Science topic

DSM - Science topic

Categorical classification of MENTAL DISORDERS based on criteria sets with defining features. It is produced by the American Psychiatric Association. (DSM-IV, page xxii)
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Initially, the individual appeared to be intellectually gifted and possibly on the autism spectrum. It has become evident that she whispers for an unknown reason, while others are around, without apparent realization that she is doing it. Occasionally, she will hold her hand in front of her mouth while whispering. It may be a sentence or a 30 second speech. One time, she spoke normally to a companion, then whispered, then spoke normally again. It happens more often when she is tired. Are you familiar with this phenomena? Are there any thoughts other than schizophrenia? Thank you.
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Hai. I observed this with a normal person when he is in difficulty and trying to solve a problem, he whispers to himself like talking to his inner-self in a "loud" for an answer.
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Hi Researcher,
I am currently working on my master's thesis on Digital Soil Mapping – Soil Organic Carbon. Since I am using soil profiles from a legacy soil survey as training data, I am facing some confusion. The attributes meet my needs, but the temporal dimension poses a challenge.
I have over 700 soil profile points, covering the period from 1980 to 2005. I only want to use this data for the year 2003, but after filtering, the density of the profile points is low, covering only 50+ points.
I would like to ask, how can I resolve this issue? Can I use the entire sample set as training data despite the temporal differences? If so, is there a need for special treatment of this temporal attribute, such as harmonizing or aggregating SOC values up to 2003?
Thank you.
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It seems quite complicated, so it is difficult to find resolution. Anyway, I suggest to:
1. Prepare at least 2 versions of DSM, based on legacy data:
- with the whole dataset of 700 soil profiles (or about 600+ profiles up to year 2002;
- with dataset of 50+ points from the years 2003+,
and than compare their agreement.
2. Be aware, that the soil changes, and the content of SOC could be different at moment of obtaining data and this changes depend on land use (arable, pastures, forest?), fertilization, particularly manuring, and other factors. For this reason the assessment of current status of SC in the points of obtaining legacy data would be very important, if possible.
3. Be aware on some inaccuracies which could have appeared during soil mapping, such as less exact position of soil sampling points or profiles (it was difficult to record precisely geographic coordinates GPS receivers for earlier studies), map generalization, perhaps other methods for determination of SOC?
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We are looking for a "fast" (màx. 1 hour) diagnostic tool for mental health disorders in children and adolescence. We are developing a project with children and adolescences from several hospitals and we need a unique, valid, easy and fast tool to make a diagnosis (following DSM-V or ICD10). Which is the most used tool in research? Can anyone help us? Many thanks in advance.
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CBCL and YSR, still is more a screening tool
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May I ask for some assistance regarding the sporulation of Bacillus subtilis bacteria? I am currently looking for a commercial culture medium that is suitable for this purpose, and have come across the DSM medium in my research. However, I have found that different articles suggest varying amounts of ions to be added to the medium, and I am unsure which ionic form to use and in what quantity. Any guidance or information on this matter would be greatly appreciated. Thank you.
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for sporulation try with Mncl2 Mgcl2
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I am encountering issues with building DSM/POPE/POPC/Cholesterol membrane . My job id (if I read my files correctly) is 8388718807. The error states: "CHARMM was terminated abnormally. Please check the output. The bilayer generation is stoped to prevent infinite loop. Please refresh the browser to restart the bilayer generation with different random seed." Could anyone suggest what can be the reason?
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I found this thread helpful. I was trying DPPC w/ Cholesterol and it didn't work with the default surface area in step 2. This is the column that has 40.0 default for Cholesterol and 63.0 for DPPC but because the surface area is probably larger than 63.0 for DPPC, I increased to 75.0 and it worked.
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Dear RG community,
We have a X. fastidiosa strain in the lab acquired from DSMZ (DSM 103417) and it is being really hard to grow it in the lab.
We are not able to achieve the growth rates described in the literature when using the same media.
We have been using several growth media and gelling agents (BCYE, PW, GYE, agar, gel-rite).
Can you share your insight on how to deal with this culture?
Thank you so much for your help,
BR
Carla Pinheiro
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Xylella fastidiosa is a plant pathogenic bacterium, not typically cultured for general purposes. However, if necessary, grow it in a sterile culture medium containing essential nutrients, maintain a pH around 7, and incubate at 28°C. Use appropriate safety precautions due to its potential impact on plants.
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I am processing World View 3 Satellite Imagery and get following error message while creating DSM or orthomosaic. Any suggestion ?
Generating Digital Surface Model...
Start Time : Friday, July 21,2023 7:42:49 PM
ERROR 003048: The input folder does not contain LAS files.
Failed to execute (InterpolateFromPointCloud).
Failed at Friday, July 21, 2023 7:42:51 PM (Elapsed Time: 2.04 seconds)
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The error message you encountered indicates that the input folder you are using does not contain LAS (LiDAR) files, which are necessary for generating a Digital Surface Model (DSM) or orthomosaic. This suggests that the processing software is expecting LAS files as input, but it is not finding them in the specified folder.
World View 3 Satellite Imagery is typically not provided in the form of LAS files, as LiDAR data is different from satellite imagery. Therefore, you need to ensure that you are using the correct data and processing workflow for your specific task.
Here are some steps you can take to resolve the issue:
Verify Data: Double-check the data you have and ensure that it is indeed World View 3 satellite imagery, not LiDAR data. If you intended to process satellite imagery, make sure you have the correct data format.
Confirm Software Settings: Review the settings in your processing software to ensure that you have selected the appropriate data format for the input files. If you are processing satellite imagery, choose the correct file format for that data.
File Locations: Check if the input folder specified in the processing software contains the correct files. Ensure that the file paths are correctly specified in the software.
Supported Formats: Verify that your processing software supports the file format of your World View 3 satellite imagery. Most commonly, satellite imagery is provided in formats like GeoTIFF, JPEG, or other raster formats.
Consult Documentation: Refer to the documentation or user guide of your processing software to understand the correct workflow for handling World View 3 satellite imagery and generating DSM or orthomosaic.
Seek Support: If you are still encountering issues, reach out to the support resources of your processing software or the provider of the World View 3 satellite imagery for assistance.
By following these steps and ensuring that you have the correct data and processing settings, you should be able to successfully generate DSM or orthomosaic from World View 3 satellite imagery.
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I need to find main directions of wind flow in a city. Data of DEM and DSM are available. I am looking for a simple method to find the main directions of wind flow in a city to calculate air pollution risk. I appreciate any answer.
