacetime is a term in relativity theory which only indicates that the structure of space and time is related.
"Once a person accepts that matter came about in the Big Bang model, then one cannot declare at the same time that unification is real as well."
The big bang model is bullshit.
The only relevant model is about an eternal universe. Instead of a big bang only cyclic bangs happen, which affect about 10% of the mass of the universe. The restricted cyclic bangs release astronomic signs, which we misinterprete as traces of a big bang.
The cyclic bangs resolve all black holes, once every 20 billion years, and retransform their matter to new star fuel.
All arguments against that model of the eternal cyclic universe can be disproven in a factual way.
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Sergey Shevchenko added a reply
April 17
The thread question rather in detail is scientifically answered in SS 5 posts series on page 1.
Dear Fred-Rick
- in that
“…Matter’s spacetime is fundamentally absolute."
I fully agree here but only if I understand you correctly. It is matter that is the source for spacetime; space and time are actually not part of the discussion. Rather, all words apply to the behavior of matter and nothing of these words applies to space or time. though the words are implying they are...”
- you understand what is in the SS posts above correctly, however only in certain sense, though.
To understand more it is necessary to read at least first few pages in https://www.researchgate.net/publication/354418793_The_Informational_Conception_and_the_Base_of_Physics, where it is explained what are absolutely fundamental phenomena/notions “Space” and “Time”; and their concrete actualizations in every concrete informational pattern/system - concrete spaces and time
[ while in the Shevchenko-Tokarevsky’s philosophical 2007 “The Information as Absolute” conception, recent version of the basic paper see
- it is rigorously proven that there exist nothing else than some informational patterns/systems of the patterns that are elements of the absolutely fundamental and absolutely infinite “Information” Set; including Matter is nothing else that some the Set’s element.
At that the utmost general definition of the absolutely fundamental phenomenon/notion “Information” is
“Information is something that is constructed in accordance with the set/system of absolutely fundamental Rules, Possibilities, Quantities, etc. – the set/system “Logos” in the conception” .]
I.e. the “Logos” set elements “make something to be an information”, and any/every concrete pattern/system, including Matter, is made absolutely obligatorily by some concrete “composition of the “Logos” elements actualizations”.
“Space” and “Time” are just the “Logos” set [besides any informational pattern/system “is made” also, first of all, by “Logos” elements “Energy”, “Change”, “Logical Rules”] and their actualizations in concrete cases are concrete space, that can have any number of concrete “space dimensions” [the number is equal to number of degreases of freedom at change of state of a pattern/system], and the unique in the Set “time dimension”.
Any concrete pattern/system can exist and change, in a system its elements interact, etc., only in its concrete space and time.
So, including any “behavior of matter” is possible only in some space, Matter is rather simple logical system that is based on binary reversible logics, and so Matter’s utmost universal space has 4 dimensions – X,Y,Z are necessary – “allow” to make binary operations, cτ- dimension allows reverse binary operation.
Correspodingly the space and time intervals between elements, motion of elements in space and time dimensions, etc., are absolutely necessary for existence of everything in Matter –
- and at description and analysis of what exists and happens in Matter. If you don’t know these data, you by no means can describe and analyze “behavior of matter”.
Besides, really it is fundamentally senseless to ask “what appeared earlier – Matter or its spacetime”, Matter could appeared only in its spacetime, which – as logical possibility to create, and to exist of, Matter – existed as a part of the Set’s spacetime, which contains all spaces of all Its elements – and one time dimension;
- while the Set exists absolutely “eternally”, having no Beginning and no End, since absolutely fundamentally - logically - cannot be non-existent.
Cheers
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Fred-Rick Schermer added a reply
April 18
Wolfgang Konle
Thank you, Wolfgang, I understand better now where you are coming from.
The model you embrace is not the model I embrace, and this helps us understand what each of us is saying.
I do not support the cyclic universe; it was a one-time event in which the prior state broke at a fundamental level. Hence my saying that, once we have an omelet, we know that the egg was broken. There is no return to the original state available.
An eternal universe requires that we have evidence for that eternal aspect. We do not have that evidence. I will not stand in non-scientific grounds. I will only stand with my feet on the ground (even when that is on a planet floating through space) and I will not stand with my feet on space.
