Disinfectants - Science topic
Substances used on inanimate objects that destroy harmful microorganisms or inhibit their activity. Disinfectants are classed as complete, destroying SPORES as well as vegetative forms of microorganisms, or incomplete, destroying only vegetative forms of the organisms. They are distinguished from ANTISEPTICS, which are local anti-infective agents used on humans and other animals. (From Hawley's Condensed Chemical Dictionary, 11th ed)
Questions related to Disinfectants
Ethanol prices have tripled since 2020 making the use of 70% ethanol non viable. I am looking to buy bulk quantity of disinfectant for our new laminar flowhood.
I am wondering which type of disinfectant are you using in your laminar flowhood and why?
Based on current research I am convinced that quaternary ammonium disinfectants are the most effective and economical and I am leaning towards them but I would like the opinion of other fellow microbiologists.
I'm looking for papers and articles regarding the post-evaporation residue of healthcare disinfectants (based on CDC guideline: link below) like H2O2 or sodium hypochlorite. I'm also looking for the association of supposed residue to biofouling and biofilm formation.
I've read that H2O2 doesn't leave residue but they are mostly anecdotal.
Chlorhexidine (Chlorhexidine gluconate, digluconate or chlorhexidine acetate) is an antiseptic that is used in medicine for last 70 years. Is there any experience of Chlorhexidine application to control greenhouse, water, soil contamination by plant pathogens?
In fear of corona disease, the whole World has been used many disinfectants and chemicals. Thus it needs to think about their bioaccumulation effect and suitable remedy measure simultaneously to save the Earth from any type of environmental problem in the near future.
I am trying to perform statistical analysis of my data, which is OD of bacterial cells in serial dilutions of three different disinfectants. I am facing a problem where my stats don't match the bar charts on the graph, e.g. visually there is significant difference between the treatments, however my p value is way above the 0.05 threshold. I performed multiple unpaired t tests to compare the treatments with each other, as well as two-way ANOVA for the overall comparison. Could anyone please explain if I am missing something in my analysis or perhaps doing it wrong?
I've conducted crystal violet biofilm assay, testing iodine and chlorhexidine based disinfectants against E. coli (NCTC 11560) and S. aureus (ATCC 29213). With the iodine disinfectants, I got an expected pattern, where less biofilm is formed at the highest concentration of disinfectant and more is formed at the lowest concentration. However, in the case of chlorhexidine containing disinfectants, the pattern appears to be in reverse, with relatively strong biofilm formation at the highest concentration of the disinfectant, and the lowest growth at the lowest concentration.
The picture attached shows a two-fold dilutions of two iodine disinfectants (columns labelled GG and LD) and two chlorhexidine disinfectants (columns CD and SC) tested against E. coli biofilm. Has anyone witnessed similar patterns before or can suggest any literature explaining it?
I am planning an experiment to check the combination effect of 10 different chemicals for cytotoxicity. I know their individual effects and I would like to check their interaction effect or combination effect on cytotoxicity levels. Some compounds are pure biocides and some have low to moderate cytotoxicity. I came across fractional factorial design of experiments. I am not experienced with statistics so it is quite overwhelming to go through all the basics and calculations. What would be the most convenient way to carry out such an experiment? An expert's advice and help would be much appreciated.
In all labs I saw, I found that ethanol is used for sterilization of hands etc. in experiments with microorganisms. Why is isopropyl alcohol not used while it is relatively cheaper? Is it harmful for skin upon frequent use?
Databases of antimicrobial resistance (AMR) genes have been well established, but I wonder is there any similar database of disinfectant resistance genes. Does anybody have any idea about this?
Our lab is hoping to (safely) start human subjects research again. Would anyone happen to know the best procedures to disinfect velcro skin conductance electrodes? We are concerned as to the impact of either water or chemical disinfectants on the equipment.
Phenol toxicity is reported in cats, especially related to disinfectants and essential oils but a toxic dose is not usually indicated. Can its use be toxic to feline patients that need repeated administrations of a medication containing phenol as a preservative?
Are chlorin compound and quaternary ammonium compound as a disinfectants kill the COVID-19 coronavirus?
