Science topics: AngiospermsZingiberalesZingiberaceaeCurcuma
Curcuma - Science topic
A plant genus of the family ZINGIBERACEAE that contains CURCUMIN and curcuminoids.
Questions related to Curcuma
For my experiment, I am trying to observe the growth of two gut microbes against in vitro agar substances which represent dietary fibre (turmeric, golden seal, and citric acid) and fats (palmitic acid, stearic acid, and yeast).
i plan to do this using a spread plate method which would have my substances mixed in agar and the bacterial culture spread on top.
i won't be using different concentrations, I will be using recommended daily intake for each substance.
my problem lies as I want to have a statistical analysis. I am already using CFU technique but I would also like to measure the optical density of my substances for bacteria growth.
How would I go from my various petri dish cultures to a spectrometer to analyze the absorbance?
would I have to perform two tests? using a petri dish for CFU and a liquid culture for optical density?
isolation of rhizobacteria from turmeric rhizomes but there is the contamination of fungus.
Hello everyone. I am currently working on comparing antioxidant potency between my samples.
I have 4 samples = water kefir, water kefir infused with butterfly pea, water kefir infused with turmeric and water kefir infused with matcha green tea.
As of for now, I am comparing them on the basis of how they fare in the two assays I have done; DPPH & FRAP assays. However, I am doubtful of this comparison method as they're not exactly comparable since they have different unit and measurement.
I have recently encountered the concept of relative antioxidant capacity index (RACI) from few recently published papers. However, I am still not clear on how could I integrate this concept for my result.
Could anyone provide me any insight on how does one use this concept to compare between antioxidant potency of different samples?
Hello everyone. I need help regarding my DPPH assay.
I'm currently trying to measure the Antioxidant Activity of my sample, Water Kefir infused with Plant extract (Turmeric and Green Tea) using DPPH method.
My DPPH Assay protocols are as follows :
A DPPH stock solution was prepared by mixing 24 mg DPPH in 100 mL Methanol. The working solution was obtained by mixing 10 mL stock solution in 45 mL Methanol to obtain an absorbance of 1.1 0.05 units at 515 nm using ELISA plate reader (Bio-tek Instruments, USA). 50 µL of sample was allowed to react with 250 µL DPPH working solution in dark for 30 minutes. The absorbance was then read at 515 nm using ELISA plate reader (Bio-tek Instruments, USA). Trolox solution was used as positive control in this experiment.
However, I noticed that there is precipitate/sediment formed after I incubated my samples for 30 minutes.
Does anyone know what might be the possible cause and how can I minimize the occurrence of this precipitate/sediment?
Also, if I aliquot the supernatant to a new plate and get the absorbance reading, does it affect my result somehow?
I also attached this photo of my plate to give you a better idea on how does the precipitate/sediment looks like
Note : I serially diluted my samples (2 fold each, 6 times). I noticed that the higher the concentration, the more likely the precipitate/sediment to form (A = 1mL, B = 0.5 mL, C = 0.25 mL, D = 0.125 mL etc)
Few spices or herbs are claimed to be effective in corona prevention/ treatment such as Pepper, Turmeric, Ginger etc. How do they affect ACE2 receptors in lab tests/ animal/ human tests?
I am working on isolation of PGPR from the turmeric plant but fungal growth contaminates and bacterial isolation is not done properly.
I am doing my Ph.D. research on Agrivoltaic system in Odisha, India. Experimental work has been completed with good performance indicators values but now needs simulation results of Turmeric plant growth w.r.t. solar light/radiation and Solar panel output w.r.t. solar light/radiation.
Official email: firstname.lastname@example.org
Most of the open literature explains that the QuEChERS followed by dSPEmethod may be suitable, but from my practical experience, it was found that it is not suitable for such types (spices) highly complex matrices. The scientific community in the field of Foods and Beverages, please come up with a fresh concept on this topic....
In my study on Awassi sheep, it was concluded that adding turmeric to the ration led to diverting the path of fat accumulation from the parts of the carcass to the broad tail, with significant weight increases in the treated lambs.
