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Ecology and Evolution - Science topic

Evolution of new species is significantly controlled and directed by the environment the species dwells in, its niche requirements, its adaptability to the most subtle changes in its niche as well as the whole ecosystem in which its niche lies. A discussion in this forum are: behavioral ecology, marshland ecology, bio-fuels and evolution of animal societies. The focus of discussion can be broadly divided into two different direction apart the basic ecology aspects of evolution i.e. molecular evolution as well the mathematical aspect, both understanding and modelling of evolutionary ecology.
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Phylogeny analysis seems beyond my capacity right now, however, there are so much information in my dataset of seed traits along with climate, phenology, any suggestion will be appricitated.
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I am searching for good recordings of distress calls emitted by American crocodiles, in particular by hatchlings. The longer the better. If not, also distress call recordings of other crocodilian species are fine.
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Differences in distress: Variance and production of American Crocodile ( Crocodylus acutus ) distress calls in Belize
Visit to that article
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In 2016 I have published a series of recommendations for improving water quality in recirculating systems. This was published by my contractor (a public research center) in their website as an open access file (no ISSN, DOI...). https://www.juntadeandalucia.es/agriculturaypesca/ifapa/servifapa/registro-servifapa/bf663551-17f0-4c55-bbde-fe777f15da77
Eventually, this was cited in Sempere et al. (2018). Should this be counted as an "oficial" citation?
Thanks for your opinions,
Paco Sedano
Sempere-Valverde, J., Sedano, F., Megina, C., García-Gómez, J. C., & Espinosa, F. (2019). Feeding behaviour of Patella caerulea L. and P. rustica L. under spring and neap simulated tides. An innovative approach for quick quantification of grazing activity. Ethology Ecology & Evolution, 31(3), 283-292.
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Sempere et al. (2018) count as a citation. The status of "official" is given by the database one uses for retrieving the citation information. If the database used is the one of the search engine Google Scholar, your text has as of today received 6 citations. Congratulations on this achievement!
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I have a question, when I used the picante function to analyze the phylogenetic community structure and phylogenetic signals in R, it appeared an error:"'phylo' is not rooted and fully dichotomous", I don't understand what's the problem, the attached file is my phylogenetic information, please check it, I am sorry to trouble you all, but I really want to solve this problem, thanks a lot.
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Yes, i think too
*The unrooted tree should be transformed into rooted tree. Try this code, phy=multi2di(phy1), where phy1 is the unrooted tree.
Thanks Wenjie Wan
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The solution for immediate stabilization of DNA, RNA, and protein in tissues.
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Unfortunately it's proprietary.
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I would like to know if anyone knows about other software other than Genemapper/Genamarker to visualise peaks, or whether there is any online platform available? Thanks in advance.
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I do agree with mr. Rileys suggestions. Or try to collaborate with a gp that hsa the ABI tools
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Please help me in the identification of this well preserved fossil plant found in Tufa . Thank you in advance !
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Jean-Jacques Châteauneuf Thnak you very much for your comment. Best regards.
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Dear all,
I am using linear mixed effect models in my experiment (using r package). I would like to show a measurement of the fitting quality of the model. I see that there are lot of methods but two of the most frequently are:
1) Xu, R. (2003). Measuring explained variation in linear mixed effects models. Statistics in medicine, 22(22), 3527-3541.
2) Nakagawa, S., & Schielzeth, H. (2013). A general and simple method for obtaining R2 from generalized linear mixed‐effects models. Methods in Ecology and Evolution, 4(2), 133-142.
I have tried to use those aproach to my data obtaining different results. Please look:
Approach 1. Method from Nakagawa et al.(2012) basing on this post: https://ecologyforacrowdedplanet.wordpress.com/2013/08/27/r-squared-in-mixed-models-the-easy-way/
> r.squaredGLMM(reves_m1)
R2m R2c
[1,] 0.02128424 0.5280393
> 1-var(residuals(reves_m1))/(var(model.response(model.frame(reves_m1))))
[1] 0.5338557
As you can see R^2 marginal from the Nakagawa formula is very similar to the R^2 obtained with the formula of Ronghui Xu but in other post I have read that it is better to use the R^2 marginal from the Nagawaka formula. Could someone give me some idea of the differences between R^2 marginal and R^2 conditional? Which one I should comment in my paper if I would like to talk about how the model fits? In my case the R^2 conditional is moreless high (0.52) but the R^2 marginal is very low (0.02)
Thanks very much,
Gabriel Delgado
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You may want to look at the function "get_variance()" from the "insight"-package (see https://easystats.github.io/insight/ resp. https://easystats.github.io/insight/reference/get_variance.html).
This function extracts all variance-components of mixed models and supports different packages (like lme4, glmmTMB, GLMMadaptive, lme, rstanarm, afex, ...). The code is based on the latest proposals from Nakagawa et al 2017 and Johnson 2014.
There is currently a package that will be based on this functions to compute R2 and ICC (intraclass correlation coefficient) for mixed models, but you can easily compute the values by yourself for now, which will also help you understand what the R2 values mean:
m <- lmer(Reaction ~ Days + (1 + Days | Subject), data = sleepstudy)
vars <- insight::get_variance(m)
rsq.marginal <- vars$var.fixef / (vars$var.fixef + vars$var.ranef + vars$var.resid)
rsq.conditional <- (vars$var.fixef + vars$var.ranef) / (vars$var.fixef + vars$var.ranef + vars$var.resid)
So, the marginal R2 is the fixed effects variance, divided by the total variance (i.e. fixed + random + residual). This value indicates how much of the "model variance" is explained by the fixed effects part only.
The conditional R2 is the fixed+random effects variance divided by the total variance, and indicates how much of the "model variance" is explained by your "complete" model.
If you would like to know how much of the proportion of variance can be explained by the random effects only, then you have the ICC. This is simply:
icc_adjusted <- vars$var.random / (vars$var.random + vars$var.residual)
"r2()" and "icc()" are currently implemented in the "sjstats"-package, but will be re-implemented in another package ("performance"), which will be released within this month...
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The second law is generally considered as a dissipative law, whereas, many evolution processes show that order could arise spontaneously, in my opinion, the second law itself contains the mechanism of evolution.
In the fundamental equation of the second law,
diS=ΣdiSj ≥0,
here diS is the total entropy production, and diSj is the partial differential of diS in process j.
The equation does not require each of diSj≥0, it only requires that the total entropy production is positive definite, but the partial differentials of which can be less than zero. In the case diSj=k<0, order may arise spontaneously in process k.
Please see
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Yesterday I occasionally deleted my long answer to this question. Briefly.
The second law of thermodynamics is valid only for isolated systems. The Earth is not the isolated system. The origin of life does not violate the second law. Chemical reactions commonly proceed with a decrease of entropy. A reaction might proceed spontaneously if the change in free Gibb's energy is negative. If the reaction is thermodynamically favorable, this does mean that the reaction will proceed. The reaction rates rae controlled not only by thermodynamics but also by kinetics. All living organisms (as well as all organized systems) are thermodynaically unstable but stable kinetically.
Tang Suye My friendly advise. Learn chemical thermodynamics instead of writing such out of ordinary text:
"Consider a chemical reaction, -ΔG/T≥0 (ΔG≤0), the changes in the system involve the two items, one is the change in the internal energy, another one is the change in the molecular structure, the first item relates to calorimetric entropy,"
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I am interested in the phylogeny of vertebrates, i.e. the phylogenetic relationship among fishes, amphibians, reptiles, mammals and birds. I find a cool article published in 2003 (see below), and want to know the recent advances in this field.
Could you provide any more recent information on the phylogeny of vertebrates?
Thanks.
AxelMeyer, RafaelZardoya. Recent advances in the (molecular) phylogeny of vertebrates. Annual Review of Ecology, Evolution, and Systematics. 2003, 34: 311-338.
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New facts or new interpretations are presented every year. (Some of the new advances, however, soon proved to be erroneous.) As a starting point safe, I suggest a good textbook – e.g., Vertebrate life (Pearson, 2013, 9 ed.), by F H Pough et al.
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Based on my research, this method is used to assess the adequacy of sampling, but I don't know what the difference is between them.
Can anyone help me regarding this subject?
Which individual based and sample based method is better for determining the adequacy of sampling?
My study was carried out in two regions with different climates and in each region, we are sampling in two different management regimes.
Which scale (management, climate regime or total data) must be used in analysis to assess the adequacy of sampling?
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Species accumulation curves (or collector’s curves) are used to estimate the number of species (i.e. species richness) in a particular area. A species accumulation curve records the total number of species observed, during the process of data collection, as additional individuals are added to the pool of all previously observed or collected individuals or samples. Accumulation curves may be either individual-based or sample-based.
A species rarefaction curve answers the question "how many species would a smaller sample include? Say you had two samples, 'A' with 100 individuals total and those 100 individuals distributed among 9 species and sample 'B' with 25 individuals distributed among 4 species. Rarefaction answers the question "How many species would I expect in sample A if I had caught only 25 individuals in all instead of 100?" The species diversity of two samples, containing 100 and 25 individuals respectively, can be compared directly by rarefying the larger sample down to 25 individuals.
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What we'll do when after 40 years we use all global proved oil reserves? The global economy consumes approximately 1.26 trillion U.S. gallons of oil. One trillion U.S. gallons is about one cubic mile of oil (1 CMO). The world consumes ca. 3 CMO equivalent energy annually from all sources. Global proved oil reserves are estimated roughly at 43 cubic miles, or 43 CMO. After 40 years we will used all of them. What we 'll do later?
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Dear Dr. Barbara Motyka , it is an interesting issue.
Before we run out of oil, we would have moved to alternate fuels. Not because oil scarcity, but because of climate change. In the world of companies like Tesla, such a shift might not take long.
Germany has mostly moved in solar and China is fast following. India is also making some of the biggest solar plants that has dramatically brought the cost down to near the levels of coal. India's big move into solar is already paying off. In another 6 years, India alone plans to add 100 GW to the solar capacity. India Aims To Achieve Colossal Renewable Energy Targets 2 Years In Advance
Thus, on the one end we are already making great strides on producing energy without fossil fuels. The next part comes in storage and distribution. As you know, solar power doesn't come at night. People are already working on creating multiple energy sources mix to reduce this problem as well as researching a lot on batteries. Within another 10 years, Tesla powerwall and its competitors could be in all our homes. This is not hard to envision - a lot of people in India already have battery  inverters at home that store and release electricity given the unreliable supply.
Until we get there on battery storage, we might have a temporary point of time where we might be firing a lot of coal plants merely to balance load. That should be ok.
The third part comes in energy usage. Companies like Tesla are already making electric cars a reality. In parallel, there is the electrification and almost all trains would run on electricity in a decade [replacing Diesel]. A lot of metro train projects are underway in the developing world where again they replace diesel powered buses with electric powered trains, whose energy source would eventually be solar. 
While the drastic drop in oil prices have somewhat slowed the progress in clean energy, definite progress is occurring there. We are not far from a time where almost everything would run on solar, that will then be stored and used efficiently.
Based on the comments below, let address a few things.
Fact 1: We are rapidly moving into clean energy. This is the fastest shift in our energy usage since the dawn of time. Here is how Germany draws electricity through a day. Fossil [coal, natural gas, diesel] is now less than 50%. At various points of the day, solar supplies nearly half the power.  You can follow this blog - CleanTechnica [no association to me] for latest news on this.
France uses nearly nothing of fossil fuels for electricity.
Why not nuclear power?
While I'm a proponent of nuclear power, I don't think nuclear replaces the need for solar/wind for the following reasons.
  1. It is extremely capital intensive. There is not enough resources in the world to take whole world nuclear based on current technology.
  2. It is extremely slow to build. Nuclear plants take 10-15 years to complete at a minimum. However, in places like India, the energy need is today and cannot wait for 20-30 years.
  3. Nuclear plants add a lot of risks - from nuclear proliferation to earthquakes and extra protecting required during war. This risk is worth it only if cleaner sources of energy are expensive.