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I suggest you read the paper “Wind energy potential assessment based on wind speed, its direction and power data prepared by Zhiming Wang & Weimin Liu and published by Scientific Report 2021.
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And has it been updated for DSM-5? If updated, where could I access it/download it/purchase it?
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What is Cidi-Sam?
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In DSM trail frequency is taken and then bisection technique is used to calculate the root in certain range of the error. This technique is good however there are many drawback, high time computation etc. Please suggest other numerical technique through which we can address this type of problem.
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The book "Numerical Recipies" contains an algorithm the authors call RFSAFE (I believe) that combines the speed of Newton Raphson with the safety of binary search. As long as you isolate the zero your looking for between two numbers (i.e. bounds) just as required by binary search, you will compute that zero using RFSAFE. Note that there needs to be just one zero that lies between the bounds.
However, in agreement with Mr. Benton's answer, you should check your implementation of binary search first. I recommend trying RFSAFE only if your implementation of binary search is correct and it's still too slow due to the number of instances of its use.
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Hello everyone, I am culturing the Phosphorus bacillus (Photobacterium phosphoreum
DSM 15556). After culture, the fluorescens can be detected via luminometer (over 3 million), but can not observe the fluorescens via eyes (baed on the website images, a very bright blue fluorscens can be observed from culture medium). So, how I should do to observe the fluorescens? Thanks in advance.
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For many of the beneficial bacteria bacteria there fluorescence is not visible in the visiible light spectrum but there fluorsecence is found and detected under ultraviot light exposures.
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I don't find the number of disorders in DSM IV, DSM IV-TR, DSM 5 and DSM 5- TR (there is a lot of confusion). It would be a great Help to clarify this confusion.
Thank you.
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It is useful to remember two things. The first is that in the US, your insurer requires a diagnosis before they will pay for your treatment. For this reason, DSM tends to have a wide coverage. These are not diseases so much as categories for insurance reimbursement.
The second is that every diagnosis in DSM is fought over. Researchers who disagree do research, bring data to the table, argue their case. For example, the original DSM diagnosis of bulimia did not include any psychological symptoms, and resulted in a slew of research that resulted in significant changes to the next version. Likewise, the inclusion of borderline personality disorder (which required some hard-won battles!) resulted in a surge of interest in developing therapies and refining the diagnostic criteria. And the current DSM is still struggling to get useful criteria for personality disorders – far from being settled, the controversy continues!
So DSM is a work in progress, always. It will always arouse controversy, but I regard this as a vital function.
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Deep Soil Mixing requires a certain liquidity for injecting into soil. What is the minimum requirement of it?
LRF slag & fly ash will be used as Binder instead of using cement. Sodium Silicate, Sodium Hydroxide will be used as activator.
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Dear Tanjii,
depending on the equipment you use the viscosity of the slurry can vary but of course must be very liquid and fluid.
Check about jet grouting method where you pump at very high pressure the slurry (few seconds on Marsh cone test) in the ground till 40-50 meters in depth. In this case the standard product used are Portland based. I think you will find there all the specifications.
If you use less powerful system you can use more creamy consistency but still with low Marsh cone time, to be pumped in the ground through thin pipes using till 5-6 Bar of air pressure, at 3-4 m of depth.
Using Geopolymers I had good results using both equipments adjusting the fluidity using extra water (quite low however because I designed very fluid GP binder).
But I think you are working with AAM alkali activated materials that have completely different balancing. Because GP and AAM aren’t the same and also they have very different way to harden.
For this application I suggest to check about your precursor because specially fly ash is polluted material with lot of heavy metals and often even lead so pumping into the ground couldn’t be the best solution (I’m thinking about water source ).
But I don’t know your project.
Best regards
Alex Reggiani
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Is there any platform that contains DSM data?
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Do you mean the Diagnostic and Statistical Manual of the American Psychiatric Association? And if so are you looking for diagnostic data on patients or what? No-one has answered this because it's not at all clear what you want.
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Hello friends
I work on the drying up of forest trees. My data source is RGB images of drones. I processed the images in Agisoft software. Unfortunately, the drought of the trees in the digital model of the canopy surface is not well made.
I did not produce textures in image processing. Is this step necessary to prepare DSM or DEM?
Do you have any comments or suggestions for improving the digital level model?
(The altitude of the drone is 100 meters and the overlap is 80%).
Cheers
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Thank you bro
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Demand Side Management is one of the key tools in smart grids. It is aimed to control and manipulate of the demand by DSM. Demand response programs in DSM provide an option to control and manage the demands via incentives or electricity price. Which performance indices are used in DSM and demand response programs in order to manage the demand? Also, which performance indices are used in DSM in order to evaluate the effectiveness of the DSM?
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Thank you for your answer Dr. Qamar Ul Islam . But I want to learn and discuss the metrics or indices that can be used in evaluation of the DSM performance.
Best regards,
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Hi
I recently took pictures at a height of 100 meters above the ground. But when processing with Agisoft software, part of my study area is lower than other areas (the study area is flat). What could be the reason?
Thanks
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The determination of flight conditions varies based on the UAV model, flight application, and the
size of the imaging area; thus, preparing a manual for flight conditions is extremely dicult. There
are many issues to consider for UAV utilization, such as various flight conditions and di erences in
tree species. Therefore, in order to utilize UAV in forest management, it is important to accumulate
basic data related to estimates of tree height and volume using UAV, for verification of accuracy.
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I need this information for my project
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In DSM 5 there is a specifier for Major Depressive Disorder, "with peripartum onset". This concerns major depressive episodes that start during pregnancy or within the four weeks following delivery. The four week postpartum specifier has been a source of considerable discussion in the literature however that's what is in DSM 5.
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I am looking for a tutorial for an Excel plugin designed by MIT University to draw a Design Structural Matrix(DSM). This code based on C can be run as an add-on in Office Excel software that allows the user to model up to 100 parts. I will be thankful if you have any training or information on how to work with this plugin, help me.
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No, but if you tell me I will.
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I used to work with software DSMMatrix v1.8 to draw the Design Structural Matrix(DSM) in my projects, but now I am looking for better and more powerful software to draw the design structure matrix. The main problem is the limited part entry in DSMMatrix v1.8.
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Which method is being used for interpolation in DSM generation using Pix4D Mapper, Metashape and Inpho UASMaster?
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Most software package uses IDW and TIN based interpolation.
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Are most people, who score high on neuroticism, score high on covert/ vulnerable/ hypersensitive narcissism, too?
