Science topic

Dogs - Science topic

The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
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If the cow is bitten by rabid dog is it safe to consume its milk (If there are any specific literature available kindly share).
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Not safe if the dog is infected with zoonotic diseases like PPR
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Dear all, I have to draw the complete pedigree of a high number (>40) of dogs in a study regarding genetic diseases, so I started drawing the pedigree using the R package kinship2.
The problem is there are many common ancestors between the tested animals, and as soon as I add the grand-parents of the subjects the tree gets all "scrambled up", with dashed lines connecting the same individual present more than once in the pedigree and drawn in different spots of the tree. I know that human genealogic trees are usually far more simple than this scenario and this package should work at best.
Manually adjusting the branches to avoid subjects duplication is quite hard to do, so I was wondering if there is an easier way using a different software (possibly free) or a different R package best suited for complex animal pedigrees.
Thank you,
Riccardo
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This is a good online tool. You have to register to use it.
Sometimes it will get scrambled up as you describe for kinship2 but that is solved by pushing the "RE-LAYOUT" button.
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Hi,
I am doing my MSc thesis and it is based on two questionnaires. I am comparing opinions on canine hip dysplasia of vet surgeons and CHD affected dog owners. Both groups had questionnaires with matched questions (for example owners had a questions: “at what age was your dog diagnosed with CHD?” & the vet questionnaire had a questions “at what age dog CHD is most commonly diagnosed”?) and same answers.
The problem is the number of respondents. I have 121 owner questionnaires and only 31 vet questionnaires. I am planning to do cross-tab and chi-square test, but not sure how to:
1. match two different surveys on spss;
2. compare the different response count.
any suggestion will be greatly appreciated
kind regards,
Bettija
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To carry out a inferential statistical test even groups isn't required. Parametric: You can use dunnett's test for unequal variances and fisher test and/or chi-square test of independence for equal variances. Non-parametric: dunn pair wise or dunn control
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One of my undergrads created a phylogeny based on few human hCov19 and few from other hosts including bat, panguline, and dog. He found two viral genomes one from an Australian and one from a Singaporean clustered with the one isolated from canine. This seems interesting! Any study to support this finding?
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Can anyone suggest any ensembling methods for the output of pre-trained models? Suppose, there is a dataset containing cats and dogs. Three pre-trained models are applied i.e., VGG16, VGG19, and ResNet50. How will you apply ensembling techniques? Bagging, boosting, voting etc.
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I've seen evidence of strange behavior among people who walk their dogs on trails in parks or seminatural areas in my suburb. People will carry plastic bags for picking up their dogs' feces but then, instead of discarding the filled bag in a bin, they will throw the bag away like litter, and often in the vicinity of the bins. (I've never actually seen anyone do this, just the evidence thereof.) This behavior creates a more serious pollution issue than not cleaning up after your dog since the plastic bags don't biograde. Have there been studies on this or similar counterproductive behaviors?
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I've just moved to another suburb, 20 or so km distant. Again the same phenomenon in the parks — people bagging their dog poop but then littering the park with the plastic bags of poop. What's the point? I just don't get it. If they took their dog off-trail to poop in the bushes it would at least be biodegradable.
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Which is a good method (model/system/individual stand) for individual feeding of dogs kept in group (8 to 10 animals in pen)?
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A pROBLEM! animals in a pen are no longer individuals due to competition. Thus, your PENS THEN ARE THE EXPERIMENTAL UNITS not the dogs...
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I'm looking for a dataset of natural images, with no specific requirement on the type of scene, designed for the task of detecting (automatically or visually) the presence and position of small objects. This could come for example from an eye-tracking experiment where participants are tasked to e.g. look for dogs in a picture taken with a camera, or an image segmentation dataset. The point is that the task is challenging/the category is rare/the segmentation area is small and hard to find. Each scene would ideally be associated to a label or set of labels indicating the object/s to be searched for, an explicit position or the segmentation masks are a plus but not required.
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Mask COCO dataset
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This questionnaire is for dog owners. This is part of my disseration for animal science. I am looking to see if canines with anxiety are more at risk of becoming ill. The data will only be used for the purpose of this disseration. even if your dog does not have any anxiety please fill in as it may just prove anxiety has no effect on a dogs health.
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I'll be interested to see the results, with the proviso that owners are not always the best judge of their dogs! Also, "gender" should be "sex".
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Is there any review paper or book chapter/book available on the topic Parasites in the nervous system of dogs and cats?
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Chapter 19 of small animal neurological emergency by Simon Platt and Laurent Garosi would be helpful .