  4. Until we figure out a way to use Thorium or hydrogen, we are left with a depleting fuel of Uranium that is like fossil fuels - limited in availability, dirty mining methods, dirty output/sideproducts. Worse, only a few nations produce this fuel and these nations can cut off their exports in times of conflict [producing a terrible energy security problem].
How will it affect geopolitics?
That part is much more complicated.
  1. There will be massive shifts in global trade and shipping. Energy scarce countries like India will have massive boost to their trade balance. Shipping industry will have a big fall as you won't need supertankers and related infrastructure any more. Pipelines might also go away, and so might a lot of mining & oil drilling and all of those could cause major changes in infrastructure industry.
  2. Energy security worries will be a lot less. If every country is producing most of its energy from its own borders, they would worry slightly less about geopolitical games. Protecting energy sources and routes is now a big thing for the major powers.
  3. West Asia and Central Asia will lose a lot of the allure. While these would still remain a major trade route, it would only have as much importance to world as it was in early 20th century before oil was found. That might mean less wars in the long term and a lot of depopulation [most of the residents are immigrants in many gulf nations].
  4. Developed world will be a lot more assertive as they would rely less on the developing world for energy. There won't be a pesky OPEC to worry about nor Russia's energy threats or worrying about pipelines crisscrossing the globe.
It would really be an interesting world. In short, stop worrying about the stuff running out underneath us. Worry about the stuff we are filling above us.
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World's urban tales had been told many years ago that polar bears are wandering through Polish country. It was never true in historical times, however Alfred Jahn has written in his "Ice and glaciations" (PWN 1971): "In Poland, snow begins to fall mostly in December, and in January and February already covers the earth with a thin layer. This happens when the air temperature drops below 0deg, when the water freezes and the earth is covered with a hard, soggy clod. The change takes place in March. Just a few days of thaw ..." This winter we have here up to 9degC and a thin layer of snow was with breaks for... four weeks no more. In late December I've found the willow flowers at the walk. Daisies bloomed on the lawns. It's a rule now. However, it is not question in plant biology. We start to enjoy with a mediterrenian climate, now. And the mediterrenians? Now it is hard to stay there in the summer time. We also are the most calm country in the Europe with longest white-and-yellow sandy sunny beaches at the seaside. Will Poland be the best place to live for next few centuries?
This winter season the first thin layer of snow occurred here on 5th January 2020.
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In Greenland the Inuit home rule government has a policy to install hydropower in every town tapping the energy from the meltwater. So far they have build six. Each city supplied has ceased to use their diesel generator power plant.
The second photo is of the Sisimut plant close to my universities Sisimut campus.
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Dear colleagues
I am preparing MS which partly deals with relationship between trophic level and niche breath. I search literature for relevant examples but apparently miss papers that dealt with these topics; except some general speculations and quite few case studies. If you remember some papers dealing with following hypothesizes (1, 2) please give me some hints.
1.  Species of higher trophic levels are characterized by more generalized trophic habits
2. Habitat generalists are food generalists
Obviously, one can argue that there are good reasons for strong, weak or no relationship for  1,2. I search for papers showing patters and dismissing them. They could be both empirical and theoretical ones.
I appreciate any help you can provide.
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Hi Marcin,
An animal can be both a habitat generalist and a feeding specialist. I work with an endangered carrion beetle that is said to be a habitat generalist and a breeding/feeding specialist. Nicorphorus americanus (American burying beetle) buries small dead animals as a breeding and feeding resource to raise its young. (Yes, it's an insect that uses parental care) There are other Nicrophorus species around the world that are great study subjects due to their interesting life histories. The beetle is considered a habitat generalist because it isn't tied to the habitat per se, but rather is tied to what dead animals are found in that habitat. Here is a link to the beetle webpage that has all kinds of info about the little critter.
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Hi,
According to Quieros et al. 2013, Carcinus maenas falls within the bioturbation class of 'Regenerators'.
'Regenerators' are excavators that dig and continuously maintain burrows in the sediment, such as the fiddler crab Uca spp (see Kristensen et al. 2012). However, I doubt C. maenas is a typical regenerator as it does not maintain a burrow system.
My question to you is, how would you classify this species in terms of bioturbation? In my opinion it could be classified as a surficial biodiffusor as it finds most of its food in the top few cm of the sediment (even though it may occasionally dig deeper for food). I have to mention that the study site is a Dutch intertidal sandflat where we mostly find small specimens.
It would be very helpfull if you can let me know on how you think about it!
Thanks Pieter
Queirós, A. M., Birchenough, S. N. R., Bremner, J., Godbold, J. a, Parker, R. E., Romero-Ramirez, A., … Widdicombe, S. (2013). A bioturbation classification of European marine infaunal invertebrates. Ecology and Evolution, 3(11), 3958–85. https://doi.org/10.1002/ece3.769
Kristensen, E., Penha-Lopes, G., Delefosse, M., Valdemarsen, T., Quintana, C. O., & Banta, G. T. (2012). What is bioturbation? the need for a precise definition for fauna in aquatic sciences. Marine Ecology Progress Series, 446, 285–302. https://doi.org/10.3354/meps09506

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Hi Pieter
The bioturbative effects of the shore crab Carcinus maenas are limited, since many live in hard-substratum environments. But indeed, in sandy areas it shows either locomotion (small footprint but many legs!) or temporary burrowing activity. Burrowing may be either for finding food with chaelae or for hiding. In sandy areas, the shore crab may temporary hide in the sediment completely by digging in backwards. It does not dig deep as it keeps contact with the sediment surface.
This being said, following Kristensen et al. (2012), I would consider the shore crab an 'epifaunal biodiffusor'. C. maenas is not well adapted for living in and moving through the sediment like the examples mentioned for 'surficial biodiffusors' (e.g. burrowing sea urchins). Also following Kristensen et al. (2012) it cannot be considered a 'regenerator' as does not maintain burrows continuously.
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Could you give me some principles / concepts on ecology of spread of diseases? For example, deforestation causes spread of disease because their niche was destroyed. What concept would best explains this?
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Hello John Vincent!
I agree with Katrina, that among the factors that affect the spread of a disease is the population size, like in the case of parasites. In the simple equation for the number of infected hosts in parasitism (Rp), Rp = NBL (where N is the population, B is the transmission rate of disease, and L is the virulence of the disease), it is inferred that the greater populations have higher susceptibility to the disease since there are more hosts to infect.
On the genetic level, a more genetically diverse population has a higher chance of surviving an epidemic. Genetic diversity could act as a buffer in the spread of a disease. Aside from this, climate could also play a part in an epidemic as pathogens are known to thrive in warmer conditions. Hence, an increasing temperature will also increase disease in several hosts such as plants and humans.
Should you wish to find a more thorough discussion on these, you can find them here: https://www.nceas.ucsb.edu/science/disease#
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I'm doing a population genetic study on microsnail and I've found very high intraspecific genetic divergence between isolated population at very small scale. I'm curious to compare my results with similar studies on population structure of small organisms with very poor dispersal ability. Do you have any paper in mind?
It could be fresh water organisms in close-by ponds, or two population of invertebrates on the opposite sides of a river.
I know that "small scale" is relative to size and dispersal ability, but I'm curious to see what is the absolute shorter distance at which we can observe conspicuous allopatric divergence between populations in eukaryotes. 
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Hello Giacomo;
     Species of ants in which the new queens disperse by walking are good candidates for differentiating populations in the fashion that you are thinking about. Here is a reference for an example that might be of interest.
      Jowers, MJ, et. al. 2014. Recent speciation and secondary contace in endemic ants. Molecular Ecol. doi: 10.1111/mec.12749.
Regards, Jim des Lauriers
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I have the data for both morphological and reproductive traits for 22 species and the phylogenetic tree built by maximum likelihood with branch length. I need to estimate the evolution rate between these two trait categories to show whether reproductive traits evolve faster than morphological traits. Which parameter I should calculate it for comparison? dose any body have R codes for doing this kind of comparison?
Any hints or recommendations?
Thanks everyone!
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Thank you Dr. Provete for your suggestion.
All my data are continuous trait like wing length, wing width for morphological & testis size for reproductive traits. As you mentioned parameter (sigma) you mean by Variance? Please can I have your email address, I prefer to be in contact with you through email, and this is my email:
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and the best way to identify variables which used in analysis?
Ecological data : physiochemical factors ( DO,pH,....etc...) ,Time (Months, seasons...) , Stations
Statistic : CCA, PCA , DCA....
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If you have a variety of variables (time, pH,... n var.). I would recommend using the NMDS multivariate statistical technique. Because you have multiple scales in your variables. However, the statistical technique that you need to use depends on the nature of your data and the question you want to answer.
Best regards
Cristian Martínez
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This specimen of Carabus intricatus (Linnaeus, 1761) raised and lowered the abdomen alternating and spread the cerci (?). There was no other specimen present. It was in the natural environment, in 2016-05-21 in the evening, Germany, Bavaria. I noticed this about a quarter of an hour.
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Dear Rudi and Rinaldo,
these appendages are unique to female beetles and very prominent in ground beetles. If a pheromone was released, you could not have noticed. However, up to now no sex pheromone is known for ground beetles although it supposedly should exist.
In the past I have also seen female ground beetles with extracted genitalia as seen in your photos after an interrupted copula or if the specimens underwent pressure. So there may actually be different explanations ...
Best,
Fabian
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I think to use statistical tools of niche modeling like Environmental Niche  Factor Analysis or PCA-Env to analyse niche of species not studied in certain part of distribution range, but is this relevant enough to be published? if yes what kind of article? 
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Mokrane, What is your definition of niche? What is your challenge according to the niche concepts?
Firstly you should specify the niche concepts and niche dimensions in your study. For example, ¿Are you interested in the fundamental niche or the realized niche? This is a much-debated issue in the bibliography. Here, I suggest you several references. Good luck!
References: 
Barve, N., Barve, V., Jiménez-Valverde, A., Lira-Noriega, A., Maher, S. P., Peterson, A. T., … Villalobos, F. (2011). The crucial role of the accessible area in ecological niche modeling and species distribution modeling. Ecological Modelling, 222(11), 1810–1819.
McInerny, G. J., & Etienne, R. S. (2012). Stitch the niche - a practical philosophy and visual schematic for the niche concept. Journal of Biogeography, 39(12), 2103–2111.
Peterson, A. T., Soberón, J., Pearson, R. G., Anderson, R. P., Martínez-Meyer, E., Nakamura, M., & Bastos Araujo, M. (2011). Ecological niches and geographic distributions (Vol. 49). Princeton, NJ: Princeton University Press.
Pulliam, H. R. (2000). On the relationship between niche and distribution. Ecology Letters, 3(4), 349–361. 
Soberón, J., & Townsend Peterson, A. (2005). Interpretation of Models of Fundamental Ecological Niches and Species’ Distributional Areas. Biodiversity Informatics, 2, 1–10.
Soberón, J. (2007). Grinnellian and Eltonian niches and geographic distributions of species. Ecology Letters, 10(12), 1115–1123. 
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The salinity of the dead sea is too great to support life. The freshwater rivers that run into the dead sea are therefore ecologically isolated. How is it that river crabs occur in these locations. Could it's eggs have been transferred by the wind, or was there a time when the dead sea was at a higher level, and was therefore less saline?
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No one seems to have noticed that Potamon potamios is a semi-terrestrial crab. Its areal is whole of Eastern Mediterranean and it requires neither continuous water paths, nor birds for its dispersal.
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Neutral theory suggests that most  of the genetic variation in the population is the result of mutation and genetic drift. But what are the implications for interpreting pattterns of genetic variation?
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Dear Ellynn,
The recent and very interesting paper by Fujisawa et al. (2014) would be helpful in this regard:
"Ecology has contrasting effects on genetic variation within species versus rates of molecular evolution across species in water beetles"  
Best wishes,
Temim
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In addition, as a new species emerges, what happens to the population of the previous species? Do they evolve into new species as well or competition with the emergent species causes their extinction? And if the emerging species did interbreed with the existing species, could that affect the speciation process?
