Questions related to Cysts
What is the recorded unit area production of Artemia cyst in different culture conditions? any review articles. kindly suggest please.?
PCN cyst is the main survival component of this pest, it comes out of cyst on chemical signal from roots of potato or some other crops and attack roots, forms cyst again. any of the nematicide which can work on cyst in soil or either a chemical which can dissolve the cyst in soil will be effective measure all over world PCN control by crop rotation, trap crops and nematicides only help in controlling at one level but its eradication is not possible. So the area where once infestation is reported can never be used as area for potato breeder seed production as per Breeder seed production protocols. So my query stands at a point of application of chemical for either dissolving the cyst or signal it to hatch will help. Any such solution may plz be forwarded.
Splenic cysts found incidentally on CT scan, do they need to be followed, treated or worked-ip?
I am working on 3D culture of MDCK cysts. Whenever i try to fix the cells with PFA, the matrix depolymerizes. We thought it was something to do with the matrigel, so we tested different lots as well as the Cultrex matrix, but it was the same case for all. We are pretty confused how all these papers report fixing cysts with 4% PFA, in our hands the matrix dissolves even at 0.2% PFA concentration!
We are able to culture cysts well, but have no idea how to fix and stain them further. Methanol fixation won't work since it dehydrates the cysts and cause them to collapse. Formalin fixation didn't help either. Please advise!
In case of Toxoplasma gondii infection tissue cysts produced as a defense mechanism against the host immune response .
A women of 40 years refered with expansion in right maxilla and in palpation fluctuant in some part and in some area is bony hard .
Endometriosis is defined and diagnosed surgically by the appearance of endometrial-like stroma and glands in the sites exterior to the uterus (Denny et al, 2007). It would be interesting to find out and learn the diagnosis, treatment, prevalence of endometriosis in Indian women. Also, are they being referred to a women's health physiotherapist?
Simply curious. Also, it will help me understand the nature of cysts for a research on the effectivity of Eleusine indica in the treatment of ovarian cysts.
Does anyone know about research to this topic or has tried this before?
I wondered if there was a way to make the cysts more visible among other organic compunds in the soil samples for the diagnistics in the EPPO / plant health monitorings. We have a lot of other floating organic remains in our soil extracts. Thus I am thinking about (several) new ways to cope with this.
Thank you very much in advance, for any extra information - positive or negative - that might result! :)
PS: We use a MEKU (a decanting technique) to extract the cysts from soil and then examine the sample on paper. Unfortunately we have very much plant material in those extracts as well. That's why I had the idea "if only these cysts would fluoresce under e.g. UV, while the other compounds didn't, then I would find the cysts much faster"
Probably a silly question but I am a complete novice in molecular biology. I have decided to attempt a phylogenetic analysis. I have managed to create a phylogenetic tree but want to confirm whether it is detrimental or not to align both types of sequences.
For some context I have collected 87 sequences from a frog family. Some of these species are fairly unknown so the choices for DNA sequences are limited.
I have got RAG1, Cyst and 16S rRNA sequences so far and my tree looks okay (as far as I'm aware).
Dinoflagellate cysts are supposed to occur in various lithologies. They are known from chalk and limestones too. Lately, I studied many samples from limestones (and also shales and mudstones) deposited in warm shallow shelf waters near the equator and I have not found any dinocysts. There are no organic-walled cysts, no calcareous cysts, nanofossils are extremely rare. In contrast, many of the samples are full of beautifully preserved foraminifera as Globotruncana, Globigerina. I suspect too warm conditions (hampering dinoflagellate activity) or extremely high oxygen level (affecting the cyst preservation). I wonder if there could be any other explanation.
I'm carrying out a retrospective cohort clinical study,
Some patients have visited the hospital twice because of recurrence, with few months in between, but with different symptoms, complications, and cyst size in each time. Even the primary outcome is different in each visit.
What should I do?
Should I consider only the latest visit?
Or treat them like 2 different patients?
I am seeking information regarding dinoflagellate cysts from sediments. Is it relevant to sieve the cysts with 100 and 10 micron (specifically) mesh? If yes, ain't we be loosing a significant amount of cysts while sieving it via 10 micron mesh whose size falls in the range of 10 micron or little less.
Histopathology of a lesion (with provisional diagnosis of dentigerous cyst) showed cystic cavity lined by nonkeratinized stratified squamous epithelium. However connective tissue capsule showed daughter cyst with cystic lumen containing keratin flecks and lined by orthokeratinized epithelium. Very few inflammatory cells present.