We have screened the phytochemicals of Tinospora cardifolia and curcuma longa downloaded from Imppat database and Dr. Dukes database and targeted TGFbeta-R1 crystal structure using docking study and found one best compound from each quercetin from c.longa and tembetarine from T.cardifolia , Inorder ro test the compound to test invitro & invivo, we wish to purchase from chemical vendors but we found the compounds source from other plant species (Apis mellifera) but structure remains same for both cases. Is it a valid study if we conduct a study based on this
link : Quercetin ≥95% (HPLC), solid | 117-39-5 (sigmaaldrich.com)
My farm location has 800 mm rainfall. Soil is deep black soil. Ph:8-8.5 Turmeric is a 9 months crop. I have no drip facility. And I can give irrigation once in every month from November to March. Sowing is done in July last week. Another 600 mm rainfall is expected during August to November, max. being in August, September and October, with ~30, 50, 20 ratio. If I use mulch I cant use surface irrigation. And rainfall will be probably lost through surface runoff. However, mulch conserves soil moisture, keeps water losses away from weeds and minimizes weed load, reduces soil temperatures. What is the possible production scenario?
Can turmeric be used as a substitute for the other comercially availble antifungal agents during plant tissue culture?
In mutation breeding with EMS, turmeric single bud chips were used. Near LD50, many sprouts started withering. But due to care in handling like helping the pseudostem to open, helping the leaves unfold in budlings, helped more budlings to survive. Further, some of sprouts withered and died due to deleterious mutations, but new sprout arose from the bud, in the nodes (generally in the opposite to original sprout). These new sprouts were given extra care to survive, by placing them at proper position in the protrays.
1. Helping pseudostem to open freely.
2. Helping budlings to open leaves freely while germinating so that budling can establish.
3. Allowing the new sprout to grow in withered budlings, by placing the new sprout at geotropically advantageous position.
All these measures helped to achieve recovery of more mutant population.
Any opinion on the utility of this method in mutation breeding.
Turmeric is a polyploid. Mostly sterile. However, it sets seed occassionally. I have collected seed and kept in poretrays filled with cocopeat. I have not observed any germination even after 15 days. Yesterday I have kept some seed on germination paper spread in a petridish. Can any one tell me what is the viability and time taken for germination of turmeric seed. Seed was collectd from inflorescences fifteen days ago, the flowering was complete about five months ago. Seeds were within the inflorecences for about five to six months before collection and sowing.
We are trying to estimate curcumin content in Turmeric genotypes. Rhizomes are cut in pieces and oven dried at 60 o C. Certain genotypes retained the original colour on cut surfaces and others changed to brown to dark brown on cut surfaces. What is the reason? Does polyphenol play a role ? What about other factors? We are sure that it is not due to excessive heat, as we have seen this even under lower temperatures.
Currently, I am working on the antioxidant property and phenolic content of various species of Curcuma using the MAE method taking methanol as a solvent. What factors should I focus on for accessing a fine result from the extraction method?
what all standards are needed to quantify and analyze the composition with a proper method fie for the mobile phase to run the sample ?
I am talking about the GI tagged Kandhamal Haladi (Turmeric), which is famous for its curcumin content.There are few blocks in Kandhamal district such as Balliguda where curcumin content is very low. I want to know the points where the post harvest loss of curcumin takes place.
Turmeric is a polyploid and vegetatively propagated crop. Usually, F1 populations are used to identify QTLs. As this crop generally has no viable seed, what is the best approach to genarate QTLs.
I got permanent relief from cough cold running water nose bronchitis and thi nitis by drinking herbal turmeric added milk boiled with a mixture powder of black pepper clove con namon and fried zonder power in viron time.
You can also try this miracle.
I'm having difficulty recovering curcumin from my ethanolic extract. I tried reducing its solubility in the solution by slowly adding water, but the solid just crashed out of the solution in very fine particles and I'm unable to filter it out. I also read that the presence of oils in the extract make it very difficult to crystallize the curcumin, so I tried removing the oils from the turmeric with hexane first before extracting with ethanol.
Does anyone know of better and more efficient ways of recovering curcumin from the solution?
As I learned from various material, it seems regular consumption of turmeric approx 3 to 5 grams create an good impact to human bodies, I would like to know the amount of daily consumption of 100 % Pure curcumin which contribute an effect to improve metabolism activity and immunity power.
Assuming 5% usage of Black pepper increases the bio availability of curcumin in body.
I intend to perform an ethnographic study on Indian or Sri Lankan traditional practices (mostly in Hindus methodology) in treating chickenpox, focusing on using "Neem", "Turmeric" in particular. Culturally many practices are highly irrelevant to modern science and superstitious, some practices seem to be effective.
Please assist me by offering your viewpoints on this
UPDATE: The recent evidence I could see indicates the opposite. Curcumin and Ginger may help against COVID-19. But we need further research.
I understand that COVID-19 thrives and causes infection when ACE2 is expressed.
I found some papers indicating selenium, curcumin and ginger inhibits ACE, thus leading to increased expression of ACE2.
