Questions related to Drought Stress
This month due to solar Flair from the Sun has been surged forth into the planet earth, resulted to adding heat to extremely heated up planet earth, the heatwave caused drought and forest fires. Led to further devastating suffering and leafing deprivation.
Couldn't this forthcoming Solar flairs be planned to be capture to turn into energy use?
Sample Number Date Time O T100 K J I P S M T tFm Fo Fm Fv/Fm. Can anyone help me explain the J, Fo, and Fm parameters on the Chlorophyll Fluorescence measure and its significance to drought stress? I used a portable, pulse-modulated OS-30p+ chlorophyll fluorimeter (Opti Sciences). I am working on the effect of Alectra vogelii on Bambara groundnut.
My research investigated the impact of water stress on plant nutrition. Phosphorus content was stable regardless of increasing PEG concentration. Is there any valid explanation on why as i am trying to find the reason
I have studied the effect of ANE (Ascophylum Nodosum extract) on okra under drought stress. First factor is drought (control, mild stress, severe stress) and second factor is foliar application of ANE (Ascophylum Nodosum extract) (control, 0.1%, 0.2% and 0.3%), The experiment was conducted in pots using statistical design CRD (complete randomized design), same media was used. Drought stress, foliar application of ANE (Ascophylum Nodosum extract) and their interaction significantly effected physiological and biochemical parameters of okra. But the data recorded on control plants of drought, ANE (Ascophylum Nodosum extract) and their interaction is different from each other. Plants growing in control conditions are without any treatment.
Water scarcity is the universal problem both in developing and developed countries. Numerous researchers have discussed different strategies to cope with this problem. I am looking for the recent research outputs in areas where rainfall is scare.
We mostly associate drought with vegetative traits. I wonder if flower characteristics of dryland plants are shaped by drought. Is the presence or absence of the perianth and the type or structure of reproductive parts of a flower affected by this? I need sources on flower buds, flowers, fruit and seeds. Do you have any recommendation on this matter?
Is it that chlorophyll content and protein level are significantly affected under drought?
Do drought-tolerant genotypes produce specific secondary metabolites? If yes what are the secondary metabolites?
What should one look for (in terms of biochemical parameters) when studying drought tolerance or sensitivity in legume or cereal genotypes.
Please see the link below:
I am working on factors affecting shoot tips of rice under water deficit and I have some problems to arrange the treatments as moderate and hard drought.
I have a gene of interest (assume X) which plays role in many developmental events in plants. But I am trying to find its role in abiotic stress sepcifically drought stress. I have checked the expression of X in few different tissues where X express abundantly under drought stress (data available in database and reports) which is significant.
I was thinking to explore how X gene controls the photosystem when the plants are under stress. X gene is already reported to be involved in the regulation of photosynthetic genes using trangenics and other interaction assays.
Now, I am stucked in what way I can proceed. Could anyone provide me any suggestions?
I just want to know the proper use of these two words from the researchers who are working on drought stress
To study the effect of PEG in drought induction in perlite or soil, how and what concentration PEG 6000 can be used in citrus rootstocks of six month age?
We want to study the role of the lignin metabolic pathway in drought stress tolerance. Can anyone share their experience about how to determine the role of the lignin metabolic pathway in crops under drought stress?
For my PhD research project, I would like to call for your help in deciding whether to withhold water or to use PEG6000 for a drought stress experiment in tomato.
In fact, I would like to perform three levels of drought stress: control (well-watering), moderate drought stress and severe drought stress in pot culture.
On one side, PEG6000 would be easier to use even if some criticism has been raised in previous papers. On the other side, withholding water would mean coming to terms with the soil water retention curve, which is not so simple to compute (to me from scratch).
What do you think about it?
Thank you in advance.
Why there is larger interest in induction of drought in quinoa crop eventhough it's a drought tolerant crop?
We are using Specim IQ hyperspectral camera for image-based phenotyping in soybean plants at the vegetative stage. On processing the image on ENVI software, we got negative values for ARI1 and ARI2 in both drought stress and control. When I checked some published articles for reference, I found that the range for ARI is 0-0.2.
I want to find a drought stress in some plants. What are the other methods to find a drought stress other than polyethylene glycol-6000 method and irrigation method.
What are the chemicals that mitigate the drought stress in crop plants upon spraying? Also suggests suitable concentration for spraying.
Different approaches or methods that can be choose for inducing drought stress in Quinoa crop which is a hardy in nature?
Dear scientific fraternity !
I am going to do a research how to response 3 cardamom varieties at the drought stress in Sri Lanka. In here, polyethylene glycol 6000 used for increase osmotic effect. Then do a assay of catalase. Varieties take as a blocks with 5 replicants, different concentrations of polyethylene glycol take as a treatment and results of catalase enzyme absorbance take as a response variable.
