ELISA - Science method

Novel automatable specific clotting assay for TFPI. Measures kinetically the effect of TFPI on the extrinsic pathway. I a small cohort of patients with early prgany loss 10 in 13 samples were abnormal. Good correlation with free TFPI ELISA in many samples.

Ten recombinant antibodies (ABCD_RB859-868) detect by ELISA a peptide from the Dictyostelium discoideum DstA protein. Three of these antibodies, ABCD_RB861, ABCD_RB863 and ABCD_RB865, also recognize by western blot the endogenous DstA and the GFP-DstA protein stably expressed in D. discoideum cells.

This research introduces a novel method for controlling mouse movement with a real-time camera. Adding more buttons or repositioning the mouse's tracking ball are two common ways. Instead, we recommend that the hardware be redesigned. Our idea is to employ a camera and computer vision technologies to manage mouse tasks (clicking and scrolling), and...

Introduction: Data on the correlation between SARS-CoV-2 neutralizing activity and strain-specific anti-RBD IgG antibody (anti-RBD) titers is limited, particularly in the context of XBB.1.5-adapted vaccination. Methods: A direct comparison of neutralizing activity, measured as 50% neutralization (NT50), and anti-RBD titers, measured using an ELISA,...

Cortisol is a key hormone involved in stress responses, and its regulation can vary across populations experiencing different environmental pressures. While short term changes in certain environmental factors can result in immediate changes in cortisol levels, it remains unclear whether variable and fluctuating environments affect lifetime stress r...

Amaç: Çölyak hastalığı, özellikle genetik yatkınlığı olan kişilerde çavdar, arpa ve buğday gibi tahılların içinde bulunan glütenin neden olduğu bir hastalıktır. Sistemik otoimmün bir hastalık olan çölyak hastalığının prevalansı ortalama % 0,5-1 gibi bir orana sahiptir. Tanı için yapılan serolojik testler yetersiz kalmakta ve kesin tanı için biyopsi...

Original Research Article The purpose of this study was to ascertain the impact of interleukin 6, CD4 + T cell, and CD8 + T cell counts in albino rats that were fed bark extract from Cinnamomum zeylanicum. Seven albino rats were randomly assigned to each of the two groups (n = 7); group 1, or Control (C), was fed only regular food. For 28 days, Gro...

Photobiomodulation (PBMT) has been investigated as a non-invasive approach to modulate inflammation and muscle recovery, especially after intense exercise. This study aimed to evaluate the impact of PBMT on the dynamics of monocyte subpopulations and cytokine levels after a plyometric exercise session in physically active men aged 18–25 years. This...

AMP‐activated protein kinase (AMPK) is the master regulator of cellular and organismal energy homeostasis, playing an essential role in modulating metabolism and other cellular processes. Substantial efforts have been made to develop pharmacological modulators of AMPK activity due to their therapeutic potential against various diseases. Measuring A...

Regular monitoring of susceptible animal species for specific antibodies is essential to achieve or to maintain disease-free status for a country. The absence of certain disease in a country for many decades would yield expectation that collected animal serums would be negative for the presence of specific antibodies. However, large-scale tests oft...

Introduction: A cross-sectional study was carried out including 93 people between the age group of 30 to 40 years, who had sleep disturbances to a chronic level, to evaluate whether the lack of sleep really affects the body, and if yes, then to what extent does it affect. This study was conducted in Maharashtra. Method: The study was conducted amon...

Las cianotoxinas son producidas por cianobacterias que pueden encontrarse en agua continental y marina, son importantes para la salud pública por su potencial hepatotoxicidad y afectación a los ecosistemas acuáticos; la microcistina-LR es una toxina producida porciertas cianobacterias que pueden estar presentes en cuerpos de agua, por lo que es imp...

Background: Breast cancer is one of the most common malignancies in women, and its progression can be attributed to a variety of genetic and environmental factors. Objectives: To evaluate the impact of CEA, Mammaglobin and Resistin biochemical function in Iraqi patients with Breast cancer. Subjects and methods: A case-control study on serum samples...

