Science topic

Culture Techniques - Science topic

Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
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Publications related to Culture Techniques (10,000)
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Campylobacter infection is a serious health problem worldwide. There is still insufficient information in Bulgaria about the spread and course of the infection. Data on campylobacteriosis in adults are particularly scarce. In our study, we performed a comparative analysis in patients in different age groups, including the resistance of isolated str...
Article
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Organoid cell culture methodologies are enabling the generation of cell models from healthy and diseased tissue. Patient-derived cancer organoids that recapitulate the genetic and histopathological diversity of patient tumours are being systematically generated, providing an opportunity to investigate new cancer biology and therapeutic approaches....
Article
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Yadav D, Adhikari A, Dhuingana B, Gurung H, Khatri N, Pandit S. 2022. In-vitro efficacy of Trichoderma isolates on Sclerotium rolfsii causing collar rot of chili. Asian J Agric 6: 97-102. The experiment was conducted in the Nepal polytechnic institute plant pathology laboratory to study the in-vitro efficacy of Trichoderma isolates on Sclerotium ro...
Conference Paper
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Abstract: Molecular virology laboratories are crucial in a pandemic crisis because they define our awareness by boosting our ability to discover and identify about new viruses and their linkages to specific diseases before viral infections cause widespread morbidity and mortality. In critical occasion, such as COVID-19 pandemic, right time diagnose...
Article
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Poster session 2, September 22, 2022, 12:30 PM - 1:30 PM Objectives The presence of Candida species in the urine (Candiduria) is a typical clinical observation, especially in hospitalized patients. It is more common in immunocompromised persons, in bone marrow transplant unit (BMTU) patients, and in those who are in intensive care units (ICUs). Ur...
Article
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Objective Imaging endothelial cell behaviour under physiological conditions, particularly those associated with chronic fibrotic pathologies, is an incredibly challenging endeavour. While short-term assessments (hours) can be achieved with techniques such as intravital microscopy, vascular changes often occur over days and weeks which is unfeasible...
Article
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Micronutrients are essential for plants. Their growth, productivity and reproduction are directly influenced by the supply of micronutrients. Currently, there are eight trace elements considered to be essential for higher plants: Fe, Zn, Mn, Cu, Ni, B, Mo, and Cl. Possibly, other essential elements could be discovered because of recent advances in...
Article
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Cell wall deficient bacterial L-forms are induced by exposure to cell wall-targeting antibiotics and immune effectors such as lysozyme. L-forms of different bacteria (including Escherichia coli) have been reported in human infections, but whether this is a normal adaptive strategy or simply an artifact of antibiotic treatment in certain bacterial s...
Article
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Morphological and molecular characterization of 10 isolates of an antagonistic fungi as Trichoderma viride. Antagonistic properties were being tested by means of dual culture technique against Fusarium oxysporum f,sp. lentis, causing wild disease in lentil crop. One of the isolate (68 L) was found to be most potential antagonistic against the cause...
Article
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The experiment was conducted in plant pathology laboratory of Nepal polytechnic institute for studying in invitro efficacy of Trichoderma isolates on Sclerotinia sclerotiorum causing white mold of kidney bean, Bharatpur, Chitwan, Nepal by dual culture technique. The experiment was carried out in completely randomized design (CRD) with four replicat...
Article
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In vitro culture techniques, using spores, were used to propagate the critically endangered fern Polystichum drepanum, in order to reinforce its known natural populations. In 1998 and 2010, a total of 194 plants of P. drepanum were reintroduced at Ribeira do Inferno, a historical habitat of this species and the source of the spores used in this res...
Article
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Malaria elimination includes neglected human malaria parasites Plasmodium vivax, Plasmodium ovale spp., and Plasmodium malariae. Biological features such as association with low-density infection and the formation of hypnozoites responsible for relapse make their elimination challenging. Studies on these parasites rely primarily on clinical samples...
