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DNA Extraction - Science method

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Publications related to DNA Extraction (2,504)
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Article
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Marine sediments are complex ecosystems where structures and functions constantly change due to natural and anthropogenic influences. In this investigation, a comprehensive and comparative analysis of the bacterial communities and their functional potential of the pristine and polluted marine sediments were carried out using MiSeq. The phylum Prote...
Poster
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Objetivo Determinar la presencia de especies de Chlamydia y Mycoplasma en ranas de vidrio de zonas urbanas. Metodología Se realizó una investigación transversal descriptiva. Durante el año 2019 y 2021 se recolectaron 20 ranas de vidrio (5 H. fleischmanni y 15 E. prosoblepon) (Figura 1) en tres sitios del río Torres: La Unión de Tres Ríos (área cons...
Article
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Human tissues preserved in fixatives by and large represent an essential component in medical diagnosis and therapeutic procedures employed to study the detailed progression of diseases, or are taken during autopsies for establishing the cause of death.These archived tissue specimens constitute an invaluable resource for histological examination, m...
Preprint
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This is a protocol for extract DNA from arabidopsis thialiana.
Article
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Visando a contextualização de conteúdos com o cotidiano dos alunos como forma de proporcionar uma aprendizagem significativa, foi desenvolvida a atividade de extração de DNA de frutas com o objetivo de adaptar conteúdos e práticas científicas em linguagem informal para melhor compreensão dos discentes. Como parâmetro metodológico, utilizou-se a Seq...
Article
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Se realizó una estandarización del método de extracción de ADN para cañihua (Chenopodium pallidicaule Aellen) y la caracterización molecular mediante marcadores moleculares ISSR (Inter Simple Sequence Repeat). Se utilizaron hojas de 40 accesiones de cañihua tomadas del banco de germoplasma del Programa de Cereales y Granos Nativos de la UNALM prove...
Article
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En Andalucía se realizan diversas actuaciones para la catalogación y conservación de la biodiversidad por parte del gobierno autonómico. Con respecto a las plantas, es notable la creación de una red de Jardines Botánicos asociados a espacios naturales protegidos. Estos jardines y áreas protegidas contienen una buena representación de la flora y de...
Article
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Sub-seafloor sediment is the largest microbial habitat on Earth. The study of microbes in sub-seafloor sediment is largely limited by the technical challenge of acquiring ambient microbial DNA because of sediment heterogeneity. Changes in the extraction method, even just by one step, can affect the extraction yields for complicated sediment samples...
Article
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A perícia criminal é uma ciência que está no centro da resolução de todos os tipos de crimes, desde aqueles que envolvem vítimas fatais até aqueles relacionados ao meio ambiente. O objetivo geral do artigo foi descrever as principais técnicas moleculares padronizadas para identificação de agentes biológicos em violência sexual. Foi realizada uma re...
Article
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Chronic suppurative otitis media (CSOM) is a chronic inflammation of the middle ear and mastoid cavity, persisting for more than 2 weeks. is one of the most common organisms to cause CSOM. Carbapenems are among the most effective antibiotics used against infections resistant to carbapenems is often associated with production of metallo-ß-lactamases...
Article
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Background Next generation sequencing (NGS) is quickly coming to the forefront of diagnostic tools to provide efficient, highly informative information from patient samples. Recently, it was established that patients with pulmonary Tuberculosis (TB) have Mycobacterium tuberculosis DNA present in their stool samples, which can augment current diagno...
Article
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In recent years, many different methods that allow for the analysis of the biodiversity and structure of the community of microorganisms inhabiting the soil environment have emerged. Many of these approaches are based on molecular methods including the study of genetic biodiversity based on DNA and RNA analysis. They are superior to conventional me...
Article
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O Brasil tem como um dos pilares de sua economia o agronegócio, representando cerca de 21.4% do Produto Interno Bruto (PIB) no ano de 2019, segundo relatório do Ministério da Agricultura, Pecuária e Abastecimento. O rebanho de bovinos leiteiros confere um papel fundamental tanto economicamente, com um valor bruto de produção de 33 bilhões de reais...
Article
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Cookeina sulcipes and C. tricholoma are a cup fungi (Ascomycota) collected in Saraburi, Thailand. The fungi have been isolated, cultured and confirmed as respective species. For morphology, both Cookeina sp. are white mycelium and the growth rate on potato dextrose agar (PDA) result present C. sulcipes is faster than C. tricholoma. The molecular ch...