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Hello,
After few years I was re-sequencing a fragment of the D-loop of dog mitochondrial DNA and I found a heteroplasmy which was not previously observed at the same position. In the attached picture there are two chromatograms of the same sequence from the same dog, from the same blood sample. The chromatogram above is from 2014 and the below one is from 2021. The isolated DNA is the same. The heteroplasmy was observed in several samples at the same position in other dogs' sequences, however in all sequences the quality is poor due to the double peaks in the baseline. I don't understand why the signal dropped only at one position, and suddenly raised in the next position.
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I would rather go with a NUMT. By targeting mitochondrial sequences with your primers, it is likely that they also match mitochondrial fragments inserted in the nuclear genome. The fact that you find it in several dogs and that several differences seem to have already accumulated would also point in this direction.
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Oral bait vaccines against rabies are nowadays used in wild animals.
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Dear Abrar, it appears that Prof. Aissa Saidi has a great experimental experiences and I agree with him. But, you You have to do a pilot study before applying the oral vaccination on the domestic animals.
Kind regards
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I need some articles about isolation of Klebsiella pneumoniae In dog mouth... Thanks
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If a calf say of 7 days old got bitten by a rabid suspected dog then what should be the course of action except euthanasia?
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We have given PEP to cows and buffaloes bitten by lab confirmed rabies dogs and mongoose with intradermal rabies vaccine and local wound infilteration of equine rabies immunoglobulins with 100% survival in Himachal Pradesh.
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I'd like to discuss some aspects about the hight sensibility in dogs, specifically in working dogs
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Dear Gloria Durán
I think you are asking about High sensibility in that context if am right below information will give insight about it
Dogs with very sensitive personalities may be more difficult to control, and problematic dogs may be disciplined more frequently. Positive punishment may not work as effectively for dogs with a very sensitive personality feature, therefore owners focus on negative punishment instead.
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The attached pictures are those of a swollen dog's ear.
We observed that the ear of our local dog is swollen without visible lesion, cut or scar.
However, it seem there isn't abscess or pus in the observed mass.
What could possibly be the cause and how can it be treated?
@vsamter
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An auricular hematoma, surgery would be recommended.
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Hello all
thanks alot for your support
I got up with a doubt , for simulation normally we use ansys or abacus of tensile testing . But when it come to polymers tensile testing which software need to be used. as the infill pattern will change the effect of fracture , what software we need to use for simulating tensile testing of specimen's. if we go with ansys the dog bone will be treated as simple structure but not infill parameters. can some one clarify my doubt please
need software suggestion to do testing simulation.
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i recommend MSC marc, in nonlinear FEM moddule
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Hello, I was wondering whether there is a non-invasive technique to apply a GPS collar to a wild dog. I was thinking about e.g. a tree hole with some food where they have to put the head through the (elastic) collar to get in, which then automatically attaches to the dog? Or maybe remote controlled (with the help of a camera trap) - like a reversed drop-off mechanism? Or anything else... Thanks!
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Dear Andreas, I'm also interested in this subject, as you suggest I was thinking of placing a collar on specific species path/food trap and use a "necklace trap system". Indeed, the animal will pull his head into the "collar snare" which will adjust the collar to the size of the animal's neck (with a safety catch to avoid any strangeness). Once the pressure is too strong on the cable/elastic used, the trap will break and the animal will be released with the collar. A safety system on the collar itself will have to be put in place to avoid a too important strangulation (elastic?). Or a system that wears out over time, allowing the collar to fall off after a while. The trap could be monitored by GSM camera trap to verify the proper conduct of the operation, in case of problem a rescue operation could be deployed quickly as the camera trap will have sent the images.
For other burrowing species, I thought of a tube at the exit of their burrow that would force them to borrow the latter. In the tube, a mechanism would allow a plate previously coated with special glue to stick to the back of the animal.
Of course the security of the animals must be the priority and these are only ideas to be developed and tested.
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For my college assignment I need to produce a leaflet detailing the information required when assessing a dog for a diagnosis for hydrotherapy and physiotherapy and the impact this has on the treatment. I was wondering if anyone could help me out?
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Here in the US, Pet physiotherapy or "physical therapy" is similar to humans, where knowing the diagnosis, disease course and prognosis determines what treatments are appropriate. If deemed appropriate (say for example: tendinitis/tendinosis) hydrotherapy can be a potential treatment option. However, you much take in to consideration pet preferences, and contraindications such as open wounds.
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We are having trouble locating an antibody that shares more than 70% homology with dog.
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Thank you very much for this information.
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Hello,
I've build a Conv1D model to classify items into 6 categories (from 0 to 5). (simplified example)
I use therefore a CrossEntropy loss function that penalizes the errors with high probabilities and also the low probabilities associated to right answers. And that is exactly what I was looking for.
But CrossEntropy considers the classes as independent, while I'd like reduce the loss for an error where model predicts 0 (cat) while it is 1 (dog) and increase the loss when the model predicts 0 (cat) when the true answer is class 5 (fish).