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Narcissism and neuroticism are not the same. The former is a personality disorder and from that often the environment suffers more than the person him/her self. A neurotic person suffers more than the environment from his/her symptoms. There is also a difference in reality perception.
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Hi,
I am hoping someone can inform me as to how the SCID-5-CV is scored? I have been looking online but can't find too much without purchasing handbooks from the DSM.
I am looking at hypomania symptoms in patients with bipolar disorder and want to know if I can use the SCID to measure hypomania symptoms between time points (e.g. at baseline, 6 months, 12 months etc). As such, I want to be able to determine episodes, duration, severity etc. from one time point to the next. Moreover, I would like this to be in a way that is quantifiable, for statistical analysis.
Can the SCID-5-CV allow for this? Or alternatively, is there a more appropriate measure?
Thanks,
Emily.
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I am extracting building height values from DSM polygons with building shapefiles
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If you load the polygons into a file geodatabase it will add a perimeter field. Or just add a field to the shapefile caller "Perim" and then right-click and "Calculate Geometry > Perimeter"
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I had converted building DSM to polygon and trying to extract building height values along boundary of polygon. I am using ArcGIS desktop and pro.
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It sounds like you're trying to extract raster data to a polygon file. If that's the case, the extraction tools in ArcGIS should do the trick: https://desktop.arcgis.com/en/arcmap/10.3/tools/spatial-analyst-toolbox/extract-by-polygon.htm
My guess is that this particular tool is the one that you're looking for: https://desktop.arcgis.com/en/arcmap/10.3/tools/spatial-analyst-toolbox/extract-by-polygon.htm
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In order to fulfill criteria for Somatic symtom disorder (DSM 5) a patient needs to be preoccupied with symtoms or their health. Reasonably a symtom like pain does make you preoccupied to some degree. So how have researchers tried to operationalize "preoccupation"?
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As a logical, normal and adaptive worry or affliction and not as an irrational, hysteroid or hypochondriac cognitive-emotional nanifestation ... moreover, I would classify it as a healthy response and, I reiterate, ADAPTIVE
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DSM V seems to be much better when compared to ICD 10 for clinicians. ICD 11 has a number of changes. It'll be a great guideline once out for use. How do you compare the two guidelines, ICD 11 and DSM V?
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Yes Randy. I too really liked multi axial system of DSM IV. Its very convenient when formulating a case. DSM V diagnostic trees are bit confusing sometimes. ICD 11 seems to be much better.
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Quarantine fatigue is not uncommon during this period. Can we include it under a disorder category in ICD or DSM? Is it an adjustment disorder?
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Features of Cabin fever are very similar to that of quarantine fatigue. The syndrome comes in different disguises. I have seen a number of patients presenting with symptoms of depression and anxiety.
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I've used Agisoft Metashape, and the weeding I.e. the removal of trees and the vegetation is coumbersome. So I need another software that will give a better model. I'm working on Pix4D though. Thanks
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Following the suggestion of Saheed Olaniyi about using the point cloud, I would recommend the open source solution CloudCompare. You can classify your point cloud and create a DEM using its plugins.
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Etzel is a very distinguished figure in Psychology,DSM 5 Dissociative Disorders,and a pioneering investigator and leader and editor of empirical Psi research.
Please consider inviting him to participate in your project .He is on Research gate
Ian Wickramasekera,PhD
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yes, thank you!
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I have a Bacillus cereus strain that never sporulates in DSM (Difco Sporulation Media) and it does not usually sporulate either in LB media, but sometimes when I recover an O/N culture and I heat it at 80ºC 10min, I see several colonies that correspond to spores.
My question is... why sometimes I recover spores in LB media and other times none?
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I think maybe you need pay attention to the initial concentration of Bacillus cereus you inoculated and the culture time of Bacillus cereus. And sporulation is a normal stage of many spore bacteria.
Best wishes.
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We use polyamide 6 / formic acid solutions for electrospinning in the lab. When the prepared dope is aged over 3-5 days, it gets to a gel like state with heavy precipitation. When left longer, it almost completely solidifies. This happens in closed volumes, i.e. in jars with tightly closed lids, so we left out the possibility of solvent evaporation. When using different types and brands(BASF & DSM) of PA6, the curing time changes.
Do you know any mechanism that would cause PA6/FA solution to cure/cross-link/precipitate?
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Abdelkader BOUAZIZ Thanks for the reply, my latest finding is that the same dope solution diluted in benzyl alcohol cures much faster, like in 18 hours. Does this follow with your guess? Also, is there any inhibitor to keep this reaction happenning?
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(I was inspired by a post I just saw)
What books are recommended for newer Master's level clinicians (or perhaps PhD)?
So far I've got:
1) The DSM-V (APA)
2)101 Solution-Focused Questions
3) Theories in Integrated Practices
4) Psychopathology
Please feel free to share books, articles, tips and beyond.
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Viktor E. Frankl: Man's search for meaning has profoundly impacted my way of thinking. As a general rule textbooks are rather old and review articles are more
fresh and useful. This I have discovered. It is impossible to know what you are most interested in. I can attach some papers of my logotherapeutic interest:
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I am undertaking a review of depression in a specific population and the majority of the research studies included use a questionnaire to assess for depression symptoms. When writing up the review is it acceptable in a academic paper to describe the “prevalence” of depressive symptom? are there examples of papers where the authors defend the use of the term “prevalence” when discussing symptoms of depression. Are there definitions of “prevalence“ that can be used to justify using the term “prevalence” for symptoms of depression and not a clinical diagnosis as per the DSM V?
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In general, I concur with the responses from McColloch, Philpott, and Repisti. McCulloch's last sentence, " I personally am happy to see the term used as long as I understand what is being measured in what population. ", is what I would like to focus on. In diagnosing using the DSM-5, we use the terms prevalence and incidence as related to individuals receiving a particular diagnosis with prevalence being the number of individuals receiving that diagnosis at a particular point in time (ex. today or this day) and incidence being the number of NEW cases of a disorder within a given point in time (ex. new cases within the past year). However, prevalence, as stated by the above researchers, does not simply refer to a diagnosis. Like McCulloch, I would happily accept the use of the term prevalence if the research thoroughly describes how prevalence is being used.
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An Unmanned Aerial Veichle (UAV) based survey is being conducted for an area which is more or less a flat terrain type. The post processing of the UAV images results into a digital elevation map which shows the height of buildings along with the surrounding agricultural areas.
So, could anyone please suggest the procedure for separation of the surface heights from the raster file to create the actual elevation of the region?
