Culture - Science topic

I work with iPSC-derived neurons. I got this contamination which is slow growing and spreads across cultures through media. I don't understand if this is bacterial or fungal. My media does have pen strep but still does not get killed. I plated this culture on PDA, no fungal growth. Please provide me suggestions

Dear colleagues,

We are very pleased to invite you to submit your latest research results, developments, and ideas to the 2024 International Conference on Education, Management and Art and Culture (EMAC 2024)will be held in Datong, Shanxi,China on December 20-22, 2024.

The topics of interest for submission include, but are not limited to:

◪Art and Culture

· Art

· Design

· Animation

· Communication Science

· Music and Dance

· Chinese/Foreign Languages and Literature

◪ Education

· Pedagogy

· Psychology

· Science Education

· Special Education

· Physical Education

· Intelligent Education

· Sports Rehabilitation

◪Management

· Safety Management

· Public Administration

· Business Administration

· Safety Science and Engineering

· Agricultural Economic Management

· Information Resource Management

· Management Science and Engineering

Full Paper Submission Date: November 20, 2024

Registration Deadline: November 30, 2024

Final Paper Submission Date: December 10, 2024

Conference Dates: December 20-22,2024

Please send the full paper(word+pdf) to Submission System:

Does anybody know about origin of etymology of numbers in English today? Why do they have those names? I mean, one can find the etymology of number words but I was not able to find initial meaning of those words like five was derived from fist etc. Do you know any sources or books concerning this issue?

The Nazca Lines, located in Peru's Nazca Desert, are a series of large geoglyphs depicting various shapes, animals, and geometric patterns. These lines, created by the Nazca people between 500 BCE and 500 CE, have intrigued researchers due to their scale and the precision required to create them, particularly since they are best viewed from above, despite the Nazca lacking flight technology.

One theory suggests the lines served as an astronomical calendar. Maria Reiche and other researchers have pointed out that certain lines align with celestial bodies, indicating they might have been used to mark important dates or celestial events, aiding in agricultural planning or religious festivals.

Another prominent theory is that the lines had religious or ceremonial significance. Many designs depict animals associated with water, a crucial resource in the arid region, leading to the belief that the geoglyphs were offerings to deities related to water and fertility. This spiritual aspect might have been central to their creation.

Additional theories propose that the lines functioned as pathways or pilgrimage routes. The geoglyphs could have guided people to ceremonial sites or places of worship, serving as part of processional rituals. This aligns with the idea that the Nazca Lines had multiple uses, including guiding pilgrims and marking important communal activities.

While modern research, including satellite imagery and ground-penetrating radar, has provided valuable insights, the complete mystery of the Nazca Lines remains unsolved. They likely had various purposes, from astronomical and agricultural to religious and ceremonial. This combination of theories offers a comprehensive understanding, yet the full scope of their significance continues to be a subject of fascination and ongoing study.

1. Cultural security can be defined as the protection of a nations cultural tradition, against the "threat" of globalisation.

2. National security is the protection of a state's national interests against every external and internal threat.

3. In most cases, each nation is officially represented in the global community by the form of a state.

4. The ancient Greek Herodotus, expressed the opinion that a nation is defined by its common origin, language, religion, and way of life (tradition).

5. That being said, do you think that since a nation's cultural tradition defines the state itself and is closely related to its cohesion, that cultural security can be considered as a component of national security?

"Good music is good music, no matter the genre," says B. B. King, the famous Mississippi-born blues musician (1925-2015); A magnificent quote that beautifully puts into words the sentiment that transcends the boundaries of musical categorization; Regardless of the style or genre. As for Blues, B. B. King specifies "Blues is about embracing your pain and turning it into something beautiful." This poignant quote encapsulates the essence of blues music, shedding light on its transformative power. While pain often feels unbearable, the blues offers solace in embracing these struggles and allowing them to shape something beautiful. By channeling their anguish into music, blues musicians pour their emotions into melodies and heartfelt lyrics. In doing so, they not only release their own pain but also resonate with audiences who find solace in relating to the experiences shared. B.B. King's words remind us of the profound ability of blues music to provide catharsis, healing, and ultimately, the creation of something extraordinary from the depths of pain.

