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I've been interested in COVID stuff recently. Most of the current detection/diagnosis strategies need amplification. I can understand that RT-PCR can probably break the capsid because of the high-temperature processes. But how does that work for isothermal methods like RT-LAMP, since the temperature is not that high?
Also, if we want to directly detect or do something with the viral RNA, do we need to break the capsid and release the RNA? If yes, is there any established protocol? Many thanks!
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Heat treatment of swab samples for 5 min at 95°C before RT-LAMP (hot swab–to–RT-LAMP assay) shows good results.
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Hi,
I am developing a research protocol to test the inter-rater reliability of a measurement tool for learning. I see that the minimum acceptable kappa value could differ from discipline to discipline depending on the degree of inter-rater agreement required? for instance, a higher degree might be needed in medicine/disease diagnosis than in education?
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Check for the work of Lawshe 1975
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We are working on heart disease diagnosis and for this purpose we will required a database so it could be useful for us to develop an expert system.
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I have a special issue entitled "Deep Learning for Chronic Disease Diagnosis, Prediction, Monitoring, and Treatment."
The issue has the following link:
You are welcome to submit your research papers in this issue. Please note that the Diagnostics
Journal has 3.110 impact factor.
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Special Issue "Advances in Deep Learning Ⅱ"
Print Special Issue Flyer
Special Issue Editors
Special Issue Information
Keywords
Published Papers
A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Computing and Artificial Intelligence".
Regards,
Shafagat
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The research question is: Does gender influence stress levels four month post-diagnosis of cancer?
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Very interesting information
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Is it feasible to replace an ophthalmologist by AI to diagnose the main eye diseases -diabetic retinopathy, glaucoma, age-related macular disease ?
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Assist - definitely yes.
Replace - not yet.
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Hello everyone! I am currently doing an analysis of the occlusal analysis. Now I have a pressure sensor for the mouth, just like Tek-scan. I am trying to link stress analysis with a certain oral disease (such as Temporomandibular joint disorder syndrome-TMD, periodontitis) and establish a series of graded diagnostic criteria or rate the reliability of implants based on occlusal analysis. Sorry I don’t know a lot about stomatology.Is there any research on the relationship between occlusal analysis and oral disease diagnosis, rather than just outputting bite force data?Thanks!
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Dear sir, diseases such as Periodontitis, surely have negative effect on the occlusal pressure and will show statistically significant difference in Tek-scan reading in normal patient and patient with periodontitis. However, diagnosing periodontitis only on the basis of occlusal analysis wont be possible. Since, periodontitis is an inflammatory disease and its diagnosis is based on clinical findings such as changes in color, consistency of gingiva, pocket formation and more specifically clinical attachment loss. Depending on the severity of periodontitis, there could be varying degree of underlying bone loss that would affect the tooth's capacity to bear occlusal forces.
Hence, you can compare cases of severe full mouth periodontitis, with bone loss in multipe teeth with those with mild to moderate periodontitis and also keep healthy periodontium as control group to prove that with increasing in severity of periodontitis, the occlusal load bearing capacity is affected.
I hope i was albe to clear you query, let me know if you want to ask something more. We have done a similar study in our department and have published an e-book on the same. Following is the link for same.
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Studying the sensitivity of methods used in HLA typing
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It's very important question
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Cell surface biomarkers are very influential for cell-cell interaction and also for different disease diagnosis
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Have you considered using electronic-nose devices (e-nose instruments) for detecting complex gaseous mixtures of disease (or other surface) biomarker metabolites from headspace volatiles (VOCs) derived from epithelial cells? Headspace volatiles also may be captured into solvents for chemical analyses via GC-MS, NMR and HPLC depending on their molecular weights. I and others have published numerous papers on this approach if you are interested.
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The IoT Enabled ECG Signal Quality Assessment is becoming integral part in the Medical field.
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Check out this article:
Real-Time Signal Quality-Aware ECG Telemetry System for IoT-Based Health Care Monitoring
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Dear Colleagues ..
I'm working on dental diseases diagnosis based Machine learning by using OPG medical images. I need to access real database that includes OPG images. Any Kind replays will be very important to my work and will be very appreciated.
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Hello Sarmad
On this site you can find directly some databases like the ones you want. But, they are very small datasets: http://www.odontosearch.com/datasets.html
I suggest you take a look to the databases that you can find on GitHub. There you will find databases of images related to different diseases. It is possible that you can find the ones you are looking for.
Sincerely
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When I did nested PCR for WSSV diagnosis, i used two tissues gills and pleopod, both are target tissues for virus. I got positive band in the second step only and for pleopod only not for gills. What may be the reason?
