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Publications related to Cytological Techniques (3,901)
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In many diseases such as interstitial lung diseases (ILDs), patient diagnosis relies on diagnostic analysis of bronchoalveolar lavage fluid (BALF) and biopsies. In BALF the differentiation of neutrophils, eosinophils, lymphocytes, and macrophages can contribute to diagnose the underlying ILD entity. To analyze the BALF standard cytological techniqu...
Article
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Programmed death ligand-1 (PD-L1) immunostaining, which aids clinicians in decision-making on immunotherapy for non-small cell lung cancer (NSCLC) patients, is sometimes performed on cytological specimens. In this study, differences in cytology fixation and cell block (CB) processing between pathology laboratories were assessed, and the influence o...
Article
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Adenocarcinoma in situ (AIS) is considered a precursor of adenocarcinoma. Cervical adenocarcinoma has been associated with human papillomavirus (HPV), while other subtypes of AIS and endocervical adenocarcinoma have no precursor lesions and are not associated with HPV. Cervical cytology and HPV genotyping are important in the detection of these dif...
Article
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Sheath blight, caused by Rhizoctonia solani , is a big threat to the global rice production. To characterize the early development of R. solani on rice leaf and leaf sheath, two genotypes, GD66 (a resistant genotype) and Lemont (a susceptible genotype), were observed using four cytological techniques: the whole-mount eosin B-staining confocal laser...
Article
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Background: Malignant pleural effusion missed routinely because of less diagnostic yield of conventional fluid cytology. Materials and Methods: Prospective multicentric study conducted during January 2014 to June 2016 in Venkatesh chest hospital, and Pulmonary Medicine, MIMSR medical college Latur, to find diagnostic yield of conventional pleural f...
Article
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Background: The management of patients with "Atypical Squamous Cells" (ASC) in conventional papanicolaou smears (CPS) is based on the risk of high-grade squamous intraepithelial lesion (HSIL). The efficacy of liquid-based cytology (LBC) to detect this premalignant lesion is variable, with little evidence of its performance in Colombian patients....
Article
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Somatic embryogenesis (SE) is a complex process that begins with regaining totipotency in some somatic cells, proceeds through embryo development and maturation, and ends with the formation of a whole plant. Since the first publications on SE in 1958, this regeneration process has been applied to the in vitro propagation of many plant species and h...
Article
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Received: June 11 th 2022 Oral mucosal lesions are caused by irritation from malocclusion or orthodontic appliances. Wearers of orthodontic appliances some times are suffering from mucosal lesions, buccal mucosa of orthodontics patient have many pathological change such as binucleation and micronuclei and sveral types of inflammatory cell .Aims of...
Article
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Primordial germ cells develop into oocytes and sperm cells. These cells are useful resources in reproductive biology and regenerative medicine. The mesenchymal stem cells (MSCs) have been examined for in vitro production of primordial germ cell-like cells. This study aimed to summarize the existing protocols for MSCs differentiation into primordial...
Preprint
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Purpose: Oral squamous cell carcinoma (OSCC) sum 90 to 95 percent of oral cancers and the incidence described men aged 40 to 60 years old. The greater risk factor is the use of tobacco and alcohol, and they are described in independent or combined action. The gold standard method for OSCC diagnosis remain biopsy and histopathological examination. V...
Article
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Biomarker testing is crucial for treatment selection in advanced non-small cell lung cancer (NSCLC). However, the quantity of available tissue often presents a key constraint for patients with advanced disease, where minimally invasive tissue biopsy typically returns small samples. In Part 1 of this two-part series, we summarise evidence-based reco...
Article
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Background: Bronchogenic cancer is the most common cause of cancer-related mortality in both sexes all over the world, especially across urban population. However, early diagnosis and timely intervention may minimize the mortality rate to a certain extent and increase the 5-year survival rate to 70–80%. Among the diagnostic modalities available, br...
Article
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Introduction: Body fluids are processed by routine cytology techniques like preparing a slide and staining it with PAP stain. By this method it is difficult to give definitive diagnosis particularly to the cases where some abnormal cells seen suspicious of malignancy. In our study we utilized cell lock technique for reporting body fluid cytology. O...
Article
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Cervical cancer is the third most common cancer in women worldwide. Conventional cytological examination as a screening method with Papanicolaou has been established to reduce the incidence of dysplasia and cervical cancer for years. In addition to the conventional screening, the introduction of immunocytochemical examinations, including CINtecPlus...
Article
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Introducción. La detección del virus del papiloma humano mediante la combinación de la prueba de HPV y otras técnicas como la citología, ha demostrado su eficacia en el diagnóstico y tratamiento oportuno de lesiones asociadas con el cáncer de cuello uterino. Objetivo. Estimar el impacto presupuestal de la estrategia de detección temprana del HPV me...