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Hi all,
New developments this week:
DNA hints at earlier human exodus from Africa
All non-Africans alive today likely descend from people within a single migration out of Africa
Genetic ‘trace’ in Papuan genomes suggests two expansions out of Africa
The first genomic history of Australia’s peopling
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Which category would you put Chionanthus africanus in (between pioneer or climax species, in other words, could it be an early succession or late succession species?
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Hello again,
I would like to ask Agevi Humphrey if you have a reference for the successional status of Chionanthus africanus as a late successional sp. would you please share it with me. Thank you
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Does the Ampullariid snail Lanistes show indeterminate growth. I find there is very limited biological and ecological information available on this genus
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Hey Ranjeev,  Good question - and I bet that nobody really knows!  The problem arises that because of the often severe Ca limits to growth, the snails are probably constrained most of their short lives - so it might appear as indeterminate in a Ca-rich habitat, or determinate in a less Ca-rich environment.  Sorry that I cannot provide an easy answer - but that is the nature of the beast!  Good luck! 
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I am looking for African passerine nests, specially in American collections.
Weaverbird nests in particular. Thanks!
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Having done similar searches myself, I can give you the advice that helped me the most:  Simply write to the curators directly and individually.  This will only be 15-20 emails and you will get to know - professionally - the curatorial staff and manner of each museum and/or collection manager.  In addition, nests are often not fully online yet, so you might get the best results by reaching out to each ornithology department and the people rather than trying the searches online.  Good luck!
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It is widely accepted that the major factor affecting the evolutionary optimization of animal life histories is energy balance, therefore studies focus on the energy costs and benefits of adaptations, the efficiency of energy acquisition and investment, and limits to energy budgets. However, at the very least in heterotrophs, equally important seems to be the problem of maintaining stoichiometric balance.
There are two approaches in eco-evo studies that consider the matter balance as complementary to the energy balance: ecological stoichiometry and nutritional geometry. However, in my opinion, such studies are limited and after 30 years after Tilman's and Reiners' works (below), still "energocentric" point of view dominates in ecology and evolution, that carelessly underrates the need to balance the diet also in terms of the matter (including the Law of Conservation of Mass).
This is only my point of view, possibly the wrong one. I would like to ask all of you: what is your opinion?
My question was introduced as briefly as possible, don't hesitate to dig deeper and extend it!
Below I present four important studies related to the topic, just to start with.
Kind regards,
Michał Filipiak
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Hi,
The question is how to integrate the stoichiometric concepts into the field data recalculations calculation ? Now field hydrobiologist can use coefficients of tempreture dependend functional indices like respiration or production, and amadements on oxygen concentrtation. But how to calculate disbalance or lack???
Andrey
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Dear all,
For discussion: To what extent the ecological change could generate a substantial adaptive evolution on a short time scales (20 to 30 years or within few decades)? 
with kind regards,
Dadamouny 
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Short-lived species can go through hundreds or thousands of generations in that time period, which is ample time for substantial genetic change IF there is pre-existing genetic variation that can be selected for and against. If there isn't, evolutionary change would have to rely on mutation, and would be several orders of magnitude slower. Long-lived trees may go through no generations in 20-30 years, so cannot evolve, but even a single generation can lead to substantial genetic change if there is a high mortality, strong selection and variation for it to act on. Epigenetic variation can also be heritable, at least in plants, and may be an important source of adaptive capacity in long-lived species (or may not - the evidence is currently unclear!).
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Has anyone ever tried to rear bee larvae using pollen diluted with alpha-cellulose? It has been used previously to manipulate the quality of pollen for collection by foraging bees, but I wondered if it would harm larvae to consume this diluted pollen, since alpha-cellulose is typically described as 'indigestable'?
Many thanks in advance!
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It will survive but it will be relatively small. You need a preliminary study designed especially for your research question, to check exact ratio of pollen:cellulose that you want to use. And remember that pollen itself may show variability in nutritional quality.
Best!
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Dear Colleagues,
For what I understand, to be considered invasive species, a species has to displace one or more native species. However, I have seen some publications about invasive species, where the mentioned invasive species have not displaced any native one. Am I missing something here?
Thanks,
Gloria Arratia
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Hi.
About invasive plants may be good to read this important reference, which link is:
Sometimes there is confusion with the concepts "invasive", "naturalized", etc.
Best regards. 
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We discussed in class how the Galapagos finches have developed different beak sizes due to environmental conditions (sympatry, allopatry, available food resources), and gave rise to Geospiza fuliginosa, G. fortis, and G. magnirostris (small-, medium-, and large-beaked in order). However, how can one classify which species one belongs to if the determining factor is a polygenic trait such as beak size?
We have also defined "species" as a group of similar organisms who can interbreed and produce fertile offspring. Does this mean that small finches cannot mate with medium finches and produce fertile offspring? If yes, why is this so? Aside from changes in beak size due to adaptation, what genetic changes have occurred such that they cannot be considered to be of the same species?
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So you are asking how to tell when speciation is "complete"? This is actually a very difficult question, and one for which we don't really have a generalizable answer yet. The definition for "species" is not quite as simple as the one you gave. What you refer to is the "biological species concept", which defines species as discrete units which are reproductively isolated from other such units... And there is much debate surrounding the application of various species concepts (see attached commentary by John Avise).
One problem with answering the question you have posed, is that speciation itself is a continuous process, where the parameters used to define its "progress" vary along a gradient.. For example various types of reproductive isolation (extrinsic vs. intrinsic, premating vs postmating, etc) can exist to varying degrees between populations, or "subspecies", or recognized species, or even members of different genera. We can see examples of hybridization (thus "incomplete reproductive isolation") throughout nature- does this mean that these are not "true species"?
So, along this continuum, diverging populations accumulate mutations, and accumulate reproductive isolation, either as a result of allopatry+time (geographic separation), or via divergent selection (and the cumulative effect of many other processes). Things are more complicated when we consider that the net effects of these processes are heterogeneous throughout the genome- thus the "amount" of divergence will vary depending on which locus we assay... Attempting to delineate classifications (such as "species" or "sub-species") along this continuum requires that we either treat it as discrete, or determine some arbitrary threshold at which we consider speciation to have progressed (for example, we might say "2% sequence divergence at X locus constitutes species-level divergence).. I'm not sure how far you have made it in your class, but probably you can think of many ways that this sort of attempt to quantify speciation progress might not be generalizable. I've attached another paper by Sara Via which might be interesting to read.
TL;DR- You really can't quantitatively determine when speciation is "complete", because the term "species" (and other various classifications of biodiversity) are human constructs which attempt to apply discrete delineations on a continuous process.
Just my take though
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Communication involves multiple individuals (at least a sender and a receiver). On the other hand natural selection will act based on the fitness value of communication for each individual involved in the communication process. In such case, when should selection affect on the receiver, and when will it act on the sender? (edited)
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It is not necessary that sender and receiver both benefit from a new communication process. Many signals arise by 'exploiting' the sensory system of the receiver. For example, guppy males attract more attention by females when their color matches that of the most preferred food of the females. This way, males can evolve a certain color that later plays an important role in sexual selection. The color trait (it depends on your definition from which point onward you call it a 'signal') may spread even if mating with colored males would be disadvantageous for females. A recent review article on this 'sensory exploitation hypothesis' is Ter Hofstede et al. (2015) in Current Biology.
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This photo is taken in the region of Azilal, Morocco, where the author lives. It has almost all the branches of plants in a photo.
plant evolution is not a fact?
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Abderrazak:
Brilliant photos, but why do you think that all the plant groups do not support Evolution? Whether Animal or Plant , primitive and evolved forms can co-exist at a given point of time but in geological past several groups of Life forms simply became extinct. You need to study some basic books dealing with the theory of Evolution.
Best
Syed
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I am building a sheet with morphological data to run evolutionary tests with New World Astragalus.
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Guilherme:
This link would provide some insight you are looking for.
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Syed
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Mutualism and Protocooperation are ecological interactions in which there is advantage for both partners. The first can be mandatory at least for one species, the second no. 
Among vascular plants, facilitation (or comensalism) is well-known.
However, is there true possibility of Mutualism and Protocooperation between two vascular plant species? 
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There was a previous thread on ResearchGate on this topic:
The answers were not particularly illuminating, but perhaps you could open a conversation with Guido Lingua.
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I'm looking for case studies of biological observations in floodplains that could follow the Intermediate Disturbance Hypothesis. The study noted below seems to be one of the only ones, but I'm guessing there should be similar observations looking at biota.
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Dear Mauricio
Budke, J. C., Jarenkow, J. A., & de Oliveira-Filho, A. T. (2010). Intermediary disturbance increases tree diversity in riverine forest of southern Brazil. Biodiversity and Conservation, 19(8), 2371-2387.
Montero, J. C., Piedade, M. T. F., & Wittmann, F. (2014). Floristic variation across 600 km of inundation forests (Igapó) along the Negro River, Central Amazonia. Hydrobiologia, 729(1), 229-246.
Rosales, J., Blanco-Belmonte, L., & Bradley, C. (2008). Hydrogeomorphological and ecological interactions in tropical floodplains: The significance of confluence zones in the Orinoco Basin, Venezuela. Hydroecology and Ecohydrology: Past, Present and Future, 295-316.
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How many classes were there in the world at the beginning of Cambrian explosion?
I know how many phyla were there at the beginning of the Cambrian explosion. But for the classes, I have no ideal. Could someone tell me about that and origin of data?
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This way of posing the question is wrong. There were no classes then because classes do not exist in the real world. Classes are just concepts used by taxonomists to communicate about the organisms and taxa, not "objects" or "individuals".
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Huxley's modification of Wallace's line separates Palawan from eastern Philippines. Kano's line demarcates Taiwan and Ryukyus from northern Philippines. The connection between these Kano's and Huxley's has not been resolved.
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Kano' s line? What and where
?
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For more than half of the time since the earth has been in existence, there was no oxygen. Many bacteria survived without oxygen. However that isn't my concern. I'm concerned with how this was determined. Can someone help me out?
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In addition to the useful comments above, I guess you can link it back to extremophiles phylogenetics. That will give substantial support to how basic "organism" strive in that environment, and thus, validate that theory of earth without oxygen is logical.
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When we do studies in ecology, what is our ultimate goal beyond the immediate goals of answering questions the paper?  What are we as a discipline attempting to work towards?  Note: I am asking what the discipline is moving towards, not motivations or goals of individual researchers such as tenure, renown, etc. 
Please also include your status (graduate student, undergrad, professor, industry, etc) and the decade in which you received your highest degree. 
Full disclosure: This question is part of an informal survey I am completing and your answer may be incorporated into future work exploring this question.
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 I have been reading the various answers with great interest, trying to form an opinion. Now that I have started writing, I hope my “opinion” does not turn into a rant, and my apologies if you see it as such.
 I agree, that the inclusion of human activity is a must in ecological studies these days. The study of social-ecological systems was (re)born from this need.
 Where are we going with ecological research? I would like to pose a number of questions to answer that...
How much of our ecological research is put into actual practice to improve conservation, environmental management etc.? Research grant applications may require a statement of how important the research is in practice, but how many of us actually publish our findings in a “farmer's weekly”? Farmers in Africa and Australia (and probably all over the world) are the primary owners / managers of the land. How well do we inform them? Universities don't give much credit to such “popular” publication. You need to publish in journals with the highest possible ISI rating – generally read by other academics and post-graduates who can afford the subscription (undergraduates just copy and paste the contents but don't read it). The openAccess policies that are being implemented now may help with the dissemination of information. All we need to do then is to get the farmers to read such journal articles in the hope that they understand our jargon… Until then, many of us are doing research because it's fun – in my case this is probably even more true, as I am not working in my field at the moment. I do research in my spare time.
 How many long-term ecological studies do we see? With much of our research done by post-graduates we are looking generally at 2yrs for an M.Sc. and 3yr for a PhD – maybe double that for part-time students. Students and/or universities are penalised for taking much longer than that. What are six years (-1 year for project proposal, analysis and write-up) for a forestry ecological study? Or in an arid system where the rainfall regimes dictate outcomes? Working with a series of post-docs? How many agencies are prepared to fund 50yr studies in ecology? Will you start a project (like the foresters of old) that you will never see the end of? Space-for-time approaches can only be taken so far… Do we have the leisure / time to do long-term studies? Ecological problems generally need a fast management response … make that “...fast, appropriate management response”.