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You would need to know whether virus has tissue specific localization as well as whether virus are uniformly distributed among these tissues. If the virus is present in less count in other tissue it may not give you positive amplicons.
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I am working on leaf disease diagnosis using machine learning techniques and looking for hyperspectral of multispectral database of specific crop. Kindly help me and tell the resource from where I can get database which I can use for my research work
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Thank you Sir
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Aspiration pneumonia is a common disease in the world. Clinician may frequently diagnose this disease by clinical symptoms, radiological findings, and lab data. But, I don't know the standard criteria of diagnosing aspiration pneumonia. Does anybody know an international criteria of this common disease ?
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This is a great question. Even pneumonia (PNA) is hard to agree on as a diagnosis now that we can CT the chest so easily and with so little radiation. I think PNA should be looked upon a little like the adage we give our children about words like "stupid" or "ugly". They are each as they do; that is to say "ugly is as ugly does" or "stupid is as stupid does". PNA is as an infection does, not just as an infiltrate exits or as sputum  becomes purulent. The infiltrate may actually be endobronchial secretions on CT and the purulence may be bronchitis rather than PNA. Aspiration PNA is in my practice a pairing of known aspiration tendency and USUALLY a micro bacterial sample that has more than one oral organism at a higher colony count than background. The problem is that chronic aspiration PNA typically starts selecting organisms that are more apt to prevail against others such as staph and particularly MRSA that often is sensitive to bactrim or ciprofloxicin. Also, acute on chronic infections add to the complexity of this care. And lastly, chronic aspiration, if it survived long enough, stops being an infectious process and becomes one that is a source of inanition and cachexia. The host relegates more and more of their nutrition to fighting the chronic airway soilage that they lose reserve and eventually succumb to an infection sometimes years later that results in sepsis and death. This is more common in the post radiation dysfunction of the laryngopharynx rather than in post CVA aspiration, though I have seen this in both. 
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Previously to understand the concept of health disease medical model is applied, where health professional concerned about the disease, diagnosis and drugs, but now this model is replaced by the bio-psycho social model, in which we should include the psychology and social factors along with the biological as it is also the part of the nature.
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Dear Mohan, Nico and Marianne,
Thank you for revisiting these important issues!
I say this because the overlapping fields of psychosomatic medicine, behavioral medicine, health psychology and psychiatric epidemiology have evolved so much since I began my work many years ago. Engel was a pioneer who influenced all of these research areas. Indeed, Marianne's comments are very spot on.
Albee is another pioneer whom you may want to research. I like his work because he originally developed a model that I have found useful in my work on stress in medical students, spouse caregivers of persons with either Alzheimer's disease or schizophrenia, disasters, etc.
Albee formed a simple model that characterizes
Distress as a response to not only stressors, but also a person's unique vulnerabilities and resources.
Distress = (Exposure to Stress + Vulnerability)/Resources
I have found it useful, statistically, to multiply Exposure by Vulnerability because, the product makes it easier to use statistically than the addition.
I am not saying that this is a perfect model, but that it provides a theoretical framework for research in terms of identifying groups at high risk for deleterious responses to varying types of stressors. I do believe, as did Albee, that vulnerabilities are more hard wired (race, gender, developmental illnesses, personality, early trauma) than are resources, which are more flexible (coping, social supports, income).
My experience has been that Health Psychology embraces the other disciplines mentioned above and that it has focused on empirical analyses of theoretical models. Having gone to numerous conferences on psychosom medicine, behavioral medicine, health and physio psychology, I can tell you that it is difficult to talk about health psych in isolation without referencing its cousins. .
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We have diagnosed a girl with McKusick-Kaufman syndrome (MKS) based on clinical presentation with hydrometrocolpos, polydactyly, and congenital heart disease. We want to confirm the diagnosis at the molecular level, but we do not know where to do this.
Has anyone suggestions about laboratories to perform mutational analysis of MKKS gene? 
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Generally, when no need to choose an appropriate model, we can just split data set into only training data and test data. But in medical data mining specifically in prediction, the result is very sensitive to model selection. If the previous works addressed an appropriate model for a specific disease, can we ignore the validation phase and split split data set into only training data and test data?
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It is important to know that data has to be authentic and in a field a like medical science, a slight error may deter you from actual results. It is a better idea to cross-verify the content as a resulting action can do much more!
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The insidious onset of acromegaly causes delay in diagnosis. Given the retrospective nature of the assessment of this delay, all measures are prone to recall bias.