Is there a way to embed the 'class proximity' in the loss computation of a classification problem, so that a error between classes 0 and 1 will be less penalized than an error between classes 2 and 5 ?
Many thanks in advance for any help !
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Hello Kai,
THX.
I indeed implemented a custom loss function that is a variation of Cross Entropy Loss. It considers the difference between the distribution probabilities but than add a "penalty" for the distance between the classes.
I shared it in my github (JDE65).
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I wish to direct the researchers to think of using a dog's lung structure or cells to guide us how to develop a medicine or vaccine or treatment for diseases such as corona.
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Dear Vijay K Jindal thank you for this interesting technical question. According to the link cited below "A small number of pet cats and dogs have been reported to be infected with SARS-CoV-2 in several countries, including the United States."
COVID-19 and Animals
(Updated March 25, 2021)
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At University, we are doing an assignment on the development of certain behaviours from wolves to dogs. My friend made an excellent point. When wolves were domesticated, they were bred to remove aggression and to increase tameness. But when people now breed dogs for aggression, then they lose control over their dogs, lose the tameness (to an extent) there fore are almost "undoing" domestication with their dog.
I just wondered if there were any studies that have looked into this, or if anyone had any thoughs on the subject.
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This is a very interesting question, although i think that there are few other things to take into account. First of all, i would consider that with domestication of course the aggression was decreased but also the fear reactions towards us, so dogs were just more flexible not less aggressive per se. I mean, with decreased fear reactions, decrease aggressive behaviors came as well.
Also, i would always consider which kind of training and social experience are the dogs that you mention, facing. From my experience, abusive training, punishment, and bad socialization are more behind these behaviors rather than a "reversion of domestication". And again fear is involved, and we know that fear has a high hereditability rate :)
Very interesting, thanks for bringing it up :)
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I'm looking for a date range of when domestic dogs were brought to Ireland and who brought them there. The closest I've gotten is when the so-called "lap dogs" were brought in and there is no date for that. Yet I've found information that "Irish hounds" existed back in AD 393 so they had to have gotten there before then.
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Try this:
McCormick F (1991) The Dog In Prehistoric and early Christian Ireland, Archeology Ireland, V5(4), pp 7-9
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I am culturing MDCK dog kidney cells. I am trying to express shRNA to knockdown target genes. There are so many shRNA design tools for human, mouse and rat species. I haven't been able to find a good way for dog species. Does anyone know any good tool such as website or algorithm? Thank you in advanced!
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Thanks Phiyani! I totally agree with that we cannot avoid off-target effect with certain shRNA and it should be compensated by different shRNA targeting same gene. by the way, I'd like to get shRNA sequence targeting my gene of interest, which is predicted to have minimal off-target in dog genome and am wondering which algorithm or website is able to help me with this analysis. Do you have any idea by chance?
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I would like to test some markers to identify spermatogonial stem cells in cats. Should I choose the markers that will be tested from known human or dog markers? Which species will most likely have antigenic similarity to cats?
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Hi.
I think you can use known human markers that have been tested. Because human antigen is more similar to cat antigen.
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What is the purpose of this project?
• To investigate the factors affecting the rehoming rate of dogs.
Objectives:
• Questionnaire will be distributed to members of the public (over 18) for completion.
• Data will be stored in accordance with GDPR guidelines e.g. anonymous & password protected.
• Obtain data from various rehoming centres on successful rehomes and returns.
• Participants data will be analysed alongside data provided by rehoming centres.
• Thematic analysis and correlations between factors observed in rehoming data and analysed alongside questionnaire data.
See below for questionnaire link:
Any further informtion is provided before the questionniare (Should you participate)
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Ooh Scunthorpe - bet it's chilly up there! Good luck with the studies; I would have loved to have one mine full time at uni rather than whilst working full time to fund it.
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Dogs, properly trained, discover easily and infallibly if a person is infected with SARS-CoV-2 the virus that causes the COVID 19 disease. Is it possible to build an artificial nose to detect those infected with the virus SARS-CoV-2 as dogs do?
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interested
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I am interested in pursuing my masters thesis research on the use of Tilapia skin grafts in wounds of dogs. Any inputs would be appreciated.
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I following the best answer.
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Why animals are (cow, dog, goat etc.) matting only before breeding?
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Very Welcome ABDUL Aziz
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I read a few articles related infant & dog's auditory preference. They both mentioned "baby talk" could be more attractive to infant & dog. And my team and I want to find out the reason from evo-psych. We already know one of necessary condition is high pitch, and had some data about high pitch preference for dogs (we took gaze duration to be parameter).
Our project focus on evo-psych puzzle, so we want to exclude the physical or physiological reason to explain why dog prefer high pitch. I tried to do some reviews to know physical trait of high pitch. But I almost found nothing help because I barely know the domain knowledge of acoustics. So if physical trait of high pitch could explain why mammal like human and dog has such preference, then we should reschedule our project.