It is illogical to have matter be eternal. There is no indication that matter is eternal, rather we have a clear understanding that matter did begin with hydrogen (and helium), and how all other elements arrived in various subsequent fashions.
Matter returning to an immaterial state is not supported by scientific evidence. At best, it can be read in models, but then we need to discuss the value of these models. I am not convinced that black holes are actually real, but that is a different discussion.
I am standing with the scientific evidence, Wolfgang. I do not extrapolate it into additional dimensions. I may not be the best scientist, but I will not stand outside the scientific realm.
Thank you again for explaining where you are standing.
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Cosmin Visan added a reply
20 hours ago
Spacetime doesn't exist. "Spacetime" is just an idea in consciousness.
… Read more
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Wolfgang Konle added a reply
15 hours ago
Fred-Rick Schermer "It is illogical to have matter be eternal. There is no indication that matter is eternal, rather we have a clear understanding that matter did begin with hydrogen (and helium), and how all other elements arrived in various subsequent fashions."
We do not have the faintest valid explanation about a possible creation of electrons and protons without the simultaneous creation of positrons and antiprotons.
This fact and the unlimited lifetime of electrons and most atomic nuclei leaves us with the only possible conclusion that matter must be and must have been eternal.
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Fred-Rick Schermer added a reply
13 hours ago
Wolfgang Konle
Thank you for that answer, Wolfgang. We are not thinking fully along the same lines. That is very clarifying; after all, communication is difficult enough.
Let me find out if this is about language or if we really say something else.
I can say that the unlimited lifetime of electrons points to a potentially eternal nature of Energy. This is what you may want to say, but you placed eternal with Matter, which is in my model not possible.
If the transformation of Energy into Matter occurred some 13.8 billion years ago, then the term eternal cannot be applied to Matter. A (fundamental) transformation undermines being able to use the word eternal.
It is not known how old Energy is, so I cannot make the claim that Energy is, or is not eternal. We just don't know. Yet Matter is a result. We know therefore that it cannot be eternal; it was produced at one point in time, something new got produced from something old(er).
--
In my model, I do not need to start with the same amount of matter as antimatter because the starting point for matter begins under extreme circumstances. It is not an ordinary circumstance. Antimatter will occur, but it is a circumstantial byproduct.
We can discuss this further if you want, but the special circumstance is more interesting now.
There was a special circumstance in which the prior normal conditions of whatever or however energy existed before caused itself to move toward that special circumstance. This could have been done in a happenstance manner, or in a directed manner. But it was a step that led to a fundamental undermining, with either option we pick.
As a consequence, (some) original energy got deformed during these special conditions, and a quark soup got created (to keep the storyline simple). Then, the special conditions were reversed, everything back to normal, yet the deformed quarks were not able to return to their original state. They were and are deformed packages of original energy.
The reversal of the special conditions occurred at the CMBR, when conditions were normal again. Here the quarks aligned themselves immediately into neutrons and protons.
That is the point Zeus overthrew Cronus, if you allow me to throw in some Greek mythology as support that I am not thinking up something structurally never considered before. Where immaterial Energy was first the only circumstance for energy, with the transformation of some energy into quarks, they actually took the lead.
Matter became the center of energy (quarks in nuclei of atoms). Everything else remaining in the original energized state then falling in place, aligning themselves with the quarks in the center.
Yes, electrons then also part of the original immaterial energy, but then pulled into the deformed reality of the energized quarks, causing the tip of that iceberg to become material.
Preprint On Quarks Explaining Our Universe
Cosmin Visan added a reply:::
Wolfgang Konle Matter doesn't exist. "Matter" is just an idea in consciousness.
Cosmin Visan added a reply:::
Fred-Rick Schermer You really do have a communication problem. Have you tried a psychotherapist ?
The eggs of cuckoos have thicker shells than their hosts'. Unfortunately, I haven't found published data on the egg shell thickness of the cuckoo finch (Anomalospiza imberbis). If someone has such an information (published or not), would be really thankful if to be shared.
It's not a question, but if anyone has a photo of the eggs of the fish called Harpagifer bispinis, please share it with me.