From: https://www.bookey.app/quote-author/bb-king

Hello!

Do you know of any equivalents for culture to Saussure’s distinction between langue and parole for language?

I’m looking for models/theories of culture that are based on a dichotomy similar to that of Saussure (langue/parole), or perhaps to that of Chomsky (competence/performance).

Thank you!

Dear Researchers,

I would like to start working with Caco-2. I have seen in articles that different media (e.g. MEM or DMEM with various suplementation) are used to culture Caco-2. Which is the best (and no so expensive) medium for the Caco-2 cell line?

MEM with L-glutamine and extra + 1% NEAA +10% FBS + 1% Pen-Strep

DMEM (but which?) and extra + 1%NEAA + 10% FBS 1% Pen-Strep

Should I add sodium pyruvate or sodium bicarbonate?

Thanks for your time

Marta

The robots will clearly need to communicate and cooperate not necessarily under human direction.

This suggests a move towards their developing shared beliefs possibly faulty or imperfect.

Which in turn implies society/culture.

That could be interesting, illuminating and perhaps even dangerous in more than one way.

Any thoughts?

In case of Thraustochytrids, I got more than 6 isolates by agar plating. However except Aurantiochytrium spp. all others grow very slowly. What could be the reason behind? I am using GYP broth and agar medium with D-glucose 5g/L, Peptone 2 g/L, Yeast Extract 2 g/L, agar 1.5% in 15ppt seawater medium and incubation temperature is 24-25 degree Celsius. There will be thrasutochytrim and schizochytrium and ulkenia strains. I want to know the reason for the poor growth of the strains other than Aurantiochytrium. All strains are isolated from tropical mangrove waters (leaves, mud, water, algal mats, 10-25 ppt) during December - March months, atmospheric temperature around 35-37 degree Celsius. Can anybody help me?

Does anyone knows the pH acceptable range for virus transport medium (VTM) for Sars cov 2 samples? I supose that it depends if you are only testing by PCR or if you need viability for culture but does anyone has experience in this subject?

Found a studie that defends that in normal individuals with no history of reflux or eustachian tube dysfunction, the pH values range from 6.10 to 7.92 with an average pH of 7.03 (SD, 0.67) so i believe that VTM should be buffered around pH 7 (with a variation of plus or minus 1) but need to confirm that.

Thank you and be safe.

Dear All,

I've been working on differentiating monocytes to dendritic cells (DCs) in vitro using a 24-well TC-treated plate from Corning. Here's my current protocol: I place 2 Million PBMCs in each well, and after one day, when monocytes should be adherent, I carefully remove the supernatant and add RPMI with IL-4 and GM-CSF. After seven days, when I inspect the cells under a microscope, I observe that the Dendritics are not fully formed, and the cells appear smaller than expected. Additionally, they are not fully adherent; if I attempt to wash them, most of them detach. I also notice the presence of other immune cells, possibly alpha-beta T cells, which are similar in size.

I've attempted monocyte purification, but previous method seems to result in more dendritic cells.

Any suggestions, experiences, or recommendations on how I can optimize the purification and differentiation of my DCs would be greatly appreciated.

Thank you in advance.

How do we define heritage, and who decides its parameters? Scholars have pointed out that heritage is a dynamic process and not a mere monumental object.

Part of this "anti-monumentalist" approach is our appreciation of heritage. Hence, the question is: Can heritage be found in non-heritage contexts?

What if we look at our socio-economic dynamics? Let's say, we take a look at the spontaneous movement of people through an outdoor market, or a public bus moving through the metropolis, or a street football pitch?

Is heritage only associated with a specific timeline? Is it culturally justified?

Warning somewhat of a pun: Being self-owned is often both literal and figurative because the autonomous often self deprecate.

