Science topics: CucurbitaceaeCucurbita
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Cucurbita - Science topic

A plant genus of the family CUCURBITACEAE, order Violales, subclass Dilleniidae, which includes pumpkin, gourd and squash.
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I'm doing a research on winter squash, but i have a hard time finding any scientific data about the suberizationprocess in winter squash.
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The suberization process in winter squash is influenced by several factors:
  1. Temperature: Warmer post-harvest temperatures (25-30°C) promote faster suberization, while lower temperatures slow it down.
  2. Humidity: High humidity (90-95%) is needed to prevent tissue dehydration and support suberization.
  3. Wounding: Suberization is triggered by wounds during harvesting or handling, as the plant forms suberin to protect the tissue.
  4. Oxygen Availability: Adequate oxygen is essential for suberin biosynthesis, while anaerobic conditions can hinder the process.
  5. Hormones: Hormones like abscisic acid (ABA) and ethylene regulate suberization, increasing in response to stress.
  6. Calcium: Calcium helps with cell wall integrity, supporting the suberization process.
  7. Harvest Timing: More mature fruits suberize better than immature ones.
  8. Cultivar Differences: Genetic variation among squash varieties affects their ability to suberize.
In short, suberization is affected by environmental conditions, physiological factors, and the specific cultivar of winter squash.
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I'm doing a study on the shelf life and suberizationprocess of winter squash. I couldn't find a study that specifically researched the factors that influence the shelf life of winter squash.
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Thank you all
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REGIONAL AND SOCIAL DIALECTS
This PowerPoint discusses phonological, morphological, syntactic, and semantic differences in New England, the South, the South West, California (Valley-Girl, and Surfer Dude), the Borsht Belt (Yiddish influences), Down-East Yankee, Minnesota (Lake Wobegon), Brooklyn, Western, Canadian, and Star Wars (Yoda) regional and social dialects.
The frontier humor of the American West or of Australia tends to be exaggerated: He is so stingy that he sits in the shade of the hackberry tree to save the shade of the porch. His feet are so big that he has to put his pants on over his head. His teeth stick out so far that he can eat a pumpkin through a rail fence. When Slue-Foot Sue married Pecos Bill, Sue insisted on riding his horse, Widow-Maker. Widow-Maker bucked her off and she bounced so high on her spring bustle that she orbited the moon and they had to throw jerky to her to keep her from starving to death. When Pecos Bill died, they marked his grave site with, “Here lies Pecos Bill. He always lied and always will. He once lied loud. He now lies still.”
Joe Barnes was sired by a yoke of cattle, suckled by a she-bear and had three sets of teeth and gums for another set. Nimrod Wildfire was a touch of the airthquake. He had the prettiest sister, the fattest horse, and the ugliest dog in the district. Wirt Staples has a shadow that can wilt grass, breath that can poison mosquitoes, and a yell that can break windows. Mike Fink was a Salt River roarer, a ring-tailed squealer, half wild horse and half cock-eyed alligator and the rest crooked snags and red-hot snappin’ turtle.
Don and Alleen Nilsen “Humor Across the Academic Disciplines” PowerPoints:
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What is the correlation between regional dialects and social dialects? Any comments you make about this PowerPoint will be appreciated.
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Symptoms observed on Zucchini plant..
Only experts needs to react please..thanks
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Rupinder Singh Dear Rupinder Singh, I am sure that you can isolate from these spots some pathogenic Pseudomonas and Pantoea bacteria, but it is hard to say about true reason for this disease. Bacterial plant disease diagnostics by photograph only is not reliable. You have to provide additional information - time/plant growth stage of the first symptoms occurrence, dynamics of spots development, weather conditions at least for 2 weeks before the disease occurrence (temperature, rains, air humidity), chemical treatments inlcuding fertilization, irrigation type, number of affected plants in field, location of the diseased plants in the field (field margins, spots, spreading), previous crop on this field, current crops around the zucchini field.