The disinfectant chemicals can get into sewage systems and pollute drinking water resources. Chlorine disinfectants threaten aquatic plants and wildlife by destroying their cell walls or damaging their proteins by oxidation. The disinfectant chemicals can bond with other materials to form harmful compounds. In addition, disinfectants could combine with nitrogen, forming chloramine or N nitrosodimethylamine, which have been identified as carcinogens.
I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.
I am working on an experimental SWRO plant with Ultrafiltration module. The Chemical Enhanced Backwash cycle for the UF membrane constitutes a biocidal flushing (NaOCl) followed by low pH HCl (2-3) and finally high pH NaOH (11-12)wash. The backwash solution is generated by injecting the said chemicals directly into UF permeate line from UF permeate storage tank. It is observed that that the NaOCl and NaOH dosing ports are getting frequently clogged due to precipitation of calcium carbonate (confirmed by chemical analysis of the precipitated scale).
Any suggestions on how to prevent this without reducing the pH (since the high pH is required for efficient cleaning)?
Can some alternate dosing arrangement be made to prevent this? Or using RO permeate instead of UF permeate solve this issue? Any other suggestions based on experience are also welcome.
we'd like to clean and decontaminate our liquid nitrogen tank before stroreing new cells inside.
The tank seems to be made of aluminum. Any recommendations on what disinfectant to use and for how long it should incubate?
Stay healthy and safe!
Don't you think we ought to add this to our preventive measures awareness:
Reusable Face Masks Should be washed with disinfectant or good quality detergent and well dried to keep it in safe conditions for reuse. We should remember this.
The disposable Face masks are used and safely discarded.
Our Health singles on media should educate people, especially those who may not know.
It's a tough battle against Covid-19.
We are all in this together.
Stay safe, play safe..
Plse, your views and contributions on this
I need to do it only in microplates and read the OD value for the results. I cannot use any other assays except microplate. Please help me with this
Antibiotic resistance and the emergence of many new viral strains due to climate change, animal farming and deforestation are increasing the usefulness of pharmaceutical agents, incl. antibiotics. We are currently dealing with yet another viral pandemic. Are there any natural plant extracts that are being studied that are effective disinfectants for the whole suite of fungal, viral and bacterial infections that act as pathogens to humans ? Particularly interested in those effective against coronaviridae ?
70% ethanol or Isopropanol is commonly used disinfectant in cell culture lab. Apart from that what is the best fungal disinfectant for mammalian cell culture. is H2O2 effective against fungus??
I understand that the toxicological effects of the two are different.
Sodium Hypochlorite seems to be far more caustic, toxic, and irritating in general than Hypochlorous Acid; there is much toxicological information and evidence that suggests how the hypochlorite is much more irritating than hypochlorous acid.
However, at the chemical level, Hypochlorous Acid is a strong oxidizer and can penetrate through cell walls of pathogens much more effectively than Sodium Hypochlorite because of its electric neutrality. Since that is the case, wouldn't it be more dangerous to mammal or human cells as well?
Vitamin C seems to be the only avenue for protection against the oxidating power of the Hypochlorous Acid that seems to denature proteins and engage premature cell apoptosis.
I continue to find the concern that chlorine gas is released from sodium hypochlorite; however, that only happens when the hypochlorite anion is acidified to Hypochlorous acid, and only then does it dissociate into Chlorine Gas.
If Hypochlorite were to be the direct source of chlorine gas, what would be the specific mechanism from the Sodium Hypochlorite to the formation of chlorine gas?
Dear fellow researchers
Is it possible to use SDS as a disinfectant for when you want to put equipment into the laminar flow cabinet ( for when you want to do cell culture work)?
I already know solvents like NMP, HMPA, DMF. They are highly toxic to work with and not suitable in Food, drug, pesticide or biocidal applications.
Hi everybody: i am looking for recent datas about the amount of registered biocides containing silver nanoparticles. The most recent data i found is in this paper from Nowack et al. (2011) which explains that about the 53% of biocides registered at EPA contains nanoAg and the 7% of it contains AgNPs. I looked for more recent datas/numbers about biocides cointaining nanoAg/AgNPs, but from main sites like EPA's, REACH's and ECHA's or from the huge amount of papers i read nothing came out. I was wondering if someone has something (papers, reviews, sites etc..) to share with me to help my research. Everything is accepted but it must be referred to biocides containing nanoAg/AgNPs.
