Questions related to Controlled Drug Delivery
I am looking for a plain/empty gel system, which we can directly buy and load the drug for the purpose of transdermal delivery. Your kind help will be appreciated.
Hello, can anyone please named few potential drug delivery technologies that you think are novel and unique. Our comapny is looking to buy few drug delivery technologies that can serve us to compete for now and few next years. Suggestions are highly appreciated. Thanks in advance.
Hi to all!
I am currently working on sequential glucose and oxygen delivery in tissue engineering field and I need to choose specific tissue and cells to work on. I wonder that which tissue need the highest amount of glucose during the first days of implantation. is there any valid reference reporting concentration of normal glucose consumption rate in different tissues?
I have collected 6h to 96h drug release data for unilamellar liposomal vesicle by using spectrophotometer. According to data, we have encapsulated chemotherapeutic agent in 80% efficiency. But when we look at the release profile, liposomes do release the drug at minimum level even at the 96h. Is it possible ? What are the reasons behind that ? Is minimum releasing okay ? What are the consequences of this thightly encapsulation ?
The main (and generalistic) advantages and disadvantages of each type of nanosystem have been widely pointed out in literature. However, the advantages and disadvantages stated are too broad and sometimes overlap. Of course, it can depend on several factors: the targeted tissues; the loaded compounds; the type of administration; the final formulation; industrial scalability; among others. This way, this question aims at opening a debate to untangle and explore further the potentialities of each nanosystem and, more specifically, the (1) differential and (2) more specific applicabilities of each one towards the right final choice for the preconized biomedical application. Personal experiences and opinions are deeply welcomed!
These days the hottest news of the world is about the rate of the victims of Coronavirus disease (COVID-19). It is very interesting if you share your valuable experiences here. Let's help each other.
There are several phytochemicals and natural products reported for having good anticancer or antiviral activity.. but problems like low solubility and degradation on first pass metabolism make them unsuitable for oral administration..
Please share your ideas about compounds belonging to this category
I try to encapsulate a drug (Diclofenac pot.) into a micelle and study its release.
Firstly I use thin film hydration method is this method suitable? Since both drug and polymer are soluble in water.
Secondly; calibration curve of the drug done in solvent or in a buffer (7.4 for example). Because I read many papers and confused which is correct?
Third; how to calculate amount of drug encapsulated and then % of drug release ?
Electrospun fiber mats are normally highly porous. During electrospinning process one can control the pores on individual nanofibers which depends on operating parameters such as relative humidity. How can you benefit from these pores while the average pore size and pore size distribution of the fiber mat is considerably high compared to the individual fiber strands?
I am preparing temperature responsive in situ forming hydrogel loaded with Dox-Liposomes. i need to measure the particle size and zeta potential of hydrogel loaded Dox-Liposomes. I also need to determine EE% of drug from Hydrogel loaded Dox-Liposomes. During particle size measuring, size of hydrogel particle was very higher as compare to Dox-Liposomes. i performed it with dilution of hydrgel -liposomes suspension. Is dilution require for particle size measuring? If require, then at which ratio?
I am searching what is the maximum molecular radius for protein based drug and/ antibody related drug.
If any one know please let me inform.
Thanks in advance.
For injectable application, what is the maximum viscosity of the formulation either microemulsion, or in-situ gel allowed to use? Is there any reference for this viscosity range of injectables?
Do nanocomposite ethosome degrade in the blood? Do ethosomal nano carreier release the drug in the blood? Do these carriers bring the drug into the cells? What factors affect the degradation of ethosomal in the blood?
hello i have question regarding intranasal drug delivery as drug delivered to the brain through nasal route will either bypass BBB (olfactory region) and/or remaining drug will either cleared by mucocilliary clearance or will go into the systemic circulation so i have que that during in-vitro permeability study (franz diffusion cell) which media should i use in acceptar campartment in order to mimic its permeation in brain & blood as well.......
