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hi
can anyone introduce a new technology or an article about ruminant feed, please?
thank you.
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@Juan Carlos Blandon
You're right.
Thank you.
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How this system works, how to apply, what kind of terms should be provide to suit the system? Thank you
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This number is suitable for cattle comfortable temperature (generally 15 to 18℃), house feeding, light activity, no stress environment. When the temperature is below 12℃, the maintenance energy needs to increase by 1% for every 1℃ decrease. Net energy for weight gain (kj) of growing-finishing cattle = (2090+25.1W)X daily gain /1- (0.3x daily gain) [where W is body weight (kg)
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I am working on coating nitrate to make it slow-release. I have made a primary product. First, XRD and IR tests were conducted to assess that. Then, I performed a pre-test to investigate its release in the rumen fluid using a "Nitrate/Nitrite Colorimetric Assay kit. However, the kits available in Iran are made for measuring nitrate and nitrite in water.
So, I would like to know your opinion and suggestion about how to measure nitrate and nitrite.
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Dear colleague
please check:
Regards,
Redimio
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My advisor sent me the following code and told me to rewrite it in R.
proc nlin;
parms a=19 c=9 k=.08 lag=2;
*a=soluble, c=undegradable, k=rate/h, lag=lag time;
time=time-lag;
if time<0 then time=0;
b=100-a-c;
model DM=b*exp(-k*time)+c;
output out=temp p=Predicted r=Residual;
The non linear model was easy enough but I was having issues with fitting lag time. However I have written a function that works great, and I have posted it here so that it will hopefully help someone else in the future.
output1<-NULL
finaloutput<-NULL
degradfun<-function(x){
data1<-subset(DegradADJ, Subset_Term==x)
parms=list(b=100,k=0.04,c=0, lag=15)
#m<-nls(N_Disapp~b*exp(-k*(Hour-lag))+c,data=data1,start=parms)
m <- nls(formula = N_Disapp ~ ifelse(test = lag >= Hour, yes = b*exp(-k*(0))+c,
no = b*exp(-k*(Hour-lag))+c),
data = data1, start = parms)
out<-summary(m)
print(summary(m))
data1$predicted<-predict(m)
plot(data1$Hour, data1$N_Disapp, main=x)
print(lines(data1$Hour, data1$predicted, col="blue"))
output1<-data.frame(b=out$parameters[1,1], k=out$parameters[2,1], c=out$parameters[3,1], name=x)
finaloutput<<-rbind(output1, finaloutput)
}
To run a loop for all the products:
AllIDs<-unique(DegradADJ$Subset_Term)
lapply(AllIDs,degradfun)
Note: finaloutput will contain a table with all the results
To just run one:
degradfun("Product1")
If this helps you I just ask that you "recommend" this post. Thank you.
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I think, parms a=19 c=9 k=.08 lag=2;
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Hi everyone,
I am doing an allantoin assay in urine and all the protocols seem pretty vague. I am currently using this website which seems to be the most helpful:
It mentions that I use 15 mL tubes, but since I am boiling the samples should I use glass tubes with a cap to prevent escape? Or can I just use plastic tubes like the ones here:
Thank you!
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Nice question
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In ruminants, nutrient inputs are first to fermentative digestion by ruminal microorganisms. The microbial fermentation products eventually become available as energy (volatile fatty acids) and protein (microbial cells) for animal tissue metabolism. However, ruminal fermentation also produces methane (CH4), carbon dioxide (CO2) and ammonia (NH3). Methane production in the rumen is an energetically wasteful process, since the portion of the animal’s feed, which is converted to CH4, is eructated as gas.
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There are many problems in the ruminants, from birth to rumen development. Ruminants have a groove called esophageal, which is a stretch of the esophagus or a tube that bypasses the retina and the groin and enters directly into the paper and primary function of the groove in small ruminants to allow the milk to museum directly from the esophagus To the paper without passing through the rumen and the curriculum
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To determine the costs of feed in israeli milk production I am interested of the amount of roughage, TMR and byproducts that an average dairy cow consumes in Israel.
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Please have a look at the following link and PDF attachments.