Thank you in advance
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Hi Saroj Kumar Dash, you could work directly with UAV point clouds and exporting them in CloudCompare, after that try to get the DTM using the CSF plugin.
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I am designing a roof trapezoidal roof profile with both longitudinal and transverse stiffeners. I am looking for related solved problems/design guidelines particular to Direct Strength Method as I am new to this method.
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Refer this it may be useful
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To the microbiologists out there:
I'd like to measure total bacterial/fungal load in clinical samples by qPCR with published primers+ probes targeting the bacterial 16S/fungal 18SRNA gene. For standardization I'm going to create a plasmid standard curves with the PCR product of DNA from pure cultures
For the determination of the molecular weight of one copy, I need the sequence or at least exact length of the area between the primers. However, I cannot find the genome of the strains I'm working with, might be due to lack of knowledge about nomenclature or because it's not published so far?.
--> Do different strains of e.g. E coli or other microorganisms differ in sequence in the 16S/18S rDNA region? Or could I just use the sequence of another strain accessible via ncbi? Or do I necessarily have to sequence if I cannot find the sequence in the internet?
I work with:
- common E. coli, K12, DH5alpha, thyA-
Thanks a lot!
Sarah
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Hi Sarah,
Please take also into account that the number of copies of 16S rRNA on the genome can vary across bacteria from different species (1 to 15). If you will experimentally contaminate your samples with your E. coli strain of interest, this difference is of course not relevant, but it can lead to misleading results if you have more complex microbial communities.
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The focus would be to potentially use DSM to explore the dependencies and interactions between subsystems, where a subsystem may have several technology options available for Product Design/Development (PD).
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Mohsen - I was asking a question about SBD and not an answer on the human condition. I’m an engineer and not a designer. Good luck with your research.
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Since you set up the research project, have you found that your culture ZM1 degrades polystyrene?
In general, you to deposit the culture to a culture collection center such as ATCC, DSM before you publish your results.
Thanks!
Wei-Min Wu
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If you can to west coast, you are welcome to visit our lab.
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Ground improvement methods such as Deep Soil Mixing (DSM) and Jet Grouting (JG) are used for liquefaction mitigation. They reinforce the ground, thereby reducing the cyclic stress ratios (CSRs) induced in the soil. Boulanger et al. CSR reduction factor curves are developed for soil-cement "grid" reinforcement.
I want to use discrete soil-cement "columns" for liquefaction mitigation. Is there any design criteria for soil-cement columns?
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What is the significance of considering discrete soil-element columns? In practice, regular spacing is usually used. If necessary, the spacing may be varied to mitigate the liquefaction susceptibility of the specified cites.
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Hi. I am a MPhil student at Amity University, Noida, India. I am studying the changes in prevalence rates in child onset schizophrenia after the DSM -5 increased the criteria for diagnosis from "1 to 2".
If you have any researches that have studied this, please share.
Thank you.
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Dear Shreya,
You might consider reading the following articles by Tandon et al.
"Data suggest that changes from DSM-IV to DSM-5 in the definition of criterion A symptoms should have little impact on caseness of schizophrenia, with less than 2% of patients with DSM-IV schizophrenia not meeting DSM-5 criteria because of these changes. Our findings are similar to those of the two other studies that have investigated this issue (Peralta and Cuesta, 1999, Allardyce et al., 2007)... the explicit conceptualization of schizophrenia as a psychotic disorder and a requirement that psychotic pathology in the form of delusions, hallucinations, or disorganized speech be present in order for it to be diagnosed... should have little influence on the prevalence of schizophrenia as over 96% of individuals diagnosed with DSM-IV schizophrenia were found to have at least one of these “positive” symptoms."
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I have referenced to some papers which extracted DEM and DSM based on LIDAR data. Then HRSI was fused with Lidar data. is this method effective? Are there other effective methods?
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If you are looking for a software, FME has an efficien attribute fusion pipeline. Else, the paper may provide you with additional methodology bulletpoints
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To create a DSM model from DEM points based on Drone Images
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I'm agree with Dr Riedel. In addition you can follow this link:
I hope this will help you
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I need the fors and the against the elimination n of the bereavement exclusion
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Since 1980, the DSM-III and its various iterations through the DSM-IV-TR have systematically excluded individuals from the diagnosis of major depressive disorder if symptoms began within months after the death of a loved one (2 months in DSM-IV), unless the depressive syndrome was 'severely' impairing and/or accompanied by specific features. This criterion became known as the 'bereavement exclusion'. No other adverse life events were noted to negate the diagnosis of major depressive disorder if all other symptomatic, duration, severity and distress/impairment criteria were met. However, studies since the inception of the bereavement exclusion have shown that depressive syndromes occurring after bereavement share many of the same features as other, non-bereavement related depressions, tend to be chronic and/or recurrent if left untreated, interfere with the resolution of grief, and respond to treatment. Furthermore, the bereavement exclusion has had the unintended consequence of suggesting that grief should end in only 2 months, or that grief and major depressive disorder cannot co-occur. To prevent the denial of diagnosis and the consideration of sometimes much needed care, even after bereavement or other significant losses, the DSM-5 no longer contains the bereavement exclusion. Instead, the DSM-5 now permits the diagnosis of major depressive disorder after and during bereavement and includes a note and a comprehensive footnote in the major depressive episode criteria set to guide clinicians in making the diagnosis in this context. The decision to make this change was widely and publically debated and remains controversial. This article reports on the rationale for this decision and the way the DSM-5 now addresses the challenges of diagnosing major depressive disorder in the context of someone grieving the loss of a loved one.
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The authors of DSM-5 chapter on intellectual disability made a significant error (typo!?) in reporting the prevalence of intellectual disability. Actually, subheading PREVALENCE in DSM 5 consists of only two sentences:
Intellectual disability has an overall general population prevalence of approximately 1% and prevalence rates vary by age. Prevalence for severe intellectual disability is approximately 6 per 1000.
So, it means that there are 10 people with intellectual disability (all levels of ID) on 1000 people. According to DSM-5, 6 out of these 10 people are people with severe intellectual disability. However, most studies point that out of all people with ID, the majority of them (85%) have mild ID. I think this needs to be corrected!
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I believe that the prevalence of ID in the overall population is around 1%, and the prevalence of severe ID within the population of people with an intellectual disability is 6 in 1000.
That is, 6 in 1000 individuals with an ID have a severe ID.
I have read that about 85% of individuals with ID have a mild ID, about 10% have a moderate ID, and about 10-15% have a moderate ID, and a small percentage (under 1%), have a severe ID. This fits with what is written in DSM-5.