Thanks a lot to everyone who will share his/her time with me
Microbiomes are affected by disinfectants and sanitizers. Excessive use can reduce the density of 'friendly microbes' in biotic and abiotic ecosystems. The dilemma is: in fighting against one, we might be losing several that defend! Because of the complexity of the situation, do our tools allow us to conduct studies on these aspects in order to forecast or predict about the newer threats?
Disinfectant chambers do not get rid if viruses inside your body. But it can do a lot of harm. They serve no purpose but are harmful ?
With the COVID-19 virus situation, many individuals and organizations are developing various protective equipment to control the spread of COVID-19 as a every nations. The sterilization chamber is one of the most popular defenses.
The main issue in this sterilization chamber is whether sterilization is effective ? . It has not been scientifically proven that efficiently disinfecting the body of the virus by spraying an antiseptic in a closed chamber ?
Although surfactant disinfectants are generally considered to have virucidal activity against all lipid enveloped viruses, some authors claim their inefficiency against SARS-CoV-2 (as it happens for non-enveloped viruses). Despite its importance, this question remains unanswered over time. I would like to exchange scientific opinions and evidence on this important topic.
All products on this list meet EPA’s criteria for use against SARS-CoV-2, the virus that causes COVID-19.
List N: Products with Emerging Viral Pathogens AND Human Coronavirus claims for use against SARS-CoV-2
I am inviting researchers for collaboration on a research project, which includes the testing of some nanotechnology based disinfectants (surface disinfectants) efficacy testing for different microbes and viruses. I will try to provide concerned developed product samples for different experimental procedures.
Should the tanks be configured in series or in parallel?
Good operation of a water supply network requires two or dual water storage tanks. This allows the utility to take one tank offline for maintenance and still being able to maintain supply to customers.
On the other hand increased hydraulic or retention time will lead to loss of disinfectant residual and production of disinfection by-products.
At the same time the utility needs to maintain complete mixed conditions in the tanks.
Which configuration will have the lessor retention time?
Infection is a huge problem in hospitals, especially as they get larger and infections become resistant to antibiotics and disinfectants.
Does anyone know of new IC models or IC risks?
One of the methods we use for IC is disposable gowns and gloves - but when does the disposal of these become a risks in themselves? When does swabbing floors and surfaces spread infection, rather than prevent it?
Our team is looking for viral RNA in the environment. We took swabs off of doorknobs using cotton dipped in water and then submerging that swab in viral transport media. We also wiped 1 surface with disinfectant spray thoroughly as a negative control and took a swab of that. We did RNA extraction of 6 environmental samples (including negative control), and 5 samples of our model virus (Eliat) in our transport media, as well as in water, with and without our cotton swab.
Every single sample showed a decent curve on the nanodrop at 260 after the RNA extraction.
Every sample showed a concentration of around 80 nanograms/microliter.
Every sample showed a 260/280 of around 3.40
Every sample showed a 260/230 of 0.17 - 1.02
It seems so strange to me to see these results be so perfect.
Could this be contamination in all my samples?
Could the kit I used be saturated in RNA which is why I found the same concentration?
I'll double check the disinfectant I used but is it possible that it didn't destroy RNA on the surface I applied it to?
What I'm going to proceed doing:
I have primers for Covid (what we were testing the environment for) as well as our model virus however the model virus has no control for PCR. I plan on running an RT-PCR tomorrow but I highly doubt I'll find covid. My model virus primers have yet to be used so I'm curious if they even work. If they don't work I have no way to verify if my RNA extraction was successful or not.
What disinfectants are recommended to be used to clean the environment in health care facilities or homes where patients are suspected of being infected with the emerging coronavirus?
In Table 1 of an article, Persistence of coronaviruses on inanimate surfaces and their inactivation with biocidal agents, by G. Kampf, D. Todt, S. Pfaender, E. Steinmann that appeared online February 6, 2020 on the website for Journal of Hospital Infection are set out persistence of coronaviruses on different types of surfaces.
Does the concept of half-life apply to an aggregation of corona viruses? Are there any such studies?
It would seem unlikely that all the virus particles become inactive at the same time. Do they?