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Sleep apnea and carpal tunnel syndrome are early symptoms of Acromegaly.  The sleep study laboratory screen with IgF 1 levels and has caught occult Acromegaly.  As far as physical findings some individuals just have coarse features, however the one thing I find very helpful is to shake their hand, the dough texture of their hands are unforgettable.
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Need suggestion for a simple clinical study.  eg: malignancy in a patient with pancreatic head mass
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Suggest that you please refer to the following article which may be of some help.
Khanna, AK et al, AgNOR Count and Subjective AgNOR Pattern Assessment (SAPA)
Score in Carcinoma of the Pancreatic Head Including Periampullary Tumors, 
JOP. J Pancreas (Online) 2005; 6(6):575-580.
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can any one clear it?
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ok i can understand your curiosity.
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Hi,
I want to know the relation of Molecular PCR testing and Pathogen Associated Molecular Pattern detection by reproductive immunology for MTB detection in Genital Tuberculosis.
There have been many conflicting view about the test. Recently I have noticed that PCR negative sample often is PAMP positive and clinical there is no MTB symptoms.
Is this largely possible that due to technical constraints a PCR negative sample my come PAMP positive without any clinically relevant symptoms?
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Thanks Werner Solbach. I got the sample where MTB is suspected but other test reports are not avaiable. Realt time PCR came negative but PAMP (test done at other facility) came positive. So i am dillema whether there is some problem with sampling, testing procedure or something else. Is it possible that latent MTB with no active MTB bacteria may give negative PCR but positive PAMP test????
Thank you Ali Abdil for your suggestion and sharing the articles. I read the articles but my answer could not be found from them.
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I want know how many genes for responding cardio vascular diseases, diagnosis and what are the genes expressed  myocardial infarction and coronary artery disease can tell me whom are working these relevant working
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i.e in comparison to DSM-4
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A big change is that there is a depressive disorders chapter. The mood disorders chapter was split with bipolar disorders going to a separate chapter. For MDE itself, the removal of the bereavement exclusion is the biggest change. The actual syndromal definition (5 of 9 A Criteria, at least one of which must be depressed mood or loss of interest) has not changed at all. The bereavement exclusion's removal attracted a lot of discussion, but in my opinion is not a major change in practice or research. Another change is that dysthymic disorder is gone and is now combined with chronic major depression (>2 years) in a new category called Persistent Depressive Disorder.
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I am drawing the Disease-free survival (DFS) and overall survival (OS) curves according to Kaplan–Meier estimates and compared by log-rank tests.
I was using the median as numerical value ( median<High and median>low). But in some paper, they do not use median. I do not have enough knowledge in biostatistics.
Please give me a good advice what is the best way to fix the numerical value to devide High and Low.
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I hope your going to divide disease free survival time then using median is correct
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Dear Colleagues,
I am looking for a person to collaborate with in a research to develop an efficient and a robust automatic method for Malaria diagnosis, which advances the current state-of-the-art technology. The method uses the blood slide images to enumerate the number of infected and healthy RBCs. But, these results should be validated against the traditional approach to diagnose Malaria.
Currently, the model has been theorized and what is missing is to have as many experiments as possible from real lab data. In the future, the research may proceed to incorporate the proposed model into some portable devices, like mobile phones, such that individuals may be able to self-determine their parasitemia levels without visiting hospitals (in situations where patients do not have access to hospitals).
Just as a disclaimer, the research is not funded by any Organization or Company, and is personal for now. The only thing I can guarantee at the moment is co-authorship (if an individual would like to be included in the authorship page). We are about four engineers, and more details of the method may be revealed when we set some terms with a volunteer. Below are the things we specifically require in the research:
  • A Person who will have access to about 200 blood-smear images from suspected patients of Malaria.
  • A person who can perform a manual count of the infected RBCs, and total RBCs, per every sample image
  • A Person who may provide his time to review the final paper, particularly in a section with medical information, to ensure that it seats well before submission.
Just for emphasis, now the research is in the simulation stage; the later stage of implementing the algorithm in the real hardware will be considered the next phase of the research.
Anyone with interest, you are kindly welcome. You may either use this forum for communication, and all comments will be noted. Alternatively, you may use private message me
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Dear Baraka Maiseli,
Your proposal it is very interesting, I have a team (3 researchers) with strong experience in microscopic diagnosis of malaria and count of infected RBCs (principally malaria falciparum and vivax). Thus, we were the authors of the Manual of Malaria Diagnosis and Treatment in Bolivia, that include modifications in the antimalarial drugs policies and normalization of malaria diagnosis procedures as (past) head of the National Reference Laboratory in Malaria. We have experience in “in vivo” surveillance of the antimalarial-resistance, malaria surveillance and other similar topics including molecular markers related to antimalarial-resistance. In this way, probably we have the characteristics to satisfy your specific requirements.