I am trying to detect Avian leukosis virus from egg albumin by ELISA (Cat#CK422B, BioChek, the UK). I am getting OD of positive control - OD of negative control below 0.50, which should be more than 0.50 for test should be valid. What may be the causes? Thanks in advance.
More specifically: We are working on breeding some mycophagous Erotylidae and I would be very interested in obtaining eggs for life cycle studies. Thanks
I need to separate egg membrane and calcium form a egg, Pleas help me.
I want to graft the cancer cells i.e, MCF7 on cam of egg. To do this I need to have some scaffold. In a paper i read about matrigel, is there any other alternative of matrigel or can we graft the cells directly onto the cam without any scaffold?
A interesting question: i want to create a egg model in Abaqus including 3 parts: eggshell(shell element), egg white(solid element) and egg yolk (solid element).
They are wrap each other. However, what contact and constraint i should use in ?
My idea is: using embedment constraint between white and yolk, however, i dont know what used between the shell and white?
Any idea will be very appreciate, thank you!
Hello,
When I heat egg albumin or yolk with concentrated Hydrochloric acid I get a violet fine precipitate ..... why does it appear?
My conclusion is heaven is universal. Respectfully, how does the uniqueness of each sperm relate to the probability reincarnation? The fact that each sperm fertilizing the egg results in a different individual being conceived, and then maybe born, causes reincarnation to fall in probability. The recycling of consciousness(the theme of reincarnation) is further lowered in probability by the uniqueness of each sperm. Also each sperm, forming a completely different individual than another sperm, creates a completely different consciousness than another sperm would have. How could someone who had died have their consciousness recycled through the genitals of others? Thus, the uniqueness of each sperm( and the randomness of which sperm fertilizes which egg) causes reincarnation to be a less parsimonious explanation of the afterlife than heaven without reincarnation is. Sources:
Denaturation assessments on egg albumin and bovine serum albumin suggest a link between tissue protein denaturation in living organisms and the onset of inflammatory complications. In this assay, a 5 ml reaction mixture containing egg albumin, phosphate buffer saline, and the test sample is heated, incubated, and cooled. Absorbance at 660 nm is recorded for both the reaction mixture and the standard (diclofenac sodium), with a phosphate buffer solution serving as the control.
Each time I conduct this assay, the absorbance readings consistently provide negative values. What could be the cause of this phenomenon? Why is the absorbance of the egg/bovine serum albumin denaturation assay taken at 660 nm?
Hello, I'm working on my MSc project related to Parasitology of Marine mammals, particularly cetaceans. I've been identifying some eggs and adult specimens by morphological methodologies, however, there are some of them that I can't identify, for that reason I was wondering if some of you could help me to identify some of these parasite eggs.
To start, it is important to refer that the samples were taken from stranded cetaceans who were already dead and to which cetacean species I found each egg.
I've attached a PDF Document with 13 Figures, each figure has specified to which species it belongs. I hope you can help me with this challenge.
I wish you all a Merry Christmas and a Happy New Year.
Dear parasitologyst researchers, In order to research the helminth eggs in treated water. We looking the most species found and the method used in the laboratory to identify them.
Thanks
Determination of vitamin d in egg yolks. The eggs are collected from farms with different husbandry conditions. Caged, free range and organic eggs. I have data collected today.
Concentration of Vitamin D2 in whole egg yolk (ug) MEAN for Caged/Free range/ Organic
Whole egg yolk (g) MEAN for Caged/Free range/ Organic
- Types of Eggs Total vitamin D2 in yolk (ug) Whole egg yolk mean per egg (g)
- Happy Egg 0.057 17.0
- Caged Egg 0.087 16.4
- Purely Organic 0.104 17.1
We managed a necropsy on a wild land tortoise that arrived at our faculity hospital due to a traffic accident and was near death. I found small nematodes clustered in its intestines, as depicted in the photograph. I am sharing microscopic images with you as well. The adults seemed to belong to the Oxyuridae family, but I found it challenging to closely resemble their eggs. Does anyone recognize the species or at least genus?
+4
Dear Sir,
It appears that some females possess alae like structures in the Toxocara genus, while others do not. Initially, I thought they might be different species, but it seems they may be the same species. Due to the similarities in the organs they inhabit within their hosts, and other morphological features, I am almost certain they belong to the same species. Do you have any insights at the genus level at least?