Hi! Who can direct me by the culture medium the most advantageous for the isolation of fungi from the soil ( sabouraud or PDA)? 

My formula is often humorously both observational apathy and not serious inaction, executed as ,somewhat self therapy, with jokes(which are minimum words before punchlines, delivered and timed well enough for the audience to laugh). Sometimes audiences pick comedians based on how much they relate.

Who agrees platitudes from the intellectually disabled should receive more tolerance? How? Why?

Dear Researchers and Scientists, Friends and Colleagues,

In my opinion, answering the above question, I state that the role of applying the principles of ethics, culture and respect towards other people, other cultures, other value systems, etc. in the development of scientific cooperation, including the conduct of interdisciplinary research projects, scientific cooperation within the framework of international teams of researchers and scientists, within the framework of the ongoing discussions on the discussion forum of this Research Gate portal. Do you agree with me?

In the discussion forum of this Research Gate portal, in order to inspire discussion on various research topics, I have formulated many questions and theses.

I am glad that many of these questions turned out to inspire discussions involving many researchers, scientists, experts in a particular field, people who conduct scientific research on a particular issue, people who publish in their scientific publications the results of their research, people who are interested in a particular issue taken up in the mentioned questions posted on the discussion forum of this Research Gate portal.

I am glad that the questions posed on this discussion forum of the Research Gate portal have proved to be an inspiration for interesting discussions, in which noble researchers and scientists participate. In the course of these discussions, which sometimes take on the character of brainstorming, exchange of ideas, new inspirations for further research topics emerge, new research concepts are formed, proposals for new topics for theses are made, etc. Within the framework of the discussions held, researchers and scientists exchange their experiences of their scientific research, present their research results, inspire scientific cooperation, point out other new insights on certain issues. Thanks to this, there are inspirations for new research directions, for undertaking scientific cooperation in various topics and also for interdisciplinary research teams, etc. This is the key purpose of this discussion forum of the Research Gate portal.

However, I am very saddened when the participating researchers and scientists in the framework of the discussions held, instead of a desire for understanding, understanding, respect towards other people, the participants in the discussions representing other scientific communities, other cultures, other countries instead of respect and culture generate negative emotions, instead of substantive arguments in support of their theses turn to other Persons with invectives and negative expressions, i.e. act contrary to the principles of ethics and the basics of culture, ignoring the principles of ethics of research and science, also ignoring the principles of ethics of this discussion forum. So I very much ask you to follow the rules of culture and respect other people participating in discussions. This is important because aggression generates aggression and respect generates respect. Hopefully, looking to the future, I believe that those fortunately mentioned above situations of negative nature were few and in the future there will be none, and that on the discussion forum of the Research Gate portal, everyone participating in discussions will apply respect to other people, participants in discussions and also regardless of whether other participants in discussions represent other scientific communities, other cultures, other countries and so on.

Faced with the development of various global problems, in trying to solve these problems we should all look for what unites us and not what divides us. This is the key to the development of our scientific cooperation and also to jointly solve various global problems.

Therefore, in the situation of participating in discussions on this Research Gate portal, I therefore very much ask you to apply the principles of ethics, culture and respect for others.

And what is your opinion on this topic?

Please feel free to respond,

I invite everyone to join the discussion,

Thank you very much,

Thank you,

I extend my warmest greetings to all participants in the discussions on the Research Gate portal,

Dariusz Prokopowicz

The campus/college environment plays a pivotal role in the learning of students and faculty members. In your opinion, what are they, and how to ensure them?

Hello! My SY5Y cells shapes were normal when firstly received(Figure 1), but changed into something like amoeba after digesting with pancreatin/EDTA for merely 30s(Figure 2, red arrow). The cells still multiply after shapes changed, but seems to be more easily to form into clumps and float. It makes me hard to perform experiment like phase contrast shooting. Does anyone know the reason? Or ever encoutered such case once? Appreciate for any suggestion.

Hello,

I'm working on plant tissue culture. Sometimes the tissue I cultivate produces what you can see in the photos attached.