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I am wondering if someone can advise on the best feeding regime for Red Mbororo cattle in the tropics.
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The question has two parts Ancient knowledge regarding :First: 1- The precursor of milk is digested food or excretions in the stomach. Second: The role of blood in circulating the components required for milk formation. 
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The association of blood and milk considered here in the Qur'an and Talmud was no more than a fortunate guess based on the fact that both blood and milk appear to be body excretions-it is not evidence of biological knowledge.
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I am asking for any help regarding the feasibility of on-site classification of antral follicle count in cows and buffaloes?
Can we perform it at any time during the estrous cycle? if so, which is the most appropriate period during the estrous cycle to perform US examination (PRO-estrus-or diestrus).
I red this updated abstract but unfortunately, the full-text was not available.
Association between antral follicle count and reproductive measures in New Zealand lactating dairy cows maintained in a pasture-based production system.
Martiniz MF, Sanderson M, Quirke LD, Lawrence SB, Juengel JL
Theriogenology. 2016 83 (3): 466-75.
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Ok So you need to use either a 5MHz or 7.5MHz linear rectal probe - any suitable scanner machine should work. We use an Aloka 500, but there are lots of options! Depending on your scanner type you may be able to capture digital images, i.e., record as you scan; or you may need to link up to a recording device. For cows if you can get all animals to day 1.5 or 2 post oestrus and then count all follicles greater than or equal to 5 mm. I would set a marker at 5mm on the scanner and then count those greater than or equal to that size as I rotate the ovary under the probe. It is best to do your counts live as you scan and then use your video records as a back up or to check if there is something you are unsure of afterwards. By counting live you will follow all ovaries to find all the structures on the ovaries - not really possible to do afterwards with a recording!
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Tell me your views in this sense for both dairy and beef cattle.
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Yes .we fed diets that is concentrated.
Regards
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Anyone could provide data regarding the absorption/metabolisation of propylene glycol in powder against liquid form?
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Food and protozoal N and residual N are normally calculated while knowing the effect of different feeds on rumen fermentation patterns
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Dear Ravi
Please go through the reference cited by Zahira. It is very helpful. I have read it before and found it to guide me very precisely.
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I want to know the best age of insemination of heifer buffaloes?
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Hi
In my opinion, reaching optimal physical condition is more important than age. I agree with Mr. Munir. You should consider all items
Good luck
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I've been reading a few things about adding salt to the grass silage, however, I was unsure if this addition will increase the cow's propensity for milk fever. I ask this because by adding Na (which is a cation) we are increasing the DCAB and elevated DCAB is supposedly related to the occurrence of milk fever.
I found that the grass silage has around 0.29% Na in DM and that in order to have a low risk of milk fever the Na content should be 17% in DM.
What can you say about this?
Thank you
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But adding salt to silage will surely affect the quality of silage as well. As you know that salt is also have moisture absorption capacity .By adding salt will reduce the moisture level of grass then the recommended level that can affect silage fermentation process/quality .Think for it as well am i right.
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Does it make sense taking into account that humans need DHA and EPA and cows scarcely convert C18:3n3 into them?
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You may want to check Valorex, French company using extruded linseed
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It would be useful to receive information on the dosage of specific compounds (C18:3n3, DHA, EPA, CLA, etc.) as well of their source (lineseed and others)
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I think that you can find your response in attached paper. It should be noticed there is no information about daily requirement of essential fatty acids (18:2+18:3 and Omega-3) in Ruminants. For example, recommendation for linseed inclusion to diet (for improving reproduction performance or modifying milk FA) is up to 10% of DM (you can refer to Petit et al., 2010- Canadian Journal of Animal Science). of course there are a lot of papers and experiments in the case of unsaturated fat supplements and milk fatty acid profile, however, we should know the production of a high quality milk (rich in special FA) is not similar to essential FA supply and their metabolism in body.
In the case of interactions between CLA supplementation and reproduction in dairy cows, the results are inconsistent. and you can not find a common recommendation in order to promote reproduction or BW changes and etc when cows fed a CLA supplementation.
