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Comparative Physiology - Science topic
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I use light protected jars and big falcon tubes to grow my plants. Usually, I don't get algal contamination when growing plants for 2-3 weeks. But, sometimes I get algal growth in my samples. It would be great to have your views on following questions.
1. Is it common?
2. How do you deal with it?
3. How much impact it can have on normal growth and development, if I am regularly changing the media every 2 days?
4. Can I use MICROPUR CLASSIC MC 10T tablets?
5. Although, hydroponics is a non sterile system; is it okay to consider this system for comparative physiological & molecular studies if I am getting mild algal growth once in a while?
I am so confused as I found out that some sources mentioned Y. lipolytica can accumulate lipid up to 36% of DCW. But there are also some sources mentioned that the wild-type strain of the yeast could not accumulate lipid more than 20% of DCW.
So, why is Y. lipolytica still considered as an oleaginous microbe?
Following the recent International Symposium on Fish Endocrinology in Buenos Aires, the question was raised within the international committee if a Society for Fish Endocrinology (SFE) should be created. The president of the committee, Olivier Kah, has therefore sent out the attached survey together with a background explanation to the idea. Everybody is encouraged to fill out the survey and send to Olivier Kah by e-mail.
I have 26 different physiological presentations. These groups range from 159 individuals to just a single individual. The test result can either be positive, negative, or variation of unknown significance. I'd like to compare the physiological presentations (groupings) to find for which presentations the test is most likely to yield a positive result.
My first inclination was to do a chi-squared analysis. But there are far too many groups with an expected result less than 5. The Fischer Exact test seems like a poor choice given the sheer number of groups. My next choice was making the population simply the individuals in the top 6 most common physiological presentations. This would give me >80% of groups with a count over five.
Regardless, it seems like the chi-squared test is probably the least powerful test at my disposal. Perhaps a more useful test would be testing whether the groups (ordered by positive frequency) is the true order?
I tried GA treatment and HNO3 (scarification) but was in vain.
Fish larvae (and mostly also juveniles) are too small to draw blood. Can anyone point me to literature how to assess stress levels in fish larvae not involving RNA/DNA ratios?
A large number of murine studies report HOMA-IR alongside IPGTT/OGTT and fasting glucose/insulin levels as a proxy of systemic insulin resistance. However, is this a valid approach? How applicable is the HOMA-IR calculation to murine physiology?
For safety reasons (to kill potentially infective micro-organisms) I would like to irradiate faecal samples (from various mammalian species) before performing enzyme immunoassays for faecal glucocorticoid metabolites. I use freeze-drying, followed by a chemical extraction method (using 80% methanol) to extract the GC hormones from the faeces. I would like to do the irradiation before the freeze-drying step. I am currently using both cortisol and corticosterone EIAs on the extracts. Is the irradiation likely to affect the EIA results?
AFLP, Nearest, DarT, SNP. How can we normalize them into one consensus map?
I've got a series of non-linear functions for which I want to calculate the auc at a series of x-intervals automatically and populate a data frame. I can calculate the auc values as one-off estimates, but I'm having trouble setting a code to calculate them automatically for an atomic vector. I.e, I can't work out the code to calculate auc in reference to a column of x-values that are already in my target data frame. I can't be the first person to need to do this, so any insights will be greatly appreciated. Cheers
In human when we measure ( ECG) there are ( P, Q ,R , S , and T wave but in chicken the Q wave are absent why ?
I am in need of normal reference values (like we have for humans,) for various biochemical parameters in adult male albino wistar rats, like serum glucose,plasma insulin, HbA1C, SGOT, SGPT, ALP,UREA, CREATININE, ALBUMIN, LDL, HDL,TOTAL CHOLESTEROL,TRIGLYCERIDES, VLDL and in vivo liver antioxidant enzymes like SOD,CAT,GPX ,LPO. Please provide me the values with proper references. thanks.
The goal would be to determine (under lab conditions) differences in the reproductive success after 5-6 months of winter hibernation, of female yearlings born either early or late during the previous year.
I encountered m1's of Microtus oeconomus (n=3) and m1's of Microtus gregalis (n=4) in a small faunule, also containing Lemmus sp. and Dicrostonyx sp., in the Netherlands. From 3 upper M2, 1 has a posterior appendage, completely isolated from T3, looking like a M2 of Microtus agrestis.
Gromov and Polyakov (1977) mention on page 397 of the english edition(1992) about Microtus gregalis, the following: "M1-M2 in individuals of some populations tend toward formation of additional lobes at posterior end and on M1 toward isolation of complete triangles from second lobe of paraconid section."
No figure or picture is presented with this phenomenon. So I don't know if this looks like a M. agrestis loop. Does anyone know a picture of a M2 of M. gregalis with the above mentioned features? Then I can compare and perhaps see if the M2 is of M.agrestis or M. gregalis.
It would be interesting to know if other species are prone to Diabetes Typ 1 for example.
Humans aren’t the only ones who lose their hearing as they grow older. Scientists report that wild Indo-Pacific humpback dolphins (Sousa chinensis), which can live 40-plus years, also have trouble picking up sounds as they age.
I would be interested in providing tissues to a lab that has experience imaging or otherwise quantifying mitochondrial density in whole muscle, liver, heart, etc.
Anyone know of differences in isosbestic points for fully oxygenated vs. fully deoxygenated isolated hemoglobin in marine mammal species?