I am trying to induce toxoplasma cyst formation (both RH and ME49) strains by altering the pH to 8.1 (DMEM, 1%FCS, 1% pen-strep), at 37oC, without CO2. However, the host cells weren't healthy and dying after 24 hours of incubation in condition without C02, any suggestion in improving this? As I understand that the change in media pH is the most common way to induce cyst formation in vitro. Thanks.
Cystic liver lesion mainly Hydatid liver cyst
I am working in the role of Wnt signal and spindle orientation.
I am working with mouse organoids from colon but I would like to have a simpler system to test some idea. In the lab we use CaCO2 cells that form 3d cyst but they are human cells.
I was wondering if someone have experience with mouse cells that can create cysts such as in CaCO2 cells.
Observed in Miocene brackish to freshwater sediments. Most probably siliceous. Any suggestions welcome. Thank you.
How is it possible these threads are now appearing? Can there be a correlation?
When preparing entomology labs for undergraduates, I studied cockroach hindgut endosymbionts extracted from excrements of Gromphadorhina portentosa. I found these very firm ‘cysts’ of ca. 0.5-1 mm long, with the thick transparent coverage. One ‘cyst’ has a special design (marked *). Do you have any idea what it could be?
The diagnose of Entamoeba histolytica stages in stool samples using microscopy most often give rise confusion of some cells like pus cells and other round cells which are resemble to cysts of Entamoeba histolytica, Moreover sometimes its difficult to the lab. technician to distinguish between the pseudopoda of this Entamoeba with other non-pathogenic rhizopoda, So I think this procedure is golden one to solve this lab mystery, why?
Oocysts, bradyzoites, and tachyzoites are the three infectious stages of T. gondii. Tissue cysts may develop in visceral organs, including the lungs, liver, and kidneys, they are more prevalent in the neural and muscular tissues, including the brain, eyes, and skeletal and cardiac muscles. Tissue cysts vary in size; young tissue cysts may be as small as 5μm in diameter and contain only two bradyzoites. While older ones may contain hundreds of organisms. Tissue cysts in the brain are often spheroidal and rarely reach a diameter of 70μm, whereas intramuscular cysts are elongated and may be 100μm long (Dubey et al,. 1998, Dubey, 2010). Transmission electron micrograph of two T. gondii tissue cysts in the brain of a mouse 6 months after infection with the Me-49 strain. The bradyzoites in the younger tissue cyst contain more micronemes and amylopectin than do those in the older tissue cyst. Additionally, the contents of rhoptries in the bradyzoites in the older tissue cyst are electron dense whereas those in the bradyzoites in the younger tissue cyst are honeycombed. The cyst wall (cw) also is more branched and prominent in the older tissue cyst than in the younger tissue cyst (Dubey et al,. 1998). The question is: What factors cause the size of the cysts in the brain to vary? Only time? Strains virulence？What causes the size of the cyst to be close and the size of the cyst to be significantly different?
I am trying to extract Gardia DNA from stool. What I succeeded to get so far is in range of 1000 bp which is far to short than published. Any suggestions?
Toxoplasma gondii can infect almost all mammals and form cysts in the muscles and nervous system of the host. The host can be infected T. gondii two or more times depending on the amount of infection and the virulence . The method of isolate T. gondii in the world is used, which was biological separation of cats and mice.
How to guarantee that there is only one type of T.gondii strain isolated, and whether the genotype found by RFLP-PCR is related to the isolates? Additionally, if Neospora and T. gondii are separated at the same time, how do you get their own strains and what technology is used?
I have looked everywhere for pictures of D. acuminata cysts, but have not found many references. I am looking for someone with experience in cyst identification to confirm/deny that either of these cysts are D. acuminata. Picture was taken at 20x. I can post more pictures if it would be helpful.
Patient's mother has ADPKD and suffered end stage renal disease at age 60. Patient's mother (and sister) first had onset of high blood pressure at age 30 and used ARB medications to control. The child shows onset of high blood pressure at age 17 and is set to begin blood pressure medications. Ultrasound shows cysts in both kidneys but normal size kidneys and normal function. Is the earlier age of onset of high blood pressure for the child any indication of potential for faster progressing disease and potentially earlier end stage renal?
Is any research points toward female prediction with suggestive pathogenesis for the same.