I'm wondering if these are also calluses, or they can be organ regeneration?

Thanks in advance for your precious comments

Hello all,

We cultured MSCs on calcium phosphate discs for 3 days and 7 days. We are seeing strange crystal-like precipitates or something of the sort (images attached). They are found wherever cells are found, or nearby cells, that are growing on the surface of the discs. We did EDS on these samples out of curiosity and the crystals appear to have a high concentration of NaCl, which indicates that they are salts.

I can't find any literature that shows this happening in their cell studies. Has anyone else seen this sort of thing happen in their cell cultures? I have no idea what could explain these results and I would appreciate some insights, or hypotheses, if any.

Thanks!

Storage and maintenance of pathogens is a costly and time-consuming affair, the recent study indicated that most of the pathogenic bacteria can be stored for several months at room temperature in sterile tap water without any hustle.

Ref DOI: 10.13140/RG.2.2.34672.84480

I need to do a primary culture of Non parenquimatic liver cells from mice and althought, I have the protocol for the obtaining and isolation of these cells, I do not know which medium to use, what porcentage of FBS use and what and how much supplements use (like Glutamine, antibiotics, etc).

I would really appreciate the help!

‘Entrance to courses is frequently restricted by high prerequisites in terms of prior academic performance (Arendt, Lange, & Wakefield, 1986; Crawford-Lange, 1985; Lange, 1987). This elitism is curious when one considers that it operates under the assumption that some students cannot learn a second language when virtually all students have achieved proficiency in a first language (Crawford & McLaren 2004, p. 141).

Should Higher Education institutes in native English-speaking countries request from Non-native English Speakers (NNES) English proficiency requirements for entry without mandating the same proficiency tests for Native English Speakers (NES)?

Some Higher Education institutes in native English-speaking countries require proof of proficiency from Non-native English-speaking individuals for entrance. There is no question that students need to communicate in the target culture language. However, these institutes enforce strict IELTS band scores for each language skill (reading, writing, speaking, and listening) from NNES but do not mandate that NES undertake the proficiency test. This assumes that NES are naturally skilled in reading, writing, speaking, and listening, whereas, in reality, not all NES have strong writing or reading skills.

Arguments to consider:

1) Some NNES might have exam anxiety, which puts them at a disadvantage when taking English proficiency tests.

2) Some topics in English proficiency tests are specific to NES cultures that NNES may be unfamiliar with.

3) NNES should have the opportunity to be accepted regardless of their English proficiency scores with options for prerequisite courses for improvement.

4) Different cultures have different writing styles. Language Tests assessors might not be familiar with these cultural differences, which may affect grading.

I would like to have some recent (since 2018) academic sources that illustrate the theory of Aesthetics and how different societies have different perceptions of it. I am looking for Eastern and Western thoughts on it.

The unique adaptive strategy and cultural space of diverse tribal communities has gained huge research attention across the world owing to complexities of the issues related to their bio-cultural diversities, ethnicity, historicity, response to state policies and ongoing negotiation processes in a globalise world. Depending on their exposure to the forces of change and intricate link to the wider socio-political realities of life these issues have accelerated the process of transition/transformation among the diverse tribal population. In view these facts and circumstances, what should be role of human science and other related disciplines to deal with the emergent contemporary issues, which have local, regional as well as global implications?

I (Japan) and colleagues from the Philippines and Vietnam are conducting a survey on the English language and culture.

We would like to open this to any other interested parties.

We are all teaching EFL students who are English majors.

It is a simple survey that questions how long students have been studying English and how culture effects their foreign language learning.

We would like to expand this to a wider base of countries, not necessarily Asia with instructors who are teaching EFL English majors.

Please contact charry@g.matsuyama-u.ac.jp

Thank you, for any who are considering

Harry Carley, Matsuyama University, Ehime, Japan

Half a century ago R.J.Havighurst published a book on developmental tasks. Are they still valid? What do we have to learn as humans during certain stages of development? Do the stages differ in lasting now? Which are shorter, which are longer? What we do not have to learn any more? Is there something new we should learn?