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Dear all,
I'm looking for DTM or DSM with geometrical resolution below 10 meters for pixel of Dead Sea region (Israel). Priority for free information sources but also ready to pay for any appropriate data.
I heard that Spot-5 DTM should be free to download but I can't find the sources.
If you know any sources for free DTM or by payment, please share.
Thanks in advance
Semion
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Dear all
I have a dense point cloud (.Las) which is consist of 150 million points and I want to create a Raster file (i.e., DSM) from it. The question is: which software can do it? I used the LASTools, ArcGIS and TerraSolid, however; none of them could handle such a big file.
Thank you in advance,
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I would recommend the LAStools software distributed by Rapidlasso (free and commercial versions) that is specially designed to work with large clouds of 3D points.
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I am having WorldView-2 and WorldView-3 imagery (includes SWIR bands) of dense urban areas. I want to extract building footprints (2D and 3D) of very complex buildings. For 3D, I have DSM.
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A multilayer perceptron (MLP) is a class of feedforward artificial neural network. An MLP consists of at least three layers of nodes. In input instead of pixel's value I suggest to use a aggregate pixel's value that is a patch. For example the mean of patch of 100x100 pixel so you taking into accunt adjacent pixel, shape and so on. Patch dimension is function of image sizew and what do you want to classify
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Hi, everyone currently I am working on optimization for demand side management (DSM) problems. So, I want to implement my proposed DSM scheme in the micro-grid environment. For that, I need standard micro-grid system data. Please suggest me how can I get that standard system data or any sample data. I am grateful to you if you help in this.
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thank you Lokeshgupta Bhamidi , you can use simulation data .
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Dear Researchers,
1. The soil improvement technique Deep Soil Mixing (DSM) is very common presently. How do we model the DSM columns of a given site in PLAXIS?
2. While liquefaction risk is present after doing analysis in Plaxis 2D, what measures can be taken to prevent liquefaction ? and can those measures be modelled/applied in Plaxis 2D?
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You can convert from 3D to 2D using an averaging technique mentioned in ppt for numerical simulation of DEEP MIXED column using plaxis 2d
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Hi all,
I just received a LiDAR dataset consisting in five different folders. I am reporting the label of those folder in case there is a standard nomenclature: c_1, c_2, c_3 ,c_4 and c_5.
All the datasets cover the same area. Provided that c_1 represents the DTM (floating) and c_2 represents the DSM (floating), the issue I have is identifying the remaining three layers, and specifically:
- c_3 has a 'unsigned integer' pixel type and a classification range from 0 to 255, maybe they are co-surface point groups?
- c_4 has a 'unsigned integer' pixel type with just 4 groups under the value 'Area'
-c_5 is almost identical to c_1 (the DTM) with slightly different values.
Any help on the meaning of all these layers??
Thanks anyone in advance.
Alessandro
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Hello-
My recommendation would be to contact the vendor for an explanation. There are no standard folder naming conventions for LiDAR data. The standards referenced by the ASPRS website are in regards to the las files, not folder names. I also wouldn't assume that c1 and c2 are the DTM and DSM only because there's no standard for folder naming. Of course all of this could be resolved if there was metadata written, but I'm assuming this doesn't exist either.
Good luck
-al
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How to perform demand side management in smart grid using agents?
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Hi, I am curently doing my research on residential demand side management (DSM). I want to analyse the DSM concept with renewable sources. For that I required sample data of residential solar PV power generation or wind turbine power generation. I am very thankful for anybody to help in this.
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The legacy version of the HOMER energy https://users.homerenergy.com/pages/homer_legacy contains some useful case studies for you. You may also build a model for a small system (like a household) using the HOMER QuickStart http://quickstart.homerenergy.com/#/intro
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Satellite image orthorectification of VHRS data(GSD<0.5m) is challenging in undulating terrain especially hilly areas. And it is known that the DSM available like Alosworl3D(30m posting), Carto DEM(10m posting), ACE2DEM(90m posting), etc., though may be accurate are not directly usable because the hill tops and valleys may not accurately register with the GCPs available.
In such scenario, do we need to go for LIDAR based elevation values which is a costly affair? Or is it suggested to use stereo pair which is also a costly affair?
What are the methods in practice that are in use and are found to be cost effective and accurate inorder to get orthorectified imagery that could be used for urban utility mapping using VHRS satellite imagery(GSD<0.5cm)?
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Hello!
I think you need to work from whole to part, instead of part to whole. In ERDAS imagine mark all GCP and supply respective coordinates, while rectifying select elevation source from DEM either cartsat or dem.
"though may be accurate are not directly usable because the hill tops and valleys may not accurately register with the GCPs available." I dont think it has much effect if you use 90m, 30m, or 10 m DEM. Since the smallest measurable unit in RS is pixel. If you have GCP whose precision is +_ 5 cm, that will fall any where on 10 m pixel.
In such scenario, do we need to go for LIDAR based elevation values which is a costly affair? Or is it suggested to use stereo pair which is also a costly affair?
As you said above methods are costly and doesn't improve your accuracy very much in-terms of orthorectification. 'Economy of the accuracy' is crucial here.
regards
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I would like to know if the MINI neuropsychiatric interview for DSM V is available in Spanish, because we would like to use it in a RCT we are actually carrying out.
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Thanks for your answer. We are looking for a brief diagnostic psychiatric interview for our study, that`s why I was looking for more information about the MINI International Neuropsychiatric Interview.
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Looking for references on research on various methods (DSM,etc) for interface management of product interfaces during design and development. I am particularly looking for methods used in automotive industry. The interest is more on the system / sub-subsystem / elemental level interfaces rather than organizational. 
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I just saw your question. It is probably too late, but I the following papers might be of some interest to you.
1.       New directions and new tools for interface management
2.      Interface management of subsea field development
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Dear All
Practically, in Direct load control (DLC) demand response programs, the utility has full control over the registered loads? The consumer-side has no control at all?
Thank you
Sincerely,
Ahmad?
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Dear Ahmad
Registered loads include, typically, pool pumps, air conditioners and electric hot water systems. And yes, the utility has full control over these loads. Instead, the consumers are awarded by a way of financial incentive such as recurring annual payment. The consumer may control the local registered loads in a way that the critical peak is not increased, e.g. the consumer may turn off and/or turn down an air-conditioner which is registered in DLC program. Likewise, as Labib said, the consumer control cannot over write the utility control.