I've been asked to formulate a disinfecting solution that can be used in walk through booths for people entering our facility. I've searched the internet but sources are pretty vague:
1. In China they refer to it as a hospital grade disinfectant
2. In Jakarta they refer to it as similar to hand sanitizers
3. In Vietnam they say its an anolyte solution using sodium chloride.
4. Others suggest it is chlorine based.
Any insight to this would be greatly appreciated!
As mask are used frequently by common people,so it would be better to use cloth mask and can be washed with detergent ,so that it would be disinfectant and reused also. So that problem of disposal remains minimum.Also medical staff used ppe should be disinfectant properly.Also where patient has put, waste of them PPE,water used etc should be disinfectant properly to avoid any chances of spreading.
Looking for a material that is active against bacteria and viruses i.e. it is anti-bacterial and anti-viral. Also when applied to a plastic film stable with anti-bacterial and anti-viral for 2-3 months. Thus it is self dis-infecting and when coated on plastic film it will help prevent infection and transmission of infecting agent.
For effective antiseptics to eliminate the virus, tests conducted on different disinfection solutions have shown that antiseptics contain compounds “ethanol” (a concentration of 6-71%) or “hydrogen peroxide” (a concentration of 0.5%) or “sodium hypochlorite” (concentration 0.1%) effective against corona viruses.
According to various studies, if infected contaminated surfaces and areas are cleared with appropriate concentrations of these disinfectants, they reduce the number of infectious coronaviruses from one million pathogen particles to only 100 within one minute.
On the other hand, tests showed that there are other disinfecting solutions that have been shown to be less effective in controlling Corona infection, namely benzalkonium chloride (0.05-0.2% concentration) and chlorhexidine de gluconate (0.02% concentration).
Corona is a novelty in the family of viruses that have an oily membrane and is strongly affected by disinfectants, so it is very easy to eliminate them with disinfectants, such as water, soap, alcohol, ethanol, oxygen water and other disinfectants, as disinfectants of any type affect this virus and reduce its focus on surfaces, but It is preferable to be in suitable concentrations, in order to completely eliminate the virus
I've been using 1:10 to 1:100 bleach solution, but my PI has concerns of the corrosive nature/safety of bleach. I've been trying to find an SOP for cleaning lab floors; does anyone have suggestions for disinfectants and SOP for cleaning lab floors?
We are evaluating the effectiveness of disinfectant and we need this document (link below).
ASTM-E1054 › Standard Test Methods for Evaluation of Inactivators of Antimicrobial Agents
Could you please show me how to download this document?
Thanks and Best regards.
Does anyone have any information about tribromamine such as Henrys law constants/air measurements? In ECHAs Guidance on the Biocidal Products Regulation (https://echa.europa.eu/documents/10162/23036412/bpr_guidance_vol_v_dbp_new_en.pdf/c7d11d09-8ae5-317f-0eeb-ec8b2aa938b3), it says that no data are available for tribromamine but by extension this DBP may also be considered relevant for air. I wonder if this component can be assumed equally volatile as trichloramine?
What are the main componens/microorganisms causing skin irritations in swimming pool waters?
I want to insert a metal probe into the body. This probe is a metal enclosure that contain of an optical fiber. The light comes out of the fiber end and through the hole in the end of the tube. I want to paste the optical fiber to the metal enclosure with a transparent biocompatible adhesive. What is the best adhesive for this work? This adhesive should to be resistant to disinfectants agents.
I'm doing a research about tissue culture and I think I don't have enough knowledge about it. What are the problems that I will face on my research and what are the solutions? What part of the plant will I use? Will I use MS medium or other mediums? What disinfectant will I use? I hope you can help me and thank you in advance.
When the drinking water is treated in a water treatment plant, it is dosed with chlorine in order to kill bacteria and keep the water safe in the distribution network. This water is then supplied to our households in two ways: one is directly through the pipelines and the other is that it is sent to a distribution station/booster station or reservoirs, where it is dosed again with chlorine or another disinfecting agent and then pumped to our household through pipelines. The second method is known as post-treatment chlorination. I would like to know what are the various disinfectants used for the re-dosing at the distribution station, in what state, quantity and at what point are the dosed in the distribution network. All inputs are welcomed and thank you for your time.