In fact effective dosage for omega-3, omega-6 and CLA is related to lactation stage and ration components (forage:concentrate, starch level, particle size and peNDF , etc), although physiological stage is determining addition of omega-3 or omega-6 to dairy cow diet (for instance, omega-6 sources from parturition until 60 DIM and Omega-3 from first service because of improve pregnancy outcomes at first service).
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Hey to all!
Hope this message finds you doing well. I'm writing to ask you about the correct time of blood sampling to recognize how DCAD diets work? As it is believed "nearly all cows will experience some degree of hypocalcemia at the onset of lactation (Goff, 2014)". So, when should we draw blood to assay this problem? How many percent of cows should have normal Ca concentration (> 8.5 mg/dl)? New data from Cornell University also show that more than 55% of cows are hypocalcemic on d 1 after calving even on DCAD diets. I could not find any answer to this question. Could you please advice me on this issue?
Regards,
Ehsan
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Ebrahim,
Thanks a lot for your adding up. I just found an alarm level of 30% recommended by Dr. Oetzel. But, it is for the blood samples drawn on the calving day. Considering what you said and other papers, I think 48-72 h is great to take blood samples and if more than 30% of cows are hypocalcemic, we should re-look at our DCAD program. Let me know what you think.
Cheers,
Ehsan
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i wish to know how cassava foliage  can improve milk yield in dairy cattles
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Hi Okunlola,
I do not know if cassava chip would be interesting for you. Check papers on cassava chip effects on milk production due to its content in malate (papers published in 2006and 2007 by Wanapat if I am not wrong).
In this sense, also the attached article could help you.
Regards.
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I'm looking for references about milk production response to protein supplementation (preferably rapeseed/canola meal but other protein feeds do as well) at the end of lactation (around 200 days in milk, or so). Most of the studies are conducted with dairy cows at early to mid-lactation, so end-lactation studies are very difficult to find. Could anybody help me, please?
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MTP:n julkaisu minulla jo on, mutta en tiennyt että siitä on eng.kielinen konferenssijulkaisukin. Se kelvanneekin lähteeksi artikkeliin, jota parhaillaan kirjoitan. Kiitos :)
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Dear peers,
  I need to understand whether uniform pellet length vs. uneven length with similar ingredient composition has any impact on dairy cattle/buffalo performance?
  If so, please provide its scientific basis with relevant literature link.
Thanks!
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The length and diameter of the pellet will affect the palatability  and nutritive value of the pellet depending upon the animal species you are targeting for feeding. In smaller animals, the pellets of lower dia and length will be efficiently utilized whereas the pellets of higher length and diameter will be difficult to swallow and chew and hence the utilization will be poor.
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I want to supplement dairy cows' diet with rumen protected Met to enhance milk protein synthesis. I seek an efficient feed additive with sufficient protection in the rumen to use as a co-supplement with RP-Met (in synergism with together). a significant increase in milk protein (protein fractions) is expected as research target. 
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Have you looked at essential oils? I know that CRINA products from DSM increases protein bypass (used to work for them)
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Most choline supplementation to ruminants is made throug 'rumen-protected choline' (RPC). There are various forms of RPC in the market, with different concentrations of choline chloride, different rumen by-pass performances, and also different release levels during post-rumen digestive tract.
In cases where rumen-protection is innefective, the majority of the choline chloride fed to animals will be degraded by rumen microbiota into TMA, and at further stage into methane. But before degradation into methane, there may be a negative impact of increased TMA concentrations in the rumen.
At post-rumen digestive tract an excess of choline cholride may have a similar fate. While ruminants will benefit from choline supplementation, if the amount made available at the gut is much larger than absorbed (required) levels, the excess tends to be converted into TMA by anaerobic microbiota.
I am looking for references on choline degradation into TMA in ruminant animals, and to which extent the amount of TMA formed may become a negative factor instead of the intended nutritional benefits of choline supplementation.
Thanks and regards to all,
Jorge
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Thanks for the references Stalin, and yes, they did help.
Considering that rumen-protected choline (RPC) chloride is nowadays standard in ruminant nutrition, to avoid ruminal degradation as reported on the provided references, I'm still interested to learn about excessive supplementation at intestinal level.