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I am planning a study where I am comparing categorical vs. dimensional predictors of success in therapy. I am using a dimensional measurement tool to predict treatment outcome and am unsure how to operationalize DSM diagnoses as the other predictor.
How might I do this? 
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Whichever method you ultimately choose will have its imperfections, so a step forward might be to acknowledge this dilemma and justify the method you've chosen and how it suits your research purposes but not others'. 
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We have created high resolution DSM using LPS software but not getting the surface smooth while checking in vertical profile graph. It is showing so much undulation, even tried lot of method to smooth the surface but still not getting satisfactory output. 
I have gone through many journal, many experts are saying that it can possible to generate 2 meter DEM from high resolution stereo pair image. But in my case, undulation is coming in water body, road, railway track every where...
Please suggest a software or process to generate accurate DSM without undulation...For railway track simulation, undulation is not at all acceptable...
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Hello everyone, I am currently doing some research about DSM approaches. It would be very interesting to find some studies which compared the energy savings of traditional refurbishment alternatives in buildings (increasing wall insulation, changing glazing elements, ventilation,..) with the energy savings of implementing DSM or home automations such as shading controls or smart lighting, thermostats, appliances, plugs or meters in the same buildings. So far I’ve not been able to find such a study, so any help would be much appreciated. Thank you in advance!
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Dear Tobi, dear Edwin,
thank you so much for your answers, they have been very helpful for my research!  
Best regards,
Laura.
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We're looking for it several times and still there's no result related to it. We will be needing it for our research paper. Thank you.
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Did you see this? The link is more of what you seem to be looking for
Looks like things  will change in 2018:
Journal List
World Psychiatry
v.15(3); 2016 Oct
PMC5032513
Logo of worldpsych
World Psychiatry. 2016 Oct; 15(3): 291–292.
Published online 2016 Sep 22. doi: 10.1002/wps.20353
PMCID: PMC5032513
Bodily distress disorder in ICD‐11: problems and prospects
Oye Gureje 1 and Geoffrey M. Reed 2
Classifying the disorders associated with burdensome somatic concerns has been a challenging exercise in psychiatric nosology1. The classifications of these conditions in ICD‐10 and DSM‐IV have not fared much better than earlier attempts2. Even though not exactly identical, these classifications were broadly similar and criticisms of either system are therefore generally applicable to both. Among the most salient criticisms are those relating to their utility in routine clinical practice. These include the rarity of the major categories of the group, both in the community and in general clinical practice, as well as the evidence suggesting poor diagnostic reliability3.
A central feature of the definition of these disorders, that the symptoms are not due to physical or medical causes, has been criticized for being unreliable and for posing a fundamental nosological problem: defining a disorder on the basis of the absence of a feature rather than the presence of a problem4. Labels assigned to burdensome somatic preoccupations that have come to be seen as pejorative create another problem for clinical utility. Some patients object to the term “somatoform”, which they think may imply that their symptoms are of doubtful clinical importance and are “in their heads” or not real. Furthermore, the notion that the symptoms are medically unexplained is often rejected by patients as essentially an issue of detection.
As part of the activities designed to lead to the approval of ICD‐11 by the World Health Assembly in 2018, the World Health Organization, through its International Advisory Group5, constituted the Somatic Distress and Dissociative Disorders Working Group, which, among other tasks, was asked to propose changes to the section on somatoform disorders in ICD‐10. The Working Group has proposed a new and much simplified category of bodily distress disorder, which replaces all of ICD‐10 categories within the group of somatoform disorders (F45.0) and, to a large extent, neurasthenia (F48.0), bringing these together under a single category. The only ICD‐10 somatoform condition excluded from BDD is hypochondriasis (F45.2).
In the proposed new classification, bodily distress disorder is defined as “characterized by the presence of bodily symptoms that are distressing to the individual and excessive attention directed toward the symptoms, which may be manifest by repeated contact with health care providers. If a medical condition is causing or contributing to the symptoms, the degree of attention is clearly excessive in relation to its nature and progression. Excessive attention is not alleviated by appropriate clinical examination and investigations and appropriate reassurance. Bodily symptoms and associated distress are persistent, being present on most days for at least several months, and are associated with significant impairment in personal, family, social, educational, occupational or other important areas of functioning. Typically, the disorder involves multiple bodily symptoms that may vary over time. Occasionally there is a single symptom – usually pain or fatigue – that is associated with the other features of the disorder” (this is the proposed brief definition for bodily distress disorder; for the format of ICD‐11 diagnostic guidelines, see First et al6).
Responding to the same set of criticisms, the DSM‐5 created a new grouping called Somatic Symptom and Related Disorders, in which the prototypic condition is somatic symptom disorder. Even though this diagnosis can be given to a condition with “one or more somatic symptoms”, it nevertheless requires that “excessive thoughts, feelings, or behaviors are related to the somatic symptoms or associated health concerns”. Specifically, for a diagnosis of somatic symptom disorder, at least one of three psychological criteria should be present: health anxiety, disproportionate and persistent concerns about the medical seriousness of the symptoms, and excessive time and energy devoted to the symptoms or health concerns.
In both the proposed bodily distress disorder and somatic symptom disorder, the most fundamental revision has been the abolition of the distinction between medically explained and medically unexplained somatic complaints. On the other hand, there are now specific psychological criteria that need to be fulfilled before the diagnosis can be given. The revised classifications thus address the problem of defining somatoform disorders on the basis of the absence of a feature (a physical or medical cause) by specifying the features that must be present, such as distress and excessive thoughts and behaviors7.
Dropping the criterion of “medically unexplained” is not without its consequences and has been criticized in somatic symptom disorder. It has been argued that patients with medical conditions and with a justifiable reason for somatic complaints may receive an inappropriate psychiatric diagnosis, with the possibility of associated stigma8. The specification in bodily distress disorder that “if a medical condition is causing or contributing to the symptoms, the degree of attention is clearly excessive in relation to its nature and progression” is meant to address this concern.
A single somatic symptom may lead to a diagnosis of bodily distress disorder or somatic symptom disorder. A good justification for this revision is that a single symptom, for example pain, may sometimes be as bothersome as multiple somatic symptoms. However, the point has been made that this lowering of the threshold for the diagnosis may lead to an inappropriate labeling of apparently healthy persons as having a psychological disorder8. This concern is addressed in bodily distress disorder by the requirement that other features, in particular associated psychological features, as well as significant functional impairment, be present before the diagnosis is given. Also, further information is provided in the proposed diagnostic guidelines that seeks to delineate mild bodily distress disorder from normal somatic concerns which may exist in the community and do not require clinical attention.