Thank you for response such fundamental question.
Sincerely, Jhou.
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Higher sounds (including out of the auditory range of humans) are also emitted by small prey animals which may supplement scavenging in feral dogs and engage prey drive even in companion dogs and those that have had prey-seeking traits bred out. They seem to engage the attention of most dogs which is why squeaky toys work.
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Toxocara are described as very frequent in dogs and certainly across the world overall prevalence of T canis is described as 11.1% in a paper by Ali Rostami and others in Advances in Parasitology Volume 109(2020) pages 561-583. However I can find no recent papers of the prevalence of this parasite in the UK (I am not working in academia and do not have access to all references). This is important because the use of potent anti-parasitic treatments often on a monthly or three-monthly basis is extremely widespread in the UK. Some anti-parasitic drugs leak into waste water or the wider environment and may be causing eco-toxicity effects on insects soil nematodes etc.
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Hello
I am doing my Psy.d on the social validity of the intervention of animal assisted therapy (dog) on a PTSD population in the province of Quebec. The only close tool of measure I found is the treatment acceptability ans adherence scale (wich was made for anxiety at the base).
Anyone is aware of a complementary tools that I could use.
Thank you
Valérie Bédard
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I was interested in your question, which led me down a rabbit hole of literature searching. Social validity is a really interesting construct, and one that has been a major concern of mine in my own research, though, comme M. Jourdain, I was unaware that what I was aiming for was called social validity, sans que j'en susse rien!
I found this article helpful : http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.565.3507&rep=rep1&type=pdf. My feeling is that you will have to define the appropriate outcomes and measure them. By definition, almost, a particular intervention in a particular society/social group/context will have particular objectives that must be (I think) defined in partnership with the group. No alignment, no validity. This holds out the exciting possibility that the group, not the researcher, are the people who both define the outcomes and measure them. In this way, if the research works, it gains social validity through both partnership and communication of knowledge.
When I set up the original solar disinfection trials among the Maasai, the principle was that the trials would be in Maasai, all field work would be conducted by Maasai and all findings would be communicated back through community meetings. The trials were a success but for me the big win was the pride that the community had in something they had discovered for themselves.
Interesting topic – good luck with it!
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Hi everyone,
I have transcriptome sequences of parasites found in dogs. I use the blood from the dog to amplify the parasite so I would like to make sure that the sequences that I am using are of the parasite and not the dog?
Which bioinformatics software can I use and how do I go about it?
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Dear Keneilwe,
The best way to proceed is to take your sequence(s) and to carry out a BLAST search against the NCBI nucleotide databases (https://blast.ncbi.nlm.nih.gov/Blast.cgi). There are different types of BLAST programs depending on the query sequence (protein or nucleotide) and the database to be searched (protein or nucleotide). The search output gives you the most closely related sequences together with their alignments with your query sequence. Sequences of interest can immediately be downloaded for further analysis. How to search and how to interpret the the search results is discussed in the following chapter: Chapter Phylogenetic analysis using protein sequences
Don't hesitate to contact me in the case you have any questions,
Good luck,
Fred
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While doing my research work, I have noticed that the D- dimer levels of normal animals (dogs) are given in different ranges in different publications. Is there any variation occuring in d dimer levels due to the application of different methods? . And what is the normal serum D-dimer levels in dogs? I am so confused with the reference range of serum D - dimer. Thank You in advance.
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If difference in reference values are greatly pronounced, it could be due to some adjustments made prior by researchers. Otherwise, it is just in method of preparations that they vary. Researchers tweak these variables that suits to their needs.
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Has anyone measured stress in dogs?
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Yes
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I want to measure HRV in dogs with a Polar H10 belt. But I can´t get a good signal. I tried EEG Gel and shaving but the results were not great. Any advice on how to improve the signal?
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PetPace smart collars for dogs measure HRV every 2 minutes and had been validated and used in behavior studies. See for example -
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Dr. Caleb Bryce and I have organized a canine science symposium for the Society of Integrative and Comparative Biology (SICB) meeting January 3-7th, 2021 in Washington DC (http://www.sicb.org/meetings/). Attached you may find a poster with a list of the speakers for our symposium which will be held January 7th, 2021.
Abstracts will be due late August to early September 2020. You do not have to be a SICB member to attend this meeting, and you can also register to attend for a single day.
If you cannot attend part or the whole meeting, may I ask that you help us spread the word about it by forwarding this information and poster to potentially interested parties?
Our hope is that dogs take over SICB 2021, hopefully with your help!
Please do let me know if you have any questions.
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The Society for Integrative and Comparative biology is actually taking more abstracts until October 28th, 2020. And, we are still looking for people to participate in the complementary session to our canine science symposium, so your work is still most welcomed!
