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Cucumber - Science topic

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The desired plants include tomatoes, cucumbers
Thank you dear friends
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For tomatoes the pollen is sensitive to high temperatures. In summer many tomatoes become male sterile.
I would suggest your ability to make crosses would be improved in greenhouse chambers where lighting and temperature are controlled.
Pollen can be affected by nutrition and complete plant analysis can be combined with drip line and foliar application to optimize the nutrition compared literature values.
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I'm currently researching on Benefits of cucumber marketing so I need ideas and recommended journals for the research. Thank you.
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Please, read this, it is in Spanish:
And this one:
I hope this helps you.
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We are training home gardeners to identify possible cucumber and chayote mosaic viruses and would like protocols that were successfully used in the field.
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As Prof. Ignatov said; it's too hard and complicated identification of plant viruses by symptoms
But in my opinion, the best image collection for cucurbits diseases and their control is this Book:
Compendium of Cucurbit Diseases and Pests, Second Edition
  • A. P. Keinath,
  • W. M. Wintermantel,
  • T. A. Zitter
ISBN:978-0-89054-574-4
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How seeds can be developed in parthenocarpic variety of cucumber under poly houses ?
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Medhat Elsahookie, thank you very much for your valuable support and interesting information.
With regards
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Dear all,
I want to make an edible coating for cucumbers. In most articles, I have seen that the pH of the coating is adjusted to a certain range. For example, the pH of the coating solution was adjusted to 5.6-5.9 for cucumbers. Does anyone know why? Are there any references explaining the effect of coating solution pH on coated fruits?
Thanks
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Zitter, T. A., and J. F. Murphy. (2009) wrote that "The application of resistance-inducing bacterial treatments that may also enhanced plant growth provided protection against CMV, and may be integrated into other pest management schemes. "
Zitter, T. A., and J. F. Murphy. 2009. Cucumber mosaic. The Plant Health Instructor. DOI: 10.1094/PHI-I-2009-0518-01
Is there any chemical agent efficiently suppressing Cucumber Mosaics Virus spreading in the greenhouse?
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Imidachloprid
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I am trying to isolate Fusarium oxysporum from soil/root/stem/seed and a leaf of cucumber. What is the best specific media for Fusarium oxysporum?
I have tried to get the Fusarium oxysporum f. sp. cucumerinum (FOC), from MTCC as a positive control for my research, but I couldn't get it. so, if anyone has the same organism isolated from the cucumber plant affected by fusarium wilt, please kindly answer me. It will be very helpful for my work.
Thanks in advance.
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We have isolated Fusarium oxysporum from clinical and environmental materials on sevearl media, such as potato dextrose agar, Sabouraud dextrose agar and oat meal agar.
We have published two papers on oculomycosis in animals due to Fusarium solani and both papers can be easily downloaded from the Research Gate.
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Please any one help me in finding the Degradation Temperature of the following plants
1. Neem
2. Aloe Vera
3. Cucumber
4. Grapes
5. Almonds
any refrence paper leading to answere my problem..?
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Hi Rizwan,
Generally, medicinal plants do contain active chemicals which are sensitive to heat. Therefore low drying temperatures between 30 and 50°C are recommended to protect sensitive active ingredients.
Depending on major phytochemicals available in the plant material which are needed for further analysis, you can decide the drying temperature. Also considering the duration of heat application, pressure in the drying chamber, the method used in drying herbal material etc. you have to decide it.
For further details, you could refer ;
Thanks.
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Mohammad Salem from the Alexandria University claimed the authorship these three papers:
1. First Report of Fusarium verticilloides causing Banana
Fruit Rot in Jordan
2. First report of cucurbit chlorotic yellows virus from
cucumber plants affected by interveinal yellowing
disease in Jordan
3. First Report of Tomato chlorosis virus Infecting Tomato
Crops in Jordan
You can see these works have been done in Jordan and have nothing to do with Egypt.
Please see the attached file.
Your helping will be highly appreciated.
Best regards,
Nida' Mohammad Salem
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I haven't faced this issue but
Try it, your problem will solve
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I'm planning to silence candidate genes in the root system of C. sativus and viral vectors seem scarce in Cucurbitaceae and with low efficacy. Therefore, I'm looking for people who have experience with VIGS in Cucumber and could indicate which viral strain they used and if they had success. In addition, what is your opinion on new methods such as nanoparticles mediated gene delivery ? Are they promising or a loss of time ?