Poster
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Seaweed-related microorganisms secrete biologically active, useful compounds that adjust the morphogenesis of marine organisms and assist them to survive under various environmental conditions. Epiphytic microorganism plays an important role in the morphogenesis and the growth of seaweed. We collected green marine seaweed (Enteromorpha prolifera) i...
Article
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Abstract: Conventional and commercially available DNA extraction methods have several limitations regarding, for instance, contamination, and complex and slow precipitation and recovery processes. Herein, we report the synthesis of oxygen and phosphorus-doped Graphitic carbon nitride structures (g-POCN), via a novel Zinc-catalyzed one-pot solvother...
Article
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Microbiome studies have contributed to many fields, such as healthcare and medicine; however, these studies are relatively limited in forensics. Microbiome analyses can provide information, such as geolocation and ancestry information, when short tandem repeat (STR) profiling fails. In this study, methods for DNA extraction and sampling from the sk...
Article
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Isolating DNA from bacterial cells concentrated directly from water samples allows the analysis of the DNA with a range of molecular biology techniques. The aim was to develop a cost-effective method to concentrate bacterial cells directly from water for DNA extraction and PCR amplification. A modified in-house guanidinium thiocyanate DNA extractio...
Article
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In Iraq, Awassi is the most widespread sheep breed on non-European origin. The breed adapt to a wide range of environmental conditions. Sheep farming, mainly sheep of Awassi breed, represents an essential economic activity of Iraq; however, so far, only very few genetic data exist on this breed. The present study was performed in Biology Department...
Article
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Visceral leishmaniasis remains a serious public health issue, and Brazil was among the seven countries with the highest prevalence of this disease worldwide. The measures to control this disease are not easily developed, and the improvement of its diagnosis, surveillance, and control is still needed. This study aimed to carry out the polymerase cha...
Article
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Background: The prevailing methods for the genomic DNA extraction of Sclerotium rolfsii from its mycelium mat is often time consuming and yields poor quality and quantity of genomic DNA owing to presence of the outrageous magnitude of mucilage and polysaccharides. Methods: A new fast track method for DNA isolation from the resting structure (sclero...
Article
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Stool samples typically contain PCR inhibitors; however, helminths are difficult to lyse and can cause false-negative PCR results. We assessed the effective methods for extracting DNA from different kinds of intestinal parasites. We compared the most common DNA extraction methods from stool samples, including the phenol-chloroform technique with or...
Presentation
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Obter DNA com qualidade e quantidade adequadas para as análises moleculares é, em muitos casos, um desafio para espécies ricas em metabólitos secundários, pois estes interferem na extração de um DNA puro e íntegro, além da necessidade do uso de reagentes tóxicos e muitas etapas purificação desses compostos. Diante disso, o objetivo deste trabalho f...
Preprint
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Below you will find a simple and fast two-step DNA extraction protocol that works well with many different groups of fungi. This protocol can be utilized in preparation for DNA barcoding of fungi utilizing Oxford Nanopore MinION. Time required: Varies depending on the use case. My samples generally have the metadata and images stored on iNaturalist...
Article
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Los hongos son los principales degradadores de la madera en los ecosistemas bos- cosos, contribuyendo significativamente al ciclo global del carbono. Los enfoques metagenómicos basados en un amplicón específico (metabarcoding) constituyen una herramienta poderosa para su prospección y estudio. El objetivo principal de este estudio fue caracterizar...
Article
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The PrepFiler™ BTA Forensic DNA Extraction Kit (BTA) is designed to purify DNA from challenging forensic samples including tissue, bone, tooth and adhesive-containing substrates. Earlier versions of the manual state that lysates are stable at ambient temperature for 24 h when 300 µL of PrepFiler™ lysis buffer is added to the lysate. The effect of c...
Article
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DNA analyses and DNA-based diagnoses are going forwardly in the commercial deals with shrimp (Penaeus monodon) as well as in the basic research. In this regard extraction of DNA is obvious. However, numerous protocols are available DNA extraction from animal tissue. Therefore, which technique is suitable one for shrimp tissue is indispensable to ju...
Article
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Introdução: Os vegetais minimamente processados são cada vez mais reconhecidos como importantes veículos para a transmissão de patógenos humanos. A importância clínica de S. maltophilia, P. aeruginosa e Salmonela spp. tem sido reconhecida por serem relevantes microrganismos patógenos oportunistas. Sabe-se que a alimentação é a principal forma para...