I would like to know about the issues related with the application of metallic nanoparticles in ocular drug delivery. Specially for the drug delivery to the the posterior segment of the eye. Which path they usually follow to cleared out from the posterior segment. What about the toxicity issue due to their possible deposition in the posterior segment if they are not able to move out from there.
I am doing an experiment where I am applying a drug 40 microMolar to the apical dissolved in HBSS (500mcl total), and no drug just 1.5 ml of HBSS to the basolateral and then I measure the concentrations in the basolateral taking 100mcl and replace it with 100mcl HBSS to maintain sink conditions at different time points. and finally get the concentration using HPLC.
now how to calculate the cumulative concentration in the basolateral?!
I've been synthesizing PLGA particles for the controlled release of different drugs. I'm wondering what is the impact of using PVA of different Mw. I've read about the impact of PVA concentration, but is there a optimal Mw?
thank you in advance!
I am looking for the answer how metabolic nanoparticle bind to targeted agent in drug delivery system,& what is the relation with the surface area?
Hi every body, I am going to work in Computational nanomedicine fields, but i do not have any information about it, where should i start? thank you
The project is very innovative and promising for the current and future generations. I appreciate your initiative and novel ideas. I am interested to work on such project as collaboration.
I recently read several articles on single walled carbon nanotube based drug delivery system. However, in this field, multi walled carbon nanotube is less frequently used.
Does multi walled carbon nanotube has any advantages over single walled carbon nanotube in constructing drug delivery?
OR is there any difference about using single walled carbon nanotube or using multi walled carbon nanotube as the carrier for drug delivery.
I am looking for 3D software to generate the 3D bio-images. Except bio-chemdraw software, does anybody know what software is appropriate? I would be so thankful if you provide me with the download link/ purchase link.
I am preparing niosomes from mix of Span 40,60,80 and Cholestrol and I want to use them for drug delivery. It has been long I am struggling with the concentration of ingredients. As I use 100mg for 10 ml of hydration medium which is equal to 2 e-4 mol/L, but the amount is very small to manage ( when weighting) .
My method is thin film hydration method and for now the molarity of Span which I prepare at first ( 50 cc solution of Chloroform/methanol) is 2 e-4 . I just wonder how I can determine the range of concentration which I will have the niosomes.
I want to check the dissolution behavior of a liphophilic drug particles and want to know:
1- The exact dissolution medium
2- Weather or not to use membrane filters (If yes, type and size)
3- Optimal conditions (If changed from normal)
If I prepare Eudragit nanoparticles and later convert it to suspension and use it as nasal spray, would it be ok? Remember drug is neither for respiratory tract, nor it has to target brain. I have not seen any article so far in which eudragit alone has been used in the nasal route.
I'm doing drug release using Franz diffusion cell. The medium is PBS. I'm taking 900uL samples at predetermined times and replace the amount taken with fresh PBS to maintain the sink condition. I'm not sure how to do a cumulative release plot (amount permeated vs time) because the absorbance data that I've got is going up and down( is that normal?).
Spesifically, for encapsulation protocol of hydrophobic drugs into PAMAM dendrimers, I need to optimize the required/adequate time to remove the non-encapsulated drug by dialysis against DI water. I'm trying to understand how to set the time before the system starts to release the encapsulated drug.
I'd be very happy if you could help.
The evaporation is only 100uL, but buffer solution is only 1mL and membrane is dried after 24 hours.
Attached photo and link
We need to inject drugs into rat brain regularly, but that would do too much damage given to the needed frequency. We tried nano particle strategy and those commercial SR pumps, but they didn't work well in our experiments. So I wonder if there's any other validated ways to inject the drug once and let it perform sustained release itself. Many thanks.
I see some papers have 10% w/v tween 80 or even more, but if we increase the concentration to 10% doesn't the dissolved drug or the particle get adhered to the tube? and also that might have some effects on the dialysis membrane?
Please correct me if I am wrong.