The shoot growth is diminished during water deficit while there is increase in root growth in search of moisture and nutrients. How is it possible for root ?
I have visualized the alteration of the cell wall during drought stress with the confocal microscope. Now, I want to measure the cell size at different time points. Is there anyone who knows how many cells I should measure?
I have studied Arabidopsis at 7 time points (days 0,1,2,4,7,15)
We had indentified QTL regions associated with yield related traits under drought stress with the help of SNP markers.
Now i want to characterize this QTLs using flanking SNPs. So i want know what would be the suitable method used ?
How to find the candidate genes to validate their role through functional genomics experiments such as cloning, transgenics, over-expression, localization, and its interaction with other proteins and DNA, etc.
1)Do we need to study a lot of literature and see which genes role is not deciphered in particular traits e.g. drought stress?
2.) Do we need to perform our own transcriptome or comparative genomic studies or analyze already published studies from literature?
3. ) Do we need to perform our own marker traits association(QTLs) study or already published studies?
4.) Some people functionally characterize already known genes(say arabidopsis) to plant of their interest (legumes). But is it a significant or novel research problem to work upon?
5. all of the above.
I would like a more focus on urban areas and urban water use. I am interested to know experiences of drought management in different cities which are faced with drought for example.
The response of the physiological and morphological characteristic of three types of grass (Agropyron desertorum, Poa pratensis, and Festuca arundinacea) under drought stress
I want to know whether can we use inhibitors for certain specific group of genes like HSPs, to study their role in abiotic factors apart from validating the gene on heterologous expression system. Although, in case of animal system these inhibitors are widely used, but can we use it in non-modal plant system.
I would really appreciate if any information is provided.
I need to establish an experiment with sweet potato plants in relation to the effect of arbuscular mycorrhiza in the drought stress. Which kind of parameters are important to measure the drought stress in the treated plants ? stress
Hello! Actually I want study soybean physiology under stress in the field condition. Can you please suggest the probable techniques to create and maintain drought stress for plants.
I am currently working on antioxidant activity of drought stress plants specifically Catalase and Ascorbate peroxidase and most of the papers that I've read they quantify unit of enzyme activity in "mg of protein". Could you suggest other formula in determining antioxidant activity without determining the protein content?
It is known that some stresses cause changes in hormonal, genetic and cellular levels in the plant. In order to determine the physiological response of the plant in case of stress, it has been discussed by many researchers whether determining the proline content is stress-related. What do you think about this subject? Thanks for the comments
Hello dear everyone's
Can combination GA and PEG be used simultaneously to study drought stress in the seeds with physiological dormancy?
Thank you in advance for your attention
I am doing drought stress experiment and i want to know about the grams of yoshida powder i need to make the nutrient solution for the plant.
The duration and intensity of drought is increasing worldwide largely due to climate change.
Development and genetic improvement of drought tolerance crop become very important for the future due the effect of climate change.
What the best method for screening and evaluation drought stress on crop plants especially "rice"?
Thank you for the answer
Hello everyone, I'm working in Brassica napus under drought stress and oil contents in germination stage and I want to find relation between both of them so can any one tell me some genes related to this relation or how I can explain this relation.
I have Sorghum expression data related to drought stress that I obtained from the National Center for Biotechnology Information (NCBI), Expression Omnibus (GEO) database, I need to select the expression pattern of one gene family from this RNA-seq data, I have no idea how to do it, it would be helpful if someone suggested me how to do so. Thank you.
I am working on determining the starch, glucose, fructose and sucrose level in drought stress leaves of a forage grass for that I need the Papers in which the easy methods or assays are described about the above mentioned parameters. Thanks
The Dayaknese tribe, A tribe in Kalimantan island, was cultivated upland rice in high elevation of Meratus mountains.
Some cultivar was cultivated and not identify by researcher. Due their ability to growth with low water in the soil, these rice genotypes was expected have an ability to drought condition.
Interestingly, the rice grain was storage by the local people for a long time (up to 7 years) after harvesting time.
This information leaving a few question for me:
Is the upland rice genotypes important for drought tolerance?
Is the rice seed still viable after long time storage?
Thank you for the answer.
It is difficult to work on crops and chances of successful experiments are very less, that is why we prefer these model organisms to study the mechanism and then apply the same knowledge on other crops. So, according to you, for different traits and developmental studies which is better?
looking for method to induce drought stress with different intensity in pot with soil? Any suggestion are welcomed.
I am doing experiment to check increase in mycorrhiza colonosation with roots during water stress in maize. I am inducing water stress at 14 days after sowing, so when i should add mycorrhiza to maize pots
i am planning to add 7 days before inducing water stress? is it gives precise effect of drought on mycorrhizal colonisation?
I cannot seem to get direct pathways that shows how free radicals are produced in plants under drought stress? Any advice on articles that clearly explain the production of free radicals in plants under stress?