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Very good question . Though the question is outside my field . I am tempted to raise this question . What is your opinion , these days, many times , any alteration in original gene sequence or genome , application of biotechnological tools like gene editing through CRISPR or RNAi application are often criticised for genetically modified genome as end product , regardless of crop . Is it debatable or an accepted strategy, since many countries still have some stigma with genetically modified crops....
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Bitterness, cucumber fruits, compound
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These symptoms appeard on the top leaves or new young leaves.
Infeced leaves chlorosis along the vein.
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This is a nutrient diffiency symptom
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One of the most important and upcoming research is to find out anti covid 19 plant and plant products. Some people believe that Jack fruit seeds, citrus, consumption of tomato,cucumber may help fighting against covid .
1. How far it is scientifically true ? if any findings kindly share - it can save life.
2. What are the exact benefit from lock down due to covid on Environment from atmosphere, plant to animal kingdom, is it quantified ?
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Glycyrrhizin obtained from roots of liquorice (Glycyrrhiza glabra) may be useful for treatment of COVID-19.
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Protease inhibitors prevent viral replication by selectively binding to viral proteases (e.g. HIV-1 protease) and blocking proteolytic cleavage of protein precursors that are necessary for the production of infectious viral particles. The first step in the activation of NCoV-2 i.e. Covid-19 is proteolytic cleavage of Spike or envelope protein S by protease present in all body cells of humans and more so in wind pipe and lungs. The protease can be inhibited by protease inhibitors present in many natural fruits and vegetables. These include apple, pineapple, resins, cabbage, cucumber, banana etc. Empower your body and immunity to keep up the resistance towards viral infections. Take plenty of warm water. Eat healthy and stay healthy!🙏
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Healthy eating and proper exercise are good ways to keep fit during normal times. However, during the epidemic, maintain personal hygiene, wash hands frequently, pay attention to disinfection, and wear masks to protect ourselves will be more effective.
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Cucumber fruit was rotted at cool storage and fungi had such strange bodies. Can anybody name it?
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There are many fungi that affect cucurbit plants, including many, and they are similar in their symptoms of external infection as Botrytis , Colletotrichum orbiculare and Fusarium oxysporum or downy mildew diseases. Therefore, it is better to grow fungi on a PDA medium or direct slides and the microscopic photo is best for diagnosis.
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The crop is grown in greenhouse
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Thanks a lot Dr. Robert and Dr. Fatima for your valuable answers
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Greenhouse cucumber plants in greenhouse (hydroponics) with normal growth and development have yellow veins on well-developed leaves. What can be the reason: virus or physiological disorder?
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Alex , this looks like typical iron deficiency...
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Hi everyone I am doing PAL assay in cucumber leaves and I am using the paper below as a reference
I don't understand how they convert the absorbance value to enzymatic units
They indicate that they measured the increase in OD290 over a 1 hour period and then they reported as a U/g FW
They add this info:
One unit of PAL activity was defined as the amount of enzyme which caused an increase in absorbance of 0.01 h−1
I hope you can help me Thanks
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I have a question about undetremined plants like cucumber. I want to know how plants can be motivated to finish their growth stage and just countinue their reproductive stage by agronomical practices?
Additionally, in case of hybrid seed production of cucumber, let me know what kinds of nutrients are essential for filling seeds stage? What fertilizers are necessary?
Best regards,
Shahram
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Dear Shahram
From my point of view, P and micrinutrients are essential but you should be sure about other factors such as soil moisture.
Regards
Weria
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Does anyone know a good protocol to isolate RNA from cucurbitaceous seeds? We are trying to detect viral RNA in seeds by using the silica RNA extraction method but we cannot see any RNA when the samples are electrophoresed in 1,5% agarose gel. Any ideas?
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Cultivars to resistant ot root-knot nematodes
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Cucumber had most sensitivity to root- knot nematodes infection especially meloidgyne incognita I had that work before
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Cucumber under protected or open field is attacked by this disease. Infection by this causal might com over the whol plants within days.
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It looks like angular leaf spot to me, see the link below.
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A company in France is selling recordings (protéodies) of a very special music that is suppose to protect plants against certain kinds of diseases. There are also claims that positive results have been obtained for the protection of vines and cucumbers.