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Biointegrity is about ensemble ornamentation radiate energy do to weathers forecasting elements, combination of the energy and warmth of the sun with the cozy closeness of the winter to what the future hold in the spring. Seed, grains and possible derivative of the green-faced which in modern harvest of pumpkin, turnips and rosemary. As security exchange, money focused on feeling of prosperity and security. Dough is smooth and elastic in consistency but the scope and scale is a way to gauge accuracy as progress. Harvesting is a timeless energy of a wonderful feeling of community. Winter origin in distant past, ice age, originally still made of turnips, apple bobbing, ghostly world, even-trick-or-treating have spring significance which focus on let go of the past and has connected to the future of autumn and pumpkin. This is differentiation. In term of audio quality, internet radio is typically at the low end of the fidelity spectrum which improve with an aural optimization mostly reliable on making the choice just like all different formats of terrestrial radio stations simulcast over the internet with excellent sound quality. A service is social in nature in sharing favorite with social networks from a lot of selections, collections and variety. It worth checking out that enough newer programming makes a viable options a nice perk for all prime. Think about basic cable or broadcast television offering a lot current and classic programming plus the vintage stuff some specialize in newer programming and some in older stuff are certainly an appealing proposition connect to network system of the fidelity spectrum on infinity system.
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BIS for MIS and Endowment funds
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For the pumpkin is paramount starship, this first step is Corporate finance
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Bat feeding on the Guava.
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I have been reviewing various journals for the visual leaf as well as fruit damage scoring of Red Pumpkin Beetle (Aulacophora foveicollis). It would be really helpful if any of you have some authentic source or reference for this evaluation.
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Dear Kalyani, something similar to the method used in this work?
Rashid et al 2016, Population and infestation assessment of red pumpkin beetle (Aulacophora foveicollis Lucas) and management using botanicals
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Powdery mildew on squash leaves caused by (Sphaerotheca fuliginea or Podosphaera xanthii?
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Podosphaera xanthiiis the proper name. Sphaerotheca fuliginea is synonym for the same fungus.
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I'm experiencing damage to my pumpkin vines, particularly on new growth. Chunks parallel to the vine are removed, flowers are missing, and some new growth appears to be gnawed completely through. I have yet to catch the culprit in the act. Any ideas?
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gusano barrenador de tallo (Mellitia ciucurbita)
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I am looking into developing a chemical profile of Spinach, Kale, Pumpkins and Beetroot (and similar vegetables) which could be further used in authentication of purees of these vegetables. The analytical question would be - how do I distinguish that a spinach puree is made of 100% spinach, and not of 30-50% another (cheaper/more available) leafy plant?
Given that a profile of semi-finished goods (juices/puree) of fruits are well studied, and we have several authenticity models - what parameters could be included in the authenticity analyses of such vegetable products? Drastical technological difference between fruit and vegetable processing should be taken into consideration as well.
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Dear
Rani P Ramachandran
, thank you for your input. Again I would like to mention that fruit purees are nowadays a more or less explored field in regards to authenticity, whilst vegetable purees are not. The shared article belongs in the pools of fruit products.
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Hello, I am trying to use the root squash method to observe mitosis in almond (Prunus) shoot apical meristems, but I'm not getting clear staining of chromosomes. This is the protocol I'm using so far:
1) Collect apical buds from the tree and preserve in a cooler on ice.
2) Dissect an almond meristem and inner-most leaf primordia from an actively growing apical bud.
3) place meristem on a microscope slide. Treat with an even mixture of HCl (I've tried 2M to 0.5M) and aceto orcein dye (I've tried 1% to 0.025%).
4) Place slide on a hot plate on low heat to evaporate the mixture. Don't dry completely.
5) Resaturate meristem with aceto-orcein dye and place a coverslip on top.
6) Cover slide with a paper towel and "squash" the meristem into a single cell layer by pressing down with an Erlenmeyer flask.
7) view slide under microscope starting at 4X and going to 1000X (oil immersion)
I'm very confident in my ability to excise meristems. I believe the HCl is properly dissolving the pectin from between the cell walls and "squash" step are correct since the meristem appears to be in a single layer of cells when viewed under the microscope.