Daily natural choline intake vary widely depending on fodder composition, and technical documentation from various RPC providers also indicate a large range of suggested daily supplementation, so the possibility of an excessive dosage is not to ignore on the high-production dairy cows.
What is the fate of choline chloride that escapes ruminal degradation but, being fed in excessive amount, won't be incorporated into expected metabolic pathways?
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In the feed ration the percentage of dry substance is 50.42%. I need it in order to calculate the emission of Nitrates, NH3 and Phosphates in the air/soil and groundwater by the application of manure as fertilizers. 
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Hi Lapo,
Here attached, a simple spreadsheet with calculations that you can follow easily.  There are two references, based on which the estimations were done.  They are listed in the spreadsheet.  If you cannot access these references, I can send.  These references have few additional equations that can be used to predict the values you are interested in, according the type of data available with you.
One important thing to remember is: any prediction is associated with certain amount of error.  Prediction errors for these eqs are about 10-20% of the predicted mean.  You can read further from the references listed.  Hope this is useful.
-SJ
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How to explain that ,NDF/ADF digestibility in dairy cattle was improved while cellulolytic bacteria numbers was decreased? Anyone can explain or have some suggestion/ reference?
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As an engulfing manner of protozoa against bacteria in addition of bacteria counting you must pay attention to protozoa. although protozoa have lower number compared to bacteria, as weight they are mostly same as bacteria.
So it's possible observed decrease in cellulolytic bacteria population was due to increase in size and number of protozoa.
also check microbial protein  markers. please find and review Dr. Dehority's book on rumen microbiology. it would be very useful reference.
however as others said above don't forget ruminal pH functions. ch13 of attached book may be useful.
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i need other than DCAB balance And to be  kept on a low calcium diet before parturition 
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Hello Ahmed M. Abd EL-Hafiz,
I actually Understand as you know more about milk fever, But for more clarity, let me stand from what the disease Milk fever mean!
Milk fever  is a condition of older, third to sixth lactation, high-producing dairy cows associated with Loss of blood calcium through milk within the first 2-3 days of lactation. This Metabolic disease has three progressive stages
Stage one
-  Cows are able to stand but show signs of hypersensitivity and excitability.
- Cows may appear restless and bellowing.
- If calcium therapy is not instituted, cows will progress to stage two.
 Stage two
- Cows are unable to stand but can maintain sternal recumbency.
- Depression, anorexia, dry muzzle, abnormal body temperature, and cold extremities are seen.
- Cows often tuck their heads into their flanks or, if the head is extended, an S-shaped curve to the neck may be noted.
 Stage three
- Cows lose consciousness progressively to the point of coma.
The Prevention of this metabolic disease lays on Three things:-
1.       Managing cows at dry period by keeping them with low level of calcium so as to stimulate the calcium regulatory system from their bones. If we do this, they can mobilize their body calcium during calving when there is shortage.
2.      Since Milk fever is the result of low blood calcium level in the body during calving, providing high calcium level forages like Lucerne within the first weeks of lactation can be preventive mechanism.
3.      Lastly, Cows with high risk can be injected with vitamin D3 within 2-8 days prior to calving.
For more information, you can see the following links:-
Actually there are alot of articles regardng Milk fever
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I am doing a trial on the effect of phase feeding on the reproductive performance of the Fiji sheep ewes under grazing condition. I have already flushed the ewes with brewers grain (dried) for a week, mated them from late November to December, 2015. Currently awaits for their 70 day after mated for feeding.
From 0 - 70 day mated, no feeding, at 70 day feeding starts (only brewers grain), at 100 day feeding (brewers grain and copra meal), at 120 day feeding (only copra meal with additional calcium) till lambing.  
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You can achieve a good Ca:P ratio by adding 7,5 units of ground limestone with every 100 units of Brewers grain.  And when feeding 100 units Brewers grai plus 120 units of Copra meal, add 10 units of limestone. This shold give a Ca: P ratio in the vicinity of 2,28:1
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.
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You mean that if standard dose rate is 100 gm , one should feed 125 gm?
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Teat ends look great. Any other reason?
