Science topic

DNA Repair-Deficiency Disorders - Science topic

DNA Repair-Deficiency Disorders are disorders resulting from defective DNA REPAIR processes or the associated cellular responses to DNA DAMAGE.
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I doube digested pET29C with Ndel and Kpnl-HF from NEB. However, after gel purificartion, runing the agarose gel gave me just a single band. 
I expected to see three bands represnting the isolate peice of vector around 54 pb and 5373bp which is uncut vector and 5318bp band representing the cut one. But only a single band around 5373 or 5318!!!
Can it be a true result? I mean is it possibly include uncut and cut vectors? Or my sigestion work propely and all vectors are cut?
Tnx in advance,
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Thanks a lot. 
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I found two commercial enzymes I used gave nearly same result, can't completely (100%) hydrolyze first PCR product. I have adjust the digestion condition and total volume as recommended by the manufacture but it is still not 100%
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Hi there,
2 obvious possibilities:
Your PCR product is not pure (heterozygoty might be a reason) and part of it doesn't contain the restriction site
or
time for digest is not long enough and/or enzyme is losing activity during incubation
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I am trying to describe the structure of a compound and I have trouble giving a general name to the fragment attached below. I have come up with the name cyclohexane-pyranone rings when referring to this fragment but I am not too sure if it's appropriate.
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Mr. Kacivakanadina,
You could use ACDLab (http://www.acdlabs.com/) (it is free of charge). There is able to generate the IUPAC names using 2D structures.
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Has anyone heard of this?
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Proteinase K is a fungal protease. It is difficult a contamination with mammalian chymotrypsin. However, in spite of its broad specificity, proteinase K is especially active on peptide bonds involving aliphatic and aromatic amino acids, similar to chymotrpsyn.
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Does anyone know?
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I wrote the corresponding author, but haven't received an answer yet...