Article
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During the present study, the antagonistic potential of Trichoderma harzianum Rifai against Phytophthora infestans (Mont.) de Bary and Rhizoctonia solani Ktihn. was evaluated by dual culture technique. Its efficacy to inhibit pathogenic effects caused by these two pathogens was also evaluated on tomato and potato, under artificial inoculation condi...
Article
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Introduction Mesenchymal stromal cells (MSCs) release extracellular vesicles (MSC-EVs) containing various cargoes. Although MSC-EVs show significant therapeutic effects, the low production of EVs in MSCs hinders MSC-EV-mediated therapeutic development. Objectives Here, we developed an advanced three-dimensional (a3D) dynamic culture technique with...
Preprint
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Researchers have recognized that culture dimensions are essential in language learning and effective communication. The present study aims to investigate teachers' attitudes toward culture-based classes in the context of English language teaching. Particularly, this paper will probe EFL teachers' perceptions on the purposes targeted in their cultur...
Article
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As a counter to climate change, energy crises, and global warming, microalgal biomass has gained a lot of interest as a sustainable and environmentally favorable biofuel feedstock. Micro-algal carbohydrate is considered one of the promising feedstocks for biofuel produced via the bio-conversion route under a biorefinery system. However, the present...
Article
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Aflatoxin contamination poses serious health concerns to consumers of peanut and peanut products. This study aimed at investigating the response of peanuts to Aspergillus flavus infection and aflatoxin accumulation. Isolates of A. flavus were characterised either as aflatoxigenic or nonaflatoxigenic using multiple cultural techniques. The selected...
Article
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Black leaf spot caused by Alternaria brassicicola is one of the destructive diseases of crucifers and causes considerable loss in terms of quantity and quality. The yield loss due to Alternaria was 5-30% in the entire cabbage growing areas of India. Hence, experiments were conducted to determine the efficacy of fungicides and bioagents both in vitr...
Article
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During the last few decades, aquaculture as a useful animal protein providing sector intensifies to meet the growing world population demands. Although fish culture technological advancement has resulted in increased production, intensive technology has negatively affected the environment-consequently, aquaculture research efforts have been diverte...
Article
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Disease modeling using human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) has both challenges and promise. While patient-derived iPSC-CMs provide a unique opportunity for disease modeling with isogenic cells, the challenge is that these cells still demonstrate distinct properties which make it functionally less akin to adult car...
Article
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For the development of improved strain(s) of Calocybe spp. with desirable traits, interspecific hybridization of Milky white mushroom (C. indica) and St. George's mushroom (C. gambosa) was done by intermating their single spore cultures. In vitro evaluation of both the species for mycelial characteristics, growth rate, colony colour, days taken for...
Article
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Symbiotic nitrogen fixing bacteria comprise of diverse species associated with the root nodules of leguminous plants. Using an appropriate taxonomic method to confirm the identity of superior and elite strains to fix nitrogen in legume crops can improve sustainable global food and nutrition security. The current review describes taxonomic methods p...
Article
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Major carps, native freshwater fish in South Asian nations, are economically valuable fish species with high market demand. Coastal rural people can cultivate them in their own seasonal, largely underutilized, small homestead ponds with low input and understanding. However, the major problems with fish production in this region are the lack of qual...
Article
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Objective To systematically evaluate the literature on the therapeutic use of Schwann cells (SC) in the repair of peripheral nerve injuries. Methods The Cochrane Library and PubMed databases were searched using terms [(“peripheral nerve injury” AND “Schwann cell” AND “regeneration”) OR (“peripheral nerve injuries”)]. Studies published from 2008 to...
Book
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The freshwater apple snail, Pila globosa is one of the most abundant and commercially valued molluscs. The present condition of P. globose in south west region is alarming due to indiscriminate killing for Prawn farming. The present study was aimed at elucidating the breeding behavior and reproduction process of P. globosa. The natural breeding exp...