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I have not contacted yet people of this company. You are right, if it really works why not publish their results (with appropriate controls) to make it public and not just an article written by journalists?? This science is called (in french) génodique. Have a look at Sternheimer J. musique des particules éémentaires Comptes rendus de l'Académie des Sciences Paris II, 297: 829, 1983. and Henty M. Ondes d'échelles (on line) 2016. and also RG
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Pseudomonas syringae pv. aptata is a leaf blight/vascular pathogen of sugarbeet, sunflower, cucurbits, lettice, etc. It was isolated from cucumbers (CABI report). Do you know any reports about Pseudomonas syringae pv. aptata outbreak on cucumber in hydroponic greenhouse?
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Frank, thank you! I have found this report and some others about P. aptato on cucurbits in field, but not about greenhouse cucumber. Some colleagues said that strains from squash were not virulent for cucumbers.
Alex
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I am working on parthenocarpy hybrid development in cucumber (Cucumis sativus ), and required a effective methodology to calculate degree of parthenocarpy in cucumber.
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We can calculate degree parthenocarpy of cucumber (cucumis serious L) whith calculate number of cucumber flowers.
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I want to check the effect of different residues as soil amendment on plant growth. different treatments of residue will arrange complete randomized design, under plastic tunnel greenhouse. Air dried residue will apply in potted soil and will transplanting the cucumber seedling in amending soil to check the effect of treatment with different concentration on pant growth. What should be the ideal conditions under greenhouse?
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Pay attention to air humidity, It may cause a lot of deceases. Day/night temperatures is very important for fruit set.
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Presence of seed is one of the major problem in acceptance of cucumber, so how we can proceed towards the development of parthenocarpic cucumber, is it possible to develop through traditional breeding method.
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This paper discussed about partenocarpy in cucumber
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Need the expert opinions and suggestion on adopted cropping system. I was little confused to identify the proposed cropping system in my experiments.
Cucumber double cropping system is a typical intensive production model in China with winter–spring (WS) season and an autumn–winter (AW) season annually under plastic greenhouse vegetable cultivation (PGVC) structure.
In our experiments, we have grown different over winter leafy vegetables in the same land during off-season cucumber cultivation, eg. during summer fallow period (October, 2016), and summer fallow period was usually between WS and AW season. we supposed to grown leafy crops for soil quality and microbial community response. or check the effect of leafy crops on seasonal cucumber yield and soil quality.
We harvested the leafy vegetables after short-term fallow period (mid Feb,2017), and cucumber again cultivated for WS and AW season in same greenhouse field.
Please suggest that which type of cropping system or practices or cropping model we adopted during double cropping system?
is it called as;
1: leafy vegetables used as cover crop rotation?
2: this practice belong to multicroping system ?
3: relay cropping system?
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As Jonath Alumona has aptly remarked the double cropping of cucumber will be considered a monoculture and as monocultures favor adaptation of pests and disease are double crop rotation might be more useful. The use of cabbage or broccoli established as the autumn crop and using as a follow the cucumber provides rotation. To keep a summer crop the use of sweet potato may be tried as a triple cropping system. White plastic mulch might be best for the summer and autumn crops and black mulch for the spring cucumbers. Spring cucumbers can be a short duration variety, as well as summer sweet potato, and fall cabbage and broccoli might also be focused on short duration crop sequences. The cucumber is cucurbit, the sweet potato a convulacae, and cabbage or broccoli a brassica. This more extended rotation will help prevent the adaptation of pest and diseases as well as composting all wastes with manures and high temperature to provide nutrition and eliminate disease and pests.
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We have a cucumber wilt that is spreading in greenhouses.
I have done some search online and found similar cases in the area (I posted the photos that is similar to the case we have).
Please share your opinion.
Thank you,
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I am using the curve fitting method of Moualeu‐Ngangue et al., (2017) on A-Ci curves measured on rice plants. However, in their linked Excel spreadsheets, Ko=180 (millimole/mole) and Kc=373 (ppm) values for cucumber rubisco are used.
Looking through literature for values relevant to rice, I found two contrasting sets of values,those of Makino et al., (1988), Kc=245 ppm, Ko= 307.7 mol/mol (values originally in micro Molar) Perdomo et al., (2016), Kc =300 ppm, and Ko=471 mmol/mol (values originally in pascal and kilopascal respectively).
First of all, this is surprising because of the large difference with the values used for cucumber, and secondly which of the Km sets for rice should be used? *There is a possibility a mistake was made in the conversion-using Henry's law- from the original values to the preferred ones.
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Great, if your calculation is under a different air pressure, then it makes sense.
