Science topic

Cryobiology - Science topic

Cryobiology is the branch of biology that studies the effects of low temperatures on living things within Earth's cryosphere or in science. The word cryobiology is derived from the Greek words cryo = cold, bios = life, and logos = science. In practice, cryobiology is the study of biological material or systems at temperatures below normal. Materials or systems studied may include proteins, cells, tissues, organs, or whole organisms. Temperatures may range from moderately hypothermic conditions to cryogenic temperatures.
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Publications related to Cryobiology (1,106)
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Dimethyl sulfoxide (DMSO) is a cryoprotective agent that is used in cryopreservation of biological cells. Cryopreservation is a way to store biological material without degradation at low subzero temperatures (often -80°C or -196°C). Although DMSO represents a standard cryoprotectant used in medical practice [1], mechanism of its cryoprotection is...
Conference Paper
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Trehalose is a promising cryoprotectant for the long-term storage of mesenchymal stem cells (MSCs). Trehalose is relatively safe for the human body and a potential substitute to reduce the toxicity of dimethyl sulfoxide (DMSO), a common cryoprotectant. Previously, we developed an alginate-chitosan microcapsule containing human stem cells. However,...
Article
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Cell monolayers underpin the discovery and screening of new drugs and allow for fundamental studies of cell biology and disease. However, current cryopreservation technologies do not allow cells to be stored frozen while attached to tissue culture plastic. Hence, cells must be thawed from suspension, cultured for several days or weeks, and finally...
Conference Paper
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Boar sperm is associated with the post-thawed sperm quality reduction due to the low cholesterol and high polyunsaturated fatty acid contents in their sperm plasma membrane (Li J, 2017, Cryobiology, 80, 119-125). The objective of the study was to investigate the efficacy of the cryoprotectants combination at different concentrations on the Windsnye...
Conference Paper
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Chyzh M., Koshurba I., Hladkykh F. A study of antiulcer activity of cryoconserved placenta extract on the model of alcohol / predisolone-induced stomach lesions. The 59-th Annual Meeting of the Society for Cryobiology «CRYO – 2022» (2022 19–22 July, Dublin, Ireland). 2022. Р. 164–5. DOI: https://doi.org/10.5281/zenodo.6865370. /// Search the new a...
Article
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Technologies to cryogenically preserve (a.k.a. cryopreserve) living tissue, cell lines and primary cells have matured greatly for both clinicians and researchers since their first demonstration in the 1950s and are widely used in storage and transport applications. Currently, however, there remains an absence of viable cryopreservation and thawing...
Article
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Cryopreservation of pollen is a simple and effective method of longterm pollen storage and conservation of genetic resources in agriculture and forest genetic resources.it is an effective technique to overcome asynchronous flowering and conservation of important alleles.it is the method in which the pollen is stored in liquid nitrogen for many year...
Article
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Assistive reproductive technologies are increasingly used to control the biology of fertility and its temporality. Combining historical, theoretical, and socio-empirical insights, this paper aims at expanding our understanding of the way temporality emerges and is negotiated in the contemporary practice of cryopreservation of reproductive materials...
Book
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This is a special issue of the journal Animals. All articles are available at: https://www.mdpi.com/journal/animals/special_issues/Assisted_Reproductive_Techniques_and_Germplasm_Cryopreservation_Applied_to_Wildlife Many wild species are difficult to reproduce naturally ex situ. Behavioral, physical or physiological issues may be involved. Furtherm...
Thesis
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Hibernation is an energy-saving strategy employed by species to survive periods of low resource abundance and inclement weather. Due to the costs and benefits associated with the use of torpor and arousal during hibernation, individuals are predicted to hibernate according to their energetic state entering hibernation and their requirements followi...
Conference Paper
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Chyzh M, Koshurba I. Comparative evaluation of antiulcerogenic action of cryoconserved placenta extract under different modes of introduction. Abstract book of the Annual Conference of Young Scientists "Cold in Biology and Medicine – 2022"; 2022 May 25-th; Kharkіv. Kharkіv: Institute for Problems of Cryobiology and Cryomedicine of the National Acad...