Article
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Indiscriminate defecation in open places is still a common practice in Agbani, Nkanu West Local Government Area, and Enugu State Nigeria. It is obvious that the practice does not only pollute the environment but also some of these people that defecate in the open places may be carriers of infections and may deposit enteric pathogens into the agricu...
Article
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Background: Rice brown leaf spot is a major fungal disease caused by Bipolaris oryzae. Biological management of brown leaf spot can be achieved by bacteria associated with phyllosphere region of rice crop. Methods: Antagonistic bacteria were isolated from phylloplane of the rice crop using dilution method. Phylloplane bacteria were morphologically...
Article
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This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. Abstract Helicobact...
Article
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Bestiolina amoyensis distributes in subtropical inshore waters across the Pacific Ocean, with a relatively long reproductive lifespan and high intrinsic population increase rate compared with other small paracalanid species, which makes it a good candidate to develop culture techniques for hatchery larval rearing. However, the reproductive biology...
Article
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Background Stemness of CD133 ⁺ EPCAM ⁺ hepatocellular carcinoma cells ensures cancer resistance to apoptosis,which is a challenge to current liver cancer treatments. In this study, we evaluated the tumorcidal activity of a novel nanoparticle of nitidine chloride (TPGS-FA/NC, TPGS-FA: folic acid modified D-α-tocopheryl polyethylene glycol 1000 succi...
Article
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Endothelial progenitor cells (EPCs) are currently being studied as candidate cell sources for revascularization strategies. Despite these promising results, widespread clinical acceptance of EPCs for clinical therapies remains hampered by several challenges. The challenges and issues surrounding the use of EPCs and the current paradigm being develo...
Conference Paper
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L’exposition actuelle des sociétés au risque climatique est en grande partie due aux évolutions de notre culture technique. L’ergonomie a d’ailleurs rencontré des difficultés pour atténuer l’impact de cette culture technique (automatisation, spécialisation etc.) sur le bien-être. Les low-tech (ou basses technologies) proposent de reconfigurer notre...
Article
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Esterases represent a class of hydrolases, and are widely distributed in animals, plants and microorganisms. Depending on whether they hydrolyze small water-soluble esters or ester bonds of long-chain acylglycerols, they are classified as carboxylesterases (EC 3.1.1.1) or lipases (EC 3.1.1.3). A host of novel microbial carboxylesterases from cultur...
Article
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Muhammad FN, Rizali A, Rahardjo BT. 2022. Diversity and species composition of ants at coffee agroforestry systems in East Java, Indonesia: Effect of habitat condition and landscape composition. Biodiversitas 23: 3318-3326. Habitat condition and landscape composition are the main factors that shape biodiversity in agroecosystems. However, informati...
Article
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Integrated multi-trophic aquaculture (IMTA) has the potential to enhance growth, reduce nutrient loads, and mitigate environmental conditions compared to traditional single-species culture techniques. The goal of this project was to develop a land-based system for the integrated culture of seaweeds and shellfish, to test the efficacy of integrated...
Article
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Dans la lignée de travaux sur le genre des techniques, nous nous intéressons dans cet article à la possibilité d’une revalorisation sociale des pratiques féminines de travaux d’aiguille, par l’intermédiaire des espaces en ligne qui leur sont dédiés. En nous appuyant sur une enquête par entretiens semi-directifs menée auprès d’utilisatrices de la pl...
Article
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Purpose: Helicobacter pylori has been implicated in most severe cases of peptic ulcer. Despite this development, the diagnosis of this infection has been a major challenge due to the difficulty encountered during isolation using the conventional culture method. Presently, most health facilities in rural communities in Nigeria still adopt the cultur...
Article
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Three-dimensional (3D) tumor culture techniques are gaining popularity as in vitro models of tumoral tissue analogues. Despite the widespread interest, need, and present-day effort, most of the 3D tumor culturing methodologies have not gone beyond the inventors’ laboratories. This, in turn, limits their applicability and standardization. In this st...
