ELISA - Science method

Acute kidney injury (AKI) is among most dangerous and common complications after oper-heart cardiosurgical operations. Therefore, a search is carried out for biological markers which could timely detect this condition. The article presents dynamics of TGF-1, TGF-2, TGF-3 in blood serum prior and after a coronary artery bypass (CAB). The study inclu...

Purpose Helicobacter pylori infection is a well-established etiological factor for gastric inflammation and a significant risk factor for the development of gastric cancer. However, the precise relationship between dietary zinc intake and seropositivity for Helicobacter pylori remains uncertain. Methods This cross-sectional observational study uti...

Regular checkups for thyroid-stimulating hormone (TSH) levels are essential for the diagnosis of thyroid disease. The enzyme-linked immunosorbent assay (ELISA) technique is a standard method for detecting TSH in the serum or plasma of hospitalized patients. A recently developed next-generation ELISA, the digital immunoassay (d-IA), has facilitated...

COVID-19 vaccines were developed at an unprecedented speed in history. The factors affecting the response to COVID-19 vaccines are not clear. Herein, the effects of vitamin D and vitamin A (retinol) levels on the response to the BNT162b2 vaccine were explored. A total of 124 vaccine recipients were recruited from the general population attending va...

Background: To fight the COVID-19 pandemic, immunity against SARS-CoV-2 should be achieved not only through natural infection but also by vaccination. The effect of COVID-19 vaccination on previously infected persons is debatable. Methods: A prospective cohort was undergone to collect sera from unvaccinated survivors and vaccinated persons—with and...

Background: With the impact of SARS-CoV-2 upon public health directly and socioeconomically, further information was required to inform policy decisions designed to limit virus spread. This study sought to contribute to serosurveillance work within Northern Ireland to track SARS-CoV-2 progression and guide health strategy. Methods: Sera/plasma samp...

El diseño factorial completo de experimentos se ha utilizado con poca frecuencia para optimizar inmunoensayos, a pesar de sus ventajas en el ahorro de tiempo y recursos y sus resultados confiables. El objetivo de este trabajo fue optimizar un ELISA, que detecta anticuerpos IgA anti-RBD del SARS-CoV-2, mediante la aplicación de experimentos factoria...

The aim of this research was to develop a quantitative sandwich-strip ELISA was developed on a nitrocellulose membrane with anti-H alpha and anti-hCG beta antibodies. The detection antibody was conjugated to horseradish peroxidase, and a quantitative sandwich-strip ELISA with luminol substrate was developed following optimization with hCG. Concentr...

**Key Conclusions from the Webpage:** - The study aimed to determine the association between dengue virus (DENV) serotypes and dengue hemorrhagic fever (DHF) in DHF patients from Peshawar district, Pakistan . - All 200 blood samples collected from dengue fever (DF) and DHF patients tested positive for NS1 ELISA . - The study focused on the four ser...

Background Inflammasomes are a group of molecules that are strongly involved in causing inflammation. This study aimed to evaluate the expression of NLR family pyrin domain containing 1 (NLRP1), NLRP3, and Apoptosis-associated speck-like protein containing a CARD (ASC) as well as their association with serum level of interleukin (IL)-1β in patients...

West Nile virus (WNV) is a global important mosquito-borne Flavivirus causing West Nile disease (WND). In Libya, evidence of WNV circulation has been reported in humans but never in animals. The aim of this study was to determine the seroprevalence of the WNV infection in horses and dogs in Libya. A total of 574 and 63 serum samples from horses and...

Brassica napus L., winter oilseed rape (OSR), is a major crop worldwide, with a wide range of uses and high profitability. Viruses, such as turnip yellows virus (TuYV), are becoming increasingly important, and in certain years, they can cause significant infestations in OSR. It is difficult to detect the presence of the virus during visual field in...

Currently, biological markers for COVID-19 disease severity still constitute the main goal of enhancing an efficient treatment to reduce critical consequences such as an abnormal systemic inflammatory response. In this regard, the latest research has shown that Pentraxin 3 (PTX3), a highly conserved innate immunity protein, may serve as a valuable...

Abstract Depression is a common psychiatric disorder with high prevalence and mortality rates as well as high risk of serious harm in adolescents that have significant negative impact on families and society. The feeding inhibitor Nesfatin-1 contributes to the regulation of stress and emotion. The purpose of this project was to compare the differen...