Article
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Objective: The present study was conducted to examine the association between the IL-10-1082A>G (rs 1800896) polymorphism and risk of Chronic Lymphocytic Leukemia and to assess the correlation between this polymorphism and clinicopathological characters. Methods: A case-control study was conducted in Khartoum state, Sudan, during the period from...
Article
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This study assessed the performance of five different DNA extraction methods for the recovery of DNA from bone: ChargeSwitch® gDNA Plant Kit, DNA IQTM System Kit, DNeasy® Blood & Tissue Kit, PrepFiler® BTA Forensic DNA Extraction Kit and phenol-chloroform-isoamyl alcohol. DNA was extracted from pig rib and femur bones that was fresh, had undergone...
Article
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Background: several studies have been performed to investigate the association of TNF-α-308G>ASNP and CLL susceptibility However, the results are inconsistent. This study aimed to investigate the association between TNF-α-308G>ASNP of the TNF-α gene and CLL risk in the Sudanese population and correlated genotypes with clinicopathological features....
Article
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The regulatory HERC2 SNP, rs12913832, is strongly associated with blue and brown eye colour. However, eye colour in heterozygous rs12913832 individuals is observed to vary greatly. Missense mutations in OCA2, such as rs1800407 and rs74653330, are associated with lighter eye colour in some but not all heterozygous rs12913832 individuals. Determining...
Article
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The PortaLyzer is a portable homemade device that allows researchers to perform bead-beating steps commonly found in environmental DNA (eDNA) extraction protocols in the field without access to power. This allows researchers to preserve in situ organism abundance by beginning eDNA extraction quickly. The PortaLyzer is composed of a variable speed,...
Article
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A mandioca (Manihot esculenta) é amplamente distribuída no Brasil, nas roças encontram-se plantas com características diferentes que são mantidas e propagadas entre os agricultores. Levando em consideração a diversidade de etnovariedades encontrada, esse trabalho teve como objetivo realizar a caracterização molecular de etnovariedades de mandioca c...
Article
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Direct DNA analysis is the most widely used approach for microorganism characterization in natural and built environments; therefore, reliable and effective methods of nucleic acid extraction for samples from particular types of environments are needed. In this study, we compared three commercial kits for metagenomic DNA extraction from three types...
Article
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Poster session 3, September 23, 2022, 12:30 PM - 1:30 PM Objectives To identify cryptic species of the genus Aspergillus using Beta-tubulin gene by sequence typing. Methods Aspergillus grown from various clinical samples (Ear swab, Bronchial wash, Endotracheal Aspiration, Paranasal sinus, BAL, Sputum) was subcultured on Sabouraud's Dextrose Agar/...
Article
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DNA extraction is a crucial stage in plant molecular analysis activities that determine the success of further downstream analysis stages. Molecular analysis activities, including Polymerase Chain Reaction (PCR), restriction digestion, DNA hybridisation, genomic library construction, and DNA sequencing, mostly require DNA with high quality and enou...
Poster
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Friction skin ridge patterns are considered one of the most relevant physical evidence, with donor identification potential, that can be recovered from crime scenes. Indeed, for a century, fingerprints have been used to exclude or individualize a suspect during an investigation. Currently, they may not only provide a unique ridge pattern through mi...
Article
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Background: Bronchopulmonary dysplasia (BPD) is a chronic lung disease affecting primarily preterm and very low birth weight (VLBW) infants. Despite the advances in perinatal care, BPD remains a major clinical and costly complication in premature infants. The pathogenesis of BPD is complex and multifactorial. Prematurity, mechanical ventilation, o...
Article
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Recent studies have documented an increase in the incidence of antifungal resistance in newly emerging species closely related to C. albicans, and the coexistence of genotypic variants. Hence, an application of PCR-based molecular typing is crucial in identifying these fungi. Our study used molecular methods to characterize the latest genotypic sub...
Article
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Granular ark (Tegillarca granosa), broughton’s ribbed ark (Anadara broughtonii), and half-crenate ark (Anadara kagoshimensis) are important fishery resources throughout Asia; granular ark exhibiting a higher economic value due to its rarity. However, due to the similar morphological characteristics of the three species, the less valuable species co...