I will be study the gene expression related to drought stress in woody plants, and I'm confused for the method to apply the stress. It Can anybody tell me what is the best way to applicate water stress on the woody seedling.
Knowing that woody seedlings are planted in soil pots.
Did anyone use PEG in an aeroponic system? I wanna do an experiment to analyze some drought stress tolerance related genes in aeroponic condition, but based on our calculation we need a lot of PEG if we want to use it similar to hydroponic culture?
Is there different methods to induce drought stress?
Thank you in advance
The basis for ABA as an important signal is that both salt stress and water deficit can induce a rapid and massive accumulation of ABA in plant tissues. This process itself is a cellular signaling cascade, in which the perception of salt- or water deficit signal or the initial triggering for ABA accumulation is the most important step.
It is well known that the second messengers and protein kinases or phosphatases are pivotal signal components mediating various responses of plants to biotic and abiotic stimuli. It was suggested that Ca2+ might also play vital roles in water or salt stress-induced
ABA accumulation. However some studies reported that ROS were involved in stress-induced ABA accumulation.
I am investigating the response of single trees and whole stands to droughts by calculating resistance and resilience indices using the Basal Area Increment of the trees. I want to figure out how the response is influenced by the species composition of the stand. The data I have is single tree data from around 1930 to 2017 with the dbh, the species and also the altitude (which could also be investigated - the importance of altitude/latitude). I am not quite sure which method is the best here to calculate the diversity and I am very grateful for any suggestion/answer.
I'm looking for latest research articles on transcriptomics and proteomics study on transgenic sugarcane under drought stress
effect of drought stress on water use efficiency
effect of salinity stress on water use efficiency
Normalized Difference Vegetation Index (NDVI) & Normalized Difference Water Index (NDWI) are two remote sensing indices used to interpret the vegetation. What different interpretation do they offer about the vegetation? Where are they used in practice? What are the cases when NDVI and NDWI are not correlated?
hi everyone !
I'm trying to evaluate growth of a fungal isolate under drought stresses in vitro. For making that happen, i need to dissolve PEG 6000 in culture medium under axenic conditions but PEG 6000 polymers are Affected by temperature; Because of that i can not add PEG 6000 before autocalving. If you want to add it into a broth media it's easy because you can add it after cooling down then use 0.22 μm filtrates but if you do the same when you are dealing with a media that contains agar that is not going to happen.
does anyone have experiences with drought experminents in canola and knows how much the soil pF-Values need to drop to put modern hybrid winter varieties of canola into drought stress?
I have developed transgenic potato lines expressing a drought stress related gene. How can I confirm gene integration and expression of these lines?
As Jasmonic acid is volatile, so what would be the best way to keep its stable form during treatment in 1/2 MS plates?
And I also want to apply Jasmonic acid during seedling stage. So what would be the best way to apply them along with drought stress?
We are going to select more drought tolerant genotype among a population, which method is more reliable for impose drought stress for selection?
I am to use three chemicals (Glycine Betaine or Thio urea or salicyclic acid ) as foliar application in American cotton.
The application is aimed to minimize the high temperature or drought stress under North Indian conditions where currently temperature is 46 degree +.
Can these chemicals be directly mixed with water as tank mix spray or
Do I need to dissolve these chemicals with some other substance/chemicals so that they may be well mixed with common tap water.
Kindly update if you know about this.
I am looking to identify meetings and sessions in the field of xylem/plant water transport. If you have ever organized or attended a conference session or meeting dedicated to any aspect of this area of research please share the name of the conference. Any information is greatly appreciated.
Why Epigenetic Mechanisms are the key players in installing plant memory, while the genetic mechanisms comes second?
Thanks in advance.
The plant in the attached image is a wild type! It have been grown in pots and all growth conditions were in educate levels. Why does the plant still show symptoms of stress!
What is the role of these small white crystals?
Note: The plant with significantly salty taste.
When I conduct experiments for the evaluation of microbial inoculation effect on crops under drought stress, I get just agronomic evidences, but it would increase the quality of observations to know the amount of proline in plants.
Notice that accumulation of osmotic constituents like proline, and sugars during stress enable plants to maintain the osmoregulation and recover from the oxidative stress. Thank you.
In some cases the co-inoculation at the same moment gives great outcomes, but it seems that separate inoculation in different times is better when the experiment is conducted under stress environment, such drought.
As i came to know with different views regarding the range of flower drop in Mungbean genotypes.
What should be a general range for normal and stressed conditions both?
Is the artificial drought stress creating process same for pot experiment and field experiment?
I am working with maize drought tolerance. I am analyzing data under drought stress and normal condition. While doing data analyses I have found that traits like Anthesis silking interval, thousand kernel weight has a heritability of zero under drought stress condition. But when I combine data of both drought stress and normal condition heritability of those two traits is not zero. Is there any problem with my data? or is it explainable in other ways?
Looking forward to answers..