Introdução: a dengue é uma arbovirose endêmica e de extrema importância que afeta a saúde pública por estar em expansão, assim objetiva-se expor a calamidade dessa doença em território brasileiro para conscientização popular e queda de infecções. Metodologia: esse estudo transversal de caráter observacional descritivo analisou dados do DATASUS como...

Purpose This study aimed to investigate the time course of β-endorphin (β-EDP) levels in healthy males following an acute exercise, while considering their age and physical fitness (PF) level. Methods Participants (N = 29, 20–65 years) were categorized in good PF young adult (YA1), excellent PF young adult (YA2), and excellent PF middle-aged adult...

La toxoplasmosis es una enfermedad parasitaria causada por Toxoplasma gondii, con alta prevalencia en Latinoamérica debido a factores ambientales, socioeconómicos y sanitarios. El objetivo de esta revisión fue analizar la epidemiología y los factores de riesgo de la toxoplasmosis en la región. Se realizó un estudio de revisión bibliográfica con enf...

Assay of steroid hormones in hair has become an attractive alternative for studies focusing on the perinatal period in equine medicine. The aim of the present study was to evaluate mares’ and foals’ hair ALLO concentrations and their ratio in relation to clinical conditions and selected clinical parameters. The 37 mare–foal pairs were categorized i...

In our previous study, a strain of Pseudomonas composti ODT-54 was isolated from the oyster digestive tissues. This strain is capable of producing Psl exopolysaccharides (EPS), which are directly involved in the specific binding to norovirus GII.6 P proteins. Here, we constructed an ODT-54 Psl EPS overexpression strain ODT-54 psl⁽⁺⁾ by substituting...

Objectives: This study aimed to investigate serum Maresin-1 (MaR1) levels among obese, overweight, and normal weight groups, as well as to evaluate their association with various metabolic parameters, including insulin resistance related indices and lipid profiles. Methods: Ninety subjects were classified into three distinct groups in terms of body...

Introduction Listeria monocytogenes causes zoonotic listeriosis with a high mortality rate, which is frequently detected in slaughterhouse processing environments and animal-based food. To enable the specific, rapid, and cost-effective detection of L. monocytogenes in environments and animal-based food, we developed a double-antibody sandwich quant...

Background and Objectives: Cerebral vasospasm is the most common complication of subarachnoid hemorrhage (SAH) that is related to high mortality and morbidity. Early biomarkers predicting those conditions are still limited. This study aims to analyze spectrin degradation products (SBDPs) as potential biomarkers for SAH patients, which can be used t...

In this study, a nanogold lateral flow immunochromatographic test was developed for the detection of rabies antibodies using a panel of well-characterized clinical and experimental serum samples. The lateral flow immunochromatographic rabies virus antibody test underwent a comprehensive evaluation, including an assessment of its limit of detection,...

CM7 anti-IgM Specific MAb useful to specifically titrate IgM in Plasma or Serum of MANY different Mammalian Species. ELISA data demonstrating efficacy of the CM7 MAb reagent on 28 different species from human through rhinoceros to hyena, raccoon dogs to flying foxes. This anti-IgM MAb simply does not work for cloven-hoofed mammals.

Background: Retinol binding protein 4 (RBP4) is the plasma carrier of retinol that complexes with transthyretin (TTR). RBP4 is a potential biomarker of cardiometabolic disease. However, RBP4 quantitation has relied on immunoassays and western blots without concurrent measurement of retinol and TTR. A liquid chromatography-tandem mass spectrometry (...

Background Human T cell lymphotropic virus type 1 (HTLV-1) is highly endemic in Brazil, necessitating surveillance studies to understand its epidemiology. While previous research has focused on either specific cities or populations, there is a need for multicenter studies encompassing epidemiologically relevant populations to ascertain more accurat...

Background The longevity of scrub typhus IgM and IgG are not clear. A region-specific diagnostic cut-off for enzyme-linked immunosorbent assay (ELISA) is a necessity for improved diagnosis. Objectives To determine a region-specific optical density (OD) value cut-off for scrub typhus IgM and IgG ELISA and to study the persistence of these antibodie...