Article
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Ferramentas de identificação baseadas em DNA são de suma importância em estudos genéticos de plantas, onde o isolamento e a purificação são passos cruciais. O objetivo do presente estudo foi testar a eficiência de métodos de conservação e de maceração de materiais biológicos na extração de DNA genômico de folha e estipe de x Butyagrus nabonnandii (...
Article
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La palma Brahea berlandieri Bartlett (Arecaceae) se encuentra enlistada en la nom-059-semarnat-2010, bajo la categoría de riesgo: Sujeta a protección especial (Pr); es una planta endémica que sólo se ubica en los bosques de encinos de la Sierra Madre Oriental, principalmente del noreste de México. Las palmas de esta especie son afectadas por factor...
Article
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Deoxyribonucleic acid (DNA) extraction, which is the mainstay of molecular amplification for food pathogen detection procedure, is dependent on laboratory infrastructure, cumbersome methods, and time consumption. To tackle these dependencies, we developed an enhanced mussel-inspired cellulose card for the convenient, eco-friendly, and rapid DNA ext...
Article
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Purpose: The main aim of the study was to evaluate the potential roles of KRAS/NRAS proto-oncogenes, IL-4 VNTR variants and HPV prevalence in colorectal cancer metastasis. As the second aim, the interactions of the analyzed genes and viral sequences with both clinicopathological variables and each other were targeted. Methods: DNA was extracted...
Article
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The aim of this study was to evaluate the performance of two of the most commonly used commercial kits for soil DNA extraction regarding the values of the taxonomic diversity of prokaryotes and community composition of saffron (Crocus sativus) cultivated fields. The impact of the QIAGEN-DNeasy PowerSoil Kit (MO) and Macherey-Nagel™ NucleoSpin™ Soil...
Article
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For-profit donor human milk organizations have DNA-based proprietary methodology for testing incoming milk for adulteration with other species’ milk. However, there is currently no standardized methodology for extracting DNA from human milk. Microbiome research has shown that DNA purity and quantity can vary depending on the extraction methodology...
Article
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Introducción: La prevalencia de los diferentes genotipos de virus del papiloma humano (VPH) varía dependiendo de la severidad de la lesión y región geográfica. Objetivo: Identificar infecciones múltiples de VPH en lesiones cervicales de bajo y alto grado en un grupo de mujeres del Bajío mexicano referidas con citología no concluyente. Métodos: Es...
Article
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Beef is one of the animal protein sources needed by the body to meet nutritional requirements. Consequently, fresh beef (whole or milled) is one of the highest-demanded comestibles on the market. Some meat kiosks in Yogyakarta traditional markets are also in service of milling whole meat. However, reports are stating that some kiosks are neglecting...
Article
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(1) Background: Extracellular vesicles (EVs) have emerged as crucial players in the communication between cells in both physiological and pathological scenarios. The functions of EVs are strongly determined by their molecular content, which includes all bioactive molecules, such as proteins, lipids, RNA, and, as more recently described, double-stra...
Conference Paper
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Acidovorax citrulli é o agente causal da mancha aquosa, doença que afeta principalmente o meloeiro e a melancieira e é de grande importância para a região Nordeste do Brasil. Duas linhagens distintas em agressividade e preferência por hospedeiros são relatadas nessa bactéria, em que o grupo I é formado principalmente por isolados de meloeiro e outr...
Article
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Iron oxide nanoparticles coated with polyethylene glycol were synthesized by coprecipitation for use in the magnetic separation of DNA (deoxyribonucleic acid). The blocking temperature of nanoparticles was studied by the methods of Mössbauer spectroscopy, ferromagnetic resonance, and using magnetometric measurements. The blocking temperature calcul...
Article
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El objetivo del presente estudio fue detectar ADN de Toxoplasma gondii en muestras de músculo esquelético y de riñón recolectados de pequeños roedores silvestres, capturados en 10 localidades de la península de Yucatán, México (estados de Campeche, Quintana Roo y Yucatán) en 2018-2019. Se capturaron 127 roedores pertenecientes a siete especies. A t...
Article
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Los nematodos constituyen un importante problema de salud pública, por ello, la identificación del Toxocara canis de aislamientos en caninos del cantón Salcedo, provincia Cotopaxi, Ecuador, mediante la secuenciación de la región del segundo espaciador transcrito interno (ITS-2) del ADN ribosómico (ADNr), constituye una herramienta eficaz abordada e...