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1. Teat sphincter always take sometime to constrict the teat after milking and moreover  100% evacuation of milk is not done during milking. So in these conditions the remnant milk will be effluxed out during resting.
2. When the udder is filled with milk, the intramammary pressure will be at peak. During these times, if animals rests, then the pressure will be increased further and force the sphincter to be relaxed. 
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Actually, monensin would decrease ammonia-N concentration in the rumen, however, my research found that ammonia-N concentration was increased when supplement monensin in diet at 33mg/kg diet in cattle. How to explain?
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the pure cultures of rumen bacteria with the highest ammonia production from protein were gram negative and produced only 75-80% of the ammonia produced by mixed rumen bacteria. Russell’s group in the 1980s hypothesized that within the rumen unknown strains of bacteria that have high specific rates of ammonia production must exist.
 Yang and Russell (1993) demonstrated that the decrease in rumen ammonia
caused by monensin was associated with a 10-fold decrease in ruminal bacteria that use amino acids and peptides as an energy source for growth. See attached paper.
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Dry or powder Propylene glycol vs its liquid form. Is there is any difference regarding ABSORPTION/METABOLISM?
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Thank you very much dear Ebrahim. However, as you can see in my question, I was asking not about management but about  absorption/metabolism. Could you provide me with any data in this regard?
Thank you very much.
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Has anyone ever used the ELISA technique for the detection of pathogens in milk of cows with mastitis? There is too little bibliography.
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Hola Luis! Gracias por darme una mano! Mi mail es ncaggiano@fvet.uba.ar, espero tu información! Nuevamente gracias! Saludos.
Nicolás
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i need measure total-tract apparent digestibility in dairy cow with iNDF?
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Dear Luo,
Attached file may be useful for your question.
Best wishes,
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We use selenium to improve semen quality, and now we want to use nano-selenium
In your opinion, what percentage  concentration of organic selenium must be use now
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as such there is nio recommendations for any nano minerals in livestock and poultry feeding. we have to see differented works and reports and like wise we have to frame our research and levels of inclusion.
regarding nano Se
here is one article for your reference
Mohapatra, P. and Swain, R. K. and Mishra, S. K. Effects of Dietary Nano-Selenium on Tissue Selenium Deposition, Antioxidant Status and Immune Functions in Layer Chicks. Iternational Journal of Pharmacology, 10 (3). pp. 160-167.
Full text not available from this repository.Abstract
Comparative study on the effect of nano selenium (nano-Se) and sodium selenite on the growth, bioavailability, antioxidative activities, hematological and biochemical parameters, cellular and humoral immunity was done in layer chicks upto 8th week post feeding. The results showed significant differences (p<0.05) in relative weight gain and final body weight of the nano-Se treated groups upto a dose of 0.3 mg kg(-1) of diet as compared to sodium selenite and control groups. However, further increase in dietary nano-Se content in feed had negative effect on weight and Relative Gain Rate (RGR). Survival rate and Feed Conversion Ratio (FCR) were not affected by dietary treatments. Chicks fed with both nano-Se and sodium selenite showed higher (p<0.05) Se content in different tissues (breast muscle, liver, kidney, pancreas, serum and feathers). However, highest value (p<0.05) of Se content in breast muscle and liver was observed in nano-Se treated groups. Selenium concentrations in serum, liver and breast muscle increased linearly and quadratically (p<0.05) as dietary Se level increased for all Se sources but its magnitude was substantially greater (p<0.05) when nano-Se was fed. Glutathione peroxidase (GSH-Px), erythrocyte catalase (CAT) and superoxide dismutase (SOD) activities were significantly different (p<0.05) in all treated groups than control. Dietary nano-Se also increased several serum biochemical and haematological parameters. In addition, it significantly increased both cellular and humoral immunity in layer chicks after 8th weeks of post feeding. In conclusion, dietary administration of nano-Se was found superior than that of inorganic sodium selenite in various aspects in layer chicks. Further extensive study for exploring absorption mechanisms, metabolic pathways, ideal dose/form of nano-Se is suggested for optimum utilization of nano-material based application of Se feeding in poultry.