There doesn't seem to be anything in the literature about shoot apical meristem squashes. Is there a good reason for that? Does anyone have a suggestion to improve the result? the first 2 photos are the shoot apical meristems (using different percentages of dye). The second photo is from a colleague who applied the same method to rice roots and clearly shows the chromosomes.
There doesn't seem to be anything in the literature about shoot apical meristem squashes. Is there a good reason for that? Does anyone have a suggestion to improve the result? Thanks in advance!
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Try to collect samples at the morning and fix it in carnoy's solution.
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The plants were healthy at vegetative stage but at flowering stage in some plant leaf structure changed and gradually this symptom spread in many plants in field. We uprooted more than 20 plants and still this disease in field has potential to reduce yield dramatically.
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Yes, it is a viral disease of Cucurbita pepo caused by Zucchini yellow mosaic virus (ZYMV), which belongs to the genus potyvirus and is one of the most destructive and widespread viral pathogens on Cucurbits worldwide.
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I need method to obtain aquous extract of pumpkin seed in solid form powder at end
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Obtain a concentrated aqueous extract of the pumpkin seed. Use freeze drying to dehydrate and conserve. Container might be filled with nirgen gas to add the longevity.
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How much residues/biomas and bioenergy (TJ) can be derived from 1 ton of each of the following crops:
Millet ,
Sorghum ,
Maize ,
Rice, and
Groundnuts
How much residues/biomas and bioenergy (TJ) can be derived from 1 ton of each of the following crops,
Mangoes,
Orange,
Grape fruits
Avocado
Banana
Lemon
Coconut
Pawpaw
Cashew
How much residues/biomas and bioenergy (TJ) can be derived from 1 ton of each of the following crops,
Tomatoes
Bitter tomatoes
Egg plant
Cabbage
Onions shallots (leaf)
Onion bulb
Cucumber
Okra
Sorrel
Lettuce
Carrots
Pumpkin
Water melon
Big pepper
Small pepper
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Details of the higher heating values, crop to residue ratio, feed rates and conversion efficiencies of the selected technology are crucial to determining the bioenergy potentials of the crops. This paper may be of help to you;
Mendu, V., Shearin, T., Campbell, J.E., Stork, J., Jae, J., Crocker, M., Huber, G. and DeBolt, S., 2012. Global bioenergy potential from high-lignin agricultural residue. Proceedings of the National Academy of Sciences, 109(10), pp.4014-4019.
Hope this helps...Regards, BBN.
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I am conducting an experimental research on the germination enhancement of squash affected by mechanical, chemical and thermal scarification methods..i have researched about time- to-event method in collecting data but I am confused how will I do it??is this method appropriate for my study??
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Dear Shernie,
yes, Andrew is right. However, if you have more time lapse data, then you can compare waveforms of functions.
You will plot the "collection times" on the x-axis and the values of the "measured characteristics" on the y-axis. Than you can compare the curves of the control sample and the treated samples in one graph.
The number of repetitions is also important. I usually use a pattern where I have 30 seeds x 5, ie 150 seeds per treatment (or control).
Let the experiments succeed.
Regards, BS
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Following an experiment where Zea mays and Cucurbita pepo seeds were planted individually and together, results showed a positive allelopathic effect on germination and seeling lenght as both seeds had 100% germination when planted together as against 92 and 96% when planted individually. The seedling length was also positively influenced by this interaction.
My question now is:
Why do the allelopathic produce this positive effect?
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Thank you all, I'm grateful for your contributions, it helped broaden my knowledge.
I actually found the under listed and felt it really answered my question of Why do the allelopathic produce this positive effect?