Hi, I am working upon nano medicines. Drugs are made targeted to the site of action. So, I am wondering for how a drug can be made a targeted one. How does it reach to specific area without prior loss or being harmed?
I'm curious about different delivery methods for chronic THC exposure in adolescent rats (3 weeks). Typically, intraperitoneal injections of THC are used, but I'm wondering if there is an easier/less stressful method.
Some ideas I'm considering include:
1. Osmotic minipump implants. This might be the simplest method.
2. Food delivery. Mixing THC with a palatable food like strawberry/chocolate milkshake, or jello, with appropriate pre-exposure of drug and vehicle to avoid conditioned taste aversion. I know jello has been used with alcohol studies.
3. Catheter implantation. Daily intravenous delivery of THC would allow for very precise control of drug delivery according to daily body weight.
I'd be very curious to hear of any ideas others have tried.
Dear Folks, struggling for a good intra-nasal delivery of vaccine although my vaccine is working perfectly by SC and IM routes. Tried with same IN route but is not working. Could you please suggest about the possible excipients or compounding materials to formulate, so it will be staying into the nasal cavity or thereby lungs for a while. It's need to be stayed as well as uptaken by antigen presenting cells, macrophages or other immune cells for better IgA or IgG response. Do I need to think about something to be included in the vaccine that can help or enhance the penetration of intranasal membrane barrier or mucous around the nostril's or nasal cavity? Your advice or suggestions will be highly appreciated. Thanks.
Attached Figure is showing the thermal release of entrapped drug. Free drug, Free nano-particle and entrapped drug (Np+drug) is determined at different temperature (25-300 C). Why the drug lose its UV-Vis absorbance when it was entrapped in nano-particle?!!?
The purpose of the mini-pump is to deliver in a sustained manner the compound known as 7-OHDPAT over a period of 2 months...
Thanks in advance for your comments!!
Attempting to find the Encapsulation Efficiency%, by dissolving the drug loaded matrix in a suitable solvent,the color of the drug is visible on the matrix surface even after several washes.
can anyone please mention a very simple method to study the drug release from an electrospun PLGA mesh by taking in conservation that it's hydrophobic
Also tell how much volume of liquid should be used to redisperese them. How much amount of sample should be taken to redispere in liquid. Kindly give a detailed reply
I have been trying to deliver a very small (# 3 kDa) and hydrophobic peptide into cells in order to study on its biological function in neurons and pc12 cells using different methods, but I found that the efficiency was very low <5%. Recently, I am advised to use micelles for that purpose, so I am just wondering if anyone has some experience about micelles and can give me some advice?
i am preparing nanoparticles, drug is hydrophilic and entrapment efficiency is very low. If i find the drug release by dividing with the total drug taken its giving very low drug release so is it possible to find drug release by dividing with the total drug entrapped? Kindly send if there is any reference for it.
We are working on an intracellular delivery technology and have verified cytosolic delivery of a membrane impermeable dye and dextrans, but are having trouble with GFP plasmids and need to check if we are getting cytosolic delivery. Any tips on how to do this?
The use of Organic Solvents in performing the release studies for Hydrophobic drugs is commonly seen. The main aim of the study is to see the release pattern of the drug from the formulation and to have some correlation with its in-vivo behaviour.
Most of the time we see similar methods used in publications for performing the release studies, irrespective of the type of formulation and route of administration, which is not convincing.
I would also like to know how we can design release studies in such a way that it mimics the in-vivo conditions.
Finally, can we justify the use of organic solvents and surfactants for in-vitro release studies and correlate with in-vivo behaviour?
I am wondering whether there are some experts in osmotic pump field who may kindly help me with the following questions:
What is the most important parameters involved in controlling the internal pressure? What kind of material could be used to make the "outer coat", only allowing for water to traverse?
Am currently working with NIVs and am struggling to understand why, if discovered in the 1970s and first utilised as a drug delivery system (DDS) in 1985 (Chemotheraphy), why has their use as a DDS not been more widely used? Only recently (2012), has their use as a DDS been a primary focus with intention to move into human trials. Has it been a technological barrier? (I.e. manufacturing at a commercial scale) or something else?