We need more information related possibilities to work in collaboration with you and other researchers. Preliminary, I confirm that we are very interested in your innovative proposal.
Sincerely,
Eddy Martinez
DIRECTOR
INSTITUTO DE INVESTIGACION EN SALUD Y DESARROLLO
FACULTAD DE MEDICINA
UNIVERSIDAD MAYOR DE SAN ANDRES
LA PAZ, BOLIVIA
PAST-COORDINATOR OF AMAZON MALARIA INITIATIVE /AMAZON NETWORK FOR SURVEILLANCE OF ANTIMALARIAL-DRUGS RESISTANCE (AMI/RAVREDA)
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I'm facing two different questions.
(1) Are plants produced by conventional breeders' breeding processes (i.e. cross, select, etc), patentable? This is now before the EBA at the European Patent Office (I believe G-1/98 is wrong), and 
(2) Does a method of diagnosis in medicine have to be of a disease condition, and does there have to be a cure available if suffering from the condition is diagnosed? (I believe G-1/04 is wrong). This is now the subject of an opposition.
On both, I have to put my case together in the next week or so. In neither case do I have a financial interest - I just want the law to get back on track.
Can anyone help? Is anyone willing to help? More particularly, are there any non-OB/GYN medics out there who would be willing to talk?
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The current legal status of these issues at the European Patent Office is as follows.
(1) Are plants produced by conventional breeders' breeding processes (i.e. cross, select, etc), patentable?
According to Art. 53(b) of the European Patent Convention, plant or animal varieties or essentially biological processes for the production of plants or animals are excluded from patentability. In the past, there has been questions on what exactly is means by "essentially biological processes". This resulted in two referrals to the Enlarged Board of Appeal of the European Patent Office (G 2/07 and G 1/08) which then made the following statements.
1. A non-microbiological process for the production of plants which contains or consists of the steps of sexually crossing the whole genomes of plants and of subsequently selecting plants is in principle excluded from patentability as being "essentially biological" within the meaning of Art. 53(b) EPC.
2. Such a process does not escape the exclusion of Art. 53(b) EPC merely because it contains, as a further step or as part of any of the steps of crossing and selection, a step of a technical nature which serves to enable or assist the performance of the steps of sexually crossing the whole genomes of plants or of subsequently selecting plants.
3. If, however, such a process contains within the steps of sexually crossing and selecting an additional step of a technical nature, which step by itself introduces a trait into the genome or modifies a trait in the genome of the plant produced, so that the introduction or modification of that trait is not the result of the mixing of the genes of the plants chosen for sexual crossing, then the process is not excluded from patentability under Art. 53(b) EPC.
4. In the context of examining whether such a process is excluded from patentability as being "essentially biological" within the meaning of Art. 53(b) EPC, it is not relevant whether a step of a technical nature is a new or known measure, whether it is trivial or a fundamental alteration of a known process, whether it does or could occur in nature or whether the essence of the invention lies in it.
Following this case law, one cannot patent plants that are produced by conventoinal breeders' breeding processes. However, one can protect such plants using the breeder's right.
Unfortunately, this is not the end of my answer, since meanwhile new questions have been referred to the Enlarged Board of Appeal in G 2/12 and G 2/13. These questions are the following.
G 2/12:
1. Can the exclusion of essentially biological processes for the production of plants in Art. 53(b) EPC have a negative effect on the allowability of a product claim directed to plants or plant material such as a fruit?
2. In particular, is a claim directed to plants or plant material other than a plant variety allowable even if the only method available at the filing date for generating the claimed subject-matter is an essentially biological process for the production of plants disclosed in the patent application?
3. Is it of relevance in the context of questions 1 and 2 that the protection conferred by the product claim encompasses the generation of the claimed product by means of an essentially biological process for the production of plants excluded as such under Art. 53(b) EPC?
G 2/13:
1. Can the exclusion of essentially biological processes for the production of plants in Art. 53(b) EPC have a negative effect on the allowability of a product claim directed to plants or plant material such as plant parts?
2. In particular:
(a) Is a product-by-process claim directed to plants or plant material other than a plant variety allowable if its process features define an essentially biological process for the production of plants?
(b) Is a claim directed to plants or plant material other than a plant variety allowable even if the only method available at the filing date for generating the claimed subject-matter is an essentially biological process for the production of plants disclosed in the patent application?