Best regards
I want to starting up a Pieris brassicae culture. I collected almost 10 egg packages from the cabbage fields different times. All larvaes are became butterflies but all butterflies were female. There were spots on its wings. Butterflies didn't lay eggs. I think I can not start culture because I can not find male butterfly. Am I just unlucky or is there some other reason? Thanks in advance.
I will use the Oxford Nanopore MinIon.
I will just access the COI gene region.
- What is the largest order of insects in terms of species diversity? Answer: b) Coleoptera
- In insects, what is the purpose of the tracheal system? Answer: a) Oxygen transport
- Which insect order includes butterflies and moths? Answer: d) Lepidoptera
- What is the term for the process of shedding an old exoskeleton to allow for growth in insects? Answer: c) Ecdysis
- Which insect is known for producing light through a chemical reaction in its abdomen? Answer: b) Firefly
- What is the primary function of an insect's antennae? Answer: d) Sensory perception
- Which of the following is NOT a stage in complete metamorphosis? Answer: a) Nymph
- What is the primary role of the queen in a social insect colony, such as bees or ants? Answer: c) Egg laying
- Which insect order includes grasshoppers, crickets, and katydids? Answer: a) Orthoptera
- What is the term for the external skeleton of an insect? Answer: b) Exoskeleton
- Which of the following is NOT a type of bee in a colony? Answer: d) Larva
- What is the name of the process in which an insect transforms from a pupa into an adult? Answer: c) Metamorphosis
- What is the primary purpose of the proboscis in butterflies and moths? Answer: a) Feeding
- Which insect order includes mosquitoes and flies? Answer: b) Diptera
- What is the term for the collective behavior of a group of locusts migrating in search of food? Answer: d) Swarming
- What is the specialized structure used by some insects for producing sound? Answer: b) Stridulatory organ
- In entomology, what does the term "entomopathogenic" refer to? Answer: d) Insects that cause diseases in other insects
- Which insect order includes bees, wasps, and ants? Answer: c) Hymenoptera
- What is the primary function of the ovipositor in female insects? Answer: a) Egg laying
- Which order of insects is characterized by having two pairs of wings and includes beetles? Answer: b) Coleoptera
- What is the term for the specialized structure on a spider's abdomen used for silk production? Answer: c) Spinneret
- Which insect order includes dragonflies and damselflies? Answer: d) Odonata
- What is the primary function of the forewings in most insects? Answer: d) Flight
- Which of the following is a major product produced by honey bees? Answer: c) Honey
- What is the purpose of the cerci, which are paired appendages found on the abdomen of some insects? Answer: b) Sensory perception
- What is the primary function of the pheromones produced by insects? Answer: a) Communication
- Which insect order includes termites, known for their ability to digest cellulose? Answer: b) Isoptera
- What is the term for the process of an insect transforming from an egg to a nymph without a pupal stage? Answer: b) Incomplete metamorphosis
- Which insect order includes true bugs and cicadas? Answer: a) Hemiptera
- What is the purpose of the spiracles in insects? Answer: d) Gaseous exchange
I will be analyzing the vitamin D content of chicken eggs. I have been advised to use Perkin Elmer Gas Chromatography as the most appropriate gas chromatograph for this experiment. What steps are involved in the preparation of my samples? Could anyone assist me with the equipment list as well?
Whatever the synchonizing hatching method used, do you have some results about hatching rate curve (dynamic) for every hour about Aedes aegypti or Aedes albopictus?
thanks
I need to isolate RNA from fish eggs and fish tissues.
This is my first time dealing with fish samples.
Can anyone help me out.
I need to increase egg laying in BSF brooding cage and increase their mating frequency.
I am trying to use recycled materials to grow microgrren seeds. so I will use egg cartons and Sawdust. But I am worry about microorganisms on these material.
Hi
Is this gelatinous mass familiar to anyone? Which genus/ species does it belong to? It was found in Norway in shallow waters close to shore, and has the consistency of nemerteans/ molluscs. It is about 2 cm in diameter. When cut in half, most of it is just gelatinous mass. However, roughly in the centre is 20-30 tiny eggs. Could it be the egg mass of e.g. nemerteans/ nematodes or molluscs?