Basically I see our research as part of a system that follows the instant everything approach – instant messages, fast food, instant pudding - but sadly a “just add water” approach will not work in ecological studies (no pun intended for arid systems ecologists). Universities are required to “perform” but we can't agree on how to measure performance in the first place. As a result, we have taken money as an easy indicator (grants received, student fees and government support per student etc) so we are now focussing on counting beans rather than on … whatever it is that we are supposed to focus on.
That might be topic of another RG question?
 So in answer to the original question… at the moment we seem to be building up an enormous knowledge base that needs to be tapped a lot more.
Regards to you all,
PS - Dr. in forest management 2005, 14 years of teaching and research.
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Is there a measure more appropriate than others?
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Thank you Hamdy, Marcelo has already uploaded those papers.
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I am looking for bibliographical references on the first record of a member of the Order Carnivora in South America (also including marine forms such as proto-pinnipeds, pinnipeds, otters, etc).
All your help is welcome.
Thanks in advance
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As far as I remember, the first record of terrestrial carnivorans in South America should be some Procyonidae, possibly as early as 6 Ma, and Mustelidae during the Miocene.
See those references and herein.
Cheers!
Antoine
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Please send me the link on distribution map of Brown trout (Salmo trutta) and other related species, native for the Europe and Asia.
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Hi Oleksandr,
you might want to check out AquaMaps:
There you can search for distribution maps for several fish species.
Hope that helps you getting started,
Michael
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Example of a case study : When the "Grey wolf" was reintroduced into the Greater Yellowstone Ecosystem in 1995, there was only one beaver colony in the park, said Doug Smith, a wildlife biologist in charge of the Yellowstone Wolf Project. Today, the park is home to nine beaver colonies, with the promise of more to come, as the reintroduction of wolves continues to astonish biologists with a ripple of direct and indirect consequences throughout the ecosystem. Cases of how the re-introduction of wolves , changed the river patterns significantly, is also a remarkable study!
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Just a word of caution on terminology:
Reintroductions and Introductions are two very different issues: the former indicates the release of a taxon to an area where it was present in earlier - historical - times and later got extinct, whereas the latter indicates the release of an alien (allochthonous) taxon to a new area. The release of wolves to Yellowstone has been a reintroduction, but the release of South American coypus and North American grey squirrels to Europe or that of red foxes to Australia have been introductions. The former are positive operations - if properly conducted - and tend to restore pristine zoocoenoses, whereas the latter are quite negative operations as they tend to upset ecosystems.
In both cases you should expect consequences on the ecosystems, usually favourable in the first case, nearly always negative in the second case. The effects of weather and climate integrate with these consequences (or rather the other way round!).
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I observed an interesting phenomenon in the Rain Forest of Western Ghat, India. The male fly usually waits, for a long time, sometimes for hours, inside the flower and it defends its little territory against other males, actively. When a female fly comes in, it starts its elaborate dancing. I read a bit about Hawaiian Drosophila's mating behavior, where they defend leaf surface (if I am not wrong). But I never came across any literature where insects are using flower as a site for male display and competition     (lek). It poses interesting questions like "how long the male should wait inside a flower?" (If it waits too long in the "wrong" flower, then it will be in "trouble" and if it is too impatient then also, I guess. So there should be an optimum). Does anybody have any suggestion, information, thought or reference to share?
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The D. elegans is a digital photo by my field assistant Takao in Iriomotejima.
I've collected the specimens and identified them.  I'm an entomolgist with emphasis on drosophilidae.
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Is it possible that we might have miscalculated species richness in Lepidoptera and indeed other orders if some species exhibit polyphenisms in wing colouration and patterning?
I do realize this works on the assumption that wing colouration and wing patterning are all thats required to positively ID lepidopterans however running DNA tests on all of the butterflies you catch would surely be too expensive.
Do we instead have to rely on anatomical features for positive ID in butterflies that exhibit the type of polyphenism I am talking about?
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Reliabilities of estimates of species richness for a given fauna are directly related to the completeness of taxonomic understanding of the group being counted.  For butterflies, many polymorphic taxa were recognized as such by the early 20th Century (see, for example, GAK Marshall's discussion of Heliconius in Trans. Ent. Soc. 1908).  There may be overestimates due to polymorphisms and/or synonymic taxonomic descriptions, or there may be underestimates because there are a lot of undescribed taxa.
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I'm having 3 species that I have to estimate the relative fitness with only binary survival data. I was thinking about using a fitness function taken from a GAM by applying a smoothing factor on the PC1 and PC2 of 3 traits. 
Do I have to compute the GAM with all species in the model? 
Do I have to compute the model with species in covariate? 
Do I have to compute the model and add species as a covariate? 
Will all species have the same scale? 
Is the fitness function estimated from the GAM relative fitness for a particular trait? 
Thanks,
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Hi Marc-Olivier, selection acts on traits of individual species, so you need a model for each species separately. If your response variable and phenotypic traits are the same, you may probably get results in the same scale. After you fit the models, you can compute selection gradients and check for significance using the R package gsg. Also check for the work of Morrissey & Sakrejda (2013) "Unification of regression-based methods for the analysis of natural selection". Hope this helps
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If the reproductive fitness of a parasite remained 50-60% of its total life span, then what would be possible reasons for its remaining developments? It this an example of evolutionary selection?
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Our article published in peer-reviewed Journal "Communicative & Integrative Biology". A few major points discussed in the paper:
(1) Brain is not the source of consciousness.
(2) Consciousness is ubiquitous in all living organisms, starting from bacteria to human beings.
(3) The individual cells in the multicellular organisms are also individually cognitive entities.
(4) Proposals like “artificial life”, “artificial intelligence”, “sentient machines” and so on are only fairytales because no designer can produce an artifact with the properties like internal teleology (Naturzweck) and formative force (bildende Kraft).
(5) The material origin of life and objective evolution are only misconceptions that biologists must overcome.
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I am planning a research project on the interactions between 'hedgehog galls' and bur oak (Quercus macrocarpa) in southeastern Québec (Canada).
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Try contact Dr. Spungis in Riga or Dr. Mamaev in Moscow - these are leading taxonomists for gall midges and may suggest something useful 
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I am searching for good song recordings of African Reed Warblers. The longer the better. Or maybe somebody works near the habitat of these birds and it's not a big deal to record several minutes for me :)
There are some recordings on xeno-canto.org, but they are way too short for the analysis :(
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In Yellowhammer Dialects project, I am exploiting many sources of recordings:) Surprisingly, some yellowhammer song are also on Flicker, however I bet this is not the case of African warblers.
The best would be to contact British library - they have about 47 songs: http://cadensa.bl.uk/uhtbin/cgisirsi/?ps=jvO7LMNQhB/WORKS-FILE/325200049/123 I will send you a contact to a responsible person through email, Nika:-)
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Hi. I'm looking to add some photographs to a publication I am doing on the Rodrigues fruit bat (Pteropus rodricensis) for the journal Mammalian Species.  I need a photo of a live animal, plus a photo/illlustration of the skull-mandible (see attached file for an example).  The specifications for the skull plates are fairly strict (but see image):
SKULL PLATE.  Illustrations of the skull (dorsal, ventral, and lateral views) and lateral view of the mandible must be included.  These either can be good quality images or line drawings.  Do not include a scale bar.  In the figure legend, indicate age and sex of specimen, the collection locality, full name of museum where specimen is on deposit, catalog number of specimen, and greatest length of skull or a similar measure.  A statement including the origin of the photographs or name of illustrator (if other than the authors) and permission for use must be included.  All views must be exactly to the same scale. 
Can anyone help?  Thanks.
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Hi John!
Unfortunately my photos of Pteropus rodricensis are really of poor quality (and I do not have any of the skull). I suggest to ctc 
Mauritian Wildlife Foundation
Grannum Road
Vacoas
Mauritius
Phone Number (230) 697-6097
Fax (230) 697-6512
or
Mauritian Wildlife Foundation
Forestry Quarters
Solitude
Rodrigues
Phone Number (230) 831-4558
Fax (230) 831-4559
or
Mauritius Natural History Museum
under Mauritius Museums Council
All the best.
Marco
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I want to use Moran I index for spatial autocorrelation. My input files are two matrices; (1) distance matrice of individuals based from a UPGMA tree and (2) Geographic distances between individuals
So I have two questions
(1) since I have two matrices, do I place them in one input file or separate input files?
(2) Whenever I load it up in SPAGEDI, it always produce an error message on how I formatted the input file. I followed the user manual for inputting matrices in SPAGEDI however it still gives me an error message.
I hope you can help me on this endeavor. Thanks!!
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Hi,
If you have already matrices of spatial and genetic distances, SPAGeDi is not really useful to analyse them. You could make a Mantel test or compute average genetic distances for a set of spatial distance intervals using for example scripts in R. SPAGeDi would be useful if you have raw genetic data of individuals (with spatial coordinates) from which you'd like to compute genetic distances.
all the best,
Olivier
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Habitat templet theory predicts that species inhabiting similar environments converge on their traits.
Competition however has an opposite effect, and communities structured by competition are expected to present trait overdispersion.
Resource limitations are likely to increase competition.
Resource availability is expected to comply with the number of species and the possible strategies. At least this would be true if comparing equally favorable habitats and with similar evolutionary times and regimes of disturbance.
I’d expect that species niche partitioning takes place on abundant resources prior than on rare ones.
I failed to find any work relating habitat templet and resource availability over evolutionary times and their effects on niche partitioning.
Could you please suggest me some references on the topic?
Thanks in advance
Alex
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Sorry. of course, I would say "Synusien 2. Grades".
To compensate my mistake a little, I found in intgernet a publication about a particular synusie structure in plants. Unfortunately, it is in Russian but with English summary
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Some Orient genotypes of Brassica rapa have so called A-type seed coat. Large cells of outer layer with walls made of polymer are swelling in water. The seed coat is formed by mother plant but A-type is controlled by genes in embryo.
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It has been studied recently in arabidopsis plant - seed coat mucilage secretory cell devel
opment. 
1. Windsor JB, Symonds VV, Mendenhall J, Lloyd AM.
2000.Arabidopsis seed coat development: morphological differenti
ation of the outer integument. Plant Journal22: 483493.
2. Western TL. 2012.The sticky tale of seed coat mucilages: produc
tion, genetics, and role in seed germination and dispersal.Seed
Science Research22: 1 25.
3. Yang X, Baskin J, Baskin C, Huang Z. 2012b. More than just a coat
ing: ecological importance, taxonomic occurrence and phylogenetic
relationships of seed coat mucilage.Perspectives in Plant Ecology,
Evolution and Systematics14: 434 442.
4. Western TL, Skinner DJ, Haughn GW. 2000. Differentiation of
mucilage secretory cells of the Arabidopsis seed coat.Plant
Physiology122: 345 356.
5. Western TL, Burn J, Tan WL,et al. 2001. Isolation and characteriza
tion of mutants defective in seed coat mucilage secretory cell devel
opment in Arabidopsis.Plant Physiology127: 9981011.
6. North HM, Berger A, SaezAguayo S, Ralet MC. Understanding
polysaccharide production and properties using seed coat mutants:
future perspectives for the exploitation of natural variants. Ann Bot.
2014 Oct;114(6):125163. doi: 10.1093/aob/mcu011.
7. Western TL, Young DS, Dean GH, Tan WL, Samuels AL, Haughn
GW. 2004. MUCILAGEMODIFIED4 encodes a putative pectin
biosynthetic enzyme developmentally regulated by APETALA2,
TRANSPARENT TESTA GLABRA1, and GLABRA2 in the Arabidopsis
seed coat. Plant Physiology 134: 296306.