AIM: To assess the expression of IRS 1 values in Oral Squamous Cell Carcinoma (OSCC) patients and its association with histological grading, tumor staging, and habits. OBJECTIVE: To assess the expression of IRS1 in patients with oral squamous cell carcinoma. To correlate IRS1 values with histological grading, tumor staging, habits, and overall surv...

Background The origin of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remains unknown. However, it is likely that the virus spillover occurred from an animal reservoir to humans. Identifying animal species susceptible to SARS-CoV-2 is crucial for understanding cross-species transmission to humans. This study distinguishes itself by...

Hepatitis B adalah penyakit infeksi hati yang disebabkan oleh virus hepatitis B (HBV), dapat menimbulkan komplikasi serius seperti sirosis dan kanker hati dengan penularan melalui kontak dengan cairan tubuh yang terinfeksi, seperti darah, air liur, cairan vagina, dan sperma. Pada saat terjadi inflamasi, sitokin yang ada dalam tubuh akan merespon at...

Background: Captagon (Kat) and crystal methamphetamine (crystal) are highly addictive stimulants that are widely used in Iraq. The neurochemical effects of these substances were examined in this study by comparing dopamine and total protein levels in users and a control group. Methods: Blood samples were obtained from thirty control persons, fifty...

Background and objectives- Occult Hepatitis B Virus infection (OBI)is defined as a condition when HBsAg virus infection is undetectable in serum, despite the presence of HBV DNA in liver or blood. This study was done to screen for occult hepatitis B infection among patients with CLD (chronic liver disease) attending gastroenterology OPD of tertiary...

Objetivo: Describir el espectro clínico – laboratorial, neurofisiológico y de neuroimágenes de pacientes en edad pediátrica con diagnóstico de encefalitis autoinmune (EAI), los inmunofenotiposencontrados, de acuerdo con las características clínico-inmunológicas encontradas, así como la evolución y pronóstico luego de la intervención con la administ...

Factor H-related (FHR) protein 1 and 2 form dimers resulting in FHR-1 and -2 homodimers, and FHR-1/2 heterodimers. Dimerization is hypothesized to further increase their antagonistic function with complement regulator factor H (FH). So far, only FHR-1 homodimers and FHR-1/2 heterodimers could be quantified in a direct way. With the reported genetic...

Corydaline (Cory) is a naturally extracted acetylcholinesterase inhibitor. In this study, we aimed to explore the possible roles and functions in Parkinson’s disease (PD) using 1-methyl-4-phenylpyridium (MPP⁺)-induced human neuroblastoma SK-N-SH cells. SK-N-SH cells were treated with MPP⁺ to mimic PD in vitro model. MTT assay and EdU assay were con...

Toxoplasmosis, caused by the parasite Toxoplasma gondii , affects approximately 30% of the human population worldwide. Reactivation of latent infection in immunocompromised people leads to fatal disease. This study aimed to determine the seropositivity of toxoplasmosis by enzyme‐linked immunosorbent assay (ELISA), confirmed by primary polymerase ch...

This study aims to investigate the differential level of CITED2 and PRX4 in the serum of preeclampsia (PE) patients and to explore their clinical value in PE diagnosis, severity assessment, and pregnancy outcomes. A total of 110 singleton pregnant women with PE were included, consisting of 57 cases of mild PE and 53 cases of severe PE, with 110 hea...

Background A number of studies have demonstrated that legumain is engaged in the pulmonary diseases. Nevertheless, the role of legumain is indistinct in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD). The aim is to identify the correlation of serum legumain with AECOPD patients through a prospective cohort study....

Background: Aging is often associated with progressive loss of function in various systems, including cognitive function. Impairment Cognitive Function (ICF) is thought to be associated with low folic acid levels in the blood, which is often found in the elderly. A cure for cognitive impairment has yet to be found. Measurement of folic acid levels...

Background and Objectives: Obesity has become one of the most significant health problems nowadays, with its prevalence rapidly increasing. Approaches such as diet and exercise play an important role in the treatment of obesity. This study aimed to investigate the responses of uric acid, irisin, adiponutrin, adropin, and copeptin levels to exercise...