To investigate the effects of low-level laser therapy (LLLT) as an adjunct to non-surgical periodontal treatment (NSPT) on the plasminogen-activating system. Stage 3–4 Grade C periodontitis and age-gender-matched healthy individuals participated in the split-mouth study (ClinicalTrials.gov identifier, NCT05233501). The study groups were Periodontit...

4 ، ‫سمستبن‬ 9313 79 ‫انگشت‬ ‫پنج‬ ‫گیاه‬ ‫برگی‬ ‫عصاره‬ ‫الکلی‬ ‫هیدرو‬ ‫اثر‬ (Vitex agnus-castus) ‫تستوسترون‬ ‫میزان‬ ‫بر‬ ‫نر‬ ‫صحرایی‬ ‫موش‬ ‫سرمی‬  ‫بهره‬ ‫مصطفی‬ ‫بر‬ * : ‫فیشیولوصی‬ ‫دهدشت‬ ‫دهدشت،‬ ‫واحد‬ ‫اسالمی،‬ ‫آساد‬ ‫دانشگبه‬ ‫جبنوری،‬  ‫هدایت‬ ‫یاورمقدم‬ : ‫شنبسی‬ ‫سیست‬ ‫دریب‬ ‫جبنوران‬ ‫دهدشت‬ ‫دهدشت،‬ ‫واحد‬ ‫اسالمی،‬ ‫آساد‬ ‫دا...

Background: The relationship between dietary carbohydrate intake and serum Klotho levels, an aging biomarker, remains uncertain. Objective: This study aimed to investigate the association between dietary carbohydrate intake and serum Klotho levels among American adults aged 40–79. Methods: We analyzed data from 10,722 adults aged 40–79 years who pa...

Background Food products with < 20 mg/kg gluten can be labeled ‘gluten-free’ according to international regulations. Several antibodies based ELISAs have been develop to track gluten traces in food products. Among them, R5 and G12 antibody-based ELISAs are the frequently used methods. However, these antibodies have certain limitations. Aims We eva...

The aim of the study is to investigate oxidative stress in sheep infected with hydatid cyst by determining the activities of Total antioxidants (TAOS) and Malonaldehyde (MDA) and their relationship in liver tissues. Fifteen infected livers and 15 healthy livers were included in the present study. The levels of TAOS and MDA in homogenized liver tiss...

Giriş: Yaşa bağlı maküler dejenerasyonu gelişmiş toplumlarda ileri derecede görme kaybının en sık nedenidir. ANXA3, hücre sinyal yolakları, hücre profilerasyonu, invazyon, metastaz ve hücre apoptozuna etki ederek tümör gelişimine katkı sağlamaktadır. Yöntemler: Bu çalışma yaş tip YBMD tanısı alan 39 hasta, aynı zaman diliminde kuru tip YBMD tanısı...

Embolization is the preferred method for treating intracranial aneurysms due to its less invasive nature. However, recent findings suggest that even uncomplicated embolization may cause structural damage to the brain through ischemic or inflammatory mechanisms. This study aimed to find possible biomarkers of brain injury and inflammation in patient...

Background: Vaccination is one of the most effective life-saving medical interventions, and the introduction of SARS-CoV-2 vaccines was intended to prevent the serious implications of COVID-19. The objectives of the study were: i) to observe the humoral immune response to BNT162b2 vaccine and SARS-CoV-2 infection (mainly breakthrough infections), i...

ACE2’s impact on the severity of COVID-19 is widely discussed but still controversial. To estimate its role in aspects of the main risk factors and comorbidities, we involved post-COVID-19 patients in Ternopil region (Ukraine). The recruitment period was from July 2020 to December 2021. Medical records, treatment modalities, and outcomes were recor...

Objective: The aim of this study is to investigate the relationship between leptin, nesfatin 1 and NPY hormones and zinc in boys and girls diagnosed with metabolic syndrome and obesity. Materials and Methods: This study included a total of 6 groups. Group 1 Boy Control, Group 2 Girl Control, Group 3 Obese Boys, Group 4 Obese Girls, Group 5 Boys wit...

Dengue fever, a mosquito-borne disease in tropical and subtropical climates caused by the dengue virus (DENV), has become a major social and economic burden in recent years. However, current primary detection methods are inadequate for early diagnosis of DENV because they are either time-consuming, expensive, or require training. Non-structural pro...

Introduction. Psychopathic traits have been widely found to be predictors of conduct problems. Parental practices have also been studied as mediators of these relationships. However, given the higher prevalence of these traits in boys, little is known about the specificities of these relationships in girls. Objectives. The present study aims to exa...