To submit your abstract, please go here: http://sicb.burkclients.com/meetings/2021/abstracts/late.php
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My question is about ''Can the bacteria Capanocytophaga canimorsus cause the infections in dogs?''
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It is a commensal bacteria in dog which causes disease in humans but very rarely in dogs themselves.
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Dear Researchers,
I'm a veterinarian who ended up in the bioinformatic world as I am analyzing the RNA-Seq using samples from colons of 2 groups of dogs; 1 control group of 3 beagles where I have sent 3 colonic samples with histopathology as normal colon mucosa and 1 disease group of 3 dogs where I have sent 1 set of diseased and normal colonic sample for each dog with histopathology as diseases and normal colon mucosa. Basically I have 3 replicates from 2 groups of dogs, but with 3 different conditions (1 condition of normal dog colon, 1 condition of diseased dog with diseased colon, 1 condition of diseased dog with normal colon) making it up to 9 samples, which warrants the use of edgeR since I have less than 12 samples.
The colonic RNA samples were sent for RNA-sequencing in another DNA research lab and I honestly am not sure which protocol they have used; the information received was the sequences were proceed using CLC genomics workbench 12 to produce the excel file with total count, RPKM, TPM and CPM.
Machine used was Illumina HiSeq 2500 with single-read,50bp library; and sequenced with 15 million reads, where FASTQ file was retrieved.
The flow of RNA sequencing was done as below:
1. From FASTQ reads, the Illumina Adaptor was removed
2. From FASTQ reads, the unreliable bases were removed
3. The reads were mapped on the CamFam reference
4. RPKM was calculated.
I am in a dilemma now if I should re-process the raw FASTQ files myself, or by just using the current Excel file; as I do not have access to supercomputers in my facility.
I am trying to analyze the excel output using Rstudio with the EdgeR package for normalization and hopefully DGE analysis, but I'm pretty unsure of at what point my current excel data is at. Will I need to normalize it, or should I not be using the Excel but the raw FASTQ for the EdgeR instead (as the user manual says to not use FPMK in the EdgeR, but the raw data instead). Will total count and total read numbers be suffice to come out as a raw data for EdgeR?
I have up to 20,000 genes to analyze, I wonder if I do have sweet time to start from raw data.
Thanks everyone!
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Alex G Lee thanks for the reply and suggestion! My supervisor told me something pretty similar so we ended up using a commercial program called StrandNGS to perform the alignment and running the edgeR script within the program to get our results.
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Dogs are capable to. heat ultrasound frequency
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Hi again, Satish,
The other company that makes reliable commercial bat detectors is Pettersson Elektronik. This is a company specializing in electronic equipment for detection and analysis of bioacoustic ultrasonic signals. With their ultrasound detectors, or bat detectors, you can listen to the ultrasonic calls from bats, a great aid in identifying different species. Their website is:
I hope this is helpful.
Peter
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The sample cannot be reformed to the dog bone shape for testing purposes.
I have tried to stick sandpapers with epoxy to the ends of the specimen but it still slipping .
roughening the ends of the sample doesn't prevent the slippage aswell!
sample thickness cannot be reduced!
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Cellulose and protein are more slippy. Any anti-carb or anti-protein reagent can be helpful. Consult with a Biochemist/Chemist.
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Elements of Ca, K & Mn were detected in cystolith from a 3 year old male Dalmatian dog by EDXRF method. Can anyone tell me which cystolith it is?
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So far I know, CALCIUM.
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Hello,
is there anyone working with embryonic stem cells from dogs? Are they commcercially available?
Thank you very much
Christiane
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So far I know, this is not available. Selective breeding is sufficient in this case.
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I try to culture Trichomonas tenax from dog's mouth in Diamond's medium. I have used both home-made and commercial media. I have live cells isolated from a few dogs in these media. However, they all die off after being diluted into new medium. What have I missed? Any advice is greatly appreciated.
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As a culture media- milk, egg, and blood are excellent.
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Hallo, i'm working on small university project on Human Visual Attention cues and how dog use this to change their Behavior. My question is: what is the Attention Cues used between dogs in a their social Behavior? Such as when the dogs going around a place grouped, how this dogs interact with other dogs? I think the this topic lays the foundation of "Human Visual Attention cues and how dog use this to change their Behavior".
thanks,
Domenico M.
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Attached article can help.
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Do you make one trust or more? and where? Only dog position?
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This position helps for close affiliating.
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dog as type 1 diabetes model 
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Diabetes mellitus is nothing but domestication.
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I am beginning to compile a methodology for testing personality in domestic dogs for a dissertation looking at the role that personality plays in determining the success or failure of assistance dog training.