Tuberculous meningitis (TBM) is the most serious type of tuberculosis infection, and there is a lack of accurate diagnostic targets for TBM. Therefore, it is of great clinical and public health significance to find a specific target for early diagnosis and prediction of the prognosis of TBM. From January 2021 to February 2024, 110 TBM patients and...

Annotatsiya Kirish. Pnevmoniya nafaqat bolalar, balki kattalar uchun ham xavflidir. Bu, birinchi navbatda, og‘ir asoratlarni rivojlanish xavfi bilan bog‘liq. Bolalarda eng keng tarqalgan kasalliklardan biri pnevmoniya bo‘lib, u kasallanish va o‘lim darajasi bo‘yicha yuqori o‘rinni egallaydi. Ushbu ishning maqsadi: bolalarda xlamidiyali pnevmoniya u...

Neutralizing antibody titers have been found to be strongly correlated with observed vaccine effectiveness against symptomatic and severe COVID-19. Few non-high complexity assays are currently available to detect the presence of neutralizing antibodies. This retrospective single-center cross-sectional study compared the performance of a lateral flo...

Immunoassays represent sensitive, easy‐to‐use, and cost‐effective tests useful for the detection of the SARS‐CoV‐2 virus. In this manuscript, we report on the binding specificity of a pair of novel monoclonal antibodies (MAbs) generated against the SARS‐CoV‐2 nucleocapsid protein (NP) and their development into sensitive sandwich enzyme‐linked immu...

Background Foot‐and‐mouth disease (FMD) is highly contagious and results in a high economic loss in the world. Methods A cross‐sectional study was conducted from December 2021 to June 2022 in three selected districts to determine the sero‐prevalence of FMD. A total of 384 cattle sera samples were collected and tested for antibodies against FMD vir...

Diabetes mellitus type 1 (insulin-dependent) (T1DM) is characterized by the selective destruction of the insulin-producing pancreatic beta. Asprosin and Gremlin-1 may have roles in T1DM, however these roles are not fully established. The expression of Asprosin and Gremlin-1 genes and their protein levels, was considerably higher in T1DM patients’ b...

Background: The aim of this study was to investigate the effects of different amounts of jackfruit pulp extract (JPE) in the diets on serum nesfatin-1 and visfatin levels in Streptozotocin (STZ)-induced Type 1 diabetic rats. Methods: In this study, 64 adult male Wistar albino rats, aged between 8-10 weeks, were used. 8 groups were created, with 8 r...

Tick-borne encephalitis virus (TBEV) is an emerging pathogen that initially causes flu-like symptoms and can progress to central nervous system (CNS) infections. Tick-borne encephalitis (TBE) is an endemic disease in southern coastal counties with regular human cases, while the causative agent, TBEV, is prevalent in ticks in most of the coastal reg...

Plaque psoriasis (PP) is a chronic immune-mediated skin disorder with a genetic basis, characterized by abnormal T-cell responses. This study investigated the role of FOXP3 gene variants rs2280883 and rs3761548 in T-cell regulation through their effects on IL-10 and TGF-β1 cytokine levels and their association with PP risk. A case-control study was...

Background Differentiating between viral and bacterial respiratory tract infections in pediatric patients remains a significant diagnostic challenge, often leading to the overuse of antibiotics. Myxovirus resistance protein A (MxA) has been identified as a promising biomarker for viral infections. This study aimed to assess the fluctuations in bloo...

Background: Recurrent Aphthous Stomatitis (RAS) is a common, painful condition marked by recurrentoral ulcers, impacting quality of life. Its etiology is unclear but involves genetics, immune dysregulation, andenvironmental factors. This study explores the link between serum C-reactive protein (CRP) levels and RASto assess the role of systemic infl...

El objetivo del estudio fue relacionar el cortisol sérico de mulares manejados en condiciones del trópico, con la actividad que realizaban y con el grupo de edad y sexo. La fracción sérica de muestras sanguíneas se obtuvo de 97 mulares dedicados al trabajo agropecuario (carga, vaquería y silla) y fueron analizadas a través de ELISA comercial tipo s...