Looking forward to your response.
Kind regards, Halldis R.
I did CAM assay recently. 0th day the vessels where intact and good. The drug incubation was of 48hours. After incubation, every eggs appeared different. Couldn't able to distinguish which is contaminated, which is actually the result. Have attached the images of some of the eggs after incubation
Please I need your response on this.
We are working on salmonid fish eggs and would like to isolate RNA from them. The samples of eggs are stored on -80C, but the individual sample is too big (too many eggs in tube for a single isolation) and needs to be aliquoted somehow. Does anyone have a suggestion how to aliquote and store samples to avoid negative effects of freeze thaw cycles on eggs and RNA? We would like to keep the rest of the eggs for potential additonal RNA isolation.
Thank you!
Simona
Hi. We have fish eggs that have been fixed in 95% ETOH at room temperature for 1-2 years. Can these still be used for genetic barcoding? Thank you.
The temperature of keeping eggs is 27 degrees Celsius and the humidity of the environment is 70%.
when eggs of Fasciola hepatica are found in the faces of person with non symptoms of fasciolasis ,in such case the diagnosis how can be confirmed ?
Recently, I found some parasitic eggs in a patient's urine sample and the patient is taking immunosuppressive drugs for SLE.
When a microscopic examination of the urine found the parasitic eggs and I think it is schistosome eggs and not Entrobiasis infections but the epidemiological conditions, it is impossible to Schistosome eggs.
I kept the sample in 70% ethanol.
How to confirm further investigation and what are the possible parasitic infection?
how those insects manage to get inside the fruit during the ripening stage .
Egg is considered to be one of the healthy food. Is use of egg is harmful for patients with ischemic heart disease.
I am currently evaluating the possibility of studying Culex pipiens in an ecotoxicological context by applying the dynamic energy budget (DEB) theory and effect modeling. For this, I am searching available life-history data of Culex pipiens to parameterize a standard DEB model. Specifically, I need length (or weight) data and egg numbers over time at different temperatures.
Does someone work with this species or know where to find growth and reproduction data measured over time?
I propagated the IBDV virus in the SPF eggs via the CAM route. The amount of harvested virus is limited so I want to ask if is there anyone with experience in doing realtime PCR instead of doing actual titration in eggs.
These were viewed using a light microscope with a 40x magnification.
For adult immersion test what is average weight of engorged females of Rhipicephalus microplus. And also average weight of eggs.. Or what percentage of the engorged tick weight will be egg weight
any help will be highly appreciated
I found a species of Coniopterygidae preying on eggs and nymphs of Aleurothrixus floccosus in a lemon tree. I have followed its biology and have pictures of eggs, larvae, pupae and adults. With my thanks in advance, any help is welcome.
Luis
I was looking for any scales that help to measure the idea of Easter eggs in a marketing context.
My first female M.Sexta mated and laid eggs. After about a week she passed and her partner lived for another 20 days. After those eggs reached maturity I kept 4 females to raise to adulthood. 2 of them started laying infertile eggs and then they immediately lost the ability to fly and hold on to surfaces. One of the passed away yesterday. Why do they die so quickly after laying eggs?
Please help me if you know of any website that sells Trichoplusia ni eggs. Thank you.
Would it be possible to use an adenovirus vector in a non-mammalian species? That may sound crazy, but for example the retrovirus MLV is naturally a mouse virus, however it can transfect the eggs of the common parasitic blood fluke. Is there a way to answer that question besides looking for viral target receptor sequence homology?
We want to produce oocyst of eimeria through embryonated egg passage. We have purified and sporulated the oocyst. Now I want to excyst the oocysts and purify the sporozoites. How can I store the sporozoites also to use for further study?
1. Using IC50 values?
2. By percentage inhibition compared to the standard?
Protocol used:
After isolation: Qubit kit was used for quantification (HS): below the detectable limit
No gel-like pellet on the bottom of the tube was observed during the isolation procedure.
please give some suggetions
last week, we observed several "parasites/leech" on freshly laid zebrafish eggs. Please see the video and photo attached. Has someone seen such organism ? Can someone identify it ? and eventually knows how to get rid of it?
thanks for your help.