Osteoporosis, characterized by low bone mineral density (BMD) and increased fracture risk, is a prevalent condition. Individuals with type 2 diabetes mellitus (T2DM) are at a higher risk of developing osteoporosis, necessitating the identification of biomarkers for early detection and management. This study aimed to investigate the potential of adi...

Neutrophil gelatinase-associated lipocalin (NGAL) may promote development of inflammation in psoriasis, whereas proprotein convertase subtilisin/kexin type 9 (PCSK9) may account for dyslipidemia in some psoriatic patients. The aim of the study was to analyze the influence of cyclosporine therapy on serum levels of NGAL and PCSK9 in patients with ps...

Background The aim of our study was to evaluate the expression of the CASP3 gene at both mRNA and protein levels in patients with depressive disorders and to determine the impact of caspase 3 in the pathogenesis of depression; Methods A total of 290 subjects, including 190 depressed patients and 100 healthy controls, participated in the study. Soc...

Real-time quaking-induced conversion (RT-QuIC) assays have become common in the detection of chronic wasting disease (CWD) and are very sensitive provided the assay duration is sufficient. However, a prolonged assay duration may lead to non-specific signal amplification. The wide range of pre-defined assay durations in current RT-QuIC applications...

Objectives Diabetic retinopathy (DRP) is one of the most common microvascular complications of diabetes. The pigment epithelium-derived factor (PEDF) is a protein that is one of the most potent angiogenesis inhibitors. The effect of blood PEDF concentration on DRP formation remains unclear. The present study aimed to determine whether the plasma co...

Alkaline phosphatase (ALP) is a phosphomonoester hydrolase and serves as a biomarker in various diseases. However, current detection methods for ALP rely on bulky instruments, extended time, and complex operations, which are particularly challenging in resource-limited regions. Herein, we synthesized a MOF-derived Fe-N-C nanozyme to create biosenso...

Background Evaluating the effectiveness of Chagas disease treatment poses challenges due to the lack of biomarkers for disease progression and therapeutic response. In this study, we aimed to assess the clearance of Trypanosoma cruzi ( T. cruzi) parasites in a group of benznidazole (BNZ)-treated chronic Chagas disease patients using high-sensitivit...

Background: The aim of the study was to longitudinally investigate the serum levels of mesothelin, sestrin1, hyaluronan synthase 2 (HAS2), midkine, and high mobility group box 1 (HMGB1) before and after chemotherapy and at the time of relapse in malignant pleural mesothelioma (MPM) patients treated with chemotherapy and to compare the changes in b...

Electronic cigarettes have become increasingly popular, but the results of previous studies on electronic cigarette exposure in animals have been equivocal. This study aimed to evaluate the effects of electronic cigarette smoke (ECS) and cigarette smoke (CS) on lung function and pulmonary inflammation in mice to investigate whether electronic cigar...

Critical patients with pandemic COVID-19 have dysregulation in immune response, like cytokine storm, lymphopenia that led to increase mortality, vaccine is the only hope for controlling on this pandemic. However, this study design to evaluate effectiveness of Pfizer vaccine on serum level for IL-12/IFN-γ pathway with susceptibility to COVID-19. The...

Background and Objectives: Because of the controversial aspects of the CMV virus during pregnancy, it should be considered a serious health threat, especially in developing countries. The present seromolecular study aimed to determine cytomegalovirus prevalence in pregnant women referred to health centers in the north of Iran. Materials and Methods...

Background Although handgrip strength (HGS) asymmetry has clinical screening utility, its relevance to sarcopenia is unknown. This study examined the relationship between HGS asymmetry and sarcopenia signatures, and explored the relevance of circulating neural/neuromuscular markers. Methods 9403 individuals aged 18–92 years participated in this st...

The current study was conducted in Maysan province, Southern Iraq. Blood samples were collected from 174 individuals (male and female), from December 2020 to May 2021. ELISA was used to calculate the seroprevalence of Toxoplasma gondii by determining the levels of anti-toxoplasma IgM and IgG, as well as the levels of dopamine and adrenaline in the...

Collagen-binding proteins (CBP), Cnm and Cbm, from Streptococcus mutans are involved in infective endocarditis caused by S. mutans because of their collagen-binding ability. In this study, we focused on the collagen-binding domain (CBD), which is responsible for the collagen-binding ability of CBP, and analyzed its structure, binding activity, and...