3. Is it of relevance in the context of questions 1 and 2 that the protection conferred by the product claim encompasses the generation of the claimed product by means of an essentially biological process for the production of plants excluded as such under Art. 53(b) EPC?
Hence, your question can currently not be answered completely. We will have to wait and see as to what the Enlarged Board of Appeal will decide in these matters.
(2) Does a method of diagnosis in medicine have to be of a disease condition, and does there have to be a cure available if suffering from the condition is diagnosed?
The answer to this question is simpler. For patentability, methods of diagnosis are similar regardless the technical field. Hence, it does not matter whether the method of diagnosis is in the medical field or not. Even if it is in the medical field, the diagnosis does not necessarily have to deal with a disease condition, as long as something is diagnosed. Also, a cure does not have to be available. For example, a method for diagnosing whether a certain individual suffers from ebola may be patentable, even though currently no cure exists.
There is case law on methods of diagnosis however, since Art. 53(c) EPC states that methods for treatment of the human body by surgery or therapy and diagnostic methods practised on the human body or animal body are excluded from patentability. With respect to diagnostic methods under Art. 53(c) EPC the Enlarged Board of Appeal listed the following requirements in G 1/04.
1. In order that the subject-matter of a claim relating to a diagnostic method practised on the human or animal body falls under the prohibition of Art. 52(4) EPC [now Art. 53(c)], the claim is to include the features relating to:
(i) the diagnosis for curative purposes stricto sensu representing the deductive medical or veterinary decision phase as a purely intellectual exercise,
(ii) the preceding steps which are constitutive for making that diagnosis, and
(iii) the specific interactions with the human or animal body which occur when carrying those out among these preceding steps which are of a technical nature.
2. Whether or not a method is a diagnostic method within the meaning of Art. 52(4) EPC may neither depend on the participation of a medical or veterinary practitioner, by being present or by bearing the responsibility, nor on the fact that all method steps can also, or only, be practised by medical or technical support staff, the patient himself or herself or an automated system. Moreover, no distinction is to be made in this context between essential method steps having diagnostic character and non-essential method steps lacking it.
3. In a diagnostic method under Art. 52(4) EPC, the method steps of a technical nature belonging to the preceding steps which are constitutive for making the diagnosis for curative purposes stricto sensu must satisfy the criterion "practised on the human or animal body".
4. Art. 52(4) EPC does not require a specific type and intensity of interaction with the human or animal body; a preceding step of a technical nature thus satisfies the criterion "practised on the human or animal body" if its performance implies any interaction with the human or animal body, necessitating the presence of the latter.
I hope this information is helpful to you.
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Fuzzy logic expert system for diagnosing a disease.
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Hi
I think Matlab, too
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Is a measure of zero LDL good for you?
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In addition to the above answer of lab artifact or error, a sudden decrease may be due to a reaction in the blood which precipitates the LDLc with WBC’s and therefore, it would still be artificially low due to bound LDLc in vivo.  
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Looking for clinical, macroscopic and microscopic signs.
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hi Ferdinand
thank u for suggest !
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Diagnosis of infectious diseases
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If you're using a test that has a sensitivity of for instance 90% and specificity of 80% to test for the presence of disease X in a population of 1000 individuals. Let's say 240 individuals have a positive test and 760 individuals have a negative test result, the true prevalence of disease X in this population is given by: (apparent prevalence plus the test specificity minus 1) divided by (the test sensitivity plus test specificity minus 1). In the example above, this works out to be (0.24 + 0.8 - 1) / (0.9 +0.8 -1) = 0.04 / 0.7 = 0.057. Therefore, given a test sensitivity of 90% and a test specificity of 80%, the true prevalence of disease X in this population is 0.057 (5.7%) i.e. 57 individuals are truly diseased but since our test only has a sensitivity of 90% this means that only 90% of the 57 will be correctly diagnosed as being positive (51) while 188 will be false positives. Similarly, 80% of the disease negatives will test negative (754) while 6 individuals are false negatives.
Hope this is helpful
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I want to know its limitations, as argument for developing improved method.
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Hi there
In fact, there is no state-of-art diagnostic method available for diagnosis of fasciolosis. Serology help to some extent but its main limitation is that antibody will stay in the serum for rather long time after recovery. Microscopy is good but when the worm is too young or too old, egg cannot be seen in the stool. Furthermore, the eggs in adult worm are passed intermittently and may not be diagnosed on routine fecal examination. There are also coproantigen detection assay and also PCR. Both are relatively reliable approaches for diagnosis of fasciolosis.