As for the Kc and Ko, actually to me, the difference is not "so large". An accurate number would be difficult due to several reasons.
1) most Kc and Ko come from in vitro measurement, and it's quite different environment in vivo.
2) As you mentioned, small difference in the structure of Rubisco. Mutation on several loci already can change the affinity significantly. So it is reasonable to vary by tens of ppm for Kc.
Anyway, we still can obtain an "accurate" Farquhar model even though the Kc and Ko are biased. The deviation caused by Kc and Ko can be mostly compensated by Vmax and gm. To me, those biased numbers would still be useful for a comparison study done by the same protocol and same person :P
And another thing I'd like to mention is that I looked into the paper Moualeu‐Ngangue et al., (2017), in my opinion, I would say this method is not robust.
For example, in its attached excel worsheet, the best fitted results a SSE=4.233.
It's very easy to find other combinations of {Vcmax,J,Rd,Tau} which results a very close SSE. I listed two I found {Vcmax=163,J=150,Rd=0,Tau=0.55,SSE=4.515} and {Vcmax=120,J=121,Rd=0,Tau=0.44,SSE=4.952}. Of course, gm in the worksheet also varies a lot. Therefore this estimation is not stable. So be careful with the number you get from this method.
Best regards,
Yi
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I am trying to use Isaria fumosorosea as a biological control for Aphis gossypii in a greenhouse for organic cucumbers. When I mix the spores with water to spray on the plant, most of the spores are on the bottle wall. What would be the best solution to apply the spores by spraying?
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You've tried adding a drop of Tween-20? That works for hydrophobic spores, although it can be fungitoxic. There was a time when we sprayed spores in liquid Freon, but that might be illegal now. Depending on your spray rig, you might be able to use a 0.01-0.02 % water-agar solution, which holds spores more uniformly in suspension.
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with simple scale measuring its length and width and addition of correction factor is reliable or not?.
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Hi my friend
To measure the area of a leaf
First weighs a paper at a specified size (5 cm * 5 cm = 25 cm2)
Then cut the paper to the size of the leaf and weigh the piece
The area of the leaf is based on the weight of the paper.
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Hypothetical Scenario:You observed that lactic acid bacteria are capable of growing in fermented cucumber juice medium if the pH is adjusted from 3.7 to 4.7, without supplementing the medium with alternate energy sources. It is hypothesized that in your experiment, variability in the medium composition may have caused differences in lactic acid bacteria growth rate and maximum cell density and may have impacted the secondary fermentation biochemistry.
Question 1:Imagine that you have to run the same experiment again and design an enhanced protocol to answer the hypothesis described above. The goal of such repetition would be to eliminate the interference caused by the variability in the medium composition. Describe the details of the new experimental protocol and justify every parameter
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Jai Ghosh thanks for your answer Dr. Ghosh, it is very helpful.
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The Soil Science Society of America (SSSA) will host scientists, professionals, educators, and students at the SSSA International Soils Meeting. "Soils Across Latitudes" will be held Jan. 6-9, 2019, in San Diego, California in collaboration with the Canadian Society of Soil Science and the Mexican Society of Soil Science. I have invited as poster presenter with following work details.
Presentation Information: Session Title: Meeting the Challenge to Develop Sustainable and Resilient Agricultural Systems By Understanding Soil-Plant-Microbiome Relationships Posters Session Date/Time: January 08, 2019, 4:00 PM - 6:00 PM
Abstract Title: Co-amended synergistic interaction between AMF and organic substrate induced cucumber yield and fruit quality associated with regulating AM-fungal community structure under anthropogenic PGVC soil.
Presenting author affiliation: College of Horticulture, Northwest A&F University, Xi'an, Yangling, China
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Good information. Thank you...
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QA Automation Tools such as cucumber, selenium, etc check the quality of code. Is there any tool available that provides knowledge regarding software quality requirements (a.k.a. Non-functional Requirements) analysis at early stage of Requirements Engineering.
Please share your experience.
Thanks
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No such tools are used for quality requirements ...but some methods are used..
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I have cucumber plants with aphids and a few of the aphids have been parasitized. I collected the parasitized aphids and after 10 days, this wasp has come out. I have tried to identify him and have come to the family Braconidae. Can someone tell me if it is the right family?
Here is the photo of the wasp.
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The list of Mackauer and Stary (1967) is very outdated with more than 51 years old, incredible as someone recommends a bibliographical reference so old. The CABI 2018 list that I recommend is the most updated and the most complete containing all the parasitoid species so far reported parasitizing Aphis gossypi.