Article
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Germplasm storage and transportation in artificial insemination (AI) and other advanced technologies are facilitated by cryopreservation. In reproduction, the cryopreservation of sperm allows it to be transported across vast distances and used even after the sire’s death. However, the technique of cryopreservation might damage sperm and limit their...
Article
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Sperm cryopreservation is vital in combating the human infertility, but regrettably, the toxicity of cryoprotectants and the occurrence of intracellular icing, osmotic shocks or shrinkage of the cells below a given threshold volume greatly affects the success rate of this technique. Using the virtue of nanotechnologies and depositing water-repellen...
Article
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Whole-animal, in vivo protein metabolism rates have been reported in temperate and tropical, but not Antarctic fish. Growth in Antarctic species is generally slower than lower latitude species. Protein metabolism data for Antarctic invertebrates show low rates of protein synthesis and unusually high rates of protein degradation. Additionally, in An...
Article
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The effects of different preservation temperatures two cryoprotectants (glycerol and DMSO) at two concentrations (5% and 10%), followed by two different freezing methods (rapid and slow freezing) and two different preservation temperatures (-80°C and-196°C) on the viability of Vero cells were examined in this study. Vero cells were preserved for 18...
Article
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Cryopreservation is globally used as a method for long-term preservation, although freezethawing procedures may strongly impair the gamete function. The correct cryopreservation procedure is characterized by the balance between freezing rate and cryoprotective agents (CPAs), which minimizes cellular dehydration and intracellular ice formation. For...
Article
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Significance Recent studies on liquid water suggest that the two liquid waters exist in the supercooled temperature region and that their existence relates to the anomalous behavior of low-temperature liquid water such as the maximum density at 4 °C. However, the experimental investigation of two liquid waters is difficult because of the rapid crys...
Chapter
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The development in cryobiology in animal breeding had revolutionized the field of reproductive medicine. The main objective to preserve animal germplasm stems from variety of reasons such as conservation of endangered animal species, animal diversity, and an increased demand of animal models and/or genetically modified animals for research involvin...
Article
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In this paper, we present the formulation and a method for solving the problem of freezing living biological tissue with a flat circular cryoapplicator. The model is a two-dimensional boundary-value problem of Stefan type with nonlinear heat sources of a special type that provide the actually observed spatial localization of the temperature field....
Article
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Understanding hibernation in brown bears (Ursus arctos) can provide insight into some human diseases. During hibernation, brown bears experience periods of insulin resistance, physical inactivity, extreme bradycardia, obesity, and the absence of urine production. These states closely mimic aspects of human diseases such as type 2 diabetes, muscle a...
Article
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The value of cryopreserved germplasm in agriculture, aquaculture and medicine was recognized in the mid-twentieth century following the discovery in the late 1940s of a method for recovering viable spermatozoa after freeze-thawing. Sir Alan Parkes (a founder of cryobiology as a discipline) remarked that "time and space has been abolished for cattle...
Article
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Studying reproduction in wild animals is complex as there are as many biological traits as there are species. What we currently know is minimal compared to the large number of species that remain unstudied. In addition to the impressive diversity of natural mechanisms, other complexities limit the progress of wildlife reproductive science-little in...
Article
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Differentiation of human induced pluripotent stem cells (hiPSCs) generates cell phenotypes valuable for cell therapy and personalized medicine. Successful translation of these hiPSC-derived therapeutic products will rely upon effective cryopreservation at multiple stages of the manufacturing cycle. From the perspective of cryobiology, we attempted...
Article
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Cryopreservation is known as an applied aspect of cryobiology or the study of life at low temperatures [...]
Article
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The value of cryopreserved germplasm in agriculture, aquaculture and medicine was recognized in the mid-twentieth century following the discovery in the late 1940s of a method for recovering viable spermatozoa after freeze-thawing. Sir Alan Parkes (a founder of cryobiology as a discipline) remarked that "time and space has been abolished for cattle...
Book
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The book deals with alpine ecology with a focus on high altitude habitats form the upper montane to the nival zone. It is, in general, addressed to the wider public but is as well thought to be informative for the advanced reader. The emphasis is on relationships and the interplay between alpine landscapes, alpine organisms and humans. The book enc...