Hi,

I'm wondering if anyone has experienced this.

I'm working with a species of actinomycetes, and after a certain time the media colour changes to an almost red/maroon?

Is this melanin production? I'm unsure as to what it could be?

Thank you in advanced

Dear scholars,

I have a feeling that the discussion of traditional performing arts within Cultural Evolution is almost non-existent. Maybe because the nature of traditional dance is too complex? It seems that performing arts research falls mainly either within cultural and anthropology but never within Cultural Evolution. Is it because it is impossible to discuss? What are your thoughts?

It is the trending topic for a publisher this week.

I want to learn the opinion of Academia members. Is it due to environmental factors or personal over-ambition?

Why does anyone commit fraud?

Hi!

Bit of a strange question.

This Amycolatopsis culture has been growing for 7 days, it usually grows white colonies. I have added 100ug/mL hygromycin to check for natural resistance and the culture is turning yellow?

Why would this happen?

Thanks!

Hi!

I left an amycolatopsis strain to grow in double autoclaved SFM agar for three days and the agar has turned black?

Anyone know why this might be?

Thanks!

The publication of Abraham Moles' "Sociodynamique de la Culture" in 1967 sparked new reflections on the relationship between the person, society and the modes of mass communication. Moles highlighted the intense relationship between the individual, his culture and the dynamics that develop around it. His approach to sociodynamic theory applied to message transfer reminded us about the dynamic and social processes that interfere with how a message is sent, interpreted, registered and shared. Although his work focused on radio and television, we could apply the same principles (with some breadth) to digital media. Nevertheless, how could we speak of a new Sociodynamique de la Culture in the current context? What new reflections can we draw from Moles's sociodynamic theory?

Has anyone faced contamination from media containing Yeast Extract and Tryptone while working with SDS-Gel of proteins from a culture supernatant? If yes, what were the molecular weight of the bands and any idea about the nature of the protein showing on gels?

Family Systems Theory by Murray Bowen is a specific approach which takes familial functioning into account. I am interested in how the concepts present in it will translate into a South Asian (or, to be more precise, the collectivistic framework of Pakistan) context? For instance, Bowen's theory is based on an understanding of nuclear family systems but how would it apply in the case of a joint family? In a culture where parents might be dependent on the views of the community in terms of bringing up children, how would Bowen's theories translate exactly (for instance, if they have a disabled child)? For example, what is the difference between Bowen's concepts of relationships between generations and the kinds that might emerge owing to different generations living under the same roof and with their extended family members as well?

Let's say, for instance, that it is not merely parents but also other members of the family such as extended family members or grandparents who either counsel children on "appropriate conduct" or even express disapproval and view it as appropriate behavior culturally. How do Murray's concepts such as "Differentiation" change in a cultural sense in that case just as one example out of many possible ones? In a culture where "adulthood" and transition towards it might exist in a legal sense but might not be necessarily viewed as "important" even for parental figures (for instance, even if children cross the age of 18, parents do not try to treat their children as "adults"), how would Bowen's concepts change? I am not talking in terms of applying these concepts therapeutically, but, in terms of how they might be applicable in a conceptual sense.

Dear fellow researchers,

I am planning to conduct a research related to Ethnic Diversity composition in schools (e.g. ethnic diversity composition per classroom). Could you please share with me a questionnaire or instrument or the formula for measuring Ethnic Diversity Composition? So I can categorize the Ethnic Diversity Composition into high Ethnic Diversity and low Ethnic Diversity (e.g. 1 for high and 0 for low).

Best,

Edita

As known, the whole world is moving towards a renaissance in education which required transferring the expertise between countries. In general, some of the developing countries use the transferred system as it is without keeping into consideration the difference between cultures.

How is it bad to transfer the educational system that followed by a certain culture to a different one?

Could this affect the desired goals in the development wheel negatively?

A quest for real-world examples (Best Practices) from developing countries.