8. Zou HF, Zhang YQ, Wei W, Chen HW, Song QX, Liu YF, Zhao MY,
Wang F, Zhang BC, Lin Q, Zhang WK, Ma B, Zhou YH, Zhang JS,
Chen SY. The transcription factor AtDOF4.2 regulates shoot branch
ing and seed coat formation in Arabidopsis. Biochem J. 2013 Jan
15;449(2):37388. doi: 10.1042/BJ20110060.
9. Esfandiari E, Jin Z, Abdeen A, Griffiths JS, Western TL, Haughn GW.
Identification and analysis of an outerseedcoatspecific promoter
from Arabidopsis thaliana. Plant Mol Biol. 2013 Jan;81(12):93104.
doi:10.1007/s1110301299840.
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Primate fecal samples stored in formalin (for later analyses of parasites) and kept at room temperature (in tropical areas).
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Hi David - if it was stored in EtOH or another OH for long periods of time at RT or frozen, you have already started the extraction process. As long as that time of 'extraction' is the same, none of the fluid is lost, and the subsequent extraction method is the same, you might be OK. (though, in other words, I do not think that samples stored in EtOH at RT are an ideal way to store fecal samples for subsequent extraction of hormone metabolites).
For formalin, I am not sure because it isn't clear to me if the metabolites will start to be extracted from the feces. There is also the issue of microbial activity in the feces, which is a problem if at RT but perhaps not if the samples stored in formalin are thoroughly mixed up in formalin. Coming to think of it, we have meerkat feces that were split in half and stored either as a) immediately frozen or b) stored in formalin at RT (for parasite work). It could be possible to assay fecal Cort metabolites to assess differences.
If freezing is impossible in the field, there are other methods to preserve the metabolites without a freezer rather than storing in EtOH or formalin at RT. See Jacinta Beehner's work in geladas and other primate species. She directly extracts the metabolites in the field and stores them on cartridges at RT and found that to be as good as directly freezing.
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I'm studying moth's community structure in several habitats of the Balearic Islands (Western Mediterranean), and it is a shame not to get major information and benefits of research for most of our captures. We are creating a moth's collection which is completely available for scientific purposes (not for collecting).
If anyone is interested please send me a private e-mail.
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Under the CFI/ERF Australian Govt. programs, its not very clear what will be the tentative (baseline) price of C for abatement of CO2 emissions or for sequestration of C in the near future. Any idea would be helpful.
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Benton is right. The ERF auction is intended to be 'blind' - that is, no-one will know the results. The government has stated that its chief criterion for awarding acceptable bids is PRICE - that is, the lower the prices for winning bids, the larger the number of tonnes of abatement it can buy for its budget. It may well be that, within the additionality rules, bidders with projects which can afford to invest for very low carbon prices (sub $10/tonne, I suspect) will be the successful ones. There have been no auctions as yet.  Regard, Bill (Sydney)
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We are studying dispersal in Spanish moss using genetic markers and we need a ("safe") way to retrieve samples from up in the canopy. Obviously, we can get the low-lying stuff (via ladder and extension trimmer), but we are struggling to get samples that are higher than ~10 m. I've heard of some people using ropes, but only on TV, and I am not sure how to get trained to do this sort of thing, and whether it is safe for some of the trees. Any ideas or citations are welcome.
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I’ve spent enough time climbing trees to know that if you want samples, there’s usually a quicker way than climbing the tree (though few are as much fun). I’d agree that the shotgun method is probably the fastest and easiest. As I don’t have access to a shotgun or a marksman, I’d resort to my bigshot catapult & a throw bag/line:
It takes a little practice to get the aim right (I recommend practicing with overripe fruit rather than stones) but you can get a line over the branch you’re interested in very easily, even at 50m high. The trick is to know that you need the string to be 3x longer than the height of the branch. Once you get the line in approximately the right place, attach a square of carpet to the string and pull that over the branch to rub off the tillandsia & other epiphytes. Alternatively, you could tie in one of those wire saws (eg. http://www.amazon.co.uk/BCB-CM020A-Commando-Lightweight-Wrist/dp/B002C3PPEE) and sample the entire branch.
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It would be good to know whether protocols of measuring telomere length in vertebrates are also suitable for measuring telomeres in vertebrates.
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Read a review "Are Drosophila telomeres an exception or the rule? by
Edward J Louis
Genome Biology 2002; and can be found online at: http://genomebiology.com/2002/3/10/reviews/0007
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Paulownia description.
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Hi
General information about Royal paulownia (Paulownia tomentosa) Tree:
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Is DNA sequence information sufficient by itself to solve this problem?
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A Reading List on the “Ancient Passerine” Proposal:
1. Schizocerate (“split horn”) finding of a doubled spinal cord dorsal horn in many modern bird families, but not in passerines, paleognaths, rails cranes and pigeons, which all share the leiocerate (normal spinal cord with one dorsal horn) condition found in all other amniotes.
Woodbury, Jeffery. C. 1998 Two spinal cords in birds: novel insights into early avian evolution. 
Proc. R. Soc. Lond. B 265: 1721-1729, doi: 10.1098/rspb.0494
2. Molecular studies showing that passerines are found at the root of the avian tree, with paleognaths. All the “cherry-picked” studies below agree on this, except iii, where passerines are still basal, but are usurped from the earliest branch by the Galloanserines, an artificial assemblage of two otherwise unrelated groups of birds (galliforms and anseriforms) that appear to be ancient only by virtue of shared, extraordinarily high, mutation rate.
i. MINDELL, D. P., M. D. SORENSON, D. E. DIMCHEFF, M. HASEGAWA, J. C. AST, AND T. YURI. 1999. Interor- dinal relationships of birds and other reptiles based on whole mitochondrial genomes. Syst. Biol. 48:138– 152. The basal position of passerines in this study was not generally accepted at first; “long branch attraction” was thought to be responsible for the heterodox phylogeny of passerines.
ii. JOHNSON KP (2001) Taxon Sampling and the Phylogenetic Position of Passeriformes: Evidence from 916 Avian Cytochrome b Sequences. Syst. Biol. 50(1):128–136 This study has a very large data set compared with i. and convincingly despatches the idea that “long branch attraction” accounts for the basal position of passerines.
iii. Kerryn E. Slack a,b, Frédéric Delsuc a,1, Patricia A. Mclenachan a, Ulfur Arnason b, David Penny (2007) Molecular Phylogenetics and Evolution 42: 1–13 Resolving the root of the avian mitogenomic tree by breaking up long branches. These authors are expert at correcting for biases in DNA that can influence phylogenetic reconstruction such as base composition and taxon sampling, but they have not been completely successful at correcting for large, circular, variations in mutation rate. For this reason, the basal position of passerines is confirmed, even lying outside the paleognaths, but the Galloanserines, with a very large rate anomaly, intrude (see 2 above).
iv. Slack KE, Jankea A, Penny D., Arnason U. (2003) Two new avian mitochondrial genomes (penguin and goose) and a summary of bird and reptile mitogenomic features. Gene 302 (2003) 43–52 In this study, Galloanserines are more appropriately placed than iii, but are still outside the paleognaths, outside of which are the passerines, in keeping with the present thesis. A nice feature of this study is the use of non-avian amniotes as outgroups. Passerines prove to be the closest sister group to the non-avian amniotes in their cladogram.
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I have struggled to do this with Powerpoint, but am sure that there must be simpler, better, more efficient software programmes for this purpose. Any suggestions? How have others done this? This is a good examples: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3146880/pdf/1471-2148-11-172.pdf
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I've found an interesting software, developed by Jessica W. Leigh: popART (http://popart.otago.ac.nz/index.shtml).
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Sadly, a number of journals seem to have done away with the option of publishing short commentaries or insights, unless they are a direct response to a paper published recently in a journal. Our lab has been generating some interesting ideas about the current ebola epidemic and ways forward in understanding the natural history of ebola viruses and are looking for a venue with which to share them with the scientific community.
So far we have found the following potential options but any additional suggestions would be greatly appreciated:
Emerging infectious diseases - Commentaries, but need to be invited,
EcoHealth - Forum
Trends in Ecology & Evolution - Letter
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May be this blog will help : http://blog.f1000research.com/
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Samples or issues from Afghan Pika for a genetic analysis
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Good morning,
I write from Argentina, I am a dendrochronologist working on semiarid environments. I do not have the samples that you are asking for but do not hesitate to contact me for any other question that you have.
Best wishes.
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I want to do some interspecies comparative research. And I'd like to run PGLS in R. But I've got a tree with out branch lengths. The common ways to run PGLS need a tree which includes branch lengths. Then how shall I do it with a tree without branch lengths? How shall I set the branch lengths to make it adequately statistic? what's the detailed codes? 
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I agree with Jan that setting branch lengths equal to 1 is a good starting point if you don't have a robust phylogeny. If you have some idea of the relationships between your species then you can make your own informed tree with branch lengths set at one, but it is nearly always going to be advantageous to build a tree using molecular / morphological data if you can. GenBank has many sequences available that you may be able to use, and there are various R packages that allow you to trim down trees so that you can exclude unwanted species from published phylogenies. Hope that helps a bit
Good luck
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I would like a key to distinguish all the taxa, or at least those closely related to F. hexapetala. Phylogeography and phylogenetic studies would be interesting too.
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Gbolade AA, Olayemi JO, Elujoba AA, Sofowora A, Adesina SK. 1992 Factors affecting the levels of steroidal sapogenins in Nigerian Agave and Furcraea species Fitoterapia 63. 45-48. (EBBD, 190204324).
Bogler DJ, Simpson BB. 1995 A chloroplast DNA study of the Agavaceae. Syst. Bot. 20. 191-205. 40 taxa. Agav:Dracaena, Sansevieria, Beaucarnea, Calibanus, Dasylirion, Nolina, Beschorneria, Agave, Manfreda, Polianthes, Prochnyanthes, Furcraea, Yucca, Hesperaloe. Hypox:Hypoxis. Lili:Asparagus, Aspidistra, Convallaria, Liriope, Maianthemum, Cordyline, Hemerocallis, Hosta. Xanthor:Xanthorrhoea. (PMBD, 186000297).
Ojeda Revah L, Ludlow Wiechers B. 1995 Palinologia de Agavaceae, una contribucion biosistematica. Bol. Soc. bot. Mex. 56. 25-43. LM, SEM. 15 gen., 36 spp. Agave (3 spp.), Beaucarnea (3), Beschorneria, Cordyline (2), Dasylirion (2), Dracaena (2), Furcraea (4), Hesperaloe (2), Hosta, Manfreda (5), Nolina (2), Phormium, Polianthes (2), Sansevieria (2), Yucca (5). (PMBD, 186000774).
Alvarez de Zayas A. 1996 El genero Furcraea (Agavaceae) en Cuba. (The genus Furcraea (Agavaceae) in Cuba.) An. Inst. Biol. Univ. Nac. Auton. Mex., Bot. 67. (2): 329-346 (1996) - illus. Icones, Maps, Anatomy and morphology, Keys. Furcraea antillana sp. nov. Geog=4 (KR, 199700692).
Souza Chies TT, Bittar G, Nadot S, Carter L, Besin E, Lejeune B. 1997 Phylogenetic analysis of Iridaceae with parsimony and distance methods using the plastid gene rps4. Pl. Syst. Evol. 204. 109-123. Alophia, Aristea, Babiana, Belamcanda, Bobartia, Crocosmia, Crocus, Cypella, Dietes, Freesia, Gladiolus, Iris, Isophysis, Lapeirousi a, Libertia, Micranthus, Moraea, Neomarica, Nivenia, Patersonia, Pillansia, Romulea, Sisyrinchium, Sparaxis, Tigridia, Trimezia, Wat sonia, Agav:Agave, Asparagus, Furcraea, Haemanthus, Hymenocallis, Narcissus, Nerine, Yucca. (PMBD, 186001815).
Khan HA. 1997 Morphotaxonomical studies of Furcraea (Agavaceae) of India. J. Pl. Anat. Morphol. 7. (2): 140-147 (1997) - illus. Icones, Keys. Geog=6 (KR, 199904195).