Any answers or resource you can direct me to would be amazing.
Thanks in advance.
Greetings to everyone,
I want to deliver siRNA to Cardiomyocytes (Heart) using liposomes or polymeric nanoparticles or any other drug delivery vehicle.
For the targeted delivery to cardiomyocytes, I am looking for some specific protein which are specifically expressed or over expressed on surface of heart muscles. I would be glad to know the appropriate size of drug delivery vehicle to deliver the content in heart muscles.
So please suggest me some appropriate marker to target the cardiomyocytes using drug delivery vehicles.
Thank you in advance :)
Nowadays so many research articles only indicate their compound treatment group only. they do not compare with any standard drug. kindly send the reasons
There are many protocols available, but unfortunately the solvent used give a high background reading and therefore i can not detect my drug.
Which method is more useful when measuring the encapsulation efficiency using UV/VIS at 360nm?
Thanks in advanced!
I am working on Phytosomal drug delivery of herbal extracts. I am finding difficulty in getting phytosome using methanolic extract of plants. Please suggest the reference or your experience in Phytosomal preparion using Lecithin.
I need to buy some transdermal patches for Nicotine, Fentanyl, Nitroglycerin, Scopolamine, Sumatriptan generic one for research purpose. Preferably want to buy from India as I am going to visit there. Also anyone has done any experiment on this type of patches will be appreciated to contact.
I am preparing nanoparticles by w/o/w double emulsion method.Firstly I am sonicating w/o emulsion at 70 % amplitude for 2 minutes by using sonics vibracell ultrasonic processor 500 W, 20 kHz and then again sonicating the w/o/w emulsion for 2 minutes at 70 % amplitude using sonics vibracell ultrasonic processor 500 W, 20 kHz. Is it right?
To study release of a hydrophobic drug, I need a method that may to evaluate how much drug dissolves in PBS in time? I have some difficulties to obtain good results using UV-vis, can someone recommend any articles on this topic?
After collecting supernatant which pore size filter is used to filter the supernatant? After filtration do we take its UV absorbance or to do anything in between? After taking its absorbance what to do next?
Using stealth liposome is one of the best option to target cancer cells but it also destroys host cells because liposomes are not able to differentiate exactly between cancer cells and host cells. Targeting through antibody mediated liposomal drug delivery specific for cancer surface receptors may have some immunological complexions. Is there any other method to avoid immune reaction from host? Or Is there any method to specifically target cancer cells? Any Ideas/Suggestions are welcome...
It has become very common practice with supplements to administer some through the Buccal delivery method (sublingually). In fact, Resveratrol is best absorbed when taken under the tongue and when bypassed the gastrointestinal tract totally.
My question, can you put cycloastragenol powder underneath the tongue for better absorption into the bloodstream and better activation of telomerase ? Is Cycloastragenol one of the supplements that can be absorbed through the lining of the mouth? Thank you, Brooks
Dear all, Are there anyone send me a standard (ASTM) procedure to determine the solubility of a prepared polymer at aqueous buffer solution?
We are trying to prepare an aqueous soluble polyimide for using as an enteric coating for controlled drug delivery.
Generally nanoemulsion is prepared by first calculating solubility of drug in oil phase then further titrating it using water and the system stabilized by a surfactant. I want to know the preparation procedure if i am using a drug that is in oily state (for example clove oil). Will i have to perform any solubility study when my drug itself acts as an oil phase?
Many works have been reported so far on various drugs belonging to many categories like anihypetensives, anti-lipidemia, etc etc. But i want to know if any of the drugs have been marketed?
If I prepare Eudragit nanoparticles and later convert it to suspension and use it as nasal spray, would it be ok? Remember drug is neither for respiratory tract, or it has to target brain. I have not seen any article so far in which eudragit alone has been used in the nasal route.
load drug-silver nano particles composite inside superporous hydrogel structure.