Chapter
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The basidiomycete Coprinus cinereus has some special features which particularly suit it to experimental studies. In addition to ease of culture, which makes conventional approaches with various cytological techniques (including continuous video observation) practicable, we show in this chapter how a number of transplantation and bioassay experimen...
Article
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Classifying and analyzing human cells is a lengthy procedure, often involving a trained professional. In an attempt to expedite this process, an active area of research involves automating cell classification through use of deep learning-based techniques. In practice, a large amount of data is required to accurately train these deep learning models...
Article
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Patients with new-onset malignant spinal lesions often have an urgent need for local spine intervention and systemic therapy. For optimal management, it is crucial to diagnose the underlying disease as quickly and reliably as possible. The aim of our current study was to determine the feasibility, sensitivity, specificity, and diagnostic certainty...
Article
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Most oral squamous cell carcinomas (OSCCs) arise from a premalignant lesion, oral epithelial dysplasia; however, useful markers for the early detection of OSCC are lacking. The present study aimed to establish a novel experimental model to observe changes in the sequential expression patterns of mRNAs and proteins in a rat model of tongue cancer us...
Article
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Sweat glands play an important role in thermoregulation via sweating, and protect human vitals. The reduction in sweating may increase the incidence of hyperthermia. Myoepithelial cells in sweat glands exhibit stemness characteristics and play a major role in sweat gland homeostasis and sweating processes. Previously, we successfully passaged prima...
Article
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Colorectal carcinoma (CRC) is one of the main public health problems. The mortality of CRC is about 8%. Early detection of CRC is very important to prevent death because this cancer could be cured through surgery if the diagnosis can be made as early as possible. Therefore screening strategy for early detection of CRC is critical in reducing mortal...
Article
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Background: Malignant pleural effusion missed routinely because of less diagnostic yield of conventional fluid cytology like fluid cytology. Fluid cell block is underutilsed techniques routinely and less utilized in diagnostic panel due to lack of expertise in filed of cytopathology. Materials and methods: Prospective multicentric study conducted d...
Chapter
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This chapter aims to provide a complete knowledge over the primary intraocular lymphoma (PIOL) and a correct clinical approach towards this rare condition, to avoid delays in diagnosis, which is considered the most important prognostic factor. A PIOL arises with no specific symptoms and could mimic both inflammatory and non-inflammatory ocular cond...
Article
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Frozen section (FS) is a prominent point of intersection between surgeons and pathologists. It is regarded as the most denitive but not the sole form of intraoperative consultation. It plays an important role in tissue triage, diagnosis, and intraoperative management. Intraoperative consultation guides immediate surgical management to establish na...
Article
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Consumption of very hot beverages and foods increases the incidence of oral and esophageal cancer but the mechanisms are not known and the critical temperature is not well defined. We realized a study with exfoliated cells from the oral cavity of individuals (n = 73) that live in an area in Iran which has the highest incidence of EC worldwide. Cons...
Article
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Purpose: B-cell receptor (BCR) signaling is critical for the pathogenesis of chronic lymphocytic leukaemia (CLL), promoting both malignant cell survival and disease progression. Although vital, understanding of the wider signaling network associated with malignant BCR stimulation is poor. This is relevant with respect to potential changes in respo...
Article
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Background Lung cancer is still the main cause of cancer deaths. The high lung cancer mortality rate is caused by a diagnosis factor or therapy selection. The cell block cytology technique using fine needle aspiration (FNA) samples can provide immunocytochemical material that plays an important role in the differential diagnosis of lung cancer subt...
Article
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Cytology samples are suitable for the study of genotypic and phenotypic changes observed in different tumors. Being a minimally invasive technique, cytology sampling has been used as an acceptable alternative to track the alterations associated with tumor progression. Although the detection of gene mutations is well-established on cytology, in the...
Article
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Progressive multifocal leukoencephalopathy (PML) is a central nervous system demyelinating disease primarily affecting immunocompromised individuals, including those with human immunodeficiency virus (HIV) infection, rheumatological diseases, or hematological malignancies.1,2 The disease is caused by JC polyomavirus (JCV), and oligodendroglial intr...
Article
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Oral malignancy is very common in India because of the excessive use of tobacco chewing and smoking. Procedure of Cytology is inexpensive and unchallenging that can be carried out effortlessly at outdoor patient department to diagnose malignancy at early stage. The present study was carried out to detect cancer pre-invasive stage by use of exfoliat...
Article
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BACKGROUND Differentiation of benign and malignant biliary strictures plays a pivotal role in managing biliary strictures. Brush cytology via endoscopic retrograde cholangiopancreatography (ERCP) and endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) are two diagnostic methods. In the present study, we aimed to compare the accuracy of th...