I want to get giardia trophozoites from stool cysts but reported methods I’ve tried doesn’t work. does anyone knows a method to obtain that?
As DNA isolated from quiagen blood & tissue kit, is giving OD value 0.80-1.4 at 260/230 where as the desired value is 1.9.
I have heard that the capture and selling of Artemia cysts might become prohibited because the species might enter the list of endangered species. Zebrafish facilities would have to adapt. Is this a myth?! Anyone have heard this?
What is the best method for detecting trophzoites and mature cysts of protozoan parasites in contaminated water?
I have a big interest in this field, since the number of PCOS woman is increasing now adays a lot, and a woman with a typical PCOS case suffers from obesity, providing them in the proper diet and sports plan can reduce the case but will surely not resolve it completely;
I already know that PCOS is treated by different methods of hormonal therapies which is not working in resolving the problem for many patients, and surgeries might be costly for some, a phobic idea for others and the surgery will never promise that the cysts wont grow again. I already heard from patients taking natural supplements of Myo-inositol ,D-Chiro-inositol and folic acid; the patients claim they felt a mild improvement maybe the number of cysts stoped increasing but the former ones are still existing.
Im waiting to be provided by a satisfying answer from experts in the field.
Thank you & Best Regards
Lower urinary symptom, especially the last 8 weeks. Needs a cathether to void urine.
It seem to origin from the urethra. Or?
I was planning a retrograde urethragraphy and urethra/cystoscopy
A 23 years old unmarried lady had reported during last week of August 2016 with C/O amenorrhea of approximately 55 days' duration. She used to have regular menstrual periods before that. Ultrasound of pelvis revealed complex cystic lesion in right adnexa ? Haemorrhagic cyst. She had normal periods on 01 Sep and 01 Oct. Repeat ultrasound on 14 Oct revealed complex rt adnexal cystic lesions. MRI pelvis was advised. Patient got MRI done on 10Nov. It shows rt adnexal complex cystic lesions showing septa and solid components. Contrast study has been suggested. She hasn't had periods after 01Oct. Otherwise, she is asymptomatic. How to go further in this case?
What is the natural history of these cysts and long-term outcomes of puncture and marsupialization?
Microscopic images (cross-section) of an ovarian cyst are shown in figures
can we find out the difference between cysts and tumors using a thermal image in the breast. If so how to find the difference between between the both radiations.
1. There are many protocols for freezing or preserving bacteria, but the concentration of glycerol varies from 10% to 80%, many scientists use different cryo protectants. Which is the most suitable preservation method and concentration of glycerol or composition of cryo protectant?
2. Azotobacter is cyst forming and a slow grower. How many hour’s Azotobacter culture is appropriate for cryopreservation?
Month ago I treated a 1 year old boy with a huge cervicomediastinal lymphangioma by aspiration the clear lymphatic fluid with subsequent injection into the cavity of Bleomycin and Triamcinolon. There were 4 bigger and many smaller cysts with propagation to the anterior mediastinum. Three days before the second stage the sclerosation therapy the outer cysts augmented and became harder without pain sensation. The sonography demonstrated these cysts with more tightly content unlike other (some of them no treated) cysts. There is no evidence of trauma or infection.
At the second attempt I aspirated by punction nearly 90 ml transparent homogenous fluid with dark red coloration. The US guidance indicated presence of many noncommunicating cysts. After application of 8 ml diluted Bleomycin I interrupted the manipulation by my own decision.
Now I intend to perform a first stage cervicotomy, and then a second stage thoracotomy in order to achieve radical extirpation of the lymphatic bundle. I have experience with cervicothoracic lymphangioma cases, executing in some of them one-stage cervicosternotomy. But this is a traumatic approach. Would you suggest another tactic?
Carnoys solutions as adjuvant therapy to fix and kill a remnant epithelial cells ,daughter cyst and microcysts as chemical cauterization agent . Some surgeons used it preoperatively whereas others use it postsurgically over surgical site.
During treatment of KCOT using either enucleation with or without ajuvant therapy or marsupialization with or without residual cystectomy, some authors advocate that excision of the overlying mucosa is necessary to eliminate epithelial and micro cysts with subsequent reducing recurrence?
- Which parameters (heavy metals, fatty acids, amino acids, etc.) can be identified Artemia cysts collected from different lakes.(To resolve disputes on poaching cysts).
- How long can main part of dormant Artemia cysts stay alive at the bottom of the lake? Are dormant cysts decomposed by microbes?