I am not sure as to what chemicals I need to add to increase the viscosity and still get the same disinfection results or better.
We have previously used Aquaguard-2 (Biological Industries Model: 01-916-1E), but this product has been discontinued. Therefore, I am wondering if anybody knows of a similar product to be used to disinfect water baths.
Many disinfectants and other methods when used in the field, (such as UV), rather than in a lab environment, have been shown to not be highly effective at addressing the presence of dry surface biofilms.
For example, do isopropanol/1-propanol/ethanol alcohol based hand rubs all use the same mechanisms to kill bacteria?
Do they all just disorganize lipid membranes, denature proteins, and coagulate proteins?
Since i am working with the disinfectants to check its antimicrobial activity , my standard says different types of neutralizer agents , but the common percentage of neutralizer or the quenching agent to stop the antimicrobial activity of disinfectants , cant able to determine .
I understand that disinfection is to eliminate most harmful microorganisms from objects; while sterilization is to to kill ALL microbes - whether harmful or not.
I wonder how can a certain disinfectant (for example chlorine ) be selective and distinguish between pathogenic and non pathogenic microorganisms?
In other words, what is the mechanism that exclude non harmful microbes in disinfection process?
thanks in advance
Please give me details about efflux roles in resistance against biocides. How efflux could perform their functions against biocides?
We all know that bacteria became resistant to antibiotic with frequent use of antibiotics, my question, Why bacteria can not become resistant to disinfectants in the same mechanism of antibiotics resistances?
what is the difference between Trihalomethane Formation Potential and Simulated Distribution System Trihalomethanes to study the removal efficiency of disinfection by products precursors in artificial water samples
We were using Hydrogen peroxide as disinfectant. But it was nor effective all the time. How can we improve the fungicidal activity of hydrogen peroxide and what are the other product which can be use as fungicide in water system.
EPA CT was developed to assess the performance of disinfection systems mostly related to drinking water biological quality. EPA CT Tables provide CT values for the major drinking water disinfectants such as: chlorine (free chlorine), chloramines, ozone, and chlorine dioxide. The CT for chloramines was developed based on disinfection data collected from preformed chloramine (which means chloramine solution was prepared and the chloramines were already formed in the solution before being used for the disinfection). However, in the field, current chloramine application uses the sequential method where free chlorine is applied first for certain period and then ammonia is introduced in the system to produce the chloramines. This method of chloramine production is different from the method that EPA used (preformed method of chloramine production) to prepare the CT tables. MIOX (a mixed oxidant system) operates a mixed oxidant solution including free chlorine, hydrogen peroxide, and others that are simultaneously produced in the disinfection solution. MIOX uses EPA CT tables to assess its disinfection performance, although the disinfectants are simultaneously produced in their systems. Here multiple disinfectants are systematically formed in the process, while EPA CT tables are based on single disinfectant systems. From the above two cases, it can be observed that the method of disinfectant production seems to be irrelevant to the use of EPA CT tables.
I am performing growth curves analysis using a Bioscreen C Analyzer and honeycomb microtitre plates. I am exposing 2 species of enterobacteria to two-fold serial dilutions of a particular disinfectant. In my blank wells I have everything but the bacteria. The machine reads the OD600nm every 4min during 24 hours, with continuous shaking between the measurements (incubation in the dark at 37 oC). The machine can accomodate 2 plates, and on each plate I have one column (10 wells) dedicated to the blanks, each well of this column contining a different concentration of my disinfectant.
So far I was calculating the average between my 2 blanks for each time point and each concentration, and then removing it from my sample value. I then take the ln of this value to plot it against time and calculate the slope during exponential phase to obain the growth rate. However, it almost always ended up with my blank OD being higher than my sample OD (containing disinfectant and bacteria), and it is not possible to calculate ln of negative values. I miss the exponential phase and can't properly calculate my growth rate on my curves as I miss too many data to properly evaluate it. I tried to blank with the minimum value of my 2 blanks but still observe the same result. Moreover, I observed a huge variability of my blank OD for each well over time (due to the temperature?), between my 2 blanks in the same experiment, and between my blanks from different experiments. I understand that I can't ignore my blanks and just use my sample OD as then variation in my sample OD would be partially explained by variation in my blank OD (directly linked to my compound I guess).