Item Type:
Article
Subjects:
Molecular Biology
Depositing User:
Unnamed user with email alok@urdip.res.in
Date Deposited:
27 Mar 2015 06:30
Last Modified:
27 Mar 2015 06:30
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hello every body, i want to know anything about the application of nano technology in the dairy animal feeding
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It may be used in protected mobilization molecules lipo-soluble vitamins, chelated selenium and hormones
See: Nanotechnology in agri-food production: an overview, Nanotechnol Sci Appl. 2014; 7: 31–53
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High water table in places where the population of cattle is more?
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There are more interactions between groundwater table, grass and cattle. In the temperate zone (or equivalent elevation belt in the Himalayas) a high water table in winter slows the grass growth in spring. A permanent, rain-dependent high groundwater table creates peat conditions avoided by cattle. Further, interaction of fire, grass and bush encroachment is common grazed floodplains (see my chapter on Kafue, Zambia).
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Hello, I'm wondering if someone knows what RIN factor should be expected from liver tissue from goats? I'm using the Agilent Bioanalyzer for that.
Thanks a lot!!
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I have not tested RIN (or RQI, which is bassically the same measure of RNA integrity/quality using a experion bioanalyzer from Bio-rad) of goat liver. But I have measured RIN/RQI of bovine liver, I personally do not think there should be big differences among species. What I know from my research is that the RIN/RQI values can be drastically afected by handling and sample procesing. From samples from  bovine liver from healthy animals obtained via biopsy  and inmediately snap frozen in liquid N and then stored at -80C I got RIN values over 7.5. However, from bovine liver samples subjected to in vitro treatment via culture of 20 mg of  liver slices for 2 h and then stored at -80C I got quite low RQI values averaging~4 which was similar to that reported by others for cultured brain tissue.
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I am after some datasets or databases in Europe which shows the variability of nutritive characteristics and mineral concentrations of dairy feeds like the DairyOne in the US.
Appreciated. 
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Hello,
Good question. There are some databases from comercial software for feed formulations which are based on INRA research and also NRC or CNCPS. You can search for NDS or Brill software.
From my experience, minerals from feedstuffs are different from region to region, due to different soils from area of cultivation and also agricultural management of lands (crop rotation, fertilisers), local industry and so on. For example, in SE Europe, I noticed a Selenium deficiency in soil or higher K and Fe C% in the forages.
Hope it helps you.
Kind regards
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How feed flavors will impact in the livestock...especially dairy animals...
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Some flavors, i.e. essential oils (thymol, eugenol, vanilin or limonen), are added in the ratio to maximise DMI, especially during the summer, when DMI decreases and ratio nutrients C% must increase.
Adding flavor should be done gradually, in order to reach the balance of their costs (ROI) and milk yield, but do not forget the palatability of the feeds and forages you deal with. Choose flavors which has the lower inclusion  rate, not to impact the whole DM of the ratio (I'm using 1-5g/cow/day).
Other factors that should be taken into consideration before adding the flavor in to cow's ratio: feeding management, feeds and forages management and other feed stuffs available in the market for making the ratio.
Impact, from my experience, is beneficial, resulting increased milk yield due to a correct estimation of DMI intake and a better conversion of feed in nutrients (some flavours have also matrix values for energy and protein). Is cheaper and safer to increase the DMI, rather to increase the nutrients C% which can lead to metabolic disorsers and so on.
Hope it helps.
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Is there a publication where the energy consumption of a conventional dairy barn (natural ventilated) with around 200 dairy cows is listed?
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Dear Ulrich,
Perhaps the report attached below may be helpful to you.  It contains energy consumption data for several dairy barns (free stall and tie stall types) in different sizes (number of dairy cows per farm).  It was useful for me when we did an analysis of greenhouse gas emissions from Ontario (Canada) dairy farms.
At the same time I have added another link where you can download a similar report; however, it is for relatively smaller farm with fewer dairy cows.  Hope these are useful to you.
Cheers.
SJ
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By using above mentioned raw materials I need to formulate good ration for Frisian Jersey dairy cattles  
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Also, you need the nutrient requirements of animal from NRC or ARC then you should determine the nutrient composition of these feed ingredients.By using  computer feed formulation program you can provide best ration.