We need some Spodoptera littoralis for an experiment (eggs, that should hatch the same day).
The source we had so far cannot provide anymore.
I am trying to rear D. hydei on Carolina Instant Medium (in an incubator at 25C with 12:12 L:D). I have not been adding yeast. The flies are surviving but not thriving. I’m looking for recommendations to improve things.
Ultimately, I want to observe oviposition behavior. I’ve read females begin courting much earlier than males. At what age have others tested egg-laying? Males and females same age? How long to allow them to lay eggs? Since they re-mate often, should I keep the males in the oviposition chamber? With D. melanogaster, I use 4-day old females that have been kept continuously with males but I put only females in the oviposition chamber (overnight for ~15 hours). Wondering how I might need to modify this protocol for best results with hydei.
Hello everyone
I would like to know please what are the in vitro biological activities that I can test for hydroalcoholic plant extracts. Namely that I have already carried out the following analyses: - Antioxidant activity (DPPH, FRAP)
Antibacterial and antifungal activity
Anti-inflammatory activity (test of egg albumin denaturation and BSA denaturation test) Antihyperglycemic activity
Thanks in advance
We have a problem of snake predation on B type nest boxes (entrance hole diameter: 32 mm). The culprit is the Aesclupian snake (Zamenis longissimus), and they eat eggs and nestlings alike. The nest boxes are hanged on trees at c. 3-4 m height at a periurban forest. We already tried to cover the tree trunks with plastic sheets (following Navalpotro et al. 2021, DOI: https://doi.org/10.32800/abc.2021.44.0103) and also relocated the nest boxes to more stand alone trees (the canopy of which does not (too much) overlap with neighboring trees; this is not always100% as it is not easy to do so in a forest. But all in vain. It seemingly worked for a week or two but then the predation continued and the situation is the same this year. I attached some pictures that can help evaluate the situation.
So the question is: do you have any good suggestion for this problem? Perhaps to treat the plastic sheets with some smelly material?
Thank you in advance!
The data collected is on time to appearance of different developmental stages, of fish eggs in concentrations of toxicant. Five concentrations of toxicant and eight egg developmental stages were monitored (total of 40 cases). The data was ranked with the following results: the distribution of 15 variables were normally distributed (Shapiro-Wilk test) both before and after ranking using SPSS version 25 (37.5%). Twenty-one variables became normally distributed (52.5%), while two cases were not affected. I read somewhere that data normalized by ranking can be analyzed using parametric methods. Does this apply here? Thanks.
is there any way to make an egg trap for mealworms to seperate the new eggs from the beetles, i saw that it works with superworms
I've came across the fact that egg membranes are extracted by means of dissolving the calciferous shell in acetic acid, but does it truely harm the protein structure of the membrane.
Normally, fish and larval stages are preserved in 5-10% buffered formalin prior to morphological identification. If we are going to use, molecular level identification, we have to preserved them in 75% ethanol. However, in most of the recently published papers, 75% ethanol is used, even for morphological identification.
1. what is your recommendation of using ethanol for this preservation if we are going for morphological identificaton?
2. If you have literature on preservation technics of fish eggs and larvae can you share them with me?
In the discovery of mammalian egg von Baer reported to have seen a yellow fleck inside the follicles; this could correspond to sighting- with the microscopes of that time- to the cumulus o-hutus, which convex face in the antrum could be described as a fleck or point?
I bleach c. elegans worms and receive lots of eggs but they do not hatch despite using the exact same conditions that made them hatch before.
I made geriatric food using flour, lentils, caroots,spinach and eggs to test total phenolic content as total flavonoids. I dissolved my sample into methanol for extraction and then centrifuged it to get the supernatant which is then concentrated using rotatory. But the solvent is not wholely evaporating. My yeild is pasty. I couldn't calculate the percenatge of yield as it is in pasty forn not in dry weight form
Only glass tubes can be used for Diethyl ether as far as I know. But i'm not sure how safe it is to centrifuge fish eggs in diethyl ether at 10000g. This is a step in the cortisol extraction.