Some research findings show that there is a significant relationship observed between entrepreneurship education and entrepreneurial culture. Given the private sector’s involvement is essential in economic development and also that the capacity to recruit new graduates in the formal sector is very much limited, it’s highly vital that existing and new entrepreneurial cultures are adequately exploited. And, it is also necessary to link this cultural dimension with formal entrepreneurial education.

Is there any program/project successfully implemented so that those two issues are properly linked and resulted in achieving the desired goal (As Best Practice)? (Good if country and program/project examples are mentioned.)

Many thanks!

I am going on fieldwork over the summer to collect plant tissue for eDNA analysis of endophytes. I would also like to try and culture endophytes from some of these plants. Is it possible to store leaf segements in buffer to import them back to my lab to start the culturing process?

What buffer would be suitable for this and how long could the leaf be stored in the buffer?

Any papers with similar methodologies appreciated.

Hi everyone! We have a bit of a problem in our lab where every now and again we get a very strange contamination in TC (please see attached photos/ videos). Our work is completely pen/strep free with no added antibiotics, but this contamination looks like no bacteria I have seen before. The movement is also very strange and sometimes they can be seen almost cartwheeling/ doing 360 degree spins in the flask. I would just love to know if anyone has experienced this before and what I can do to make sure it doesn't happen again. Thanks so much in advance!

I have asked a question, If baboons appear more like us than any other of our primate relatives, is it sane if I think in reconstructing biological taxonomy on a Cultural basis? Now, I want to ask about the meaning which Culture is supposed to be if this taxonomy wants to go further.

More and more museums present their collections of works of art in the form of reproductions published on the Internet. The development of Internet information technologies and the Internet of Things contributes to the popularization of art and culture in society.

Do you agree with me on the above matter?

Please reply

I invite you to the discussion

Thank you very much

Best wishes

Bone marrow is collected from the femur and tibia of C57BL/6 with the aid of 1X PBS ambient temperature in a 50mL falcon tube. Centrifugation is performed at 1500 rpm, 4°C, 5'. The culture is composed of 50% RPMI, 30% LCCM and 20% fetal bovine serum for 7 days, and on day 4 the medium is supplemented in the plates following the same amounts. However, on day 7 there is no differentiation of BMDM, there are no cells adhered to the petri dish. Could you help me?

I used second hand data so I don't have the manual. I tried to find it for a long time.  If you have the copy of the scoring manual, could you please send me in order to use correctly my results?

I've seen evidence of strange behavior among people who walk their dogs on trails in parks or seminatural areas in my suburb. People will carry plastic bags for picking up their dogs' feces but then, instead of discarding the filled bag in a bin, they will throw the bag away like litter, and often in the vicinity of the bins. (I've never actually seen anyone do this, just the evidence thereof.) This behavior creates a more serious pollution issue than not cleaning up after your dog since the plastic bags don't biograde. Have there been studies on this or similar counterproductive behaviors?

This is a liquid culture of skin scrapping in Ampicillin containing media.

Is it sensible to spend time and effort learning Latin today? Why? Why not? Which specialists really need it? Should this language again be in the curriculum of all (or, at least, most) schools?

I Am trying to culture lactobacillus and Candida together. I am having difficulty because lactobacillus likes to grow anaerobically whereas candida prefers aerobic conditions. If anyone has any suggestions on how to solve this dilemma, growing them together, can you please let me know? I am using MRS and YPD media 50%:50%

Our lab is conducting a research project about Asian-American families. We are investigating Asian-American families' well-being during the COVID-19 pandemic, specifically, their experience of racial discrimination, their parent-child relationships, and their children's development. So far we manage to contact churches, organizations, and school communities, but we still need more Philippine and Korean teenagers (12-18 years old) and parents to participate. Any suggestions to help us recruit? I appreciate your help.

Hello,

Has anyone already changed medium composition between "before" and "after" freezing? i.e. medium without FBS before freezing , then freezing (in presence of DMSO), then thawing in another medium with FBS? What would be your thoughts or possible experience already?