Article
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Background: DNA extraction is the process by which the DNA is extracted and purified from the living or dead cells or tissues. There are many different kits commercially available for DNA extraction, with varying protocols. This study aimed at determining the optimal conditions for the extraction of high yield DNA from banana fruits. Methodology: D...
Article
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Tick-borne infections are a serious threat to humans, livestock, and companion animals in many parts of the world, often leading to high morbidity and mortality rates, along with decreased production values and/or costly treatments. The prevalence of the microbes responsible for these infections is typically assessed by the molecular identification...
Article
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In terms of species identification, the ultimate aim of extracting DNA is the subsequent amplification of the selected marker; therefore, the quality and quantity of the extracted DNA must be sufficient for PCR-based methods. The purpose of this study is to compare five DNA extraction methods according to the parameters of quantity, quality and sim...
Article
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DNA based sequencing technology has revolutionized the field of microbial ecology and environmental studies. However, biases can be introduced at all experimental steps and, thus, affect the interpretation of microbial community. So far, previous studies on the biases introduced from the key steps of DNA extraction and primer sets mainly focused on...
Article
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Background: Cryptosporidium is an etiologic agent of gastro-intestinal disorders and severe, profuse watery diarrhoea in immunocompromised patients, such as people living with HIV/AIDS. Aim: This study was carried out to determine the genotypic and phylogenetic characteristics of Cryptosporidium parvum among HIV patients who usually received antire...
Article
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Metagenomic next-generation sequencing (mNGS) is a novel useful strategy that is increasingly used for pathogens detection in clinic. Some emerging mNGS technologies with long-read ability are useful to decrease sequencing time and increase diagnosed accuracy, which is of great significance in rapid pathogen diagnosis. Reliable DNA extraction is co...
Article
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The use of combined antiretroviral therapy (cART) has turned HIV infection to a manageable condition, significantly reducing HIV-related morbidity and mortality globally. Risk of transmission has been substantially reduced in Africa where 67% of global infection is domiciled. However, long-term impact of cART on life quality of HIV patients elicits...
Presentation
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El género Bartonella comprende bacterias gramnegativas transmitidas por vectores que infectan los eritrocitos y las células endoteliales de los mamíferos hospedadores. Los roedores son reservorios naturales de muchas especies de Bartonella, y las pulgas (Siphonaptera) tienen un rol clave, ya que demuestran una gran eficacia enel ciclo de transmisió...
Article
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‘Candidatus Liberibacter solanacearum’ (CaLsol) is an uncultured bacterium, transmitted by psyllids and associated with several diseases in Solanaceae and Apiaceae crops. CaLsol detection in psyllids often requires insect destruction, preventing a subsequent morphological identification. In this work, we have assessed the influence on the detection...
Article
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Long-read DNA sequencing technologies require high molecular weight (HMW) DNA of adequate purity and integrity, which can be difficult to isolate from plant material. Plant leaves usually contain high levels of carbohydrates and secondary metabolites that can impact DNA purity, affecting downstream applications. Several protocols and kits are avail...
Article
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Objetivo. Informe sobre especies de garrapatas que parasitan a los humanos en un área del bioma semiárido de Caatinga, noreste de Brasil, con nota de infección por rickettsias. Materiales y métodos. Todas las garrapatas se identificaron morfológicamente con estereomicroscopio. Algunas de las garrapatas recolectadas se analizaron molecularmente para...
Article
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Recovery and amplification of nucleic acids from archived formalin-fixed tissue samples is the most developing field in retrospective genetic studies. We compared different deparaffinization methods and DNA isolation techniques, and intergroup comparisons were performed to evaluate the effectiveness of different storing methods for archival OSCC sa...
Article
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Oesophageal cancer is the sixth most common cancer among males and the ninth among the females worldwide. The purpose of this study is to detect association of Human papilloma virus subtype 16 and esophageal squamous cell carcinoma in Khartoum state Hospitals by polymerase chain reaction method. A retrospective descriptive study of archival formali...
Article
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Introducción. El consorcio europeo BIOMED-2 se creó para determinar si una población linfoide de difícil clasificación patológica es clonal. En Colombia, la implementación de estas pruebas comenzó en el 2015 en el Instituto Nacional de Cancerología E.S.E. (Bogotá). Objetivos. Determinar el comportamiento de las pruebas de reordenamiento clonal o cl...