Paragonimiasis is a parasitic disease that can be transmitted through the consumption of infected freshwater crabs by mammals including humans. Infective larval forms of the parasite has been found in crabs but not so much adults were found in humans. Therefore, I think that the other mammals in the vicinity of the freshwater crab distribution must be responsible for the continuation of the life cycle of Paragonimus westermani. However, I need to find out if the wild mammals near freshwater bodies are infected with the parasite by examining their stools for parasite eggs. How can I do this?
These eggs (?) are quite confused to identification under the light microscope. My friend who has research project about the diet composition of Celebes halfbeak, Nomorhamphus liemi found these things in their digestive tract. We thought these are maybe insect eggs, or some microorganisms' eggs, according to the main diet of this fish is insects (larvae insects in river bodies). So, here are some photos of the "eggs", sorry for the limited photos we had.
Thank you.
Kindly explain the procedure to conduct egg hatching test and larval development test for Haemonchus
Is antibody produced against fowlpox virus vertically transferred via eggs and further inhibited viral propagation on non-SPF cells?
I want to compare the import of animals as food (meat, fish, egg, cheese, butter, animal fat and oil etc) with the other import of animals in ensuring a sustainable human life. Which is greater to human's sustainability, the nutritional value of animals or their other value?
Given a hypothetical situation where no human ate any animal foods that are important in our nutrition today, but rather ate only plant-based foods, leaving all animals to serve their other purposes in life, would humans experience a better or a worse sustainable human life (if all other ecological circumstances remain the same)?
What is your expert opinion and source of reliable data?
I have recently found a fairly slender isopod attached to the eggs/embryos of an Apseudopsis mediterraneus (Tanaidacea) sampled off the coast of Israel. The isopod itself appeared to be protected by a thin mucous sac.
Any ideas where I should start my search for a possible identification?
Many thanks.
Graham Bird
can we use egg adapted influenza virus for serum neutralization test in MDCK cells?Thanks
Hi!
I have an idea to use a chicken embryo as a model for endothelial disfunction studies. Who has already worked with the vessels of the embryo. It looks so beautifull and easily accessible for the investigation, but I can't find the nethod of the fixation the vessels just from the eggs for the morphological study. Tell me if you have any ideas too
Hi. I am a bachelor degree student and currently conducting a survey research about Knowledge, Attitude and Purchasing behaviour towards chicken egg, and willingness to pay for chicken egg. May I know what is the independent and dependent variable for this research? I'm still new with this type of research method and confused which variable is which. I really appreciate your help and thank you.
As we know that the sperms function test is widely used for the management of male factor issues worldwide. However, similar parallel test is not available for the management of egg factor issue in female. Do the number of oocytes available for the purpose or the unavailability of the potential biomarkers for oocyte quality and other factors limit the development of oocyte function test? Kindly give your expert opinion.
Well, we read a lot about how in vitro manipulation of oocytes can induce stress and therefore reduce their potential or as people call it "quality". I've been looking into the studies mostly, they focus on the oxidative stress and reactive oxygen species relation with calcium regulation. Other studies investigated the heating as a stress factor. Well, how they established that this oocyte is healthy and this one is stressed? On what basis? What kind of methods they used? Are the polscope data useful?
I am a MS student at the University of North Carolina at Charlotte and I work with ovigerous fiddler crabs and the potential temperature stresses these crabs or their embryos may be exposed to in the field. An experiment has been set up to determine what temperatures these fiddler crabs are exposed to inside of their burrows while they allow incubation of their embryos.
Does anybody know if there is any correlation between the tide and the temperature fluctuations that fiddler crabs experience inside of burrows? especially while they are incubating eggs?
Dear colleagues,
I found eggs of helminths and nematodes in feces of red fox in semi-desert territories of Shirvan National Park (Azerbaijan, Caucasus).
I believe that eggs of nematodes belongs to Strongyloides, The taxonomical status of other eggs is enigmatic for me. :-)
The photos of above-mentioned parasites is attached.
Sincerely,
Mehdi Ali.
+1
Hello every one
Please I need any articles about obtaining eggs containing immune proteins by injections hens to can use as something like a vaccine against covid-19 or any other diseases.
I am doing Iodine Test of insect's egg in flour for the first time and I just want to validate my results if what I have seen in the microscope is really the weevil's eggs.