Garcia Mendoza A. 1999 Una especie nueva de Furcraea (Agavaceae) de Chiapas, Mexico. Novon 9. (1): 42-45 (1999) - illus. Icones, Anatomy and morphology. Furcraea niquivilensis sp. nov. Geog=4 (KR, 199900858).
Kauff F, Rudall PJ, Conran JG. 2000 Systematic root anatomy of Asparagales and other monocotyledons. Pl. Syst. Evol. 223. 139-154. LM. . Agap:Agapanthus; Agav:Agave, Furcraea, Hosta, Yucca; All:Allium, Tulbaghia; Amaryll:Amaryllis, Clivia, Hessea, Hippeastrum, Nerine; Antheric:Anthericum, Chlorophytum; Aphyll:Aphyllanthes; Asp:Asparagus; Behn:Behnia; Convall:Aspidistra, Beaucarnea, Convallaria, Dasylirion, Dracaena, Eriospermum, Nolina, Peliosanthes, Polygonatum, Reineckea, Sansevieria, Danae, Ruscus, Semele; Herreria, Hyac.:Albuca, Eucomis, Ledebouria, Muscari, Ornithogalum... (PMBD, 186100541).
Garcia Mendoza A. 2000 Revision taxonomica de las especies arborescentes de Furcraea (Agavaceae) en Mexico y Guatemala. Bol. Soc. Bot. Mex. no.66. 113-129 (2000) - illus. Icones, Maps, Chromosome numbers, Anatomy and morphology, Keys. Furcraea martinezii sp. nov. Geog=4 (KR, 200200674).
Nadaf AB, Shukla SR, Krishnan S. 2001 Some new observations in Furcraea foetida L. J. Econ. Taxon. Bot. 25. (2): 275-278 (2001) - illus. Icones, Anatomy and morphology. Geog=6 (KR, 200203359).
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It is usually assumed that the demographic transition (major intrinsic changes in fertility / mortality and life expectancy) is a purely human population phenomenon.  There are several explanatory theories that suggest the next causes: (1) social development [Condorcet, 1794], (2) economical/technological changes [Galor,O. 2011], (3) evolutionary change [Clark, G. 2007].
 However, these factors (in some degrees) operate within non-human populations. Moreover, some of the eco-evolutionary models indicate that the demographic transition may be a common consequence of co-selection adaptation of individual to each others within group) in hierarchically structured populations.
There are several (not all) cases in which demographic transition can be suspected:
  1. Regular extinction/ reemerging of local populations without apparent external reasons.
  2. Pronounced long-term demographic changes, which cannot be explained by environment variations or inbreeding depression. 
  3. Sudden appearance of unusually old or unusually big individuals in populations. 
  4. Sadden epidemic outbreaks of previously limited infections. 
  5. Visible absence of equilibrium size (carrying capacity); variations of population size weakly correlated with environment factors. 
  6. Fusion/split of local populations.
So, is a demographic transition in the non-human populations? I would be very grateful if you share your thoughts or maybe data that will help answer this question.
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I'm not convinced that a "simple, logical, biologically-based explanation" for DT is possible.  The social/political/economic behavior of humans is too complicated to be completely explained by biology.  The key feature in the DT is initial decline in death rate and then decline in birth rate.  Decline in DR occurs with better health care, hygiene and sanitation systems, improved nutrition and reliable food supply. Decline in BR occurs with greater willingness of females (and males) to use contraceptives. Use of contraceptives and family planning becomes more prevalent as women become better educated and take control of their own reproductive futures.  At a fundamental level, DT is the composite outcome of choices that humans make. Certainly, there may be some innate biology in action when a person makes a social and economic choice.  But this innate biology certainly does not explain DT.  It is tempting to want a biologically-based explanation for DT because if it is based in biology it might be seen as more scientific and valid.  However, it already is scientific and valid.  I agree that "the theory with greater scientific value will have a greater impact" .... but with regard to DT, "impact" on what?  We need DT to have an impact on politicians and policy-makers (particularly in the USA) in the form of awareness and realization that it is a good thing for a country and humanity in general. A speculative and unnecessary biological explanation will not help.
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Anuran tadpoles respond to chemical cues of predation reducing foraging and swimming activity. In many cases this behavior is produced by a predation event releasing different kind of cues, in particular alarm cues, coming from tadpole itself, seem to play a key role in elicit antipredatory responses, even if they often need to be associated to kairomone (from predator) to have the whole response.
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In terms of actual chemical characterization, none have been described (to my knowledge). However, Fraker et al 2009 (Horm Behav) showed a nice neuroendocrine response from a skin-released compound in larval amphibians. Fish have been well described (e.g., ostariophysan alarm pheromone). The Fraker paper has a nice summary of what is known as well. I doubt my answer helps, but I would also love to know if you have any updates on this question.
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With some colleagues from Simon Fraser University, we are developing a mathematical model to understand the evolution of male mating tactics in the framework of female fitness. It would be nice to know what observational/experimental studies (if any) have found.
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Thanks Ramakrishnan !!!
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NCBI Genbank hosts enormous numbers of COI sequences. However, it is not explicit how many species have their COI being sequenced, since there is also so many multiple sequences from one single species. I followed the keywords provided by Kwong et al (2012) to pull out the metazoan COI sequences (link provided below), with which I can obtain ~900,000 sequences, but cannot get the number of species information.
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"You will never be certain the obtained number is 100% correct "
agreed, thats why is wrote: "roughly"
- To retrieve sequences, I use my own programs (or via the eutils), not entrez which is not precise enough most of the time.
- then I filter on clade and minimal length.
- then I cluster sequences at some given % of similarity (needleman-wunsch, not blast).
knowing a number of manually confirmed sequences :
=>
- identify proper cluster(s), extract sequences & other infos from the genbank or embl entry.
- if new searches required in the future => try to estimate the best % of similarity to try to get every proper sequence but no non-target sequence in a single cluster (not always feasible).
Of course, this will need writing some lines in ... python of course
Best
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In the last decades, ecotoxicological studies have highlighted the toxicity of many different substances as well as the negative effects that variations in environmental parameter might have, as predicted by climate change scenarios, for example. Up till now, the majority part of works conduced in this field have used different kind of indicator organisms (mainly invertebrates), organisms exposed for a short (hours), medium (days/weeks) and long (weeks/months) time period. The 'problem' is that we have focused our attention, in most of the cases, in determining 'the effects' on a precise stage of the life cycle of a specific organism, driving on the conclusion that 'conditions will negatively affect this or that species'. But we all know that selection can occur. The study of some traits such as gonad maturation, quality of gametes and their interaction as well as embryos/larvae survival, growth and their performance at different experimental conditions might help us understand if a negative effect at the level of species can occur or if they have the potential to survive and adapt to a new and stressful condition across generations.
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Evolution is driven by the populations' ability to cope with environmental change. As such, pollution is a challenge as any other. I believe we can look at this under two different scopes: 1) a high toxicological challenge may reduce the number of individuals rather arbitrarily, diminishing genetic diversity and therefore the ability of a population to cope with change; 2) a low, albeit persistent, impact may drive the population to adapt to pollutants. This happens without human hand, of course, if one thinks about organisms living in highly toxic environments, such as deep-sea vents.
With respect to biomonitoring, it is pretty clear that wild organisms respond differently when compared to laboratory animals. Among other factors (of which toxicant-naïveness is paramount, although unrelated to evolution), it is most probable that species from impacted areas have adapted through directional natural selection. Researchers with both field and laboratory experience are well acquainted with the disparities between laboratory and wild animals - often yielding contradictory results if not contradicting ecotoxicological theory itself.
Linking population genetics to ecotoxicology is a brilliant, although not novel, idea. It has been attempted in the past but seems to have lost some focus, regrettably.
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I am interested in estimating what mechanisms support, or are promoting, high levels of sympatry between two species of bats. The two species are insectivorous, gregarious, very abundant, share the same shelter (caves), share a broad geographical distribution and are located in a variety of environments (low forest, high forest, desert scrub, agricultural crops, etc..) but always living together. Is a robust methods to quantify niche overlap the best way? Any suggestion? What would be the best approach to this problem?
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Hi Ricardo
Is possible that this paper might help you
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Suppose I have got a pair of sequences (100 amino acid each), one is conserved cds and another one is a non-conserved coding sequence.
1. Which one should be used for phylogeny?
2. Do these two types of sequences have different applications?
3. Second part of my query is whether DNA or amino acid sequence is more reliable for MSA and Phylogeny?
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Hi there,
You want something conserved enough that you can be assured you are working with orthologous sequences, but it also needs to have some freedom to change over time so that you can find patterns in historical differences in order to build your phylogeny. When trying to resolve deep phylogenies, more conserved sequences are likely more useful. But if you are trying to tease apart recent divergences, you may find quickly evolving regions to be very useful as long as you can still be sure of orthology.
UC Berkeley offers a graduate level course entitled Principles of Phylogenetics, and that course maintains a website where all of the readings, labs, and lecture notes are available for free. If you want to delve deeper, you should check it out. I think you will find the resources to be pretty interesting: http://ib.berkeley.edu/courses/ib200b/IB200B_SyllabusHandouts.shtml
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How the environment the prey has been caught in, and prey itself can affect snake’s cranial morphology? Which bones and their features (length, width, height) affect snakes adaptation (fitness)? I'm particularly interested in data on the core Macrostomata group (pythons and boas).
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Thank you Gordon W Schuett for your answer. I will check David Cundall work. Im already reading about intramaxillary joint in O. Reippel works but I will also check Cundall & Irish (1989) work.
Im the most interested in for example leght of quadrate and supratemporale (and other bones) on maximum size of prey consumed by snake. Which bones are usefull in what environment. More precisly what propotions of snake bones have influence on snake fittnes in particulary environment. I'm also looking for work about sexual dimorphism in Boidae and Pythonidae.
Some interesting works are already done for Natricinae between sexual dimorphism and prey catching environment.
Hibbitts, T.J., Fitzgerald, L.A., 2005. Morphological and ecological convergence in two natricine snakes. Biological Journal of the Linnean Society. 85, 363–371. doi:10.1111/j.1095-8312.2005.00493.x
Vincent, S.E., Moon, B.R., Shine, R., Herrel, A., 2006b. The functional meaning of “prey size” in water snakes (Nerodia fasciata, Colubridae). Oecologia 147, 204–211. doi:10.1007/s00442-005-0258-2,
Shine, R., 1986. Sexual differences in morphology and niche utilization in an aquatic snake, Acrochordus arafurae. Oecologia 69, 260–267.
Borczyk, B., 2014. Allometry of head size and shape dimorphism In Grass Snake (Natrix natrix). Turk. J. Zool. (In press).
Hampton, P.M., 2011. Comparison of cranial form and function in association with diet in natricine snakes. J. Morphol. 272, 1435–1443. doi:10.1002/jmor.10995 - And I'm looking for something similiar for other snakes.
I already read thoose works:
Arnold, S., 1993. Foraging theory and prey-size-predator-size relations in snakes. Ecological Behaviour, New York: McGraw Hill Seigel RA, Collins JT, 87–115.
Aubret, F., Bonnet, X., Harris, M., Maumelat, S., 2005. Sex Differences in Body Size and Ectoparasite Load in the Ball Python, (Python regius). J. Herpetol. 39, 315–320. doi:10.1670/111-02N
Bertona, M., Chiaraviglio, M., 2003. Reproductive biology, mating aggregations, and sexual dimorphism of the Argentine Boa Constrictor (Boa constrictor occidentalis). J. Herpetol. 37, 510–516.
Camilleri, C., Shine, R., 1990. Sexual Dimorphism and Dietary Divergence: Differences in trophic Morphology between Male and Female Snakes. Copeia 3, 649 – 658.
Chiaraviglio, M., Bertona, M., Sironi, M., Lucino, S., 2003. Intrapopulation variation in life history traits of Boa constrictor occidentalis in Argentina. Amphib. Reptil. 24, 65–74.