Thesis
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Hladkykh F. V. Use of cryopreserved placenta extract for correction of ulcerogenic action of nonsteroidal anti-inflammatory drugs (an experimental study). Qualification scientific work published as manuscript. Thesis for the degree Doctor of Philosophy in «22 – Health Care» in specialty of «222 – Medicine». Institute for Problems of Cryobiology and...
Article
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Despite the opportunity to manipulate the water freezing via superhydrophobic materials, their commercial use for passive icing protection is still questioned, since the combined effect of surface morphology, air cushion arrangement, roughness, chemistry and film thickness on the icephobic properties of a given non-wettable solid remains unexplored...
Article
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The carbon soot has generally negative connotation due to the adverse impact on the human health and ambient environment. Most of the studies involving soot analysis focus on the need of preventing or reducing the sources of soot production. However, if the atmospheric soot aerosols are capable of repelling the ambient water vapor and thus impeding...
Preprint
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I describe a new approach to the classification of snow crystal morphologies that focuses on the most common growth behaviors that appear in normal air under conditions of constant applied temperature and water-vapor supersaturation. The resulting morphological structures are generally robust with respect to small environmental changes and thus sho...
Article
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Aim. To evaluate the effectiveness of the use of nanomaterial in the environment for the further development of in vitro embryos derived from frozen-thawed oocytes in the system of conservation of genetic resources of animals at the cellular level. Methods. Biotechnological, cryobiological, morphological, cytogenetic, and statistical methods, as we...
Article
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Ice nucleation on the surface plays a vital role in diverse areas, ranging from physics and cryobiology to atmospheric science. Compared to ice nucleation in the bulk, the water-surface interactions present in heterogeneous ice nucleation complicate the nucleation process, making heterogeneous ice nucleation less comprehended, especially the relati...
Article
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The popularity of nanoparticles (NPs) is continuously increasing. To date, however, there has been little research on the application of NPs in plant cryopreservation, i.e. storage of tissues in liquid nitrogen (LN). The aim of this study is to analyze the effect and evaluate the usefulness of gold nanoparticles (AuNPs) in regard to cryobiology stu...
Article
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The recent advances in sperm cryopreservation transcend cryobanking and other assisted reproductive technologies. Since its discovery, cryopreservation has contributed positive impacts on animal breeding as well as in genetic exchange, improvement, and conservation efforts. However, cryoinjury and variabilities in cryopreservation outcomes remain a...
Conference Paper
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Hladkykh FV, Chyzh MO. The effect of cryoirrigation and cryopreserved placenta extract on the content of nitrogen monoxide in the gastric mucosa in rats with diclofenac sodium-induced gastropathy. The 58-th Annual Meeting of the Society for Cryobiology «CRYO 2021», 21–23 July, Chicago. 2021; Chicago. USA, 2021. p. 97–8. ____________________________...
Conference Paper
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Report at Applied Cryobiology – Scientific Symposium on Cryonics, Dresden, October 4-5, 2014 video: https://www.youtube.com/watch?v=OFZvsKXicj0
Preprint
Full-text available
Understanding hibernation in brown bears ( Ursus arctos ) can provide insight into many human diseases. During hibernation, brown bears experience states of insulin resistance, physical inactivity, extreme bradycardia, obesity, and the absence of urine production. These states closely mimic human diseases such as type 2 diabetes, muscle atrophy, re...
Article
Full-text available
Numerous environmental and endogenous factors affect the level of genetic diversity in natural populations. Genetic variability is the cornerstone of evolution and adaptation of species. However, currently, more and more plant species and local varieties (landraces) are on the brink of extinction due to anthropopression and climate change. Their pr...
Article
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Ice nucleators are of crucial and important implications in various fields including chemistry, climate, agriculture, and cryobiology. However, the complicated extract and biocompatibility of ice nucleators remain unresolved, and the mechanism of ice nucleation remains largely unknown. Herein, we show that natural nanocrystalline cellulose material...
Article
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The preservative effects of low temperature on biological materials have been long recognised, and cryopreservation is now widely used in biomedicine, including in organ transplantation, regenerative medicine and drug discovery. The lack of organs for transplantation constitutes a major medical challenge, stemming largely from the inability to pres...
Thesis
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Thesis for the degree of Candidate of Medical Sciences in specialty of 14.01.35 - “Cryomedicine”. – Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, 2021. The thesis is devoted to the optimization of wound healing after skin cryoablation by applying a combination of regulatory compoun...