How many Artemia cysts are harvested in the world or in a country?
How many cysts should we harvest for aquaculture goals? How many cysts of Artemia consumed per year in the world or in a country?
- Artemia parthenogenica is a parthenogenic brine shrimp , a crustacean
I would like to collect Artemia parthenogenitica cysts. Could Somebody suggest me the source ?
We isolated some Acanthamoeba cysts after filtering of water samples, we need its DNA for PCR but there is some difficulties for breaking the cysts wall after using all physical & chemical usual methods.
Are these sample cysts? I incubated the live single cyst however were not germinated yet.
are there correlation about umbilical cord cyst and trisomy 18?
Umbilical cord cyst from prenatal detection what should be done?
I found a strange histologic finding of acantholytic dyskeratosis in the lining epithelium of epidermal inclusion cyst, which is more commonly found in acantholytic dermatitis such as Darier's disease, Warty dyskeratoma etc.
I found this pathologic feature might suggest a diagnosis of familial dyskeratotic comedons or something like that. But there is no familial tendency in this patient (30yrs-old male) but only a few histories that he got surgical removals of epidermal inclusion cysts over a period from another site of his body.
Is there any other disease that I should differential diagnose in this case?
Can the cyst being broken by liquid Nitrogen?
Well, they give the name to the syndrome – but are they really central to the clinical presentation? The current definition allows a patient to have PCOS without the presence of PCO. Affected patients suffer from hyperandrogenism – hirsutism, acne and alopecia – which is linked to hyperandrogenemia: Is there solid data to support the notion that patients with PCO more often have hyperandrogenism or are more severely affected? PCOS also presents with a- or oligomenorrhea – which is linked to anovulation and thus reduced fertility. Wedge resection of the ovaries or other techniques to reduce the number of cysts = follicles like ovarian drilling improves fertility – does that mean the reduction of “cysts” is the cure or is this rather a stimulant in itself or the result of the concomitant reduction of androgens? Also, PCO does not influence the metabolic consequences of PCOS, such as insulin resistance, glucose intolerance or obesity. Apparently, PCOS may even be defined without PCO, as the 1990 NIH definition did – or was this merely an expression of lesser emphasis on ultrasound in the USA than in Europe? What does the inclusion of PCO in the 2003 Rotterdam definition improve, other than widening the spectrum of patients, some of which do not even have a relevant clinical problem (e.g. PCO plus hyperandrogenemia without hyperandrogenism)? How do we really define PCO: The number of “cysts” per ovary to separate “true” PCO from healthy women with multiple follicles has increased from 10 to 12, and recently to over 25  – how is that practical in routine work? Earlier  and recent work  suggests that PCO morphology does not associate with significant consequences for health in the absence of other symptoms of PCOS – why then should we bother and why should we not opt for a better name for the syndrome?
 Dewailly D et al. Definition and significance of polycystic ovarian morphology: a task force report from the Androgen Excess and Polycystic Ovary Syndrome Society. Human Reproduction Update 20:334–352, 2014
 Legro RS et al. Polycystic Ovaries Are Common in Women with Hyperandrogenic Chronic Anovulation but Do Not Predict Metabolic or Reproductive Phenotype. J Clin Endocrinol Metab 90: 2571–2579, 2005
I need publication recent about this and thank you all of you.
Endometrial cyst is mostly an age related syndrome, seen mostly in mares over 10 years old. The current treatment either not practical (like laser, endoscope) or not efficient. We usually used in our clinic a hypertonic solution (Nacl), but the recovery rate is not encouraged. I am seeking a simple practical method to overcome this problem.
I need to quantify larval stages of Taenia pisiformis found in the viscera but I can not find references about this. Thanks
Here is a large hydatid cyst of the kidney and only a small part of the kidney is remaining, which is flattened in the internal upper part of the cyst.
With only this assessment, will you recommend nephrectomy or nephron-sparing surgery.
In spinal echinococcosis a complete removal of the cysts is not always possible. Some authors recommend treating the site with hyperosmolar solutions in an attempt to damage and eliminate the rest of minor cysts. In my opinion the efficiency of similar approach depends upon the type of echinococcus. Sometimes it may cause spreading of scolices?
Why are single enhanced lesions common among Indians whereas multiple cysts are found in western countries?
I understand that the cysts are difficult to eradicate, but do they cause an immune response, or is the immune response caused by the free tachyzoites?
commercially available IFA kit for E. histolytica cysts