At this stage, I am considering comparing working Labrador retriever assistance dogs for the blind, failed trainees that have been rehomed and a control group of companion dogs.
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Human body spray can be helpful.
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I can't find many studies regarding gallbladder emptying times in dogs. In particular, I wonder if this observation has ever been used as a contribution in legal cases, for example to speculate on the time of the last meal. I am examining a dog killed by a gunshot wound: the stomach is full of food only partially digested, but the gallbladder is not empty (the bile duct was normally patent). Can I infer something about the time elapsed since eating its meal?
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Starvation/fasting may lead empty gallbladder.
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every thing about cats and dogs psychology in mating
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You should learn all about evolution of reproductive systems with their mode of action and finally zoopsychology.
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I have heard it is very important when do animal experiments, particularly in dogs, to select a internationally recognized provider as Marshall. Apparently there are small differences on the homegeneity of the genetics of the breeding despite they are all beagles.
Seems that Marshall are too expensive and therefore CROs using these animals despite credible and quality are more expensive than others as for example CROs price diffeerence between Europe or India as example
So then question is: any of you have experience on that? Is that much the difference? Do you recommend not to use beagles that has not sufficient credibility regarding beagle breeding homogeneity? Are AAALC accreditations suficient to guarantee he homogeneity of the breeding? Is there any specific ethical rule or regulatory rule that I should take into account for generation of the data?
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On homogeneity they may have duplication of genes. Genetics are more complicated but very interesting.
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Well, I think that animals and us (animals too) are both intelligent and rational, we just evolved, back in time we were "dumb" as we think animals are but who thinks this way is wrong, you don't see animals throwing themselves from edges, when a dog goes to a precipice they don't jump to death, they have the ability to rationalize situations, dogs can cross streets, help blind humans to walk through cities and even find cancer presence only by smell, and many other things that we can't. In fact, what I think that differs us from them is the dexterity achieved by humankind, the know-how acquired by us is on another level, we were able to think, to question and to answer that questions, what really differs us is our philosophy and science, our mind and brain evolved too much and got the capacity of creation.
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In short, I don’t think so because I don’t believe in Darwinian theory.
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Came across a case of subcutaneous swelling located between the xiphisternum and umbilicus in a 3-month old male German shepherd. According to the animal owner, the swelling was since birth and growing progressively. General health condition of the animal was otherwise healthy. Palpation revealed a reducible mass with 2-fingure defect immediately caudal to the xiphisternum directly over the linea alba. Sonography & abdominal radiographs confirmed herniation of the stomach and associated viscera. Please suggest an exact terminology for this defect.
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Vineet,
I'm not a vet, so I don't know the exact term. As a linguist I'd suggest using "Canis linea alba inflammosis" (Translation: Swelling by Linea Alba Dog Disease).
Regards
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Covid-19 is said to have originated by mutation in a 'wet market' in China. These may contain a wide range of live or dead animals, including dogs and cats. Does anyone know what animals, wild, feral or farmed, have been implicated as possible sources?
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Hi,
I am doing a GWAS analysis of european bison's a dog's SNPs. Right now I am doing multiple data clean up, according to previous studies and what I have learned.
I was thinking, if it is ok to test HWE and discard data that does not fit if I am working with animals, where mating is controlled and inbreeding coefficient is high?
And what about tests for population structure etc.? It is obvious that there is going to be some structure in reintroduced and domestic animals. I don't want to loose any important data and I want to be sure, that I have done everything correctly.
Thank you for your help.
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Lenka Ungrová If your population is large enough, it is recommended to perform HWE test.
I feel these papers give you some insight into which decision might be right for you:
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I need to provide the number of haplotypes in my data for the hapFLK test (Fariello et al. 2013, Genetics).
I try to find the number of haplotypes in my whole-genome dog and wolf data (21Mb SNPs) with fastPHASE cross-validation procedure.
I created a test file (attached) and ran fastPHASE with different settings including:
./fastPHASE -T10 -KL3 -KU30 -Ki2 -Ks50 -Km1000 test.PHASE
However, fastPHASE always outputs K15, which I believe is incorrect because it never changes even if I change the input file. It also never outputs a file with _kselect extension, as it should. Maybe there is a bug. I use fastPHASE 1.4.8, it is available here http://scheet.org/software.html I tried to contact the author of the software, but have not got a response.
I also tried to use PHASE program, but it is extremely slow and cannot handle even a subset of 7K SNPs.
Could anyone share his/her experience with estimation of the number of haplotypes with fastPHASE or any other program?
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According to the program developer, fastPHASE 1.4 doesn't support any more a cross-validation option. That is why I created a solution to estimate the optimal number of haplotype clusters for hapFLK with fastPHASE 1.4. It is packed as an R package imputeqc -> https://github.com/inzilico/imputeqc
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Just this week, I noted two cases of extreme mange in coyotes in my research area. Coincidentally, I had camped out by a cattle trough in the area overnight in September and three days later both my dog and I had a bad case of Scabies, (same thing as mange).