Article
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CryoLetters launched its series of PERSPECTIVES articles in the middle of 2020. They aim to provide an overview of recent developments in cryobiology and cryobiotechnology that will appeal to a wide readership. These mini-reviews are available as Open Access articles, at no cost to the authors. The first five PERSPECTIVES, that are briefly reviewed...
Article
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Experimental reproducibility in aquatic microbial ecology is critical to predict the dynamics of microbial communities. However, controlling the initial composition of naturally occurring microbial communities that will be used as the inoculum in experimental setups is challenging, because a proper method for the preservation of those communities i...
Article
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A biobank may be a form of biorepository that stores biological samples (usually human samples) to be used for diagnostic/ research purpose. Biobank became a key resource, supporting many varieties of up to date research like genomics and personalized medicine since 1990. A Biobank acts as a basement with the elaborated detailed information which a...
Chapter
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From early dry-ice-based freezers and passive coolers, cryopreservation devices have come a long way. With increasing interest in the field of cryobiology from new scientific applications, the importance of reliable, traceable, and reproducible cold chain devices is sure to increase, ensuring more precise cryopreservation and enabling better post-t...
Article
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Aim. To study the effect of different concentrations of ethylene glycol and glycerin in equilibration and vitrifi cation solutions on 1) the viability and further development of frozen-thawed bovine ovarian cumulus-oocyte complexes (COCs), cryopreserved by vitrifi cation method, 2) on the effectiveness of inseminating mature oocytes, obtained from...
Article
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In addition to protecting species in situ, it is crucial to maintain viable populations ex situ (in conservation breeding centers or zoos) for future reintroduction efforts. However, reproduction fitness is impaired in captivity by small space, complexity of husbandry, modified behavior, or infertility. Conservation breeding can therefore be optimi...
Article
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Ice templating – or freeze casting – has flourished in multiple domains as a straightforward process to shape solutions and particle suspensions into macroporous materials. Longtime used as a process to shape colloidal suspensions into lightweight ceramics, the use of ice templating has evolved to fabricate materials that mimic the architecture of...
Article
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Recently the International Society for Biological and Environmental Repositories (ISBER) and the Society for Cryobiology issued the Addendum 1: Liquid Nitrogen-Based Cryogenic Storage of Specimens to the ISBER Best Practices (BP): Recommendations for Repositories, 4th Edition. For this purpose the ISBER Self-Assessment Tool (SAT) has been updated i...
Article
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La crioconservación es una herramienta biotecnológica que en peces está orientada principalmente a la conservación criogénica de semen como estrategia de preservación del recurso genético y a su uso para la producción de alevinos con fines diferentes. Actualmente, los protocolos de crioconservación seminal en peces de agua dulce establecen una ampl...
Article
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Probl Cryobiol Cryomed 2020; 30(3):286 https://doi.org/10.15407/cryo30.03a.286 Морфологічна характеристика ендотелію аорти щурів із хронічною нирковою недостатністю після введення кріоекстракту плаценти щура, застосування медикаментозної блокади РААС та їх поєднаної дії Ю.О. Чиж, М.В. Рєпін, Л.М. Марченко, В.І. Строна, Т.П. Говоруха Інститут пробле...
Article
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Background: Cryopreservation is the best way for long-term in vitro preservation of plant germplasm resources. The preliminary studies found that reactive oxygen species (ROS) induced oxidative stress and ice-induced membrane damage are the fundamental causes of cell death in cryopreserved samples. How to improve plant cryopreservation survival ra...
Article
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Seminal plasma (SP) is the natural environment for spermatozoa and contains a number of components, especially proteins important for successful sperm maturation and fertilization. Nevertheless, in standard frozen stallion insemination doses production, SP is completely removed and is replaced by a semen extender. In the present study, we analyzed...
Article
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Aim. To evaluate the activity of cryopreserved boar ejaculated spermatozoa under different thawing protocols to optimize biotechnological approaches in pig reproduction based on the genetic material of the Institute of Animal Breeding and Genetics nd. a. M.V. Zubets of National Academy of Agrarian Science of Ukraine. Methods. Cryopreserved ejaculat...
