Questions related to Diabetes Drug Development
Diet is known to alter the gut microbiota. Is it likely that processed foods and high glycemic index foods may bring about such a change which in turn may lead to inflammatory and other changes progressing to diabetes?
I am looking for papers or evidences for loss of insulin receptor in case of diabetes. I am interetsed to know more about insulin resistance development based on insulin receptor loss. Please give your comments on the same.
Thanks and reagrds,
Is there any literature available showing GLP-1 concentrations in humans with Metabolic Syndrome?
Equally is there any data in human showing DPP-4 concentrations in humans with Metabolic Syndrome?
Hi Everyone I am looking for antibodies for ER pathways that works in INS-1 cells or any other cells. There are lots of paper out there but I want to know if anybody have tried these antibodies in INS-1 cells and that has worked perfectly. The antibodies I am interested are as follows:
spliced-XBP1. p-PERK, PERK, p-IRE1 alpha, Total-IRE1 alpha, ATF4, ATF6 active.
Any information will be highly appreciated. Thanks in advance.
Hi! I am looking for diabetes patients' data set right from symptoms, physical examination, lab investigations,family history and risk factors. Can anybody help me ? We are building the physician recommendation system and currently we are focusing on diabetes for the pilot application. We can't proceed further due to lack of data. I would really appreciate if you can share or help me directing where can I find the data that I need in order to proceed further. Please help.
please, can somebody explain how to carry out molecular docking studies on bioactive compounds against diabetes and cancer?
I am doing antidiabetic activity of plant derived compound in Sprague Dawley rats. After the alloxan treatment, I will administer my drug in particular concentration. After certain period of time, I will check the blood glucose in tail vein blood using glucometer. And also, after sacrificing all animals, I will have to estimate serum glucose concentration by the glucose oxidase method. What might be the difference between the estimation of glucose in whole blood using glucometer and estimation of glucose in isolated serum or plasma by using glucose oxidase methods? What is the normal range of both in rat samples? Will you please help me to clarify this my basic doubt?
what is the best biological procedure proving the action mechanism of a drug on PPARgamma and sulfonylurea receptor as antidiabetic agents?
I need papers about the effect of the acute effect of metformin in rodents as well as the chronic effect. I need to know which doses they use and which rout.
I am attaching here with the details related to Indo- European Call on Health-“Diagnostics and interventions in chronic non-communicable diseases”
Please go through it. If anyone / professor / researcher, interested, can make collaborative proposals.
Semi Conductors Nano structures
Hybrid magnetic-semiconductor structures & Bio-nanomaterials
I have some experience / research interests in above fields. semi-conductor / magnetic / super-paramagnetic materials are useful in making bio-sensors / diagnostics. .
Also, I am attaching here with my recent achievements in nanotech field for references. http://goo.gl/jAFeCV
I have a very controversial question. Is it possible that a compound increases chop in a dose dependent manner in parallel decreases all other markers for apoptosis such as Cleaved caspase-3, Parp and TXNIP. I have read couple of post indicating Chop not being the best marker for apoptosis but can chop be cytoprotective? Comments will be highly appreciated. Thanks
I have read a number of previous original research (including some reviews) that analysed the association between metformin use and risk of lactic acidosis.
In almost all studies, the outcome has been zero. However, there are some few case-control studies (and case series) that showed the likely association.
Is there anyone aware of conference abstracts/unpublished data regarding this aspect? Kindly share.
Elderly patients may have reduced renal function induced by age associated changes ( measurable ) and possible other changes. All these, before comorbid conditions and polypharmacy. Please share your experience and opinion regarding the use of Metphormin as treatment of Diabetes mellitus in old age ?
I would like to know if anybody has any experiences with diabetes prevention programs in Community Pharmacies, something like Findrisc or similar. I would be most grateful for any information you have.
What are the solvents used to dissolve the standard (Diazoxide, Glibenclamide, Sulphanylurea) drugs for insulin assay of Pancreatic beta cells of rat growing in RPMI media?
I am performing a standard insulin assay using the Mercodia insulin kit. The cells are being grown in 96 well plates at 50k cells per well.
Please also let me know the concentration of these drugs in the standard assay and cell number used per plate.
If possible, please also send me a few good but simplified papers on insulin assays.
I tried to induce diabetes in hamsters with high cholesterol diet by STZ injection. I used a dose of 50mg/kg BW for 3 consecutive days at non-fasting status. My hamsters died in the following 2 weeks. The remaining were high in plasma lipids and glucose with very bad health condition. How can I improve my protocol and decrease the mortality? Thank you
The compounds are synthesized by interaction of 2 materials together. The resultant legends molecular weights were calculated from its structure. I need to examine whether it will act as anti-diabetic or not?
All I have is the molecular weight
Protocol for water deprivation test in patients with Diabetes Insipidus
Different books have different protocols
Dose of Vasopressin varies from 5U to 1U/m2
I am planning work aimed at evaluating hypoglycemic effects of camel milk. Main aim is differentiating effect due to high insulin content (DM Type I) or other hypoglycemic effect (DM - Type II). The plan is to induce DM pharmaceutically and assess potential effects of camel milk consumption on plasma glucose levels.
I want to study mode of action of a medicine used in traditional system of healing and want to check its mode of action. I will be doing my studies on mice models but do not wish to study mode of action in vivo. can HeLa cells be used?
hello, I recently was trying to make type II diabetic rats model by i.p injection STZ (65mg/kg). But the rats was dying in 5 days even I tried to give little insulin(4 IU) everyday since day 3 to control the deteriorated rats (blood glucose > 25) . Am I missing some important issue during performing animal model and how to increase the survival rate? Could anybody give some advice on this?
I am working on a molecule that stimulates insulin secretion but does not increase calcium influx in cultured pancreatic beta cell (MIN6 cell).
I wanted to know which is the standard protocol that is followed. Hence, I wanted to know which is the standard protocol that is followed.
I want to do some type 2 diabetes studies in vitro for some homeopathic product to know the potency of the drug.
Streptozotocin destroys completely the beta cells in islets of langerhans (Mitochondrial DNA damage) and my doubt is whether the drugs/herbs can rejuvenate the dead cells?
From DPP study we know that metformin is effective for prevention of diabetes. Major recommendations like ADA standard of care 2013 advise using metformin for high risk patients. Beside biguanides there are evidence that other classes of anti-diabetic medicine (Alpha-glucosidase inhibitors, and thiaolidinedionses) that are also effective for prevention of diabetes. Is there any recommendation for usage of these drugs for preventive measures?
Kindly suggest the moderate dose of STZ to induce diabetes in Rattus norvegicus for evaluating antidiabetic activity of herbal plants. We administered STZ with sodium citrate buffer (4.5 pH) with a dosage of 35 mg/kg body weight of rat through i.p at single dose. But the fasting blood glucose level was increased >500 mg/dl on the 3rd day some rats (mostly male) were dead 5 days after i.p injection of STZ.
This question is just out of curiosity and should not be perceived as mindless promotion of strange ideas for diabetes therapy.
Can TRIS, a common base that we all use for preparing buffers be used for prevention of postprandial hyperglycemia or diabetic phenotype contributed by sugar from digestion of disaccharides?
Usually we consider the chemically induced diabetes for experimental purpose to evaluate the antidiabetic activity of herbal drugs and STZ is preferred, but the mechanism of type of diabetes is not clear to me, any suggestions are appreciated.