The aim of this study was to investigate how zinc deficiency and supplementation affect liver markers including autotaxin, kallistatin, endocan, and zinc carrier proteins ZIP14 and ZnT9 in rats exposed to maternal zinc deficiency. Additionally, the study aimed to assess liver tissue damage through histological examination. A total of forty male pup...

The recombinant antibodies RB792, RB793, RB794, RB795, RB796 and RB797 recognize by ELISA a synthetic phosphorylated peptide from the human NDC80 protein.

The recombinant antibodies RB798, RB799, RB800, RB801, RB802, RB803, RB804, RB805, RB806, and RB807 were tested by ELISA for their binding to a synthetic phosphorylated peptide from the human Aurora kinase B (AurKB) protein. Antibodies RB805 and RB806 detected both the phosphorylated and non-phosphorylated peptides, whereas the remaining antibodies...

Objective There is an urgent need for novel biomarkers in lupus nephritis (LN). We report a non-invasive urinary biomarker, L-selectin, in two independent multi-ethnic cohorts. Methods uL-selectin was tested cross-sectionally in a Chinese cohort (n=255) and a US cohort (n=219) of SLE patients and controls using ELISA. A longitudinal cohort include...

Oxytocin (OT), a neuropeptide known for its role in social bonding, trust, and emotional regulation, has gained significant attention in recent years. Various studies have explored the effects of different environmental stimuli on the secretion of OT, with external frequencies being one such stimulus of interest. This study aimed to investigate the...

Abstract Background Despite the high sensitivity of cerebrospinal fluid (CSF) amyloid beta (Aβ)42 to detect amyloid pathology, the Aβ42/Aβ40 ratio (amyR) better estimates amyloid load, with higher specificity for Alzheimer’s disease (AD). However, whether Aβ42 and amyR have different meanings and whether Aβ40 represents more than an Aβ42-corrective...

Introdução: A Doença de Chagas (DC), causada pelo protozoário Trypanosoma Cruzi, é considerada uma das 20 principais doenças tropicais negligenciadas e que ainda aflige muitos países da América Latina, incluindo o Brasil. Os principais mecanismos de transmissão vetorial são a ingestão de alimentos contaminados e a transmissão vertical. Objetivo: In...

Minimizing error in prevalence estimates and diagnostic classifiers remains a challenging task in serology. In theory, these problems can be reduced to modeling class-conditional probability densities (PDFs) of measurement outcomes, which control all downstream analyses. However, this task quickly succumbs to the curse of dimensionality, even for a...

Purpose: To investigate the role of IL-36 in the tumorigenesis of hepatocellular carcinoma (HCC). IL-36 composed of a natural antagonist (IL-36Ra) and three agonists (IL-36α, -β, -γ) that stimulate inflammation by binding to a common receptor consisting of IL-36R and IL-1RAcP. HCC is a common malignancy associated with high morbidity and mortality...

Objective The incidence of hyperlipidemic acute pancreatitis (HLAP) has rapidly increased in recent years in China. Autophagy has been implicated in the inflammatory response of pancreatic cells in HLAP, but the molecular mechanisms remain unclear. Methods In this study, the role of HIF-1α–PPARγ–mTORC1 pathway-mediated autophagy in the inflammator...

The present study was aimed to administering hyperimmunized avian salmonella-immune lymphokines (S-ILK) to 200 one-day-old layer pullets to improve the immunological response against Gumboro (IBDV) divided into four groups. On the first day, the following was applied to all groups: G1: intraperitoneal injection of 0.50 mL S-ILK followed by a 30-min...

The aim of this study was to investigate the effects of probiotics on cognitive function and the regulation of cortisol and IL-1β in adolescents with depression. All 180 participants were randomly assigned to a study group (treated with probiotics combined with sertraline hydrochloride) and a control group (treated with sertraline hydrochloride). T...

Objective: Primary biliary cholangitis (PBC) is an autoimmune disease of liver that may be associated with other conditions, including autoimmune thyroid diseases. We aimed to investigate the frequency of anti-thyroperoxidase antibodies (TPO-Ab), antithyroglobulin antibodies (TG-Ab), and anti-thyrotropin receptor antibodies (TSHR-Ab) in Tunisian p...

Our study was to investigate the effects of bivalent COVID-19 booster vaccination during pregnancy on neutralizing antibody (Nab) levels in maternal blood (MB), transplacental transmission in umbilical cord blood (CB), and efficacy against Omicron SARS-CoV-2 subvariants including BA.5, BF.7, BQ.1, and XBB.1.5. We collected MB and CB from 11 pregnan...