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In order to estimate metabolisable energy (ME) requirements of lactating buffaloes, three isonitrogenous rations needs to be formulated, each one with 15% variation in  ME. We have two concentrate mixtures with 20% CP, and 2.5 and 2.8 Mcal/kg of ME. Is it mandatory to maintain constant forage:concentrate (F:C) ratio in such studies within the same group, as it was observed difficult to meet desired individual animal requirements without altering F:C ratio.
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Nice work,
I agree with d-r Vieira, best wishes
Also I downloaded for you these two articles .......I know some formulating of ratio in our local Pelagonia farms, but this is for Holstein -Friesian cows, I do not know nothing for bufalloes if it is the same............ good luck
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When working with a dairy cow that is either approaching or at peak lactation, what other feeds should be incorporated into a total mixed ration to meet her energy needs? Also, what could be limiting her highest production level?
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Cows approaching or at peak lactation are in dire need of more energy. Remember that milk fat synthesis requires lots of energy as well. I think what you should consider are;
1-The general nutritional value of the sprouted barley fodder, (mostly the metabolisable energy)
2-High energy supplements, such fat  
We fed HF cows at between 40 and 48 days in milk with canola oil and found that milk production was increased.
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I need to know this relationship.
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Hi Fernando,
Have you thoughta about a possible synergic effect of hypocalcemia and Zinc defficiency?
Regards,
Alfredo. 
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Is there a way to record calf daily gains without expensive and less robust weight scales?
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If calves are on the feeders for 6 to 8 weeks or so, then weekly weights would be sufficient to look at deviations from the average growth curve. In fact, weighing at the beginning and end is probably sufficient, because the day-to-day trends are not particularly useful in terms of managing the calves or interpreting the data. It might be more useful to look at eating bouts and meal size at each bout, because eating time and meal size have been shown to be good measures of stress in dairy cows. One could use video to estimate bouts, but you'd need electronics to estimate meal size.
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In cows’ reproduction, body condition as a good nutrition indicator is used
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A better measure of nutrition level to estimate effects of nutrition on fertility at artificial insemination is change in body condition during during the first 3 weeks postpartum and during the 3 weeks preceding AI. One would like the body condition score during 3 weeks postpartum to be level or slightly increasing for best fertility. If the cows loses body condition score during this period, it impacts fertility adversely at AI 2-3 months later. During 3 weeks before AI, the body condition score should be improving. Glucose may be useful, but it may not be as useful in ruminants as in monogastrics.
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Over the past five years with field data using different protocols TAI in dairy herds realized that ovsynch and pre-synch walks very well with protocols similar to those sold in our country (Brazil) rates. And on farms where there is electronic system for detection of estrus or the use of paint on the tail had higher pregnancy rates than with the use of TAI with P4/E2. 
This is just an observation of field data with approximately 10 000 inseminations. 
Where to p4 / E2 pregnancy rate is 35%, Ovsynch (24%) Pre-Synch (33%) and PGF with estrus detection (40%). Average of all farms assisted 90% of cows are heat after 3 applications of PGF.
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I always like to compare fertility of yearling heifers with that of cows on the same farm, especially if heifers are inseminated after natural estrus. Heifers should have the highest fertility and will provide clues to problems with insemination techniques, semen handling, vaccination programs and general nutrition. Any difference between cows and heifers is then associated with transition cow health, energy balance, heat detection, poor-responders to TAI and similar environmental issues. One can also look at difference between heifers and cows during warm seasons to see the impact of heat stress and its potential carry-over into cooler seasons. Monitoring BCS during 3 weeks after calving can be useful in predicting conception rate at 70-90 days postpartum in lactating cows.
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I quote from 'Natural Cattle care' " Cows who receive the right minerals and are in good health do not need teat dipping. I ran a good herd of milking goats and never used teat dips. The sphincter at the bottom of the teat stays open for about 20 minutes, after milking and for 15 minutes after calf suckling, a douche of cold water will help close.
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Dear colleague!
I think that minerals have indirect influence of subclinical or clinical mastitis!