In the experiment, the following are possible:
1. Their exudates may act as an antifungal that controls attack on seeds.
2. The exudates may act as seed priming agents.
3. Positivity in seedlings length be may be a result of early germination advantages or early competition.
4. Their exudates may also be a source of plant nutrients for the seedlings.
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I prepared flower buds to karyotype for Yam crop. Flower buds were collected at 10am and directly fixed 3:1; ethanol and acetic acid for 24 hrs. and kept at room temperature. Pollens were removed from anther, squashed and stained with FLP ORCEIN solution for 60 to 90 minutes to allow enough dye accumulation. The prepared samples, mounted between slides and coverslip, were observed under 40 X magnification and captured at 100X magnification with oil immersion using an optical microscope. Another steps involves squashing of flower buds without removing anther/pollen because of using small buds size at tip location. I am only observing mitotic cell division. Please, download the chromosome pictures here that someone can guide me further:
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Hello Andrew, thank you very much. The somatic cell of this specie (Diocorea rotundata) is 2n=2X=40. Does that mean I should look for chromosome of 20 in the cell metaphase?
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I've been taking to a young geneticist from New Zealand, at the beach in Formentera. She agreed with me, analyzing and comparing genetic codes for Yemeni 'pricky pears' tree and Mexican one, we should know from where proceed the plant, that way solving some historical mysteries:
- Were Aztecs and more extensively, all American Indians, too stupid to squash an insect (cochineal) between fingers to get bright red color ?
- How comes in the fresco representing Mexico city, the eagle looks more Roman (very similar to a one found in Merida, Spain) than Quetzacoalt? How comes it does not look like graphically the Aztecs bird representations?
- How comes the Spanish Royal color was of Arab origin? By Charles Quint's times, Spain wanted to be dissociated from its Arab past, so they wished a cochineal of their own offering a red color they could call truly Spanish.
- How comes there are no prickly pears in Amazonian forests, nowadays dominated by plants grown by pre-colombian?
- How comes in America, it does not grow on the sea level when it expanded so well in Mediterraneo at sea level it was used by Muslim sailors as an amer to know if you've already been in the place?
- How comes Napoleon III, 19th c. dreaming of an imperial army in red trousers (without being aware he transformed his soldiers in easy targets) and wishing to provoke in Mexico a commercial earthquake, refurbishing the old 'traditional' and 'historical' cochineal production on prickly pears tree, he had to both reintroduce cochineal and prickly pears tree in Mexico?
-How comes in Spanish 'prickly pears is called "higo de Barbarie" (North African fig) and not "higo de Chiapas"(Mexican fig)? Sure, officially Spaniards thought they were in India.
- The roman eagle eating snakes in 1900 years old https://www.dailymail.co.uk/sciencetech/article-2478904/Roman-sculpture-eagle-devouring-serpent-unearthed-London.html. Is the Mexico eagle eating snakes a more modern version, only 500 years old? https://www.ancientsculpturegallery.com/roman-eagle-mexican-eagle-clutching-the-snake-plaque.html . The ring of a roman legionary showing an eagle eating a snake.
All this, solved with only two genetics analyzes.
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Интересный подход.
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During our lab experiment, we tested 4 different cell stains on squash plant punches. After incubating for 15 minutes, 30 minutes, and 1 hour, checking progress after each marked time, we noticed that even with the green cell stains tested our plant punches appeared red in color from the UV light fluorescence against the chloroplast.
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Evans Blue Stain
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Respected Experts, I have recently join seed company as research and development officer. My company import hybrids of corn, Potato, squash, Cauliflower, and sorghum. My duties are to conduct trials of imported seed on different location as well as management of these trials and selection of best adapted hybrid .You are experienced in this field, Can anyone please share material that will help me to conduct and manage field trials as well as selection of best adaptable hybrid. My email address: asadhafeezpbg@gmail.com. Please guide me experts. Thanks.
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Thanks Sir
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The result of the project was approved by the Agricultural Ministery in 1996. The result was named: Cuban pumpkins, technological variants for their sustainable production.
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Yes but acceptance of this project will confirm on 25th Jan after the defence of this project
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I purchased a softgel containing saw palmetto and pumpkin seed oil. I plan to cut open the softgels and create a topical solution using the insides of the softgel. However I'm having trouble coming up with a way to dilute the contents of the softgel. The inside solution I know is not water soluble.