Coronavirus disease 2019 (COVID-19) is a highly contagious infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) responsible for millions of deaths and substantial morbidity worldwide. Several studies report that up to 50% of individuals who recover from acute SARS-CoV-2 infection experienced a plethora of long-C...

Galectin-3 is recognized as a valuable biomarker for a range of pathologies, including heart failure, chronic kidney disease, and cancer. This study aims to investigate whether Galectin-3 can also serve as a reliable biomarker for vascularized composite allograft rejection in a swine model.

Objectives This study aimed to evaluate the urinary level of soluble CD163 (sCD163) in patients with type 2 diabetes as a potential early indicator of diabetic nephropathy (DN). Methods This cross-sectional study included 60 type 2 diabetic patients and 20 healthy individuals. Patients were divided into three groups according to their albumin/crea...

Background: Recurrent aphthous stomatitis (RAS) is an inflammatory nature, while interleukin 17 (IL-17) has a pre-inflammatory role. In the present study, serum level of IL-17 was measured in a group of individuals with RAS, compared to healthy individuals.Methods: This case-control study was done on 36 patients with RAS as cases and 36 healthy sub...

Neoadjuvant chemotherapy (NACT) influences the anticancer response by favourably altering the immune microenvironment. However, the effects of NACT on peripheral monocytes and their prognostic contribution to the NACT response have not yet been clarified. We aimed to evaluate the potential therapeutic responses and possible predictive value of doub...

Toxocariasis is a zoonotic parasitosis. The purpose of the study is to monitor the effect of Albendazole therapy by analysing clinical and laboratory indicators before, during and after its administration. Material/Methods: The subjects of the study were 34 children and 38 adults, with confirmed toxocara infection. Clinical symptoms and changes in...

Background/Objectives: Volleyball training camps are known to reduce salivary secretory immunoglobulin A (s-SIgA); however, when it begins to decrease is unclear. The validity of a simple device for quantifying s-SIgA is lacking; hence, this study aimed to observe detailed s-SIgA changes during a volleyball training camp after moving to a high alti...

Leprosy is a granulomatous disease affecting the skin, mucous membrane, and peripheral nerves. Irisin, a novel protein, has been associated with several inflammatory and metabolic diseases and has been demonstrated in peripheral nerve cells. The objective of this study was to compare the plasma irisin levels of newly diagnosed leprosy patients with...

Rapid assessment of severity is crucial for timely intervention and improved patient outcomes in heatstroke (HS). However, existing biomarkers are limited in their accuracy and accessibility in ER settings. A prospective pilot study was conducted to assess urinary liver fatty acid-binding protein (L-FABP) levels using a point-of-care testing (POCT)...

Foot and mouth disease (FMD) is a contagious and economically important viral disease of cloven hoofed animals caused by FMD virus. This retrospective study was conducted to determine the spatio-temporal distribution, estimate the morbidity and case fatality of FMD outbreaks in Western Amhara region of Ethiopia from January 2018 to June 2023. The F...

Measure Aβ42 levels in human neurons.

Pathogenic microorganisms responsible for infectious diseases pose a significant global threat to human health. Existing detection methods, such as qPCR and ELISA, fail to simultaneously meet the requirements for high sensitivity, high specificity, and rapid detection. This study presents an innovative approach for the rapid, specific, and highly s...

The enzyme-linked immunosorbent assay (ELISA) detects antigen-antibody interactions by using enzyme-labelled conjugates and enzyme substrates that generate colour changes. This review aims to provide an overview of ELISA, its various types, and its applications in detecting metabolites in biological fluids. The article discusses the history of the...

Early predictive markers of bone resorption are required to plan intervention strategies ensuring a healthy aging. Collagen I C-telopeptide (CTX) as bone activity marker shows a fair biological variability, that cause the lack of a well-defined reference range. Given the well-established role of vitamin D in bone remodeling, the aim of this study w...

Invited lecture as a resource person for ELISA

Background: Rubella is a mild exanthematous disease of global distribution. However, there is a risk of infection to the foetus subsequently causing congenital defects, if it infects susceptible pregnant women. Aims & Objective: To detect seroprevalence of Rubella in women with Bad Obstetric History. Materials & Method: The present prospective stud...