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Dear colleagues,
We cultivate the zucchini & marrows at the greenhouse. The full area for this species is too much for us. We are interested to cultivate zucchini with any other vegetable. All our efforts for acclimatization of any vegetables (cucumbers, peppers, tomato, eggplant) were unsuccessful.
Could you be so kind to inform us about vegetable, compatible with zucchini?
Sincerely,
Mehdi.
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Mehdi Ali,
peppers, tomato, eggplant are not compartible with cucurbits in humidity/temperature balance , cucumber is more susceptible to the same pathogens that infect zucchini. you can test other cucurbits.
Alex
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I am working on Arbuscular Mycorrhizal Fungi (AMF) with emphasizing on Cucumber productivity in the presence or co-inoculation with organic substrate, Could someone guide how to check the effective AMF inoculation% ?or suitable staining techniques to visualize the AMF roots interaction with substrate .
best staining procedure.
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Procedure
  1. Place roots in holders so that your sample fits in the lower 50% of the container. Commonly used containers are scintillation vials and test tubes of various sizes. Aside from the size, the only other consideration is that the container is made of a material that is autoclavable. If you are processing many samples, you may want to cover the containers with snug-fitting mesh so solutions can be easily changed out. Open lids (with mesh in them) may be purchased for 40mL scintillation vials.
  2. Add 10% KOH (w/v) to each container, making sure that the sample is completely covered and that the fluid does not fill more than half of the container. For field grown roots, you may soak the tissue overnight, making sure to replace with fresh KOH before the heat treatment. You will find that the KOH turns yellow overnight, indicating that tannins have left your tissue and gone into solution. The overnight soak may be done prior to or following heat treatment. In some tissues two periods of soaking may be necessary.
  3. The heat treatment may be via boiling in a waterbath, or by autoclaving. This is the part of the procedure that most needs optimizing. For greenhouse-grown maize roots 2-3 weeks old, only 15 minutes of autoclaving (with no prior KOH soaking) is necessary. Field grown adventitious roots of maize that are 1cm in diameter and 2-3 months old require overnight soaking and 1 hour of autoclaving. Some tissues may require multiple soaking and more than an hour in the autoclave. Boiling time is generally longer than corresponding autoclave time. Roots that have been well cleared resemble cooked rice noodles. They are both translucent and transparent, and are white (not even remotely yellowish!). Remember that clearing with KOH removes many compounds in the cell wall, so after this process, your roots will be mushy and flimsy. Lateral roots may partially or completely disintegrate. Caution should be taken not to dissolve parts of the ground tissue (the cortex usually goes first). It is difficult to ascertain if roots have been completely cleared without staining, but examination of cleared and unstained roots under 4x magnification of a light microscopy will reveal white tissue composed of cells surrounded by thin grey cell walls. Also, you should be able to focus through the sample.
  4. Remove samples from autoclave and rinse in tap water three times. Add 5% HCl and let it sit for about a minute. Pour out the HCl and add trypan blue stain. Let it sit overnight (or longer is fine as well), and examine the roots under a dissecting scope. If the roots are very dark blue, they did not clear long enough in KOH and further optimization is necessary. Stain: 1 L glycerol 950 ml distilled water 50 ml acetic acid 0.2 g trypan blue
  5. The typical method of quantifying colonization of the root is by the gridline intersect method (McGonigle et al, 1990). This method has been adapted many times, and each investigator should consider optimizing these examination and quantification procedures to suit desired data collection. Below are a few possible approaches.
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I have a cucumber farm and I want to take record of the depth of irrigation so I want to know how best to do so if it's direct on the farm or in a Vase
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Neutron probe method
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Cucumbers are high in water and low in calories, fat, cholesterol, and sodium and are high in antioxidants which allow your body to function optimally.
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Macroscopically, check the colouration and you can use 3M kits, fasting method as mentioned by Eshak.
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There is a strong deformation of plant tops, leaves, fruits crack along and filled with smelly liquid. The cross-and longitudinal sections free of any symptoms of systemic infection. More than 1000 diseased plants were found per hectare.
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Agreed with Bozena.
just wanted to ask is there any incidence of pests like aphids or jassids. Pests can also causing the leaf curling and spread of virus. But by the pics, the first explanation would be water stress.