Does anybody have an explanation for this and, maybe, a solution to my problem? Do you have encountered the same kind of issue before?
Thank you for your help,
Biofilm formation on medical devices is one of the important problems now due to difficulty of its removal and the complicated microbial infections as well as the decreasing of the effective of antibiotics and disinfectants.
Hello, I am struggling with a question on how to come up with the most suitable disinfectant, soap, or detergent for cleaning my plastic 30 L tanks, used to collect tap drinking water samples.
Currently I am using Chlorine containing tablets that turned out to be not very efficient because the solvent cannot be removed completely even after several washings, killing afterwards all bacteria in the sample, giving a poor DNA concentration results.
The is one option that same to our mind: it is hydrogen peroxide, but it would be nice to have some more options.
Could you please suggest some easily removable but efficient soaps, disinfectants or detergents with short contact time.
To have an overview, I am doing the following:
1) Colecting Demi water to the tank, adding the tablet
2) Waiting for about 10 minutes until it is completely dissolved
3) Mixing and shaking the canisters
4) Pouring water with the detergent
5) Washing the canisters for about 3-4 times with fresh demi water until the chlorine odour is gone
Does anybody know something about spores of Enterocytozoon Hepatopenaei especially with regards to its stability against chemical disinfectants and UV light?
I would be appreciate for any information/links/articles or thoughts about this issue.
I have already found that it is quite new organism and there is not much researches dedicated to this problem.
Present water supply treatment uses chlorine as primary disinfectant followed by the addition of ammonia to form chloramine as a secondary disinfection. When water temperature is above 23 -24 degrees centigrade nitrification is severe in downstream storage tanks. Decay of 1 -1.5 mg/L total chlorine is also observed in the bulk water mains before reaching the storage tanks. Treated water leaves the water treatment plant at pH 7.5 -7.8. Although not widely reported some water authorities had demonstrated that chloramine is much more stable at pH 8.5 - 9.0.
How we can overcome to internal bacterial infection in blueberry for in Vitro healthy plant establishment? We used different antibiotics and disinfectant agents like NaOCl and Hgcl2, but don't achieved interested results.
hi all. currently i have problem regarding harvesting high titre of BVDV. normally i just manage to get logTCID50/mL 5.5 which is very low as i have to get at least 8.0 for disinfectant virucidal efficacy. i try to infect with low MOI, but also not achieved. does anyone here used to work with this virus and how to optimize the infection so that i can get high titre? help.. its urgent. thanks
I'm currently working with a small flow-trough UV-LED reactor and I'm testing it to find how well it disinfects water. Right now I'm just preparing a "contaminated" solution, passing trough the reactor y measuring the disinfection rate.
I would like to improve my calculations with knowing the exact dose that the reactor is giving to the contaminated solution. I know I could use a photometer to measure the intensity of the light and calculate dose, but due to the reactor design, use the photometer would be very complicated.
I would like to find any solution/material with a very well known reaction to UV light in order to correlate that reactor with a dose.
I hope I explain myself well.
Thanks for your help and ideas
I have recently tested the antimicrobial effect of disinfectant on E. coli. Paper disk assays were soaked in disinfectant and transferred on E. coli agar plates. Clearly, inhibition of bacterial growth has occurred but there seems to be a blurry halo around every disinfectant disk (20 to be exact). I'd appreciate your input!
I am attaching a photo
5% hydrogen peroxide solution with the addition of 0.2% silver nitrate as a stabiliser is a commonly used fogging disinfectant. However under new regulations, silver nitrate is banned for fogging on account of its toxicity. Some manufactureres of these solutions have now altered their MSDS to show metallic silver rather than silver nitrate as the second component. I have a suspicion that this is misleading, as metallic silver, particularly in a finely divided state, should break down the H2O2 in to water and oxygen.Can anyone confirm or refute this theory?
My institute uses Steritech CAS for everything. Besides quats, it also contains a tin compound which to my understanding is really bad for aquatic organisms. We use lots and lots of it because we are a TB research institute. Does anybody else know if other high level disinfectants e.g. Virkon may kill TB and are less damaging to the environment?