Feldman, A., Meiri, S., 2013. Length–mass allometry in snakes. Biol. J. Linn. Soc. 108, 161–172. doi:10.1111/j.1095-8312.2012.02001.x
Forsman, A., Shine, R., 1997. Rejection of non-adaptive hypotheses for intraspecific variation in trophic morphology in gape-limited predators. Biology Journal of the Linnean Society 62, 209–223.
Frazzetta, T.H., 1959. Studies on the morphology and function of the skull in the Boidae (Serpentes). Part 1. Cranial differences between Python sebae and Epicrates cenchris. Bulletin of the Museum of Comparative Zoology. 119, 453–472.
Frazzetta, T.H., 1975. Pattern and Instability in the Evolving Premaxilla of Boine Snakes. American Zoologist 15, 469–481. doi:10.1093/icb/15.2.469
Gans, C., 1961. The Feeding Mechanism of Snakes and Its Possible Evolution. American Zoologist 1, 217–227.
Greene, H.W., 1983. Dietary Correlates of the Origin and Radiation of Snakes. American Zoologist doi:http://dx.doi.org/10.1093/icb/23.2.431
Greene, H.W., Burghardt, G.M., 1978. Behavior and phylogeny: constriction in ancient and modern snakes. Science 200, 74–77. doi:10.1126/science.635575
Lee, M.S.Y., Bell Jr., G.L., Caldwell, M.W., 1999. The origin of snake feeding. Lett. Nat. 400.
Luiselli, L., Angelici, F.M., 1998. Sexual size dimorphism and natural history traits are correlated with intersexual dietary divergence in royal pythons (python regius) from the rainforests of southeastern Nigeria. Ital. J. Zool. 65, 183–185. doi:10.1080/11250009809386744
Monteiro, L.R., 1998. Ontogcnetic changes in the skull of Corallus caninus L., 1758 and Corallus enydris L., 1758 (Serpentes: Boidae), an allometric study. SNAKE-NITTAGUN- 28, 51–58.
Pearson, D., Shine, R., Williams, A., 2002. Geographic variation in sexual size dimorphism within a single snake species ( Morelia spilota , Pythonidae). Oecologia 131, 418–426. doi:10.1007/s00442-002-0917-5
Pough, F.H., Groves, J.D., 1983. Specializations of the Body Form and Food Habits of Snakes. American Zoologist 23, 443–454. doi:10.1093/icb/23.2.443
Rodríguez-Robles, J.A., Bell, C.J., Greene, H.W., 1999. Gape size and evolution of diet in snakes: feeding ecology of erycine boas. J. Zool. 248, 49–58. doi:10.1111/j.1469-7998.1999.tb01021.x
Shine, R., 1994. Sexual Size Dimorphism in Snakes Revisited. Copeia 1994, 326. doi:10.2307/1446982
Shine, R., Harlow, P.S., Keogh, J.S., Boeadi, 1998. The influence of sex and body size on food habits of a giant tropical snake, Python reticulatus. Funct. Ecol. 12, 248–258. doi:10.1046/j.1365-2435.1998.00179.x
That is what I have found but I'm still searching. If anyone have some suggestions or doing research about it, I will be very gratefull to hear about it. I'm looking for those informations for my Master degree paper.
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See above.
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I think many people would argue that genetic differentiation per se is not the best metric for determining whether two populations are incipient species, but rather that the amount of reproductive isolation (RI) should be used. Such RI should be genetically based to be persistent, but, to make an extreme example, it's possible that a very small genetic difference (i.e., one allele) could generate complete RI, while in another scenario loads of genetic differentiation may have no effect on RI. The answer is, as often, it depends. See reviews by Nosil & Schluter and Servedio et al. both 2011 in TREE.
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I will explore population structure within a species. But the structure did not work in my computer. Are there any other softwares which can be used and easily understand?
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The main Bayesian programs besides Structure are BAPS, Geneland, and TESS. Most journals that I have submitted to always recommend two programs to verify assignments. I typically run one set with non-spatial coordinates (Structure) and one with spatial coordinates (BAPS). Structure's non-spatial model is the best in my experience and the other three perform best with spatial information (both Geneland and TESS require spatial coordinates to perform best).
BAPS's spatial models are really great, but the non-spatial clustering of individuals or groups tends to give a high number of false clusters if you have strong isolation-by-distance. Geneland and TESS also suffer from this problem, but even more so.
So, for Structure, the MCMC runs are on the output, it just doesn't create a folder? I have Structure running on both my Mac and PCs (Win 7 and 8), and have not had that problem. It may be a problem with your computer instead of the program. I have experienced several bugs with Structure, but usually it fails to runs when I try to execute a job. That bug requires you to shut down structure and reload the job. Good luck, if you can only run a single Bayesian program, I would back it up with something like a PCA that shows concordant population structure.
Good luck.
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Journal articles regarding their hermaphroditism would be very helpful and any other useful information from reliable sources.
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Dear Carlos
Also take a look at the attached paper.
Best regards,
JP
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We are planning to place remote DVR cameras next to Broad-winged Hawk nests. We have a few questions from those that have already used these cameras on nests: How much memory was used per hour of footage (at 720)? How often did memory have to be downloaded from cameras? How did changing batteries and downloading data from the cameras impact the nesting birds? When did you put cameras next to the nest? When they were on eggs? Before they were on eggs? When young hatched? Thanks to all that respond.
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Awesome! Thanks Ronny.
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For example, in the co-evolution of plants and insects, birds or any other organism that pollinates it, the mutations they acquire with time are merely by chance or are there some signals which lead to positive mutation whereby it depends on the interaction with environment for better survival?
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There are known mechanisms of biased mutation rates, spots, regulation, etc. that are results of past selection on the plasticity and evolvability of organisms and populations. There is also physico-chemichal bias on mutation. But the outcomes continue to happen probabilistically and the effects on fitness depend of a new round of selection to produce adaptation.
Some confusion arise because two class of errors:
1 – Conflation of mutation being random in relation to fitness and random as a molecular event.
2 - Confusion between randomness with equiprobability. Mutations don’t have to be equiprobable to remain random in origin. It's enough that they have any probabilistic distribution - i.e. are not detereministic.
There are evidence of coevolutionary scenarios between parasites-hosts where mechanisms of biased mutation/mutability are known on bacteria, mainly for traits related to bacterial resistance and responses to stress. This is less likely in multicellular organisms, mainly on those with a rigid division of soma and germ-line tissues.
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I'm planning to do a population genetic analyses (genetic differentiation, diversity) on a bee species using SNP markers. Furthermore, I aim to identify genes under selection. For population genetic studies based on microsatellites, ususally it is recommended to sample at least 30 individuals per population to have enough power. Does this recommendation also hold for SNPs, since they are biallelic and thus less variable than microsatellites (but also more abundant)? What is your opinion about this? How many samples do I need for the population genetic part and how many for the study of adaptation?
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Recently I came across a similar question for a fish species. Remember browsing through a few articles. Some information that I could quickly browse again for you:
"Given that each SNP marker has an individual evolutionary history, we calculated that the most complete and unbiased representation of the genetic diversity present in the individual can be obtained by incorporating at least 10 individuals into the discovery sample set, to ensure the discovery of both common and rare polymorphisms" [PMID: 21207104 - a study on primates]
Other potentially important articles (pubmed IDs):
22685040
18288444
20555335
17227482
16258542
16137993
15812170
11836209
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Something intrigues me about how many unrelated species of birds happily eat the hottest of hot peppers. It leads me to think it might be an ancient adaptation. But, capsaicin is restricted to Capsicum whose origin is the Neotropics. That should rule out a very early link. So how do so many non-neotropical species eat them so avidly - like the domestic chicken which is originally Indian?
Have innumerable species of birds independently evolved to eat capsaicin and, if so, why haven’t mammals taken the same path? The fruits are bright red clearly to attract birds, but they are also very aromatic which mammals would easily notice.
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Just a few clarifications on this.
1. Birds DO have the TRPV1 receptor like mammals, which is also commonly called the capsaicin receptor. However the bird TRPV1 is insensitive to capsaicin as beautifully shown by the work in David Julius lab (Jordt S-E and Julius D (2002) Molecular basis for species-specifig sensitivity to "hot" chili peppers. Cell 108: 421-430).
2. Some of the suggestions included by previous comments have already been tested experimentally by Josh Tewksbury (Tewksbury JJ and Nabhan GP (2001) Directed deterrence by capsaicin in chillies. Nature 412: 403-404 and Tewksbury JJ, Reagan KM, Machnicki NJ, Carlo TA, Haak DC, et al. (2008) Evolutionary ecology of pungency in wild chilies. ProcNatAcadSciUSA 105: 11808-11811).
The first paper proves that chilli seed germination is decreased in the GI tract of mammals but not by the passage through the GI tract of birds. In this case birds can benefit from the rich contents of the chilli peppers by not being sensitive to the capsaicin. The plant benefits by getting the seeds dispersed efficiently.
The second paper has little to do with birds but it is a demonstration that an increase in the amount of capsaicinoids in fruits reduces fungal infection and seed mortality.
Obviously these papers do not provide all the answers so there is plenty to be studied in this respect. In the past I tried to collect evidence of which bird species are insensitive to capsaicin but have found very little. The fact that capsaicin does not activate the chicken TRPV1 could mean that this is a general feature for all birds because chickens are rather basal birds. It was interesting to read that hummingbirds avoid chillis but many bird species are neophobic to new tastes and that does not necessarily mean that they do sense the heat in the chillis.
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I have been studying the numerous similiarities that the catfish and sharks have in common. I have studied the writings of Dr. D. Whitehead of Australia as he compared the Ampullae of Lorenzini of both types. I am wondering if any other researcher has taken up the tasks of comparing the catfish to the sharks or vice versa. I would be very interested in the testosterone levels especially of bullheads and the bull sharks.
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Dr. Kovalchuk:
Thank you so very much for the books. I will read them earnestly. They will definitely be helpful. I do appreciate your efforts. Do you know of any research that compares characters of sharks to the same type of characters of catfishes. I am in the process of studying Dr. Darryl Whitehead's work on the comparison of the catfishes and shark's ampullae of Lorenzini.
Please keep in touch and keep those references coming that you think will help me in my quest.
Thanks again:
James Burgess
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I am trying to determine the genetic differentiation between various groups of populations based on Nei’s genetic distance. For the fixation index Fst Wright (1978) has suggested qualitative guidelines for interpretation:
From 0 to 0.05 may be considered as indicating little genetic differentiation;
From 0.05 to 0.15 indicates moderate genetic differentiation;
From 0.15 to 0.25 indicates very great genetic differentiation;
Above 0.25 indicates very great genetic differentiation.
Is there a similar guideline for Nei’s genetic distance?
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Also the suggested ranges for Wright 's fixation index are more a rule of thumb and you should not rely on them too much, since they are for biallelic markers. For highly polymorphic and multiallelic markers, values will be much lower which might lead to a completely wrong interpretation.
Because of this I'd avoid such generalising guidelines, but do a comparison with data from other works that used the same or different species with perhaps similar biology (e.g. in terms of their biology, dispersal, mating system, overwintering, etc) or in a similar situation (e.g. if you look for possible dispersal barriers).
And finally a good approach for genetic distance is often doing an Isolation by distance analysis, as it gives you an idea about the correlation between genetic and geographic distance (which you can also do statistic tests if you want).
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Fitness is the product of interaction of a trait/gene/adaptation with a selection process. So all the population studies that claim to measure Selection pressure by measuring the changes in the frequency of a particular trait/gene/adaptation are in fact measuring the fitness of trait/gene/adaptation against the selection, while the selection process is itself not quantified. This makes me wonder if Selection is a quantifiable feature. I want to distinguish selection and fitness as the challenge and the response. While it is okay to say Positive and negative selection, is there a weak selection vs strong selection? or is it rather that we see poor fitness vs good fitness of an trait/gene/adaptation? For ex. Tardigrades are known to survive in extreme conditions that no other organism can survive. While these conditions might be selection factor for other organisms, Tardigrades may not respond to these changes. If one were to use the population changes of tardigrades under ionizing raditation to measure the selection pressure, it may lead to the notion that ionizing radiation is not a strong selection pressure. But, if one were to test it on human cells, it might select favorably a population of highly radiation resistant cells. I am not sure if selection varies with target, or does fitness of the target varies with selection? If the latter is true, then aren't Selection coefficient and other terms used to describe fitness as "selection-" are a misnomer? Based on the above example, for the same selection, we get different response and hence fitness changes not selection.