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I hv a polyhouse in experimental field. I hv experimented parthenocarpic cucumber inside it during winter n got bumper production. But now in march the temperature in my area touches 49 degree n it ll go up to 45 degree in april -may. Now my cucumber crop is over... I want to utilise my space inside the polyhouse In summer too but I can't understand which crop shall I take in this climate... can u all plz suggest me some......
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You can grow any summer vagetable , provided you have dehumidifier installed in polyhouse....
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What extent vermicompost would be desirable for mixing in cocopeat to be used for hydroponics cultivation? This is in view of probable consequences of degradation by decomposition of cocopeat that would determine the overall life of cocopeat.
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Your welcome Dr. Kumar
Be successful
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I am working in a project with identifying cucumber fruits in a green house environment. i have followed some research articles and found K-means clustering for segmentation process. defining the initial solution is measured in randomly input data and the results will change time to time. Is there any better method to identify initial other than using random data.
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Thank You
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I have incorporated fresh garlic stalk into replanted soil in pot experiment examining cucumber growth and soil quality. Before application tested the soil and garlic residue properties. we found 444.43 C:N ratio of garlic stalk. can you recommend as good quality crop residue? and more explanation at that CN ratio.
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Very interesting question indeed. Any organic residue of such high C:N ratio will not be able to benefit cucumber, especially in a pot experiment, but surely it will aid in improving the physical properties of the treated soil. Release of nutrients in from organic residues can be accelerated by adding some nitrogen to narrow down C:N ratio.Unless C: N ratio buffers around 20-30:1, direct implications of organic residues on changes in soil fertility will be difficult to be realised in such short duration crops.Residual effect of such residues will be increasingly felt. Soil properties and their baseline microbial diversity also play important role in release of nutrients through such crop residues...
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Leaf blight has appeared in greenhouse in 3 days after rain - some droplets came in through open windows. The blight symptoms are spreading along leaf veins.
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I think it is a physiological reaction of the plant to the pH of the substrate.
Also, I think it is correlated with deficiency of potassium, light, temperature and atmospheric humidity. You have to check.
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we start harvesting cucumber and we have trips infestation. 
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united Arab emirates
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Cellulase production, agro waste
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Thank you Garcia
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I need publications on the occurence of bacterial soft rot diseases of cucumber caused by Pectobacterium carotovora?
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See
Cui et al "Methane-rich water induces cucumber adventitious rooting through heme oxygenase1/carbon monoxide and Ca2+ pathways"
for clarification of what is meant by 80% saturation.
Thanks
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You may firstly calculate the saturated concentration of dissolved methane (at standard state) out, via an equation of temperature and salinity. The later can be found in Table A2 of Wanninkhof (1992). And then you could work out the methane concentration via the saturated concentration and the saturation value.
Reference
Wanninkhof, R., 1992. Relationship between wind-speed and gas exchange over the ocean. J. Geophys. Res., 97, 7373–7382.
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Hello,
I am testing endophytic bacteria in biocontrol of greenhouse cucumber powdery mildew (adapting hydroponic system). In one of these trials, I experienced a hypersensitivity response from some leaves by developing brown wrinkled lesions taking half or more of the leaf area. It is not a necrotic lesion, mostly, look like a hypersensitivity response. My question, how can you score these lesions?
Thanks in advance
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I know this is not powdery mildew infection and it is an extra infection I experienced in some replicates during the greenhouse trial. 
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Obtaining the viable vigorous seeds of vegetables particularly bell pepper and cucumbers
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I do not follow your question. However, when I was working with cucumber and other cucurbit genetics, I put a paper bag over the young fruit. The fruits receiving this treatment came through, whereas some of the ones not covered either rotted because of insect holes, or diseases which seemed to be associated with soil contact.
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I want to prepare leaf discs of cucumber in agar medium in petri dish so that just leaves stay fresh to 48 h. Which Agar is better?1-Agar agar 2-plant agar 3-bacteriologic agar
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Dear M. Alizadeh
To prepare the leaf discs around 30 ml of (1.5% agar solution was poured into a 7 cm Petri dish) after cooling the agar (simply agar)  leaf in this media and condition was able to survive 10 days, all tool used in cutting the leaves were sterilized in 90% ethanol to reduce contamination. I was able to keep the leaf for at least 10 days simply. I add parafilm covering over the dishes.