Should we conclude that taking ginger / turmeric extract (curcumin) supplements (liquid or powder) may increase the risk of COVID-19 infection? Paper 7 seems to be contradictory but I could not understand it well.
My understanding is they make us vulnerable for catching the infection but when we are infected, they can help with their anti-inflammatory mechanism to reduce cytokine storms, thus leading to survival.
- Paper 1 indicates: "ACE inhibitors actually INCREASE expression of ACE2"
- Paper 2 indicates "Among phytochemicals, curcumin treatment significantly inhibited the concentration and activity of ACE, concentration of AngII, and mRNA expression of ACE
- Paper 3 indicates "Curcumin is known to have no significant difference in inhibiting ACE compared to Captopril, but when it was incorporated in the self-nanoemulsifying carrier, it slightly increased the inhibitory effect on ACE."
- Paper 4 indicates "It is noteworthy that treatment with dietary curcumin inhibits oxidative stress and inflammation, as we have reported previously. Moreover, curcumin attenuates fibroblast proliferation and TGFβ1/Smads expression, as noted in the present study; this action may concomitantly attenuate reactive myocardial fibrosis via a pressure-dependent or -independent pathway. We found that dietary treatment with cur-cumin reduced expression of the AT1 receptor and enhanced expression of both the AT2 receptor and ACE2. These results suggest that curcumin modulates not only Ang II/AT1/AT2 receptor-dependent signaling pathways, but also activates an ACE2-mediated mechanism that modulates myocardial fibrosis. "
- Paper 5 indicates: "Treatment with curcumin suppressed ACE expression in TAA liver and reversed the toxicity produced"
- Paper 6 indicates: "Ginger and turmeric rhizomes are used in folk medicine for the treatment of hypertension but the mechanism remains unclear. Pre-treatment with both rhizomes respectively caused a significant decrease in ACE and arginase activities with a concomitant increase in nitric oxide (NO) level.Dietary supplementation of ginger and turmeric rhizomes inhibited ACE and arginase activities as well as increased NO production in L-NAME induced hypertensive rats. These activities could suggest possible mechanism of action for their antihypertensive benefits in traditional medicine"
- Paper 7 indicates: "we suggested that nelfinavir and lopinavir may represent potential treatment options, and kaempferol, quercetin, luteolin-7-glucoside, demethoxycurcumin, naringenin, apigenin-7-glucoside, oleuropein, curcumin, catechin, and epicatechin-gallate were the most recommended compounds found in medicinal plants that may act as potential inhibitors of COVID-19 Mpro".
Turmeric is a golden spice famous for its culinary use in Asian cuisine. Its use has not been limited to lucrative food preparation since antiquity. Curcumin, one of the highly valued natural compounds, underlies the use of turmeric-based formula in traditional medicine. Can we find any systemic area which lacks curcumin-directed traditional knowledge or scientific evidence of curcumin-based research? So, why this nature's gift still could not find its way to conventional medicine?
Now I'm looking at turmeric powder and its effect on the production of milk and its components in ewes
The genomic DNA,I isolated from turmeric using ctab .The protocol followed for genomicDNA isolation is: grind turmeric leaves using DNA extraction buffer(100 mM tris PH 8,20 mM EDTA,1.4 M Nacl,2% CTAB)and PVP without using liquid nitrogen,then incubate at 65 degree celsius for 1 hour,followed by two times extraction with chloroform and isoamyl alcohol and addition of isopropanol with overnight incubation.This is followed by centrifugation,ethanol wash of pellet.The dried pellet is finally dissolved in nuclease free water.The absorbance value AD260/280 of DNA is around 2-2.3.The PCR done using this DNA is not giving bands in most cases using ssr primers.I doubt it's due to DNA quality.Can someone help me out with the DNA isolation protocol for turmeric?
I've been researching possible manners of combat cancer and I got an idea that I would like to share with you to receive feedback.
My idea consists of making a nanoparticle in which nucleus contains curcumin, which is the principal antitumoral agent of the Curcuma. What I think of this nanoparticle is to function with a PH measurer that at the moment of getting into a place where is more acid the particle break and the curcumin can be spread.
I hope to receive feedback from you, thank you very much.
Working on genetic diversity analysis of turmeric varieties using ssr primers.some ssr primers do not give bands at all on gel for some varieties.why this is so?is it because of primer or dna quality of turmeric samples.
I have read that turmeric inhibits telomerase. I have also heard telomerase helps repair telomeres. Does this mean turmeric stops telomeres repairing or have I heard wrong?
Also mention media, pH, temperature and other conditions required for the isolation.
Please provide the names of some of the plant sources of curcumin, other than Curcuma Genus.