Article
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Sample barcoding is essential in mass cytometry analysis, since it can eliminate potential procedural variations, enhance throughput, and allow simultaneous sample processing and acquisition. Sample pooling after prior surface staining termed live-cell barcoding is more desirable than intracellular barcoding, where samples are pooled after fixation...
Article
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Kersting’s groundnut (Macrotyloma geocarpa) is an orphan crop of significant food and household income in sub-Saharan Africa. The objective of this study was to evaluate the chromosomal variability among the accessions of the species through analysis of the karyotypes. Six accessions of Kersting’s groundnut were subjected to conventional cytologica...
Article
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Kersting’s groundnut (Macrotyloma geocarpa) is an orphan crop of significant food and household income in sub-Saharan Africa. The objective of this study was to evaluate the chromosomal variability among the accessions of the species through analysis of the karyotypes. Six accessions of Kersting’s groundnut were subjected to conventional cytologica...
Article
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The current study was performed on eight years old peach ( Prunus persica L. Batsch) trees cv. Florida prince to study the influence of spraying of commercial nano fertilizer on vegetative growth, pollen grain viability, yield, and fruit quality of the "Florida prince" peach cultivar. Furthermore, extracts from the nanofertilizer treated leaves wer...
Article
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Oral and oropharyngeal cancer, grouped together, are the sixth most common cancer in the world. Oral squamous cell carcinoma (OSCC) accounts for more than 95% of all oral cancers. OSCCs diagnosed at a late stage often results in a diminished quality of life and impaired function. OSCC could be preceded by oral potentially malignant disorders (OPM...
Article
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While rapid on-site evaluation (ROSE) is considered to be an additional tool to optimize the yield of tissue acquisition during EUS-guided FNA of the gastrointestinal tract (1)(2) it is not readily available at all times while performing these procedures. We reviewed twenty-seven EUS-guided FNA procedures done at our institution in Tripoli central...
Article
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Background: Differentiating between malignant and benign salivary gland tumors with fine-needle aspiration cytology (FNAC) can be challenging. This study was aimed at testing the validity of the Milan System for Reporting Salivary Gland Cytopathology (MSRSGC) and at assessing possible differences in the sensitivity and specificity of parotid gland...
Chapter
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RNA in situ hybridization (RISH) is a cytological technique to visualize the subcellular distribution and abundance of specific RNA molecules in cultured-cells or tissues. The ability to ‘see’ the localization of specific RNA species and quantify the same in different cell types has contributed immensely to our understanding of gene expression and...
Article
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We evaluated the capacity of the XN-350 instrument to analyze 3 different types of body fluid samples under "body fluid mode."The performance of XN-350 was evaluated in terms of precision, carryover, limit of blank, limit of detection, limit of quantification, and linearity. Cell enumeration and differential data produced by the XN-350 were compare...
Article
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The structural integrity of the host red blood cell (RBC) is crucial for propagation of Plasmodium spp . during the disease-causing blood stage of malaria infection. To assess the stability of Plasmodium vivax -infected reticulocytes, we developed a flow cytometry-based assay to measure osmotic stability within characteristically heterogeneous reti...
Article
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Background: Bacterial vaginosis (BV) is a common infection in women during their reproductive age. However, the burden of BV among HIV-positive women in Kenya is not clear. This study aimed to determine the prevalence of BV in HIV-positive women visiting the comprehensive care center in Machakos county hospital, Kenya. Study Design: Cross-sectiona...
Article
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Cell-block preparations have become an essential part of integrated cytology diagnosis. They are essentially microbiopsies that are formalin fixed and embedded in paraffin. This has become more prevalent with greater sample procurement due to the advent of newer biopsy techniques and needles. Cell-blocks allow retrieval of small tissue fragments fr...
Article
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Purpose of review: Endothelial cell (EC) front-rear (axial) polarization in response to chemokines and shear stress is fundamental for angiogenesis. This review provides an overview of the in vitro and in vivo methods that are currently available to quantify EC axial polarity. Recent findings: Innovative methodologies and new animal models have...
Article
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Background: Clinicians face several dilemmas regarding tracheal washes (TWs) for the diagnosis of respiratory disease, including method and prediction of bacterial growth from cytology results. Objective: To compare cytology and culture of endotracheal and transtracheal washes and identify factors associated with discordancy and bacterial growth...
Article
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MicroRNAs (miRNAs) are cargo carried by extracellular vesicles (EVs) and are associated with cell–cell interactions. The response to the cellular environment, such as disease states, genetic/metabolic changes, or differences in cell type, highly regulates cargo sorting to EVs. However, morphological features during EV formation and secretion involv...
Article
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Aims The diagnosis of metastatic cutaneous melanoma (CM) on lymph node fine needle aspiration samples may be challenging and usually requires confirmation by immunocytochemistry. However, the cytological material could be too scant to order a broad panel of markers. In this case, the pathologist is forced to choose the most advantageous antibodies....