It is better to pay attention on the ratio proteins: carbonhydrates, and BUN and MUN level, always high urea level influence on the immune system,  as well as on udder health and SCC;
Best, Vesna
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For a project we're planning to grow different algae-species to add as a food-supplement to the (formulated) milk for calves. What species would you choose and why?
Thank you in advance for all you tips and ideas.
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please look you in attach file 
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Trichantera is a shrub high in protein and is given in varying amounts to growing calves and adult dairy animals.
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Thank you for your response Rolando.  We have the Trichantera gigantea on our station and I am hoping to use the leaves from the shrub to feed calves after weaning.  With respect to the amount to feed to the calves will be critical.   
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We usually follow the NRC system, but it might not be suitable within our local conditions.
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You can read book:
TROPICAL DAIRY FARMING
published by Landlinks Press
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Thank you in advance for your replies.
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Rumen microbes perform two extremely important tasks - fermenting the ingested diet into volatile fatty acids which the host can utilize as an energy source, and utilizing some of the feed components to synthesis and grow, providing the host with a source of protein. Rumen bacteria are required to survive in a "soup" of fermenting material, fluids, gases, and competing microbes, including predatious protozoa. Like any other "jungle", genetic diversity and advancement is essential for a species survival therefore, genetic transfer enables this. Also, gene transfer can result in a more efficient microbial mass in the rumen, improving fermentation while potentially leading to the abatement of inefficient greenhouse gases such as methane.
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Dairy cattle need both macro and micro nutrients to be optimal in productivity.
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Multifactorial nature.
1. Genetics (modern dairy breeds have decreasing fertility)
2. Management (including oestrus detection, transition cow management, housing system, etc)
3. Nutrition (deficiencies and suboptimal levels, but also imbalance between main nutrients [carbohydrate, protein fat, minerals, vitamins])
4. Disorders (genital tract, generalised conditions, e.g. infectious, lameness, mastitis)
5. Level of production (inversely proportional relationship)
It is a common mistake to concentrate on only a single factor (like deficiency of microminerals) and not addressing the real situation. A holistic approach to the problem is required. Fertility is one of the production disorders. Therefore, the approach to a fertility problem should be addressed following the principles of herd health management.
Hope this helps.
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Most methods involve GC determination of fatty acids in milk. I have some information about precolumn derivatization on HPLC. At this moment I would like to see a specific work and experience of a researcher.
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Dear Sasa
Analysis of common fatty acids (with one straight chain and one acid group) is usually carried out by GLC but in special cases it may be necessary to process HPLC separations.A reversed-phased HPLC separation of underivatized fatty acids from oils and animal tissues was proposed after low temperature saponification (Nishiyama-Naruke A et al., Anal Lett 1998, 31, 2565). A simple HPLC system allowing the separation of short, medium, and long chain fatty acids has been described (Kroumova AB et al., Anal Biochem 1995, 225, 270). A more sophisticated and precise method combining HPLC and mass spectrometry was developed to measure short-chain fatty acids in blood (Van Eijk H et al., J Chromatogr B 2009, 877, 719).
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For the kharif crop, it is necessary to use furadon on maize for controlling borers at 25 days after sowing the seed. Where integrated dairy and corn production farming is practiced, it is a very burning issue and hard to say exactly.
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Spot application of furadon can result in some serious residue problems that could affect cattle. Normally, the waiting period for most of the granular insecticides, in general, is 32-45 days. There sre some good options available to manage this pest in corn. One can go for inundative releases of Trichogramma chilonis after 2-3 weeks of emergence. if that is not feasible, foliar application of deltamethrin can be used after 15-17 days after emergence. But a waiting period of at least two weeks is very important.
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Literature says 8 mm is sufficient but when we fed TMR to dairy buffaloes with a roughage particle size of 20 mm, a better milk yield was observed in buffaloes fed TMR with 20 mm particle size. Possible reason?
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8mm is far to small, you need effective fibre to be at least 20mm which will encourage ruminantion and cud chewing - cud chewing enhances saliva production and buffering of the rumen which in turn prevents acidosis and promotes fibre digestion. watch diet NDF levels as well - 20% of NDF from forae and 35% NDF in total