Chapter
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It is now approaching 50 years since the first reports were made on cryopreservation of mammalian embryos with successful recoveries and subsequent live births. These successes were the result of growing shared knowledge between the fields of reproductive biology and cryobiology. Once manipulation of human embryos became possible in infertility tre...
Article
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The review presents data on immune pathogenesis of COVID-19 and approaches to its prevention and treatment. The low effectiveness of antiviral drugs is caused by the ability of SARS-CoV-2 virus to change its own structure and functions. Existing treatment strategies of COVID-19 are focused on applying the direct antiviral therapies, modulation of i...
Article
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Glaciers support diverse ecosystems that are largely comprised of microbial life. However, at larger, macroscopic scales, glacier moss balls (sometimes called “glacier mice”) can develop from impurities on ice surfaces and represent a relatively rare biological phenomenon. These ovoid-shaped conglomerations of dirt and moss are only found on some g...
Article
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English version below: Vor dem Hintergrund einer ökonomisch ausgerichteten, beschleunigten, multioptionalen und reflexiven Moderne steht die gelingende Existenzbastelei (HITZLER & HONER 2012 [1994]) des Menschen der Gegenwart in einem Verhältnis zu einem effizienten Umgang mit der eigenen Lebenszeit. Die gesellschaftliche Relevanz eines planbaren...
Preprint
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Background: Cryopreservation is the best way for long-term preservation of plant germplasm resources. The preliminary studies found that ROS-induced oxidative stress and ice-induced membrane damage are the fundamental causes of cell death in cryopreserved samples. How to improve plant cryopreservation survival rate is an important scientific issue...
Preprint
Full-text available
Background: Cryopreservation is the best way for long-term in vitro preservation of plant germplasm resources. The preliminary studies found that reactive oxygen species (ROS) induced oxidative stress and ice-induced membrane damage are the fundamental causes of cell death in cryopreserved samples. How to improve plant cryopreservation survival rat...
Preprint
Full-text available
Background: Cryopreservation is the best way for long-term in vitro preservation of plant germplasm resources. The preliminary studies found that reactive oxygen species (ROS) induced oxidative stress and ice-induced membrane damage are the fundamental causes of cell death in cryopreserved samples. How to improve plant cryopreservation survival rat...
Article
Full-text available
Dimethyl sulfoxide (DMSO) is the cryoprotectant of choice for most animal cell systems since the early history of cryopreservation. It has been used for decades in many thousands of cell transplants. These treatments would not have taken place without suitable sources of DMSO that enabled stable and safe storage of bone marrow and blood cells until...
Article
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This review presents the current progress in and approaches to in vitroconservation of reproductive cells of animals, including birds, such as cryopreservation and freeze-drying, as well as epigenetic conditions for re storing viable spermatozoa and female gametes after conservation. Cryopreservation is an effective way to preserve reproductive cel...
Article
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Sperm cryopreservation represents a powerful tool for livestock breeding. Several efforts have been made to improve the efficiency of sperm cryopreservation in different ruminant species. However, a significant amount of sperm still suffers considerable cryodamage, which may affect sperm quality and fertility. Recently, the use of different “omics”...
Article
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Auto-transplantation of cryopreserved ovarian tissue for cancer survivors comes with the primary concern of the possible existence of cancer cells in the transplanting tissue. Lineal cancer cells are presented by industry in form of separated cells (suspensions). Data about experimental models for evaluation of a cryopreservation effect on viabilit...
Article
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More than 80% of Earth’s surface is exposed periodically or continuously to temperatures below 5 °C. Organisms that can live in these areas are called psychrophilic or psychrotolerant. They have evolved many adaptations that allow them to survive low temperatures. One of the most interesting modifications is production of specific substances that p...
Article
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The larvae of Drosophila melanogaster is a model organism widely used to study the muscular and nervous systems. Drosophila larvae are surrounded by a waxy cuticle that prevents permeation by most substances. Here we develop a method to remove this layer, rendering the larvae permeable to small molecules without causing death, allowing the larvae t...
Article
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Artificial insemination (AI) is widely used in livestock industries to breed for desirable characteristics and increase yields. The standard practice of storing and transporting bovine semen uses liquid nitrogen (LN), a scarce commodity in many regions of the world. This study explored the feasibility of using dry ice, a more readily available alte...