Is turmeric a root crop or tuber crop?
which nutrient could be responsible for higher curcumin percentage and essential oil in turmeric?
I found health line article that explains that black tea can be present with toxins so I was just curious about that.
The only possibility that I do know where that comes from is the soil within that location where minerals are present.Just for a quick review.
I need to know for the presence of food safety precautions and the toxicity found in ginger, turmeric and lemongrass.
I'm dealing with this risky bacteria.
I want to analyze a compound which is polar in nature and coexists in a colored solution of turmeric (curcuminoids). I want to remove the colors keeping intact the polar molecule present in the solution so that I can get clean chromatogram while injecting the sample.
How to remove the coloring agents from the reaction mixture keeping intact the target polar molecule? If any adsorbent to be used which one would fulfill this purpose?
Your answer will be highly appreciated. Thank you.
Hi, I need to analyse Total Flavonoid Content in a crude leaf extract. I notice Potassium Acetate is one of the reagents involved whenever Quercetin being used as standard compound. In the event where Rutin being used as standard , I notice Potassium Acetate is not involved.
Question 1 :May I know if Potassium Acetate seriously required for Total Flavonoid Content determination ?
Question 2 : Is it acceptable to use Quercetin as standard without having Potassium Acetate in the reagent list ?
Question 3 : I am making references from attached papers. Would like to know if method for Total Flavonoid Content used in paper titled "Screening of Total Phenolic and Flavonoid Content in Conventional and Non-conventional Species of Curcuma." generally accepted ?
In the research turmeric (in initial step) should be solved in a solvent and ethanol was described as a solvent with non-toxic and non-irritative characteristics. What side effects could ethanol contribute to the experiment?
I'm testing antimicrobial inhibition on Candida Albicans using fluconazole drug in a powder form and I'm worried that if i use DMSO or Ethanol as a solvent, it will be toxic and affect the yeast growth. Also I need to extract turmeric and goldenseal as plant extracts to test antimicrobial activity on Candida and I was wondering if i should use ethanol for the extraction or can I use sterile water.
How to do calibration curve for Curcuma longa?
To know how much active compound in it.
Can anyone provide me with the list of Curcuma species occurring in India (including new species)?. I could only find around 44 species.
Ginger and turmeric being vegetatively propagated have high risk for transmission of several diseases which could be lethal and debilitating. Use of poor quality planting material adversely affects the production, productivity and quality of rhizomes.
1. Seed rhizomes if infected may carry load of degenerative diseases. How we can ensure freeness from diseases.
2. How diagnostics can be used in the system.
3. Seed rhizomes are prone to heavy post harvest losses and we need to store till new season comes. In this case what could be the ideal storage conditions?
4. What should be large scale quality planting material production chain for high yielding varieties.
Curcumin is a compound of natural materials including group what?
I loaded curcumin on graphene oxide that did not work! I took uv-vis but the result was not correct.I think maybe the problem is the solvent. I want to know what is the best disperser for both grapheme oxide and curcumin?
Many studies showed high dose of curcumin induces ROS formation and caspase medicated apoptosis in both fibroblasts and keratinocytes which should in theory make curcumin unfit for the treatment of cutaneous wound healing. The curcumin I have always known was cytoprotective, antioxidant, anti-inflammatory and has potent wound healing ability but these study results prove otherwise.I know Curcumin interacts with multiple pathways and has multiple pharmacological properties, so some how It might pleiotropically promote wound healing besides its cytotoxic effect on these cells.but I am not sure how inhibitory effect of curcumin on fibroblast and keratinocytes proliferation could help with cutaneous wound healing.
1) Aloe vera 2) Cynodon Dactylon 3) Curcuma Longa i.e turmeric 4) Althaea officinalis i.e marsh mallow 5) Achillea millefolium i.e yarrow
Please help me from your experiences
Curcuma amada represents a major source of valuable drugs, pigments and phytochemicals, the major chemical components of Amada include phenolic acids, volatile oils, starch, curcuminoids and terpenoids. I am searching for the pure compound Amadaldehyde for UV vis study. Could anyone help and give me suggestions regarding availability of Standard Amadaldehyde.
Curcumin is soluble in water with alkaline conditions, but its color will change to red. This discoloration is due to changes in pH. Do its effects (antimicrobial,...) change in alkaline condition?
Curcumin is soluble in alkaline water. What is the main reason for the red color in alkaline condition?
I am performing plant DNA barcoding using curcuma genus as material. I am analyzing the sequence now but don't have much on an idea how to evaluate the barcode. I am using Mega software. Can anyone share their experience?