Article
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Cell shape is crucial for the function and development of organisms. Yet, versatile frameworks for cell shape quantification, comparison, and classification remain underdeveloped. Here, we introduce a visibility graph representation of shapes that facilitates network-driven characterization and analyses across shapes encountered in different domain...
Article
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Prostate cancer is a life-threatening and highly heterogeneous malignancy. In the past decade, circulating tumor cells (CTCs) have been suggested to play a critical role in the occurrence and progression of prostate cancer. In particular, as the “seed” of the cancer metastasis cascade, CTCs determine numerous biological behaviors, such as tumor inv...
Article
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Endometritis is associated with fertility problems in many species, with endometrial biopsy being the main diagnostic tool. In feline queens, the reduced size of the uterus may make it difficult to obtain representative diagnostic samples. Endometrial cytology may represent a valuable diagnostic tool for evaluating the health status of the endometr...
Article
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Background and aim: Colorectal cancer (CRC) accounts for over 8% of all deaths each year, with 1.2 million new cases diagnosed annually worldwide. It represents the seventh most common cancer in Egypt. Early detection of peritoneal metastasis is a major challenge in such cases. It helps with the decision of the immediate application of intraperito...
Article
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Objectives: Atypical squamous cells of undetermined significance (ASC-US) represent a diagnostic challenge during cervical cytology. This study aimed to review and identify high-risk human papillomavirus (HR-HPV) genotypes among previously diagnosed ASC-US cases in Kuwait. Methods: This retrospective study analysed 180 cases diagnosed as ASC-US...
Article
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Context: Eyelids by virtue of its unique histomorphology give rise to multitudes of different pathological conditions. Appropriate preoperative cytological diagnoses of these cases are necessary to determine their optimal therapy. Aims: The aims of this study were to evaluate the utility of various cytological techniques in diagnosing the defini...
Article
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The Brassica coenospeceis have treasure troves of genes that could be beneficial if introgressed into cultivated Brassicas to combat the current conditions of climate change. Introducing genetic variability through plant speciation with polyploidization is well documented, where ploidy augmentation of inter-generic allohexaploids using somatic hybr...
Article
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Purpose: Mouse embryonic fibroblasts (MEFs) accessibility coupled with their simple generation make them as a typical embryonic cell model and feeder layer for in vitro expansion of pluripotent stem cells (PSCs). In this study, a mechanical isolation technique was adopted to isolate MEFs and the efficiency of this technique was compared with enzyma...
Article
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Objective: To obtain data about the evaluation of thyroid nodules (TNs) in the northeastern of the State of São Paulo, compared by health care type, and measure the performance of cytology as a screening test for thyroid cancer (TC). Subjects and methods: We collected data of 597 patients treated in the Brazilian public health care system (SUS), s...
Article
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Morphometry and histology are essential approaches for investigation and diagnosis of musculo-skeletal disorders. Despite the advent of revolutionary methods of image analysis and high resolution three-dimensional imaging technology, basic conventional light microscopy still provides an incisive overview of the structure and tissue dynamics of the...
Article
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Objectives: To evaluate the accuracy and e cacy of ne-needle aspiration cytology (FNAC) in diagnosing thyroid nodules, correlating it with the histopathological findings. Methods: A retrospective evaluation of 314 patients was undertaken at a tertiary referral center of King Abdullah Medical City (KAMC), Makkah, Kingdom of Saudi Arabia, between...
Article
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Intra operative cytology is an important diagnostic modality especially central nervous system tumors. Squash smears most widely used cytological technique is rapid, accurate and relatively uses little tissue. It also helps the surgeon to plan the extent of surgery. To assess the accuracy of squash smear by comparing with the histopathological diag...
Article
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Molybdenum (Mo) is a component of the Mo cofactor (Moco) of nitrate reductase (NR) and is therefore essential for nitrate metabolism. However, little is known about Mo deficiency phenotypes or about how physiological and molecular mechanisms of Mo uptake and transport influence nitrate uptake and utilization in strawberry. Here, we used physiologic...
Article
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The last two decades saw the establishment of three-dimensional (3D) cell cultures as an acknowledged tool to investigate cell behaviour in a tissue-like environment. Cells growing in spheroids differentiate and develop different characteristics in comparison to their two-dimensionally grown counterparts and are hence seen to exhibit a more in vivo...
Article
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Context: Intraoperative pathological assessment provides valuable information in a patient's diagnosis and management. Touch imprint/crush cytology is simple, rapid, and cost-effective. Also, imprint smears give excellent cytomorphology. Aims: To assess the utility and feasibility of intraoperative cytology technique as a rapid and reliable meth...