Conference Paper
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The three-dimensional (3D) culture of cancer cells has emerged as a better model resembling the in vivo tumor microenvironment due to the preservation of cellular morphology and interactions within the mass. Encapsulation of living cells in calcium alginate beads provides a biocompatible environment that holds the cells within the matrix. The ma...
Article
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Background and Objective: Aloe vera (L.) Burm. f. is one of the most vital medicinal plants due to its diverse healing capabilities. Aloe vera leaf disease is a source of concern due to its economic value. An experiment was carried out to discover the fungal pathogen associated with Aloe vera leaves to evaluate the effect of various physical factor...
Article
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Objectives This study was aimed to investigate the association between virulence factors and antifungal susceptibility pattern among Aspergillus species. Materials and Methods This study was carried out in the Department of Microbiology, from May 2018 to June 2019. A total of 52 Aspergillus isolates obtained from various clinical samples were speci...
Article
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Salmonella spp are characterized as rod- shaped, motile, gram- negative bacteria which has the ability to infect animals and human. Salmonella spp occasionally causes sickness while in most cases not lead to severe symptoms. Analyzing milk for Salmonella spp. is not routine but traditional culture methods are used to evaluate the health condition o...
Research
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Keywords: ABSTRACT Fusarium solani, Trichoderma harzianum, Carbendazim On isolation, from diseased plants, the associated pathogen was identified as Fusarium solani and its pathogenicity was confirmed on susceptible cultivar RGC 197 by seed, soil and seed-cum-soil inoculations under artificially inoculated conditions. Comparative efficacy of fungic...
Article
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The human gut microbiome is acknowledged as being associated with homeostasis and the pathogenesis of several diseases. Conventional culture techniques are limited in that they cannot culture the commensals; however, next-generation sequencing has facilitated the discovery of the diverse and delicate microbial relationship in body sites and blood....
Chapter
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Microalgae are the primary producers on the earth. They are ubiquitous. They have vast applications in science ranging from biotechnological applications, environmental applications, and pharmaceutical industries. Commencement of phycological research requires isolation of most promising algal strains with correct morpho-taxonomic identification. O...
Article
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Background: Accurate quantitative analysis of microorganisms is recognized as an essential tool for gauging safety and quality in microbiology settings in a wide range of fields. The enumeration process of viable microorganisms via traditional culturing techniques are methodically convenient and cost-effective, conferring high applicability worldw...
Article
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Vermicompost is nutrient-rich casts generated by the earthworms that can be used as biofertilizers. Since the potential of fabricating vermicompost varies amongst species, the present study was conducted to determine the vermicomposting efficiency of local earthworm species of Darjeeling hills and Eisenia fetida, that was subjected to three differe...
Article
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Patients undergoing implant treatment are at risk of peri-implant bone loss, which is most often caused by the adverse effects of microorganisms, but there are few proven procedures for their reduction. The aim of the research was to identify the microorganisms inhabiting the composites used to close the screw access hole (SAH), compare them numeri...
Article
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Objective: To evaluate the effect of different stocking densities:12 (D12), 18 (D18) and 24 (D24) individuals m-2 (ind m-2) on growth (weight and length) and survival in juvenile river crocodiles (Crocodylus acutus). Design/methodology/approach: The crocodiles were cultured in each treatment by triplicate for 43-d in nine plastic tanks and fed a di...
Book
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Que font les personnes, les corps et les techniques en termes de dynamique de genre ? En quoi et comment les personnes et les corps instaurent-ils, avec les techniques, de nouvelles relations de genre ? Que fabriquent véritablement les corps en fonction de leurs assignations respectives et de quelle latitude les personnes disposent-elles pour s’en...
Article
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AIM: The aim of this study is to evaluate the antimicrobial effect of pre-synthesized novel antibiotic loaded electrospun nanofibers and compare it with conventional triple antibiotic paste when used in patients with immature necrotic teeth. METHODS: Antibiotic loaded nanofibers were fabricated by electrospinning. Thirty-four patients with immature...