Thanks,

Roland

Hello everyone,

I am looking for links of audio datasets of indigenous Mexican languages that can be used in classification tasks in machine learning.

Thank you for your attention and valuable support.

Regards,

Cecilia-Irene Loeza-Mejía

Himalaya is situated in varying rocks, soils, altitude, slope, aspect and socio-cultural practices which is unique than other environs so which soil erosion model is best for Himalaya?

I have a strange problem.

I prepared 500 ml of nutrient agar and 500 ml of Sabouraud.

After autoclaving, I pour them in petri dishes but many plates still semi liquid .. !!

I repeate the process for several times and the problem still occured.

Why..?

and how I solve it?

Is the problem may be ..

Manual mixing?

Programming of the autoclave?

Any source of vibration before solidification?

or other reasons?

Also, I used 3 ml of agar agar to solve this problem but it doesn't work.

Hi everyone,

I grew Staphylococcus Aureus bacteria on BHI/TSA plates from a frozen stock.

Then, colonies were picked and inoculated in liquid media - either BHI/TSB.

In both cases, I see significant sediments in the liquid. Is that normal? Are these dead cells? How should one properly measure the O.D in such a case?

Thanks!

What is the best culture medium and growth condition (pH and temperature) for production of industrial bacteriocin by lactic acid bacteria including Enterococcus faecium, Lactobacillus salivarius, Lactobacillus plantarum, and Pediococcus acidilactici?

Proverbs are the pride of many nations around the globe. They offer one with teachings of wisdom in just a sentence. Proverbs have a deeper meaning that give them their sweetness. Some may sound provoking, but no contesting that they define the society belief systems as well as principles.

We are analyzing the behavior of groups of Stylonychia mytilus and have already some interesting preliminary results. Unfortunately our strain died and we are not able to find a new supplier. Is there a culture collection - possibly i Europe - that can provide the species or does someone work with the organism and can help us?

Hi! I am culturing CD8+T cells from the spleen of infected mice, but they die a lot after ~16 hours.

Any recommendations (such as cytokines) to increase their survival?

IL-2 is not increasing it.

Thanks in advance

I have been trying to start some cultures of thermophilic cyanobacteria (in the temperature range of 60-68°C), from environmental samples and from stock cultures, both of which have been stored in a walk-in fridge for 3-4 years. The samples and stock cultures are green in the fridge, but when I try to set them up in the incubator (in 75mL of BG11 medium in flasks, at source pH, under continuous illumination), they photobleach within 3 days. At first, I just set the inoculated flasks into the incubator and raised the temperature with the flasks in it. Then, I've tried several methods to keep the transition more gradual: (1) keeping the inoculated flasks in room temperature in the dark for 1-2 hours prior to moving them to the illuminated incubator and raising the temperature of the incubator while they're in it, (2) covering the flasks, putting them in the incubator, and raising the temperature 10 degrees every hour until they're at the goal temperature, (3) keeping the inoculated flasks in room temperature in the dark for 1 hour, then moving them to the incubator while covered so that they remain in the dark, then raising the temperature 10 degrees every hour, and uncovering them when the temperature reaches 50 degrees.

Could anyone offer me any advice on how to successfully get these cultures growing? Thanks in advance!

Empirical studies found that fear (e.g., fear of happiness) is detrimental to one's psychological well-being. While the result is consistent with our common beliefs, I wonder what theory can be used to explain the negative relationship.

> Animals are kept in accordance with ABNT standards.

Hello everyone!

We’ve started to work with this cell line, and it is driving us crazy. We are unable to making them attach to the plate and when we do, we see a huge amount of apoptosis and cell death.

The coating is performed with PDL (50 ug/mL) and laminin within a range of 6 to 12 ug/mL. Those parameters are what it’s written in most of the limited bibliography that exist about this cell line, so we are unable to find what’s the problem.

Has anyone experienced the same problem as us? Did you manage to solve it somehow?

The pics are showing how our cultures looks like in bright field microscope from 2 – 5 days after passage approx.