Background: The phenomenon of breakthrough infection during the COVID-19 pandemic has raised concerns regarding the efficacy of the vaccinations and healthcare providers are exposed to instances on a regular basis for professional reasons, putting them at risk. This study was aimed to look into the SARS-CoV-2 IgG status among the healthcare provide...

Background: Pyelonephritis is a serious and widespread infection of the kidneys, commonly caused by Gram-negative bacteria.This study aims to evaluate the immunomodulatory roles of sST2 and CXCR3 in the blood serum of patients suffering from acute and chronic pyelonephritis. Objectives: The main objectives of this study were to: Investigate the ser...

(1) Background: In recent years, there has been a growing interest in understanding the innate immunity of the mouth, particularly the mechanisms through which vitamin D influences oral health. Researchers have increasingly focused on the association between vitamin D and the antimicrobial peptide LL-37 since the CAMP gene, responsible for encoding...

Defects in motor protein-mediated neuronal transport mechanisms have been implicated in a number of neurodegenerative disorders but remain relatively little studied in Alzheimer’s disease (AD). Our aim in the present study was to assess the expression of the anterograde kinesin superfamily motor proteins KIF5A, KIF1B, and KIF21B, and to examine the...

The 10 recombinant antibodies ABCD_RB989 to ABCD_RB998 detect by ELISA the purified Dictyostelium discoideum GAA protein.

Nephrotic syndrome, the most common glomerular disease in pediatric population, is classified into two groups of steroid sensitive (SSNS) and resistant (SSRS). There is growing evidence on the role of T cells cytokines, including interleukin-13, in pathophysiology of nephrotic syndrome and steroid response. This study was a cross-sectional study co...

Rabies is an infectious but preventable disease with proper vaccination. Pre-exposure prophylaxis means using medications or vaccines against a given pathogen in people who have not yet been exposed to the given pathogen. Post-exposure prophylaxis means the use of medications in people who are exposed to a certain pathogen. This study aimed to eval...

Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies and leading causes of cancer-related deaths worldwide. Advances in HCC therapy have been significant but their ineffectiveness is often limited due to late diagnosis and poor prognosis. Early diagnosis is the key. AFP, currently widely accepted as a tumor marker for HCC, has l...

Background The SARS-CoV-2 virus, responsible for COVID-19, has the potential to induce the formation of antibodies that target self-antigens. This study aimed to investigate the prevalence of antinuclear antibodies (ANA), the most common type of autoantibody, in both healthy individuals and those recovered from COVID-19. Methods This retrospective...

The study aims to evaluate the significant role of interleukin 15 (IL-15), IL-22, IL-37, and Caspase 9 gene expression in polycystic ovary syndrome (PCOS), focusing on the underlying mechanisms and potential diagnostic or therapeutic implications. Peripheral blood has been collected, and serum was separated for the evaluation of the serum IL-15, IL...

The purpose of the study was to assess the levels of osteopoietin, copeptin, and malondialdehyde in diabetics. A control group of 100 healthy people between the ages of 55 and 75 comprised the study's 100 diabetic patients. ELISA was used to test blood samples. The results showed that individuals with diabetes had considerably greater levels of ost...

This study aimed to evaluate the effectiveness of the algorithm used in HIV diagnosis and to propose an effective new algorithm for rapid diagnosis. In accordance with CDC algorithm, our laboratory uses Architect HIVAg/Ab for screening and Geenius HIV1/2 and Artus HIVirus-1 QS-RGQ for confirmation. The Geenius test was used as a reflex and the HIV-...

Individuals with AIDS and Kaposi sarcoma (AIDS‐KS) with pulmonary involvement have high‐risk of poor outcomes but diagnosis of pulmonary KS in low‐resource settings is difficult. We aimed to discover plasma proteins that distinguish individuals with pulmonary KS from those without pulmonary involvement. SomaScan proteomics screen measured 7288 plas...

The label-free, all-optical biosensors, have been gaining more and more popularity in recent years. They can provide almost as high sensitivity as conventional techniques (like ELISA) in the detection of pathogens, although in a much faster and cost-effective way from some liquid samples. They are often combined with microchannels as sample holders...