Has anyone dealt with this in wildlife species? What organization do I contact with my findings?
I'd appreciate advice.
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Please take a look at the following PDF attachments.
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None of the controls I use for rat, mouse or human/monkey work.
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Hi Jose, did you ever find an answer to this question? I'm looking for one too
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Hello
I have a some draft for a title questions ...
THIS DOG SAVED MY LIFE: To What Extent Does Formal Canine Therapy Intervention Impact on the Healing and Reintegration of Survivors of Conflict Trauma?
THIS DOG SAVED MY LIFE: What are the Merits of Formal Canine Therapy Intervention for Survivors of Conflict Trauma as Opposed to Informal Interaction?
THIS DOG SAVED MY LIFE: Does Canine Therapy Intervention Offer a Sustainable and Cost Effective Method for Aiding the Healing and Reintegration of Survivors of Conflict Trauma?
Any thoughts?
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I like 3rd but maybe too long, and then 1st
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I have a dog who loves cats, he is a puppy and from 3 to 9 months he has been exposed to a few cats. He mostly chases them benignly underneath things and then cries to play with them. He has been able to put his nose on the friendliest indoor cat when he is lounging. Where does this go next?
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While there are some parings that work out in days, in some rare cases, it never works out. Liz Palika noted that, in her experience, the "get acquainted" process usually takes two to three weeks. Dr. Landsberg noted that sometimes it's not easy to tell by their interactions if a dog and cat are getting along or not.
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I am gathering bibliography around home range measurement with the traditional method, alias observation.
  • Do you have studies to suggest?
  • In particular, are you aware of recent publications, say within the last 15 years, still using observation?
  • Do you think this can still be a valuable way to measure home range size of some species, as, free-ranging dogs living around a neighbour?
Thanks!
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Thank you for your valuable opinion and suggestions. I will keep those in mind for considerations and future actions.
Your example of attaching a GPS to dogs would have been ideal, but that was not doable for several reasons. Though, preliminary and specific observations suggesting that dogs have a recurring home range pattern has allowed to gather data by empirical observations.
If you are curious to check at previous empirical data collections, please check infographics by this link:
Would you have any other consideration, idea or suggestion,
I'll be more than glad to hearing from you!
Thank you and Best Wishes.
Marco
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Sci Am October 2019 issue has an article, Is Death Reversible? by Christof Koch of the Allen Institute for Brain Science. At p. 36 he writes; `... there is no credible evidence that apes, dogs, crows and bees have minds sufficiently self-aware to be troubled by the insight that one day they will be no more.’
Relate this to a preceding question: Is abstraction a collective invention of human society? Suppose that abstraction is an invention of society’s networked minds of hundreds or thousands of generations. Then the difference between `apes, dogs, crows and bees ‘ and humans is that humans acquire the abstraction `death’ from the store of abstractions devised collectively by society. If so, humans do not differ from `apes, dogs, crows and bees ‘ in understanding death due to superior individual cognition that arises during an individual lifespan, but rather from an insight collectively arrived at by networked minds over many generations.
For an individual human, it is difficult to separate from knowledge that which has been acquired by means of language. Paul Feyerabend in 1993 in Against Method, observed ` … describing a familiar situation is, for the speaker, and event in which statement and phenomenon are firmly glued together.’ Perhaps that applies to the abstraction `death’? Or not?
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I don't see how else a person could grasp death other than abstractly. To know death experientially (as-lived, real-time, as-experienced) one would have to die, which would be rather difficult to tell about it. Perhaps being really close is good enough but still it is not death. Curiously Heidegger suggest confronting our personal death as a way to allow our personal concerns (our throwness to seek wealth, fame, mansions, yachts, PhD's or whatever one has be the golden ring) to negate these yearnings' influence on our 'being-there' (dasein). Then we can see without these 'fallen' ways of being clouding our perception. Would this be akin to a personal Plato's cave experiment, whereby one sees the world not the shadows on the wall?
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I have changed my extraction method, changed my PCR master mix, changed my water. also I have modified my PCR program but nothing works. and when I got a single band and sent it for sequencing they didn't give me a clear, reliable read.
PS. My primers are ITS1F and ITS2R4.
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Dr Narmin, In addition to Bhairavi's reply I would also suggest you to optimize the quantity of your template too. Often higher amounts of template generate non-specifics. Next, for clinical samples or samples that are expected to have other organisms too, even if you see a single band in gels, it might contain identical/similar length amplicons from other species/ non specific targets. So you cannot expect a good sequence read from direct sequencing of such amplicons. You have to do a clonal-sequence analyses. best wishes, SD
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In India about 20000 people per year die due to rabies and there is an urgent need to control this disease.