Thank you in advance!

Kind regards

I need a tool to explore cultural identity and hoping there is a widely used tool or scale for different cultures.

Knowledge is what distinguishes human species from others, but how we use this knowledge depends only on man. Man can use his intelligence and knowledge wisely, ie according to the innate need for self-preservation instinct and survival of the human species in changing environmental conditions, including the ongoing global warming process. But he also may not use his knowledge and intelligence for this purpose.

However, one should think positively that the previous generations, previous cultures, previous ancestors in the process of evolution and the process of development of cultures, development of civilization and finally development of technology have created modern man as being equipped with intelligence, self-awareness, knowledge, technology, i.e. unique instruments, thanks which man became a dominant species on Earth. But will it always be the dominant species on Earth? This question remains open. It will be settled in the future.

Knowledge, huge resources of knowledge and various possibilities of its use is what only one species has on Earth. Knowledge gives people a lot of potential to complete the next stages of technological and civilization development. However, if this knowledge allows to forecast new categories of natural, climatic and civilizational phenomena and to secure adequately against appearing threats, to use it as a development opportunity, time will show.

The following question may be added to these considerations: Will dinosaurs survive the global cataclysm caused by the impact of a large meteor on Earth, whether they survive global warming, drought, limited food resources and areas suitable for existence, if they knew when, where and in what form this cataclysm would occur? ? If dinosaurs survive this or still on Earth until now they would dominate as known dinosaurs or would a new species of intelligent post-dinosaurs be created? These questions will remain open due to incomplete knowledge in this topic.

If the knowledge in this matter would be supplemented in the future, will it change anything about the possibility of adapting people to such cataclysms that have destroyed dinosaurs? Not necessarily. Dinosaurs could only know what they could know, having a much more limited awareness of natural and cosmic phenomena. Dinosaurs could know very little about what a person knows, but they did not have the awareness of lack of knowledge and the need to increase knowledge resources through the development of scientific research. Man is probably the first species who, thanks to his intelligence and built knowledge, also has an awareness of the potential lack of knowledge. Man has this knowledge, is equipped with specific instruments for conducting scientific research and the possibilities of expanding knowledge resources. Man already possesses this knowledge, but will this knowledge prove to be helpful in surviving future cataclysms, their outpacing and securing them against them? The answer to this question will appear in the future and it is not yet decided. So the development of knowledge and what is being discovered, created with the help of knowledge, intelligence and creativity, should also be carried out wisely. Wisely, ie according to the need for self-preservation instinct, with the need to survive the human species.

This need should take into account the necessity to implement sustainable ecological development in the development of civilization, protect the natural environment, care for biodiversity in the natural biological ecosystems, individual species of flora and fauna, genomic composition in gene banks of endangered species of flora and fauna, that is, preservation of knowledge about biodiversity , natural resources of weeds created by nature. This we know thanks to our intelligence, self-awareness and civilization-generated knowledge. But we also know something more. We know that humans are probably the first species that, thanks to their intelligence and built knowledge, also has the awareness of the potential lack of specific categories of knowledge.

There are many questions that we do not know the answer to. It is also part of your knowledge or these questions on the government are unanswered from this knowledge. These questions would not exist if there was no intelligence, knowledge and built culture, civilization, technology, etc. Man has this knowledge, is equipped with specific instruments for conducting scientific research and the possibilities of expanding knowledge resources. Which questions will we get in the future thanks to our scientific research, will these be satisfactory answers for us? One thing is certain, the resources of knowledge will increase. But how we use this knowledge depends only on us.

Do you agree with me on the above matter?

In the context of the above issues, I am asking you the following question:

Please reply

I invite you to the discussion

Thank you very much

Best wishes

Religions are an important part of the cultural richness of the society and they should be respected. However, those working in science may face questions about it, as the tenets of some religions have been claimed to be against modern scientific evidence.

How do you feel about it? Do you see inconsistencies? Do they cause you unrest? Or do you have a way to reconcile science and your religion?

Thank you for sharing