Hoping this will be helpful            
Regards
Prof. Houda Kawas
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Hello everybody,
Downy mildew disease is a common problem in cucumber. The initial symptoms in cucumber seem to be angular or blocky, yellow areas visible on the upper surface of the leaves, when the lesions mature, they enlarge rapidly and turn brown. The underneath surface of infected leaves appears to be water soaked. During the disease-favorable conditions, downy mildew will spread quickly, destroying cucumber leaves within few days. Severe infection results in leaves that are totally dead and curly up, causing major economic losses to the farmers. I would appreciated very much,if you kindly mention any suitable method (biological control) to get rid of this disease.
Jawad
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Dear Prof. Dr. Jawad, 
I agree with Assist. Prof. Ahmed.
You also can see the attached articles :
Best greeting
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I am interested in how to determine the degree of parthenocarpy like cucumber. Some references say that in greenhouse, low temperature and without pollination are conditions to produce parthenocarpic cucumber fruit. But they do not tell us how to decide the degree of parthenocarpy, to which extend the degree is high or low. Thank you! 
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Some cucumber varieties grown under  green house conditions in US and some parts of Europe are parthenocarpic.  Middle East and some East Asian countries also cultivate seedless varieties. But majority are cultivated through seed. Apart from Cucumis sativus and C. melo flexuosus, Marah oregana is also a cucumber ; western wild cucumber. One can search the literature for this scientific name  and may get some inputs.
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Methyl salicylate has been known to release by many plants when infected with different insects, cucumber when infected with mite also releases methyl salicylate, does any paper cited that when cucumber infected with cotton aphid could release methyl salicylate?
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Hello Dear
Methyl salicylate, an ,has been shown to attract natural enemies and affect herbivore behavior. Best regards
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Research Guidance for my field experiment under plastic tunnel. Our hypothesis of this research is growing overwinter leafy vegetables as a crop rotation during off-season cucumber growth  for two year under continuous cropping regime in order to reduce the excessive application of chemical fertilizers in Cucumber production under plastic tunnel cultivation. What would you suggest some soil indexes for 4 growing seasons of cucumber?
In another Experiment. I am working on applying plant residues (garlic stalk) and AMF fungi for Cucumber growth in Continuously 2 years soil-plant study under plastic tunnel. What would you suggest for me some important relevant soil indexes and physiology indexes?
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Before starting such studies soil index such as fertility status, reaction etc. is needed to be estimated and accordingly nutritional studies are planned. So far as physiological index is concerned, on the basis of objectivity of your studies, plant growth and development parameters of plants in poly tunnel need to ascertained for recording data.
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I am working on gynoecy and parthenocarpic expression in cucumber where gene sequence information is available. Now looking for the ideal marker and approach for designing the primer.
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 I agree totally withnDr, Weston Testo's suggestions
Dr. Srinathrao 
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We intended to produce seeds from seedless cucumber cultivars, can someone assist please
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I am trying to find out how to store sclerotia from Sclerotinia sclerotiorum. I have three isolates from cucumber, sunflower and squash, can anyone advice on how to store this properly if I want to culture it in the future?
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Hi  Pfano : The best way to preserve the fungi is to keep the most structure that can survive to long term, for the Sclerotinia sclerotiorum the best is to preserve the sclerotia, you can preserve it as dry in room temp for more than 1 year , but if you want to preserve for long term dry the sclerotia and keep it in vial at 4C or -80C.  Please find the attached file for the useful paper.
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how farmer can produce the seed of seedless cucumber for multiplication and growing for next season?
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Mr. singh 
  you can spray Ag No3 (300 ppm) @ 3-4 leaf stage and 15 days after 1st spray he may get 10% of male flower in seedless cucumber, if its gynocecious line,or if its monoecious  line you can directly self it.
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I want to grow cucumber under salt stress but can anyone help me in suggesting the best specie of AMF? 
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Please, take a look at this paper:
E. Gamalero, G. Berta, N. Massa, B.R. Glick and G. Lingua
Interactions between Pseudomonas putida UW4 and
Gigaspora rosea BEG9 and their consequences for the
growth of cucumber under salt-stress conditions
Journal of Applied Microbiology 108 (2010): 236-245
doi:10.1111/j.1365-2672.2009.04414.x
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Dear Scientists,
 I investigates ecology of wild cucumber (Echinocystis lobata) in second range. 
I have a huge request, Could someone send me a fruit of this species of NATIVE range (USA). 
I'm grateful for the sending of 200 - 300 fruits (fruit can be dry), a minimum of 4 posts. I give shipping address on priv. I will pay for delivery. 