One of the important differences between the proposed ICD‐11 and the DSM‐5 approaches is the name of the prototype disorder. While the DSM‐5 has retained the word “somatic”, the proposed ICD category has avoided this term altogether. While no label can prevent completely the risk of negative connotations and misinterpretations, a more descriptive label that avoids the term “somatic” might prove more acceptable to both patients and primary care clinicians.
While the DSM‐5 has retained hypochondriasis (or health anxiety) within the cluster of Somatic Symptom and Related Disorders, the current proposal for ICD‐11 has placed hypochondriasis within the grouping of Obsessive‐Compulsive and Related Disorders. The position of DSM‐5 is supported by evidence suggesting a high co‐occurrence of hypochondriasis with somatization disorder as well as shared cognitive perceptual styles between the two conditions. On the other hand, the position of the ICD‐11 Working Group is supported by findings associating repetitive cognition and behaviors as well as task‐related neural activation patterns on brain imaging with hypochondriasis1. Also, there is evidence that, unlike somatization disorders, hypochondriasis responds to some treatments used for obsessive‐compulsive and related disorders9.
The new proposals for bodily distress disorder are being systematically tested in the field studies conducted as part of the ICD revision process. These studies include Internet‐based approaches, in which a large number of clinicians participate through the Global Clinical Practice Network (http://gcp.network), as well field studies conducted in clinical settings. It is hoped that the findings from the field studies will provide opportunities for a further strengthening of the utility and validity of the classification of burdensome somatic concerns in ICD‐11 prior to its approval by the World Health Assembly.
Oye Gureje1, Geoffrey M. Reed2
1WHO Collaborating Centre for Research and Training in Mental Health, Neuroscience and Substance Abuse, Department of Psychiatry, University of Ibadan, Ibadan, Nigeria; 2World Health Organization, Geneva, Switzerland and School of Psychology, Universidad Nacional Autónoma de México, Mexico City, Mexico
O. Gureje is Chair of the WHO Working Group on Somatic Distress and Dissociative Disorders. G.M. Reed is a member of the WHO Secretariat, Department of Mental Health and Substance Abuse. The views expressed herein are those of the authors and, except as specifically indicated, do not represent the official positions of the WHO Working Group, the International Advisory Group for the Revision of ICD‐10 Mental and Behavioural Disorders or the WHO.
Go to:
References
1. Gureje O. Curr Opin Psychiatry 2015;5:345‐9. [PubMed]
2. Mayou R, Kirmayer LJ, Simon G et al. Am J Psychiatry 2005;162:847‐55. [PubMed]
3. Creed F, Gureje O. Int Rev Psychiatry 2012;24:556‐67. [PubMed]
4. Voigt K, Nagel A, Meyer B et al. J Psychosom Res 2010;68:403‐14. [PubMed]
5. International Advisory Group for the Revision of ICD‐10 Mental and Behavioural Disorders . World Psychiatry 2011;10:86‐92. [PubMed]
6. First MB, Reed GM, Saxena S et al. World Psychiatry 2015;14:82‐90. [PubMed]
7. Rief W, Martin A. Annu Rev Clin Psychol 2014;10:339‐67. [PubMed]
8. Frances A. J Nerv Ment Dis 2013;201:530‐1. [PubMed]
9. Greeven A, Van Balkom AJ, Visser S et al. Am J Psychiatry 2007;164:91‐9. [PubMed]
Articles from World Psychiatry are provided here courtesy of The World Psychiatric Association
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Can someone recommend me a validated instrument for proxy rating of mental illness which is including all ICD-10 or DSM main categories?
I am planning to do a psychological autopsy and from what I have read so far in previous studies it seems like the SKID is used quite often for post mortem diagnosis of mental illness. Unfortunately the SKID is not validated for ratings by proxy and many of the interview questions can hardly be answered accurately by people who knew the deceased.
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Thank you for your answer! I reframed my question and will look into that paper as well!
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We're having a research paper and we need scales to measure our variable. We need DSM-V based scales and we haven't found one yet. 
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I am looking for a source to purchase commercial volumes of 1,4-butanediol and adipic acid made from renewable raw materials.
What I found out is that for biobased adipic acid, DSM and Rennovia are working on it, and for biobased BDO, BioAmber and Novamont.
Are there any other suppliers who have reached or will soon reach maturity of their production process? The application of the substances is as monomers for a biobased plastics material with food contact approval.
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you can ask Cargill to see if they have them.
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Endogenous is in DSM - I am interested to find out about the psychological impact of having this diagnosis, is there correlation to brain or body?  What interventions are available? Any research in relation to this diagnosis.
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Hi Maureen,
The individual (or group) psychotherapy intervention for the treatment of depression with the strongest empirical evidence is called Behavioral Activation; this is the "active ingredient" in Cognitive Behavioral Therapy. If you review the scientific literature, you will find it is as effective as medications at treating depression and more effective at preventing relapse. FYI, while there are undoubtedly cases where it makes sense to consider a purely biological source of depression (e.g., thyroid or brain disease) and to treat the underlying medical condition accordingly, there are compelling reasons to question the medical model of depression as simply reflecting a neurotransmitter imbalance (you can read more in the link below). Also, it may be worth considering that "having" everything doesn't necessarily result in fulfillment; for many (all?) people greater satisfaction is to be found in what you are "giving" through purposeful activity guided by personal values (check out the literature on Acceptance and Commitment Therapy, which combines behavioral activation, mindfulness practice, and values clarification to treat depression).
Warm regards,
Paul
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Now i am generating a DSM for Hydrodynamic Modelling so need what is RPC and what is important RPC  for Cartosat-1 data.
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Could you tell me the refinement of  RPCs with data collection  (differential GPS)
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I've recently been studying the issue of psychomotor retardation (PMR) as a symptom of depression. It seems that DSM criteria for depression include at least two other items which seem to duplicate important features of this. Both 'fatigue or loss of energy' and 'diminished ability to think or concentrate, or more indecisiveness' are both well documented elements of PMR, in which case the conflation of these items might artificially inflate the rating of depression by these criteria, and exaggerate the role that PMR symptomatology has to play.
Is this duplication and overlap of the features of depression taken into account by psychiatrists making a diagnosis of depression, or is the importance of PMR deemed high enough to warrant its common features, such as difficulty in concentrating and fatigue being - in effect - 'counted twice'?