The primary source of infection is stray dogs and there is an urgent need for vaccination of stray dogs with rabies vaccine for effective immunity is for 3 years.
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Please go through this useful RG link.
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Looking at the similarities that dogs and babies share. How do reward and treat behavour controlling cross from one to another ? How do these practices feed into normative ideas of success and failure ? Do practices of 'petification' (halberstam) aid the adult/owner or the child/pet ?
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Do not agree with this principle, the child should be treated as a dog because the child is a human being and differs from the animal
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I am thinking of creating a search engine to help people find a movie or similar movie based on the snippets of the story. For instance, if a user type in "movie about dog waiting a long time for his owners to come back", the result should return "Hachiko" , "Eight below", "Lassie" etc. However, it would have been better if we can use data mining method to actually search it based on the plot of the movie not keywords. ​What is the best solution for this work?
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@Raju_Balakrishnan3
Thank you so much.
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There are many health benefits of sharing your life with a dog. I made a summary here
But are there any good evidence based scientific studies?
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Dear All,
I performed a compound PK analysis in Dog and Monkey. The PK modeling is based on non-compartment analysis (NCA) and, I found that the compound's t-half time much longer than its mean residence time (MRT).
I was wiondering if anyone also observed this phenomena before and could please give me any suggestion to explain the data.
Thank you so much!
Best,
Tianzhu
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Hello Tianzhu Zang,
You did not mention how the drug was administered. If the drug was administered IV and follows one-compartment, then MRT > t1/2. If two compartment occurs there a strong possibility that MRT < t1/2. Several years ago two authors, Sobol E, and Bialer M published a paper pertaining to an equation that is applied to this phenomenon. You can access this paper as follows:
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I found that it takes 4-5 months for the dogs jaw bone to become completely decalcified , but this is a long period.
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I suppose you use EDTA. It is slow, even for mouse bone it takes 4-7 days. Regular solution replacement will speed up process, but not drastically. And what tissues are you talking about? Big specimens are nearly impossible to fix properly, and after 4 months without fixation tissues will look not good. May be you can cut slices of specimen from the places of interest? If it is a collagen you are interested in, it is almost non destructible and you can use stronger acids, like 30% phosphoric or 5% nitric.
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based on observation of my (young) dog I'm starting to think that thay are capable of conceptual metaphoric thought.
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Hi! Take a look at this:
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Hi guys,
I'm having problem growing bone marrow MSCs isolated from dog. I froze the cells at P1 and recently started to revive them. All four batches of cells (from 4 animals) are growing okay in T25 flasks at P2. But once I passage them and grow them in a larger flask T175, 2 out of the 4 batches do not attach or deattach from the plate, and there are cell debris in the medium. The 2 batches of cells that deattached were isolated from younger dogs than the other two. But only 5 days younger.
Any one have a clue on why this is happening? Should I keep use T25 four further passages instead of transfering them to large flask?
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At first I used 1 min of TRP 0.25% but I noticed sometimes cells didn’t grew well. With 5 min of 0.05% cell grow much better.
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I am a civil servant working in the special department related to stray dogs. As the conductor of the description of the mutual assessment to get dog-food supplies, I have to be accurate about the reason I need to ask for dog-food protein rate around 18-22% with the detail of it containing not less than 18% biologically originated protein. Can you please inform me about the proper legislation referring it? A thousand thank you in advance.
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Thank you so much for your immediate answer
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For a project I need DNA samples from different organisms (cat, dog, horse, plants etc).
Do any of you know of companies that sell DNA from different organisms?
Many thanks in advance!
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For a project I need DNA samples from different organisms (cat, dog, horse, plants etc).
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Hello all,
I came across two deformed Rhipicephalus sanguineus fed females, please see attached photos. Female 2 weighs 0.265grams and the weirdest one, female 1, 0.209g. These were just slightly lighter weights than normal females. Have you ever seen this? It does not look like gynandromorphism to me. Maybe dog bite trauma?! 
They started incubation to lay eggs and 3 days later they started oviposition. Eggs are healthy looking. I can share the specimens and the progeny if any interest. I can't take time to dive into this, but I thought that someone here might be interested.
all the best,
Flavia
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I agree with Dr. Gruntmeir. I've seen this and others tick teratology before that can occur due to excessive inbreeding within an old tick colony. Introduce new wild specimens in your tick colony.
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Hello
I am planning to insert a mouse gene into dog genome via CRISPR cas9 to re-activate cell proliferation pathway. Not sure where to begin. If anyone who has done a successful CRISPR Cas9 experiment before, can you please share your experience with me e.g. how to set up the experiment? any help would be highly appreciated.
Many Thanks
Regards
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Kavita, Yes I believe HDR template is necessary along with sgRNA.