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Thank you!
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Effect of foliar application of boron, zinc, and iron on growth and yield of cucumber in polyhouse condition. Levels of each micronutrient (Boron, Zinc and Iron) are three (0, 25, 75).
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Dear Bhim,
As you stated, you want to do your experiment at polyhouse condition. Design of your project is changed according to the some factors; irrigation system, plantation from pot or land... If seeds of cucumber are planting directly from land is better to follow of RCB Design.
Kind regards,
Rabar,
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I am doing a research on cucumbers (Cucumis sativus) so I am looking for some research in this area.
thanks for your kind help 
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Hi Arshad
I hope these publications will help you
1- "Effects of sowing date and pinching on broccoli seed production"
(A. A. El-Yazied et al., 2007)
for a copy please send a message to my friend hany gamal on his researchgate page
2- Effect of head decapitation and planting density on quality seed production
of sprouting broccoli (Brassica oleracea var. italica L.)
(Mehta et al., 2015)
download this link
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If there is a need to analyze the sample for nutrients such as N or protein, the temperature should not exceed 65C. Dry until constant weight is achieved.
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Now I research on select useful cucumber cultivars to control cotton aphid to reduce using pesticide, I don't know constitute or induced plant defense is better, or plant could also has two kind of defense?
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Both the defenses play an integral role in HPR and it all depends which is working in your case. Generally against sucking pests, its the induced response which is more pronounced.
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Hi 
I hope you have a good time
I wonder if someone can diagnose the cause of disease on cucumber?
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Dear  Remi Cornwall
Thanks a million
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Hi
I hope you have a good time.
As you know the part of cucumber's stem has been discolored. 
could someone say to me what the cause of this problem is? 
 I really appreciate it.
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Hi,
I can not see the root system clearly in the picture but seems to me healthy. This kind of symptoms can be seen with abiotic factors like phytotoxicity. Did you sprayed anything on plant? Otherwise you need to cut a little part of stem just below the yellow part longitudinally or cross and can see if there is any kind of discoloration. If yes there is chance of Fusarium infection. Very prevalent in cucumber. Also you need to collect more information such as, did you over-watered them, or any kind of spray, added fertilizer and also is there any discoloration/ injury in roots you found. Hope it will help.
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My project is Somatic Embryogenesis in Cucumber. I need your help. Callusing treatments are great, but I can not steer them towards embryogenesis. Callus induction hormone treatments containing different concentrations of NAA and BAP.
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Thank you Thom
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An attempt is going on to rear and release Chrysoperla carnea larvae for controlling vegetable (tomato and cucumber) pests, mainly Bemisia tabaci, Aphis gossypii and Tuta absoluta, in green houses. We would like to know to what extent that this approach can substitute chemical insecticides for controlling such pests, and what stage of the predator is more appropriate for releasing ?. Your advice and relevant literature will be highly appreciated.
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Dear Abdalla Abdelrahim Satti, 
 Biocontrol works best when you rely on established procedures. The biocontrol agent producers have invested many years of research to offer these procedures. They give you very detailed informations concerning the necessary prey densities, the moments of release, the techniques of release (to avoid the activities of ants),  and the dosage of predators or parasitoids. They have developed wrappings that avoid cannibalism and they experimented the number of release points that conduct to satisfying and reliable results. Furthermore, they have developed in International Working Group 5-Year Projects all that quality control experiments and procedures to assure that the population you release is able to assume the job they are released for. Having all these data not only published in literature but assured by biofactories, I cannot understand, why people return to the beginnings and try to do it themselves again and again. It is not less expensive to invest all those hours to reinvent all of this, than to apply the products that are existing in the markets. Perhaps you can pass a technical semester in one of the biofactories IBMA represents worldwide, to discover all of these procedures. Wish you the best of luck,
Carola Meierrose 
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Hello everybody. I want to measure some enzymatic parameters of Aphis gossypii and for this end I used a specific cultivar of cucumber “Soltan“. For some reasons, I prefer to change my cucumber cultivar, but as some experiment have been done using previous cultivar. Now I want to know if changing the host plant in the level of cultivar has any significant impact on the rest of experiments, considering this fact that I want to compare my new results with ones obtained with previous cultivar.
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I have stored my cucumber seeds treated with brassinoids at -20 °C for more than 15 days. Will there be still effect of brassinoids on cucumber seeds or I have to treat it again.
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What is your aim? why not tae out some of the seeds and go for a germination test?