Introduction Isthmin-1 (Ism-1) is a novel adipokine. However, little is known regarding the association between Ism-1 and type 2 diabetes mellitus (T2DM). This study aimed to investigate the relationship between serum Ism-1 levels and glomerular filtration rate (GFR) in patients with T2DM. Research design and methods A total of 209 patients with T...

The aim was to investigate the association between bisphenol A (BPA), 25-hydroxy vitamin D [25(OH)D], and 1α,25 dihydroxy vitamin D [1,25(OH)2D] levels in the cord blood of newborn babies. BPA was measured by high pressure liquid chromatography (HPLC) and vitamin D levels by commercial ELISA or ECLIA kits. BPA and Vitamin D levels were grouped acco...

The accumulation and aggregation of alpha-synuclein (α-Syn) are pathological processes associated with Parkinson’s disease, indicating that the regulation of protein is a crucial etiopathological mechanism. Interestingly, human serum and cerebrospinal fluid contain autoantibodies that recognize α-Syn. This potentially demonstrates an already existi...

El objetivo del estudio fue determinar la prevalencia de Brucella canis en una población de caninos vagabundos mediante diferentes técnicas diagnósticas disponibles en Chile y evaluar su nivel de concordancia. Se tomaron muestras de sangre y se colectaron los testículos de 30 caninos vagabundos de un sector de la zona oriente de la ciudad de Talca,...

A correlate of protection for rotavirus (RV) has not been consistently identified. Shedding of RV following an oral rotavirus vaccine (ORV) challenge has been investigated as a potential model to assess protection of parenteral RV vaccines. We previously showed that shedding of a challenge ORV dose was significantly reduced among recipients of a pa...

Yoga plays a role in reducing anxiety, depression and increasing serotonin levels in pregnant women. Yoga techniques aim to establish and maintain a healthy balance between all aspects of the body and mind. Prenatal Gentle Yoga is a solution that supports the safe and comfortable process of pregnancy to delivery. This research was conducted at Bara...

Giriş ve Amaç: Günümüzde hızla artan obezite sorunu nedeniyle gıda alımının kontrolünü sağlayan molekül ve yolaklar ile ilgili araştırmaların önemi giderek artmaktadır. İnsan dolaşımındaki temel pirimidin nükleozidi olan üridinin açlık ve gıda alımının dinamik olarak düzenlenen bir aracısı olduğu gösterilmiştir (1). Gıda alımını baskılayan Nesftati...

Avian leukosis (AL), caused by avian leukosis virus (ALV), is a contagious tumor disease that results in significant economic losses for the poultry industry. Currently, ALV-A, B, J, and K subgroups are the most common in commercial poultry and cause possible coinfections. Therefore, close monitoring is necessary to avoid greater economic losses. I...

Autoimmune diseases tend to cluster in families, suggesting genetic predisposition to autoimmunity associated with familial background. We have previously reported similarities in gene expression patterns and PTPN22 polymorphisms between alopecia areata (AA) patients and their healthy relatives, but not unrelated healthy controls. However, the spec...

Equine placentitis is characterized by infection and inflammation of the placenta. Different biomarkers associated with this inflammatory response have been evaluated in experimentally induced equine placentitis, but not in pregnant mares with spontaneous placentitis. The aim of the current study was to determine the concentration of eIL-1β and the...

Introduction: CagA protein, is a marker for the cag pathogenicity island which plays an important role in the etiology of H. pylori‑induced gastric pathologies. H pylori are detected in dental plaque, saliva and dental pulp. Saliva sample of 80 subjects with dental caries and free of caries is collected to detect the CagA level by ELISA technique....

Objective:In this study, it was aimed to investigate the relationship between the pineal gland and ghrelin and nesfatin-1 hormones in rats. Methods: A total of 36 male rats were used in the study, and the animals were divided into 4 groups. Group 1, Control; Group 2, Pinealectomy (Px); Group 3, Px+Melatonin; Group 4 Melatonin. After the end of the...

Steroid-responsive meningitis-arteritis (SRMA) is a predominantly Th-2 immune-mediated disease, but the exact pathomechanism remains unclear. Interleukin-31 (IL-31) is predominantly produced by T cells with a Th-2 phenotype during proinflammatory conditions. We hypothesize that IL-31 might be involved in the pathogenesis of SRMA. IL-31 was measured...