I was thinking of dissolving it in olive oil. I'm worried about affecting the bioactives in saw palmetto and pumpkin seed. I also would like to increase the skin penetration of the topical.
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use lanoline as diluent , it is with good pentaration capability
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Is there anyone who could provide me information concerning recent developments (protocols) in genetic transformation and regeneration of Squash (Cucurbita peop ..)
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Hy i want ask one question does anyone know that work have been done on absence or presence of Vg gene in Bactrocera dorsalis and Bactrocera cucurbitae regarding egg yolk protein so far i only find paper on B. dorsalis so if some one know please tell me about this status
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You may want to ask Dr. Scott Geib, research entomologist at USDA ARS in Hilo, Hawaii: scott.geib@ars.usda.gov; he has done molecular work with Bactrocera dorsalis:  http://www.ars.usda.gov/pandp/people/people.htm?personid=45241
Cheers, Jaime
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plant pathology, Cucurbita pepo breeding,
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I am agree with Zuo Xianhong and Juan Pablo Manzur. We saw the syptom on fruit. I think this is papaya ringspot virus (PRSV). In Viet nam, this is a problem on Papaya. Best regards.
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Is anyone aware of (preferably peer reviewed) publications on the pollination of Cucurbita pepo by Apis mellifera and Bobus sp. (preferably in in Europe)? I am particularily interested in the effect of pollination on C. pepo seed development and yield.
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Dear Michael:
I only found this article since 1993, relates the pollination of Apis mellifera on Cucurbita pepo. It's the only whose study site is in Europe.
I hope it will be helpful.
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I am developing a guide to heirloom and open-pollinated squash/pumpkins and encountering confusion in definitions and fruits not seeming to match the described characteristics.
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Not available any squash descriptor? Then need to develop new one. Its not that easy. 
better try to fit them in the available descriptor. and then you can mention the differences you observed..
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Recently I tried to inoculate MYSV for seedling screening. Any of you ever work with MYSV? kindly help me. Thanks.
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I work with SVNV. We keep the plants in the dark overnight before inoculating in the morning. We use young plants. I combine mono and di-sodium phosphate in the buffer until the pH is 7.2. I use fresh buffer each time. Add 2-mercaptoethanol to phosphate buffer at 0.1% rate. use this buffer to grind the plant tissue and apply to leaves (that have been dusted with carborundum) gently with gloved finger. Keep buffer and plant tissue cold throughout process. then rinse leaves after a couple minutes. 
With SVNV we only get about a 25% success rate, so I inoculate many plants at a time. It also appears that there may be variations between strains. Maybe this is happening with your virus. 
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Also if you know any procedure...
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If you are interested in the coat-protein genes for WMV-2 resistance, you can find more in Marc Fuchs' paper from Nature in 1995. (Attached)
There are also the naturally occurring genes Wmv from C. moschata and Wmvecu from C. ecuadorensis that have been introgressed into C. pepo. (see Paris and Brown 2005 HortScience link.)
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I found this Lamiaceae with tiny blue corolla on several occasions in dry river beds ('barrancos') close to habitations in Tenerife, Canary Islands. I believe it is a non-native species, introduced with sewage sludge (along with Solanum lycopersicum, Cucurbita spec., Helianthus annuus, Phalaris canariensis, etc.). I guess it either is a spice or a birdseed impurity.
Thank you in advance for your suggestions.
Best wishes,
Filip
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It is in all likelihood an unusual individual of the Mexican species Salvia hispanica L (section Potiles). It is "chia" of the Maya people, a plant widely cultivated for its food seeds.(Abnormal) exaggerated growth of the floral bracts, as it is the case on the ground of the picture, can be associated with herbicides and pesticides for crops. I noticed this phenomenon in an experimental cultivation of these plants (Salvia species) I had at the National University of Colombia. This plant is naturalized in some countries of America.