Article
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The present study attempted to evaluate the existence and survival of microorganisms in common sea fishes available in Bangladesh and also evaluated the effects of UV radiation (260 nm) to reduce the proliferation of bacterial pathogens. A total of 12 categories of sea fishes were collected from local markets in Dhaka city of Bangladesh. Each sampl...
Article
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The phylum Planctomycetota is constituted by bacteria with unique features that are well adapted to a vast range of habitats. Here, we describe a novel planctomycete isolated from marine sediments collected on a beach in Matosinhos (Portugal) using an iChip-based culturing technique. Strain ICM_H10T forms beige-coloured colonies in modified M14 med...
Article
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Legionella surveillance plays a significant role not only to prevent the risk of infection but also to study the ecology of isolates, their characteristics, and how their prevalence changes in the environment. The difficulty in Legionella isolation, identification, and typing results in a low notification rate; therefore, human infection is still u...
Article
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Skeletal muscle tissue engineering (SMTE) aims at the in vitro generation of 3D skeletal muscle engineered constructs which mimic the native muscle structure and function. Although native skeletal muscle is a highly dynamic tissue, most research approaches still focus on static cell culture methods, while research on stimulation protocols indicates...
Article
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Objective The persistence of pathogenic microorganisms in root canals is the most common reason for the failure of root canal treatment and the necessity of a root filling treatment, which results in an uncertain prognosis due to technical complexity and the variety of highly adaptable microorganisms. This study evaluated the effect of passive ultr...
Article
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Saffron (Crocus sativus L.) is the most expensive spice in the world. This plant species is propagated vegetatively through the formation of daughter corms from the mother one. Unfortunately, many factors hamper the efficient propagation of saffron through this traditional practice. For example, the low production rate of daughter corms and the ris...
Article
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Plum Pox Virus (PPV) is the most important viral diseases affecting fruit stone especially peach tree grown production in KSA. This study was explain to establish large amounts of virus free peach (Prunus persica L. Batsch) plants (cv. Balady) grown in Taif from meristem tips culture utilizing clonal proliferation. In the present study, in vitro er...
Article
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Recent concern for human safety and environmental protection has rekindled interest in natural pigment sources. In comparison to synthetic pigments, microbial pigments show better biodegradability and environmental compatibility and are used in a variety of applications ranging from food to cosmetics. The areas of attention for economical pigment s...
Article
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Microorganisms help in the fertility and endurance of soil. Additionally supporting the growth of several biological systems, soil and soil microorganisms fill in as the best mode for plant growth. Soil microbes are essential for recycling old plant material and decaying organic matter. In the upkeep of the Environment microbial diversity plays...
Book
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This study was designed to determine the factors affecting RB5 decolourization in the environment. A total of 35 isolates (8 bacterial isolates and 27 fungal isolates) have been isolated from wastewater of dyeing and textile industries by enrichment culture technique using 100 mg/L RB5 as a sole carbon source. The obtained results showed that maxim...
Article
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The prevalence and distribution of fungi involved in otomycosis in children of primary school at Taiz city were studied using two types of isolation media. The obtained results revealed the confirmation of fungal otomycosis in 76. 7 % of suspected patients. Ear infection in relation to age the results showed that the group with age between 10- 15 y...
Article
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Replicating the naturalistic biomechanical milieu of cells is a primary requisite to uncover the fundamental life processes. The native milieu is significantly not replicated in the two-dimensional (2D) cell cultures. Alternatively, the current three-dimensional (3D) culture techniques can replicate the properties of extracellular matrix (ECM), tho...
Presentation
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Was soll neu sein an den Debatten rund um die so gut erforschte Lesekompetenz – ist die Betrachtung des Lesens denn nicht obsolet? Weil sich unser Alltag durch die Digitalisierung stark verändert hat und weil diese Änderungen auch und gerade die Art und Weise, wie wir uns lesend informieren, betreffen, steht auch das Konstrukt Lesekompetenz wieder...