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From a clinical perspective sometimes it would be difficult to assess separately fatigue or lack of motivation in a patient with psychomotor inhibition, because this inhibition seems to be a syndrome that includes other 'negative symptoms'. However, in the other hand it is very frequent to find depressed patients with fatigue, lack of energy, lack of interest, etc. in the absence of symptom "psychomotor inhibition"
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These following data are used for flood inundation modelling as link below.
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Hi,
You can get ALOS Global Digital Surface Model from the following website. It is of 30m.
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Hi, I’m looking for following microorganisms, maybe someone can share?:
 Elizabethkingia meningoseptica (Chryseobacterium meningosepticum)
Acetobacter aceti NBRC 14818, Gene: Abac_015_033
Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670 / NBRC 15755 / NCIMB 13165 / 161)
Acidiphilium cryptum (strain JF-5)
 Rhizoctonia solani 123EThanatephorus cucumeris (strain AG1-IB / isolate 7/3/14) (Lettuce bottom rot fungus) (Rhizoctonia solani)
Wojciech Wozny
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My dear friend: Don't try to procure from your friends. The best way is to directly approach ATCC, NBRC, NCIMB, JCM etc. You can be sure of the purity of the culture inspite of high mortality of the cell in the vials and your work will go unhindered. It is not that your friends are going to give you contaminated cultures but these organisations (listed above) are more reliable. 
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DSM - Dependency Structure Matrix
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Thank You Sir.
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I'm interested in finding a 30 meter DSM from SRTM with the hope of creating a 30 meter canopy height model. I know that it can be done because the following studies have:
and
However, I can't for the life of me find DSMs, only DEMs.  Does anyone have any suggestions for where to look online?  Or do I need to contact JPL or some other agency directly?  I'm also entertaining the idea of ASTER, but am finding those data to be equally challenging to locate.
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To summarize I followed the advice of Dean Gesch at the USGS which is also mirrored in the Kellndorfer et al. 2004 paper.  The SRTM data is the DSM.  I tried following the Kellndorfer approach and had mixed results. I took the DSM from the SRTM and then subtracted a NED DEM to try to produce a CHM (canopy height model). The results looked promising in some areas, but it was also evident that topography had a huge effect on the accuracy and needed to be dealt with in a comprehensive manner. Miliaresis 2008 http://www.mdpi.com/1424-8220/8/5/3134/htm applied a series of aspect/slope/land cover corrections and got decent results, and I attempted some of this using slope and aspect, but never quite followed through to the full extent.  I've got some concerns that using land cover to improve the CHM prediction and then using the CHM to predict land cover may be a bit circular, however.
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What software you use to prepare orthophotomaps, DTM, DSM, point cloud or 3d model with drones? I've used: Pix4D and APS.
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Hello Sebastian, yes we have to have a Remote Pilot Qualification (Ground School and Flight Test) and be approved by the CAA. This enables the correct insurance and for each operation land owner permissions and risk assessments are required. If in controlled airspace we also need to contact air traffic control. 
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There are several treatments that are most often used to manage BPD.
Borderline personality disorder (BPD) is a serious mental illness marked by unstable moods, behavior, and relationships. In 1980, the Diagnostic and Statistical Manual for Mental Disorders, Third Edition (DSM-III) listed BPD as a diagnosable illness for the first time. Most psychiatrists and other mental health professionals use the DSM to diagnose mental illnesses.
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Dear Abdelfattah,
Every 'mental' disorder (BPD)  patients need to be addressed with dire sensitivities. It is made easier if it is listed as a diagnosable DSM illness.
BPD being a 'serious' mental illness, talking with a highly dedicated and genuinely caring professionals create great trust to the patient.
To the patient, it makes it very worthwhile at one instant to know that someone sympathizes, care and most willing to 'treat' him/her. That's when the talking session, even done once would be very effective.
When this fails, there are several approaches you could apply using -
1. Dialectical Behaviour Therapy (DBT)
- treatments include individual therapy, group skills training and phone coaching
2. Cognitive Behaviour Therapy (CBT)
- treatments include recognizing and changing the patient's belief system
3. Mentalization-based Therapy (MBT)
- treatments include talking out the patient's feeling and addresing it accordingly
4. Transference-focused Therapy (TFP)
- treatment includes situational based scenarios brought upon by the therapist for the patient to apply elsewhere
5. Medications
- not useful but not detriment to include supplement like Omega 3 (not vetted) yet could relieve BPD symptoms nonetheless
6. Self-Care
- educating the patient on the importance of caring for one self  at all time
In order to minimise remissions, always refrain verbally from labeling BPD as a PSY patient to or among other healthcare team. 
This is  because such patient (knowing the gravity of this illness) will either walk away for good or die a brutal death because the doctor-patient trust and privacy have been broken totally.
Best regards - Mariam
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I'm working on a survey that provides DSM IV-TR reference because the data have been collected in 2013/2014. Could be a problem? Must we cite the classification of DSM V?
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Note that DSM-5 should be written with a "5" not "V". See here: http://www.apa.org/monitor/2013/04/dsm.aspx where they say "The idea is that there will be versions 5.1, 5.2 and 5.3 and that these sorts of mini-editions can come along more frequently than they had in the past so that the manual can be more responsive to research as things unfold"
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Can someone explain how to convert DT-DSM to CT-DSM and obtain stable Gain feedback for modulator?
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If you mean in simulink, then you only neec to replace the accumulator by ct integrator (1/s)....
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what are the differences between DSM-4 and DSM-5 regarding diagnosis of ASD?
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The Iris Center put out a great side-by-side comparison. I've attached it for you here.
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If definition of mental illness changes so that false positive / negative rates change, it may influence efficacy of drug trials, right?
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Such a 'big big question'. The response you get may also depend upon the area your respondents are in (Psychiatrists, pharmacology, psychology...). It is possible it is a wait and see...
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Recently, I 've worked with transformation of B. megaterium DSM 319. I've tried to apply protoplast transformation method and electroporation, but none gave me a good result. I am very expected of your help. If you have experience or any information about it. Thanks...
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Dear Rojas,
thanks for you recomendation. i will read it soon....
Dear Mahyad,
I have played with B. subtilis DB104 before I got B. megaterium DSM 319 project. I've found promising method for B. subtilis electroporation. You right that we must increasing the recovery time, it would give best effect to the transformation efficiency. But unfortunately I did't got  good result when applied that method to B. megaterium DSM 319. 
I treat my B.subtilis with glycine and sucrose before i did electroporation. how about you? did you give different treatment to your subtilis? maybe it will help me with this megaterium.