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Daniel,
I am not saying that you cannot differentiate between two selections based on their effect on identical population. I just posing the problem that the comparing these two selection condition could tell differently about their relative strength for if tested on different set of identical populations. Which makes it difficult to predict what would happen if the same selection is applied to a third population. If you kept selection constant (non-quantitative), then we can interpret population changes solely on fitness. I agree that you need both the parts, one of which is not practical (at least because we cannot measure the impact of that selection on all the various variations). If fitness dictates selection, there is no additional information in knowing selection's numerical value other than the fact that there was selection. You can explain change in population and predict future changes on population solely on Fitness. Either one of the models will not work as long as you want to quantitate both. As the current models try to quantitate and compare different selections, they compare their effect only on one population. I think the problem will become apparent only when two different groups study the same selection condition that say that "in a population, A type selection is stronger than B" and the other group says that it is vice-versa. I guess until I find two studies that are already out there or such a study comes up the problem with the interpretation of selection from fitness will not apparent. Anyway- Thanks a lot for the discussion.
Best,
Sathiya
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The cost-benefit model for the evolution of carnivory in plants, proposed by Thomas Givnish (1984), was experimentally tested only a few times and the results are quite contradictory. Certain studies show the results expected according the model (Farnsworth & Ellison, 2008; Pavlovic, 2009), while others show the contrary (Mendéz and Karlsson, 1999; Wakefield et al. 200; Adamec, 2008). In spite of this ambiguity, Givnish’s theory is still considered to be true, at least by most authors. Is this the result of some kind of inertia in the area or because of the lack of a better explanation?
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The cost/benefit model proposed by Thomas Givnish is, with slight modifications, probably true and as far as I know, nothing can better explain evolution of botanical carnivory. of course, each experiment you perform is dependent on dose of nutrients and time needed for growth of new leaves. For example, mendez and karlsson measured photosynthesis only 1 week after feeding. Wakefield did not measure photosynthesis on new leaves but only on them that were fed. It seems that for aquatic carnivorous plants, carnivory stimulate growth of shoot apex and Ellison and Adamec (2011) modified cost/benefit model for aquatic carnivorous plants. Or see also Lubomír Adamec, 2011. By which mechanism does prey capture enhance plant growth in aquatic carnivorous plants: Stimulation of shoot apex?, Fundam. Appl. Limnol. Vol. 178/2, 171–176. Also the respiratory cost must be taken into account at least in plant with active trapping mechanism (e.g. see my publication in Ann Bot., Pavlovič et al., 2010 or Laakkonen modification in Utricularia: A new model for the evolution of carnivory in the bladderwort plant (utricularia): adaptive changes in cytochrome C oxidase (COX) provide respiratory power. Plant Biol (Stuttg). 2006 Nov;8(6):758-64.
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Humans appear to be, by and large, one of the exceptions to this general rule.
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Our lab group is starting to get interested in this subject. To date, probably the best reviews are by Mark Kristal (1980. Neuroscience and Biobehavioral Reviews 4:141-150. and 2012. Ecology of Food Nutrition. 51:177-197). Most recent papers suggest only humans, cetaceans, and cammelidae (actually I've only found references to Llama) do not eat the placenta. However, this appears to be much more variable than has been reported (e.g., domesticated goats occasionally eat some of the placenta but this varies from 10 to 40% of births meaning the majority of mothers do not). Really the only good data are for lab rats and lab mice where placentophagy appears to occur in all births with mothers eaten the entire placenta.
The idea that this is a 'high quality food' is commonly suggested but there are real problems with that. The main one is that virgins of both sexes seem to be literally repulsed by it. Second, new mothers are often very eager to eat the placenta but as Alan mentioned, may avoid other organ meat. In fact, this attraction appears to be short term. Finally, it seems odd that many herbivores should suddenly desire to eat a placenta.
Removing a potential predator attractant makes some sense to me but obviously that might not apply to all cases as James said. Immediate removal of the placenta might reduce disease risk as I've seen some evidence cows, especially the newborn, suffer higher fungal infections when the placenta is not removed (unfortunately I've lost that reference).
There is mixed evidence that mother's who eat the placenta produce more milk than those prevented from doing so. There is also some evidence that the content of the milk changes. However, these are all very old studies (mostly pre-WW II).
Kristal's main hypothesis is that the placenta acts as an analgesic although no one knows what chemical is doing this. He presents some good evidence for this effect in rodents. If this were generally true, I would expect placentophagy to be more common in species that have difficult births (like humans) or multiple births. The goats mentioned above are more likely to eat the placenta if they have twins than with single births. I've been looking for more data to back this up but haven't found much.
Basically, what we have found is that this is a very understudied problem.
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I saw it frequently in Hormuz Island in the Persian Gulf that Pina bivalve lives besides Carpet anemone. I attached one of my picture.
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As far as I recall, there is nothing like this in the literature. I am familiar with Pinna nobilis in the Mediterranean, and think there still is a lot to be learned about hte ecological relationshps of these bivalves. In your case, both bivalve and anemone benefit from small-scale turbulence in water flow. In your picture one can see some seagrass, so this habitats must be intertidal, possibly subject to currents. As a first step, I suggest you count how many times you see Pinna alone, anemone alone, and both together. If possible, map the occurrence of Pinna and anemone in a grid area large enough to have about 30 specimens of each species, along with habitat variables sucha as depth, seagrass cover, type of substrate… This is very interesting indeed!
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In this paper:
The delayed rise of present-day mammals.
Bininda-Emonds OR, Cardillo M, Jones KE, MacPhee RD, Beck RM, Grenyer R, Price SA, Vos RA, Gittleman JL, Purvis A.
Nature. 2007 Mar 29;446(7135):507-12.
PMID: 17392779
There is a beautiful tree of mammalian evolution, with monotremes and marsupials as "outgroup".
The tree figure seems to indicate that there were just 3 lineages that survived from a common ancestor some 160 million years ago, until mammals, marsupials and monotremes each began to diverge into present-day species some 80 million years ago.
Does this seem correct? How solid are these dates?
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The Lartillot data is available in NEXUS format in the data DRYAD:
Lartillot N, Delsuc F (2012) Data from: Joint reconstruction of divergence times and life-history evolution in placental mammals using a phylogenetic covariance model. Dryad Digital Repository. doi:10.5061/dryad.tt28qk6f
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I had previously asked a question "Can bacteria mediate "mating" in insects?" for which i had received wonderful answers from my peers. Taking all answers and reference into consideration we performed certain experiments to ascertain our hypothesis. We cultured a germ-free line (GFL) of B. dorsalis females. Virgin males were given a dual-choice between GFL and Non-GFL B. dorsalis females in a customized olfactometer. We were astonished as males were significantly (P < 0.0001) attracted to non-GFL B. dorsalis females.
We had previously identified 9 bacteria from the reproductive organ of the females and each bacteria was checked for their attractiveness. Two out of nine were attractive to virgin males. Is there a possibility that these bacteria produce "sex pheromones" that attract male towards female flies? Further work is under-process.
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Hello,
It is becoming more and more clear that the microbiota plays an astonishing role in animal and insects in particular. Usually, pheromones are produced by specific secretory cells. However, it would not be impossible that a primer released from such glands in a pouch could be finally transformed by microorganisms living in the pouch into the actual pheromone. An interesting side-question would be an the mechanism of inheritance of these micro-organims.
Best of luck with your experiments !
JLH
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I want to start a new project about swimming crab demographic evolution, and some specialists suggested some modern ways to get that information, and I read about SNPs and sound like a very good way to get this out. But I don't know how much it will cost if I have access (or... maybe) to the system EP1 of Fluidigm.
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You should first have some info:
-Ploidy level
-genome size
Then you should know:
-How accurate you have to be
-What is the question you need to answer
-Do you have to separate maternal/paternal origin…
It may influence the price …
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Im doing an investigation to determine the ideal parameters for cockles (in particular the common cockle). Chemistry levels of the water and sand quality for when they are not in water, and when they burrow in the sand while submerged would be ideal.
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The literature on this species is quite abundant:
or :
Malham, S.K., Hutchinson, T.H., Longshaw, M., 2012. A review of the biology of European cockles (Cerastoderma spp.). Journal of the Marine Biological Association of the United Kingdom 1, 1-15.
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We recently isolated 9 cultivable bacteria from the reproductive organs of B. dorsalis. Using 4-armed olfactometer, the isolated bacteria were provided to B. dorsalis as odor source. Out of the 9 bacteria, 1 bacteria attracted males, significantly (P < 0.0001). Is there a possibility of a bacterium acting as a mediator of "Sex" in insects. Can it be a symbiotic host-microbe interaction?
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There you go!
/m
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Woody species of Rhododendron all showed upper range contraction but upward shift in the center of maximum abundance/optimum temperature for R. arboreum but downward shift for R. barbatum and R. campanalutum.
What may cause both upward and downward shift in temperature optima / center of maximum abundance of different species of same genus along mountain gradient?
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Sometimes the mist induced by the orographic lift of air masses can create a visible vegetation belt on altitudinal gradients.
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In my understanding, negative frequency-dependent selection reduces in some measure the gene flow between different polymorphisms, whereas introgressive hybridization does the opposite. I'm also finding studies that defend frequency dependent selection and others that defend hybridization on haplochromine cichlids.
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Frequency-dependent selection has not to do with gene flow. In evolution you have 4 "forces" mutation migration selection drift. Gene flow is part of migration processes, while frequency dependent selection is part of selection processes.
From what you say, my guess is that what people want to explain on haplochromine cichlids is how polymorphism is maintained.
Gene flow (which translates into introgressive hybridization) is one potential explanation, because classical selection occurs at a place, and reduces the number of phenotypes. But gene flow brings new phenotypes from outside the place, which maintains polymorphism.
Negative frequency-dependent selection is an alternative explanation, because it is a type of selection that maintains polymorphism: with this type of selection, phenotypes are selected because they are rare, so they never become rare enough to disapear, and polymorphism is maintained.
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This term is used in the Global Environmental Stratification.
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Stratification is the layering of habitats within an ecosystem with the presence of distinctive physical and biological boundaries between the strata (layers). For example, in a temperate deciduous forest, you typically have 5 vertical layers: the canopy layer, the sapling layer, the shrub layer, the herbaceous layer, and the moss layer. Similarly, the stratification of a lake typically has three distinct layers: the Epilimnion (top layer), the Metalimnion (middle layer; may change depth during the day), and the Hypolimnion (the bottom layer). As such, ecosystem stratification is dependent on the ecosystem (e.g., the stratification of a forest is different than the stratification of a lake). Furthermore, ecosystem stratification does not only occur vertically, but can occur horizontally within an ecosystem as well.
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Speciation, "the origin of reproductive barriers among populations that permit
the maintenance of genetic and phenotypic distinctiveness of these populations in geographical proximity" (Seehausen et al 2014), is driven by a great variety of mechanisms as sexual selection, morphological adaptation, or hybrid advantage. I would like to know which mechanisms have been studied until now.
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There are some studies talking about hybridization between an introduced cichlid and the native species. Also some cases of hybridization are explained due to pollution events
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I am looking for any papers that already tried to link latitude and the timing of dawn song in common songbird. It seems it has never been investigated. Papers studying latitudinal clines regarding activity onset and offset in songbirds are also welcome.
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Just to stimulate discussion.
I have the impression that in Southern France birds sing more during rainy spring days.
If so, perhaps one explanation is that birds require minimum levels of humidity/rain to sing properly (cf. human song performers or those that present an oral presentation have to drink to continue singing/speaking). Perhaps dawn song is also favoured because of higher levels of humidity at dawn. Perhaps there might be a significant proximate interaction between latitude and time of the day (dawn versus later) /year (more song during the day in years with more humidity) influencing avian singing activity.